Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,989,755	A→G	V25A (GTG→GCG)	hemD ←	uroporphyrinogen‑III synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,989,755	0	A	G	100.0%	285.0 / NA	81	V25A (GTG→GCG)	hemD	uroporphyrinogen‑III synthase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (26/55);  total (26/55)

CTGTGATTGGGCAAAAGCAACCGCGTGTTGCGAGAGGGCAAACAACAGATCGCTCTCCCCCAGCGCTGCCAGTTGATCAGCAAGTTGCGGTAATTGTTGACCCGGAGAAAACTCAATCAGCGGAAAATGCCAGGCCACCTGCCCCAGTGTGCGCAGACGGCTCACTAACTCTTCTCCAGCGGGAGACGGGCGGGTGACAAGGATACTCATGCCGGGGCGTCTCCGTTATAGACTTCAGCGAGGATCTCGCGCGCGCCGTTATTCAGTA  >  NC_000913/3989619‑3989886                                                                                                                                         |                                                                                                                                    cTGTGATTGGGCAAAAGCAACCGCGTGTTGCGAGAGGGCAAACAACAGATCGCTCTCCCCCAGCGCTGCCAGTTGATCAGCAAGTTGCGGTAATTGTTGACCCGGAGAAAACTCAATCAGCGGAAAATGCCAGGCCGCCt                                                                                                                                  >  1:1061819/1‑140 (MQ=255) cTGTGATTGGGCAAAAGCAACCGCGTGTTGCGAGAGGGCAAACAACAGATCGCTCTCCCCCAGCGCTGCCAGTTGATCAGCAAGTTGCGGTAATTGTTGACCCGGAGAAAACTCAATCAGCGGAAAATGCCAGGCCGCCt                                                                                                                                  >  2:1393902/1‑140 (MQ=255)    tgATTGGGCAAAAGCAACCGCGTGTTGCGAGAGGGCAAACAACAGATCGCTCTCCCCCAGCGCTGCCAGTTGATCAGCAAGTTGCGGTAATTGTTGACCCGGAGAAAACTCAATCAGCGGAAAATGCCAGGCCGCCTGcc                                                                                                                               <  1:1058032/140‑1 (MQ=255)    tgATTGGGCAAAAGCAACCGCGTGTTGCGAGAGGGCAAACAACAGATCGCTCTCCCCCAGCGCTGCCAGTTGATCAGCAAGTTGCGGTAATTGTTGACCCGGAGAAAACTCAATCAGCGGAAAATGCCAGGCCGCCTGcc                                                                                                                               <  1:288492/140‑1 (MQ=255)      aTTGGGCAAAAGCAACCGCGTGTTGCGAGAGGGCAAACAACAGATCGCTCTCCCCCAGCGCTGCCAGTTGATCAGCAAGTTGCGGTAATTGTTGACCCGGAGAAAACTCAATCAGCGGAAAATGCCAGGCCGCCTGcccc                                                                                                                             >  2:890963/1‑140 (MQ=255)      aTTGGGCAAAAGCAACCGCGTGTTGCGAGAGGGCAAACAACAGATCGCTCTCCCCCAGCGCTGCCAGTTGATCAGCAAGTTGCGGTAATTGTTGACCCGGAGAAAACTCAATCAGCGGAAAATGCCAGGCCGCCTGcccc                                                                                                                             <  2:216739/140‑1 (MQ=255)        tGGGCAAAAGCAACCGCGTGTTGCGAGAGGGCAAACAACAGATCGCTCTCCCCCAGCGCTGCCAGTTGATCAGCAAGTTGCGGTAATTGTTGACCCGGAGAAAACTCAATCAGCGGAAAATGCCAGGCCGCCTGCCCCAg                                                                                                                           <  2:506410/140‑1 (MQ=255)        tGGGCAAAAGCAACCGCATGTTGCGAGAGGGCAAACAACAGATCGCTCTCCCCCAGCGCTGCCAGTTGATCAGCAAGTTGCGGTAATTGTTGACCCGGAGAAAACTCAATCAGCGGAAAATGCCAGGCCGCCTGCCCCAg                                                                                                                           <  1:1179814/140‑1 (MQ=255)         gggCAAAAGCAACCGCGTGTTGCGAGAGGGCAAACAACAGATCGCTCTCCCCCAGCGCTGCCAGTTGATCAGCAAGTTGCGGTAATTGTTGACCCGGAGAAAACTCAATCAGCGGAAAATGCCAGGCCGCCTGCCCCAgg                                                                                                                          >  1:970596/1‑139 (MQ=255)              aaaGCAACCGCGTGTTGCGAGAGGGCAAACAACAGATCGCTCTCCCCCAGCGCTGCCAGTTGATCAGCAAGTTGCGGTAATTGTTGACCCGGAGAAAACTCAATCAGCGGAAAATGCCCGGCCGCCTGCCCCAGTGTgcg                                                                                                                     >  1:514332/1‑140 (MQ=255)                aGCAACCGCGTGTTGCGAGAGGGCAAACAACAGATCGCTCTCCCCCAGCGCTGCCAGTTGATCAGCAAGTTGCGGTAATTGTTGACCCGGAGAAAACTCAATCAGCGGAAAATGCCAGGCCGCCTGCCCCAGTGTGCGCa                                                                                                                   >  1:900331/1‑140 (MQ=255)                    aCCGCGTGTTGCGAGAGGGCAAACAACAGATCGCTCTCCCCCAGCGCTGCCAGTTGATCAGCAAGTTGCGGTAATTGTTGACCCGGAGAAAACTCAATCAGCGGAAAATGCCAGGCCGCCTGCCCCAGTGTGCGCAGAc                                                                                                                <  2:1275335/139‑1 (MQ=255)                    aCCGCGTGTTGCGAGAGGGCAAACAACAGATCGCTCTCCCCCAGCGCTGCCAGTTGATCAGCAAGTTGCGGTAATTGTTGACCCGGAGAAAACTCAATCAGCGGAAAATGCCAGGCCGCCTGCCCCAGTGTGCGCAGAc                                                                                                                >  1:1275335/1‑139 (MQ=255)                    aCCGCGTGTTACGAGAGGGCAAACAACAGATCGCTCTCCCCCAGCGCTGCCAGTTGATCAGCAAGTTGCGGTAATTGTTGACCCGGAGAAAACTCAATCAGCGGAAAATGCCAGGCCGCCTGCCCCAGTGTGCGCAGACg                                                                                                               <  2:1224436/140‑1 (MQ=255)                     ccGCGTGTTGCGAGAGGGCAAACAACAGATCGCTCTCCCCCAGCGCTGCCAGTTGATCAGCAAGTTGCGGTAATTGTTGACCCGGAGAAAACTCAATCAGCGGAAAATGCCAGGCCGCCTGCCCCAGTGTGCGCAGACgg                                                                                                              <  2:414050/140‑1 (MQ=255)                     ccGCGTGTTGCGAGAGGGCAAACAACAGATCGCTCTCCCCCAGCGCTGCCAGTTGATCAGCAAGTTGCGGTAATTGTTGACCCGGAGAAAACTCAATCAGCGGAAAATGCCAGGCCGCCTGCCCCAGTGTGCGCAGACgg                                                                                                              <  2:810480/140‑1 (MQ=255)                          tgtTGCGAGAGGGCAAACAACAGATCGCTCTCCCCCAGCGCTGCCAGTTGATCAGCAAGTTGCGGTAATTGTTGACCCGGAGAAAACTCAATCAGCGGAAAATGCCAGGCCGCCTGCCCCAGTGTGCGCAGACGGCTCAc                                                                                                         >  2:1292408/1‑140 (MQ=255)                           gtTGCGAGAGGGCAAACAACAGATCGCTCTCCCCCAGCGCTGCCAGTTGATCAGCAAGTTGCGGTAATTGTTGACCCGGAGAAAACTCAATCAGCGGAAAATGCCCGGCCGCCTGCCCCAGTGTGCGCAGACGGCTCACt                                                                                                        <  2:514332/140‑1 (MQ=255)                           