Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	453,140	G→A	L136F (CTT→TTT)	ampG ←	muropeptide:H(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	453,140	0	G	A	100.0%	282.4 / NA	81	L136F (CTT→TTT)	ampG	muropeptide:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (40/41);  total (40/41)

CGCCATTAACCAGTACATGCCCTGCCAGCCCAGCCATTTATCTGCCAGCCACAGGGCTAGGCCGCCGGAAACCAGCATCCCTAAACGGTAACCCAGCACGCTGATTGCCGCGCCCGCACCACGTTCTTCTGCCGGAAGCACATCGGTTTTCCACGCATCGAAGACTATATCCTGGGAAGCAGAGCAAAAAGCGATCACCACTGCCAGCGCCGCCATCCAGCGGAGTTGGGTGCCTGGTTCGAGAAAACCCATCGCCGCAATGGCGACTAATAAC  >  NC_000913/453003‑453276                                                                                                                                          |                                                                                                                                         cgccATTAACCAGTACATGCCCTGCCAGCCCAGCCATTTATCTGCCAGCCACAGGGCTAGGCCGCCGGAAACCAGCATCCCTAAACGGTAACCCAGCACGCTGATTGCCGCGCCCGCACCACGTTCTTCTGCCGGAAAca                                                                                                                                        >  2:1042254/1‑140 (MQ=255)     aTTAACCAGTACATGCCCTGCCAGCCCAGCCATTTATCTGCCAGCCACAGGGCTAGGCCGCCGGAAACCAGCATCCCTAAACGGTAACCCAGCACGCTGATTGCCGCGCCCGCACCACGTTCTTCTGCCGGAAACACATc                                                                                                                                    >  2:3412/1‑140 (MQ=255)              tACATGCCCTGCCAGCCCAGCCATTTATCTGCCAGCCACAGGGCTAGGCCGCCGGAAACCAGCATCCCTAAACGGTAACCCAGCACGCTGATTGCCGCGCCCGCACCACGTTCGTCTGCCGGAAACACATCGGTGTTCCa                                                                                                                           >  2:1250643/1‑140 (MQ=255)               aCATGCCCTGCCAGCCCAGCCATTTATCTGCCAGCCACAGGGCTAGGCCGCCGGAAACCAGCATCCCTAAACGGTAACCCAGCACGCTGATTGCCGCGCCCGCACCACGTTCTTCTGCCGGAAACACATCGGTTTTCCAc                                                                                                                          <  1:669250/140‑1 (MQ=255)               aCATGCCCTGCCAGCCCAGCCATTTATCTGCCAGCCACAGGGCTAGGCCGCCGGAAACCAGCATCCCTAAACGGTAACCCAGCACGCTGATTGCCGCGCCCGCACCACGTTCTTCTGCCGGAAACACATCGGTTTTCCAc                                                                                                                          <  2:957254/140‑1 (MQ=255)               aCATGCCCTGCCAGCCCAGCCATTTATCTGCCAGCCACAGGGCTAGGCCGCCGGAAACCAGCATCCCTAAACGGTAACCCAGCACGCTGATTGCCGCGCCCGCACCACGTTCTTCTGCCGGAAACACATCGGTTTTCCAc                                                                                                                          <  2:632262/140‑1 (MQ=255)                  tGCCCTGCCAGCCCAGCCATTTATCTGCCAGCCACAGGGCTAGGCCGCCGGAAACCAGCATCCCTAAACGGTAACCCAGCACGCTGATTGCCGCGCCCGCACCACGTTCTTCTGCCGGAAACACATCGGTTTTCCACGCa                                                                                                                       <  2:1072963/140‑1 (MQ=255)                   gCCCTGCCAGCCCAGCCATTTATCTGCCAGCCACAGGGCTAGGCCGCCGGAAACCAGCATCCCTAAACGGTAACCCAGCACGCTGATTGCCGCGCCCGCACCACGTTCTTCTGCCGGAAACACATCGGTTTTCCACGCAt                                                                                                                      >  1:700959/1‑140 (MQ=255)                   gCCCTGCCAGCCCAGCCATTTATCTGCCAGCCACAGGGCTAGGCCGCCGGAAACCAGCATCCCTAAACGGTAACCCAGCACGCTGATTGCCGCGCCCGCACCACGTTCTTCTGCCGGAAACACATCGGTTTTCCACGCAt                                                                                                                      >  1:648672/1‑140 (MQ=255)                   gCCCTGCCAGCCCAGCCATTTATCTGCCAGCCACAGGGCTAGGCCGCCGGAAACCAGCATCCCTAAACGGTAACCCAGCACGCTGATTGCCGCGCCCGCACCACGTTCTTCTGCCGGAAACACATCGGTTTTCCACGCAt                                                                                                                      >  2:173673/1‑140 (MQ=255)                   gCCCTGCCAGCCCAGCCATTTATCTGCCAGCCACAGGGCTAGGCCGCCGGAAACCAGCATCCCTAAACGGTAACCCAGCACGCTGATTGCCGCGCCCGCACCACGTTCTTCTGCCGGAAACACATCGGTTTTCCACGCAt                                                                                                                      >  1:18064/1‑140 (MQ=255)                    cccTGCCAGCCCAGCCATTTATCTGCCAGCCACAGGGCTAGGCCGCCGGAAACCAGCATCCCTAAACGGTAACCCAGCACGCTGATTGCCGCGCCCGCACCACGTTCTTCTGCCGGAAACACATCGGTTTTCCACGCATc                                                                                                                     <  2:1322390/140‑1 (MQ=255)                    cccTGCCAGCCCAGCCATTTATCTGCCAGCCACAGGGCTAGGCCGCCGGAAACCAGCATCCCTAAACGGTAACCCAGCACGCTGATTGCCGCGCCCGCACCACGTTCTTCTGCCGGAAACACATCGGTTTTCCACGCATc                                                                                                                     >  1:1322390/1‑140 (MQ=255)                    cccTGCCAGCCCAGCCATTTATCTGCCAGCCACAGGGCTAGGCCGCCGGAAACCAGCATCCCTAAACGGTAACCCAGCACGCTGATTGCCGCGCCCGCACCACGTTCTTCTGCCGGAAACACATCGGTTTTCCACGCATc                                                                                                                     >  1:433764/1‑140 (MQ=255)                     ccTGCCAGCCCAGCCATTTATCTGCCAGCCACAGGGCTAGGCCGCCGGAAACCAGCATCCCTAAACGGTAACCCAGCACGCTGATTGCCGCGCCCGCACCACGTTCTTCTGCCGGAAACACATCGGTTTTCCACGCATCg                                                                                                                    <  2:1235147/140‑1 (MQ=255)                     ccTGCCAGCCCAGCCATTTATCTGCCAGCCACAGGGCTAGGCCGCCGGAAACCAGCATCCCTAAACGGTAACCCAGCACGCTGATTGCCGCGCCCGCACCACGTTCTTCTGCCGGAAACACATCGGTTTTCCACGCATCg                                                                                                                    >  1:349644/1‑140 (MQ=255)                     ccTGCCAGCCCAGCCATTTATCTGCCAGCCACAGGGCTAGGCCGCCGGAAACCAGCATCCCTAAACGGTAACCCAGCACGCTGATTGCCGCGCCCGCACCACGTTCTTCTGCCGGAAACACATCGGTTTTCCACGCATCg                                                                                                                    <  1:188645/140‑1 (MQ=255)                      cTGCCAGCCCAGCCATTTATCTGCCAGCCACAGGGCTAGGCCGCCGGAAACCAGCATCCCTAAACGGTAACCCAGCACGCTGATTGCCGCGCCCGCACCACGTTCTTCTGCCGGAAACACATTGGTTTTCCACGCATCGa                                                                                                                   <  1:115170/140‑1 (MQ=255)                      cTGCCAGCCCAGCCATTTATCTGCCAGCCACAGGGCTAGGCCGCCGGAAACCAGCATCCCTAAACGGTAACCCAGCACGCTGATTGCCGCGCCCGCACCACGTTCTTCTGCCGGAAACACATCGGTTTTCCACGCATCGa                                                                                                                   <  1:932300/140‑1 (MQ=255)                      cTGCCAGCCCAGCCATTTATCTGCCAGCCACAGGGCTAGGCCGCCGGAAACCAGCATCCCTAAACGGTAACCCAGCACGCTGATTGCCGCGCCCGCACCACGTTCTTCTGCCGGAAACACATCGGTTTTCCACGCATCGa                                                                                                                   <  2:54773/140‑1 (MQ=255)                      cTGCCAGCCCAGCCATTTATCTGCCAGCCACAGGGCTAGGCCGCCGGAAACCAGCATCCCTAAACGGTAACCCAGCACGCTGATTGCCGCGCCCGCACCACGTTCTTCTGCCGGAAACACATCGGTTTTCCACGCATCGa                                                                                                                   <  1:173673/140‑1 (MQ=255)                      cTGCCAGCCCAGCCATTTATCTGCCAGCCACAGGGCTAGGCCGCCGGAAACCAGCATCCCTAAACGGTAACCCAGCACGCTGATTGCCGCGCCCGCACCACGTTCTTCTGCCGGAAACACATCGGTTTTCCACGCATCGa                                                                                                                   <  1:596008/140‑1 (MQ=255)                      cTGCCAGCCCAGCCATTTATCTGCCAGCCACAGGGCTAGGCCGCCGGAAACCAGCATCCCTAAACGGTAACCCAGCACGCTGATTGCCGCGCCCGCACCACGTTCTTCTGCCGGAAACACATCGGTTTTCCACGCATCGa                                                                                                                   <  1:842749/140‑1 (MQ=255)                      cTGCCAGCCCAGCCATTTATCTGCCAGCCACAGGGCTAGGCCGCCGGAAACCAGCATCCCTAAACGGTAACCCAGCACGCTGATTGCCGCGCCCGCACCACGTTCTTCTGCCGGAAACACATCGGTTTTCCACGCATCGa                                                                                                                   <  1:1250643/140‑1 (MQ=255)                      cTGCCAGCCCAGCCATTTATCTGCCAGCCACAGGGCTAGGCCGCCGGAAACCAGCATCCCTAAACGGTAACCCAGCACGCTGATTGCCGCGCCCGCACCACGTTCTTCTGCCGGAAACACATCGGTTTTCCACGCATCGa                                                                                                                   <  2:414269/140‑1 (MQ=255)                       tGCCAGCCCAGCCATTTATCTGCCAGCCACAGGGCTAGGCCGCCGGAAACCAGCATCCCTAAACGGTAACCCAGCACGCTGATTGCCGCGCCCGCACCACGTTCTTCTGCCGGAAACACATCGGTTTTCCa                                                                                                                           >  2:1081242/1‑131 (MQ=255)                       tGCCAGCCCAGCCATTTATCTGCCAGCCACAGGGCTAGGCCGCCGGAAACCAGCATCCCTAAACGGTAACCCAGCACGCTGATTGCCGCGCCCGCACCACGTTCTTCTGCCGGAAACACATCGGTTTTCCa                                                                                                                           <  1:1081242/131‑1 (MQ=255)                        gCCAGCCCAGCCATTTATCTGCCAGCCACAGGGCTAGGCCGCCGGAAACCAGCATCCCTAAACGGTAACCCAGCACGCTGATTGCCGCGCCCGCACCACGTTCTTCTGCCGGAAACACATCGGTTTTCCACGCATCGAAg                                                                                                                 >  2:429456/1‑140 (MQ=255)                        gCCAGCCCAGCCATTTATCTGCCAGCCACAGGGCTAGGCCGCCGGAAACCAGCATCCCTAAACGGTAACCCAGCACGCTGATTGCCGCGCCCGCACCACGTTCTTCTGCCGGAAACACATCGGTTTTCCACGCATCGAAg                                                                                                                 >  2:355786/1‑140 (MQ=255)                        