Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,083,554	C→T	A757T (GCC→ACC)	fdoG ←	formate dehydrogenase O subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,083,554	0	C	T	100.0%	166.3 / NA	51	A757T (GCC→ACC)	fdoG	formate dehydrogenase O subunit alpha
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (24/27);  total (24/27)

TCCCATTTCAGCAACTGACGCTTCGGATCCCACGGGTTACCCTGCGGATCTGCGGAGGCGCGGTTATACAGAATGCGGCGGTTAAGCGGCCATGCCCATGCCCAGCCCAGCGTGTTACCGAGGCCAGACGGATCGGCGTTATCACGGCGTGCCATCTGGTTGCCTTCCGGCGTCCAGCTACCGGCGAAAATCCAGCAGCCACAGGAGGTTGTACCGTCATCGCGCAATTGGGCGAACGAACTAAGTTGTTGGCCTTTCTTGACGATAACCGCA  >  NC_000913/4083418‑4083690                                                                                                                                         |                                                                                                                                         tCCCATTTCAGCAACTGACGCTTCGGATCCCACGGGTTACCCTGCGGATCTGCGGAGGCGCGGTTATACAGAATGCGGCGGTTAAGCGGCCATGCCCATGCCCAGCCCAGCGTGTTACCGAGGCCAGACGGATCGGTGtt                                                                                                                                       >  1:217352/1‑140 (MQ=255)      tttCAGCAACTGACGCTTCGGATCCCACGGGTTACCCTGCGGATCTGCGGAGGCGCGGTTATACAGAATGCGGCGGTTAAGCGGCCATGCCCATGCCCAGCCCAGCGTGTTACCGAGGCCAGACGGATCGGTGTTATCAc                                                                                                                                  <  2:1025390/140‑1 (MQ=255)      tttCAGCAACTGACGCTTCGGATCCCACGGGTTACCCTGCGGATCTGCGGAGGCGCGGTTATACAGAATGCGGCGGTTAAGCGGCCATGCCCATGCCCAGCCCAGCGTGTTACCGAGGCCAGACGGATCGGTGTTATCAc                                                                                                                                  <  1:1075751/140‑1 (MQ=255)       ttCAGCAACTGACGCTTCGGATCCCACGGGTTACCCTGCGGATCTGCGGAGGCGCGGTTATACAGAATGCGGCGGTTAAGCGGCCATGCCCATGCCCAGCCCAGCGTGTTACCGAGGCCAGACGGATCGGTGTTATCACg                                                                                                                                 <  1:765450/140‑1 (MQ=255)          aGCAACTGACGCTTCGGATCCCACGGGTTACCCTGCGGATCTGCGGAGGCGCGGTTATACAGAATGCGGCGGTTAAGCGGCCATGCCCATGCCCAGCCCAGCGTGTTACCGAGGCCAGACGGATCGGTGTTATCACGGCg                                                                                                                              <  1:237836/140‑1 (MQ=255)               cTGACGCTTCGGATCCCACGGGTTACCCTGCGGATCTGCGGAGGCGCGGTTATACAGAATGCGGCGGTTAAGCGGCCATGCCCATGCCCAGCCCAGCGTGTTACCGAGGCCAGACGGATCGGTGTTATCACGGCGTGCCa                                                                                                                         <  1:168169/140‑1 (MQ=255)                      ttCGGATCCCACGGGTTACCCTGCGGATCTGCGGAGGCGCGGTTATACAGAATGCGGCGGTTAAGCGGCCATGCCCATGCCCAGCCCAGCGTGTTACCGAGGCCAGACGGATCGGTGTTATCACGGCGTGCCATCTGGtt                                                                                                                  >  1:166304/1‑140 (MQ=255)                       tCGGATCCCACGGGTTACCCTGCGGATCTGCGGAGGCGCGGTTATACAGAATGCGGCGGTTAAGCGGCCATGCCCATGCCCAGCCCAGCGTGTTACCGAGGCCAGACGGATCGGTGTTATCACGGCGTGCCATCTGGTTg                                                                                                                 >  2:1176485/1‑140 (MQ=255)                                aCGGGTTACCCTGCGGATCTGCGGAGGCGCGGTTATACAGAATGCGGCGGTTAAGCGGCCATGCCCATGCCCAGCCCAGCGTGTTACCGAGGCCAGACGGATCGGTGTTATCACGGCGTGCCATCTGGTTGCCTTCCGGc                                                                                                        >  2:70417/1‑140 (MQ=255)                                     ttACCCTGCGGATCTGCGGAGGCGCGGTTATACAGAATGCGGCGGTTAAACGGCCCTGCCCATGCCCAGCCCAGCGGGTTAACGAGGCCAGACGGATCGGGGTTATCACGGCGTGCCCTCTGGTTGCCTTCCCGCGTccc                                                                                                   >  2:1003956/1‑139 (MQ=255)                                       aCCCTGCGGATCTGCGGAGGCGCGGTTATACAGAATGCGGCGGTTAAGCGGCCATGCCCATGCCCCGCCCAGCGTGTTACCGAGGCCAGACGGATCGGGGTTATCACGGCGTGCCATCTGGGTGCCTTCCCGCGTCCCGc                                                                                                 >  2:1227848/1‑140 (MQ=255)                                          cTGCGGATCTGCGGAGGCGCGGTTATACAGAATGCGGCGGTTAAGCGGCCATGCCCATGCCCAGCCCAGCGTGTTACCGAGGCCAGACGGATCGGTGTTATCACGGCGTGCCATCTGGTTGCCTTCCGGCGTCCAGCTAc                                                                                              <  2:603712/140‑1 (MQ=255)                                             cGGATCTGCGGAGGCGCGGTTATACAGAATGCGGCGGTTAAGCGGCCATGCCCATGCCCAGCCCAGCGTGTTACCGAGGCCAGACGGATCGGTGTTATCACGGCGTGCCATCTGGTTGCCTTc                                                                                                            <  2:521880/123‑1 (MQ=255)                                             cGGATCTGCGGAGGCGCGGTTATACAGAATGCGGCGGTTAAGCGGCCATGCCCATGCCCAGCCCAGCGTGTTACCGAGGCCAGACGGATCGGTGTTATCACGGCGTGCCATCTGGTTGCCTTc                                                                                                            >  1:521880/1‑123 (MQ=255)                                             cGGATCTGCGGAGGCGCGGTTATACAGAATGCGGCGGTTAAACGGCCCTGCCCCTGCCCCGCCCAGCGGGTTACCGAGGCCAGACGGATCGGGGTTATCACCGCGTGCCATCTGGTTGCCTTCCGGCGGCCAGCCACCgg                                                                                           >  2:100602/1‑140 (MQ=255)                                                aTCTGCGGAGGCGCGGTTATACAGAATGCGGCGGTTAAGCGGCCATGCCCATGCCCAGCCCAGCGTGTTACCGAGGCCAGACGGGTCGGGGTTATCACGGCGTGCCATCTGGTTGCCTTTCCGCGTCCCGCTACCGGCGa                                                                                        >  2:287212/1‑140 (MQ=255)                                                   tGCGGAGGCGCGGTTATACAGAATGCGGCGGTTAAGCGGCCCTGCCCATGCCCAGCCCCGCGTGTTACCGAGGCCCGACGGGTCGGGGTTATTACGGCGTGCCATCTGGTTGCCTTCCGGCGTCCAGCTACCCGCGaaaa                                                                                     >  2:827914/1‑140 (MQ=255)                                                       gAGGCGCGGTTATACAGAATGCGGCGGTTAAGCGGCCATGCCCATGCCCAGCCCAGCGTGTTACCGAGGCCAGACGGATCGGTGTTATCACGGCGTGCCATCTGGTTGCCTTCCGGCGTCCAGCTACCGGCGAAAATCca                                                                                 <  1:1176485/140‑1 (MQ=255)                                                       