Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,240,867	A→G	V463A (GTC→GCC)	xylE ←	D‑xylose:H(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,240,867	0	A	G	100.0%	264.3 / NA	75	V463A (GTC→GCC)	xylE	D‑xylose:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (30/45);  total (30/45)

AAAAAGTCAGCACGCCGAAATGGCGCGGCGTGCTGGACAGGAAGATTACAGCGTAGCAGTTTGTTGTGTTTTCTTCGTTTCCGGTTCCCAGAGCGCTTCCAGCTCCTCAAGGGTTTTACCTTTGGTTTCCGGGACAAATTTCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCAGGAGACGAAGTAGTTC  >  NC_000913/4240734‑4241001                                                                                                                                      |                                                                                                                                       aaaaaGTCAGCACGCCGAAATGGCGCGGCGTGCTGGACAGGAAGATTACAGCGTAGCAGTTTGTTGTGTTTTCTTCGTTTCCGGTTCCCAGAGCGCTTCCAGCTCCTCAAGGGTTTTACCTTTGGTTTCCGGGGCAAAtt                                                                                                                                  <  1:1069651/140‑1 (MQ=255) aaaaaGTCAGCACGCCGAAATGGCGCGGCGTGCTGGACAGGAAGATTACAGCGTAGCAGTTTGTTGTGTTTTCTTCGTTTCCGGTTCCCAGAGCGCTTCCAGCTCCTCAAGGGTTTTACCTTTGGTTTCCGGGGCAAAtt                                                                                                                                  <  1:707149/140‑1 (MQ=255)      gTCAGCACGCCGAAATGGCGCGGCGTGCTGGACAGGAAGATTACAGCGTAGCAGTTTGTTGTGTTTTCTTCGTTTCCGGTTCCCAGAGCGCTTCCAGCTCCTCAAGGGTTTTACCTTTGGTTTCCGGGGCAAATTTCcac                                                                                                                             <  2:311242/140‑1 (MQ=255)       tCAGCACGCCGAAATGGCGCGGCGTGCTGGACAGGAAGATTACAGCGTAGCAGTTTGTTGTGTTTTCTTCGTTTCCGGTTCCCAGAGCGCTTCCAGCTCCTCAAGGGTTTTACCTTTGGTTTCCGGGGCAAATTTCcac                                                                                                                             >  2:794279/1‑139 (MQ=255)       tCAGCACGCCGAAATGGCGCGGCGTGCTGGACAGGAAGATTACAGCGTAGCAGTTTGTTGTGTTTTCTTCGTTTCCGGTTCCCAGAGCGCTTCCAGCTCCTCAAGGGTTTTACCTTTGGTTTCCGGGGCAAATTTCcac                                                                                                                             <  1:794279/139‑1 (MQ=255)        cAGCACGCCGAAATGGCGCGGCGTGCTGGACAGGAAGATTACAGCGTAGCAGTTTGTTGTGTTTTCTTCGTTTCCGGTTCCCAGAGCGCTTCCAGCTCCTCAAGGGTTTTACCTTTGGTTTCCGGGGCAAATTTCCACAt                                                                                                                           >  1:915144/1‑140 (MQ=255)          gCACGCCGAAATGGCGCGGCGTGCTGGACAGGAAGATTACAGCGTAGCAGTTTGTTGTGTTTTCTTCGTTTCCGGTTCCCAGAGCGCTTCCAGCTCCTCAAGGGTTTTACCTTTGGTTTCCGGGGCAAATTTCCACATaa                                                                                                                         >  2:1255546/1‑140 (MQ=255)          gCACGCCGAAATGGCGCGGCGTGCTGGACAGGAAGATTACAGCGTAGCAGTTTGTTGTGTTTTCTTCGTTTCCGGTTCCCAGAGCGCTTCCAGCTCCTCAAGGGTTTTACCTTTGGTTTCCGGGGCAAATTTCCACATaa                                                                                                                         <  1:678094/140‑1 (MQ=255)          gCACGCCGAAATGGCGCGGCGTGCTGGACAGGAAGATTACAGCGTAGCAGTTTGTTGTGTTTTCTTCGTTTCCGGTTCCCAGAGCGCTTCCAGCTCCTCAAGGGTTTTACCTTTGGTTTCCGGGGCAAATTTCCACATaa                                                                                                                         <  2:727514/140‑1 (MQ=255)          gCACGCCGAAATGGCGCGGCGTGCTGGACAGGAAGATTACAGCGTAGCAGTTTGTTGTGTTTTCTTCGTTTCCGGTTCCCAGAGCGCTTCCAGCTCCTCAAGGGTTTTACCTTTGGTTTCCGGGGCAAATTTCCACATaa                                                                                                                         <  1:878667/140‑1 (MQ=255)            