gCCAGCCCAGCCATTTATCTGCCAGCCACAGGGCTAGGCCGCCGGAAACCAGCATCCCTAAACGGTAACCCAGCACGCTGATTGCCGCGCCCGCACCACGTTCTTCTGCCGGAAACACATCGGTTTTCCACGCATCGAAg                                                                                                                 >  2:398282/1‑140 (MQ=255)                        gCCAGCCCAGCCATTTATCTGCCAGCCACAGGGCTAGGCCGCCGGAAACCAGCATCCCTAAACGGTAACCCAGCACGCTGATTGCCGCGCCCGCACCACGTTCTTCTGCCGGAAACACATCGGTTTTCCACGCATCGAAg                                                                                                                 >  1:70913/1‑140 (MQ=255)                         ccagcccagcCATTTATCTGCCAGCCACAGGGCTAGGCCGCCGGAAACCAGCATCCCTAAACGGTAACCCAGCACGCTGATTGCCGCGCCCGCACCACGTTCTTCTGCCGGAAACACATCGGTTTTCCACGCATCGAAGa                                                                                                                <  2:263255/140‑1 (MQ=255)                           agcccagccATTTATCTGCCAGCCACAGGGCTAGGCCGCCGGAAACCAGCATCCCTAAACGGTAACCCAGCACGCTGATTGCCGCGCCCGCACCACGTTCTTCTGCCGGAAACACATCGGTTTTCCACGCATCGAAGACt                                                                                                              <  2:700959/140‑1 (MQ=255)                           agcccagccATTTATCTGCCAGCCACAGGGCTAGGCCGCCGGAAACCAGCATCCCTAAACGGTAACCCAGCACGCTGATTGCCGCGCCCGCACCACGTTCTTCTGCCGGAAACACATCGGTTTTCCACGCATCGAAGACt                                                                                                              <  2:1380654/140‑1 (MQ=255)                           agcccagccATTTATCTGCCAGCCACAGGGCTAGGCCGCCGGAAACCAGCATCCCTAAACGGTAACCCAGCACGCTGATTGCCGCGCCCGCACCACGTTCTTCTGCCGGAAACACATCGGTTTTCCACGCATCGAAGACt                                                                                                              <  2:756533/140‑1 (MQ=255)                           agcccagccATTTATCTGCCAGCCACAGGGCTAGGCCGCCGGAAACCAGCATCCCTAAACGGTAACCCAGCACGCTGATTGCCGCGCCCGCACCACGTTCTTCTGCCGGAAACACATCGGTTTTCCACGCATCGAAGACt                                                                                                              <  2:943147/140‑1 (MQ=255)                              ccagccATTTATCTGCCAGCCACAGGGCTAGGCCGCCGGAAACCAGCATCCCTAAACGGTAACCCAGCACGCTGATTGCCGCGCCCGCACCACGTTCTTCTGCCGGAAACACATCGGTTTTCCACGCATCGAAGACtata                                                                                                           >  2:746252/1‑140 (MQ=255)                                  ccATTTATCTGCCAGCCACAGGGCTAGGCCGCCGGAAACCAGCATCCCTAAACGGTAACCCAGCACGCTGATTGCCGCGCCCGCACCACGTTCTTCTGCCGGAAACACATCGGTTTTCCACGCATCg                                                                                                                    <  2:282060/127‑1 (MQ=255)                                  ccATTTATCTGCCAGCCACAGGGCTAGGCCGCCGGAAACCAGCATCCCTAAACGGTAACCCAGCACGCTGATTGCCGCGCCCGCACCACGTTCTTCTGCCGGAAACACATCGGTTTTCCACGCATCg                                                                                                                    >  1:282060/1‑127 (MQ=255)                                        aTCTGCCAGCCACAGGGCTAGGCCGCCGGAAACCAGCATCCCTAAACGGTAACCCAGCACGCTGATTGCCGCGCCCGCACCACGTTCTTCTGCCGGAAACACATCGGTTTTCCACGCATCGa                                                                                                                   >  1:71332/1‑122 (MQ=255)                                        