BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,994,149	C→T	H100H (CAC→CAT)	yzcX →	protein YzcX

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,994,149	0	C	T	98.0%	350.2 / ‑3.7	102	H100H (CAC→CAT)	yzcX	protein YzcX
Reads supporting (aligned to +/- strand): ref base C (1/1); new base T (51/49); total (52/50)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.41e-01

Only 100 of 102 total aligned reads displayed.

TCAGCCAGAGTTGATCAGCCAGGCGATGAAATTCACTGTCGTTCATTGTTGTATCCTGTTTTTAAGTGATGGCGGCAGTATAGCGGCATGGGGTCAGGGCTTCAAAGTTTGCACCTCTGCGGCTGCGTTCCGGCACGATTCATCCGTCACCGGAATAATGATGTCTCTGTGTAGCGAAAGATTTGTCTCTTCATTAGGGCGCAGTTACACCACGTCTTTCCCTGTTTCTGGTAAACATTATGATCAGGTTTACCGAGCGAGCATCCT  >  NC_000913/3994014‑3994280

tCAGCCAGAGTTGATCAGCCAGGCGATGAAATTCACTGTCGTTCATTGTTGTATCCTGTTTTTAAGTGATGGCGGCAGTATAGCGGCATGGGGTCAGGGCTTCAAAGTTTGCACCTCTGCGGCTGCGTTCCGGCATGAtt                                                                                                                                 >  1:1183001/1‑140 (MQ=255)

tCAGCCAGAGTTGATCAGCCAGGCGATGAAATTCACTGTCGTTCATTGTTGTATCCTGTTTTTAAGTGATGGCGGCAGTATAGCGGCATGGGGTCAGGGCTTCAAAGTTTGCACCTCTGCGGCTGCGTTCCGGCATGAtt                                                                                                                                 >  2:497653/1‑140 (MQ=255)

tCAGCCAGAGTTGATCAGCCAGGCGATGAAATTCACTGTCGTTCATTGTTGTATCCTGTTTTTAAGTGATGGCGGCAGTATAGCGGCATGGGGTCAGGGCTTCAAAGTTTGCACCTCTGCGGCTGCGTTCCGGCATGAtt                                                                                                                                 >  1:1381996/1‑140 (MQ=255)

  aGCCAGAGTTGATCAGCCAGGCGATGAAATTCACTGTCGTTCATTGTTGTATCCTGTTTTTAAGTGATGGCGGCAGTATAGCGGCATGGGGTCAGGGCTTCAAAGTTTGCACCTCTGCGGCTGCGTTCCGGCATGATTCa                                                                                                                               >  2:879824/1‑140 (MQ=255)

       gagTTGATCAGCCAGGCGATGAAATTCACTGTCGTTCATTGTTGTATCCTGTTTTTAAGTGATGGCGGCAGTATAGCGGCATGGGGTCAGGGCTTCAAAGTTTGCACCTCTGCGGCTGCGTTCCGGCATGATTCATCCGt                                                                                                                          >  2:1188321/1‑140 (MQ=255)

         gTTGATCAGCCAGGCGATGAAATTCACTGTCGTTCATTGTTGTATCCTGTTTTTAAGTGATGGCGGCAGTATAGCGGCATGGGGTCAGGGCTTCAAAGTTTGCACCTCTGCGGCTGCGTTCCGGCATGATTCATCCGTCa                                                                                                                        >  1:598278/1‑140 (MQ=255)

         gTTGATCAGCCAGGCGATGAAATTCACTGTCGTTCATTGTTGTATCCTGTTTTTAAGTGATGGCGGCAGTATAGCGGCATGGGGTCAGGGCTTCAAAGTTTGCACCTCTGCGGCTGCGTTCCGGCATGATTCATCCGTCa                                                                                                                        >  1:233215/1‑140 (MQ=255)

          ttGATCAGCCAGGCGATGAAATTCACTGTCGTTCATTGTTGTATCCTGTTTTTAAGTGATGGCGGCAGTATAGCGGCATGGGGTCAGGGCTTCAAAGTTTGCACCTCTGCGGCTGCGTTCCGGCATGATTCATCCGTCAc                                                                                                                       <  1:1072115/140‑1 (MQ=255)

          ttGATCAGCCAGGCGATGAAATTCACTGTCGTTCATTGTTGTATCCTGTTTTTAAGTGATGGCGGCAGTATAGCGGCATGGGGTCAGGGCTTCAAAGTTTGCACCTCTGCGGCTGCGTTCCGGCATGATTCATCCGTCAc                                                                                                                       <  1:646409/140‑1 (MQ=255)

          ttGATCAGCCAGGCGATGAAATTCACTGTCGTTCATTGTTGTATCCTGTTTTTAAGTGATGGCGGCAGTATAGCGGCATGGGGTCAGGGCTTCAAAGTTTGCACCTCTGCGGCTGCGTTCCGGCATGATTCATCCGTCAc                                                                                                                       <  1:150354/140‑1 (MQ=255)

