Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,587,299	G→A	M119I (ATG→ATA)	yhhA →	DUF2756 domain‑containing protein YhhA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,587,299	0	G	A	100.0%	198.3 / NA	58	M119I (ATG→ATA)	yhhA	DUF2756 domain‑containing protein YhhA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (29/29);  total (29/29)

ATAATTCTCAGCGGGTGTTGCAGTCACAGCCGGGGGAGCGAAATCCCGCCCGGCAGCAAATGCTGCCCAACACCAACGGCGGGATGTTAAACAGCAACCGTAATCCGGATAGTTCGTTGAATCAGCAGCATATGCTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAACTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCA  >  NC_000913/3587166‑3587435                                                                                                                                      |                                                                                                                                         ataatTCTCAGCGGGTGTTGCAGTCACAGCCGGGGGAGCGAAATCCCGCCCGGCAGCAAATGCTGCCCAACACCAACGGCGGGATGTTAAACAGCAACCGTAATCCGGATAGTTCGTTGAATCAGCAGCATATACTGCCg                                                                                                                                    <  1:60087/140‑1 (MQ=255)  taatTCTCAGCGGGTGTTGCAGTCACAGCCGGGGGAGCGAAATCCCGCCCGGCAGCAAATGCTGCCCAACACCAACGGCGGGATGTTAAACAGCAACCGTAATCCGGATAGTTCGTTGAATCAGCAGCATATACTGCCgg                                                                                                                                   <  1:999930/140‑1 (MQ=255)      tctcAGCGGGTGTTGCAGTCACAGCCGGGGGAGCGAAATCCCGCCCGGCAGCAAATGCTGCCCAACACCAACGGCGGGATGTTAAACAGCAACCGTAATCCGGATAGTTCGTTGAATCAGCAGCATATACTGCCggagag                                                                                                                               <  1:1119825/140‑1 (MQ=255)        tcAGCGGGTGTTGCAGTCACAGCCGGGGGAGCGAAATCCCGCCCGGCAGCAAATGCTGCCCAACACCAACGGCGGGATGTTAAACAGCAACCGTAATCCGGATAGTTCGTTGAATCAGCAGCATATACTGCCggagagga                                                                                                                             <  2:176095/140‑1 (MQ=255)        tcAGCGGGTGTTGCAGTCACAGCCGGGGGAGCGAAATCCCGCCCGGCAGCAAATGCTGCCCAACACCAACGGCGGGATGTTAAACAGCAACCGTAATCCGGATAGTTCGTTGAATCAGCAGCATATACTGCCggagagga                                                                                                                             <  2:143238/140‑1 (MQ=255)            cGGGTGTTGCAGTCACAGCCGGGGGAGCGAAATCCCGCCCGGCAGCAAATGCTGCCCAACACCAACGGCGGGATGTTAAACAGCAACCGTAATCCGGATAGTTCGTTGAATCAGCAGCATATACTGCCGGAGAGGAGaaa                                                                                                                         >  1:339103/1‑140 (MQ=255)            cGGGTGTTGCAGTCACAGCCGGGGGAGCGAAATCCCGCCCGGCAGCAAATGCTGCCCAACACCAACGGCGGGATGTTAAACAGCAACCGTAATCCGGATAGTTCGTTGAATCAGCAGCATATACTGCCGGAGAGGAGaaa                                                                                                                         >  2:416115/1‑140 (MQ=255)                tgtTGCAGTCACAGCCGGGGGAGCGAAATCCCGCCCGGCAGCAAATGCTGCCCAACACCAACGGCGGGATGTTAAACAGCAACCGTAATCCGGATAGTTCGTTGAATCAGCAGCATATACTGCCGGAGAGGAGAAACGGc                                                                                                                     >  1:28560/1‑140 (MQ=255)                    gCAGTCACAGCCGGGGGAGCGAAATCCCGCCCGGCAGCAAATGCTGCCCAACACCAACGGCGGGATGTTAAACAGCAACCGTAATCCGGATAGTTCGTTGAATCAGCAGCATATACTGCCGGAGAGGAGaaa                                                                                                                         >  2:1114344/1‑132 (MQ=255)                    gCAGTCACAGCCGGGGGAGCGAAATCCCGCCCGGCAGCAAATGCTGCCCAACACCAACGGCGGGATGTTAAACAGCAACCGTAATCCGGATAGTTCGTTGAATCAGCAGCATATACTGCCGGAGAGGAGaaa                                                                                                                         <  1:1114344/132‑1 (MQ=255)                       gTCACAGCCCGGGGAGCGAAATCCCGCCCGGCAGCAAATGCTGCCCAACACCAACGGCGGGATGTTAAACAGCAACCGTAATCCGGATAGTTCGTTGAATCAGCAGCATATACTGCCGGAGAGGAGAAACGGCGACATGc                                                                                                              <  1:546525/140‑1 (MQ=255)                        tCACAGCCGGGGGAGCGAAATCCCGCCCGGCAGCAAATGCTGCCCAACACCAACGGCGGGATGTTAAACAGCAACCGTAATCCGGATAGTTCGTTGAATCAGCAGCATATACTGCCGGAGAGGAGAAACGGCGACATGCt                                                                                                             >  1:149719/1‑140 (MQ=255)                          acaGCCGGGGGAGCGAAATCCCGCCCGGCAGCAAATGCTGCCCAACACCAACGGCGGGATGTTAAACAGCAACCGTAATCCGGGTAGTTCGTTGAATCAGCAGCATATACTGCCGGAGAGGAGAAACGGCGACATGCTGa                                                                                                           >  2:581746/1‑140 (MQ=255)                          