Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,321,418	T→C	E93E (GAA→GAG)	phnG ←	carbon‑phosphorus lyase core complex subunit PhnG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,321,418	0	T	C	100.0%	227.4 / NA	66	E93E (GAA→GAG)	phnG	carbon‑phosphorus lyase core complex subunit PhnG
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (31/35);  total (31/35)

ACCCGGCTGGCGTTCACTTCGGCCTGGCGTGCGGCAATGCGTGCCATACGGTCAGCGTCCAGCGGGGCAATAAGGGTTTCTGATAAGTTTTGAAAGTGGCGAGATTGCTGCATCAGCGCGTCAATCAGCGCGCAGCGTTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGGCGCGGGTCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGCCGGTGCCGCCCATCCGCGCCTGAATC  >  NC_000913/4321281‑4321553                                                                                                                                          |                                                                                                                                        aCCCGGCTGGCGTTCACTTCGGCCTGGCGTGCGGCAATGCGTGCCATACGGTCAGCGTCCAGCGGGGCAATAAGGGTTTCTGATAAGTTTTGAAAGTGGCGAGATTGCTGCATCAGCGCGTCAATCAGCGCGCAGCGCTc                                                                                                                                       >  2:1062426/1‑140 (MQ=255)     ggCTGGCGTTCACTTCGGCCTGGCGTGCGGCAATGCGTGCCATACGGTCAGCGTCCAGCGGGGCAATAAGGGTTTCTGATAAGTTTTGAAAGTGGCGAGATTGCTGCATCAGCGCGTCAATCAGCGCGCAGCGCTCGGCg                                                                                                                                   <  2:599729/140‑1 (MQ=255)       cTGGCGTTCACTTCGGCCTGGCGTGCGGCAATGCGTGCCATACGGTCAGCGTCCAGCGGGGCAATAAGGGTTTCTGATAAGTTTTGAAAGTGGCGAGATTGCTGCATCAGCGCGTCAATCAGCGCGCAGCGCTCGGCGTg                                                                                                                                 <  2:344168/140‑1 (MQ=255)       cTGGCGTTCACTTCGGCCTGGCGTGCGGCAATGCGTGCCATACGGTCAGCGTCCAGCGGGGCAATAAGGGTTTCTGATAAGTTTTGAAAGTGGCGAGATTGCTGCATCAGCGCGTCAATCAGCGCGCAGCGCTCGGCGTg                                                                                                                                 <  2:256850/140‑1 (MQ=255)         ggCGTTCACTTCGGCCTGGCGTGCGGCAATGCGTGCCATACGGTCAGCGTCCAGCGGGGCAATAAGGGTTTCTGATAAGTGTTGAAAGTGGCGAGATTGCTGCATCAGCGCGTCAATCAGCGCGCAGCGCTCGGCGTGCt                                                                                                                               <  2:396765/140‑1 (MQ=255)           cGTTCACTTCGGCCTGGCGTGCGGCAATGCGTGCCATACGGTCAGCGTCCAGCGGGGCAATAAGGGTTTCTGATAAGTTTTGAAAGTGGCGAGATTGCTGCATCAGCGCGTCAATCAGCGCGCAGCGCTCGGCGTGCTGt                                                                                                                             >  2:1238642/1‑140 (MQ=255)                 cTTCGGCCTGGCGTGCGGCAATGCGTGCCATACGGTCAGCGTCCAGCGGGGCAATAAGGGTTTCTGATAAGTTTTGAAAGTGGCGAGATTGCTGCATCAGCGCGTCAATCAGCGCGCAGCGCTCGGCGTGCTGTTTATCa                                                                                                                       <  2:1123201/140‑1 (MQ=255)                 cTTCGGCCTGGCGTGCGGCAATGCGTGCCATACGGTCAGCGTCCAGCGGGGCAATAAGGGTTTCTGATAAGTTTTGAAAGTGGCGAGATTGCTGCATCAGCGCGTCAATCAGCGCGCAGCGCTCGGCGTGCTGTTTATCa                                                                                                                       <  2:1336508/140‑1 (MQ=255)                 cTTCGGCCTGGCGTGCGGCAATGCGTGCCATACGGTCAGCGTCCAGCGGGGCAATAAGGGTTTCTGATAAGTTTTGAAAGTGGCGAGATTGCTGCATCAGCGCGTCAATCAGCGCGCAGCGCTCGGCGTGCTGTTTATCa                                                                                                                       <  1:1354070/140‑1 (MQ=255)                        cTGGCGTGCGGCAATGCGTGCCATACGGTCAGCGTCCAGCGGGGCAATAAGGGTTTCTGATAAGTTTTGAAAGTGGCGAGATTGCTGCATCAGCGCGTCAATCAGCGCGCAGCGCTCGGCGTGCTGTTTATCACGCCCCt                                                                                                                <  1:430418/140‑1 (MQ=255)                            cGTGCGGCAATGCGTGCCATACGGTCAGCGTCCAGCGGGGCAATAAGGGTTTCTGATAAGTTTTGAAAGTGGCGAGATTGCTGCATCAGCGCGTCAATCAGCGCGCAGCGCTCGGCGTGCTGTTTATCACGCCCCTGCAc                                                                                                            <  1:1086892/140‑1 (MQ=255)                             gTGCGGCAATGCGTGCCATACGGTCAGCGTCCAGCGGGGCAATAAGGGTTTCTGATAAGTTTTGAAAGTGGCGAGATTGCTGCATCAGCGCGTCAATCAGCGCGCAGCGCTCGGCGTGCTGTTTATCACGCCCCTGCAcc                                                                                                           <  2:844103/140‑1 (MQ=255)                                cGGCAATGCGTGCCATACGGTCAGCGTCCAGCGGGGCAATAAGGGTTTCTGATAAGTTTTGAAAGTGGCGAGATTGCTGCATCAGCGCGTCAATCAGCGCGCAGCGCTCGGCGTGCTGTTTATCACGCCCCTGCACCCAg                                                                                                        >  2:310251/1‑140 (MQ=255)                                cGGCAATGCGTGCCATACGGTCAGCGTCCAGCGGGGCAATAAGGGTTTCTGATAAGTTTTGAAAGTGGCGAGATTGCTGCATCAGCGCGTCAATCAGCGCGCAGCGCTCGGCGTGCTGTTTATCACGCCCCTGCACCCAg                                                                                                        <  2:987109/140‑1 (MQ=255)                                  gCAATGCGTGCCATACGGTCAGCGTCCAGCGGGGCAATAAGGGTTTCTGATAAGTTTTGAAAGTGGCGAGATTGCTGCATCAGCGCGTCAATCAGCGCGCAGCGCTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCt                                                                                                      <  1:1263197/140‑1 (MQ=255)                                         gTGCCATACGGTCAGCGTCCAGCGGGGCAATAAGGGTTTCTGATAAGTTTTGAAAGTGGCGAGATTGCTGCATCAGCGCGTCAATCAGCGCGCAGCGCTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCg                                                                                               <  1:329981/140‑1 (MQ=255)                                           gCCATACGGTCAGCGTCCAGCGGGGCAATAAGGGTTTCTGATAAGTTTTGAAAGTGGCGAGATTGCTGCATCAGCGCGTCAATCAGCGCGCAGCGCTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAg                                                                                             >  1:1145617/1‑140 (MQ=255)                                            ccATACGGTCAGCGTCCAGCGGGGCAATAAGGGTTTCTGATAAGTTTTGAAAGTGGCGAGATTGCTGCATCAGCGCGTCAATCAGCGCGCAGCGCTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCt                                                                                                      <  2:1350320/130‑1 (MQ=255)                                            ccATACGGTCAGCGTCCAGCGGGGCAATAAGGGTTTCTGATAAGTTTTGAAAGTGGCGAGATTGCTGCATCAGCGCGTCAATCAGCGCGCAGCGCTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCt                                                                                                      >  1:1350320/1‑130 (MQ=255)                                             cATACGGTCAGCGTCCAGCGGGGCAATAAGGGTTTCTGATAAGTTTTGAAAGTGGCGAGATTGCTGCATCAGCGCGTCAATCAGCGCGCAGCGCTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCg                                                                                           <  2:685483/140‑1 (MQ=255)                                              aTACGGTCAGCGTCCAGCGGGGCAATAAGGGTTTCTGATAAGTTTTGAAAGTGGCGAGATTGCTGCATCAGCGCGTCAATCAGCGCGCAGCGCTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGt                                                                                          <  1:310251/140‑1 (MQ=255)                                                        cGTCCAGCGGGGCAATAAGGGTTTCTGATAAGTTTTGAAAGTGGCGAGATTGCTGCATCAGCGCGTCAATCAGCGCGCAGCGCTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCgtcagtc                                                                                <  2:809809/140‑1 (MQ=255)                                                             aGCGGGGCAATAAGGGTTTCTGATAAGTTTTGAAAGTGGCGAGATTGCTGCATCAGCGCGTCAATCAGCGCGCAGCGCTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGgcg                                                                           <  2:984497/140‑1 (MQ=255)                                                                  ggCAATAAGGGTTTCTGATAAGTTTTGAAAGTGGCGAGATTGCTGCATCAGCGCGTCAATCAGCGCGCAGCGCTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCAcgg                                                                      >  2:1035327/1‑140 (MQ=255)                                                                  ggCAATAAGGGTTTCTGATAAGTTTTGAAAGTGGCGAGATTGCTGCATCAGCGCGTCAATCAGCGCGCAGCGCTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCAcgg                                                                      >  1:871453/1‑140 (MQ=255)                                                                  ggCAATAAGGGTTTCTGATAAGTTTTGAAAGTGGCGAGATTGCTGCATCAGCGCGTCAATCAGCGCGCAGCGCTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCAcgg                                                                      >  2:879388/1‑140 (MQ=255)                                                                       tAAGGGTTTCTGATAAGTTTTGAAAGTGGCGAGATTGCTGCATCAGCGCGTCAATCAGCGCGCAGCGCTCGGCGTGCTGTTTATCACGCCCCTGCACGCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGgcggcg                                                                 >  2:1317239/1‑140 (MQ=255)                                                                       tAAGGGTTTCTGATAAGTTTTGAAAGTGGCGAGATTGCTGCATCAGCGCGTCAATCAGCGCGCAGCGCTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGgcggcg                                                                 <  2:57935/140‑1 (MQ=255)                                                                       tAAGGGTTTCTGATAAGTTTTGAAAGTGGCGAGATTGCTGCATCAGCGCGTCAATCAGCGCGCAGCGCTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGgcggcg                                                                 <  2:556078/140‑1 (MQ=255)                                                                         aGGGTTTCTGATAAGTTTTGAAAGTGGCGAGATTGCTGCATCAGCGCGTCAATCAGCGCGCAGCGCTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGGcgcg                                                               >  1:486756/1‑140 (MQ=255)                                                                            gTTTCTGATAAGTTTTGAAAGTGGCGAGATTGCTGCATCAGCGCGTCAATCAGCGCGCAGCGCTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGGCGCGGGt                                                            >  1:352514/1‑140 (MQ=255)                                                                            gTTTCTGATAAGTTTTGAAAGTGGCGAGATTGCTGCATCAGCGCGTCAATCAGCGCGCAGCGCTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGGCGCGGGt                                                            <  2:283211/140‑1 (MQ=255)                                                                            