            gATCAGCCAGGCGATGAAATTCACTGTCGTTCATTGTTGTATCCTGTTTTTAAGTGATGGCGGCAGTATAGCGGCATGGGGTCAGGGCTTCAAAGTTTGCACCTCTGCGGCTGCGTTCCGGCATGATTCATCCGTCACCg                                                                                                                     <  1:228303/140‑1 (MQ=255)

             aTCAGCCAGGCGATGAAATTCACTGTCGTTCATTGTTGTATCCTGTTTTTAAGTGATGGCGGCAGTATAGCGGCATGGGGTCAGGGCTTCAAAGTTTGCACCTCTGCGGCTGCGTTCCGGCATGATTCATCCGTCACCgg                                                                                                                    >  1:1026220/1‑140 (MQ=255)

              tCAGCCAGGCGATGAAATTCACTGTCGTTCATTGTTGTATCCTGTTTTTAAGTGATGGCGGCAGTATAGCGGCATGGGGTCAGGGCTTCAAAGTTTGCACCTCTGCGGCTGCGTTCCGGCATGATTCATCCGTCACCGGa                                                                                                                   <  2:1183001/140‑1 (MQ=255)

              tCAGCCAGGCGATGAAATTCACTGTCGTTCATTGTTGTATCCTGTTTTTAAGTGATGGCGGCAGTATAGCGGCATGGGGTCAGGGCTTCAAAGTTTGCACCTCTGCGGCTGCGTTCCGGCATGATTCATCCGTCACCGGa                                                                                                                   <  1:384940/140‑1 (MQ=255)

                  ccAGGCGATGAAATTCACTGTCGTTCATTGTTGTATCCTGTTTTTAAGTGATGGCGGCAGTATAGCGGCATGGGGTCAGGGCTTCAAAGTTTGCACCTCTGCGGCTGCGTTCCGGCATGATTCATCCGTCACCGGaataa                                                                                                               >  2:176795/1‑140 (MQ=255)

                  ccAGGCGATGAAATTCACTGTCGTTCATTGTTGTATCCTGTTTTTAAGTGATGGCGGCAGTATAGCGGCATGGGGTCAGGGCTTCAAAGTTTGCACCTCTGCGGCTGCGTTCCGGCATGATTCATCCGTCACCGGaataa                                                                                                               <  1:1188321/140‑1 (MQ=255)

                  ccAGGCGATGAAATTCACTGTCGTTCATTGTTGTATCCTGTTTTTAAGTGATGGCGGCAGTATAGCGGCATGGGGTCAGGGCTTCAAAGTTTGCACCTCTGAGGCTGCGTTCCGGCACGATTCATCCGTCAc                                                                                                                       >  2:228961/1‑132 (MQ=255)

                  ccAGGCGATGAAATTCACTGTCGTTCATTGTTGTATCCTGTTTTTAAGTGATGGCGGCAGTATAGCGGCATGGGGTCAGGGCTTCAAAGTTTGCACCTCTGAGGCTGCGTTCCGGCACGATTCATCCGTCAc                                                                                                                       <  1:228961/132‑1 (MQ=255)

                    aGGCGATGAAATTCACTGTCGTTCATTGTTGTATCCTGTTTTTAAGTGATGGCGGCTGTATAGCGGCATGGGGTCAGGGCTTCAAAGTTTGCACCTCTGCGGCTGCGTTCCGGCATGATTCATCCGTCACCGGAATAatg                                                                                                             <  1:1243137/140‑1 (MQ=255)

                    aGGCGATGAAATTCACTGTCGTTCATTGTTGTATCCTGTTTTTAAGTGATGGCGGCAGTATAGCGGCATGGGGTCAGGGCTTCAAAGTTTGCACCTCTGCGGCTGCGTTCCGGCATGATTCATCCGTCACCGGAATAatg                                                                                                             >  1:979575/1‑140 (MQ=255)

                    aGGCGATGAAATTCACTGTCGTTCATTGTTGTATCCTGTTTTTAAGTGATGGCGGCAGTATAGCGGCATGGGGTCAGGGCTTCAAAGTTTGCACCTCTGCGGCTGCGTTCCGGCATGATTCATCCGTCACCGGAATAatg                                                                                                             <  2:960229/140‑1 (MQ=255)

                    aGGCGATGAAATTCACTGTCGTTCATTGTTGTATCCTGTTTTTAAGTGATGGCGGCAGTATAGCGGCATGGGGTCAGGGCTTCAAAGTTTGCACCTCTGCGGCTGCGTTCCGGCATGATTCATCCGTCACCGGAATAatg                                                                                                             >  2:539387/1‑140 (MQ=255)

                     ggCGATGAAATTCACTGTCGTTCATTGTTGTATCCTGTTTTTAAGTGATGGCGGCAGTATAGCGGCATGGGGTCAGGGCTTCAAAGTTTGCACCTCTGCGGCTGCGTTCCGGCATGATTCATCCGTCACCGGAATAatga                                                                                                            <  2:656966/140‑1 (MQ=255)

