Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 437,999 C→T R46H (CGT→CAT)  yajO ← 1‑deoxyxylulose‑5‑phosphate synthase YajO

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913437,9990CT100.0% 154.3 / NA 44R46H (CGT→CAT) yajO1‑deoxyxylulose‑5‑phosphate synthase YajO
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (17/27);  total (17/27)

ATGGAACACTTTGGTCGCAACGACCACGTCTTCACGACGGGCGAAATCCCGCAGTGCGCGACCGACGATCTCTTCGCTGCTGCCGTCAGAATAACTGTTGGCGGTATCAAAGAAATTTATGCCGCCTTCCAGTGCACGTTTAATTATGGGACGGCTGCTTTCTTCCGGCAGTGTCCATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGGAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCA  >  NC_000913/437863‑438134
                                                                                                                                        |                                                                                                                                       
aTGGAACACTTTGGTCGCAACGACCACGTCTTCACGACGGGCGAAATCCCGCAGTGCGCGACCGACGATCTCTTCGCTGCTGCCGTCAGAATAACTGTTGGCGGTATCAAAGAAATTTATGCCGCCTTCCAGTGCATGtt                                                                                                                                      <  2:1174786/140‑1 (MQ=255)
aTGGAACACTTTGGTCGCAACGACCACGTCTTCACGACGGGCGAAATCCCGCAGTGCGCGACCGACGATCTCTTCGCTGCTGCCGTCAGAATAACTGTTGGCGGTATCAAAGAAATTTATGCCGCCTTCCAGTGCATGtt                                                                                                                                      <  2:203786/140‑1 (MQ=255)
           tGGTCGCAACGACCACGTCTTCACGACGGGCGAAATCCCGCAGTGCGCGACCGACGATCTCTTCGCTGCTGCCGTCAGAATAACTGTTGGCGGTATCAAAGAAATTTATGTCGCCTTCCAGTGCATGTTTAATTATGGGa                                                                                                                           >  1:1040995/1‑140 (MQ=255)
           tGGTCGCAACGACCACGTCTTCACGACGGGCGAAATCCCGCAGTGCGCGACCGACGATCTCTTCGCTGCTGCCGTCAGAATAACTGTTGGCGGTATCAAAGAAATTTATGCCGCCTTCCAGTGCATGTTTAATTATGGGa                                                                                                                           >  2:1263765/1‑140 (MQ=255)
           tGGTCGCAACGACCACGTCTTCACGACGGGCGAAATCCCGCAGTGCGCGACCGACGATCTCTTCGCTGCTGCCGTCAGAATAACTGTTGGCGGTATCAAAGAAATTTATGCCGCCTTCCAGTGCATGTTTAATTATGGGa                                                                                                                           >  1:486353/1‑140 (MQ=255)
              tCGCAACGACCACGTCTTCACGACGGGCGAAATCCCGCAGTGCGCGACCGACGATCTCTTCGCTGCTGCCGTCAGAATAACTGTTGGCGGTATCAAAGAAATTTATGCCGCCTTCCAGTGCATGTTTAATTATGGGACgg                                                                                                                        <  2:789251/140‑1 (MQ=255)
                 cAACGACCACGTCTTCACGACGGGCGAAATCCCGCAGTGCGCGACCGACGATCTCTTCGCTGCTGCCGTCAGAATAACTGTTGGCGGTATCAAAGAAATTTATGCCGCCTTCCAGTGCATGTTTAATTATGGGACGgctg                                                                                                                     >  1:737841/1‑140 (MQ=255)
                    cGACCACGTCTTCACGACGGGCGAAATCCCGCAGTGCGCGACCGACGATCTCTTCGCTGCTGCCGTCAGAATAACTGTTGGCGGTATCAAAGAAATTTATGCCGCCTTCCAGTGCATGTTTAATTATGGGACGGCTGCtt                                                                                                                  <  2:486353/140‑1 (MQ=255)
                            tCTTCACGACGGGCGAAATCCCGCAGTGCGCGACCGACGATCTCTTCGCTGCTGCCGTCAGAATAACTGTTGGCGGTATCAAAGAAATTTATGCCGCCTTCCAGTGCATGTTTAATTATGGGACGGCTGCTTTCTTCCgg                                                                                                          <  2:484923/140‑1 (MQ=255)