aTCTGCCAGCCACAGGGCTAGGCCGCCGGAAACCAGCATCCCTAAACGGTAACCCAGCACGCTGATTGCCGCGCCCGCACCACGTTCTTCTGCCGGAAACACATCGGTTTTCCACGCATCGa                                                                                                                   <  2:71332/122‑1 (MQ=255)                                           tGCCAGCCACAGGGCTAGGCCGCCGGAAACCAGCATCCCTAAACGGTAACCCAGCACGCTGATTGCCGCGCCCGCACCACGTTCTTCTGCCGGAAACACATCGGTTTTCCACGCATCGAAGACTATATCCTGGGAAGCag                                                                                              <  1:3412/140‑1 (MQ=255)                                            gccagccaCAGGGCTAGGCCGCCGGAAACCAGCATCCCTAAACGGTAACCCAGCACGCTGATTGCCGCGCCCGCACCACGTTCTTCTGCCGGAAACACATCGGTTTTCCACGCATCGAAGACTATATCCTGGGAAGCaga                                                                                             >  1:1082205/1‑140 (MQ=255)                                                gccaCAGGGCTAGGCCGCCGGAAACCAGCATCCCTAAACGGTAACCCAGCACGCTGATTGCCGCGCCCGCACCACGTTCTTCTGCCGGAAACACATCGGTTTTCCACGCATCGAAGACTATATCCTGGGAAGCAGAGCaa                                                                                         >  2:1339090/1‑140 (MQ=255)                                                    caGGGCTAGGCCGCCGGAAACCAGCATCCCTAAACGGTAACCCAGCACGCTGATTGCCGCGCCCGCACCACGTTCTTCTGCCGGAAACACATCGGTTTTCCACGCATCGAAGACTATATCCTGGGAAGCAGAGCAAAAAg                                                                                     >  2:994555/1‑140 (MQ=255)                                                       ggCTAGGCCGCCGGAAACCAGCATCCCTAAACGGTAACCCAGCACGCTGATTGCCGCGCCCGCACCACGTTCTTCTGCCGGAAACACATCGGTTTTCCACGCATCGAAGACTATATCCTGGGAAGCAGAGCAAAAAGCGa                                                                                  <  1:334383/140‑1 (MQ=255)                                                       ggCTAGGCCGCCGGAAACCAGCATCCCTAAACGGTAACCCAGCACGCTGATTGCCGCGCCCGCACCACGTTCTTCTGCCGGAAACACATCGGTTTTCCACGCATCGAAGACTATATCCTGGGAAGCAGAGCAAAAAGCGa                                                                                  <  1:994555/140‑1 (MQ=255)                                                       ggCTAGGCCGCCGGAAACCAGCATCCCTAAACGGTAACCCAGCACGCTGATTGCCGCGCCCGCACCACGTTCTTCTGCCGGAAACACATCGGTTTTCCACGCATCGAAGACTATATCCTGGGAAGCAGAGCAAAAAGCGa                                                                                  <  2:648672/140‑1 (MQ=255)                                                       ggCTAGGCCGCCGGAAACCAGCATCCCTAAACGGTAACCCAGCACGCTGATTGCCGCGCCCGCACCACGTTCTTCTGCCGGAAACACATCGGTTTTCCACGCATCGAAGACTATATCCTGGGAAGCAGAGCAAAAAGCGa                                                                                  <  1:82420/140‑1 (MQ=255)                                                       ggCTAGGCCGCCGGAAACCAGCATCCCTAAACGGTAACCCAGCACGCTGATTGCCGCGCCCGCACCACGTTCTTCTGCCGGAAACACATCGGTTTTCCACGCATCGAAGACTATATCCTGGGAAGCAGAGCAAAAAGCGa                                                                                  <  1:746252/140‑1 (MQ=255)                                                            ggCCGCCGGAAACCAGCATCCCTAAACGGTAACCCAGCACGCTGATTGCCGCGCCCGCACCACGTTCTTCTGCCGGAAACACATCGGTTTTCCACGCATCGAAGACTATATCCTGGGAAGCAGAGCAAAAAGCGATcacc                                                                             >  1:902029/1‑140 (MQ=255)                                                                 ccgGAAACCAGCATCCCTAAACGGTAACCCAGCACGCTGATTGCCGCGCCCGCACCACGTTCTTCTGCCGGAAACACATCGGTTTTCCACGCATCGAAGACTATATCCTGGGAAGCAGAGCAAAAAGCGATCACCACTGc                                                                        >  1:1156200/1‑140 (MQ=255)                                                                 ccgGAAACCAGCATCCCTAAACGGTAACCCAGCACGCTGATTGCCGCGCCCGCACCACGTTCTTCTGCCGGAAACACATCGGTTTTCCACGCATCGAAGACTATATCCTGGGAAGCAGAGCAAAAAGCGATCACCACTGc                                                                        >  1:344938/1‑140 (MQ=255)                                                                 cccgAAACCAGCATCCCTAAACGGTAACCCAGCACGCTGATTGCCGCGCCCGCACCACGTTCTTCTGCCGGAAACACATCGGTTTTCCACGCATCGAAGACTATATCCTGGGAAGCAGAGCAAAAAGCGATCACCACTGc                                                                        >  2:323844/4‑140 (MQ=255)                                                                         cAGCATCCCTAAACGGTAACCCAGCACGCTGATTGCCGCGCCCGCACCACGTTCTTCTGCCGGAAACACATCGGTTTTCCACGCATCGAAGACTATATCCTGGGAAGCAGAGCAAAAAGCGATCACCACTGCCAGcgccg                                                                <  1:355786/140‑1 (MQ=255)                                                                              tCCCTAAACGGTAACCCAGCACGCTGATTGCCGCGCCCGCACCACGTTCTTCTGCCGGAAACACATCGGTTTTCCACGCATCGAAGACTATATCCTGGGAAGCAGAGCAAAAAGCGATCACCACTGCCAGCGCCGCCATc                                                           <  2:198518/140‑1 (MQ=255)                                                                                      cGGTAACCCAGCACGCTGATTGCCGCGCCCGCACCACGTTCTTCTGCCGGAAACACATCGGTTTTCCACGCATCGAAGACTATATCCTGGGAAGCAGAGCAAAAAGCGATCACCACTGCCAGCGCCGCCATCCAGCGGAg                                                   >  2:118199/1‑140 (MQ=255)                                                                                         tAACCCAGCACGCTGATTGCCGCGCCCGCACCACGTTCTTCTGCCGGAAACACATCGGTTTTCCACGCATCGAAGACTATATCCTGGGAAGCAGAGCAAAAAGCGATCACCACTGCCAGCGCCGCCATCCAGCGGAGTTg                                                >  2:374215/1‑140 (MQ=255)                                                                                         tAACCCAGCACGCTGATTGCCGCGCCCGCACCACGTTCTTCTGCCGGAAACACATCGGTTTTCCACGCATCGAAGACTATATCCTGGGAAGCAGAGCAAAAAGCGATCACCACTGCCAGCGCCGCCATCCAGCGGAGTTg                                                >  1:1310373/1‑140 (MQ=255)                                                                                         tAACCCAGCACGCTGATTGCCGCGCCCGCACCACGTTCTTCTGCCGGAAACACATCGGTTTTCCACGCATCGAAGACTATATCCTGGGAAGCAGAGCAAAAAGCGATCACCACTGCCAGCGCCGCCATCCAGCGGAGTTg                                                >  2:495980/1‑140 (MQ=255)                                                                                         tAACCCAGCACGCTGATTGCCGCGCCCGCACCACGTTCTTCTGCCGGAAACACATCGGTTTTCCACGCATCGAAGACTATATCCTGGGAAGCAGAGCAAAAAGCGATCACCACTGCCAGCGCCGCCATCCAGCGGAGTTg                                                >  1:655036/1‑140 (MQ=255)                                                                                         tAACCCAGCACGCTGATTGCCGCGCCCGCACCACGTTCTTCTGCCGGAAACACATCGGTTTTCCACGCATCGAAGACTATATCCTGGGAAGCAGAGCAAAAAGCGATCACCACTGCCAGCGCCGCCATCCAGCGGAGTTg                                                >  2:830668/1‑140 (MQ=255)                                                                                          aaCCCAGCACGCTGATTGCCGCGCCCGCACCACGTTCTTCTGCCGGAAACACATCGGTTTTCCACGCATCGAAGACTATATCCTGGGAAGCAGAGCAAAAAGCGATCACCACTGCCAGCGCCGCCATCCAGCGGAGTTgg                                               >  2:361670/1‑140 (MQ=255)                                                                                           aCCCAGCACGCTGATTGCCGCGCCCGCACCACGTTCTTCTGCCGGAAACACATCGGTTTTCCACGCATCGAAGACTATATCCTGGGAAGCAGAGCAAAAAGCGATCACCACTGCCAGCGCCGCCATCCAGCGGAGTTggg                                              >  2:316989/1‑140 (MQ=255)                                                                                                   cGCTGATTGCCGCGCCCGCACCACGTTCTTCTGCCGGAAACACATCGGTTTTCCACGCATCGAAGACTATATCCTGGGAAGCAGAGCAAAAAGCGATCACCACTGCCAGCGCCGCCATCCAGCGGAGTTGGGTGCCTGGt                                      >  2:772706/1‑140 (MQ=255)                                                                                                    gCTGATTGCCGCGCCCGCACCACGTTCTTCTGCCGGAAACACATCGGTTTTCCACGCATCGAAGACTATATCCTGGGAAGCAGAGCAAAAAGCGATCACCACTGCCAGCGCCGCCATCCAGCGGAGTTGGGTGCCTGGtt                                     <  1:1339090/140‑1 (MQ=255)                                                                                                    gCTGATTGCCGCGCCCGCACCACGTTCTTCTGCCGGAAACACATCGGTTTTCCACGCATCGAAGACTATATCCTGGGAAGCAGAGCAAAAAGCGATCACCACTGCCAGCGCCGCCATCCAGCGGAGTTGGGTGCCTGGtt                                     >  2:768658/1‑140 (MQ=255)                                                                                                     cTGATTGCCGCGCCCGCACCACGTTCTTCTGCCGGAAACACATCGGTTTTCCACGCATCGAAGACTATATCCTGGGAAGCAGAGCAAAAAGCGATCACCACTGCCAGCGCCGCCATCCAGCGGAGTTGGGTGCCTGGTTc                                    >  2:929316/1‑140 (MQ=255)                                                                                                       gATTGCCGCGCCCGCACCACGTTCTTCTGCCGGAAACACATCGGTTTTCCACGCATCGAAGACTATATCCTGGGAAGCAGAGCAAAAAGCGATCACCACTGCCAGCGCCGCCATCCAGCGGAGTTGGGTGCCTGGTTCga                                  >  2:1090362/1‑140 (MQ=255)                                                                                                         ttGCCGCGCCCGCACCACGTTCTTCTGCCGGAAACACATCGGTTTTCCACGCATCGAAGACTATATCCTGGGAAGCAGAGCAAAAAGCGATCACCACTGCCAGCGCCGCCATCCAGCGGAGTTGGGTGCCTGGTTCgaga                                <  2:344938/140‑1 (MQ=255)                                                                                                          tGCCGCGCCCGCACCACGTTCTTCTGCCGGAAACACATCGGTTTTCCACGCATCGAAGACTATATCCTGGGAAGCAGAGCAAAAAGCGATCACCACTGCCAGCGCCGCCATCCAGCGGAGTTGGGTGCCTGGTTCGAGaa                               <  1:374215/140‑1 (MQ=255)                                                                                                                gcCCGCACCACGTTCTTCTGCCGGAAACACATCGGTTTTCCACGCATCGAAGACTATATCCTGGGAAGCAGAGCAAAAAGCGATCACCACTGCCAGCGCCGCCATCCAGCGGAGTTGGGTGCCTGGTTCGAGAAAACCCa                         >  2:724938/1‑140 (MQ=255)                                                                                                                 