                     ggCGATGAAATTCACTGTCGTTCATTGTTGTATCCTGTTTTTAAGTGATGGCGGCAGTATAGCGGCATGGGGTCAGGGCTTCAAAGTTTGCACCTCTGCGGCTGCGTTCCGGCATGATTCATCCGTCACCGGAATAatga                                                                                                            <  2:1342145/140‑1 (MQ=255)

                     ggCGATGAAATTCACTGTCGTTCATTGTTGTATCCTGTTTTTAAGTGATGGCGGCAGTATAGCGGCATGGGGTCAGGGCTTCAAAGTTTGCACCTCTGCGGCTGCGTTCCGGCATGATTCATCCGTCACCGGAATAatga                                                                                                            <  2:233215/140‑1 (MQ=255)

                     ggCGATGAAATTCACTGTCGTTCATTGTTGTATCCTGTTTTTAAGTGATGGCGGCAGTATAGCGGCATGGGGTCAGGGCTTCAAAGTTTGCACCTCTGCGGCTGCGTTCCGGCATGATTCATCCGTCACCGGAATAatga                                                                                                            <  1:1216932/140‑1 (MQ=255)

                     ggCGATGAAATTCACTGTCGTTCATTGTTGTATCCTGTTTTTAAGTGATGGCGGCAGTATAGCGGCATGGGGTCAGGGCTTCAAAGTTTGCACCTCTGCGGCTGCGTTCCGGCATGATTCATCCGTCACCGGAATAatga                                                                                                            <  1:134538/140‑1 (MQ=255)

                       cGATGAAATTCACTGTCGTTCATTGTTGTATCCTGTTTTTAAGTGATGGCGGCAGTATAGCGGCATGGGGTCAGGGCTTCAAAGTTTGCACCTCTGCGGCTGCGTTCCGGCATGATTCATCCGTCACCGGAATAatgatg                                                                                                          >  1:1030571/1‑140 (MQ=255)

                       cGATGAAATTCACTGTCGTTCATTGTTGTATCCTGTTTTTAAGTGATGGCGGCAGTATAGCGGCATGGGGTCAGGGCTTCAAAGTTTGCACCTCTGCGGCTGCGTTCCGGCATGATTCATCCGTCACCGGAATAatgatg                                                                                                          >  2:1243041/1‑140 (MQ=255)

                          tGAAATTCACTGTCGTTCATTGTTGTATCCTGTTTTTAAGTGATGGCGGCAGTATAGCGGCATGGGGTCAGGGCTTCAAAGTTTGCACCTCTGCGGCTGCGTTCCGGCATGATTCATCCGTCACCGGAATAATGATGtct                                                                                                       <  1:1406757/140‑1 (MQ=255)

                          tGAAATTCACTGTCGTTCATTGTTGTATCCTGTTTTTAAGTGATGGCGGCAGTATAGCGGCATGGGGTCAGGGCTTCAAAGTTTGCACCTCTGCGGCTGCGTTCCGGCATGATTCATCCGTCACCGGAATAATGATGtct                                                                                                       >  1:1330902/1‑140 (MQ=255)

                          tGAAATTCACTGTCGTTCATTGTTGTATCCTGTTTTTAAGTGATGGCGGCAGTATAGCGGCATGGGGTCAGGGCTTCAAAGTTTGCACCTCTGCGGCTGCGTTCCGGCATGATTCATCCGTCACCGGAATAATGATGtct                                                                                                       <  1:539387/140‑1 (MQ=255)

                            aaaTTCACTGTCGTTCATTGTTGTATCCTGTTTTTAAGTGATGGCGGCAGTATAGCGGCATGGGGTCAGGGCTTCAAAGTTTGCACCTCTGCGGCTGCGTTCCGGCATGATTCATCCGTCACCGGAATAATGATGTctct                                                                                                     >  2:511064/1‑140 (MQ=255)

                            aaaTTCACTGTCGTTCATTGTTGTATCCTGTTTTTAAGTGATGGCGGCAGTATAGCGGCATGGGGTCAGGGCTTCAAAGTTTGCACCTCTGCGGCTGCGTTCCGGCATGATTCATCCGTCACCGGAATAATGATGTctct                                                                                                     >  1:1263331/1‑140 (MQ=255)

                               ttCACTGTCGTTCATTGTTGTATCCTGTTTTTAAGTGATGGCGGCAGTATAGCGGCATGGGGTCAGGGCTTCAAAGTTTGCACCTCTGCGGCTGCGTTCCGGCATGATTCATCCGTCACCGGAATAATGATGTCTCtgtg                                                                                                  <  2:798564/140‑1 (MQ=255)

                                    tGTCGTTCATTGTTGTATCCTGTTTTTAAGTGATGGCGGCAGTATAGCGGCATGGGGTCAGGGCTTCAAAGTTTGCACCTCTGCGGCTGCGTCCCGGCATGATTCATCCGTCACCGGAATAATGATGTCTCTGTGTAGCg                                                                                             >  1:1133882/1‑140 (MQ=255)

