Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,510,298	A→G	intergenic (+165/+392)	yjhV → / ← fecE	KpLE2 phage‑like element; putative uncharacterized protein YjhV/ferric citrate ABC transporter ATP binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,510,298	0	A	G	100.0%	232.1 / NA	66	intergenic (+165/+392)	yjhV/fecE	KpLE2 phage‑like element; putative uncharacterized protein YjhV/ferric citrate ABC transporter ATP binding subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (32/34);  total (32/34)

CCTACCTAAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGTTGCCGT  >  NC_000913/4510163‑4510433                                                                                                                                        |                                                                                                                                        cctacctaAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCGTTgg                                                                                                                                     <  1:776348/140‑1 (MQ=255)    acctaAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCGTTGGTaa                                                                                                                                  <  2:575274/140‑1 (MQ=255)    acctaAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCGTTGGTaa                                                                                                                                  >  2:94606/1‑140 (MQ=255)         agtagtAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCGTTGGTAATGAAc                                                                                                                             <  2:761143/140‑1 (MQ=255)            agtagCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCGTTGGTAATGAACCAg                                                                                                                          <  2:1296724/140‑1 (MQ=255)              tagCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCGTTGGTAATGAACCAGCt                                                                                                                        >  2:460913/1‑140 (MQ=255)              tagCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCGTTGGTAATGAACCAGCt                                                                                                                        >  2:1123596/1‑140 (MQ=255)               agCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCGTTGGTAATGAACCAGCTc                                                                                                                       >  2:155053/1‑140 (MQ=255)                gCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCGTTGGTAATGAACCAGCTcc                                                                                                                      >  1:491259/1‑140 (MQ=255)                gCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCGTTGGTAATGAACCAGCTcc                                                                                                                      >  2:274294/1‑140 (MQ=255)                  aaaCGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCGTTGGTAATGAACCAGCTCCGt                                                                                                                    <  1:670926/140‑1 (MQ=255)                   aaCGCACACTTTTTGGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCGTTGGTAATGAACCAGCTCCGTg                                                                                                                   >  1:1045150/1‑140 (MQ=255)                   aaCGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCGTTGGTAATGAACCAGCTCCGTg                                                                                                                   >  1:319230/1‑140 (MQ=255)                   aaCGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCGTTGGTAATGAACCAGCTCCGTg                                                                                                                   <  1:326979/140‑1 (MQ=255)                     cGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGTTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCGTTGGTAATGAACCAGCTCCGTGaa                                                                                                                 >  2:837914/1‑140 (MQ=255)                     cGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCGTTGGTAATGAACCAGCTCCGTGaa                                                                                                                 >  2:836466/1‑140 (MQ=255)                      gCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCGTTGGTAATGAACCAGCTCCGTGAAt                                                                                                                <  2:489504/140‑1 (MQ=255)                         cacTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCGTTGGTAATGAACCAGCTCCGTGAATGTc                                                                                                             <  2:319230/140‑1 (MQ=255)                         cacTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCGTTGGTAATGAACCAGCTCCGTGAATGTc                                                                                                             <  2:1075778/140‑1 (MQ=255)                         cacTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCGTTGGTAATGAACCAGCTCCGTGAATGTc                                                                                                             >  2:1055336/1‑140 (MQ=255)                         cacTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCGTTGGTAATGAACCAGCTCCGTGAATGTc                                                                                                             <  2:491259/140‑1 (MQ=255)                         cacTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCGTTGGTAATGAACCAGCTCCGTGAATGTc                                                                                                             <  2:61462/140‑1 (MQ=255)                         cacTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCGTTGGTAATGAACCAGCTCCGTGAATGTc                                                                                                             <  2:694425/140‑1 (MQ=255)                         cacTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCGTTGGTAATGAACCAGCTCCGTGAATGTc                                                                                                             <  1:1206920/140‑1 (MQ=255)                         cacTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCGTTGGTAATGAACCAGCTCCGTGAATGTc                                                                                                             <  1:1162145/140‑1 (MQ=255)                         cacTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCGTTGGTAATGAACCAGCTCCGTGAATGTc                                                                                                             <  2:117367/140‑1 (MQ=255)                                            tGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCGTTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGaa                                                                                          <  1:94606/140‑1 (MQ=255)                                                         gATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCGTTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGAtctc                                                                             >  1:219925/1‑140 (MQ=255)                                                             aGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCGTTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTccccc                                                                         >  2:1167301/1‑140 (MQ=255)                                                             aGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCGTTGATAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTccccc                                                                         >  1:585131/1‑140 (MQ=255)                                                               cAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCGTTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCt                                                                       <  1:836466/140‑1 (MQ=255)                                                               cAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAACCCCACCCTCGTTGGTAATGACCCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCAAAATTTTTTGATCCCCCCCCt                                                                       <  2:961772/140‑1 (MQ=255)                                                                 aTTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCGTTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGa                                                                     <  2:102454/140‑1 (MQ=255)                                                                    ccGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCGTTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAAt                                                                  <  2:1052010/140‑1 (MQ=255)                                                                    ccGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCGTTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAAt                                                                  <  2:582211/140‑1 (MQ=255)                                                                        tCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCGTTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTaa                                                              >  2:789432/1‑140 (MQ=255)                                                                         ccATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCGTTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTaaa                                                             <  1:881168/140‑1 (MQ=255)                                                                             ggTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCGTTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTa                                                         <  1:331595/140‑1 (MQ=255)                                                                                     ttGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCGTTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTc                                                 <  2:1045150/140‑1 (MQ=255)                                                                                        aTTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCGTTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACg                                              >  2:1067953/1‑140 (MQ=255)                                                                                        aTTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCGTTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACg                                              >  1:1117203/1‑140 (MQ=255)                                                                                             cGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCGTTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGttttt                                         >  1:318940/1‑140 (MQ=255)                                                                                                 ggCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCGTTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGt                                     >  1:1352774/1‑140 (MQ=255)                                                                                                   cGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCGTTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGttt                                   >  2:1087322/1‑140 (MQ=255)                                                                                                   cGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCGTTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGttt                                   >  2:1261296/1‑140 (MQ=255)                                                                                                     tCTGATCATTGATTTATCCTCAAAAGCCCAACCTCGTTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCt                                 <  2:1352774/140‑1 (MQ=255)                                                                                                       tGATCATTGATTTATCCTCAAAAGCCCAACCTCGTTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTaa                               >  2:547185/1‑140 (MQ=255)                                                                                                       tGATCATTGATTTATCCTCAAAAGCCCAACCTCGTTGGTAATGAACCAGCTCCGTGAATGTCCACTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTaa                               <  1:822586/140‑1 (MQ=255)                                                                                                                   tATCCTCAAAAGCCCAACCTCGTTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTc                   >  2:734649/1‑140 (MQ=255)                                                                                                                     tCCTCAAAAGCCCAACCTCGTTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTcgc                 >  2:227526/1‑140 (MQ=255)                                                                                                                     tCCTCAAAAGCCCAACCTCGTTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTcgc                 >  1:112187/1‑140 (MQ=255)                                                                                                                          aaaaGCCCAACCTCGTTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGtttt            >  1:627228/1‑140 (MQ=255)                                                                                                                          aaaaGCCCAACCTCGTTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGtttt            >  1:524125/1‑140 (MQ=255)                                                                                                                          aaaaGCCCAACCTCGTTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGtttt            >  2:88074/1‑140 (MQ=255)                                                                                                                             aGCCCAACCTCGTTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCg         <  1:1067953/140‑1 (MQ=255)                                                                                                                              gCCCAACCTCGTTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGt        <  2:944391/140‑1 (MQ=255)                                                                                                                              gCCCAACCTCGTTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGt        <  1:14573/140‑1 (MQ=255)                                                                                                                               cccAACCTCGTTGGTAATGAACCATCTCCGTGAATGTCGGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGtt       <  2:1324748/140‑1 (MQ=255)                                                                                                                               cccAACCTCGTTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGtt       >  1:374781/1‑140 (MQ=255)                                                                                                                               cccAACCTCGTTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGtt       <  1:1184762/140‑1 (MQ=255)                                                                                                                               cccAACCTCGTTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGtt       >  1:1343462/1‑140 (MQ=255)                                                                                                                               cccAACCTCGTTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGtt       >  1:476728/1‑140 (MQ=255)                                                                                                                                    ccTCGTTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGTTGCCGt  <  1:1167301/140‑1 (MQ=255)                                                                                                                                    ccTCGTTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGTTGCCGt  >  2:610965/1‑140 (MQ=255)                                                                                                                                    ccTCGTTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGTTGCCGt  <  1:610965/140‑1 (MQ=255)                                                                                                                                    ccTCGTTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGTTGCCGt  <  2:219925/140‑1 (MQ=255)                                                                                                                                    ccTCGTTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGTTGCCGt  <  2:1117203/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                        CCTACCTAAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGTTGCCGT  >  NC_000913/4510163‑4510433

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