cccGCACCACGTTCTTCTGCCGGAAACACATCGGTTTTCCACGCATCGAAGACTATATCCTGGGAAGCAGAGCAAAAAGCGATCACCACTGCCAGCGCCGCCATCCAGCGGAGTTGGGTGCCTGGTTCGAGAAAACCCAt                        <  2:433764/140‑1 (MQ=255)                                                                                                                      accacGTTCTTCTGCCGGAAACACATCGGTTTTCCACGCATCGAAGACTATATCCTGGGAAGCAGAGCAAAAAGCGATCACCACTGCCAGCGCCGCCATCCAGCGGAGTTGGGTGCCTGGTTCGAGAAAACCCATcgccg                   >  1:1163543/1‑140 (MQ=255)                                                                                                                          cGTTCTTCTGCCGGAAACACATCGGTTTTCCACGCATCGAAGACTATATCCTGGGAAGCAGAGCAAAAAGCGATCACCACTGCCAGCGCCGCCATCCAGCGGAGTTGGGTGCCTGGTTCGAGAAAACCCATCGCCGCAAt               >  1:609342/1‑140 (MQ=255)                                                                                                                            ttcttcTGCCGGAAACACATCGGTTTTCCACGCATCGAAGACTCTATCCTGGGAAGCAGAGCAAAAAGCGATCACCACTGCCAGCGCCGCCATCCAGCGGAGTTGGGTGCCTGGTTCGAGAAAACCCATCGCCGCCAttg             <  2:1310373/140‑3 (MQ=255)                                                                                                                            ttcttcTGCCGGAAACACATCGGTTTTCCACGCATCGAAGACTATATCCTGGGAAGCAGAGCAAAAAGCGATCACCACTGCCAGCGCCGCCATCCAGCGGAGTTGGGTGCCTGGTTCGAGAAAACCCATCGCCGCAATgg             <  2:918017/140‑1 (MQ=255)                                                                                                                            ttcttcTGCCGGAAACACATCGGTTTTCCACGCATCGAAGACTATATCCTGGGAAGCAGAGCAAAAAGCGATCACCACTGCCAGCGCCGCCATCCAGCGGAGTTGGGTGCCTGGTTCGAGAAAACCCATCGCCGCAATgg             <  2:1082205/140‑1 (MQ=255)                                                                                                                                tctGCCGGAAACACATCGGTTTTCCACGCATCGAAGACTATATCCTGGGAAGCAGAGCAAAAAGCGATCACCACTGCCAGCGCCGCCATCCAGCGGAGTTGGGTGCCTGGTTCGAGAAAACCCATCGCCGCAATGGCGAc         <  1:724938/140‑1 (MQ=255)                                                                                                                                tctGCCGGAAACACATCGGTTTTCCACGCATCGAAGACTATATCCTGGGAAGCAGAGCAAAAAGCGATCACCACTGCCAGCGCCGCCATCCAGCGGAGTTGGGTGCCTGGTTCGAGAAAACCCATCGCCGCAATGGCGAc         <  1:1090362/140‑1 (MQ=255)                                                                                                                                    ccGGAAACACATCGGTTTTCCACGCATCGAAGACTATATCCTGGGAAGCAGAGCAAAAAGCGATCACCACTGCCAGCGCCGCCATCCAGCGGAGTTGGGTGCCTGGTTCGAGAAAACCCATCGCCGCAATGGCGACtaat     >  2:119971/1‑140 (MQ=255)                                                                                                                                       gAAACACATCGGTTTTCCACGCATCGAAGACTATATCCTGGGAAGCAGAGCAAAAAGCGATCACCACTGCCAGCGCCGCCATCCAGCGGAGTTGGGTGCCTGGTTCGAGAAAACCCATCGCCGCAATGGCGACTAATAAc  <  2:1163543/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                         CGCCATTAACCAGTACATGCCCTGCCAGCCCAGCCATTTATCTGCCAGCCACAGGGCTAGGCCGCCGGAAACCAGCATCCCTAAACGGTAACCCAGCACGCTGATTGCCGCGCCCGCACCACGTTCTTCTGCCGGAAGCACATCGGTTTTCCACGCATCGAAGACTATATCCTGGGAAGCAGAGCAAAAAGCGATCACCACTGCCAGCGCCGCCATCCAGCGGAGTTGGGTGCCTGGTTCGAGAAAACCCATCGCCGCAATGGCGACTAATAAC  >  NC_000913/453003‑453276

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