                                     gTCGTTCATTGTTGTATCCTGTTTTTAAGTGATGGCGGCAGTATAGCGGCATGGGGTCAGGGCTTCAAAGTTTGCACCTCTGCGGCTGCGTTCCGGCATGATTCATCCGTCACCGGAATAATGATGTCTCTGTGTAGCGa                                                                                            <  2:167457/140‑1 (MQ=255)

                                              tgttgtATCCTGTTTTTAAGTGATGGCGGCAGTATAGCGGCATGGGGTCAGGGCTTCAAAGTTTGCACCTCTGCGGCTGCGTTCCGGCATGATTCATCCGTCACCGGAATAATGATGTCTCTGTGTAGCGAAAGATTTGt                                                                                   <  2:1377780/140‑1 (MQ=255)

                                              tgttgtATCCTGTTTTTAAGTGATGGCGGCAGTATAGCGGCATGGGGTCAGGGCTTCAAAGTTTGCACCTCTGCGGCTGCGTTCCGGCATGATTCATCCGTCACCGGAATAATGATGTCTCTGTGTAGCGAAAGATTTGt                                                                                   <  2:1381996/140‑1 (MQ=255)

                                                ttgtATCCTGTTTTTAAGTGATGGCGGCAGTATAGCGGCATGGGGTCAGGGCTTCAAAGTTTGCACCTCTGCGGCTGCGTTCCGGCATGATTCATCCGTCACCGGAATAATGATGTCTCTGTGTAGCGAAAGATTTGtct                                                                                 <  2:581254/140‑1 (MQ=255)

                                                ttgtATCCTGTTTTTAAGTGATGGCGGCAGTATAGCGGCATGGGGTCAGGGCTTCAAAGTTTGCACCTCTGCGGCTGCGTTCCGGCATGATTCATCCGTCACCGGAATAATGATGTCTCTGTGTAGCGAAAGATTTGtct                                                                                 <  2:555528/140‑1 (MQ=255)

                                                ttgtATCCTGTTTTTAAGTGATGGCGGCAGTATAGCGGCATGGGGTCAGGGCTTCAAAGTTTGCACCTCTGCGGCTGCGTTCCGGCATGATTCATCCGTCACCGGAATAATGATGTCTCTGTGTAGCGAAAGATTTGtct                                                                                 >  1:371584/1‑140 (MQ=255)

                                                 tgtATCCTGTTTTTAAGTGATGGCGGCAGTATAGCGGCATGGGGTCAGGGCTTCAAAGTTTGCACCTCTGCGGCTGCGTTCCGGCATGATTCATCCGTCACCGGAATAATGATGTCTCTGTGTAGCGAAAGATTTGtctc                                                                                <  1:548444/140‑1 (MQ=255)

                                                  gtATCCTGTTTTTAAGTGATGGCGGCAGTATAGCGGCATGGGGTCAGGGCTTCAAAGTTTGCACCTCTGCGGCTGCGTTCCGGCATGATTCATCCGTCACCGGAATAATGATGTCTCTGTGTAGCGAAAGATTTGTctct                                                                               >  2:1294829/1‑140 (MQ=255)

                                                     tCCTGTTTTTAAGTGATGGCGGCAGTATAGCGGCATGGGGTCAGGGCTTCAAAGTTTGCACCTCTGCGGCTGCGTTCCGGCATGATTCATCCGTCACCGGAATAATGATGTCTCTGTGTAGCGAAAGATTTGTCTCTTCa                                                                            >  1:570864/1‑140 (MQ=255)

                                                     tCCTGTTTTTAAGTGATGGCGGCAGTATAGCGGCATGGGGTCAGGGCTTCAAAGTTTGCACCTCTGCGGCTGCGTTCCGGCATGATTCATCCGTCACCGGAATAATGATGTCTCTGTGTAGCGAAAGATTTGTCTCTTCa                                                                            >  1:1030632/1‑140 (MQ=255)

                                                       cTGTTTTTAAGTGATGGCGGCAGTATAGCGGCATGGGGTCAGGGCTTCAAAGTTTGCACCTCTGCGGCTGCGTTCCGGCATGATTCATCCGTCACCGGAATAATGATGTCTCTGTGTAGCGAAAGATTTGTCTCTTCAtt                                                                          <  2:1330902/140‑1 (MQ=255)

                                                          tttttAAGTGATGGCGGCAGTATAGCGGCATGGGGTCAGGGCTTCAAAGTTTGCACCTCTGGGGCTGCGTTCCGGCATGATTCATCCGTCACCGGAATAATGATGTCTCTGTGTAGCGAAAGATTTGTCTCTTCATTAgg                                                                       >  2:198161/1‑140 (MQ=255)

                                                          tttttAAGTGATGGCGGCAGTATAGCGGCATGGGGTCAGGGCTTCAAAGTTTGCACCTCTGCGGCTGCGTTCCGGCATGATTCATCCGTCACCGGAATAATGATGTCTCTGTGTAGCGAAAGATTTGTCTCTTCATTAgg                                                                       >  1:209470/1‑140 (MQ=255)

