Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,138,631	A→G	G135G (GGT→GGC)	gldA ←	L‑1,2‑propanediol dehydrogenase/glycerol dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,138,631	0	A	G	100.0%	315.2 / NA	89	G135G (GGT→GGC)	gldA	L‑1,2‑propanediol dehydrogenase/glycerol dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (35/54);  total (35/54)

GCACGCGCTTCAAACCA‑GGTT‑GCCAGCGCATCGCCGATACCCGCCGCTAACAGACGTGCAGGTGCGCCAGCGACGATTTTGGTGTCGACAATGACCATATTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCACCCTCATCGGTGTAGATAACAGACAATGCGCTGCACGGTGCATCGGTAGAGGCGATAGTCGGTGCGATCGCTACCGGAACACCCATGAAATGTGCCAGTGCTTTGGCAGTATCGAGGGTTTTTCCGCCACCGAT  >  NC_000913/4138494‑4138765                                                                                                                                            |                                                                                                                                       gCACGCGCTTCAAACCA‑GGTT‑GCCAGCGCATCGCCGATACCCGCCGCTAACAGACGTGCAGGTGCGCCAGCGACGATTTTGGTGTCGACAATGACCATATTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCGcc                                                                                                                                      >  2:729558/1‑140 (MQ=255)  cccgcgCTAAAACCCG‑GGTT‑GCCGGCGCATCGCCGATCCCCGCCGCTAACAGACGTGCAGGTGCGCCAGCGACGATTTTGGTGTCGACAATGACCATATTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCGccc                                                                                                                                     <  2:487133/138‑1 (MQ=255)     gcgcTTCAAACCA‑GGTT‑GCCAGCGCATCGCCGATACCCGCCGCTAACAGACGTGCAGGTGCGCCAGCGACGATTTTGGTGTCGACAATGACCATATTCGGGTTATTTGGCAACAGCAGATAGTGGTCAAACTCGCCCTCa                                                                                                                                  <  1:5106/140‑1 (MQ=255)     ccattcaaaaCCG‑GTTT‑GCCCGCGCCTCGCCAATCCCCGCCGCTAACAGACGTGCAGGTGCGCCAGCGACGATTTTGGTGTCGACAATGACCATATTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCGCCCTCa                                                                                                                                  <  2:1244563/133‑1 (MQ=255)      cccTTAAAACGG‑GGTC‑GCCAGCCCTCCCCCGATCCCCGCCGCTAACAGACGTGCAGGTGCGCCAGCGACGATTTTGGTGTCGACAATGACCATATTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCGCCCTCAt                                                                                                                                 <  1:43166/138‑1 (MQ=255)       cgctCAAACCGGGGTG‑GCCGGCGCACCGCCGATCCCCGCCGCTAACAGACGTGCAGGTGCGCCAGCGACGATTTTGGTGTCGACAATGACCATATTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCGCCCTCAt                                                                                                                                 <  2:372581/137‑1 (MQ=255)         tttaaaCCC‑GGTTCGCCAGCGCACCGCCGATCCCCGCCGCTAACAGACGTGCAGGTGCGCCAGCGACGATTTTGGTGTCGACAATGACCATATTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCGCCCTCATCg                                                                                                                               <  1:756782/137‑1 (MQ=255)         ctCAAACCCGGGTC‑GCCGGCGCACCGCCGATCCCCGCCGCTAACAGACGTGCAGGTGCGCCAGCGACGATTTTGGTGTCGACAATGACCATATTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCGCCCTCATCg                                                                                                                               <  2:655405/139‑1 (MQ=255)          tCAAACCA‑GGTT‑GCCAGCGCATCGCCGATACCCGCCGCTAACAGACGTGCAGGTGCGCCAGCGACGATTTTGGTGTCGACAATGACCATATTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCGCCCTCATCGgt                                                                                                                             <  1:889158/140‑1 (MQ=255)          aaaaccga‑GGTG‑GCCAGCGCACCGCCGATACCCGCCGCTAACAGACGTGCAGGTGCGCCAGCGACGATTTTGGTGTCGACAATGACCATATTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCGCCCTCATCGgt                                                                                                                             <  1:19441/133‑1 (MQ=255)           