Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,169,642	G→A	A12V (GCC→GTC)	ygiW ←	BOF family protein YgiW

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,169,642	0	G	A	100.0%	268.9 / NA	76	A12V (GCC→GTC)	ygiW	BOF family protein YgiW
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (29/47);  total (29/47)

GTCACGCAGGGATTTTGCGCTTTCTACAGTCGTTACGCTGCCGTTCGGCCCCTGGAATCCTCCGGCCTGACTTTGCGTTGCCGATGGGCCAGAAAAACCGCCCTGCTCTGCTGCCATCACCGGTGCGCTGCACAGGGCCATTACTGCGATTACTGCTGCGAATTTTTTCATGTTTATTACTCCCTTTAATGTCTGTTTCCGAGCATTTAACAAGATAGTCCTTAACAACTTCTTAAGGGAAAAAAATAAAATTTAGTGCTGTACAGAGCG  >  NC_000913/3169506‑3169775                                                                                                                                         |                                                                                                                                      gtcACGCAGGGATTTTGCGCTTTCTACAGTCGTTACGCTGCCGTTCGGCCCCTGGAATCCTCGGGCCTGACTTTGCGTTGCCGATGGGCCAGAAAAACCGCCCTGCTCTGCTGCCATCACCGGTGCGCTGCACAGGACCa                                                                                                                                    >  2:1258985/1‑140 (MQ=255) gtcACGCAGGGATTTTGCGCTTTCTACAGTCGTTACGCTGCCGTTCGGCCCCTGGAATCCTCCGGCCTGACTTTGCGTTGCCGATGGGCCAGAAAAACCGCCCTGCTCTGCTGCCATCACCGGTGCGCTGCACAGGACCa                                                                                                                                    >  2:1071039/1‑140 (MQ=255) gtcACGCAGGGATTTTGCGCTTTCTACAGTCGTTACGCTGCCGTTCGGCCCCTGGAATCCTCCGGCCTGACTTTGCGTTGCCGATGGGCCAGAAAAACCGCCCTGCTCTGCTGCCATCACCGGTGCGCTGCACAGGACCa                                                                                                                                    >  2:889867/1‑140 (MQ=255) gtcACGCAGGGATTTTGCGCTTTCTACAGTCGTTACGCTGCCGTTCGGCCCCTGGAATCCTCCGGCCTGACTTTGCGTTGCCGATGGGCCAGAAAAACCGCCCTGCTCTGCTGCCATCACCGGTGCGCTGCACAGGACCa                                                                                                                                    >  2:308775/1‑140 (MQ=255)          ggATTTTGTGCTTTCTACAGTCGTTACGCTGCCGTTCGGCCCCTGGAATCCTCCGGCCTGACTTTGCGTTGCCGATGGGCCAGAAAAACCGCCCTGCTCTGCTGCCATCACCGGTGCGCTGCACAGGACCATTACTGCGa                                                                                                                           >  2:1342835/1‑140 (MQ=255)                      ttCTACAGTCGTTACGCTGCCGTTCGGCCCCTGGAATCCTCCGGCCTGACTTTGCGTTGCCGATGGGCCAGAAAAACCGCCCTGCTCTGCTGCCATCACCGGTGCGCTGCACAGGACCATTACTGCGATTACTGCTGCGa                                                                                                               >  2:319133/1‑140 (MQ=255)                            aGTCGTTACGCTGCCGTTCGGCCCCTGGAATCCTCCGGCCTGACTTTGCGTTGCCGATGGGCCAGAAAAACCGCCCTGCTCTGCTGCCATCACCGGTGCGCTGCACAGGACCATTACTGCGATTACTGCTGCGAAttttt                                                                                                         <  1:1342835/140‑1 (MQ=255)                              tCGTTACGCTGCCGTTCGGCCCCTGGAATCCTCCGGCCTGACTTTGCGTTGCCGATGGGCCAGAAAAACCGCCCTGCTCTGCTGCCATCACCGGTGCGCTGCACAGGACCATTACTGCGATTACTGCTGCGAATTTTTTc                                                                                                       <  2:1164143/140‑1 (MQ=255)                              tCGTTACGCTGCCGTTCGGCCCCTGGAATCCTCCGGCCTGACTTTGCGTTGCCGATGGGCCAGAAAAACCGCCCTGCTCTGCTGCCATCACCGGTGCGCTGCACAGGACCATTACTGCGATTACTGCTGCGAATTTTTTc                                                                                                       <  1:749641/140‑1 (MQ=255)                                gTTACGCTGCCGTTCGGCCCCTGGAATCCTCCGGCCTGACTTTGCGTTGCCGATGGGCCAGAAAAACCGCCCTGCTCTGCTGCCATCACCGGTGCGCTGCACAGGACCATTACTGCGATTACTGCTGCGAATTTTTTCAt                                                                                                     <  1:790720/140‑1 (MQ=255)                                 ttACGCTGCCGTTCGGCCCCTGGAATCCTCCGGCCTGACTTTGCGTTGCCGATGGGCCAGAAAAACCGCCCTGCTCTGCTGCCATCACCGGTGCGCTGCACAGGACCATTACTGCGATTACTGCTGCGAATTTTTTCATg                                                                                                    <  1:1064652/140‑1 (MQ=255)                                 ttACGCTGCCGTTCGGCCCCTGGAATCCTCCGGCCTGACTTTGCGTTGCCGATGGGCCAGAAAAACCGCCCTGCTCTGCTGCCATCACCGGTGCGCTGCACAGGACCATTACTGCGATTACTGCTGCGAATTTTTTCATg                                                                                                    >  2:348338/1‑140 (MQ=255)                                 ttACGCTGCCGTTCGGCCCCTGGAATCCTCCGGCCTGACTTTGCGTTGCCGATGGGCCAGAAAAACCGCCCTGCTCTGCTGCCATCACCGGTGCGCTGCACAGGACCATTACTGCGATTACTGCTGCGAATTTTTTCATg                                                                                                    >  2:806179/1‑140 (MQ=255)                                 ttACGCTGCCGTTCGGCCCCTGGAATCCTCCGGCCTGACTTTGCGTTGCCGATGGGCCAGAAAAACCGCCCTGCTCTGCTGCCATCACCGGTGCGCTGCACAGGACCATTACTGCGATTACTGCTGCGAATTTTTTCATg                                                                                                    >  2:920701/1‑140 (MQ=255)                                   aCGCTGCCGTTCGGCCCCTGGAATCCTCCGGCCTGACTTTGCGTTGACGATGGGCCAGAAAAACCGCCCTGCTCTGCTGCCATCACCGGTGCGCTGCACAGGACCATTACTGCGATTACTGCTGCGAATTTTTTCATGtt                                                                                                  >  2:1147788/1‑140 (MQ=255)                                        gCCGTTCGGCCCCTGGAATCCTCCGGCCTGACTTTGCGTTGCCGATGGGCCAGAAAAACCGCCCTGCTCTGCTGCCATCACCGGTGCGCTGCACAGGACCATTACTGCGATTACTGCTGCGAATTTTTTCAt                                                                                                     <  1:541778/132‑1 (MQ=255)                                        gCCGTTCGGCCCCTGGAATCCTCCGGCCTGACTTTGCGTTGCCGATGGGCCAGAAAAACCGCCCTGCTCTGCTGCCATCACCGGTGCGCTGCACAGGACCATTACTGCGATTACTGCTGCGAATTTTTTCAt                                                                                                     >  2:541778/1‑132 (MQ=255)                                        gCCGTTCGGCCCCTGGAATCCTCCGGCCTGACTTTGCGTTGCCGATGGGCCAGAAAAACCGCCCTGCTCTGCTGCCATCACCGGTGCGCTGCACAGGACCATTACTGCGATTACTGCTGCGAATTTTTTCATGTttatta                                                                                             <  2:236577/140‑1 (MQ=255)                                         ccGTTCGGCCCCTGGAATTCTCCGGCCTGACTTTGCGTTGCCGATGGGCCAGAAAAACCGCCCTGCTCTGCTGCCATCACCGGTGCGCTGCACAGGACCATTACTGCGATTACTGCTGCGAATTTTTTCATGTTTATTAc                                                                                            >  2:233127/1‑140 (MQ=255)                                         ccGTTCGGCCCCTGGAATCCTCCGGCCTGACTTTGCGTTGCCGATGGGCCAGAAAAACCGCCCTGCTCTGCTGCCATCACCGGTGCGCTGCACAGGACCATTACTGCGATTACTGCTGCGAATTTTTTCATGTTTATTAc                                                                                            <  1:525843/140‑1 (MQ=255)                                         