                                                          tttttAAGTGATGGCGGCAGTATAGCGGCATGGGGTCAGGGCTTCAAAGTTTGCACCTCTGCGGCTGCGTTCCGGCATGATTCATCCGTCACCGGAATAATGATGTCTCTGTGTAGCGAAAGATTTGTCTCTTCATTAgg                                                                       >  2:957415/1‑140 (MQ=255)

                                                          tttttAAGTGATGGCGGCAGTATAGCGGCATGGGGTCAGGGCTTCAAAGTTTGCACCTCTGCGGCTGCGTTCCGGCATGATTCATCCGTCACCGGAATAATGATGTCTCTGTGTAGCGAAAGATTTGTCTCTTCATTAgg                                                                       >  1:469624/1‑140 (MQ=255)

                                                          tttttAAGTGATGGCGGCAGTATAGCGGCATGGGGTCAGGGCTTCAAAGTTTGCACCTCTGCGGCTGCGTTCCGGCATGATTCATCCGTCACCGGAATAATGATGTCTCTGTGTAGCGAAAGATTTGTCTCTTCATTAgg                                                                       >  1:1145725/1‑140 (MQ=255)

                                                           ttttAAGTGATGGCGGCAGTATAGCGGCATGGGGTCAGGGCTTCAAAGTTTGCACCTCTGCGGCTGCGTTCCGGCATGATTCATCCGTCACCGGAATAATGATGTCTCTGTGTAGCGAAAGATTTGTCTCTTCATTAggg                                                                      <  2:371584/140‑1 (MQ=255)

                                                            tttAAGTGATGGCGGCAGTATAGCGGCATGGGGTCAGGGCTTCAAAGTTTGCACCTCTGCGGCTGCGTTCCGGCATGATTCATCCGTCACCGGAATAATGATGTCTCTGTGTAGCGAAAGATTTGTCTCTTCATTAGGgc                                                                     <  1:957415/140‑1 (MQ=255)

                                                            tttAAGTGATGGCGGCAGTATAGCGGCATGGGGTCAGGGCTTCAAAGTTTGCACCTCTGCGGCTGCGTTCCGGCATGATTCATCCGTCACCGGAATAATGATGTCTCTGTGTAGCGAAAGATTTGTCTCTTCATTAGGgc                                                                     <  1:176795/140‑1 (MQ=255)

                                                              tAAGTGATGGCGGCAGTATAGCGGCATGGGGTCAGGGCTTCAAAGTTTGCACCTCTGCGGCTGCGTTCCGGCATGATTCATCCGTCACCGGAATAATGATGTCTCTGTGTAGCGAAAGATTTGTCTCTTCATTAGGgcgc                                                                   <  2:1263331/140‑1 (MQ=255)

                                                                           cAGTATAGCGGCATGGGGTCAGGGCTTCAAAGTTTGCACCTCTGCGGCTGCGTTCCGGCATGATTCATCCGTCACCGGAATAATGATGTCTCTGTGTAGCGAAAGATTTGTCTCTTCATTAGGGCGCAGTTACACCACGt                                                      >  2:1392433/1‑140 (MQ=255)

                                                                             gTATAGCGGCATGGGGTCAGGGCTTCAAAGTTTGCACCTCTGCGGCTGCGTTCCGGCATGATTCATCCGTCACCGGAATAATGATGTCTCTGTGTAGCGAAAGATTTGTCTCTTCATTAGGGCGCAGTTACACCACGTCt                                                    <  1:198161/140‑1 (MQ=255)

                                                                                taGCGGCATGGGGTCAGGGCTTCAAAGTTTGCACCTCTGCGGCTGCGTTCCGGCATGATTCATCCGTCACCGGAATAATGATGTCTCTGTGTAGCGAAAGATTTGTCTCTTCATTAGGGCGCAGTTACACCACGTCTTTc                                                 >  2:468766/1‑140 (MQ=255)

                                                                                taGCGGCATGGGGTCAGGGCTTCAAAGTTTGCACCTCTGCGGCTGCGTTCCGGCATGATTCATCCGTCACCGGAATAATGATGTCTCTGTGTAGCGAAAGATTTGTCTCTTCATTAGGGCGCAGTTACACCACGTCTTTc                                                 >  2:897632/1‑140 (MQ=255)

                                                                                taGCGGCATGGGGTCAGGGCTTCAAAGTTTGCACCTCTGCGGCTGCGTTCCGGCATGATTCATCCGTCACCGGAATAATGATGTCTCTGTGTAGCGAAAGATTTGTCTCTTCATTAGGGCGCAGTTACACCACGTCTTTc                                                 >  2:930897/1‑140 (MQ=255)

                                                                                     gCATGGGGTCAGGGCTTCAAAGTTTGCACCTCTGCGGCTGCGTTCCGGCATGATTCATCCGTCACCGGAATAATGATGTCTCTGTGTAGCGAAAGATTTGTCTCTTCATTAGGGCGCAGTTACACCACGTCTTTCCCTGt                                            <  1:1306251/140‑1 (MQ=255)

