Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,351,991	T→C	E345E (GAA→GAG)	arcB ←	sensory histidine kinase ArcB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,351,991	0	T	C	100.0%	136.7 / NA	40	E345E (GAA→GAG)	arcB	sensory histidine kinase ArcB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (16/24);  total (16/24)

GATGCGGTAATGGCAGCGTCGGCTCCAGGTTAAAGCGCAATCCTTTTTGTTGCGCCTGCAATGCGGAGAGATTTTCCAGATCGGCAAGGAAGCTGGTGAAATCAACCGGTTGATTATCAAGCTGGACCTTGCGCCGTTCCATCTTATCCATGTCGATAATATCGTTAAAGATATTCCCCAGCGTGACGGCCGAAACATGGATGGTCTTGAGATATTTTTCCTGCTCGGCGGTGAGTTCGGTATCCAGCAGAATGCGGCTCAGACCGACGAT  >  NC_000913/3351855‑3352125                                                                                                                                         |                                                                                                                                       gATGCGGTAATGGCAGCGTCGGCTCCAGGTTAAAGCGCAATCCTTTTTGTTGCGCCTGCAATGCGGAGAGATTTTCCAGATCGGCAAGGAAGCTGGTGAAATCAACCGGTTGATTATCAAGCTGGACCTTGCGCCGCTc                                                                                                                                      >  1:232625/1‑139 (MQ=255)          aTGGCAGCGTCGGCTCCAGGTTAAAGCGCAATCCTTTTTGTTGCGCCTGCAATGCGGAGAGATTTTCCAGATCGGCAAGGAAGCTGGTGAAATCAACCGGTTGATTATCAAGCTGGACCTTGCGCCGCTCCATCTTATcc                                                                                                                            <  2:386873/140‑1 (MQ=255)           tGGCAGCGTCGGCTCCAGGTTAAAGCGCAATCCTTTTTGTTGCGCCTGCAATGCGGAGAGATTTTCCAGATCGGCAAGGAAGCTGGTGAAATCAACCGGTTGATTATCAAGCTGGACCTTGCGCCGCTCCATCTTATCCa                                                                                                                           <  2:328631/140‑1 (MQ=255)           tGGCAGCGTCGGCTCCAGGTTAAAGCGCAATCCTTTTTGTTGCGCCTGCAATGCGGAGAGATTTTCCAGATCGGCAAGGAAGCTGGTGAAATCAACCGGTTGATTATCAAGCTGGACCTTGCGCCGCTCCATCTTATCCa                                                                                                                           <  2:968425/140‑1 (MQ=255)                  gTCGGCTCCAGGTTAAAGCGCAATCCTTTTTGTTGCGCCTGCAATGCGGAGAGATTTTCCAGATCGGCAAGGAAGCTGGTGAAATCAACCGGTTGATTATCAAGCTGGACCTTGCGCCGCTCCATCTTATCCATGTCGat                                                                                                                    >  2:852077/1‑140 (MQ=255)                     ggCTCCAGGTTAAACCGCAATCCTTTTTGTTGCGCCTGCAATGCGGAGAGATTTTCCAGATCGGCAAGGAAGCTGGTGAAATCAACCGGTTGATTATCAAGCTGGACCTTGCGCCGCTCCATCTTATCCATGTCGataat                                                                                                                 <  2:1291312/140‑1 (MQ=255)                      gCTCCAGGTTAAAGCGCAATCCTTTTTGTTGCGCCTGCAATGCGGAGAGATTTTCCAGATCGGCAAGGAAGCTGGTGAAATCAACCGGTTGATTATCAAGCTGGACCTTGCGCCGCTCCATCTTATCCATGTCGataata                                                                                                                <  2:573751/140‑1 (MQ=255)                           aGGTTAAAGCGCAATCCTTTTTGTTGCGCCTGCAATGCGGAGAGATTTTCCAGATCGGCAAGGAAGCTGGTGAAATCAACCGGTTGATTATCAAGCTGGACCTTGCGCCGCTCCATCTTATCCATGTCGATAATATCGtt                                                                                                           <  1:612832/140‑1 (MQ=255)                             