aCGCCGAAATGGCGCGGCGTGCTGGACAGGAAGATTACAGCGTAGCAGTTTGTTGTGTTTTCTTCGTTTCCGGTTCCCAGAGCGCTTCCAGCTCCTCAAGGGTTTTACCTTTGGTTTCCGGGGCAAATTTCCACATAAAc                                                                                                                       <  2:56047/140‑1 (MQ=255)                cGAAATGGCGCGGCGTGCTGGACAGGAAGATTACAGCGTAGCAGTTTGTTGTGTTTTCTTCGTTTCCGGTTCCCAGAGCGCTTCCAGCTCCTCAAGGGTTTTACCTTTGGTTTCCGGGGCAAATTTCCACATAAACAGtg                                                                                                                   <  1:372458/140‑1 (MQ=255)                     tGGCGCGGCGTGCTGGACAGGAAGATTACAGCGTAGCAGTTTGTTGTGTTTTCTTCGTTTCCGGTTCCCAGAGCGCTTCCAGCTCCTCAAGGGTTTTACCTTTGGTTTCCGGGGCAAATTTCCACATAAACAGTGCTGcc                                                                                                              >  1:969776/1‑140 (MQ=255)                       gcgcGGCGTGCTGGACAGGAAGATTACAGCGTAGCAGTTTGTTGTGTTTTCTTCGTTTCCGGTTCCCAGAGCGCTTCCAGCTCCTCAAGGGTTTTACCTTTGGTTTCCGGGGCAAATTTCCACATAAACAGTGCTGCCAg                                                                                                            <  2:311658/140‑1 (MQ=255)                        cgcgGCGTGCTGGACAGGAAGATTACAGCGTAGCAGTTTGTTGTGTTTTCTTCGTTTCCGGTTCCCAGAGCGCTTCCAGCTCCTCAAGGGTTTTACCTTTGGTTTCCGGGGCAAATTTCCACATAAACAGTGCTGCCAGa                                                                                                           <  1:765953/140‑1 (MQ=255)                        cgcgGCGTGCTGGACAGGAAGATTACAGCGTAGCAGTTTGTTGTGTTTTCTTCGTTTCCGGTTCCCAGAGCGCTTCCAGCTCCTCAAGGGTTTTACCTTTGGTTTCCGGGGCAAATTTCCACATAAACAGTGCTGCCAGa                                                                                                           <  2:64537/140‑1 (MQ=255)                        cgcgGCGTGCTGGACAGGAAGATTACAGCGTAGCAGTTTGTTGTGTTTTCTTCGTTTCCGGTTCCCAGAGCGCTTCCAGCTCCTCAAGGGTTTTACCTTTGGTTTCCGGGGCAAATTTCCACATAAACAGTGCTGCCAGa                                                                                                           >  2:765953/1‑140 (MQ=255)                        cgcgGCGTGCTGGACAGGAAGATTACAGCGTAGCAGTTTGTTGTGTTTTCTTCGTTTCCGGTTCCCAGAGCGCTTCCAGCTCCTCAAGGGTTTTACCTTTGGTTTCCGGGGCAAATTTCCACATAAACAGTGCTGCCAGa                                                                                                           >  2:72024/1‑140 (MQ=255)                        cgcgGCGTGCTGGACAGGAAGATTACAGCGTAGCAGTTTGTTGTGTTTTCTTCGTTTCCGGTTCCCAGAGCGCTTCCAGCT‑CTCAAGGGTTTTACCTTTGGTTTCCGGGGCAAATTTCCACATAAACAGTGCTGCCAg                                                                                                            >  1:622499/1‑138 (MQ=255)                        cgcgGCGTGCTGGACAGGAAGATTACAGCGTAGCAGTTTGTTGTGTTTTCTTCGTTTCCGGTTCCCAGAGCGCTTCCAGCT‑CTCAAGGGTTTTACCTTTGGTTTCCGGGGCAAATTTCCACATAAACAGTGCTGCCAg                                                                                                            <  2:622499/138‑1 (MQ=255)                         gcggcgTGCTGGACAGGAAGATTACAGCGTAGCAGTTTGTTGTGTTTTCTTCGTTTCCGGTTCCCAGAGCGCTTCCAGCTCCTCAAGGGTTTTACCTTTGGTTTCCGGGGCAAATTTCCACATAAACAGTGCTGCCAGaa                                                                                                          <  2:885869/140‑1 (MQ=255)                          cggcgTGCTGGACAGGAAGATTACAGCGTAGCAGTTTGTTGTGTTTTCTTCGTTTCCGGTTCCCAGAGCGCTTCCAGCTCCTCAAGGGTTTTACCTTTGGTTTCCGGGGCAAATTTCCACATAAACAGTGCTGCCAGAAc                                                                                                         <  2:1065183/140‑1 (MQ=255)                             cgTGCTGGACAGGAAGATTACAGCGTAGCAGTTTGTTGTGTTTTCTTCGTTTCCGGTTCCCAGAGCGCTTCCAGCTCCTCAAGGGTTTTACCTTTGGTTTCCGGGGCAAATTTCCACATAAACAGTGCTGCCAGAACGcc                                                                                                      >  1:1237876/1‑140 (MQ=255)                               tGCTGGACAGGAAGATTACAGCGTAGCAGTTTGTTGTGTTTTCTTCGTTTCCGGTTCCCAGAGCGCTTCCAGCTCCTCAAGGGTTTTACCTTTGGTTTCCGGGGCAAATTTCCACATAAACAGTGCTGCCAGAACGCCCa                                                                                                    >  1:992389/1‑140 (MQ=255)                                 cTGGACAGGAAGATTACAGCGTAGCAGTTTGTTGTGTTTTCTTCGTTTCCGGTTCCCAGAGCGCTTCCAGCTCCTCAAGGGTTTTACCTTTGGTTTCCGGGGCAAATTTCCACATAAACAGTGCTGCCAGAACGCCCATa                                                                                                  <  1:265116/140‑1 (MQ=255)                                  tGGACAGGAAGATTACAGCGTAGCAGTTTGTTGTGTTTTCTTCGTTTCCGGTTCCCAGAGCGCTTCCAGCTCCTCAAGGGTTTTACCTTTGGTTTCCGGGGCAAATTTCCACATAAACAGTGCTGCCAGAACGCCCATAc                                                                                                 <  2:905796/140‑1 (MQ=255)                                  tGGACAGGAAGATTACAGCGTAGCAGTTTGTTGTGTTTTCTTCGTTTCCGGTTCCCAGAGCGCTTCCAGCTCCTCAAGGGTTTTACCTTTGGTTTCCGGGGCAAATTTCCACATAAACAGTGCTGCCAGAACGCCCATAc                                                                                                 <  1:1275953/140‑1 (MQ=255)                                        ggAAGATTACAGCGTAGCAGTTTGTTGTGTTTTCTTCGTTTCCGGTTCCCAGAGCGCTTCCAGCTCCTCAAGGGTTTTACCTTTGGTTTCCGGGGCAAATTTCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGt                                                                                           <  1:411922/140‑1 (MQ=255)                                         gAAGATTACAGCGTAGCAGTTTGTTGTGTTTTCTTCGTTTCCGGTTCCCAGAGCGCTTCCAGCTCCTCAAGGGTTTTACCTTTGGTTTCCGGGGCAAATTTCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTa                                                                                          <  2:1187655/140‑1 (MQ=255)                                           aGATTACAGCGTAGCAGTTTGTTGTGTTTTCTTCGTTTCCGGTTCCCAGAGCGCTTCCAGCTCCTCAAGGGTTTTACCTTTGGTTTCCGGGGCAAATTTCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTaaa                                                                                        >  1:1308186/1‑140 (MQ=255)                                           aGATTACAGCGTAGCAGTTTGTTGTGTTTTCTTCGTTTCCGGTTCCCAGAGCGCTTCCAGCTCCTCAAGGGTTTTACCTTTGGTTTCCGGGGCAAATTTCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTaaa                                                                                        >  1:929137/1‑140 (MQ=255)                                             aTTACAGCGTAGCAGTTTGTTGTGTTTTCTTCGTTTCCGGTTCCCAGAGCGCTTCCAGCTCCTCAAGGGTTTTACCTTTGGTTTCCGGGGCAAATTTCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATc                                                                                      <  1:879944/140‑1 (MQ=255)                                             aTTACAGCGTAGCAGTTTGTTGTGTTTTCTTCGTTTCCGGTTCCCAGAGCGCTTCCAGCTCCTCAAGGGTTTTACCTTTGGTTTCCGGGGCAAATTTCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATc                                                                                      <  1:72024/140‑1 (MQ=255)                                              ttACAGCGTAGCAGTTTGTTGTGTTTTCTTCGTTTCCGGTTCCCAGAGCGCTTCCAGCTCCTCAAGGGTTTTACCTTTGGTTTCCGGGGCAAATTTCCACATAAACAGTGCTGCCAGAACGCCCATACa                                                                                                <  1:136975/129‑1 (MQ=255)                                              ttACAGCGTAGCAGTTTGTTGTGTTTTCTTCGTTTCCGGTTCCCAGAGCGCTTCCAGCTCCTCAAGGGTTTTACCTTTGGTTTCCGGGGCAAATTTCCACATAAACAGTGCTGCCAGAACGCCCATACa                                                                                                >  2:136975/1‑129 (MQ=255)                                                