                                                                                      cATGGGGTCAGGGCTTCAAAGTTTGCACCTCTGCGGCTGCGTTCCGGCATGATTCATCCGTCACCGGAATAATGATGTCTCTGTGTAGCGAAAGATTTGTCTCTTCATTAGGGCGCAGTTACACCACGTCTTTCCCTGtt                                           <  2:598278/140‑1 (MQ=255)

                                                                                        tGGGGTCAGGGCTTCAAAGTTTGCACCTCTGCGGCTGCGTTCCGGCATGATTCATCCGTCACCGGAATAATGATGTCTCTGTGTAGCGAAAGATTTGTCTCTTCATTAGGGCGCAGTTAcacc                                                          <  1:24453/123‑1 (MQ=255)

                                                                                        tGGGGTCAGGGCTTCAAAGTTTGCACCTCTGCGGCTGCGTTCCGGCATGATTCATCCGTCACCGGAATAATGATGTCTCTGTGTAGCGAAAGATTTGTCTCTTCATTAGGGCGCAGTTAcacc                                                          >  2:24453/1‑123 (MQ=255)

                                                                                        tGGGGTCAGGGCTTCAAAGTTTGCACCTCTGCGGCTGCGTTCCGGCATGATTCATCCGTCACCGGAATAATGATGTCTCTGTGTAGCGAAAGATTTGTCTCTTCATTAGGGCGCAGTTACACCACGTCTTTCCCTGTTTc                                         >  2:117655/1‑140 (MQ=255)

                                                                                        tGGGGTCAGGGCTTCAAAGTTTGCACCTCTGCGGCTGCGTTCCGGCATGATTCATCCGTCACCGGAATAATGATGTCTCTGTGTAGCGAAAGATTTGTCTCTTCATTAGGGCGCAGTTACACCACGTCTTTCCCTGTTTc                                         >  1:286870/1‑140 (MQ=255)

                                                                                        tGGGGTCAGGGCTTCAAAGTTTGCACCTCTGCGGCTGCGTTCCGGCATGATTCATCCGTCACCGGAATAATGATGTCTCTGTGTAGCGAAAGATTTGTCTCTTCATTAGGGCGCAGTTACACCACGTCTTTCCCTGTTTc                                         >  1:733181/1‑140 (MQ=255)

                                                                                        tGGGGTCAGGGCTTCAAAGTTTGCACCTCTGCGGCTGCGTTCCGGCATGATTCATCCGTCACCGGAATAATGATGTCTCTGTGTAGCGAAAGATTTGTCTCTTCATTAGGGCGCAGTTACACCACGTCTTTCCCTGTTTc                                         >  2:1394277/1‑140 (MQ=255)

                                                                                            gTCAGGGCTTCAAAGTTTGCACCTCTGCGGCTGCGTTCCGGCATGATTCATCCGTCACCGGAATAATGATGTCTCTGTGTAGCGAAAGATTTGTCTCTTCATTAGGGCGCAGTTACACCACGTCTTTCCCTGTTTCTGGt                                     <  1:1392433/140‑1 (MQ=255)

                                                                                            gTCAGGGCTTCAAAGTTTGCACCTCTGCGGCTGCGTTCCGGCATGATTCATCCGTCACCGGAATAATGATGTCTCTGTGTAGCGAAAGATTTGTCTCTTCATTAGGGCGCAGTTACACCACGTCTTTCCCTGTTTCTGGt                                     <  1:879824/140‑1 (MQ=255)

                                                                                             tCAGGGCTTCAAAGTTTGCACCTCTGCGGCTGCGTTCCGGCATGATTCATCCGTCACCGGAATAATGATGTCTCTGTGTAGCGAAAGATTTGTCTCTTCATTAGGGCGCAGTTACACCACGTCTTTCCCTGTTTCTGGTa                                    <  1:511064/140‑1 (MQ=255)

                                                                                             tCAGGGCTTCAAAGTTTGCACCTCTGCGGCTGCGTTCCGGCATGATTCATCCGTCACCGGAATAATGATGTCTCTGTGTAGCGAAAGATTTGTCTCTTCATTAGGGCGCAGTTACACCACGTCTTTCCCTGTTTCTGGTa                                    <  2:469624/140‑1 (MQ=255)

                                                                                              cAGGGCTTCAAAGTTTGCACCTCTGCGGCTGCGTTCCGGCATGATTCATCCGTCACCGGAATAATGATGTCTCTGTGTAGCGAAAGATTTGTCTCTTCATTAGGGCGCAGTTACACCACGTCTTTCCCTGTTTCTGGTaa                                   <  1:141908/140‑1 (MQ=255)

                                                                                              cAGGGCTTCAAAGTTTGCACCTCTGCGGCTGCGTTCCGGCATGATTCATCCGTCACCGGAATAATGATGTCTCTGTGTAGCGAAAGATTTGTCTCTTCATTAGGGCGCAGTTACACCACGTCTTTCCCTGTTTCTGGTaa                                   <  2:320114/140‑1 (MQ=255)