                            tCTTCACGACGGGCGAAATCCCGCAGTGCGCGACCGACGATCTCTTCGCTGCTGCCGTCAGAATAACTGTTGGCGGTATCAAAGAAATTTATGCCGCCTTCCAGTGCATGTTTAATTATGGGACGGCTGCTTTCTTCCgg                                                                                                          <  2:998095/140‑1 (MQ=255)
                              ttCACGACGGGCGAAATCCCGCAGTGCGCGACCGACGATCTCTTCGCTGCTGCCGTCAGAATAACTGTTGGCGGTATCAAAGAAATTTATGCCGCCTTCCAGTGCATGTTTAATTATGGGACGGCTGCTTTCTTCCGGCa                                                                                                        <  2:175834/140‑1 (MQ=255)
                              ttCACGACGGGCGAAATCCCGCAGTGCGCGACCGACGATCTCTTCGCTGCTGCCGTCAGAATAACTGTTGGCGGTATCAAAGAAATTTATGCCGCCTTCCAGTGCATGTTTAATTATGGGACGGCTGCTTTCTTCCGGCa                                                                                                        <  2:126959/140‑1 (MQ=255)
                                   gacgGGCGAAATCCCGCAGTGCGCGACCGACGATCTCTTCGCTGCTGCCGTCAGAATAACTGTTGGCGGTATCAAAGAAATTTATGCCGCCTTCCAGTGCATGTTTAATTATGGGACGGCTGCTTTCTTCCGGCAGTGTc                                                                                                   <  2:727028/140‑1 (MQ=255)
                                     cgGGCGAAATCCCGCAGTGCGCGACCGACGATCTCTTCGCTGCTGCCGTCAGAATAACTGTTGGCGGTATCAAAGAAATTTATGCCGCCTTCCAGTGCATGTTTAATTATGGGACGGCTGCTTTCTTCCGGCAGTGTCCa                                                                                                 >  1:258764/1‑140 (MQ=255)
                                     cgGGCGAAATCCCGCAGTGCGCGACCGACGATCTCTTCGCTGCTGCCGTCAGAATAACTGTTGGCGGTATCAAAGAAATTTATGCCGCCTTCCAGTGCATGTTTAATTATGGGACGGCTGCTTTCTTCCGGCAGTGTCCa                                                                                                 <  2:953429/140‑1 (MQ=255)
                                      gggCGAAATCCCGCAGTGCGCGACCGACGATCTCTTCGCTGCTGCCGTCAGAATAACTGTTGGCGGTATCAAAGAAATTTATGCCGCCTTCCAGTGCATGTTTAATTATGGGACGGCTGCTTTCTTCCGGCAGTGTCCAt                                                                                                >  2:899653/1‑140 (MQ=255)
                                       ggCGAAATCCCGCAGTGCGCGACCGACGATCTCTTCGCTGCTGCCGTCAGAATAACTGTTGGCGGTATCAAAGAAATTTATGCCGCCTTCCAGTGCATGTTTAATTATGGGACGGCTGCTTTCTTCCGGCAGTGTCCATg                                                                                               <  2:1217979/140‑1 (MQ=255)
                                             aTCCCGCAGTGCGCGACCGACGATCTCTTCGCTGCTGCCGTCAGAATAACTGTTGGCGGTATCAAAGAAATTTATGCCGCCTTCCAGTGCATGTTTAATTATGGGACGGCTGCTTTCTTCCGGCAGTGTCCATGCGTGAt                                                                                         <  2:739737/140‑1 (MQ=255)
                                                      tGCGCGACCGACGATCTCTTCGCTGCTGCCGTCAGAATAACTGTTGGCGGTATCAAAGAAATTTATGCCGCCTTCCAGTGCATGTTTAATTATGGGACGGCTGCTTTCTTCCGGCAGTGTCCATGCGTGATTACCGCGAt                                                                                <  2:251808/140‑1 (MQ=255)
                                                      tGCGCGACCGACGATCTCTTCGCTGCTGCCGTCAGAATAACTGTTGGCGGTATCAAAGAAATTTATGCCGCCTTCCAGTGCATGTTTAATTATGGGACGGCTGCTTTCTTCCGGCAGTGTCCATGCGTGATTACCGCGAt                                                                                <  1:414730/140‑1 (MQ=255)
                                                        cgcgACCGACGATCTCTTCGCTGCTGCCGTCAGAATAACTGTTGGCGGTATCAAAGAAATTTATGCCGCCTTCCAGTGCATGTTTAATTATGGGACGGCTGCTTTCTTCCGGCAGTGTCCATGCGTGATTACCGCGATCt                                                                              >  2:656135/1‑140 (MQ=255)
                                                        cgcgACCGACGATCTCTTCGCTGCTGCCGTCAGAATAACTGTTGGCGGTATCAAAGAAATTTATGCCGCCTTCCAGTGCATGTTTAATTATGGGACGGCTGCTTTCTTCCGGCAGTGTCCATGCGTGATTACCGCGATCt                                                                              >  2:1393697/1‑140 (MQ=255)