ccGTTCGGCCCCTGGAATCCTCCGGCCTGACTTTGCGTTGCCGATGGGCCAGAAAAACCGCCCTGCTCTGCTGCCATCACCGGTGCGCTGCACAGGACCATTACTGCGATTACTGCTGCGAATTTTTTCATGTTTATTAc                                                                                            <  1:348338/140‑1 (MQ=255)                                         ccGTTCGGCCCCTGGAATCCTCCGGCCTGACTTTGCGTTGCCGATGGGCCAGAAAAACCGCCCTGCTCTGCTGCCATCACCGGTGCGCTGCACAGGACCATTACTGCGATTACTGCTGCGAATTTTTTCATGTTTATTAc                                                                                            <  1:530516/140‑1 (MQ=255)                                          cGTTCGGCCCCTGGAATCCTCCGGCCTGACTTTGCGTTGCCGATGGGCCAGAAAAACCGCCCTGCTCTGCTGCCATCACCGGTGCGCTGCACAGGACCATTACTGCGATTACTGCTGCGAATTTTTTCATGTTTATTACt                                                                                           <  2:531993/140‑1 (MQ=255)                                           gTTCGGCCCCTGGAATCCTCCGGCCTGACTTTGCGTTGCCGATGGGCCAGAAAAACCGCCCTGCTCTGCTGCCATCACCGGTGCGCTGCACAGGACCATTACTGCGATTACTGCTGCGAATTTTTTCATGTTTATTACTc                                                                                          <  1:202944/140‑1 (MQ=255)                                                 ccccTGGAATCCTCCGGCCTGACTTTGCGTTGCCGATGGGCCAGAAAAACCGCCCTGCTCTGCTGCCATCACCGGTGCGCTGCACAGGACCATTACTGCGATTACTGCTGCGAATTTTTTCATGTTTATTACTCCCTTTa                                                                                    <  2:206128/140‑1 (MQ=255)                                                      ggAATCCTCCGGCCTGACTTTGCGTTGCCGATGGGCCAGAAAAACCGCCCTGCTCTGCTGCCATCACCGGTGCGCTGCACAGGACCATTACTGCGATTACTGCTGCGAATTTTTTCATGTTTATTACTc                                                                                          >  1:937002/1‑129 (MQ=255)                                                      ggAATCCTCCGGCCTGACTTTGCGTTGCCGATGGGCCAGAAAAACCGCCCTGCTCTGCTGCCATCACCGGTGCGCTGCACAGGACCATTACTGCGATTACTGCTGCGAATTTTTTCATGTTTATTACTc                                                                                          <  2:937002/129‑1 (MQ=255)                                                              ccGGCCTGACTTTGCGTTGCCGATGGGCCAGAAAAACCGCCCTGCTCTGCTGCCATCACCGGTGCGCTGCACAGGACCATTACTGCGATTACTGCTGCGAATTTTTTCATGTTTATTACTCCCTTTAATGTCTGTTTCCg                                                                       <  2:1311317/140‑1 (MQ=255)                                                               cGGCCTGACTTTGCGTTGCCGATGGGCCAGAAAAACCGCCCTGCTCTGCTGCCATCACCGGTGCGCTGCACAGGACCATTACTGCGATTACTGCTGCGAATTTTTTCATGTTTATTACTCCCTTTAATGTc                                                                               <  1:124245/131‑1 (MQ=255)                                                               cGGCCTGACTTTGCGTTGCCGATGGGCCAGAAAAACCGCCCTGCTCTGCTGCCATCACCGGTGCGCTGCACAGGACCATTACTGCGATTACTGCTGCGAATTTTTTCATGTTTATTACTCCCTTTAATGTc                                                                               >  2:124245/1‑131 (MQ=255)                                                               cGGCCTGACTTTGCGTTGCCGATGGGCCAGAAAAACCGCCCTGCTCTGCTGCCATCACCGGTGCGCTGCACAGGACCATTACTGCGATTACTGCTGCGAATTTTTTCATGTTTATTACTCCCTTTAATGTCTGTTTCCGa                                                                      >  1:672009/1‑140 (MQ=255)                                                               cGGCCTGACTTTGCGTTGCCGATGGGCCAGAAAAACCGCCCTGCTCTGCTGCCATCACCGGTGCGCTGCACAGGACCATTACTGCGATTACTGCTGCGAATTTTTTCATGTTTATTACTCCCTTTAATGTCTGTTTCCGa                                                                      >  1:1293566/1‑140 (MQ=255)                                                                ggCCTGACTTTGCGTTGCCGATGGGCCAGAAAAACCGCCCTGCTCTGCTGCCATCACCGGTGCGCTGCACAGGACCATTACTGCGATTACTGCTGCGAATTTTTTCATGTTTATTACTCCCTTTAATGTCTGTTTCCGAg                                                                     <  1:308775/140‑1 (MQ=255)                                                                ggCCTGACTTTGCGTTGCCGATGGGCCAGAAAAACCGCCCTGCTCTGCTGCCATCACCGGTGCGCTGCACAGGACCATTACTGCGATTACTGCTGCGAATTTTTTCATGTTTATTACTCCCTTTAATGTCTGTTTCCGAg                                                                     <  1:806179/140‑1 (MQ=255)                                                                ggCCTGACTTTGCGTTGCCGATGGGCCAGAAAAACCGCCCTGCTCTGCTGCCATCACCGGTGCGCTGCACAGGACCATTACTGCGATTACTGCTGCGAATTTTTTCATGTTTATTACTCCCTTTAATGTCTGTTTCCGAg                                                                     <  1:920701/140‑1 (MQ=255)                                                                 gCCTGACTTTGCGTTGCCGATGGGCCAGAAAAACCGCCCTGCTCTGCTGCCATCACCGGTGCGCTGCACAGGACCATTACTGCGATTACTGCTGCGAATTTTTTCATGTTTATTACTCCCTTTAATGTCTGTTTCCGAGc                                                                    >  2:330969/1‑140 (MQ=255)                                                                  ccTGACTTTGCGTTGCCGATGGGCCAGAAAAACCGCCCTGCTCTGCTGCCATCACCGGTGCGCTGCACAGGACCATTACTGCGATTACTGCTGCGAATTTTTTCATGTTTATTACTCCCTTTAATGTCTGTTTCCGAGCa                                                                   <  1:889867/140‑1 (MQ=255)                                                                  ccTGACTTTGCGTTGCCGATGGGCCAGAAAAACCGCCCTGCTCTGCTGCCATCACCGGTGCGCTGCACAGGACCATTACTGCGATTACTGCTGCGAATTTTTTCATGTTTATTACTCCCTTTAATGTCTGTTTCCGAGCa                                                                   <  1:330969/140‑1 (MQ=255)                                                                  ccTGACTTTGCGTTGCCGATGGGCCAGAAAAACCGCCCTGCTCTGCTGCCATCACCGGTGCGCTGCACAGGACCATTACTGCGATTACTGCTGCGAATTTTTTCATGTTTATTACTCCCTTTAATGTCTGTTTCCGAGCa                                                                   >  2:320940/1‑140 (MQ=255)                                                                  ccTGACTTTGCGTTGACGATGGGCCAGAAAAACCGCCCTGCTCTGCTGCCATCACCGGTGCGCTGCACAGGACCATTACTGCGATTACTGCTGCGAATTTTTTCATGTTTATTACTCCCTTTAATGTCTGTTTCCGAGCa                                                                   <  1:1147788/140‑1 (MQ=255)                                                                     gACTTTGCGTTGCCGATGGGCCAGAAAAACCGCCCTGCTCTGCTGCCATCACCGGTGCGCTGCACAGGACCATTACTGCGATTACTGCTGCGAATTTTTTCATGTTTATTACTCCCTTTAATGTCTGTTTCCGAGCAttt                                                                <  1:705492/140‑1 (MQ=255)                                                                      aCTTTGCGTTGCCGATTGGCCAGAAAAACCGCCCTGCTCTGCTGCCATCACCGGTGCGCTGCACAGGACCATTACTGCGATTACTGCTGCGAATTTTTTCATGTTTATTACTCCCTTTAATGTCTGTTTCCGAGCATTTa                                                               >  2:75426/1‑140 (MQ=255)                                                                       cTTTGCGTTGCCGATGGGCCAGAAAAACCGCCCTGCTCTGCTGCCATCACCGGTGCGCTGCACAGGACCATTACTGCGATTACTGCTGCGAATTTTTTCATGTTTATTACTCCCTTTAATGTCTGTTTCCGAGCATTTaa                                                              <  2:409640/140‑1 (MQ=255)                                                                       