                                                                                               aGGGCTTCAAAGTTTGCACCTCTGCGGCTGCGTTCCGGTATGATTCATCCGTCACCGGAATAATGATGTCTCTGTGTAGCGAAAGATTTGTCTCTTCATTAGGGCGCAGTTACACCACGTCTTTCCCTGTTTCTGGTaaa                                  >  2:41391/1‑140 (MQ=255)

                                                                                                gggCTTCAAAGTTTGCACCTCTGCGGCTGCGTTCCGGCATGATTCATCCGTCACCGGAATAATGATGTCTCTGTGTAGCGAAAGATTTGTCTCTTCATTAGGGCGCAGTTACACCACGTCTTTCCCTGCTTCTGGTAAAc                                 >  2:1217534/1‑140 (MQ=255)

                                                                                                     tCAAAGTTTGCACCTCTGCGGCTGCGTTCCGGCATGATTCATCCGTCACCGGAATAATGATGTCTCTGTGTAGCGAAAGATTTGTCTCTTCATTAGGGCGCAGTTACACCACGTCTTTCCCTGTTTCTGGt                                     <  1:732937/131‑1 (MQ=255)

                                                                                                     tCAAAGTTTGCACCTCTGCGGCTGCGTTCCGGCATGATTCATCCGTCACCGGAATAATGATGTCTCTGTGTAGCGAAAGATTTGTCTCTTCATTAGGGCGCAGTTACACCACGTCTTTCCCTGTTTCTGGt                                     >  2:732937/1‑131 (MQ=255)

                                                                                                     tCAAAGTTTGCACCTCTGCGGCTGCGTTCCGGCATGATTCATCCGTCACCGGAATAATGATGTCTCTGTGTAGCGAAAGATTTGTCTCTTCATTAGGGCGCAGTTACACCACGTCTTTCCCTGTTTCTGGTAAACATTAt                            >  1:751274/1‑140 (MQ=255)

                                                                                                      cAAAGTTTGCACCTCTGCGGCTGCGTTCCGGCATGATTCATCCGTCACCGGAATAATGATGTCTCTGTGTAGCGAAAGATTTGTCTCTTCATTAGGGCGCAGTTACACCACGTCTTTCCCTGTTTCTGGTAAACATTATg                           >  1:140069/1‑140 (MQ=255)

                                                                                                      cAAAGTTTGCACCTCTGCGGCTGCGTTCCGGCATGATTCATCCGTCACCGGAATAATGATGTCTCTGTGTAGCGAAAGATTTGTCTCTTCATTAGGGCGCAGTTACACCACGTCTTTCCCTGTTTCTGGTAAACATTATg                           >  1:1111204/1‑140 (MQ=255)

                                                                                                      cAAAGTTTGCACCTCTGCGGCTGCGTTCCGGCATGATTCATCCGTCACCGGAATAATGATGTCTCTGTGTAGCGAAAGATTTGTCTCTTCATTAGGGCGCAGTTACACCACGTCTTTCCCTGTTTCTGGTAAACATTATg                           <  2:346450/140‑1 (MQ=255)

                                                                                                      cAAAGTTTGCACCTCTGCGGCTGCGTTCCGGCATGATTCATCCGTCACCGGAATAATGATGTCTCTGTGTAGCGAAAGATTTGTCTCTTCATTAGGGCGCAGTTACACCACGTCTTTCCCTGTTTCTGGTAAACATTATg                           >  1:1150947/1‑140 (MQ=255)

                                                                                                        aaGTTTGCACCTCTGCGGCTGCGTTCCGGCATGATTCATCCGTCACCGGAATAATGATGTCTCTGTGTAGCGAAAGATTTGTCTCTTCATTAGGGCGCAGTTACACCACGTCTTTCCCTGTTTCTGGTAAACATTATGAt                         <  2:1111204/140‑1 (MQ=255)

                                                                                                        aaGTTTGCACCTCTGCGGCTGCGTTCCGGCATGATTCATCCGTCACCGGAATAATGATGTCTCTGTGTAGCGAAAGATTTGTCTCTTCATTAGGGCGCAGTTACACCACGTCTTTCCCTGTTTCTGGTAAACATTATGAt                         <  2:70766/140‑1 (MQ=255)

                                                                                                        aaGTTTGCACCTCTGCGGCTGCGTTCCGGCATGATTCATCCGTCACCGGAATAATGATGTCTCTGTGTAGCGAAAGATTTGTCTCTTCATTAGGGCGCAGTTACACCACGTCTTTCCCTGTTTCTGGTAAACATTATGAt                         <  2:1030571/140‑1 (MQ=255)

                                                                                                        aaGTTTGCACCTCTGCGGCTGCGTTCCGGCATGATTCATCCGTCACCGGAATAATGATGTCTCTGTGTAGCGAAAGATTTGTCTCTTCATTAGGGCGCAGTTACACCACGTCTTTCCCTGTTTCTGGTAAACATTATGAt                         <  2:1030632/140‑1 (MQ=255)