gtTGCGAGAGGGCAAACAACAGATCGCTCTCCCCCAGCGCTGCCAGTTGATCAGCAAGTTGCGGTAATTGTTGACCCGGAGAAAACTCAATCAGCGGAAAATGCCAGGCCGCCTGCCCCAGTGTGCGCAGACGGCTCACt                                                                                                        <  1:1393902/140‑1 (MQ=255)                             tGCGAGAGGGCAAACAACAGATCGCTCTCCCCCAGCGCTGCCAGTTGATCAGCAAGTTGCGGTAATTGTTGACCCGGAGAAAACTCAATCAGCGGAAAATGCCAGGCCGCCTGCCCCAGTGTGCGCAGACGGCTCACTaa                                                                                                      <  1:112829/140‑1 (MQ=255)                               cGAGAGGGCAAACAACAGATCGCTCTCCCCCAGCGCTGCCAGTTGATCAGCAAGTTGCGGTAATTGTTGACCCGGAGAAAACTCAATCAGCGGAAAATGCCAGGCCGCCTGCCCCAGTGTGCGCAGACGGCTCACTAAct                                                                                                    >  2:186287/1‑140 (MQ=255)                               cGAGAGGGCAAACAACAGATCGCTCTCCCCCAGCGCTGCCAGTTGATCAGCAAGTTGCGGTAATTGTTGACCCGGAGAAAACTCAATCAGCGGAAAATGCCAGGCCGCCTGCCCCAGTGTGCGCAGACGGCTCACTAAct                                                                                                    >  2:1116672/1‑140 (MQ=255)                                     ggCAAACAACAGATCGCTCTCCCCCAGCGCTGCCAGTTGATCAGCAAGTTGCGGTAATTGTTGACCCGGAGAAAACTCAATCAGCGGAAAATGCCAGGCCGCCTGCCCCAGTGTGCGCAGACGGCTCACTAACTCTtctc                                                                                              >  2:101260/1‑140 (MQ=255)                                     ggCAAACAACAGATCGCTCTCCCCCAGCGCTGCCAGTTGATCAGCAAGTTGCGGTAATTGTTGACCCGGAGAAAACTCAATCAGCGGAAAATGCCAGGCCGCCTGCCCCAGTGTGCGCAGACGGCTCACTAACTCTtctc                                                                                              <  1:856350/140‑1 (MQ=255)                                      gCAAACAACAGATCGCTCTCCCCCAGCGCTGCCAGTTGATCAGCAAGTTGCGGTAATTGTTGACCCGGAGAAAACTCAATCAGCGGAAAATGCCAGGCCGCCTGCCCCAGTGTGCGCAGACGGCTCACTAACTCTTCTcc                                                                                             >  1:504243/1‑140 (MQ=255)                                       cAAACAACAGATCGCTCTCCCCCAGCGCTGCCAGTTGATCAGCAAGTTGCGGTAATTGTTGACCCGGAGAAAACTCAATCAGCGGAAAATGCCAGGCCGCCTGCCCCAGTGTGCGCAGACGGCTCACTAACTCTTCTCCa                                                                                            >  1:879709/1‑140 (MQ=255)                                        aaaCAACAGATCGCTCTCCCCCAGCGCTGCCAGTTGATCAGCAAGTTGCGGTAATTGTTGACCCGGAGAAAACTCAATCAGCGGAAAATGCCAGGCCGCCTGCCCCAGTGTGCGCAGACGGCTCACTAACTCTTCTCCAg                                                                                           <  1:1321224/140‑1 (MQ=255)                                        aaaCAACAGATCGCTCTCCCCCAGCGCTGCCAGTTGATCAGCAAGTTGCGGTAATTGTTGACCCGGAGAAAACTCAATCAGCGGAAAATGCCAGGCCGCCTGCCCCAGTGTGCGCAGACGGCTCACTAACTCTTCTCCAg                                                                                           <  2:237696/140‑1 (MQ=255)                                               aGATCGCTCTCCCCCAGCGCTGCCAGTTGATCAGCAAGTTGCGGTAATTGTTGACCCGGAGAAAACTCAATCAGCGGAAAATGCCAGGCCGCCTGCCCCAGTGTGCGCAGACGGCTCACTAACtcttc                                                                                                >  2:1159640/1‑128 (MQ=255)                                               aGATCGCTCTCCCCCAGCGCTGCCAGTTGATCAGCAAGTTGCGGTAATTGTTGACCCGGAGAAAACTCAATCAGCGGAAAATGCCAGGCCGCCTGCCCCAGTGTGCGCAGACGGCTCACTAACtcttc                                                                                                <  1:1159640/128‑1 (MQ=255)                                                   cGCTCTCCCCCAGCGCTGCCAGTTGATCAGCAAGTTGCGGTAATTGTTGACCCGGAGAAAACTCAATCAGCGGAAAATGCCAGGCCGCCTGCCCCAGTGTGCGCAGACGGCTCACTAACTCTTCTCCAGCGGGAGAcggg                                                                                <  2:1130128/140‑1 (MQ=255)                                                   cGCTCTCCCCCAGCGCTGCCAGTTGATCAGCAAGTTGCGGTAATTGTTGACCCGGAGAAAACTCAATCAGCGGAAAATGCCAGGCCGCCTGCCCCAGTGTGCGCAGACGGCTCACTAACTCTTCTCCAGCGGGAGAcggg                                                                                <  2:49232/140‑1 (MQ=255)                                                    gCTCTCCCCCAGCGCTGCCAGTTGATCAGCAAGTTGCGGTAATTGTTGACCCGGAGAAAACTCAATCAGCGGAAAATGCCAGGCCGCCTGCCCCAGTGTGCGCAGACGGCTCACTAACTCTTCTCCAGCGGGAGAcgggc                                                                               <  2:787679/140‑1 (MQ=255)                                                     ctctCCCCCAGCGCTGCCAGTTGATCAGCAAGTTGCGGTAATTGTTGACCCGGAGAAAACTCAATCAGCGGAAAATGCCAGGCCGCCTGCCCCAGTGTGCGCAGACGGCTCACTAACTCTTCTCCAGCGGGAGAcgggcg                                                                              <  1:1241488/140‑1 (MQ=255)                                                     ctctCCCCCAGCGCTGCCAGTTGATCAGCAAGTTGCGGTAATTGTTGACCCGGAGAAAACTCAATCAGCGGAAAATGCCAGGCCGCCTGCCCCAGTGTGCGCAGACGGCTCACTAACTCTTCTCCAGCGGGAGAcgggcg                                                                              >  1:111215/1‑140 (MQ=255)                                                     ctctCCCCCAGCGCTGCCAGTTGATCAGCAAGTTGCGGTAATTGTTGACCCGGAGAAAACTCAATCAGCGGAAAATGCCAGGCCGCCTGCCCCAGTGTGCGCAGACGGCTCACTAACTCTTCTCCAGCGGGAGAcgggcg                                                                              <  2:1344551/140‑1 (MQ=255)                                                     ctctCCCCCAGCGCTGCCAGTTGATCAGCAAGTTGCGGTAATTGTTGACCCGGAGAAAACTCAATCAGCGGAAAATGCCAGGCCGCCTGCCCCAGTGTGCGCAGACGGCTCACTAACTCTTCTCCAGCGGGAGAcgggcg                                                                              <  2:742059/140‑1 (MQ=255)                                                      tctcCCCCAGCGCTGCCAGTTGATCAGCAAGTTGCGGTAATTGTTGACCCGGAGAAAACTCAATCAGCGGAAAATGCCAGGCCGCCTGCCCCAGTGTGCGCAGACGGCTCACTAACTCTTCTCCAGCGGGAGAcgggcgg                                                                             <  1:944992/140‑1 (MQ=255)                                                        tcCCCCAGCGCTGCCAGTTGATCAGCAAGTTGCGGTAATTGTTGACCCGGAGAAAACTCAATCAGCGGAAAATGCCAGGCCGCCTGCCCCAGTGTGCGCAGACGGCTCACTAACTCTTCTCCAGCGGGAGACGGGCGGGt                                                                           <  2:900331/140‑1 (MQ=255)                                                        tcCCCCAGCGCTGCCAGTTGATCAGCAAGTTGCGGTAATTGTTGACCCGGAGAAAACTCAATCAGCGGAAAATGCCAGGCCGCCTGCCCCAGTGTGCGCAGACGGCTCACTAACTCTTCTCCAGCGGGAGACGGGCGGGt                                                                           <  2:286032/140‑1 (MQ=255)                                                          ccccAGCGCTGCCAGTTGATCAGCAAGTTGCGGTAATTGTTGACCCGGAGAAAACTCAATCAGCGGAAAATGCCAGGCCGCCTGCCCCAGTGTGCGCAGACGGCTCACTAACTCTTCTCCAGCGGGAGACGGGCGGGTGa                                                                         <  1:1116672/140‑1 (MQ=255)                                                           cccAGCGCTGCCAGTTGATCAGCAAGTTGCGGTAATTGTTGACCCGGAGAAAACTCAATCAGCGGAAAATGCCAGGCCGCCTGCCCCAGTGTGCGCAGACGGCTCACTAACTCTTCTCCAGCGGGAGACGGGCGGGTGAc                                                                        <  1:434799/140‑1 (MQ=255)                                                              aGCGCTGCCAGTTGATCAGCAAGTTGCGGTAATTGTTGACCCGGAGAAAACTCAATCAGCGGAAAATGCCAGGCCGCCTGCCCCAGTGTGCGCAGACGGCTCACTAACTCTTCTCCAGCGGGAGACGGGCGGGTGACAAg                                                                     <  1:186287/140‑1 (MQ=255)                                                               gcgcTGCCAGTTGATCAGCAAGTTGCGGTAATTGTTGACCCGGAGAAAACTCAATCAGCGGAAAATGCCAGGCCGCCTGCCCCAGTGTGCGCAGACGGCTCACTAACTCTTCTCCAGCGGGAGACGGGCGGGTGACAAgg                                                                    <  2:511704/140‑1 (MQ=255)                                                                cgcTGCCAGTTGATCAGCAAGTTGCGGTAATTGTTGACCCGGAGAAAACTCAATCAGCGGAAAATGCCAGGCCGCCTGCCCCAGTGTGCGCAGACGGCTCACTAACTCTTCTCCAGCGGGAGACGGGCGGGTGACAAGGa                                                                   >  1:581272/1‑140 (MQ=255)                                                                cgcTGCCAGTTGATCAGCAAGTTGCGGTAATTGTTGACCCGGAGAAAACTCAATCAGCGGAAAATGCCAGGCCGCCTGCCCCAGTGTGCGCAGACGGCTCACTAACTCTTCTCCAGCGGGAGACGGGCGGGTGACAAGGa                                                                   <  2:1133977/140‑1 (MQ=255)                                                                  cTGCCAGTTGATCAGCAAGTTGCGGTAATTGTTGACCCGGAGAAAACTCAATCAGCGGAAAATGCCAGGCCGCCTGCCCCAGTGTGCGCAGACGGCTCACTAACTCTTCTCCAGCGGGAGACGGGCGGGTGACAAGGATa                                                                 >  1:785307/1‑140 (MQ=255)                                                                               aGCAAGTTGCGGTAATTGTTGACCCGGAGAAAACTCAATCAGCGGAAAATGCCAGGCCGCCTGCCCCAGTGTGCGCAGACGGCTCACTAACTCTTCTCCAGCGGGAGACGGGCGGGTGACAAGGATACTCATGCCGGGGc                                                    <  1:825013/140‑1 (MQ=255)                                                                                 cAAGTTGCGGTAATTGTTGACCCGGAGAAAACTCAATCAGCGGAAAATGCCAGGCCGCCTGCCCCAGTGTGCGCAGACGGCTCACTAACTCTTCTCCAGCGGGAGACGGGCGGGTGACAAGGATACTCATGCCGGGGCGt                                                  <  1:319033/140‑1 (MQ=255)                                                                                   aGTTGCGGTAATTGTTGACCCGGAGAAAACTCAATCAGCGGAAAATGCCAGGCCGCCTGCCCCAGTGTGCGCAGACGGCTCACTAACTCTTCTCCAGCGGGAGACGGGCGGGTGACAAGGATACTCATGCCGGGGCGtct                                                >  2:976738/1‑140 (MQ=255)                                                                                   