Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,544,214	G→A	P211S (CCG→TCG)	bioH ←	pimeloyl‑acyl carrier protein methyl ester esterase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,544,214	0	G	A	100.0%	206.1 / NA	60	P211S (CCG→TCG)	bioH	pimeloyl‑acyl carrier protein methyl ester esterase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (30/30);  total (30/30)

TCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTGGCGAAGATATATGATTCGCTGTGAGGCCAAAGTTTATCCAGCATCGGCACCACTTTGCGCGGCACCAGACCGTCGAGATAGCCATACAATCGCAAAAACGGCATGGACACGTTTTGCAGCGGCTGACGGAGATCGACCGTTTTCAGGATTTCCAGCCCGCCATTAAGCACGTCAACCTCCGGCATCGGTAACGCCAG  >  NC_000913/3544082‑3544349                                                                                                                                     |                                                                                                                                        tctGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTGGCGAAGATATATGATTCGCTGTGAGGCCAAAGTTTATCCAGCATCGGCACCACTTTGCGCGACACCa                                                                                                                                    >  1:725021/1‑138 (MQ=255) tctGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTGGCGAAGATATATGATTCGCTGTGAGGCCAAAGTTTATCCAGCATCGGCACCACTTTGCGCGACACCa                                                                                                                                    <  2:725021/138‑1 (MQ=255)   tGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTGGCGAAGATATATGATTCGCTGTGAGGCCAAAGTTTATCCAGCATCGGCACCACTTTGCGCGACACCAGAcc                                                                                                                                >  1:1059856/1‑140 (MQ=255)     cTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTGGCGAAGATATATGATTCGCTGTGAGGCCAAAGTTTATCCAGCATCGGCACCACTTTGCGCGACACCAGACCGt                                                                                                                              >  1:785968/1‑140 (MQ=255)     cTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTGGCGAAGATATATGATTCGCTGTGAGGCCAAAGTTTATCCAGCATCGGCACCACTTTGCGCGACACCAGACCGt                                                                                                                              <  1:1295216/140‑1 (MQ=255)        cAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTGGCGAAGATATATGATTCGCTGTGAGGCCAAAGTTTATCCAGCATCGGCACCACTTTGCGCGACACCAGACCGTCga                                                                                                                           <  1:537047/140‑1 (MQ=255)        cAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTGGCGAAGATATATGATTCGCTGTGAGGCCAAAGTTTATCCAGCATCGGCACCACTTTGCGCGACACCAGACCGTCga                                                                                                                           <  2:414095/140‑1 (MQ=255)        cAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTGGCGAAGATATATGATTCGCTGTGAGGCCAAAGTTTATCCAGCATCGGCACCACTTTGCGCGACACCAGACCGTCga                                                                                                                           <  2:642450/140‑1 (MQ=255)        cAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTGGCGAAGATATATGATTCGCTGTGAGGCCAAAGTTTATCCAGCATCGGCACCACTTTGCGCGACACCAGACCGTCga                                                                                                                           <  2:89340/140‑1 (MQ=255)        cAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTGGCGAAGATATATGATTCGCTGTGAGGCCAAAGTTTATCCAGCATCGGCACCACTTTGCGCGACACCAGACCGTCga                                                                                                                           <  2:899077/140‑1 (MQ=255)        cAACCCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTGGCGAAGATATATGATTCGCTGTGAGGCCAAAGTTTATCCAGCATCGGCACCACTTTGCGCGACACCAGACCGTCga                                                                                                                           <  2:1299003/140‑1 (MQ=255)          aCGCCCCCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTGGCGAAGATATATGATTCGCTGTGAGGCCAAAGTTTATCCAGCATCGGCACCACTTTGCGCGACTCCAGACCGTCgaga                                                                                                                         <  2:162314/140‑1 (MQ=255)             ccaccaGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTGGCGAAGATATATGATTCGCTGTGAGGCCAAAGTTTATCCAGCATCGGCACCACTTTGCGCGACACCAGACCGTCGAGATAg                                                                                                                      >  1:1112869/1‑140 (MQ=255)                   gcagGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTGGCGAAGATATATGATTCGCTGTGAGGCCAAAGTTTATCCAGCATCGGCACCACTTTGCGCGACACCAGACCGTCGAGATAGCCATa                                                                                                                 >  1:316968/1‑139 (MQ=255)                   gcagGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTGGCGAAGATATATGATTCGCTGTGAGGCCAAAGTTTATCCAGCATCGGCACCACTTTGCGCGACACCAGACCGTCGAGATAGCCATa                                                                                                                 <  2:316968/139‑1 (MQ=255)                    cagGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTGGCGAAGATATATGATTCGCTGTGAGGCCAAAGTTTATCCAGCATCGGCACCACTTTGCGCGACACCAGACCGTCGAGATAGCCATACa                                                                                                               >  1:1048620/1‑140 (MQ=255)                          aCAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTGGCGAAGATATATGATTCGCTGTGAGGCCAAAGTTTATCCAGCATCGGCACCACTTTGCGCGACACCAGACCGTCGAGATAGCCATACAATCGCa                                                                                                         <  1:1142104/140‑1 (MQ=255)                                 tCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTGGCGAAGATATATGATTCGCTGTGAGGCCAAAGTTTATCCAGCATCGGCACCACTTTGCGCGACACCAGACCGTCGAGATAGCCATACAATCGCAAAAACgg                                                                                                  >  1:1142518/1‑140 (MQ=255)                                 tCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTGGCGAAGATATATGATTCGCTGTGAGGCCAAAGTTTATCCAGCATCGGCACCACTTTGCGCGACACCAGACCGTCGAGATAGCCATACAATCGCAAAAACgg                                                                                                  <  2:594672/140‑1 (MQ=255)                                 tCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTGGCGAAGATATATGATTCGCTGTGAGGCCAAAGTTTATCCAGCATCGGCACCACTTTGCGCGACACCAGACCGTCGAGATAGCCATACAATCGCAAAAACgg                                                                                                  <  2:921833/140‑1 (MQ=255)                                   ggCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTGGCGAAGATATATGATTCGCTGTGAGGCCAAAGTTTATCCAGCATCGGCACCACTTTGCGCGACACCAGACCGTCGAGATAGCCATACAATCGCAAAAACGGCa                                                                                                >  1:512466/1‑140 (MQ=255)                                        gATGCGAAATAAATGGCGCATGGGCCGCTTTGGCGAAGATATATGATTCGCTGTGAGGCCAAAGTTTATCCAGCATCGGCACCACTTTGCGCGACACCAGACCGTCGAGATAGCCATACAATCGCAAAAACGGCATGGac                                                                                           <  2:1142518/140‑1 (MQ=255)                                                  aaatGGCGCATGGGCCGCTTTGGCGAAGATATATGATTCGCTGTGAGGCCAAAGTTTATCCAGCATCGGCACCACCTTGCGCGACACCAGACCGTCGAGATAGCCATACAATCGCAAAAACGGCATGGACACGTTTTGCa                                                                                 <  2:1313332/140‑1 (MQ=255)                                                        cgcATGGGCCGCTTTGGCGAAGATATATGATTCGCTGTGAGGCCAAAGTTTATCCAGCATCGGCACCACTTTGCGCGACACCAGACCGTCGAGATAGCCATACAATCGCAAAAACGCCAGGGCACCGTTTTCCAGCGGcg                                                                           >  2:455327/1‑139 (MQ=255)                                                            