                                                                                                           tttGCACCTCTGCGGCTGCGTTCCGGCATGATTCATCCGTCACCGGAATAATGATGTCTCTGTGTAGCGAAAGATTTGTCTCTTCATTAGGGCGCAGTTACACCACGTCTTTCCCTGTTTCTGGTAAACATTATGATCAg                      >  1:601961/1‑140 (MQ=255)

                                                                                                           tttGCACCTCTGCGGCTGCGTTCCGGCATGATTCATCCGTCACCGGAATAATGATGTCTCTGTGTAGCGAAAGATTTGTCTCTTCATTAGGGCGCAGTTACACCACGTCTTTCCCTGTTTCTGGTAAACATTATGATCAg                      >  1:1124587/1‑140 (MQ=255)

                                                                                                           tttGCACCTCTGCGGCTGCGTTCCGGCATGATTCATCCGTCACCGGAATAATGATGTCTCTGTGTAGCGAAAGATTTGTCTCTTCATTAGGGCGCAGTTACACCACGTCTTTCCCTGTTTCTGGTAAACATTATGATCAg                      >  1:869746/1‑140 (MQ=255)

                                                                                                           tttGCACCTCTGCGGCTGCGTTCCGGCATGATTCATCCGTCACCGGAATAATGATGTCTCTGTGTAGCGAAAGATTTGTCTCTTCATTAGGGCGCAGTTACACCACGTCTTTCCCTGTTTCTGGTAAACATTATGATCAg                      >  2:96925/1‑140 (MQ=255)

                                                                                                                    ctGCGGCTGCGTTCCGGCATGATTCATCCGTCACCGGAATAATGATGTCTCTGTGTAGCGAAAGATTTGTCTCTTCATTAGGGCGCAGTTACACCACGTCTTTCCCTGTTTCTGGTAAACATTATGATCAGGTTTACcga             <  1:1243041/140‑1 (MQ=255)

                                                                                                                         gCTGCGTTCCGGTATGATTCATCCGTCACCGGAATAATGATGTCTCTGTGTAGCGAAAGATTTGTCTCTTCATTAGGGCGCAGTTACACCACGTCTTTCCCTGTTTCTGGTAAACATTATGATCAGGTTTACcgagcgag        <  1:41391/140‑1 (MQ=255)

                                                                                                                         gCTGCGTTCCGGCATGATTCATCCGTCACCGGAATAATGATGTCTCTGTGTAGCGAAAGATTTGTCTCTTCATTAGGGCGCAGTTACACCACGTCTTTCCCTGTTTCTGGTAAACATTATGATCAGGTTTACcgagcgag        <  2:1124587/140‑1 (MQ=255)

                                                                                                                          cTGCGTTCCGGCATGATTCATCCGTCACCGGAATAATGATGTCTCTGTGTAGCGAAAGATTTGTCTCTTCATTAGGGCGCAGTTACACCACGTCTTTCCCTGTTTCTGGTAAACATTATGATCAGGTTTACCgagcgagc       <  2:140069/140‑1 (MQ=255)

                                                                                                                            gCGTTCCGGCATGATTCATCCGTCACCGGAATAATGATGTCTCTGTGTAGCGAAAGATTTGTCTCTTCATTAGGGCGCAGTTACACCACGTCTTTCCCTGTTTCTGGTAAACATTATGATCAGGTTTACCGAGCGAGCAt     >  2:296379/1‑140 (MQ=255)

                                                                                                                             cGTTCCGGCATGATTCATCCGTCACCGGAATAATGATGTCTCTGTGTAGCGAAAGATTTGTCTCTTCATTAGGGCGCAGTTACACCACGTCTTTCCCTGTTTCTGGTAAACATTATGATCAGGTTTACCGAGCGAGCATc    >  1:528100/1‑140 (MQ=255)

                                                                                                                               ttCCGGCATGATTCATCCGTCACCGGAATAATGATGTCTCTGTGTAGCGAAAGATTTGTCTCTTCATTAGGGCGCAGTTACACCACGTCTTTCCCTGTTTCTGGTAAACATTATGATCAGGTTTACCGAGCGAGCATCCt  >  1:830755/1‑140 (MQ=255)

                                                                                                                               ttCCGGCATGATTCATCCGTCACCGGAATAATGATGTCTCTGTGTAGCGAAAGATTTGTCTCTTCATTAGGGCGCAGTTACACCACGTCTTTCCCTGTTTCTGGTAAACATTATGATCAGGTTTACCGAGCGAGCATCCt  >  2:1178846/1‑140 (MQ=255)

TCAGCCAGAGTTGATCAGCCAGGCGATGAAATTCACTGTCGTTCATTGTTGTATCCTGTTTTTAAGTGATGGCGGCAGTATAGCGGCATGGGGTCAGGGCTTCAAAGTTTGCACCTCTGCGGCTGCGTTCCGGCACGATTCATCCGTCACCGGAATAATGATGTCTCTGTGTAGCGAAAGATTTGTCTCTTCATTAGGGCGCAGTTACACCACGTCTTTCCCTGTTTCTGGTAAACATTATGATCAGGTTTACCGAGCGAGCATCCT  >  NC_000913/3994014‑3994280

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`