gAGGCGCGGTTATACAGAATGCGGCGGTTAAGCGGCCATGCCCATGCCCAGCCCAGCGTGTTACCGAGGCCAGACGGATCGGTGTTATCACGGCGTGCCATCTGGTTGCCTTCCGGCGTCCAGCTACCGGCGAAAATCca                                                                                 <  1:1409568/140‑1 (MQ=255)                                                       gAGGCGCGGTTATACAGAATGCGGCGGTTAAGCGGCCATGCCCATGCCCAGCCCAGCGTGTTACCGAGGCCAGACGGATCGGTGTTATCACGGCGTGCCATCTGGTTGCCTTCCGGCGTCCAGCTACCGGCGAAAATCca                                                                                 <  2:266378/140‑1 (MQ=255)                                                        aGGCGCGGTTATACAGAATGCGGCGGTTAAGCGGCCATGCCCATGCCCAGCCCAGCGTGTTACCGAGGCCAGACGGATCGGTGTTATCACGGCGTGCCATCTGGTTGCCTTCCGGCGTCCAGCTACCGGCGAAAATCcag                                                                                <  1:1003956/140‑1 (MQ=255)                                                          gcgcGGTTATACAGAATGCGGCGGTTAAGCGGCCATGCCCATGCCCAGCCCAGCGTGTTACCGAGGCCAGACGGATCGGTGTTATCACGGCGTGCCATCTGGTTGCCTTCCGGCGTCCAGCTACCGGCGAAAATCcagca                                                                              >  1:453025/1‑140 (MQ=255)                                                              ggTTATACAGAATGCGGCGGTTAAGCGGCCATGCCCATGCCCAGCCCAGCGTGTTACCGAGGCCAGACGGATCGGTGTTATCACGGCGTGCCATCTGGTTGCCTTCCGGCGTCCAGCTACCGGCGAAAATCCAGCAGCca                                                                          <  1:70417/140‑1 (MQ=255)                                                                ttATACAGAATGCGGCGGTTAAGCGGCCATGCCCATGCCCAGCCCAGCGTGTTACCGAGGCCAGACGGATCGGTGTTATCACGGCGTGCCATCTGGTTGCCTTCCGGCGTCCAGCTACCGGCGAAAATCCAGCAGCcaca                                                                        <  1:983668/140‑1 (MQ=255)                                                                ttATACAGAATGCGGCGGTTAAGCGGCCATGCCCATGCCCAGCCCAGCGTGTTACCGAGGCCAGACGGATCGGTGTTATCACGGCGTGCCATCTGGTTGCCTTCCGGCGTCCAGCTACCGGCGAAAATCCAGCAGCcaca                                                                        <  2:217352/140‑1 (MQ=255)                                                                 tataCAGAATGCGGCGGTTAAGCGGCCATGCCCATGCCCAGTCCAGCGTGTTACCGAGGCCAGACGGATCGGTGTTATCACGGCGTGCCATCTGGTTGCCTTCCGGCGTCCAGCTACCGGCGAAAATCCAGCAGCCACag                                                                       <  1:463564/140‑1 (MQ=255)                                                                  ataCAGAATGCGGCGGTTAAGCGGCCATGCCCATGCCCAGCCCAGCGTGTTACCGAGGCCAGACGGATCGGTGTTATCACGGCGTGCCATCTGGTTGCCTTCCGGCGTCCAGCTACCGGCGAAAATCCAGCAGCCACagg                                                                      <  2:453025/140‑1 (MQ=255)                                                                  ataCAGAATGCGGCGGTTAAGCGGCCATGCCCATGCCCAGCCCAGCGTGTTACCGAGGCCAGACGGATCGGTGTTATCACGGCGTGCCATCTGGTTGCCTTCCGGCGTCCAGCTACCGGCGAAAATCCAGCAGCCACagg                                                                      <  1:448632/140‑1 (MQ=255)                                                                  ataCAGAATGCGGCGGTTAAGCGGCCATGCCCATGCCCAGCCCAGCGTGTTACCGAGGCCAGACGGATCGGTGTTATCACGGCGTGCCATCTGGTTGCCTTCCGGCGCCCAGCTACCGGCGAAAATCCAGCAGCCACagg                                                                      <  2:1218156/140‑1 (MQ=255)                                                                   