tGGGCCGCTTTGGCGAAGATATATGATTCGCTGTGAGGCCAAAGTTTATCCAGCATCGGCACCACTTTGCGCGACACCAGACCGTCGAGATAGCCATACAATCGCAAAAACGGCATGGACACGTTTTGCAGCGGCTGACg                                                                       <  2:785968/140‑1 (MQ=255)                                                              ggCCGCTTTGGCGAAGATATATGATTCGCTGTGAGGCCAAAGTTTATCCAGCATCGGCACCACTTTGCGCGACACCAGACCGTCGAGATAGCCATACAATCGCAAAAACGGCATGGACACGTTTTGCAGCGGCTGACGga                                                                     <  2:536837/140‑1 (MQ=255)                                                                  gCTTTGGCGAAGATATATGATTCGCTGTGAGGCCAAAGTTTATCCAGCATCGGCACCACTTTGCGCGACACCAGACCGTCGAGATAGCCATACAATCGCAAAAACGGCATGGACACGTTTTGCAGCGGCTGACGGAGATc                                                                 >  2:1386115/1‑140 (MQ=255)                                                                   cTTTGGCGAAGATATATGATTCGCTGTGAGGCCAAAGTTTATCCAGCATCGGCACCACTTTGCGCGACACCAGACCGTCGAGATAGCCATACAATCGCAAAAACGGCATGGACACGTTTTGCAGCGGCTGACGGAGATCg                                                                >  2:274602/1‑140 (MQ=255)                                                                   cTTTGGAGAAGATATATGATTCGCTGTGAGGCCAAAGTTTATCCAGCATCGGCACCACTTTGCGCGACACCAGACCGTCGAGATAGCCATACAATCGCAAAAACGGCATGGACACGTTTTGCAGCGGCTGACGGAGATCg                                                                >  2:222311/1‑140 (MQ=255)                                                                    tttGGCGAAGATATATGATTCGCTGTGAGGCCAAAGTTTATCCAGCATCGGCACCACTTTGCGCGACACCAGACCGTCGAGATAGCCATACAATCGCAAAAACGGCATGGACACGTTTTGCAGCGGCTGACGGAGATCGa                                                               <  1:164421/140‑1 (MQ=255)                                                                     ttGGCGAAGATATATGATTCGCTGTGAGGCCAAAGTTTATCCAGCATCGGCACCACTTTGCGCGACACCAGACCGTCGAGATAGCCATACAATCGCAAAAACGGCATGGACACGTTTTGCAGCGGCTGACGGAGATCGAc                                                              <  2:512466/140‑1 (MQ=255)                                                                      tGGCGAAGATATATGATTCGCTGTGAGGCCAAAGTTTATCCAGCATCGGCACCACTTTGCGCGACACCAGACCGTCGAGATAGCCATACAATCGCAAAAACGGCATGGACACGTTTTGCAGCGGCTGACGGAGATCGAcc                                                             <  2:1204732/140‑1 (MQ=255)                                                                          gAAGATATATGATTCGCTGTGAGGCCAAAGTTTATCCAGCATCGGCACCACTTTGCGCGACACCAGACCGTCGAGATAGCCATACAATCGCAAAAACGGCATGGACACGTTTTGCAGCGGCTGACGGAGATCGACCGttt                                                         <  1:979190/140‑1 (MQ=255)                                                                                       tCGCTGTGAGGCCAAAGTTTATCCAGCATCGGCACCACTTTGCGCGACACCAGACCGTCGAGATAGCCATACAATCGCAAAAACGGCATGGACACGTTTTGCAGCGGCTGACGGAGATCGACCGTTTTCAGGATTTCCAg                                            >  1:1074744/1‑140 (MQ=255)                                                                                       tCGCTGTGAGGCCAAAGTTTATCCAGCATCGGCACCACTTTGCGCGACACCAGACCGTCGAGATAGCCATACAATCGCAAAAACGGCATGGACACGTTTTGCAGCGGCTGACGGAGATCGACCGTTTTCAGGATTTCCAg                                            >  1:488883/1‑140 (MQ=255)                                                                                        cGCTGTGAGGCCAAAGTTTATCCAGCATCGGCACCACTTTGCGCGACACCAGACCGTCGAGATAGCCATACAATCGCAAAAACGGCATGGACACGTTTTGCAGCGGCTGACGGAGATCGACCGTTTTCAGGATTTCCAGc                                           >  2:986664/1‑140 (MQ=255)                                                                                        cGCTGTGAGGCCAAAGTTTATCCAGCATCGGCACCACTTTGCGCGACACCAGACCGTCGAGATAGCCATACAATCGCAAAAACGGCATGGACACGTTTTGCAGCGGCTGACGGAGATCGACCGTTTTCAGGATTTCCAGc                                           >  2:15794/1‑140 (MQ=255)                                                                                        cGCTGTGAGGCCAAAGTTTATCCAGCATCGGCACCACTTTGCGCGACACCAGACCGTCGAGATAGCCATACAATCGCAAAAACGGCATGGACACGTTTTGCAGCGGCTGACGGAGATCGACCGTTTTCAGGATTTCCAGc                                           >  1:516100/1‑140 (MQ=255)                                                                                         gCTGTGAGGCCAAATTTTATCCAGCATCGGCACCACTTTGCGCGACACCAGACCGTCGAGATAGCCATACAATCGCAAAAACGGCATGGACACGTTTTGCAGCGGCTGACGGAGATCGACCGTTTTCAGGATTTCCAGcc                                          <  1:939255/140‑1 (MQ=255)                                                                                         gCTGTGAGGCCAAAGTTTATCCAGCATCGGCACCACTTTGCGCGACACCAGACCGTCGAGATAGCCATACAATCGCAAAAACGGCATGGACACGTTTTGCAGCGGCTGACGGAGATCGACCGTTTTCAGGATTTCCAGcc                                          <  1:986664/140‑1 (MQ=255)                                                                                         gCTGTGAGGCCAAAGTTTATCCAGCATCGGCACCACTTTGCGCGACACCAGACCGTCGAGATAGCCATACAATCGCAAAAACGGCATGGACACGTTTTGCAGCGGCTGACGGAGATCGACCGTTTTCAGGATTTCCAGcc                                          >  2:952487/1‑140 (MQ=255)                                                                                         gCTGTGAGGCCAAAGTTTATCCAGCATCGGCACCACTTTGCGCGACACCAGACCGTCGAGATAGCCATACAATCGCAAAAACGGCATGGACACGTTTTGCAGCGGCTGACGGAGATCGACCGTTTTCAGGATTTCCAGcc                                          >  1:11429/1‑140 (MQ=255)                                                                                              gAGGCCAAAGTTTATCCAGCATCGGCACCACTTTGCGCGACACCAGACCGTCGAGATAGCCATACAATCGCAAAAACGGCATGGACACGTTTTGCAGCGGCTGACGGAGATCGACCGTTTTCAGGATTTCCAGCCCGCCa                                     <  2:1112869/140‑1 (MQ=255)                                                                                                   cAAAGTTTATCCAGCATCGGCACCACTTTGCGCGACACCAGACCGTCGAGATAGCCATACAATCGCAAAAACGGCATGGACACTTTTGGCGGCGGCTGACGGAGATCGACCGTTTTCAGGATTTCCAGCCCGCCATTAAg                                <  2:275269/140‑1 (MQ=255)                                                                                                     aaGTTTATCCAGCATCGGCACCACTTTGCGCGACACCAGACCGTCGAGATAGCCATACAATCGCAAAAACGGCATGGACACGTTTTGCAGCGGCTGACGGAGATCGACCGTTTTCAGGATTTCCAGCCCGCCATTAAGCa                              >  1:401216/1‑140 (MQ=255)                                                                                                       gTTTATCCAGAATCGGCACCACTTTGCGCGAGACCAGACCGTCGAGATAGCCATACAATCGCAAAAACGGCATGGAGAGGTTTTGCAGCGGCTGACGGAGATCGACCGTTTTCAGGATTTCCAGTCCGCCCTTAAGCACg                            >  2:1126876/1‑140 (MQ=255)                                                                                                         ttATCCAGCATCGGCACCACTTTGCGCGACACCAGACCGTCGAGATAGCCATACAATCGCAAAAACGGCATGGACACGTTTTGCAGCGGCTGACGGAGATCGACCGTTTTCAGGATTTCCAGCCCGCCATTAAGCACGTc                          <  1:364131/140‑1 (MQ=255)                                                                                                            tCCAGCATCGGCACCACTTTGCGCGACACCAGACCGTCGAGATAGCCATACAATCGCAAAAACGGCATGGACACGTTTTGCAGCGGCTGACGGAGATCGACCGTTTTCAGGGTTTCCAGCCCGCCATTAAGCACGGCAAc                       >  1:543249/1‑140 (MQ=255)                                                                                                            tCCAGCATCGGCACCACTTTGCGCGACACCAGACCGTCGAGATAGCCATACAATCGCAAAAACGGCATGGACACGTTTTGCAGCGGCTGACGGAGATCGACCGTTTTCAGGATTTCCAGCCCGCCATTAAGCACGTCAAc                       >  1:369081/1‑140 (MQ=255)                                                                                                              cAGCATCGGCACCACTTTGCGCGACACCAGACCGTCGAGATAGCCATACAATCGCAAAAACGGCATGGACACGTTTTGCAGCGGCTGACGGAGATCGACCGTTTTCAGGATTTCCAGCCCGCCATTAAGCACGTCAACCt                     <  1:487431/140‑1 (MQ=255)                                                                                                                gCATCGGCACCACTTTGCGCGACACCAGACCGTCGAGATAGCCATACAATCGCAAAAACGGCATGGACACGTTTTGCAGCGGCTGACGGAGATCGACCGTTTTCAGGATTTCCAGCCCGCCATTAAGCACGTCAACCTcc                   >  2:1330381/1‑140 (MQ=255)                                                                                                                 