                                                             ccgacGATCTCTTCGCTGCTGCCGTCAGAATAACTGTTGGCGGTATCAAAGAAATTTATGCCGCCTTCCAGTGCATGTTTAATTATGGGACGGCTGCTTTCTTCCGGCAGTGTCCATGCGTGATTACCGCGATCTGGCTc                                                                         >  2:178850/1‑140 (MQ=255)
                                                                acgaTCTCTTCGCTGCTGCCGTCAGAATAACTGTTGGCGGTATCAAAGAAATTTATGCCGCCTTCCAGTGCATGTTTAATTATGGGACGGCTGCTTTCTTCCGGCAGTGTCCATGCGTGATTACCGCGATCTGGCTCGcc                                                                      >  2:381421/1‑140 (MQ=255)
                                                                    tctcTTCGCTGCTGCCGTCAGAATAACTGTTGGCGGTATCAAAGAAATTTATGCCGCCTTCCAGTGCATGTTTAATTATGGGACGGCTGCTTTCTTCCGGCAGTGTCCATGCGTGATTACCGCGATCTGGCTCGCCAAAg                                                                  <  1:616834/140‑1 (MQ=255)
                                                                       ctTCGCTGCTGCCGTCAGAATAACTGTTGGCGGTATCAAAGAAATTTATGCCGCCTTCCAGTGCATGTTTAATTATGGGACGGCTGCTTTCTTCCGGCAGTGTCCATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTc                                                               >  2:910567/1‑140 (MQ=255)
                                                                              gctgcCGTCAGAATAACTGTTGGCGGTATCAAAGAAATTTATGCCGCCTTCCAGTGCATGTTTAATTATGGGACGGCTGCTTTCTTCCGGCAGTGTCCATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGc                                                        <  1:939060/140‑1 (MQ=255)
                                                                                     tCAGAATAACTGTTGGCGGTATCAAAGAAATTTATGTCGCCTTCCAGTGCATGTTTAATTATGGGACGGCTGCTTTCTTCCGGCAGTGTCCATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCa                                                 <  2:1040995/140‑1 (MQ=255)
                                                                                     tCAGAATAACTGTTGGCGGTATCAAAGAAATTTATGCCGCCTTCCAGTGCATGTTTAATTATGGGACGGCTGCTTTCTTCCGGCAGTGTCCATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCa                                                 <  1:954960/140‑1 (MQ=255)
                                                                                              cTGTTGGCGGTATCAAAGAAATTTATGCCGCCTTCCAGTGCATGTTTAATTATGGGACGGCTGCTTTCTTCCGGCAGTGTCCATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGGa                                        <  1:779416/140‑1 (MQ=255)
                                                                                                   ggCGGTATCAAAGAAATTTATGCCGCCTTCCAGTGCATGTTTAATTATGGGACGGCTGCTTTCTTCCGGCAGTGTCCATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGGAAAcgc                                   >  1:388313/1‑140 (MQ=255)
                                                                                                      ggTATCAAAGAAATTTATGCCGCCTTCCAGTGCATGTTTAATTATGGGACGGCTGCTTTCTTCCGGCAGTGTCCATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGGAAACGCGaa                                >  1:1273200/1‑140 (MQ=255)
                                                                                                        tATCAAAGAAATTTATGCCGCCTTCCAGTGCATGTTTAATTATGGGACGGCTGCTTTCTTCCGGCAGTGTCCATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGGAAACGCGAAgg                              >  1:1310056/1‑140 (MQ=255)
                                                                                                              aGAAATTTATGCCGCCTTCCAGTGCATGTTTAATTATGGGACGGCTGCTTTCTTCCGGCAGTGTCCATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGGAAACGCGAAGGTCGGtt                        <  1:899653/140‑1 (MQ=255)