taCAGAATGCGGCGGTTAAGCGGCCATGCCCATGCCCAGCCCAGCGTGTTACCGAGGCCAGACGGATCGGTGTTATCACGGCGTGCCATCTGGTTGCCTTCCGGCGTCCAGCTACCGGCGAAAATCCAGCAGCCACagga                                                                     <  1:2345/140‑1 (MQ=255)                                                                        aaTGCGGCGGTTAAGCGGCCATGCCCATGCCCAGCCCAGCGTGTTACCGAGGCCAGACGGATCGGTGTTATCACGGCGTGCCATCTGGTTGCCTTCCGGCGTCCAGCTACCGGCGAAAATCCAGCAGCCACAGGAGGTTg                                                                >  2:484809/1‑140 (MQ=255)                                                                          tGCGGCGGTTAAGCGGCCATGCCCATGCCCAGCCCAGCGTGTTACCGAGGCCAGACGGATCGGTGTTATCACGGCGTGCCATCTGGTTGCCTTCCGGCGTCCAGCTACCGGCGAAAATCCAGCAGCCACAGGAGGTTGTa                                                              >  1:789688/1‑140 (MQ=255)                                                                            cggcggTTAAGCGGCCATGCCCATGCCCAGCCCAGCGTGTTACCGAGGCCAGACGGATCGGTGTTATCACGGCGTGCCATCTGGTTGCCTTCCGGCGTCCAGCTACCGGCGAAAATCCAGCAGCCACAGGAGGTTGTAcc                                                            >  1:487664/1‑140 (MQ=255)                                                                                  ttAAGCGGCCATGCCCATGCCCAGCCCAGCGTGTTACCGAGGCCAGACGGATCGGTGTTATCACGGCGTGCCATCTGGTTGCCTTCCGGCGTCCAGCTACCGGCGAAAATCCAGCAGCCACAGGAGGTTGTACCGTCATc                                                      >  2:76556/1‑140 (MQ=255)                                                                                         gCCATGCCCATGCCCAGCCCAGCGTGTTACCGAGGCCAGACGGATCGGTGTTATCACGGCGTGCCATCTGGTTGCCTTCCGGCGTCCAGCTACCGGCGAAAATCCAGCAGCCACAGGAGGTTGTACCGTCATCGCGCAAt                                               >  1:688730/1‑140 (MQ=255)                                                                                           catgcccatgccCAGCCCAGCGTGTTACCGAGGCCAGACGGATCGGTGTTATCACGGCGTGCCATCTGGTTGCCTTCCGGCGTCCAGCTACCGGCGAAAATCCAGCAGCCACAGGAGGTTGTACCGTCATCGCGCAATTg                                             >  2:1234536/1‑140 (MQ=255)                                                                                             tgcccatgcccaGCCCAGCGTGTTACCGAGGCCAGACGGATCGGTGTTATCACGGCGTGCCATCTGGTTGCCTTCCGGCGTCCAGCTACCGGCGAAAATCCAGCAGCCACAGGAGGTTGTACCGTCATCGCGCAATTggg                                           <  2:166304/140‑1 (MQ=255)                                                                                               cccatgcccaGCCCAGCGTGTTACCGAGGCCAGACGGATCGGTGTTATCACGGCGTGCCATCTGGTTGCCTTCCGGCGTCCAGCTACCGGCGAAAATCCAGCAGCCACAGGAGGTTGTACCGTCATCGCGCAATTGGGcg                                         >  2:632277/1‑140 (MQ=255)                                                                                                ccatgcccaGCCCAGCGTGTTACCGAGGCCAGACGGATCGGTGTTATCACGGCGTGCCATCTGGTTGCCTTCCGGCGTCCAGCTACCGGCGAAAATCCAGCAGCCACAGGAGGTTGTACCGTCATCGCGCAATTGGGcga                                        <  1:311436/140‑1 (MQ=255)                                                                                                ccatgcccaGCCCAGCGTGTTACCGAGGCCAGACGGATCGGTGTTATCACGGCGTGCCATCTGGTTGCCTTCCGGCGTCCAGCTACCGGCGAAAATCCAGCAGCCACAGGAGGTTGTACCGTCATCGCGCAATTGGGcga                                        <  