Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,229,122	G→A	V79I (GTT→ATT)	ygjK →	glycoside hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,229,122	0	G	A	98.7%	268.4 / ‑4.7	78	V79I (GTT→ATT)	ygjK	glycoside hydrolase
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (37/40);  total (37/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

CCGTACTGGCGCGCCGCAGTACATGAAGGATTACGATTACGACGATCACCAGCGTTTTAATCCGTTTTTCGATCTCGGAGCCTGGCATGGTCATCTGTTGCCAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCGTTGCGCTGCTGACGGAAGAGTACATCAACTTTATGGCCAGCAATTTCGACCGCCTGACCGTCTGGCAGGACGGCAAGAAAGTCGACTTTACGCTGGAGGCATACAGTATTCCCGGTGCGCTGGTGCAAAAACTGAC  >  NC_000913/3228986‑3229258                                                                                                                                         |                                                                                                                                         ccGTACTGGCGCGCCGCAGTACATGAAGGATTACGATTACGACGATCACCAGCGTTTTAATCCGTTTTTCGATCTCGGAGCCTGGCATGGTCATCTGTTGCCAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCATTg                                                                                                                                       >  2:442497/1‑140 (MQ=255)  cGTACTGGCGCGCCGCAGTACATG‑AGGATTACGATTACGACGATCACCAGCGTTTTAATCCGTTTTTCGATCTCGGAGCCTGGCATGGTCATCTGTTGCCAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCATTgcg                                                                                                                                     <  2:1298375/140‑1 (MQ=255)  cGTACTGGCGCGCCACAGTACATGAAGGATTACGATTACGACGATCACCAGCGTTTTAATCCGTTTTTCGATCTCGGAGCCTGGCATGGTCATCTGTTGCCACACGGCCCTAACACCATGGGCGGCTTTCCGGGCATTgc                                                                                                                                      <  2:1247770/140‑1 (MQ=255)   gTACTGGCGCGCCGCAGTACATGAAGGATTACGATTACGACGATCACCAGCGTTTTAATCCGTTTTTCGATCTCGGAGCCTGGCATGGTCATCTGTTGCCAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCATTgcg                                                                                                                                     <  2:575523/140‑1 (MQ=255)   gTACTGGCGCGCCGCAGTACATGAAGGATTACGATTACGACGATCACCAGCGTTTTAATCCGTTTTTCGATCTCGGAGCCTGGCATGGTCATCTGTTGCCAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCATTgcg                                                                                                                                     <  1:385500/140‑1 (MQ=255)    tACTGGCGCGCCGCAGTACATGAAGGATTACGATTACGACGATCACCAGCGTTTTAATCCGTTTTTCGATCTCGGAGCCTGGCATGGTCATCTGTTGCCAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCATTgcgc                                                                                                                                    >  1:1059262/1‑140 (MQ=255)     aCTGGCGCGCCGCAGTACATGAAGGATTACGATTACGACGATCACCAGCGTTTTAATCCGTTTTTCGATCTCGGAGCCTGGCATGGTCATCTGTTGCCAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCATTGCgct                                                                                                                                   <  2:1210186/140‑1 (MQ=255)      cTGGCGCGCCGCAGTACATGAAGGATTACGATTACGACGATCACCAGCGTTTTAATCCGTTTTTCGATCTCGGAGCCTGGCATGGTCATCTGTTGCCAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCATTGCgctg                                                                                                                                  >  2:855398/1‑140 (MQ=255)        ggCGCGCCGCAGTACATGAAGGATTACGATTACGACGATCACCAGCGTTTTAATCCGTTTTTCGATCTCGGAGCCTGGCATGGTCATCTGTTGCCAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCATTGCgctgct                                                                                                                                <  2:986769/140‑1 (MQ=255)          cgcgcCGCAGTACATGAAGGATTACGATTACGACGATCACCAGCGTTTTAATCCGTTTTTCGATCTCGGAGCCTGGCATGGTCATCTGTTGCCAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCATTGCGCTGCTGa                                                                                                                              >  2:246168/1‑140 (MQ=255)          cgcgcCGCAGTACATGAAGGATTACGATTACGACGATCACCAGCGTTTTAATCCGTTTTTCGATCTCGGAGCCTGGCATGGTCATCTGTTGCCAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCATTGCGCTGCTGa                                                                                                                              <  1:41696/140‑1 (MQ=255)          cgcgcCGCAGTACATGAAGGATTACGATTACGACGATCACCAGCGTTTTAATCCGTTTTTCGATCTCGGAGCCTGGCATGGTCATCTGTTGCCAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCATTGCGCTGCTGa                                                                                                                              >  2:301222/1‑140 (MQ=255)             gccgcAGTACATGAAGGATTACGATTACGACGATCACCAGCGTTTTAATCCGTTTTTCGATCTCGGAGCCTGGCATGGTCATCTGTTGCCAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCATTGCGCTGCTGACgg                                                                                                                           <  2:1161686/140‑1 (MQ=255)             gccgcAGTACATGAAGGATTACGATTACGACGATCACCAGCGTTTTAATCCGTTTTTCGATCTCGGAGCCTGGCATGGTCATCTGTTGCCAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCATTGCGCTGCTGACgg                                                                                                                           <  1:90418/140‑1 (MQ=255)              ccgcAGTACATGAAGGATTACGATTACGACGATCACCAGCGTTTTAATCCGTTTTTCGATCTCGGAGCCTGGCATGGTCATCTGTTGCCAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCATTGCGCTGCTGACGGa                                                                                                                          >  2:197414/1‑140 (MQ=255)                gcAGTACATGAAGGATTACGATTACGACGATCACCAGCGTTTTAATCCGTTTTTCGATCTCGGAGCCTGGCATGGTCATCTGTTGCCAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCATTGCGCTGCTGACGGAag                                                                                                                        <  1:1358546/140‑1 (MQ=255)                gcAGTACATGAAGGATTACGATTACGACGATCACCAGCGTTTTAATCCGTTTTTCGATCTCGGAGCCTGGCATGGTCATCTGTTGCCAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCATTGCGCTGCTGACGGAag                                                                                                                        <  1:657607/140‑1 (MQ=255)                gcAGTACATGAAGGATTACGATTACGACGATCACCAGCGTTTTAATCCGTTTTTCGATCTCGGAGCCTGGCATGGTCATCTGTTGCCAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCATTGCGCTGCTGACGGAag                                                                                                                        <  1:454103/140‑1 (MQ=255)                         gAAGGATTACGATTACGACGATCACCAGCGTTTTAATCCGTTTTTCGATCTCGGAGCCTGGCATGGTCATCTGTTGCCAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCATTGCGCTGCTGACGGAAGAGTACATCa                                                                                                               <  1:197414/140‑1 (MQ=255)                         gAAGGATTACGATTACGACGATCACCAGCGTTTTAATCCGTTTTTCGATCTCGGAGCCTGGCATGGTCATCTGTTGCCAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCATTGCGCTGCTGACGGAAGAGTACATCa                                                                                                               >  1:107713/1‑140 (MQ=255)                         gAAGGATTACGATTACGACGATCACCAGCGTTTTAATCCGTTTTTCGATCTCGGAGCCTGGCATGGTCATCTGTTGCCAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCATTGCGCTGCTGACGGAAGAGTACATCa                                                                                                               <  2:931895/140‑1 (MQ=255)                         gAAGGATTACGATTACGACGATCACCAGCGTTTTAATCCGTTTTTCGATCTCGGAGCCTGGCATGGTCATCTGTTGCCAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCATTGCGCTGCTGACGGAAGAGTACATCa                                                                                                               <  2:944370/140‑1 (MQ=255)                         gAAGGATTACGATTACGACGATCACCAGCGTTTTAATCCGTTTTTCGATCTCGGAGCCTGGCATGGTCATCTGTTGCCAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCATTGCGCTGCTGACGGAAGAGTACATCa                                                                                                               >  1:1244497/1‑140 (MQ=255)                              attacgattacgACGATCACCAGCGTTTTAATCCGTTTTTCGATCTCGGAGCCTGGCATGGTCATCTGTTGCCAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCATTGCGCTGCTGACGGAAGAGTACATCAACttt                                                                                                          >  2:226104/1‑140 (MQ=255)                              attacgattacgACGATCACCAGCGTTTTAATCCGTTTTTCGATCTCGGAGCCTGGCATGGTCATCTGTTGCCAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCATTGCGCTGCTGACGGAAGAGTACATCAACttt                                                                                                          >  1:72275/1‑140 (MQ=255)                                tacgattacgaCGATCACCAGCGTTTTAATCCGTTTTTCGATCTCGGAGCCTGGCATGGTCATCTGTTGCCAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCATTGCGCTGCTGACGGAAGAGTACATCAACTTTAt                                                                                                        >  1:938138/1‑140 (MQ=255)                                tacgattacgaCGATCACCAGCGTTTTAATCCGTTTTTCGATCTCGGAGCCTGGCATGGTCATCTGTTGCCAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCATTGCGCTGCTGACGGAAGAGTACATCAACTTTAt                                                                                                        >  2:642861/1‑140 (MQ=255)                                 acgattacgaCGATCACCAGCGTTTTAATCCGTTTTTCGATCTCGGAGCCTGGCATGGTCATCTGTTGCCAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCATTGCGCTGCTGACGGAAGAGTACATCAACTTTATg                                                                                                       >  2:876298/1‑140 (MQ=255)                                  cgattacgaCGATCACCAGCGTTTTAATCCGTTTTTCGATCTCGGAGCCTGGCATGGTCATCTGTTGCCAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCATTGCGCTGCTGACGGAAGAGTACATCAACTTTATgg                                                                                                      >  1:164257/1‑140 (MQ=255)                                   gattacgaCGATCACCAGCGTTTTAATCCGTTTTTCGATCTCGGAGCCTGGCATGGTCATCTGTTGCCAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCATTGCGCTGCTGACGGAAGAGTACATCAACTTTATGGc                                                                                                     <  1:272848/140‑1 (MQ=255)                                             aTCACCAGCGTTTTAATCCGTTTTTCGATCTCGGAGCCTGGCATGGTCATCTGTTGCCAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCATTGCGCTGCTGACGGAAGAGTACATCAACTTTATGGCCAGCAATTTc                                                                                           >  1:147105/1‑140 (MQ=255)                                             aTCACCAGCGTTTTAATCCGTTTTTCGATCTCGGAGCCTGGCATGGTCATCTGTTGCCAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCATTGCGCTGCTGACGGAAGAGTACATCAACTTTATGGCCAGCAATTTc                                                                                           >  1:563437/1‑140 (MQ=255)                                             aTCACCAGCGTTTTAATCCGTTTTTCGATCTCGGAGCCTGGCATGGTCATCTGTTGCCAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCATTGCGCTGCTGACGGAAGAGTACATCAACTTTATGGCCAGCAATTTc                                                                                           >  2:207847/1‑140 (MQ=255)                                              tCACCAGCGTTTTAATCCGTTTTTCGATCTCGGAGCCTGGCATGGTCATCTGTTGCCAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCATTGCGCTGCTGACGGAAGAGTACATCAACTTTATGGCCAGCAATTTCg                                                                                          >  1:659676/1‑140 (MQ=255)                                                aCCAGCGTTTTAATCCGTTTTTCGATCTCGGAGCCTGGCATGGTCATCTGTTGCCAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCATTGCGCTGCTGACGGAAGAGTACATCAACTTTATGGCCAGCAATTTCGAc                                                                                        >  2:152889/1‑140 (MQ=255)                                                       ttttAATCCGTTTTTCGATCTCGGAGCCTGGCATGGTCATCTGTTGCCAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCATTGCGCTGCTGACGGAAGAGTACATCAACTTTATGGCCAGCAATTTCGACCGCCTGa                                                                                 <  2:1059262/140‑1 (MQ=255)                                                       ttttAATCCGTTTTTCGATCTCGGAGCCTGGCATGGTCATCTGTTGCCAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCATTGCGCTGCTGACGGAAGAGTACATCAACTTTATGGCCAGCAATTTCGACCGCCTGa                                                                                 >  1:1295928/1‑140 (MQ=255)                                                         ttAATCCGTTTTTCGATCTCGGAGCCTGGCATGGTCATCTGTTGCCAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCATTGCGCTGCTGACGGAAGAGTACATCAACTTTATGGCCAGCAATTTCGACCGCCTGAcc                                                                               >  2:104500/1‑140 (MQ=255)                                                         ttAATCCGTTTTTCGATCTCGGAGCCTGGCATGGCCATCTGTTGCCAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCATTGCGCTGCTGACGGAAGAGTACATCAACTTTATGGCCAGCAATTTCGACCGCCTGAcc                                                                               >  2:1016962/1‑140 (MQ=255)                                                               cGTTTTTCGATCTCGGAGCCTGGCATGGTCATCTGTTGCCAGACGGCCCTAACGCCATGGGCGGCTTTCCGGGCATTGCGCTGCTGACGGAAGAGTACATCAACTTTATGGCCAGCAATTTCGACCGCCTGACCGTCTgg                                                                         >  2:248595/1‑140 (MQ=255)                                                                         tctcGGAGCCTGGCATGGTCATCTGTTGCCAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCATTGCGCTGCTGACGGAAGAGTACATCAACTTTATGGCCAGCAATTTCGACCGCCTGACCGTCTGGCAGGACGGCa                                                               >  2:619403/1‑140 (MQ=255)                                                                         tctcGGAGCCTGGCATGGTCATCTGTTGCCAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCATTGCGCTGCTGACGGAAGAGTACATCAACTTTATGGCCAGCAATTTCGACCGCCTGACCGTCTGGCAGGACGGCa                                                               >  2:548271/1‑140 (MQ=255)                                                                         tctcGGAGCCTGGCATGGTCATCTGTTGCCAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCATTGCGCTGCTGACGGAAGAGTACATCAACTTTATGGCCAGCAATTTCGACCGCCTGACCGTCTGGCAGGACGGCa                                                               >  2:1063148/1‑140 (MQ=255)                                                                                gCCTGGCATGGTCATCTGTTGCCAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCATTGCGCTGCTGACGGAAGAGTACATCAACTTTATGGCCAGCAATTTCGCCCGCCTGACCGTCTGGCAGGACGGCAAGAAAGt                                                        <  1:1260467/140‑1 (MQ=255)                                                                                           tCATCTGTTGCCAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCATTGCGCTGCTGACGGAAGAGTACATCAACTTTATGGCCAGCAATTTCGACCGCCTGACCGTCTGGCAGGACGGCAAGAAAGTCGACTTTAc                                               >  1:205436/1‑138 (MQ=255)                                                                                           tCATCTGTTGCCAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCATTGCGCTGCTGACGGAAGAGTACATCAACTTTATGGCCAGCAATTTCGACCGCCTGACCGTCTGGCAGGACGGCAAGAAAGTCGACTTTAc                                               <  2:205436/138‑1 (MQ=255)                                                                                           tCATCTGTTGCCAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCATTGCGCTGCTGACGGAAGAGTACATCAACTTTATGGCCAGCAATTTCGACCGCCTGACCGTCTGGCAGGACGGCAAGAAAGTCGACTTTACGc                                             >  1:515551/1‑140 (MQ=255)                                                                                           tCATCTGTTGCCAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCATTGCGCTGCTGACGGAAGAGTACATCAACTTTATGGCCAGCAATTTCGACCGCCTGACCGTCTGGCAGGACGGCAAGAAAGTCGACTTTACGc                                             >  1:12749/1‑140 (MQ=255)                                                                                           tCATCTGTTGCCAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCATTGCGCTGCTGACGGAAGAGTACATCAACTTTATGGCCAGCAATTTCGACCGCCTGACCGTCTGGCAGGACGGCAAGAAAGTCGACTTTACGc                                             >  2:416581/1‑140 (MQ=255)                                                                                             aTCTGTTGCCAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCATTGCGCTGCTGACGGAAGAGTACATCAACTTTATGGCCAGCAATTTCGACCGCCTGACCGTCTGGCAGGACGGCAAGAAAGTCGACTTTACGCTg                                           <  2:963293/140‑1 (MQ=255)                                                                                             aTCTGTTGCCAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCATTGCGCTGCTGACGGAAGAGTACATCAACTTTATGGCCAGCAATTTCGACCGCCTGACCGTCTGGCAGGACGGCAAGAAAGTCGACTTTACGCTg                                           <  2:164257/140‑1 (MQ=255)                                                                                              tCTGTTGCCAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCGTTGCGCTGCTGACGGAAGAGTACATCACCTTTATGGCCAGCAATTTCGACCGCCTGACCGTCTTGCCGGACGGCAAGAAAGTCGACTTTACGCTgg                                          >  2:1323009/1‑140 (MQ=255)                                                                                              tCTGTTGCCAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCATTGCGCTGCTGACGGAAGAGTACATCAACTTTATGGCCAGCAATTTCGACCGCCTGACCGTCTGGCAGGACGGCAAGAAAGTCGACTTTACGCTgg                                          <  2:579013/140‑1 (MQ=255)                                                                                              tCTGTTGCCAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCATTGCGCTGCTGACGGAAGAGTACATCAACTTTATGGCCAGCAATTTCGACCGCCTGACCGTCTGGCAGGACGGCAAGAAAGTCGACTTTACGCTgg                                          <  2:206319/140‑1 (MQ=255)                                                                                               cTGTTGCCAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCATTGCGCTGCTGACGGAAGAGTACATCAACTTTATGGCCAGCAATTTCGACCGCCTGACCGTCTGGCAGGACGGCAAGAAAGTCGACTTTACGCTGGa                                         >  2:88191/1‑140 (MQ=255)                                                                                               cTGTTGCCAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCATTGCGCTGCTGACGGAAGAGTACATCAACTTTATGGCCAGCAATTTCGACCGCCTGACCGTCTGGCAGGACGGCAAGAAAGTCGACTTTACGCTGGa                                         <  1:104500/140‑1 (MQ=255)                                                                                                tGTTGCCAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCATTGCGCTGCTGACGGAAGAGTACATCAACTTTATGGCCAGCAATTTCGACCGCCTGACCGTCTGGCAGGACGGCAAGAAAGTCGACTTTACGCTGGAg                                        <  1:152889/140‑1 (MQ=255)                                                                                                tGTTGCCAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCATTGCGCTGCTGACGGAAGAGTACATCAACTTTATGGCCAGCAATTTCGACCGCCTGACCGTCTGGCAGGACGGCAAGAAAGTCGACTTTACGCTGGAg                                        <  2:563437/140‑1 (MQ=255)                                                                                                   tGCCAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCATTGCGCTGCTGACGGAAGAGTACATCAACTTTATGGCCAGCAATTTCGACCGCCTGACCGTCTGGCAGGACGGCAAGAAAGTCGACTTTACGCTGGAGGCa                                     <  1:1337716/140‑1 (MQ=255)                                                                                                     ccAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCATTGCGCTGCTGACGGAAGAGTACATCAACTTTATGGCCAGCAATTTCGACCGCCTGACCGTCTGGCAGGACGGCAAGAAAGTCGACTTTACGCTGGAGGCATa                                   >  2:399676/1‑140 (MQ=255)                                                                                                      cAGACGGCCCTAACGCCATGGGCGGCTTTCCGGGCATTGCGCTGCTGACGGAAGAGTACATCAACTTTATGGCCAGCAATTTCGACCGCCTGACCGTCTGGCAGGACGGCAAGAAAGTCGACTTTACGCTGGAGGCATAc                                  <  1:248595/140‑1 (MQ=255)                                                                                                      cAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCATTGCGCTGCTGACGGAAGAGTACATCAACTTTATGGCCAGCAATTTCGACCGCCTGACCGTCTGGCAGGACGGCAAGAAAGTCGACTTTACGCTGGAGGCATAc                                  <  1:790411/140‑1 (MQ=255)                                                                                                      cAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCATTGCGCTGCTGACGGAAGAGTACATCAACTTTATGGCCAGCAATTTCGACCGCCTGACCGTCTGGCAGGACGGCAAGAAAGTCGACTTTACGCTGGAGGCATAc                                  >  2:279970/1‑140 (MQ=255)                                                                                                        gACGGCCCTAACACCATGGGCGGCTTTCCGGGCATTGCGCTGCTGACGGAAGAGTACATCAACTTTATGTCCAGCAATTTCGACCGCCTGACCGTCTGGCAGGACGGCAAGAAAGTCGACTTTACGCTGGAGGCATACAg                                <  2:72275/140‑1 (MQ=255)                                                                                                            gCCCTAACACCATGGGCGGCTTTCCGGGCATTGCGCTGCTGACGGAAGAGTACATCAACTTTATGGCCAGCAATTTCGACCGCCTGACCGTCTGGCAGGACGGCAAGAAAGTCGACTTTACGCTGGAGGCATACAGTAtt                            >  1:805932/1‑140 (MQ=255)                                                                                                            gCCCTAACACCATGGGCGGCTTTCCGGGCATTGCGCTGCTGACGGAAGAGTACATCAACTTTATGGCCAGCAATTTCGACCGCCTGACCGTCTGGCAGGACGGCAAGAAAGTCGACTTTACGCTGGAGGCATACAGTAtt                            >  2:604022/1‑140 (MQ=255)                                                                                                                       aTGGGCGGCTTTCCGGGCATTGCGCTGCTGACGGAAGAGTACATCAACTTTATGGCCAGCAATTTCGACCGCCTGGCCGCCTGGCAGGACGGCAAGAAAGTCGACTTTACGCTGGAGGCATACAGTATTCCCGGTGCGCt                 >  1:1259700/1‑140 (MQ=255)                                                                                                                       aTGGGCGGCTTTCCGGGCATTGCGCTGCTGACGGAAGAGTACATCAACTTTATGGCCAGCAATTTCGACCGCCTGACCGTCTGGCAGGACGGCAAGAAAGTCGACTTTACGCTGGAGGCATACAGTATTCCCGGTGCGCt                 <  2:147105/140‑1 (MQ=255)                                                                                                                          ggcggcTTTCCGGGCATTGCGCTGCTGACGGAAGAGTACATCAACTTTATGGCCAGCAATTTCGACCGCCTGACCGTCTGGCAGGACGGCAAGAAAGTCGACTTTACGCTGGAGGCATACAGTATTCCCGGTGCGCTGGt              >  1:664116/1‑140 (MQ=255)                                                                                                                           gcggcTTTCCGGGCATTGCGCTGCTGACGGAAGAGTACATCAACTTTATGGCCAGCAATTTCGACCGCCTGACCGTCTGGCAGGACGGCAAGAAAGTCGACTTTACGCTGGAGGCATACAGTATTCCCGGTGCGCTGGTg             <  2:147605/140‑1 (MQ=255)                                                                                                                           gcggcTTTCCGGGCATTGCGCTGCTGACGGAAGAGTACATCAACTTTATGGCCAGCAATTTCGACCGCCTGACCGTCTGGCAGGACGGCAAGAAAGTCGACTTTACGCTGGAGGCATACAGTATTCCCGGTGCGCTGGTg             <  1:604022/140‑1 (MQ=255)                                                                                                                                 ttCCGGGCATTGCGCTGCTGACGGAAGAGTACATCAACTTTATGGCCAGCAATTTCGACCGCCTGACCGTCTGGCAGGACGGCAAGAAAGTCGACTTTACGCTGGAGGCATACAGTATTCCCGGTGCGCTGGTGCaaaaa       <  1:1063148/140‑1 (MQ=255)                                                                                                                                 ttCCGGGCATTGCGCTGCTGACGGAAGAGTACATCAACTTTATGGCCAGCAATTTCGACCGCCTGACCGTCTGGCAGGACGGCAAGAAAGTCGACTTTACGCTGGAGGCATACAGTATTCCCGGTGCGCTGGTGCaaaaa       <  1:548271/140‑1 (MQ=255)                                                                                                                                    cGGGCATTGCGCTGCTGACGGAAGAGTACATCAACTTTATGGCCAGCAATTTCGACCGCCTGACCGTCTGGCAGGACGGCAAGAAAGTCGACTTTATGCTGGAGGCATACAGTATTCCCGGTGCGCTGGTGCAAAAACTg    <  1:207847/140‑1 (MQ=255)                                                                                                                                    cGGGCATTGCGCTGCTGACGGAAGAGTACATCAACTTTATGGCCAGCAATTTCGACCGCCTGACCGTCTGGCAGGACGGCAAGAAAGTCGACTTTACGCTGGAGGCATACAGTATTCCCGGTGCGCTGGTGCAAAAACTg    <  2:515551/140‑1 (MQ=255)                                                                                                                                    cGGGCATTGCGCTGCTGACGGAAGAGTACATCAACTTTATGGCCAGCAATTTCGACCGCCTGACCGTCTGGCAGGACGGCAAGAAAGTCGACTTTACGCTGGAGGCATACAGTATTCCCGGTGCGCTGGTGCAAAAACTg    <  1:88191/140‑1 (MQ=255)                                                                                                                                    cGGGCATTGCGCTGCTGACGGAAGAGTACATCAACTTTATGGCCAGCAATTTCGACCGCCTGACCGTCTGGCAGGACGGCAAGAAAGTCGACTTTACGCTGGAGGCATACAGTATTCCCGGTGCGCTGGTGCAAAAACTg    <  1:619403/140‑1 (MQ=255)                                                                                                                                    cGGGCATTGCGCTGCTGACGGAAGAGTACATCAACTTTATGGCCAGCAATTTCGACCGCCTGACCGTCTGGCAGGACGGCAAGAAAGTCGACTTTACGCTGGAGGCATACAGTATTCCCGGTGCGCTGGTGCAAAAACTg    <  2:949822/140‑1 (MQ=255)                                                                                                                                     gggCATTGCGCTGCTGACGGAAGAGTACATCAACTTTATGGCCAGCAATTTCGACCGCCTGACCGTCTGGCAGGACGGCAAGAAAGTCGACTTTACGCTGGAGGCATACAGTATTCCCGGTGCGCTGGTGCAAAAACTGa   <  1:1063014/140‑1 (MQ=255)                                                                                                                                      ggCGTTGCGCTGCTGACGGAAGAGTACATCAACTTTATGGCCAGCAATTTCGACCGCCTGACCGTCTGGCAGGACGGCAAGAAAGTCGACTTTACGCTGGAGGCATTCAGTATTCCCGGTGCGCTGGTGCAAAAACTGAc  <  1:1323009/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                         CCGTACTGGCGCGCCGCAGTACATGAAGGATTACGATTACGACGATCACCAGCGTTTTAATCCGTTTTTCGATCTCGGAGCCTGGCATGGTCATCTGTTGCCAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCGTTGCGCTGCTGACGGAAGAGTACATCAACTTTATGGCCAGCAATTTCGACCGCCTGACCGTCTGGCAGGACGGCAAGAAAGTCGACTTTACGCTGGAGGCATACAGTATTCCCGGTGCGCTGGTGCAAAAACTGAC  >  NC_000913/3228986‑3229258

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