                                                                                                                  aTTTATGCCGCCTTCCAGTGCATGTTTAATTATGGGACGGCTGCTTTCTTCCGGCAGTGTCCATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGGAAACGCGAAGGTCGGTTTTTc                    >  1:737866/1‑140 (MQ=255)
                                                                                                                     tATGCCGCCTTCCAGTGCATGTTTAATTATGGGACGGCTGCTTTCTTCCGGCAGTGTCCATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGGAAACGCGAAGGTCGGTTTTTCCTa                 <  2:1273200/140‑1 (MQ=255)
                                                                                                                     tATGCCGCCTTCCAGTGCATGTTTAATTATGGGACGGCTGCTTTCTTCCGGCAGTGTCCATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGGAAACGCGAAGGTCGGTTTTTCCTa                 <  1:427848/140‑1 (MQ=255)
                                                                                                                       tGCCGCCTTCCAGTGCATGTTTAATTATGGGACGGCTGCTTTCTTCCGGCAGTGTCCATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGGAAACGCGAAGGTCGGTTTTTCCTAAg               <  1:1263765/140‑1 (MQ=255)
                                                                                                                          cgccTTCCAGTGCATGTTTAATTATGGGACGGCTGCTTTCTTCCGGCAGTGTCCATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGGAAACGCGAAGGTCGGTTTTTCCTAAgggg            <  1:1080599/140‑1 (MQ=255)
                                                                                                                          cgccTTCCAGTGCATGTTTAATTATGGGACGGCTGCTTTCTTCCGGCAGTGTCCATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGGAAACGCGAAGGTCGGTTTTTCCTAAgggg            <  1:1158501/140‑1 (MQ=255)
                                                                                                                          cgccTTCCAGTGCATGTTTAATTATGGGACGGCTGCTTTCTTCCGGCAGTGTCCATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGGAAACGCGAAGGTCGGTTTTTCCTAAgggg            >  1:752471/1‑140 (MQ=255)
                                                                                                                            ccTTCCAGTGCATGTTTAATTATGGGACGGCTGCTTTCTTCCGGCAGTGTCCATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGGAAACGCGAAGGTCGGTTTTTCCTAAGGGGtt          <  1:1393697/140‑1 (MQ=255)
                                                                                                                                  aGTGCATGTTTAATTATGGGACGGCTGTTTTCTTCCGGCAGTGTCCATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGGAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTg    <  2:986410/140‑1 (MQ=255)
                                                                                                                                    tGCATGTTTAATTATGGGACGGCTGCTTTCTTCCGGCAGTGTCCATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGGAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCa  >  2:676391/1‑140 (MQ=255)
                                                                                                                                        |                                                                                                                                       
ATGGAACACTTTGGTCGCAACGACCACGTCTTCACGACGGGCGAAATCCCGCAGTGCGCGACCGACGATCTCTTCGCTGCTGCCGTCAGAATAACTGTTGGCGGTATCAAAGAAATTTATGCCGCCTTCCAGTGCACGTTTAATTATGGGACGGCTGCTTTCTTCCGGCAGTGTCCATGCGTGATTACCGCGATCTGGCTCGCCAAAGGTCATACAGCCGAGGCAAAGTCGGGAAACGCGAAGGTCGGTTTTTCCTAAGGGGTTGTATTGCA  >  NC_000913/437863‑438134

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