2:225500/140‑1 (MQ=255)                                                                                                 catgcccaGCCCAGCGTGTTACCGAGGCCAGACGGATCGGTGTTATCACGGCGTGCCATCTGGTTGCCTTCCGGCGTCCAGCTACCGGCGAAAATCCAGCAGCCACAGGAGGTTGTACCGTCATCGCGCAATTGGGcgaa                                       <  2:979883/140‑1 (MQ=255)                                                                                                 catgcccaGCCCAGCGTGTTACCGAGGCCAGACGGATCGGTGTTATCACGGCGTGCCATCTGGTTGCCTTCCGGCGTCCAGCTACCGGCGAAAATCCAGCAGCCACAGGAGGTTGTACCGTCATCGCGCAATTGGGcgaa                                       <  1:1251789/140‑1 (MQ=255)                                                                                                    gcccagcccaGCGTGTTACCGAGGCCAGACGGATCGGTGTTATCACGGCGTGCCATCTGGTTGCCTTCCGGCGTCCAGCTACCGGTGAAAATCCAGCAGCCACAGGAGGTTGTACCGTCATCGCGCAATTGGGcgaacga                                    >  2:507755/1‑140 (MQ=255)                                                                                                    gcccagcccaGCGTGTTACCGAGGCCAGACGGATCGGTGTTATCACGGCGTGCCATCTGGTTGCCTTCCGGCGTCCAGCTACCGGCGAAAATCCAGCAGCCACAGGAGGTTGTACCGTCATCGCGCAATTGGGcgaacga                                    >  2:47178/1‑140 (MQ=255)                                                                                                     cccagcccagCGTGTTACCGAGGCCAGACGGATCGGTGTTATCACGGCGTGCCATCTGGTTGCCTTCCGGCGTCCAGCTACCGGCGAAAATCCAGCAGCCACAGGAGGTTGTACCGTCATCGCGCAATTGGGcgaacgaa                                   <  2:789688/140‑1 (MQ=255)                                                                                                         gcccagcGTGTTACCGAGGCCAGACGGATCGGTGTTATCACGGCGTGCCATCTGGTTGCCTTCCGGCGTCCAGCTACCGGCGAAAATCCAGCAGCCACAGGAGGTTGTACCGTCATCGCGCAATTGGGCGAACGAACTaa                               >  1:453269/1‑140 (MQ=255)                                                                                                               cGTGTTACCGAGGCCAGACGGATCGGTGTTATCACGGCGTGCCATCTGGTTGCCTTCCGGCGTCCAGCTACCGGCGAAAATCCAGCAGCCACAGGAGGTTGTACCGTCATCGCGCAATTGGGCGAACGAACTAAgttgtt                         >  2:365490/1‑140 (MQ=255)                                                                                                               cGTGTTACCGAGGCCAGACGGATCGGTGTTATCACGGCGTGCCATCTGGTTGCCTTCCGGCGTCCAGCTACCGGCGAAAATCCAGCAGCCACAGGAGGTTGTACCGTCATCGCGCAATTGGGCGAACGAACTAAgttgtt                         >  1:336792/1‑140 (MQ=255)                                                                                                                   ttACCGAGGCCAGACGGATCGGTGTTATCACGGCGTGCCATCTGGTTGCCTTCCGGCGTCCAGCTACCGGCGAAAATCCAGCAGCCACAGGAGGTTGTACCGTCATCGCGCAATTGGGTGAACGAACTAAGTTGTTGGcc                     <  1:1227848/140‑1 (MQ=255)                                                                                                                    tACCGAGGCCAGACGGATCGGTGTTATCACGGCGTGCCATCTGGTTGCCTTCCGGCGTCCAGCTACCGGCGAAAATCCAGTAGCCACAGGAGGTTGTACCGTCATCGCGCAATTGGGCGAACGAACTAAGTTGTTGGCCt                    >  1:18299/1‑140 (MQ=255)                                                                                                                    tACCGAGGCCAGACGGATCGGTGTTATCACGGCGTGCCATCTGGTTGCCTTCCGGCGTCCAGCTACCGGCGAAAATCCAGCAGCCACAGGAGGTTGTACCGTCATCGCGCAATTGGGCGAACGAACTAAGTTGTTGGCCt                    >  2:480755/1‑140 (MQ=255)                                                                                                                    tACCGAGGCCAGACGGATCGGTGTTATCACGGCGTGCCATCTGGTTGCCTTCCGGCGTCCAGCTACCGGCGAAAATCCAGCAGCCACAGGAGGTTGGACCGTCATCGCGCAATTGGGCGAACGAACTAAGTTGTTGGCCt                    >  2:1309078/1‑140 (MQ=255)                                                                                                                       cGAGGCCAGACGGATCGGTGTTATCACGGCGTGCCATCTGGTTGCCTTCCGGCGTCCAGCTACCGGCGAAAATCCAGCAGCCACAGGAGGTTGTACCGTCATCGCGCAATTGGGCGAACGAACTAAGTTGTTGGCCTTTc                 <  1:287212/140‑1 (MQ=255)                                                                                                                           gCCAGACGGATCGGTGTTATCACGGCGTGCCATCTGGTTGCCTTCCGGCGTCCAGCTACCGGCGAAAATCCAGCAGCCACAGGAGGTTGTACCGTCATCGCGCAATTGGGCGAACGAACTAAGTTGTTGGCCTTTCTTGa             >  1:691727/1‑140 (MQ=255)                                                                                                                           gCCAGACGGATCGGTGTTATCACGGCGTGCCATCTGGTTGCCTTCCGGCGTCCAGCTACCGGCGAAAATCCAGCAGCCACAGGAGGTTGTACCGTCATCGCGCAATTGGGCGAACGAACTAAGTTGTTGGCCTTTCTTGa             >  1:1050562/1‑140 (MQ=255)                                                                                                                              aGACGGATCGGTGTTATCACGGCGTGCCATCTGGTTGCCTTCCGGCGTCCAGCTACCGGCGAAAATCCAGCAGCCACAGGAGGTTGTACCGTCATCGCGCAATTGGGCGAACGAACTAAGTTGTTGGCCTTTCTTGACGa          >  2:405941/1‑140 (MQ=255)                                                                                                                                    aTCGGTGTTATCACGGCGTGCCATCTGGTTGCCTTCCGGCGTCCAGCTACCGGCGAAAATCCAGCAGCCACAGGAGGTTGTACCGTCATCGCGCAATTGGGCGAACGAACTAAGTTGTTGGCCTTTCTTGACGATAACCg    >  2:548706/1‑140 (MQ=255)                                                                                                                                     tCGGTGTTATCACGGCGTGCCATCTGGTTGCCTTCCGGCGTCCAGCTACCGGCGAAAATCCAGCAGCCACAGGAGGTTGTACCGTCATCGCGCAATTGGGCGAACGAACTAAGTTGTTGGCCTTTCTTGACGATAACCGc   >  1:363683/1‑140 (MQ=255)                                                                                                                                     tCGGTGTTATCACGGCGTGCCATCTGGTTGCCTTCCGGCGTCCAGCTACCGGCGAAAATCCAGCAGCCACAGGAGGTTGTACCGTCATCGCGCAATTGGGCGAACGAACTAAGTTGTTGGCCTTTCTTGACGATAACCGc   >  2:1169330/1‑140 (MQ=255)                                                                                                                                      cGGTGTTATCACGGCGTGCCATCTGGTTGCCTTCCGGCGTCCAGCTACCGGCGAAAATCCAGCAGCCACAGGAGGTTGTACCGTCATCGCGCAATTGGGCGAACGAACTAAGTTGTTGGCCTTTCTTGACGATAACCGCa  >  2:1349833/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                         TCCCATTTCAGCAACTGACGCTTCGGATCCCACGGGTTACCCTGCGGATCTGCGGAGGCGCGGTTATACAGAATGCGGCGGTTAAGCGGCCATGCCCATGCCCAGCCCAGCGTGTTACCGAGGCCAGACGGATCGGCGTTATCACGGCGTGCCATCTGGTTGCCTTCCGGCGTCCAGCTACCGGCGAAAATCCAGCAGCCACAGGAGGTTGTACCGTCATCGCGCAATTGGGCGAACGAACTAAGTTGTTGGCCTTTCTTGACGATAACCGCA  >  NC_000913/4083418‑4083690

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