aCAGCGTAGCAGTTTGTTGTGTTTTCTTCGTTTCCGGTTCCCAGAGCGCTTCCAGCTCCTCAAGGGTTTTACCTTTGGTTTCCGGGGCAAATTTCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAg                                                                                   <  2:915144/140‑1 (MQ=255)                                                 cAGCGTAGCAGTTTGTTGTGTTTTCTTCGTTTCCGGTTCCCAGAGCGCTTCCAGCTCCTCAAGGGTTTTACCTTTGGTTTCCGGGGCAAATTTCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGt                                                                                  <  1:1255546/140‑1 (MQ=255)                                                   gCGTAGCAGTTTGTTGTGTTTTCTTCGTTTCCGGTTCCCAGAGCGCTTCCAGCTCCTCAAGGGTTTTACCTTTGGTTTCCGGGGCAAATTTCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAg                                                                                >  2:1243496/1‑140 (MQ=255)                                                    cGTAGCAGTTTGTTGTGTTTTCTTCGTTTCCGGTTCCCAGAGCGCTTCCAGCTCCTCAAGGGTTTTACCTTTGGTTTCCGGGGCAAATTTCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAgg                                                                               >  1:969370/1‑140 (MQ=255)                                                    cGTAGCAGTTTGTTGTGTTTTCTTCGTTTCCGGTTCCCAGAGCGCTTCCAGCTCCTCAAGGGTTTTACCTTTGGTTTCCGGGGCAAATTTCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAgg                                                                               >  1:212013/1‑140 (MQ=255)                                                       aGCAGTTTGTTGTGTTTTCTTCGCTTCCGGTTCCCAGAGCGCTTCCAGCTCCCCACGGGTTTTACCTTTCGTTTCCGGGGCAAATTTCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGgaga                                                                            >  2:444058/1‑140 (MQ=255)                                                         cAGTTTGTTGTGTTTTCTTCGTTTCCGGTTCCCAGAGCGCTTCCAGCTCCTCAAGGGTTTTACCTTTGGTTTCCGGGGCAAATTTCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGaaa                                                                          <  1:945295/140‑1 (MQ=255)                                                         cAGTTTGTTGTGTTTTCTTCGTTTCCGGTTCCCAGAGCGCTTCCAGCTCCTCAAGGGTTTTACCTTTGGTTTCCGGGGCAAATTTCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGaaa                                                                          <  2:969776/140‑1 (MQ=255)                                                          aGTTTGTTGTGTTTTCTTCGTTTCCGGTTCCCAGAGCGCTTCCAGCTCCTCAAGGGTTTTACCTTTGGTTTCCGGGGCAAATTTCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAAc                                                                         <  1:1336135/140‑1 (MQ=255)                                                          aGTTTGTTGTGTTTTCTTCGTTTCCGGTTCCCAGAGCGCTTCCAGCTCCTCAAGGGTTTTACCTTTGGTTTCCGGGGCAAATTTCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAAc                                                                         <  2:788276/140‑1 (MQ=255)                                                              tgttgtGTTTTCTTCGTTTCCGGTTCCCAGAGCGCTTCCAGCTCCTCAAGGGTTTTACCTTTGGTTTCCGGGGCAAATTTCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGtt                                                                     >  1:1007948/1‑140 (MQ=255)                                                              tgttgtGTTTTCTTCGTTTCCGGTTCCCAGAGCGCTTCCAGCTCCTCAAGGGTTTTACCTTTGGTTTCCGGGGCAAATTTCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGtt                                                                     <  2:1237876/140‑1 (MQ=255)                                                              tgttgtGTTTTCTTCGTTTCCGGTTCCCAGAGCGCTTCCAGCTCCTCAAGGGTTTTACCTTTGGTTTCCGGGGCAAATTTCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGtt                                                                     >  2:818324/1‑140 (MQ=255)                                                                