cATCGGCACCACTTTGCGCGACACCAGACCGTCGAGATAGCCATACAATCGCAAAAACGGCATGGACACGTTTTGCAGCGGCTGACGGAGATCGACCGTTTTCAGGATTTCCAGCCCGCCATTAAGCACGTCAACCTCCg                  >  1:1097760/1‑140 (MQ=255)                                                                                                                  aTCGGCACCACTTTGCGCGACACCAGACCGTCGAGATAGCCATACAATCGCAAAAACGGCATGGACACGTTTTGCAGCGGCTGACGGAGATCGACCGTTTTCAGGATTTCCAGCCCGCCATTAAGCACGTCAACCTCCgg                 >  1:1367027/1‑140 (MQ=255)                                                                                                                    cGGCACCACTTTGCGCGACACCAGACCGTCGAGATAGCCATACAATCGCAAAAACGGCATGGACACGTTTTGCAGCGGCTGACGGAGATCGACCGTTTTCAGGATTTCCAGCCCGCCATTAAGCACGTCAACCTCCGGCa               <  2:488883/140‑1 (MQ=255)                                                                                                                    cGGCACCACTTTGCGCGACACCAGACCGTCGAGATAGCCATACAATCGCAAAAACGGCATGGACACGTTTTGCAGCGGCTGACGGAGATCGACCGTTTTCAGGATTTCCAGCCCGCCATTAAGCACGTCAACCTCCGGCa               <  1:15794/140‑1 (MQ=255)                                                                                                                    cGGCACCACTTTGCGCGACACCAGACCGTCGAGATAGCCATACAATCGCAAAAACGGCATGGACACGTTTTGCAGCGGCTGACGGAGATCGACCGTTTTCAGGATTTCCAGCCCGCCATTAAGCACGTCAACCTCCGGCa               <  1:125207/140‑1 (MQ=255)                                                                                                                      gCACCACTTTGCGCGACACCAGACCGTCGAGATAGCCATACAATCGCAAAAACGGCATGGACACGTTTTGCAGCGGCTGACGGAGATCGACCGTTTTCAGGATTTCCAGCCCGCCATTAAGCACGTCAACCTCCGGCATc             >  1:535833/1‑140 (MQ=255)                                                                                                                      gCACCACTTTGCGCGACACCAGACCGTC‑‑GATAGCCATACAATCGCAAAAACGGCATGGACACGTTTTGCAGCGGCTGACGGAGATCGACCGTTTTCAGGATTTCCAGCCCGCCATTAAGCACGTCAACCTCCGGCATCgg           >  1:811884/1‑140 (MQ=255)                                                                                                                          cacTTTGCGCGACACCAGACCGTCGAGATAGCCATACAATCGCAAAAACGGCATGGACACGTTTTGCAGCGGCTGACGGAGATCGACCGTTTTCAGGATTTCCAGCCCGCCATTAAGCACGTCAACCTCCGGCATCGGTa         <  1:1386115/140‑1 (MQ=255)                                                                                                                            cTTTGCGCGACACCAGCCCGTCGAGATAGCCATACAATCGCAAAAACGGCATGGACACGTTTTGCAGCGGCTGACGGAGATCGACCGTTTTCAGGATTTCCAGCCCGCCATTAAGCACGTCAACCTCCGGc                <  2:801303/131‑1 (MQ=255)                                                                                                                            cTTTGCGCGACACCAGACCGTCGAGATAGCCATACAATCGCAAAAACGGCATGGACACGTTTTGCAGCGGCTGACGGAGATCGACCGTTTTCAGGATTTCCAGCCCGCCATTAAGCACGTCAACCTCCGGc                >  1:801303/1‑131 (MQ=255)                                                                                                                            cTTTGCGCGACACCAGACCGTCGAGATAGCCATACAATCGCAAAAACGGCATGGACACGTTTTGCAGCGGCTGACGGAGATCGACCGTTTTCAGGATTTCCAGCCCGCCATTAAGCACGTCAACCTCCGGCATCGGTAAc       >  2:717141/1‑140 (MQ=255)                                                                                                                                 cgcgACACCAGACCGTCGAGATAGCCATACAATCGCAAAAACGGCATGGACACGTTTTGCAGCGGCTGACGGAGATCGACCGTTTTCAGGATTTCCAGCCCGCCATTAAGCACGTCAACCTCCGGCATCGGTAACGCCAg  >  1:556301/1‑140 (MQ=255)                                                                                                                                     |                                                                                                                                        TCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTGGCGAAGATATATGATTCGCTGTGAGGCCAAAGTTTATCCAGCATCGGCACCACTTTGCGCGGCACCAGACCGTCGAGATAGCCATACAATCGCAAAAACGGCATGGACACGTTTTGCAGCGGCTGACGGAGATCGACCGTTTTCAGGATTTCCAGCCCGCCATTAAGCACGTCAACCTCCGGCATCGGTAACGCCAG  >  NC_000913/3544082‑3544349

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