Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,302,315	A→G	L255P (CTG→CCG)	mdtO ←	putative multidrug efflux pump subunit MdtO

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,302,315	0	A	G	100.0%	233.7 / NA	66	L255P (CTG→CCG)	mdtO	putative multidrug efflux pump subunit MdtO
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (30/36);  total (30/36)

ACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCAGCGTCGAGTAAATGTAGGTTACCGTTGCCACGCAGCTTTGCCACCATGCGTTTTGAGTTCGCCAGTTGGCATCGTCCGCGAGGCAAAAGACATTGAGTTTTTGTAGCGCCAGCGCCTCTCTTTCAATCCGCGTTTC  >  NC_000913/4302179‑4302451                                                                                                                                         |                                                                                                                                         actGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCGGCg                                                                                                                                       <  2:572390/140‑1 (MQ=255) actGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCGGCg                                                                                                                                       <  1:433252/140‑1 (MQ=255) actGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCGGCg                                                                                                                                       <  1:1402466/140‑1 (MQ=255) actGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCGGCg                                                                                                                                       <  2:747478/140‑1 (MQ=255)  ctGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCGGCGt                                                                                                                                      <  1:490419/140‑1 (MQ=255)  ctGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCGGCGt                                                                                                                                      <  1:932924/140‑1 (MQ=255)  ctGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCGGCGt                                                                                                                                      <  2:772758/140‑1 (MQ=255)     aTCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCGGCGTCGa                                                                                                                                   <  2:1266864/140‑1 (MQ=255)      tCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCGGCGTCGAg                                                                                                                                  <  2:849927/140‑1 (MQ=255)      tCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCGGCGTCGAg                                                                                                                                  <  2:820732/140‑1 (MQ=255)       ccGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCGGCGTCGAGt                                                                                                                                 <  2:1326839/140‑1 (MQ=255)       ccGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCGGCGTCGAGt                                                                                                                                 >  2:924490/1‑140 (MQ=255)         gCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCGGCGTCGAGTaa                                                                                                                               <  1:930362/140‑1 (MQ=255)          ccAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCGGCGTCGAGTaaa                                                                                                                              >  1:1059085/1‑140 (MQ=255)          ccAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCGGCGTCGAGTaaa                                                                                                                              <  1:924490/140‑1 (MQ=255)            aGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCGGCGTCGAGTAAATg                                                                                                                            <  1:1395529/140‑1 (MQ=255)               cGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAATTTCTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCGGCGTCGAGTAAATGTAg                                                                                                                         >  2:331703/1‑140 (MQ=255)                    tGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCGGCGTCGAGTAAATGTAGGTTAc                                                                                                                    <  1:827773/140‑1 (MQ=255)                         gCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCGGCGTCGAGTAAATGTAGGTTACCGTTg                                                                                                               <  1:279587/140‑1 (MQ=255)                            cTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCGGCGTCGAGTAAATGTAGGTTACCGTTGCCa                                                                                                            <  1:917981/140‑1 (MQ=255)                               aCCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCGGCGTCGAGTAAATGTAGGTTACCGTTGCCACGc                                                                                                         >  1:734263/1‑140 (MQ=255)                                    cAGCAACGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCGGCGTCGAGTAAATGTAGGTTACCGTTGCCACGCAGCtt                                                                                                    >  2:716022/1‑140 (MQ=255)                                     aGCAACGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCGGCGTCGAGTAAATGTAGGTTACCGTTGCCACGCAGCttt                                                                                                   <  1:1394434/140‑1 (MQ=255)                                         aCGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCGGCGTCGAGTAAATGTAGGTTACCGTTGCCACGCAGCTTTGcca                                                                                               >  1:445182/1‑140 (MQ=255)                                          cGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCGGCGTCGAGTAAATGTAGGTTATCGTTGCCACGCAGCTTTGccac                                                                                              >  2:252841/1‑140 (MQ=255)                                          cGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCGGCGTCGAGTAAATGTAGGTTACCGTTGCCACGCGGCTTTGccac                                                                                              >  1:945151/1‑140 (MQ=255)                                            gCATGCTGCAGCTTGTTGATTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCGGCGTCGAGTAAATGTAGGTTACCGTTGCCACGCAGCTTTGccacca                                                                                            <  1:1219472/140‑1 (MQ=255)                                             cATGCTGCAGCTTGTTGATTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCGGCGTCGAGTAAATGTAGGTTACCGTTGCCACGCAGCTTTGCCACCAt                                                                                           <  1:870648/140‑1 (MQ=255)                                                    cAGCTTGTTGATTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCGGCGTCGAGTAAATGTAGGTTACCGTTGCCACGCAGCTTTGCCACCATGCGtttt                                                                                    >  1:848667/1‑140 (MQ=255)                                                    cAGCTTGTTGATTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCGGCGTCGAGTAAATGTAGGTTACCGTTGCCACGCAGCTTTGCCACCATGCGtttt                                                                                    >  2:401230/1‑140 (MQ=255)                                                         tgttgATTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCGGCGTCGAGTAAATGTAGGTTACCGTTGCCACGCAGCTTTGCCACCATGCGTTTTGAGtt                                                                               >  1:18818/1‑140 (MQ=255)                                                           ttgATTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCGGCGTCGAGTAAATGTAGGTTACCGTTGCCACGCAGCTTTGCCACCATGCGTTTTGAGTTCg                                                                             <  2:18818/140‑1 (MQ=255)                                                           ttgATTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCGGCGTCGAGTAAATGTAGGTTACCGTTGCCACGCAGCTTTGCCACCATGCGTTTTGAGTTCg                                                                             <  1:1388568/140‑1 (MQ=255)                                                           ttgATTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCGGCGTCGAGTAAATGTAGGTTACCGTTGCCACGCAGCTTTGCCACCATGCGTTTTGAGTTCg                                                                             <  2:924671/140‑1 (MQ=255)                                                            tgATTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCGGCGTCGAGTAAATGTAGGTTACCGTTGCCACGCAGCTTTGCCACCATGCGTTTTGAGTTCGc                                                                            >  2:533977/1‑140 (MQ=255)                                                             gATTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCGGCGTCGAGTAAATGTAGGTTACCGTTGCCACGCAGCTTTGCCACCATGCGTTTTGAGTTCGcc                                                                           <  1:655865/140‑1 (MQ=255)                                                                    gAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCGGCGTCGAGTAAATGTAGGTTACCGTTGCCACGCAGCTTTGCCACCATGCGTTTTGAGTTCGCCAGTTGGc                                                                    <  2:902178/140‑1 (MQ=255)                                                                     aaGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCGGCGTCGAGTAAATGTAGGTTACCGTTGCCACGCAGCTTTGCCACCATGCGTTTTGAGTTCGCCAGTTGGCa                                                                   >  1:530679/1‑140 (MQ=255)                                                                          aaTTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCGGCGTCGAGTAAATGTAGGTTACCGTTGCCACGCAGCTTTGCCACCATGCGTTTTGAGTTCg                                                                             >  2:1089457/1‑125 (MQ=255)                                                                          aaTTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCGGCGTCGAGTAAATGTAGGTTACCGTTGCCACGCAGCTTTGCCACCATGCGTTTTGAGTTCg                                                                             <  1:1089457/125‑1 (MQ=255)                                                                          aaTTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCGGCGTCGAGTAAATGTAGGTTACCGTTGCCACGCAGCTTTGCCACCATGCGTTTTGAGTTCGCCAGTTGGCATCGTc                                                              >  1:916167/1‑140 (MQ=255)                                                                            tttttGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCGGCGTCGAGTAAATGTAGGTTACCGTTGCCACGCAGCTTTGCCACCATGCGTTTTGAGTTCGCCAGTTGGCATCGTCcg                                                            <  1:1248105/140‑1 (MQ=255)                                                                            tttttGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCGGCGTCGAGTAAATGTAGGTTACCGTTGCCACGCAGCTTTGCCACCATGCGTTTTGAGTTCGCCAGTTGGCATCGTCcg                                                            <  2:288075/140‑1 (MQ=255)                                                                                  tCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCGGCGTCGAGTAAATGTAGGTTACCGTTGCCACGCAGCTTTGCCACCATGCGTTTTGAGTTCGCCAGTTGGCATCGTCCGCGAGGc                                                      >  2:688879/1‑140 (MQ=255)                                                                                          cAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCGGCGTCGAGTAAATGTAGGTTACCGTTGCCACGCAGCTTTGCCACCATGCGTTTTGAGTTCGCCAGTTGGCATCGTCCGCGAGGCAAAAGACa                                              <  2:916167/140‑1 (MQ=255)                                                                                                ttGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCGGCGTCGAGTAAATGTAGGTTACCGTTGCCACGCAGCTTTGCCACCATGCGTTTTGAGTTCGCCAGTTGGCATCGTCCGCGAGGCAAAAGACATTGAGt                                        >  2:742267/1‑140 (MQ=255)                                                                                                ttGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCGGCGTCGAGTAAATGTAGGTTACCGTTGCCACGCAGCTTTGCCACCATGCGTTTTGAGTTCGCCAGTTGGCATCGTCCGCGAGGCAAAAGACATTGAGt                                        >  2:560819/1‑140 (MQ=255)                                                                                                  gCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCGGCGTCGAGTAAATGTAGGTTACCGATGCCACGCAGCTTTGCCACCATGCGTTTTGAGTTCGCCAGTTGGCATCGTCCGCGAGGCAAAAGACATTGAGttt                                      >  2:964342/1‑140 (MQ=255)                                                                                                   ccTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCGGCGTCGAGTAAATGTAGGTTACCGTTACCACGCAGCTTTGCCACCATGCGTTTTGAGTTCGCCAGTTGGCATCGTCCGCGAGGCAAAAGACATTGAGtttt                                     >  1:751789/1‑140 (MQ=255)                                                                                                               cAAAAGAGGTGGGATCGTAGCGATTCGGCGTCGAGTAAATGTAGGTTACCGTTGCCACGCAGCTTTGCCACCATGCGTTTTGAGTTCGCCAGTTGGCATCGTCCGCGAGGCAAAAGACATTGAGTTTTTGTagcgccagc                         <  1:331703/140‑1 (MQ=255)                                                                                                                 aaaGAGGTGGGATCGTAGCGATTCGGCGTCGAGTAAATGTAGGTTACCGTTGCCACGCAGCTTTGCCACCATGCGTTTTGAGTTCGCCAGTTGGCATCGTCCGCGAGGCAAAAGACATTGAGTTTTTGTagcgccagcgc                       <  2:530679/140‑1 (MQ=255)                                                                                                                   agagGTGGGATCGTAGCGATTCGGCGTCGAGTAAATGTAGGTTACCGTTGCCACGCAGCTTTGCCACCATGCGTTTTGAGTTCGCCAGTTGGCATCGTCCGCGAGGCAAAAGACATTGAGTTTTTGTagcgc                             >  2:1264795/1‑132 (MQ=255)                                                                                                                   agagGTGGGATCGTAGCGATTCGGCGTCGAGTAAATGTAGGTTACCGTTGCCACGCAGCTTTGCCACCATGCGTTTTGAGTTCGCCAGTTGGCATCGTCCGCGAGGCAAAAGACATTGAGTTTTTGTagcgc                             <  1:1264795/132‑1 (MQ=255)                                                                                                                   agagGTGGGATCGTAGCGATTCGGCGTCGAGTAAATGTAGGTTACCGTTGCCACGCAGCTTTGCCACCATGCGTTTTGAGTTCGCCAGTTGGCATCGTCCGCGAGGCAAAAGACATTGAGTTTTTGTAGCGCCAGCGCct                     >  1:106220/1‑140 (MQ=255)                                                                                                                   agagGTGGGATCGTAGCGATTCGGCGTCGAGTAAATGTAGGTTACCGTTGCCACGCAGCTTTGCCACCATGCGTTTTGAGTTCGCCAGTTGGCATCGTCCGCGAGGCAAAAGACATTGAGTTTTTGTAGCGCCAGCGCct                     <  2:161201/140‑1 (MQ=255)                                                                                                                   agagGTGGGATCGTAGCGATTCGGCGTCGAGTAAATGTAGGTTACCGTTGCCACGCAGCTTTGCCACCATGCGTTTTGAGTTCGCCAGTTGGCATCGTCCGCGAGGCAAAAGACATTGAGTTTTTGTAGCGCCAGCGCct                     <  1:364112/140‑1 (MQ=255)                                                                                                                       gTGGGATCGTAGCGATTCGGCGTCGAGTAAATGTAGGTTACCGTTGCCACGCAGCTTTGCCACCATGCGTTTTGAGTTCGCCAGTTGGCATCGTCCGCGAGGCAAAAGACATTGAGTTTTTGTAGCGCCAGCGCctctct                 >  2:1217219/1‑140 (MQ=255)                                                                                                                            aTCGTAGCGATTCGGCGTCGAGTAAATGTAGGTTACCGTTGCCACGCAGCTTTGCCACCATGCGTTTTGAGTTCGCCAGTTGGCATCGTCCGCGAGGCAAAAGACATTGAGTTTTTGTAGCGCCAGCGCCTCTCTTTCaa            >  2:434723/1‑140 (MQ=255)                                                                                                                            aTCGTAGCGATTCGGCGTCGAGTAAATGTAGGTTACCGTTGCCACGCAGCTTTGCCACCATGCGTTTTGAGTTCGCCAGTTGGCATCGTCCGCGAGGCAAAAGACATTGAGTTTTTGTAGCGCCAGCGCCTCTCTTTCaa            >  1:1173354/1‑140 (MQ=255)                                                                                                                               gTAGCGATTCGGCGTCGAGTAAATGTAGGTTACCGTTGCCACGCAGCTTTGCCACCATGCGTTTTGAGTTCGCCAGTTGGCATCGTCCGCGAGGCAAAAGACATTGAGTTTTTGTAGCGCCAGCGCCTCTCTTTCAATcc         <  1:663258/140‑1 (MQ=255)                                                                                                                                tAGCGATTCGGCGTCGAGTAAATGTAGGTTACCGTTGCCACGCAGCTTTGCCACCATGCGTTTTGAGTTCGCCAGTTGGCATCGTCCGCGAGGCAAAAGACATTGAGTTTTTGTAGCGCCAGCGCCTCTCTTTCAATCcg        >  2:826440/1‑140 (MQ=255)                                                                                                                                tAGCGATTCGGCGTCGAGTAAATGTAGGTTACCGTTGCCACGCAGCTTTGCCACCATGCGTTTTGAGTTCGCCAGTTGGCATCGTCCGCGAGGCAAAAGACATTGAGTTTTTGTAGCGCCAGCGCCTCTCTTTCAATCcg        >  1:844802/1‑140 (MQ=255)                                                                                                                                  gCGATTCGGCGTCGAGTAAATGTAGGTTACCGTTGCCACGCAGCTTTGCCACCATGCGTTTTGAGTTCGCCAGTTGGCATCGTCCGCGAGGCAAAAGACATTGAGTTTTTGTAGCGCCAGCGCCTCTCTTTCAATCcgcg      >  1:568897/1‑140 (MQ=255)                                                                                                                                    gATTCGGCGTCGAGTAAATGTAGGTTACCGTTGCCACGCAGCTTTGCCACCATGCGTTTTGAGTTCGCCAGTTGGCATCGTCCGCGAGGCAAAAGACATTGAGTTTTTGTAGCGCCAGCGCCTCTCTTTCAATCCGCGtt    <  2:1059085/140‑1 (MQ=255)                                                                                                                                     aTTCGGCGTCGAGTAAATGTAGGTTACCGTTGCCACGCAGCTTTGCCACCATGCGTTTTGAGTTCGCCAGTTGGCATCGTCCGCGAGGCAAAAGACATTGAGTTTTTGTAGCGCCAGCGCCTCTCTTTCAATCCGCGttt   >  2:278638/1‑140 (MQ=255)                                                                                                                                      ttCGGCGTCGAGTAAATGTAGGTTACCGTTGCCACGCAGCTTTGCCACCATGCGTTTTGAGTTCGCCAGTTGGCATCGTCCGCGAGGCAAAAGACATTGAGTTTTTGTAGCGCCAGCGCCTCTCTTTCAATCCGCGTTTc  >  2:943159/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                         ACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCAGCGTCGAGTAAATGTAGGTTACCGTTGCCACGCAGCTTTGCCACCATGCGTTTTGAGTTCGCCAGTTGGCATCGTCCGCGAGGCAAAAGACATTGAGTTTTTGTAGCGCCAGCGCCTCTCTTTCAATCCGCGTTTC  >  NC_000913/4302179‑4302451

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