ttgtGTTTTCTTCGTTTCCGGTTCCCAGAGCGCTTCCAGCTCCTCAAGGGTTTTACCTTTGGTTTCCGGGGCAAATTTCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTtgt                                                                   <  2:1072054/140‑1 (MQ=255)                                                                ttgtGTTTTCTTCGTTTCCGGTTCCCAGAGCGCTTCCACCTCCTCAAGGGTTTTACCTTTGGTTTCCGGGGCAAATTTCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTtgt                                                                   <  2:1183817/140‑1 (MQ=255)                                                                       ttcttcGTTTCCGGTTCCCAGAGCGCTTCCAGCTCCTCAAGGGTTTTACCTTTGGTTTCCGGGGCAAATTTCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATg                                                            >  1:362862/1‑140 (MQ=255)                                                                        tcttcGTTTCCGGTTCCCAGAGCGCTTCCAGCTCCTCAAGGGTTTTACCTTTGGTTTCCGGGGCAAATTTCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATgg                                                           >  1:1013500/1‑140 (MQ=255)                                                                            cGTTTCCGGTTCCCAGAGCGCTTCCAGCTCCTCAAGGGTTTTACCTTTGGTTTCCGGGGCAAATTTCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGcca                                                       <  1:1058644/140‑1 (MQ=255)                                                                             gTTTCCGGTTCCCAGAGCGCTTCCAGCTCCTCAAGGGTTTTACCTTTGGTTTCCGGGGCAAATTTCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGccac                                                      >  2:1095570/1‑140 (MQ=255)                                                                               ttCCGGTTCCCAGAGCGCTTCCAGCTCCTCAAGGGTTTTACCTTTGGTTTCCGGGGCAAATTTCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGccacca                                                    <  2:1021480/140‑1 (MQ=255)                                                                                tCCGGTTCCCAGAGCGCTTCCAGCTCCTCAAGGGTTTTACCTTTGGTTTCCGGGGCAAATTTCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCAccag                                                   <  2:621247/140‑1 (MQ=255)                                                                                  cGGTTCCCAGAGCGCTTCCAGCTCCTCAAGGGTTTTACCTTTGGTTTCCGGGGCAAATTTCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCAccagcc                                                 <  1:711726/140‑1 (MQ=255)                                                                                    gTTCCCAGAGCGCTTCCAGCTCCTCAAGGGTTTTACCTTTGGTTTCCGGGGCAAATTTCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCAccagccag                                               <  1:655096/140‑1 (MQ=255)                                                                                     ttCCCAGAGCGCTTCCAGCTCCTCAAGGGTTTTACCTTTGGTCTCCGGGGCAAATTTCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAgg                                              <  2:1318135/140‑1 (MQ=255)                                                                                                cTTCCAGCTCCTCAAGGGTTTTACCTTTGGTTTCCGGGGCAAATTTCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTcc                                   <  1:340368/140‑1 (MQ=255)                                                                                                      gCTCCTCAAGGGTTTTACCTTTGGTTTCCGGGGCAAATTTCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCatcatc                             >  1:607971/1‑140 (MQ=255)                                                                                                         cctcAAGGGTTTTACCTTTGGTTTCCGGGGCAAATTTCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCggg                          <  