acaGCCGGGGGAGCGAAATCCCGCCCGGCAGCAAATGCTGCCCAACACCAACGGCGGGATGTTAAACAGCAACCGTAATCCGGATAGTTCGTTGAATCAGCAGCATATACTGCCGGAGAGGAGAAACGGCGACATGCTGa                                                                                                           >  1:935341/1‑140 (MQ=255)                          acaGCCGGGGGAGCGAAATCCCGCCCGGCAGCAAATGCTGCCCAACACCAACGGCGGGATGTTAAACAGCAACCGTAATCCGGATAGTTCGTTGAATCAGCAGCATATACTGCCGGAGAGGAGAAACGGCGACATGCTGa                                                                                                           >  1:846017/1‑140 (MQ=255)                            aGCCGGGGGAGCGAAATCCCGCCCGGCAGCAAATGCTGCCCAACACCAACGGCGGGATGTTAAACAGCAACCGTAATCCGGATAGTTCGTTGAATCAGCAGCATATACTGCCGGAGAGGAGAAACGGCGACATGCTGAAt                                                                                                         <  2:120668/140‑1 (MQ=255)                            aGCCGGGGGAGCGAAATCCCGCCCGGCAGCAAATGCTGCCCAACACCAACGGCGGGATGTTAAACAGCAACCGTAATCCGGATAGTTCGTTGAATCAGCAGCATATACTGCCGGAGAGGAGAAACGGCGACATGCTGAAt                                                                                                         <  1:1272545/140‑1 (MQ=255)                             gCCGGGGGAGCGAAATCCCGCCCGGCAGCAAATGCTGCCCAACACCAACGGCGGGATGTTAAACAGCAACCGTAATCCGGATAGTTCGTTGAATCAGCAGCATATACTGCCGGAGAGGAGAAACGGCGACATGCTGAATc                                                                                                        >  2:605573/1‑140 (MQ=255)                              ccGGGGGAGCGAAATCCCGCCCGGCAGCAAATGCTGCCCAACACCAACGGCGGGATGTTAAACAGCAACCGTAATCCGGATAGTTCGTTGAATCAGCAGCATATACTGCCGGAGAGGAGAAACGGCGACATGCTGAATCa                                                                                                       >  2:1399516/1‑140 (MQ=255)                                 ggggAGCGAAATCCCGCCCGGCAGCAAATGCTGCCCAACACCAACGGCGGGATGTTAAACAGCAACCGTAATCCGGATAGTTCGTTGAATCAGCAGCATATACTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGcc                                                                                                    >  2:197901/1‑140 (MQ=255)                                     aGCGAAATCCCGCCCGGCAGCAAATGCTGCCCAACACCAACGGCGGGATGTTAAACAGCAACCGTAATCCGGATAGTTCGTTGAATCAGCAGCATATACTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGc                                                                                                <  2:1250885/140‑1 (MQ=255)                                         aaaTCCCGCCCGGCAGCAAATGCTGCCCAACACCAACGGCGGGATGTTAAACAGCAACCGTAATCCGGATAGTTCGTTGAATCAGCAGCATATACTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCAcgc                                                                                            <  1:605573/140‑1 (MQ=255)                                           aTCCCGCCCGGCAGCAAATGCTGCCCAACACCAACGGCGGGATGTTAAACAGCAACCGTAATCCGGATAGTTCGTTGAATCAGCAGCATATACTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCAcgccg                                                                                          <  2:542119/140‑1 (MQ=255)                                            tCCCGCCCGGCAGCAAATGCTGCCCAACACCAACGGCGGGATGTTAAACAGCAACCGTAATCCGGATAGTTCGTTGAATCAGCAGCATATACTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCAcgccgc                                                                                         >  1:8164/1‑140 (MQ=255)                                            tCCCGCCCGGCAGCAAATGCTGCCCAACACCAACGGCGGGATGTTAAACAGCAACCGTAATCCGGATAGTTCGTTGAATCAGCAGCATATACTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCAcgccgc                                                                                         >  1:215892/1‑140 (MQ=255)                                                 cccgGCAGCAAATGCTGCCCAACACCAACGGCGGGATGTTAAACAGCAACCGTAATCCGGATAGTTCGTTGAATCAGCAGCATATACTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCt                                                                                    >  2:56748/1‑140 (MQ=255)                                                     gcagcaAATGCTGCCCAACACCAACGGCGGGATGTTAAACAGCAACCGTAATCCGGATAGTTCGTTGAATCAGCAGCATATACTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACa                                                                                <  1:56748/140‑1 (MQ=255)                                                               ctgcCCAACACCAACGGCGGGATGTTAAACAGCAACCGTAATCCGGATAGTTCGTTGAATCAGCAGCATATACTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGaa                                                                      <  1:702489/140‑1 (MQ=255)                                                               ctgcCCAACACCAACGGCGGGATGTTAAACAGCAACCGTAATCCGGATAGTTCGTTGAATCAGCAGCATATACTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGaa                                                                      <  2:1218154/140‑1 (MQ=255)                                                               ctgcCCAACACCAACGGCGGGATGTTAAACAGCAACCGTAATCCGGATAGTTCGTTGAATCAGCAGCATATACTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGaa                                                                      <  2:1355225/140‑1 (MQ=255)                                                                 gcCCAACACCAACGGCGGGATGTTAAACAGCAACCGTAATCCGGATAGTTCGTTGAATCAGCAGCATATACTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGaaaa                                                                    <  2:332833/140‑1 (MQ=255)                                                                        acCAACGGCGGGATGTTAAACAGCAACCGTAATCCGGATAGTTCGTTGAATCAGCAGCATATACTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAACTATTgg                                                             >  1:402863/1‑140 (MQ=255)                                                                          cAACGGCGGGATGTTAAACAGCAACCGTAATCCGGATAGTTCGTTGAATCAGCAGCATATACTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAACTATTGGGc                                                           >  1:866013/1‑140 (MQ=255)                                                                            aCGGCGGGATGTTAAACAGCAACCGTAATCCGGATAGTTCGTTGAATCAGCAGCATATACTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAACTATTGGGCCg                                                         >  2:547081/1‑140 (MQ=255)                                                                            aCGGCGGGATGTTAAACAGCAACCGTAATCCGGATAGTTCGTTGAATCAGCAGCATATACTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAACTATTGGGCCg                                                         >  2:823851/1‑140 (MQ=255)                                                                            aCGGCGGGATGTTAAACAGCAACCGTAATCCGGATAGTTCGTTGAATCAGCAGCATATACTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAACTATTGGGCCg                                                         >  1:1348232/1‑140 (MQ=255)                                                                             cggcggGATGTTAAACAGCAACCGTAATCCGGATAGTTCGTTGAATCAGCAGCATATACTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAACTATTGGGCCGt                                                        <  1:189626/140‑1 (MQ=255)                                                                              ggcggGATGTTAAACAGCAACCGTAATCCGCATAGTTCGTTGAATCAGCAGCATATACTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAACTATTGGGCCGTa                                                       <  2:866013/140‑1 (MQ=255)                                                                                   gATGTTAAACAGCAACCGTAATCCGGATAGTTCGTTGAATCAGCAGCATATACTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAACTATTGGGCCGTAAAGtt                                                  >  2:1133990/1‑140 (MQ=255)                                                                                     tGTTAAACAGCAACCGTAATCCGGATAGTTCGTTGAATCAGCAGCATATACTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAACTATTGGGCCGTAAAGTTCg                                                <  2:1220397/140‑1 (MQ=255)                                                                                       ttAAACAGCAACCGTAATCCGGATAGTTCGTTGAATCAGCAGCATATACTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAACTATTGGGCCGTAAAGTTCGGa                                              <  2:215892/140‑1 (MQ=255)                                                                                               cAACCGTAATCCGGATAGTTCGTTGAATCAGCAGCATATACTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAACTATTGGGCCGTAAAGTTCGGACCAATCAc                                      >  2:1191614/1‑140 (MQ=255)                                                                                               cAACCGTAATCCGGATAGTTCGTTGAATCAGCAGCATATACTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAACTATTGGGCCGTAAAGTTCGGACCAATCAc                                      >  2:770170/1‑140 (MQ=255)                                                                                               cAACCGTAATCCGGATAGTTCGTTGAATCAGCAGCATATACTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAACTATTGGGCCGTAAAGTTCGGACCAATCAc                                      >  1:79357/1‑140 (MQ=255)                                                                                               cAACCGTAATCCGGATAGTTCGTTGAATCAGCAGCATATACTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAACTATTGGGCCGTAAAGTTCGGACCAATCAc                                      >  1:153385/1‑140 (MQ=255)                                                                                                     tAATGCGGATAGTCCGTTGAACCAGCAGCATATGCTGCCGGAGAGGAGAAACGGGGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAACTATTGGGCCGTAAAGTTCGGGCCAATCACGTCAAt                                <  2:757839/140‑1 (MQ=255)                                                                                                     tAATCCGGATAGTTCGTTGAATCAGCAGCATATACTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAACTATTGGGCCGTAAAGTTCGGACCAATCACGTCAAt                                >  2:1005760/1‑140 (MQ=255)                                                                                                     tAATCCGGATAGTTCGTTGAATCAGCAGCATATACTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAACTATTGGGCCGTAAAGTTCGGACCAATCACGTCAAt                                <  2:1348232/140‑1 (MQ=255)                                                                                                       aTCCGGATAGTTCGTTGAATCAGCAGCATATACTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAACTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCg                              <  2:28560/140‑1 (MQ=255)                                                                                                        tCCGGATAGTTCGTTGAATCAGCAGCATATACTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAACTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGc                             <  2:683453/140‑1 (MQ=255)                                                                                                               aGTTCGTTGAATCAGCAGCATATACTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAACTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTa                      <  2:402863/140‑1 (MQ=255)                                                                                                                 ttCGTTGAATCAGCAGCATATACTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAACTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACa                    >  1:930465/1‑140 (MQ=255)                                                                                                                 ttCGTTGAATCAGCAGCATATACTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAACTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACa                    >  1:134744/1‑140 (MQ=255)                                                                                                                 ttCGTTGAATCAGCAGCATATACTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAACTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACa                    >  2:259069/1‑140 (MQ=255)                                                                                                                    gTTGAATCAGCAGCATATACTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAACTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGAt                 >  1:482977/1‑140 (MQ=255)                                                                                                                     ttGAATCAGCAGCATATACTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAACTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATg                <  2:134744/140‑1 (MQ=255)                                                                                                                       gAATCAGCAGCATATACTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAACTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCa              <  2:339103/140‑1 (MQ=255)                                                                                                                       gAATCAGCAGCATATACTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAACTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCa              <  2:846017/140‑1 (MQ=255)                                                                                                                            agcagcATATACTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAACTATTGGGCCGTAAAGTTCGGACCNATCACGTCAATCGCATCGGTACAGATGCAATCca         <  1:355376/140‑1 (MQ=255)                                                                                                                              cagcaTATACTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAACTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCcaca       >  1:389785/1‑140 (MQ=255)                                                                                                                                   tataCTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAACTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCa  >  1:1366378/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                         ATAATTCTCAGCGGGTGTTGCAGTCACAGCCGGGGGAGCGAAATCCCGCCCGGCAGCAAATGCTGCCCAACACCAACGGCGGGATGTTAAACAGCAACCGTAATCCGGATAGTTCGTTGAATCAGCAGCATATGCTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAACTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCA  >  NC_000913/3587166‑3587435

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