cAAACCA‑GGTT‑GCCAGCGCATCGCCGATACCCGCCGCTAACAGACGTGCAGGTGCGCCAGCGACGATTTTGGTGTCGACAATGACCATATTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCGCCCTCATCGgtg                                                                                                                            >  1:582353/1‑140 (MQ=255)            aaaCCG‑GGTT‑GCCAGCGCCCCGCCGATCCCCGCCGCTAACAGACGTGCAGGTGCGCCAGCGACGATTTTGGTGTCGACAATGACCATATTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCGCCCTCATCGgtgt                                                                                                                           <  2:518805/140‑1 (MQ=255)            aaaCCG‑GGTC‑CCCAGCGCACCGCCGATCCCCGCCGCTAACAGACGTGCAGGTGCGCCAGCGACGATTTTGGTGTCGACAATGACCATATTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCGCCCTCATCGgtgt                                                                                                                           <  2:329421/140‑1 (MQ=255)                          cAGCGCATCGCCGATACCCGCCGCTAACAGACGTGCAGGTGCGCCAGCGACGATTTTGGTGTCGACAATGACCATATTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCGCCCTCATCGGTGTAGATAACAGatg                                                                                                               >  1:974569/1‑138 (MQ=255)                             ccccgcGCCGATCCCCGCCGCTAACAGACGTGCAGGTGCGCCAGCGACGATTTTGGTGTCGACAATGACCATATTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCGCCCTCATCGGTGTAGATAACAGACAATg                                                                                                            <  2:854508/135‑1 (MQ=255)                               ccgcgccgcTCCCCGCCGCTAACAGACGTGCAGGTGCGCCAGCGACGATTTTGGTGTCGACAATGACCATATTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCGCCCTCATCGGTGTAGATAACAGACAATgcg                                                                                                          <  2:934642/137‑1 (MQ=255)                               cATCGCCGATACCCGCCGCTAACAGACGTGCAGGTGCGCCAGCGACGATTTTGGTGTCGACAATGACCATATTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCGCCCTCATCGGTGTAGATAACAGACAATgcg                                                                                                          >  1:384171/1‑140 (MQ=255)                                aTCGCCGATACCCGCCGCTAACAGACGTGCAGGTGCGCCAGCGACGATTTTGGTGTCGACAATGACCATATTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCGCCCTCATCGGTGTAGATAACAGACAATgcgc                                                                                                         >  1:764787/1‑140 (MQ=255)                                  cGCCTATACCCGCCGCTAACAGACGTGCAGGTGCGCCAGCGACGATTTTGGTGTCGACAATGACCATATTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCGCCCTCATCGGTGTAGATAACAGACAATGCGCTg                                                                                                       <  2:355781/140‑1 (MQ=255)                                   gCCGATACCCGCCGCTAACAGACGTGCAGGTGCGCCAGCGACGATTTTGGTGTCGACAATGACCATATTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCGCCCTCATCGGTGTAGATAACAGACAATGCGCTGc                                                                                                      >  2:1403055/1‑140 (MQ=255)                                    cggttccccGCCGCTAACAGACGTGCAGGTGCGCCAGCGACGATTTTGGTGTCGACAATGACCATATTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCGCCCTCATCGGTGTAGATAACAGACAATGCGCTGCa                                                                                                     <  1:898450/134‑1 (MQ=255)                                    ccGATCCCCGCCGCTAACAGACGTGCAGGTGCGCCAGCGACGATTTTGGTGTCGACAATGACCATATTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCGCCCTCATCGGTGTAGATAACAGACAATGCGCTGCa                                                                                                     <  1:461531/140‑1 (MQ=255)                                    ccGATACCCGCCGCTAACAGACGTGCAGGTGCGCCAGCGACGATTTTGGTGTCGACAATGACCATATTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCGCCCTCATCGGTGTAGATAACAGACAATGCGCTGCa                                                                                                     <  1:516527/140‑1 (MQ=255)                                    ccGATACCCGCCGCTAACAGACGTGCAGGTGCGCCAGCGACGATTTTGGTGTCGACAATGACCATATTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCGCCCTCATCGGTGTAGATAACAGACAATGCGCTGCa                                                                                                     <  2:919323/140‑1 (MQ=255)                                     cgacccccGCCGCTAACAGACGTGCAGGTGCGCCAGCGACGATTTTGGTGTCGACAATGACCATATTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCGCCCTCATCGGTGTAGATAACAGACAATGCGCTGCAc                                                                                                    <  2:186316/135‑1 (MQ=255)                                     cGATACCCGCCGCTAACAGACGTGCAGGTGCGCCAGCGACGATTTTGGTGTCGACAATGACCATATTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCGCCCTCATCGGTGTAGATAACAGACAATGCGCTGCAc                                                                                                    <  2:384171/140‑1 (MQ=255)                                      gATACCCGCCGCTAACAGACGTGCAGGTGCGCCAGCGACGATTTTGGTGTCGACAATGACCATATTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCGCCCTCATCGGTGTAGATAACAGACAATGCGCTGCACg                                                                                                   <  2:993539/140‑1 (MQ=255)                                      aacccccGCCGCTAACAGACGTGCAGGTGCGCCAGCGACGATTTTGGTGTCGACAATGACCATATTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCGCCCTCATCGGTGTAGATAACAGACAATGCGCTGCACg                                                                                                   <  1:1376097/136‑1 (MQ=255)                                       aTACCCGCCGCTAACAGACGTGCAGGTGCGCCAGCGACGATTTTGGTGTCGACAATGACCATATTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCGCCCTCATCGGTGTAGATAACAGACAATGCGCTGCACgg                                                                                                  <  2:1290033/140‑1 (MQ=255)                                          cccGCCGCTAACAGACGTGCAGGTGCGCCAGCGACGATTTTGGTGTCGACAATGACCATATTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCGCCCTCATCGGTGTAGATAACAGACAATGCGCTGCACGGTGc                                                                                               <  2:1253724/140‑1 (MQ=255)                                            cgccgcTAACAGACGTGCAGGTGCGCCAGCGACGATTTTGGTGTCGACAATGACCATATTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCGCCCTCATCGGTGTAGATAACAGACAATGCGCTGCACGGTGCAt                                                                                             >  1:1254243/1‑140 (MQ=255)                                               cgcTAACAGACGTGCAGGTGCGCCAGCGACGATTTTGGTGTCGACAATGACCATATTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCGCCCTCATCGGTGTAGATAACAGACAATGCGCTGCACGGTGCATCgg                                                                                          >  1:1249683/1‑140 (MQ=255)                                                 cTAACAGACGTGCAGGTGCGCCAGCGACGATTTTGGTGTCGACAATGACCATATTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCGCCCTCATCGGTGTAGATAACAGACAATGTGCTGCACGGTGCATCGGTa                                                                                        <  2:831321/140‑1 (MQ=255)                                                 cTAACAGACGTGCAGGTGCGCCAGCGACGATTTTGGTGTCGACAATGACCATATTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCGCCCTCATCGGTGTAGATAACAGACAATGCGCTGCACGGTGCATCGGTa                                                                                        <  1:1043573/140‑1 (MQ=255)                                                 cTAACAGACGTGCAGGTGCGCCAGCGACGATTTTGGTGTCGACAATGACCATATTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCGCCCTCATCGGTGTAGATAACAGACAATGCGCTGCACGGTGCATCGGTa                                                                                        <  2:817665/140‑1 (MQ=255)                                                      aGACGTGCAGGTGCGCCAGCGACGATTTTGGTGTCGACAATGACCATATTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCGCCCTCATCGGTGTAGATAACAGACAATGCGCTGCACGGTGCATCGGTAGAGGc                                                                                   <  2:1254243/140‑1 (MQ=255)                                                         cGTGCAGGTGCGCCAGCGACGATTTTGGTGTCGACAATGACCATATTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCGCCCTCATCGGTGTAGATAACAGACAATGCGCTGCACGGTGCATCGGTAGAGGCGAt                                                                                >  2:958731/1‑140 (MQ=255)                                                           tGCAGGTGCGCCAGCGACGATTTTGGTGTCGACAATGACCATATTCGGGTTATTTGGTAACAGCAGATAGCGGTCAAACTCGCCCTCATCGGTGTAGATAACAGACAATGCGCTGCACGGCGCATCGGTAGAGGCGATAg                                                                              >  1:392220/1‑140 (MQ=255)                                                           tGCAGGTGCGCCAGCGACGATTTTGGTGTCGACAATGACCATATTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCGCCCTCATCGGTGTAGATAACAGACAATGCGCTGCACGGTGCATCGGTAGAGGCGATAg                                                                              >  1:534758/1‑140 (MQ=255)                                                           tGCAGGTGCGCCAGCGACGATTTTGGTGTCGACAATGACCATATTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCGCCCTCATCGGTGTAGATAACAGACAATGCGCTGCACGGTGCATCGGTAGAGGCGATAg                                                                              >  2:756149/1‑140 (MQ=255)                                                                gTGCGCCAGCGACGATTTTGGTGTCGACAATGACCATATTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCGCCCTCATCGGTGTAGATAACAGACAATGCGCTGCACGGTGCATCGGTAGAGGCGATAGTCGGt                                                                         <  2:862896/140‑1 (MQ=255)                                                                 tGCGCCAGCGACGATTTTGGTGTCGACAATGACCATATTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCGCCCTCATCGGTGTAGATAACAGACAATGCGCTGCACGGTGCATCGGTAGAGGCGATAGTCGGTg                                                                        <  1:154425/140‑1 (MQ=255)                                                                  gcgcCAGCGACGATTTTGGTGTCGACAATGACCATATTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCGCCCTCATCGGTGTAGATAACAGACAATGCGCTGCACGGTGCATCGGTAGAGGCGATAGTCGGTGc                                                                       >  1:619441/1‑140 (MQ=255)                                                                     ccAGCGACGATTTTGGTGTCGACAATGACCATATTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCGCCCTCATCGGTGTAGATAACAGACAATGCGCTGCACGGTGCATCGGTAGAGGCGATAGTCGGTGCGAt                                                                    <  2:299823/140‑1 (MQ=255)                                                                     ccAGCGACGATTTTGGTGTCGACAATGACCATATTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCGCCCTCATCGGTGTAGATAACAGACAATGCGCTGCACGGTGCATCGGTAGAGGCGATAGTCGGTGCGAt                                                                    <  1:1403055/140‑1 (MQ=255)                                                                     ccAGCGACGATTTTGGTGTCGACAATGACCATATTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCGCCCTCATCGGTGTAGATAACAGACAATGCGCTGCACGGTGCATCGGTAGAGGCGATAGTCGGTGCGAt                                                                    <  2:53442/140‑1 (MQ=255)                                                                     ccAGCGACGATTTTGGTGTCGACAATGACCATATTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCGCCCTCATCGGTGTAGATAACAGACAATGCGCTGCACGGTGCATCGGTAGAGGCGATAGTCGGTGCGAt                                                                    <  2:582353/140‑1 (MQ=255)                                                                     ccAGCGACGATTTTGGTGTCGACAATGACCATATTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCGCCCTCATCGGTGTAGATAACAGACAATGCGCTGCACGGTGCATCGGTAGAGGCGATAGTCGGTGCGAt                                                                    <  2:487397/140‑1 (MQ=255)                                                                     ccAGCGACGATTTTGGTGTCGACAATGACCATATTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCGCCCTCATCGGTGTAGATAACAGACAATGCGCTGCACGGTGCATCGGTAGAGGCGATAGTCGGTGCGAt                                                                    <  2:619441/140‑1 (MQ=255)                                                                     ccAGCGACGATTTTGGTGTCGACAATGACCATATTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCGCCCTCATCGGTGTAGATAACAGACAATGCGCTGCACGGTGCATCGGTAGAGGCGATAGTCGGTGCGAt                                                                    <  1:117104/140‑1 (MQ=255)                                                                      