aGTTGCGGTAATTGTTGACCCGGAGAAAACTCAATCAGCGGAAAATGCCAGGCCGCCTGCCCCAGTGTGCGCAGACGGCTCACTAACTCTTCTCCAGCGGGAGACGGGCGGGTGACAAGGATACTCATGCCGGGGCGtct                                                <  2:634711/140‑1 (MQ=255)                                                                                   aGTTGCGGTAATTGTTGACCCGGAGAAAACTCAATCAGCGGAAAATGCCAGGCCGCCTGCCCCAGTGTGCGCAGACGGCTCACTAACTCTTCTCCAGCGGGAGACGGGCGGGTGACAAGGATACTCATGCCGGGGCGtct                                                <  1:108073/140‑1 (MQ=255)                                                                                    gTTGCGGTAATTGTTGACCCGGAGAAAACTCAATCAGCGGAAAATGCCAGGCCGCCTGCCCCAGTGTGCGCAGACGGCTCACTAACTCTTCTCCAGCGGGAGACGGGCGGGTGACAAGGATACTCATGCCGGGGCGtctc                                               <  1:1292408/140‑1 (MQ=255)                                                                                      tGCGGTAATTGTTGACCCGGAGAAAACTCAATCAGCGGAAAATGCCAGGCCGCCTGCCCCAGTGTGCGCAGACGGCTCACTAACTCTTCTCCAGCGGGAGACGGGCGGGTGACAAGGATACTCATGCCGGGGc                                                    <  2:117977/133‑1 (MQ=255)                                                                                      tGCGGTAATTGTTGACCCGGAGAAAACTCAATCAGCGGAAAATGCCAGGCCGCCTGCCCCAGTGTGCGCAGACGGCTCACTAACTCTTCTCCAGCGGGAGACGGGCGGGTGACAAGGATACTCATGCCGGGGc                                                    >  1:117977/1‑133 (MQ=255)                                                                                      tGCGGTAATTGTTGACCCGGAGAAAACTCAATCAGCGGAAAATGCCAGGCCGCCTGCCCCAGTGTGCGCAGACGGCTCACTAACTCTTCTCCAGCGGGAGACGGGCGGGTGACAAGGATACTCATGCCGGGGCGTCTCCg                                             <  1:494758/140‑1 (MQ=255)                                                                                         ggTAATTGTTGACCCGGAGAAAACTCAATCAGCGGAAAATGCCAGGCCGCCTGCCCCAGTGTGCGCAGACGGCTCACTAACTCTTCTCCAGCGGGAGACGGGCGGGTGACAAGGATACTCATGCCGGGGCGTCTCCGTta                                          >  2:677317/1‑140 (MQ=255)                                                                                             aTTGTTGACCCGGAGAAAACTCAATCAGCGGAAAATGCCAGGCCGCCTGCCCCAGTGTGCGCAGACGGCTCACTAACTCTTCTCCAGCGGGAGACGGGCGGGTGACAAGGATACTCATGCCGGGGCGTCTCCGTTATAGa                                      >  2:1399425/1‑140 (MQ=255)                                                                                              ttgttgACCCGGAGAAAACTCAATCAGCGGAAAATGCCAGGCCGCCTGCCCCAGTGTGCGCAGACGGCTCACTAACTCTTCTCCAGCGGGAGACGGGCGGGTGACAAGGATACTCATGCCGGGGCGTCTCCGTTATAGAc                                     >  2:46257/1‑140 (MQ=255)                                                                                              ttgttgACCCGGAGAAAACTCAATCAGCGGAAAATGCCAGGCCGCCTGCCCCAGTGTGCGCAGACGGCTCACTAACTCTTCTCCAGCGGGAGACGGGCGGGTGACAAGGATACTCATGCCGGGGCGTCTCCGTTATAGAc                                     >  1:547097/1‑140 (MQ=255)                                                                                               tgttgACCCGGAGAAAACTCAATCAGCGGAAAATGCCAGGCCGCCTGCCCCAGTGTGCGCAGACGGCTCACTAACTCTTCTCCAGCGGGAGACGGGCGGGTGACAAGGATACTCATGCCGGGGCGTCTCCGTTATAGACt                                    <  2:1361705/140‑1 (MQ=255)                                                                                                 ttgACCCGGAGAAAACTCAATCAGCGGAAAATGCCAGGCCGCCTGCCCCAGTGTGCGCAGACGGCTCACTAACTCTTCTCCAGCGGGAGACGGGCGGGTGACAAGGATACTCATGCCGGGGCGTCTCCGTTATAGACTTc                                  <  1:46257/140‑1 (MQ=255)                                                                                                  tgACCCGGAGAAAACTCAATCAGCGGAAAATGCCAGGCCGCCTGCCCCAGTGTGCGCAGACGGCTCACTAACTCTTCTCCAGCGGGAGACGGGCGGGTGACAAGGATACTCATGCCGGGGCGTCTCCGTTATAGACTTCa                                 >  2:1129107/1‑140 (MQ=255)                                                                                                   gACCCGGAGAAAACTCAATCAGCGGAAAATGCCAGGCCGCCTGCCCCAGTGTGCGCAGACGGCTCACTAACTCTTCTCCAGCGGGAGACGGGCGGGTGACAAGGATACTCATGCCGGGGCGTCTCCGTTATAGACTTCAg                                <  1:976738/140‑1 (MQ=255)                                                                                                   gACCCGGAGAAAACTCAATCAGCGGAAAATGCCAGGCCGCCTGCCCCAGTGTGCGCAGACGGCTCACTAACTCTTCTCCAGCGGGAGACGGGCGGGTGACAAGGATACTCATGCCGGGGCGTCTCCGTTATAGACTTCAg                                >  1:1190976/1‑140 (MQ=255)                                                                                                    aCCCGGAGAAAACTCAATCAGCGGAAAATGCCAGGCCGCCTGCCCCAGTGTGCGCAGACGGCTCACTAACTCTTCTCCAGCGGGAGACGGGCGGGTGACAAGGATACTCATGCCGGGGCGTCTCCGTTATAGACt                                    <  1:205284/135‑1 (MQ=255)                                                                                                    aCCCGGAGAAAACTCAATCAGCGGAAAATGCCAGGCCGCCTGCCCCAGTGTGCGCAGACGGCTCACTAACTCTTCTCCAGCGGGAGACGGGCGGGTGACAAGGATACTCATGCCGGGGCGTCTCCGTTATAGACt                                    >  2:205284/1‑135 (MQ=255)                                                                                                     cccGGAGAAAACTCAATCAGCGGAAAATGCCAGGCCGCCTGCCCCAGTGTGCGCAGACGGCTCACTAACTCTTCTCCAGCGGGAGACGGGCGGGTGACAAGGATACTCATGCCGGGGCGTCTCCGTTATAGACTTCAGCg                              <  2:504243/140‑1 (MQ=255)                                                                                                                  cAATCAGCGGAAAATGCCAGGCCGCCTGCCCCAGTGTGCGCAGACGGCTCACTAACTCTTCTCCAGCGGGAGACGGGCGGGTGACAAGGATACTCATGCCGGGGCGTCTCCGTTATAGACTTCAGCGAGGATCTcgcgcg                 <  2:375095/140‑1 (MQ=255)                                                                                                                  cAATCAGCGGAAAATGCCAGGCCGCCTGCCCCAGTGTGCGCAGACGGCTCACTAACTCTTCTCCAGCGGGAGACGGGCGGGTGACAAGGATACTCATGCCGGGGCGTCTCCGTTATAGACTTCAGCGAGGATCTcgcgcg                 <  1:1064082/140‑1 (MQ=255)                                                                                                                  cAATCAGCGGAAAATGCCAGGCCGCCTGCCCCAGTGTGCGCAGACGGCTCACTAACTCTTCTCCAGCGGGAGACGGGCGGGTGACAAGGATACTCATGCCGGGGCGTCTCCGTTATAGACTTCAGCGAGGATCTcgcgcg                 <  1:1399425/140‑1 (MQ=255)                                                                                                                    aTCAGCGGAAAATGCCAGGCCGCCTGCCCCAGTGTGCGCAGACGGCTCACTAACTCTTCTCCAGCGGGAGACGGGCGGGTGACAAGGATACTCATGCCGGGGCGTCTCCGTTATAGACTTCAGCGAGGATCTcgcgcgcg               <  1:1129107/140‑1 (MQ=255)                                                                                                                     tCAGCGGAAAATGCCAGGCCGCCTGCCCCAGTGTGCGCAGACGGCTCACTAACTCTTCTCCAGCGGGAGACGGGCGGGTGACAAGGATACTCATGCCGGGGCGTCTCCGTTATAGACTTCAGCGAGGATCTCgcgcgcgc              <  1:430524/140‑1 (MQ=255)                                                                                                                       aGCGGAAAATGCCAGGCCGCCTGCCCCAGTGTGCGCAGACGGCTCACTAACTCTTCTCCAGCGGGAGACGGGCGGGTGACAAGGATACTCATGCCGGGGCGTCTCCGTTATAGACTTCAGCGAGGATCTCGCGCGCGCCg            <  2:547097/140‑1 (MQ=255)                                                                                                                       aGCGGAAAATGCCAGGCCGCCTGCCCCAGTGTGCGCAGACGGCTCACTAACTCTTCTCCAGCGGGAGACGGGCGGGTGACAAGGATACTCATGCCGGGGCGTCTCCGTTATAGACTTCAGCGAGGATCTCGCGCGCGCCg            <  2:879709/140‑1 (MQ=255)                                                                                                                       aGCGGAAAATGCCAGGCCGCCTGCCCCAGTGTGCGCAGACGGCTCACTAACTCTTCTCCAGCGGGAGACGGGCGGGTGACAAGGATACTCATGCCGGGGCGTCTCCGTTATAGACTTCAGCGAGGATCTCGCGCGCGCCg            <  1:646364/140‑1 (MQ=255)                                                                                                                             aaaTGCCAGGCCGCCTGCCCCAGTGTGCGCAGACGGCTCACTAACTCTTCTCCAGCGGGAGACGGGCGGGTGACAAGGATACTCATGCCGGGGCGTCTCCGTTATAGACTTCAGCGAGGATCTCGCGCGCGCCGTTATTc      <  2:1012127/140‑1 (MQ=255)                                                                                                                             aaaTGCCAGGCCGCCTGCCCCAGTGTGCGCAGACGGCTCACTAACTCTTCTCCAGCGGGAGACGGGCGGGTGACAAGGATACTCATGCCGGGGCGTCTCCGTTATAGACTTCAGCGAGGATCTCGCGCGCGCCGTTATTc      <  2:284933/140‑1 (MQ=255)                                                                                                                             aaaTGCCAGGCCGCCTGCCCCAGTGTGCGCAGACGGCTCACTAACTCTTCTCCAGCGGGAGACGGGCGGGTGACAAGGATACTCATGCCGGGGCGTCTCCGTTATAGACTTCAGCGAGGATCTCGCGCGCGCCGTTATTc      <  1:920629/140‑1 (MQ=255)                                                                                                                               aTGCCAGGCCGCCTGCCCCAGTGTGCGCAGACGGCTCACTAACTCTTCTCCAGCGGGAGACGGGCGGGTGACAAGGATACTCATGCCGGGGCGTCTCCGTTATAGACTTCAGCGAGGATCTCGCGCGCGCCGTTATTCAg    <  1:101260/140‑1 (MQ=255)                                                                                                                                 gCCAGGCCGCCTGCCCCAGTGTGCGCAGACGGCTCACTAACTCTTCTCCAGCGGGAGACGGGCGGGTGACAAGGATACTCATGCCGGGGCGTCTCCGTTATAGACTTCAGCGAGGATCTCGCGCGCGCCGTTATTCAGTa  <  2:1190976/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                    CTGTGATTGGGCAAAAGCAACCGCGTGTTGCGAGAGGGCAAACAACAGATCGCTCTCCCCCAGCGCTGCCAGTTGATCAGCAAGTTGCGGTAATTGTTGACCCGGAGAAAACTCAATCAGCGGAAAATGCCAGGCCACCTGCCCCAGTGTGCGCAGACGGCTCACTAACTCTTCTCCAGCGGGAGACGGGCGGGTGACAAGGATACTCATGCCGGGGCGTCTCCGTTATAGACTTCAGCGAGGATCTCGCGCGCGCCGTTATTCAGTA  >  NC_000913/3989619‑3989886

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