gTTAAAGCGCAATCCTTTTTGTTGCGCCTGCAATGCGGAGAGATTTTCCAGATCGGCAAGGAAGCTGGTGAAATCAACCGGTTGATTATCAAGCTGGACCTTGCGCCGCTCCATCTTATCCATGTCGATAATATCGTTaa                                                                                                         <  2:22261/140‑1 (MQ=255)                             gTTAAAGCGCAATCCTTTTTGTTGCGCCTGCAATGCGGAGAGATTTTCCAGATCGGCAAGGAAGCTGGTGAAATCAACCGGTTGATTATCAAGCTGGACCTTGCGCCGCTCCATCTTATCCATGTCGATAATATCGTTaa                                                                                                         <  1:483733/140‑1 (MQ=255)                                 aaGCGCAATCCTTTTTGTTGCGCCAGCAATGCGGAGAGATTTTCCAGATCGGCAAGGAAGCTGGTGAAATCAACCGGTTGATTATCAAGCTGGACCTTGCGCCGCTCCATCTTATCCATGTCGATAATATCGTTAAAGat                                                                                                     <  2:510113/140‑1 (MQ=255)                                             ttttGTTGCGCCTGCAATGCGGAGAGATTTTCCAGATCGGCAAGGAAGCTGGTGAAATCAACCGGTTGATTATCAAGCTGGACCTTGCGCCGCTCCATCTTATCCATGTCGATAATATCGTTAAAGATATTCCCCAGCGt                                                                                         <  1:1161708/140‑1 (MQ=255)                                                tgttgCGCCTGCAATGCGGAGAGATTTTCCAGATCGGCAAGGAAGCTGGTGAAATCAACCGGTTGATTATCAAGCTGGACCTTGCGCCGCTCCATCTTATCCATGTCGATAATATCGTTAAAGATATTCCCCAGCGTGAc                                                                                      <  1:852077/140‑1 (MQ=255)                                                   tgCGCCTGCAATGCGGAGAGATTTTCCAGATCGGCAAGGAAGCTGGTGAAATCAACCGGTTGATTATCTAGCTGGACCTTGCGCCGCTCCATCTTATCCATGTCGATAATATCGTTAAAGATATTCCCCAGCGTGACGGc                                                                                   <  2:981843/140‑1 (MQ=255)                                                   tgCGCCTGCAATGCGGAGAGATTTTCCAGATCGGCAAGGAAGCTGGTGAAATCAACCGGTTGATTATCAAGCTGGACCTTGCGCCGCTCCATCTTATCCATGTCGATAATATCGTTAAAGATATTCCCCAGCGTGACGGc                                                                                   <  1:603437/140‑1 (MQ=255)                                                   tgCGCCTGCAATGCGGAGAGATTTTCCAGATCGGCAAGGAAGCTGGTGAAATCAACCGGTTGATTATCAAGCTGGACCTTGCGCCGCTCCATCTTATCCATGTCGATAATATCGTTAAAGATATTCCCCAGCGTGACGGc                                                                                   >  2:772011/1‑140 (MQ=255)                                                   tgCGCCTGCAATGCGGAGAGATTTTCCAGATCGGCAAGGAAGCTGGTGAAATCAACCGGTTGATTATCAAGCTGGACCTTGCGCCGCTCCATCTTATCCATGTCGATAATATCGTTAAAGATATTCCCCAGCGTGACGGc                                                                                   >  2:33899/1‑140 (MQ=255)                                                     cgcCTGCAATGCGGAGAGATTTTCCAGATCGGCAAGGAAGCTGGTGAAATCAACCGGTTGATTATCAAGCTGGACCTTGCGCCGCTCCATCTTATCCATGTCGATAATATCGTTAAAGATATTCCCCAGCGTGACGGCCg                                                                                 >  2:713417/1‑140 (MQ=255)                                                     cgcCTGCAATGCGGAGAGATTTTCCAGATCGGCAAGGAAGCTGGTGAAATCAACCGGTTGATTATCAAGCTGGACCTTGCGCCGCTCCATCTTATCCATGTCGATAATATCGTTAAAGATATTCCCCAGCGTGACGGCCg                                                                                 >  1:1001034/1‑140 (MQ=255)                                                      gcCTGCAATGCGGAGAGATTTTCCAGATCGGCAAGGAAGCTGGTGAAATCAACCGGTTGATTATCAAGCTGGACCTTGCGCCGCTCCATCTTATCCATGTCGATAATATCGTTAAAGATATTCCCCAGCGTGACGGCCGa                                                                                <  1:33899/140‑1 (MQ=255)                                                       ccTGCAATGCGGAGAGATTTTCCAGATCGGCAAGGAAGCTGGTGAAATCAACCGGTTGATTATCAAGCTGGACCTTGCGCCGCTCCATCTTATCCATGTCGATAATATCGTTAAAGATATTCCCCAGCGTGACGGCCGaa                                                                               >  1:566693/1‑140 (MQ=255)                                                              tGCGGAGAGATTTTCCAGATCGGCAAGGAAGCTGGTGAAATCAACCGGTTGATTATCAATCTGGACCTTGCGCCGCTCCATCTTATCCATGTCGATAATATCGTTAAAGATATTCCCCAGCGTGACGGCCGAAACatgga                                                                        <  1:1054516/140‑1 (MQ=255)                                                              tGCGGAGAGATTTTCCAGATCGGCAAGGAAGCTGGTGAAATCAACCGGTTGATTATCAAGCTGGACCTTGCGCCGCTCCATCTTATCCATGTCGATAATATCGTTAAAGATATTCCCCAGCGTGACGGCCGAAACatgga                                                                        <  2:1206307/140‑1 (MQ=255)                                                              tGCGGAGAGATTTTCCAGATCGGCAAGGAAGCTGGTGAAATCAACCGGTTGATTATCAAGCTGGACCTTGCGCCGCTCCATCTTATCCATGTCGATAATATCGTTAAAGATATTCCCCAGCGTGACGGCCGAAACatgga                                                                        <  2:750387/140‑1 (MQ=255)                                                              tGCGGAGAGATTTTCCAGATCGGCAAGGAAGCTGGTGAAATCAACCGGTTGATTATCAAGCTGGACCTTGCGCCGCTCCATCTTATCCATGTCGATAATATCGTTAAAGATATTCCCCAGCGTGACGGCCGAAACatgga                                                                        <  1:273196/140‑1 (MQ=255)                                                                  gagagaTTTTCCAGATCGGCAAGGAAGCTGGTGAAATCAACCGGTTGATTATCAAGCTGGACCTTGCGCCGCTCCATCTTATCCATGTCGATAATATCGTTAAAGATATTCCCCAGCGTGACGGCCGAAACATGGATGGt                                                                    <  2:566693/140‑1 (MQ=255)                                                                   agagaTTTTCCAGATCGGCAAGGAAGCTGGTGAAATCAACCGGTTGATTATCAAGCTGGACCTTGCGCCGCTCCATCTTATCCATGTCGATAATATCGTTAAAGATATTCCCCAGCGTGACGGCCGAAACATGGATGGTc                                                                   >  2:515660/1‑140 (MQ=255)                                                                      gaTTTTCCAGATCGGCAAGGAAGCTGGTGAAATCAACCGGTTGATTATCAAGCTGGACCTTGCGCCGCTCCATCTTATCCATGTCGATAATATCGTTAAAGATATTCCCCAGCGTGACGGCCGAAACATGGATGGTCTTg                                                                >  2:124181/1‑140 (MQ=255)                                                                               gATCGGCAAGGAAGCTGGTGAAATCAACCGGTTGATTATCAAGCTGGACCTTGCGCCGCTCCATCTTATCCATGTCGATAATATCGTTAAAGATATTCCCCAGCGTGACGGCCGAAACCTGGATGGTCTTGAGATAtttt                                                       <  2:1001034/140‑1 (MQ=255)                                                                               gATCGGCAAGGAAGCTGGTGAAATCAACCGGTTGATTATCAAGCTGGACCTTGCGCCGCTCCATCTTATCCATGTCGATAATATCGTTAAAGATATTCCCCAGCGTGACGGCCGAAACATGGATGGTCTTGAGATAtttt                                                       >  2:171908/1‑140 (MQ=255)                                                                                 tCGGCAAGGAAGCTGGTGAAATCAACCGGTTGATTATCAAGCTGGACCTTGCGCCGCTCCATCTTATCCATGTCGATAATATCGTTAAAGATATTCCCCAGCGTGACGGCCGAAACATGGATGGTCTTGAGATATTTTTc                                                     >  1:7221/1‑140 (MQ=255)                                                                                      aaGGAAGCTGGTGAAATCAACCGGTTGATTATCAAGCTGGACCTTGCGCCGCTCCATCTTATCCATGTCGATAATATCGTTAAAGATATTCCCCAGCGTGACGGCCGAAACATGGATGGTCTTGAGATATTTTTCCTGCt                                                <  2:232152/140‑1 (MQ=255)                                                                                          aaGCTGGTGAAATCAACCGGTTGATTATCAAGCTGGACCTTGCGCCGCTCCATCTTATCCATGTCGATAATATCGTTAAAGATATTCCCCAGCGTGACGGCCGAAACATGGATGGTCTTGAGATATTTTTCCTGCTcggc                                            >  2:1235824/1‑140 (MQ=255)                                                                                             cTGGTGAAATCAACCGGTTGATTATCAAGCTGGACCTTGCGCCGCTCCATCTTATCCATGTCGATAATATCGTTAAAGATATTCCCCAGCGTGACGGCCGAAACATGGATGGTCTTGAGATATTTTTCCTGCTCGGCGGt                                         <  1:109478/140‑1 (MQ=255)                                                                                                              ttGATTATCAAGCTGGACCTTGCGCCGCTCCATCTTATCCATGTCGATAATATCGTTAAAGATATTCCCCAGCGTGACGGCCGAAACATGGATGGTCTTGAGATATTTTTCCTGCTCGGCGGTGAGTTCGGTATCcagca                        <  1:257444/140‑1 (MQ=255)                                                                                                               tGATTATCAAGCTGGACCTTGCGCCGCTCCATCTTATCCATGTCGATAATATCGTTAAAGATATTCCCCAGCGTGACGGCCGAAACATGGATGGTCTTGAGATATTTTTCCTGCTCGGCGGTGAGTTCGGTATCcagcag                       >  2:22108/1‑140 (MQ=255)                                                                                                                   tATCAAGCTGGACCTTGCGCCGCTCCATCTTATCCATGTCGATAATATCGTTAAAGATATTCCCCAGCGTGACGGCCGAAACATGGATGGTCTTGAGATATTTTTCCTGCTCGGCGGTGAGTTCGGTATCCAGCAGAATg                   >  1:260706/1‑140 (MQ=255)                                                                                                                        aGCTGGACCTTGCGCCGCTCCATCTTATCCATGTCGATAATATCGTTAAAGATATTCCCCAGCGTGACGGCCGAAACATGGATGGTCTTGAGATATTTTTCCTGCTCGGCGGTGAGTTCGGTATCCAGCAGAATGCGGCt              >  1:445702/1‑140 (MQ=255)                                                                                                                             gACCTTGCGCCGCTCCATCTTATCCATGTCGATAATATCGTTAAAGATATTCCCCAGCGTGACGGCCGAAACATGGATGGTCTTGAGATATTTTTCCTGCTCGGCGGTGAGTTCGGTATCCAGCAGAATGCGGCTCAGAc         <  1:124181/140‑1 (MQ=255)                                                                                                                                   gcgcCGCTCCATCTTATCCATGTCGATAATATCGTTAAAGATATTCCCCAGCGTGACGGCCGAAACATGGATGGTCTTGAGATATTTTTCCTGCTCGGCGGTGAGTTCGGTATCCAGCAGAATGCGGCTCAGACcgacga   >  1:147874/1‑140 (MQ=255)                                                                                                                                    cgccgcTCCATCTTATCCATGTCGATAATATCGTTAAAGATATTCCCCAGCGTGACGGCCGAAACATGGATGGTCTTGAGATATTTTTCCTGCTCGGCGGTGAGTTCGGTATCCAGCAGAATGCGGCTCAGACCGACGAt  >  2:1185958/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                       GATGCGGTAATGGCAGCGTCGGCTCCAGGTTAAAGCGCAATCCTTTTTGTTGCGCCTGCAATGCGGAGAGATTTTCCAGATCGGCAAGGAAGCTGGTGAAATCAACCGGTTGATTATCAAGCTGGACCTTGCGCCGTTCCATCTTATCCATGTCGATAATATCGTTAAAGATATTCCCCAGCGTGACGGCCGAAACATGGATGGTCTTGAGATATTTTTCCTGCTCGGCGGTGAGTTCGGTATCCAGCAGAATGCGGCTCAGACCGACGAT  >  NC_000913/3351855‑3352125

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