1:1243496/140‑1 (MQ=255)                                                                                                          ctcAAGGGTTTTACCTTTGGTTTCCGGGGCAAATTTCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGa                         >  2:1162631/1‑140 (MQ=255)                                                                                                          ctcAAGGGTTTTACCTTTGGTTTCCGGGGCAAATTTCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGa                         <  2:1015390/140‑1 (MQ=255)                                                                                                          ctcAAGGGTTTTACCTTTGGTTTCCGGGGCAAATTTCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGa                         >  1:792834/1‑140 (MQ=255)                                                                                                          ctcAAGGGTTTTACCTTTGGTTTCCGGGGCAAATTTCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGa                         <  2:744604/140‑1 (MQ=255)                                                                                                             aaGGGTTTTACCTTTGGTTTCCGGGGCAAATTTCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAgg                      >  2:741168/1‑140 (MQ=255)                                                                                                               gggTTTTACCTTTGGTTTCCGGGGCAAATTTCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTc                    >  1:74487/1‑140 (MQ=255)                                                                                                                 gTTTTACCTTTGGTTTCCGGGGCAAATTTCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCa                  >  2:1386881/1‑140 (MQ=255)                                                                                                                    ttACCTTTGGTTTCCGGGGCAAATTTCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGa                         >  2:850358/1‑130 (MQ=255)                                                                                                                    ttACCTTTGGTTTCCGGGGCAAATTTCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGa                         <  1:850358/130‑1 (MQ=255)                                                                                                                    ttACCTTTGGTTTCCGGGGCAAATTTCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCAGga               >  1:416967/1‑140 (MQ=255)                                                                                                                            ggTTTCCGGGGCAAATTTCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCAGGAGACGAagt       >  1:710759/1‑140 (MQ=255)                                                                                                                               ttCCGGGGCAAATTTCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCAGGAGACGAagtagt    <  1:310698/140‑1 (MQ=255)                                                                                                                               ttCCGGGGCAAATTTCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCAGGAGACGAagtagt    <  2:992389/140‑1 (MQ=255)                                                                                                                                 ccGGGGCAAATTTCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCAGGAGACGAAGTAGTTc  >  1:1389029/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                       AAAAAGTCAGCACGCCGAAATGGCGCGGCGTGCTGGACAGGAAGATTACAGCGTAGCAGTTTGTTGTGTTTTCTTCGTTTCCGGTTCCCAGAGCGCTTCCAGCTCCTCAAGGGTTTTACCTTTGGTTTCCGGGACAAATTTCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCAGGAGACGAAGTAGTTC  >  NC_000913/4240734‑4241001

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