cTTTGCGTTGCCGATGGGCCAGAAAAACCGCCCTGCTCTGCTGCCATCACCGGTGCGCTGCACAGGACCATTACTGCGATTACTGCTGCGAATTTTTTCATGTTTATTACTCCCTTTAATGTCTGTTTCCGAGCATTTaa                                                              <  1:1309018/140‑1 (MQ=255)                                                                            cGTTGCCGATGGGCCAGAAAAACCGCCCTGCTCTGCTGCCATCACCGGTGCGCTGCACAGGACCATTACTGCGATTACTGCTGCGAATTTTTTCATGTTTATTACTCCCTTTAATGTCTGTTTCCGAGCATTTAACAAGa                                                         <  1:589068/140‑1 (MQ=255)                                                                              ttGCCGATGGGCCAGAAAAACCGCCCTGCTCTGCTGCCATCACCGGTGCGCTGCACAGGACCATTACTGCGATTACTGCTGCGAATTTTTTCATGTTTATTACTCCCTTTAATGTCTGTTTCCGAGCATTTAACAAGATa                                                       <  1:469593/140‑1 (MQ=255)                                                                                gcctaTGGGCCAGAAAAACCGCCCTGCTCTGCTGCCATCACCGGTGCGCTGCACAGGACCATTACTGCGATTACTGCTGCGAATTTTTTCATGTTTATTACTCCCTTTAATGTCTGTTTCCGAGCATTTAACAAGATAGt                                                     >  1:987802/5‑140 (MQ=255)                                                                                gCCGATTGGCCAGAAAAACCGCCCTGCTCTGCTGCCATCACCGGTGCGCTGCACAGGACCATTACTGCGATTACTGCTGCGAATTTTTTCATGTTTATTACTCCCTTTAATGTCTGTTTCCGAGCATTTAACAAGATAGt                                                     >  1:209520/1‑140 (MQ=255)                                                                                 ccGATGGGCCAGAAAAACCGCCCTGCTCTGCTGCCATCACCGGTGCGCTGCACAGGACCATTACTGCGATTACTGCTGCGAATTTTTTCATGTTTATTACTCCCTTTAATGTCTGTTTCCGAGCATTTAACAAGATAGTc                                                    >  2:1040014/1‑140 (MQ=255)                                                                                 ccGATGGGCCAGAAAAACCGCCCTGCTCTGCTGCCATCACCGGTGCGCTGCACAGGACCATTACTGCGATTACTGCTGCGAATTTTTTCATGTTTATTACTCCCTTTAATGTCTGTTTCCGAGCATTTAACAAGATAGTc                                                    >  1:345282/1‑140 (MQ=255)                                                                                  cGATGGGCCAGAAAAACCGCCCTGCTCTGCTGCCATCACCGGTGCGCTGCACAGGACCATTACTGCGATTACTGCTGCGAATTTTTTCATGTTTATTACTCCCTTTAATGTCTGTTTCCGAGCATTTAACAAGATAGTcc                                                   >  2:1271370/1‑140 (MQ=255)                                                                                       ggCCAGAAAAACCGCCCTGCTCTGCTGCCATCACCGGTGCGCTGCACAGGACCATTACTGCGATTACTGCTGCGAATTTTTTCATGTTTATTACTCCCTTTAATGTCTGTTTCCGAGCATTTAACAAGATAGTCCTTaac                                              <  2:619889/140‑1 (MQ=255)                                                                                                             tgctgcCATCACCGGTGCGCTGCACAGGACCATTACTGCGATTACTGCTGCGAATTTTTTCATGTTTATTACTCCCTTTAATGTCTGTTTCCGAGCATTTAACAAGATAGTCCTTAACAACTTCTTAAGGGAAAaaaata                        <  1:320940/140‑1 (MQ=255)                                                                                                                  ccATCACCGGTGCGCTGCACAGGACCATTACTGCGATTACTGCTGCGAATTTTTTCATGTTTATTACTCCCTTTAATGTCTGTTTCCGAGCATTTAACAAGATAGTCCTTAACAACTTCTTAAGGGAAAAAAATAAAAtt                   <  2:443719/140‑1 (MQ=255)                                                                                                                  ccATCACCGGTGCGCTGCACAGGACCATTACTGCGATTACTGCTGCGAATTTTTTCATGTTTATTACTCCCTTTAATGTCTGTTTCCGAGCATTTAACAAGATAGTCCTTAACAACTTCTTAAGGGAAAAAAATAAAAtt                   <  1:1267485/140‑1 (MQ=255)                                                                                                                  ccATCACCGGTGCGCTGCACAGGACCATTACTGCGATTACTGCTGCGAATTTTTTCATGTTTATTACTCCCTTTAATGTCTGTTTCCGAGCATTTAACAAGATAGTCCTTAACAACTTCTTAAGGGAAAAAAATAAAAtt                   <  1:1071039/140‑1 (MQ=255)                                                                                                                  ccATCACCGGTGCGCTGCACAGGACCATTACTGCGATTACTGCTGCGAATTTTTTCATGTTTATTACTCCCTTTAATGTCTGTTTCCGAGCATTTAACAAGATAGTCCTTAACAACTTCTTAAGGGAAAAAAATAAAAtt                   <  1:1040014/140‑1 (MQ=255)                                                                                                                  ccATCACCGGTGCGCTGCACAGGACCATTACTGCGATTACTGCTGCGAATTTTTTCATGTTTATTACTCCCTTTAATGTCTGTTTCCGAGCATTTAACAAGATAGTCCTTAACAACTTCTTAAGGGAAAAAAATAAAAtt                   <  1:398515/140‑1 (MQ=255)                                                                                                                  ccATCACCGGTGCGCTGCACAGGACCATTACTGCGATTACTGCTGCGAATTTTTTCATGTTTATTACTCCCTTTAATGTCTGTTTCCGAGCATTTAACAAGATAGTCCTTAACAACTTCTTAAGGGAAAAAAATAAAAtt                   <  1:923708/140‑1 (MQ=255)                                                                                                                  ccATCACCGGTGCGCTGCACAGGACCATTACTGCGACTACTGCTGCGAATTTTTTCATGTTTATTACTCCCTTTAATGTCTGTTTCCGAGCATTTAACAAGATAGTCCTTAACAACTTCTTAAGGGAAAAAAATAAAAtt                   <  2:127886/140‑1 (MQ=255)                                                                                                                    aTCACCGGTGCGCTGCACAGGACCATTACTGCGATTACTGCTGCGAATTTTTTCATGTTTATTACTCCCTTTAATGTCTGTTTCCGAGCATTTAACAAGATAGTCCTTAACAACTTCTTAAGGGAAAAAAATAAAATTTa                 <  1:691117/140‑1 (MQ=255)                                                                                                                    aTCACCGGTGCGCTGCACAGGACCATTACTGCGATTACTGCTGCGAATTTTTTCATGTTTATTACTCCCTTTAATGTCTGTTTCCGAGCATTTAACAAGATAGTCCTTAACAACTTCTTAAGGGAAAAAAATAAAATTTa                 <  2:165752/140‑1 (MQ=255)                                                                                                                       aCCGGTGCGCTGCACAGGACCATTACTGCGATTACTGCTGCGAATTTTTTCATGTTTATTACTCCCTTTAATGTCTGTTTCCGAGCATTTAACAAGATAGTCCTTAACAACTTCTTAAGGGAAAAAAATAAAATTTAGTg              <  1:1271370/140‑1 (MQ=255)                                                                                                                       aCCGGTGCGCTGCACAGGACCATTACTGCGATTACTGCTGCGAATTTTTTCATGTTTATTACTCCCTTTAATGTCTGTTTCCGAGCATTTAACAAGATAGTCCTTAACAACTTCTTAAGGGAAAAAAATAAAATTTAGTg              <  1:1224504/140‑1 (MQ=255)                                                                                                                        ccGGTGCGCTGCACAGGACCATTACTGCGATTACTGCTGCGAATTTTTTCATGTTTATTACTCCCTTTAATGTCTGTTTCCGAGCATTTAACAAGATAGTCCTTAACAACTTCTTAAGGGAAAAAAATAAAATTTAGTGc             <  1:538630/140‑1 (MQ=255)                                                                                                                            tGCGCTGCACAGGACCATTACTGCGATTACTGCTGCGAATTTTTTCATGTTTATTACTCCCTTTAATGTCTGTTTCCGAGCATTTAACAAGATAGTCCTTAACAACTTCTTAAGGGAAAAAAATAAAAtt                   >  1:145962/1‑130 (MQ=255)                                                                                                                            tGCGCTGCACAGGACCATTACTGCGATTACTGCTGCGAATTTTTTCATGTTTATTACTCCCTTTAATGTCTGTTTCCGAGCATTTAACAAGATAGTCCTTAACAACTTCTTAAGGGAAAAAAATAAAAtt                   <  2:145962/130‑1 (MQ=255)                                                                                                                               gcTGCACAGGACCATTACTGCGATTACTGCTGCGAATTTTTTCATGTTTATTACTCCCTTTAATGTCTGTTTCCGAGCATTTAACAAGATAGTCCTTAACAACTTCTTAAGGGAAAAAAATAAAAtt                   <  2:1126486/127‑1 (MQ=255)                                                                                                                               gcTGCACAGGACCATTACTGCGATTACTGCTGCGAATTTTTTCATGTTTATTACTCCCTTTAATGTCTGTTTCCGAGCATTTAACAAGATAGTCCTTAACAACTTCTTAAGGGAAAAAAATAAAAtt                   >  1:1126486/1‑127 (MQ=255)                                                                                                                               gcTGCACAGGACCATTACTGCGATTACTGCTGCGAATTTTTTCATGTTTATTACTCCCTTTAATGTCTGTTTCCGAGCATTTAACAAGATAGTCCTTAACAACTTCTTAAGGGAAAAAAATAAAATTTAGTGCTGTACag      >  2:511818/1‑140 (MQ=255)                                                                                                                                 tGCACAGGACCATTACTGCGATTACTGCTGCGAATTTTTTCATGTTTATTACTCCCTTTAATGTCTGTTTCCGAGCATTTAACAAGATAGTTCTTAACAACTTCTTAAGGGAAAAAAATAAAATTTAGTGCTGTACagag    >  1:1327498/1‑140 (MQ=255)                                                                                                                                 tGCACAGGACCATTACTGCGATTACTGCTGCGAATTTTTTCATGTTTATTACTCCCTTTAATGTCTGTTTCCGAGCATTTAACAAGATAGTCCTTAACAACTTCTTAAGGGAAAAAAATAAAATTTAGTGCTGTACagag    <  2:399898/140‑1 (MQ=255)                                                                                                                                  gCACAGGACCATTACTGCGATTACTGCTGCGAATTTTTTCATGTTTATTACTCCCTTTAATGTCTGTTTCCGAGCATTTAACAAGATAGTCCTTAACAACTTCTTAAGGGAAAAAAATAAAATTTAGTGCTGTACAGAgc   <  2:345282/140‑1 (MQ=255)                                                                                                                                   cacaGGACCATTACTGCGATTACTGCTGCGAATTTTTTCATGTTTATTACTCCCTTTAATGTCTGTTTCCGAGCATTTAACAAGATAGTCCTTAACAACTTCTTAAGGGAAAAAAATAAAAtt                   <  1:176039/123‑1 (MQ=255)                                                                                                                                   cacaGGACCATTACTGCGATTACTGCTGCGAATTTTTTCATGTTTATTACTCCCTTTAATGTCTGTTTCCGAGCATTTAACAAGATAGTCCTTAACAACTTCTTAAGGGAAAAAAATAAAAtt                   >  2:176039/1‑123 (MQ=255)                                                                                                                                   cacaGGACCATTACTGCGATTACTGCTGCGAATTTTTTCATGTTTATTACTCCCTTTAATGTCTGTTTCCGAGCATTTAACAAGATAGTCCTTAACAACTTCTTAAGGGAAAAAAATAAAATTTAGTGCTGTACAGAgcg  <  2:116475/140‑1 (MQ=255)                                                                                                                                   cacaGGACCATTACTGCGATTACTGCTGCGAATTTTTTCATGTTTATTACTCCCTTTAATGTCTGTTTCCGAGCATTTAACAAGATAGTCCTTAACAACTTCTTAAGGGAAAAAAATAAAATTTAGTGCTGTACAGAgcg  <  1:233127/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                      GTCACGCAGGGATTTTGCGCTTTCTACAGTCGTTACGCTGCCGTTCGGCCCCTGGAATCCTCCGGCCTGACTTTGCGTTGCCGATGGGCCAGAAAAACCGCCCTGCTCTGCTGCCATCACCGGTGCGCTGCACAGGGCCATTACTGCGATTACTGCTGCGAATTTTTTCATGTTTATTACTCCCTTTAATGTCTGTTTCCGAGCATTTAACAAGATAGTCCTTAACAACTTCTTAAGGGAAAAAAATAAAATTTAGTGCTGTACAGAGCG  >  NC_000913/3169506‑3169775

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