gTTTCTGATAAGTTTTGAAAGTGGCGAGATTGCTGCATCAGCGCGTCAATCAGCGCGCAGCGCTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGGCGCGGGt                                                            <  1:332393/140‑1 (MQ=255)                                                                               tCTGATAAGTTTTGAAAGTGGCGAGATTGCTGCATCAGCGCGTCAATCAGCGCGCAGCGCTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGGCGCGGGTCAg                                                         >  1:1246831/1‑140 (MQ=255)                                                                                 tGATAAGTTTTGAAAGTGGCGAGATTGCTGCATCAGCGCGTCAATCAGCGCGCAGCGCTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGGCGCGGGTCAGCg                                                       >  2:1018339/1‑140 (MQ=255)                                                                                     aaGTTTTGAAAGTGGCGAGATTGCTGCATCAGCGCGTCAATCAGCGCGCAGCGCTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGGCGCGGGTCAGCGTGGc                                                   >  1:425969/1‑140 (MQ=255)                                                                                      aGTTTTGAAAGTGGCGAGATTGCTGCATCAGCGCGTCAATCAGCGCGCAGCGCTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGAAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGGCGCGGGTCAGCGTGGCg                                                  >  1:561470/1‑140 (MQ=255)                                                                                          ttGAAAGTGGCGAGATTGCTGCATCAGCGCGTCAATCAGCGCGCAGCGCTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGGCGCGGGTCAGCGTGGCGTCGc                                              <  2:887629/140‑1 (MQ=255)                                                                                                  ggCGAGATTGCTGCATCAGCGCGTCAATCAGCGCGCAGCGCTCGGCGTGCTGTTTTTCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGGCGCGGGTCAGCGTGGCGTCGCCGGCaaaa                                      >  1:417135/1‑140 (MQ=255)                                                                                                  ggCGAGATTGCTGCATCAGCGCGTCAATCAGCGCGCAGCGCTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGGCGCGGGTCAGCGTGGCGTCGCCGGCaaaa                                      >  2:790368/1‑140 (MQ=255)                                                                                                       gaTTGCTGCATCAGCGCGTCAATCAGCGCGCAGCGCTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGGCGCGGGTCAGCGTGGCGTCGCCGGCAAAAAAACg                                 <  1:410613/140‑1 (MQ=255)                                                                                                             tgcATCAGCGCGTCAATCAGCGCGCAGCGCTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGGCGCGGGTCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGcc                           >  2:67048/1‑140 (MQ=255)                                                                                                               cATCAGCGCGTCAATCAGCGCGCAGCGCTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGGCGCGGGTCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGCCgg                         <  1:186479/140‑1 (MQ=255)                                                                                                                 tCAGCGCGTCAATCAGCGCGCAGCGCTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGGCTCGGGTCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGc                            >  1:310436/1‑135 (MQ=255)                                                                                                                 tCAGCGCGTCAATCAGCGCGCAGCGCTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGGCTCGGGTCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGc                            <  2:310436/135‑1 (MQ=255)                                                                                                                 tCAGCGCGTCAATCAGCGCGCAGCGCTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGGCGCGGGTCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGc                            <  1:1199908/135‑1 (MQ=255)                                                                                                                 tCAGCGCGTCAATCAGCGCGCAGCGCTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGGCGCGGGTCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGc                            >  2:1199908/1‑135 (MQ=255)                                                                                                                 tCAGCGCGTCAATCAGCGCGCAGCGCTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGGCGCGGGTCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGCCGGTg                       >  1:151137/1‑140 (MQ=255)                                                                                                                 tCAGCGCGTCAATCAGCGCGCAGCGCTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGGCGCGGGTCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGCCGGTg                       >  1:898681/1‑140 (MQ=255)                                                                                                                       cgTCAATCAGCGCGCAGCGCTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGGCGCGGGTCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGCCGGTGCCGccc                 >  2:44850/1‑140 (MQ=255)                                                                                                                         tCAATCAGCGCGCAGCGCTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGGCGCGGGTCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGCCGGTGCCGCCCAt               >  2:912963/1‑140 (MQ=255)                                                                                                                          cAATCAGCGCGCAGCGCTCGGCGTGCTGTTTATCACGCCCCTGCACCC‑GCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGGCGCGGGTCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGCCGGTGCCGCCCATcc             <  2:1057140/140‑1 (MQ=255)                                                                                                                           aaTCAGCGCGCAGCGCTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGGCGCGGGTCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGCCGGTGCCGCCCATcc             <  2:425969/140‑1 (MQ=255)                                                                                                                           aaTCAGCGCGCAGCGCTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGGCGCGGGTCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGCCGGTGCCGCCCATcc             >  1:822769/1‑140 (MQ=255)                                                                                                                               aGCGCGCAGCGCTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGGCGCGGGTCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGCCGGTGCCGCCCATCCgcgc         >  2:1030886/1‑140 (MQ=255)                                                                                                                               aGCGCGCAGCGCTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGGCGCGGGTCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGCCGGTGCCGCCCATCCgcgc         >  2:719023/1‑140 (MQ=255)                                                                                                                                gcgcgcAGCGCTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGGCGCGGGTCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGCCGGTGCCGCCCATCCGCGcc        <  2:422388/140‑1 (MQ=255)                                                                                                                                gcgcgcAGCGCTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGGCGCGGGTCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGCCGGTGCCGCCCATCCGCGcc        <  1:871520/140‑1 (MQ=255)                                                                                                                                 cgcgcAGCGCTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGGCGCGGGTCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGCCGGTGCCGCCCATCCGCGCCt       <  2:822769/140‑1 (MQ=255)                                                                                                                                 cgcgcAGCGCTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGGCGCGGGTCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGCCGGTGCCGCCCATCCGCGCCt       <  2:486756/140‑1 (MQ=255)                                                                                                                                 cgcgcAGCGCTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGGCGCGGGTCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGCCGGTGCCGCCCATCCGCGCCt       <  1:815364/140‑1 (MQ=255)                                                                                                                                 cgcgcAGCGCTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGGCGCGGGTCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGCCGGTGCCGCCCATCCGCGCCt       >  2:1209571/1‑140 (MQ=255)                                                                                                                                  gcgcAGCGCTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGGCGCGGGTCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGCCGGTGCCGCCCATCCGCGCCTg      >  1:1378982/1‑140 (MQ=255)                                                                                                                                   cgcAGCGCTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGGCGCGGGTCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGCCGGTGCCGCCCATCCGCGCCTGa     >  1:906531/1‑140 (MQ=255)                                                                                                                                     cAGCGCTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGGCGCGGGTCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGCCGGTGCCGCCCATCCGCGCCTGAAt   <  2:1145617/140‑1 (MQ=255)                                                                                                                                      aGCGCTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGGCGCGGGTCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGCCGGTGCCGCCCATCCGCGCCTGAATc  >  2:1157328/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                        ACCCGGCTGGCGTTCACTTCGGCCTGGCGTGCGGCAATGCGTGCCATACGGTCAGCGTCCAGCGGGGCAATAAGGGTTTCTGATAAGTTTTGAAAGTGGCGAGATTGCTGCATCAGCGCGTCAATCAGCGCGCAGCGTTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGGCGCGGGTCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGCCGGTGCCGCCCATCCGCGCCTGAATC  >  NC_000913/4321281‑4321553

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