cAGCGACGATTTTGGTGTCGACAATGACCATATTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCGCCCTCATCGGTGTAGATAACAGACAATGCGCTGCACGGTGCATCGGTAGAGGCGATAGTCGGTGCGATc                                                                   <  2:1380351/140‑1 (MQ=255)                                                                        gCGACGATTTTGGTGTCGACAATGACCATATTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCGCCCTCATCGGTGTAGATAACAGACAATGCGCTGCACGGTGCATCGGTAGAGGCGATAGTCGGTGCGATCGc                                                                 >  2:1340925/1‑140 (MQ=255)                                                                        gCGACGATTTTGGTGTCGACAATGACCATATTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCGCCCTCATCGGTGTAGATAACAGACAATGCGCTGCACGGTGCATCGGTAGAGGCGATAGTCGGTGCGATCGc                                                                 <  1:143522/140‑1 (MQ=255)                                                                          gacgaTTTTGGTGTCGACAATGACCATATTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCGCCCTCATCGGTGTAGATAACAGACAATGCGCTGCACGGTGCATCGGTAGAGGCGATAGTCGGTGCGATCGCTa                                                               >  1:1279842/1‑140 (MQ=255)                                                                              aTTTTGGTGTCGACAATGACCATATTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCGCCCTCATCGGTGTAGATAACAGACAATGCGCTGCACGGTGCATCGGTAGAGGCGATAGTCGGTGCGATCGCTACCgg                                                           >  2:1399328/1‑140 (MQ=255)                                                                                tttGGTGTCGACAATGACCATATTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCGCCCTCATCGGTGTAGATAACAGACAATGCGCTGCACGGTGCATCGGTAGAGGCGATAGTCGGTGCGATCGCTACCGGaa                                                         <  1:219859/140‑1 (MQ=255)                                                                                tttGGTGTCGACAATGACCATATTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCGCCCTCATCGGTGTAGATAACAGACAATGCGCTGCACGGTGCATCGGTAGAGGCGATAGTCGGTGCGATCGCTACCGGaa                                                         <  1:320938/140‑1 (MQ=255)                                                                                tttGGTGTCGACAATGACCATATTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCGCCCTCATCGGTGTAGATAACAGACAATGCGCTGCACGGTGCATCGGTAGAGGCGATAGTCGGTGCGATCGCTACCGGaa                                                         <  2:534758/140‑1 (MQ=255)                                                                                  tGGTGTCGACAATGACCATATTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCGCCCTCATCGGTGTAGATAACAGACAATGCGCTGCACGGTGCATCGGTAGAGGCGATAGTCGGTGCGATCGCTACCGGAaca                                                       <  1:729558/140‑1 (MQ=255)                                                                                         gACAATGACCATATTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCGCCCTCATCGGTGTAGATAACAGACAATGCGCTGCACGGTGCATCGGTAGAGGCGATAGTCGGTGCGATCGCTACCGGAACACCCATGa                                                >  1:239958/1‑140 (MQ=255)                                                                                           cAATGACCATATTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCGCCCTCATCGGTGTAGATAACAGACAATGCGCTGCACGGTGCATCGGTAGAGGCGATAGTCGGTGCGATCGCTACCGGAACACCCATGaaa                                              <  2:615257/140‑1 (MQ=255)                                                                                                 ccATATTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCGCCCTCATCGGTGTAGATAACAGACAATGCGCTGCACGGTGCATCGGTAGAGGCGATAGTCGGTGCGATCGCTACCGGAACACCCATGAAATGTGcc                                        >  1:772576/1‑140 (MQ=255)                                                                                                 ccATATTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCGCCCTCATCGGTGTAGATAACAGACAATGCGCTGCACGGTGCATCGGTAGAGGCGATAGTCGGTGCGATCGCTACCGGAACACCCATGAAATGTGcc                                        <  2:1167784/140‑1 (MQ=255)                                                                                                   atatTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCGCCCTCATCGGTGTAGATAACAGACAATGCGCTGCACGGTGCATCGGTAGAGGCGATAGTCGGTGCGATCGCTACCGGAACACCCATGAAATGTGCCAg                                      <  2:239958/140‑1 (MQ=255)                                                                                                   atatTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCGCCCTCATCGGTGTAGATAACAGACAATGCGCTGCACGGTGCATCGGTAGAGGCGATAGTCGGTGCGATCGCTACCGGAACACCCATGAAATGTGCCAg                                      <  2:1246364/140‑1 (MQ=255)                                                                                                     atTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCGCCCTCATCGGTGTAGATAACAGACAATGCGCTGCACGGTGCATCGGTAGAGGCGATAGTCGGTGCGATCGCTACCGGAACACCCATGAAATGTGCCAGTg                                    >  1:658207/1‑140 (MQ=255)                                                                                                     atTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCGCCCTCATCGGTGTAGATAACAGACAATGCGCTGCACGGTGCATCGGTAGAGGCGATAGTCGGTGCGATCGCTACCGGAACACCCATGAAATGTGCCAGTg                                    >  1:780263/1‑140 (MQ=255)                                                                                                        cGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCGCCCTCATCGGTGTAGATAACAGACAATGCGCTGCACGGTGCATCGGTAGAGGCGATAGTCGGTGCGATCGCTACCGGAACACCCATGAAATGTGCCAGTGCtt                                 <  1:1161218/140‑1 (MQ=255)                                                                                                        cGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCGCCCTCATCGGTGTAGATAACAGACAATGCGCTGCACGGTGCATCGGTAGAGGCGATAGTCGGTGCGATCGCTACCGGAACACCCATGAAATGTGCCAGTGCtt                                 <  1:1340925/140‑1 (MQ=255)                                                                                                          ggTTATTTGGCAACAGCAGATAGCGGTCAAACTCGCCCTCATCGGTGTAGATAACAGACAATGCGCTGCACGGTGCATCGGTAGAGGCGATAGTCGGTGCGATCGCTACCGGAACACCCATGAAATGTGCCAg                                      <  1:114008/133‑1 (MQ=255)                                                                                                          ggTTATTTGGCAACAGCAGATAGCGGTCAAACTCGCCCTCATCGGTGTAGATAACAGACAATGCGCTGCACGGTGCATCGGTAGAGGCGATAGTCGGTGCGATCGCTACCGGAACACCCATGAAATGTGCCAg                                      >  2:114008/1‑133 (MQ=255)                                                                                                           gTTATTTGGCAACAGCAGATAGCGGTCAAACTCGCGCTCATCGGTGTAGATAACAGACAATGCGCTGCACGGTGCATCGGTAGAGGCGATAGTCGGTGCGATCGCTACCGGAACACCCATGAAATGTGCCAGTGCTTTgg                              <  1:575104/140‑1 (MQ=255)                                                                                                                          cagATAGCGGTCAAACTCGCCCTCATCGGTGTAGATAACAGACAATGCGCTGCACGGTGCATCGGTAGAGGCGATAGTCGGTGCGATCGCTACCGGAACACCCATGAAATGTGCCAGTGCTTTGGCAGTATCGAGGGttt               >  2:744093/1‑140 (MQ=255)                                                                                                                          cagATAGCGGTCAAACTCGCCCTCATCGGTGTAGATAACAGACAATGCGCTACACGGTGCATCGGTAGAGGCGATAGTCGGTGCGATCGCTACCGGAACACCCATGAAATGTGCCAGTGCTTTGGCAGTATCGAGGGttt               >  1:930123/1‑140 (MQ=255)                                                                                                                               aGCGGTCAAACTCGCCCTCATCGGTGTAGATAACAGACAATGCGCTGCACGGTGCATCGGTAGAGGCGATAGTCGGTGCGATCGCTACCGGAACACCCATGAAATGTGCCAGTGCTTTGGCAGTATCGAGGGTTTTTCCg          >  1:1391634/1‑140 (MQ=255)                                                                                                                                 cGGTCAAACTCGCCCTCATCGGTGTAGATAACAGACAATGCGCTGCACGGTGCATCGGTAGAGGCGATAGTCGGTGCGATCGCTACCGGAACACCCATGAAATGTGCCAGTGCTTTGGCAGTATCGAGGGTTTTTCCGcc        >  2:998360/1‑140 (MQ=255)                                                                                                                                  ggTCAAACTCGCCCTCATCGGTGTAGATAACAGACAATGCGCTGCACGGTGCATCGGTAGAGGCGATAGTCGGTGCGATCGCTACCGGAACACCCATGAAATGTGCCAGTGCTTTGGCAGTATCGAGGGt                 <  2:605721/130‑1 (MQ=255)                                                                                                                                  ggTCAAACTCGCCCTCATCGGTGTAGATAACAGACAATGCGCTGCACGGTGCATCGGTAGAGGCGATAGTCGGTGCGATCGCTACCGGAACACCCATGAAATGTGCCAGTGCTTTGGCAGTATCGAGGGt                 >  1:605721/1‑130 (MQ=255)                                                                                                                                  ggTCAAACTCGCCCTCATCGGTGTAGATAACAGACAATGCGCTGCACGGTGCATCGGTAGAGGCGATAGTCGGTGCGATCGCTACCGGAACACCCATGAAATGTGCCAGTGCTTTGGCAGTATCGAGGGTTTTTCCGCCa       <  2:1081732/140‑1 (MQ=255)                                                                                                                                   gTCAAACTCGCCCTCATCGGTGTAGATAACAGACAATGCGCTGCACGGTGCATCGGTAGAGGCGATAGTCGGTGCGATCGCTACCGGAACACCCATGAAATGTGCCAGTGCTTTGGCAGTATCGAGGGTTTTTCCGCCAc      >  1:800727/1‑140 (MQ=255)                                                                                                                                   gTCAAACTCGCCCTCATCGGTGTAGATAACAGACAATGCGCTGCACGGTGCATCGGTAGAGGCGATAGTCGGTGCGATCGCTACCGGAACACCCATGAAATGTGCCAGTGCTTTGGCAGTATCGAGGGTTTTTCCGCCAc      <  1:786756/140‑1 (MQ=255)                                                                                                                                    tCAAACTCGCCCTCATCGGTGTAGATAACAGACAATGCGCTGCACGGTGCATCGGTAGAGGCGATAGTCGGTGCGATCGCTACCGGAACACCCATGAAATGTGCCAGTGCTTTGGCAGTATCGAGGGTTTTTCCGCCAcc     >  2:705773/1‑140 (MQ=255)                                                                                                                                    tCAAACTCGCCCTCATCGGTGTAGATAACAGACAATGCGCTGCACGGTGCATCGGTAGAGGCGATAGTCGGTGCGATCGCTACCGGAACACCCATGAAATGTGCCAGTGCTTTGGCAGTATCGAGGGTTTTTCCGCCAcc     >  2:550797/1‑140 (MQ=255)                                                                                                                                    tCAAACTCGCCCTCATCGGTGTAGATAACAGACAATGCGCTGCACGGTGCATCGGTAGAGGCGATAGTCGGTGCGATCGCTACCGGAACACCCATGAAATGTGCCAGTGCTTTGGCAGTATCGAGGGTTTTTCCGCCAcc     >  2:24898/1‑140 (MQ=255)                                                                                                                                    tCAAACTCGCCCTCATCGGTGTAGATAACAGACAATGCGCTGCACGGTGCATCGGTAGAGGCGATAGTCGGTGCGATCGCTACCGGAACACCCATGAAATGTGCCAGTGCTTTGGCAGTATCGAGGGTTTTTCCGCCAcc     >  2:183987/1‑140 (MQ=255)                                                                                                                                    tCAAACTCGCCCTCATCGGTGTAGATAACAGACAATGCGCTGCACGGTGCATCGGTAGAGGCGATAGTCGGTGCGATCGCTACCGGAACACCCATGAAATGTGCCAGTGCTTTGGCAGTATCGAGGGTTTTTCCGCCAcc     >  1:1006229/1‑140 (MQ=255)                                                                                                                                      aaaCTCGCCCTCATCGGTGTAGATAACAGACAATGCGCTGCACGGTGCATCGGTAGAGGCGATAGTCGGTGCGATCGCTACCGGAACACCCATGAAATGTGCCAGTGCTTTGGCAGTATCGAGGGTTTTTCCGCCACCGa   >  2:142108/1‑140 (MQ=255)                                                                                                                                      aaaCTCGCCCTCATCGGTGTAGATAACAGACAATGCGCTGCACGGTGCATCGGTAGAGGCGATAGTCGGGGCGATCGCTACCGGAACACCCGTGAAATGTGCCAGTGCTTTGGCAGTATCGAGGGATTTTCCGCCACCGa   >  2:535209/1‑140 (MQ=255)                                                                                                                                       aaCTCGCCCTCATCGGTGTAGATAACAGACAATGCGCTGCACGGTGCATCGGTAGAGGCGATAGTCGGTGCGATCGCTACCGGAACACCCATGAAATGTGCCAGTGCTTTGGCAGTATCGAGGGTTTTTCCGCCACCGAt  >  2:752087/1‑140 (MQ=255)                                                                                                                                            |                                                                                                                                       GCACGCGCTTCAAACCA‑GGTT‑GCCAGCGCATCGCCGATACCCGCCGCTAACAGACGTGCAGGTGCGCCAGCGACGATTTTGGTGTCGACAATGACCATATTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCACCCTCATCGGTGTAGATAACAGACAATGCGCTGCACGGTGCATCGGTAGAGGCGATAGTCGGTGCGATCGCTACCGGAACACCCATGAAATGTGCCAGTGCTTTGGCAGTATCGAGGGTTTTTCCGCCACCGAT  >  NC_000913/4138494‑4138765

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