Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,599,629	A→G	intergenic (‑188/+66)	opgB ← / ← yjjA	phosphoglycerol transferase I/DUF2501 domain‑containing protein YjjA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,599,629	0	A	G	100.0%	292.9 / NA	83	intergenic (‑188/+66)	opgB/yjjA	phosphoglycerol transferase I/DUF2501 domain‑containing protein YjjA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (44/39);  total (44/39)

GAAGCACAATGTAATGGCGTCGCCATATTGCTGCAATTCTAGTGTCAAAAAAATGCGATGTTGTTAGGAATTAGTTTATAAACAGACTTTTTTGATTACGGTCACTTCCCGTGTAACAGGGGTAAAACCGTCCAGGAGCAGGGAAAAGGGCAGGAGAGAGCCGCGTCAGCCGGGTAACAGCTAAAACGCGGCGTTTCGCGGTTCAGGAAATGAAGTTCAGCCCCTGTTTTAACACCAGATCGCAGGCTTTGGTTTTCACCTTTTCTGCCAG  >  NC_000913/4599493‑4599763                                                                                                                                         |                                                                                                                                       gaAGCACAATGTAATGGCGTCGCCATATTGCTGCAATTCTAGTGTCAAAAAAATGCGATGTTGTTAGGAATTAGTTTATAAACAGACTTTTTTGATTACGGTCACTTCCCGTGTAACAGGGGTAAAACCGTCCAGGGGCa                                                                                                                                     <  2:360686/140‑1 (MQ=255)    gCACAATGTAATGGCGTCGCCATATTGCTGCAATTCTAGTGTCAAAAAAATGCGATGTTGTTAGGAATTAGTTTATAAACAGACTTTTTTGATTACGGTCACTTCCCGTGTAACAGGGGTAAAACCGTCCAGGGGCAggg                                                                                                                                  >  1:1145766/1‑140 (MQ=255)    gCACAATGTAATGGCGTCGCCATATTGCTGCAATTCTAGTGTCAAAAAAATGCGATGTTGTTAGGAATTAGTTTATAAACAGACTTTTTTGATTACGGTCACTTCCCGTGTAACAGGGGTAAAACCGTCCAGGGGCAggg                                                                                                                                  >  1:809951/1‑140 (MQ=255)     cacaATGTAATGGCGTCGCCATATTGCTGCAATTCTAGTGTCAAAAAAATGCGATGTTGTTAGGAATTAGTTTATAAACAGACTTTTTTGATTACGGTCACTTCCCGTGTAACAGGGGTAAAACCGTCCAGGGGCAGGGa                                                                                                                                 >  2:1330468/1‑140 (MQ=255)     cacaATGTAATGGCGTCGCCATATTGCTGCAATTCTAGTGTCAAAAAAATGCGATGTTGTTAGGAATTAGTTTATAAACAGACTTTTTTGATTACGGTCACTTCCCGTGTAACAGGGGTAAAACCGTCCAGGGGCAGGGa                                                                                                                                 >  2:428140/1‑140 (MQ=255)      acaATGTAATGGCGTCGCCATATTGCTGCAATTCTAGTGTCAAAAAAATGCGATGTTGTTAGGAATTAGTTTATAAACAGTCTTTTTCGATTACGGTCACTTCCCGTGTAACAGGGGTAAAACCGTCCAGGGGCAGGGaa                                                                                                                                <  2:717648/140‑1 (MQ=255)      acaATGTAATGGCGTCGCCATATTGCTGCAATTCTAGTGTCAAAAAAATGCGATGTTGTTAGGAATTAGTTTATAAACAGACTTTTTTGATTACGGTCACTTCCCGTGTAACAGGGGTAAAACCGTCCAGGGGCAGGGaa                                                                                                                                <  1:1098054/140‑1 (MQ=255)      acaATGTAATGGCGTCGCCATATTGCTGCAATTCTAGTGTCAAAAAAATGCGATGTTGTTAGGAATTAGTTTATAAACAGACTTTTTTGATTACGGTCACTTCCCGTGTAACAGGGGTAAAACCGTCCAGGGGCAGGGaa                                                                                                                                <  1:856093/140‑1 (MQ=255)      acaATGTAATGGCGTCGCCATATTGCTGCAATTCTAGTGTCAAAAAAATGCGATGTTGTTAGGAATTAGTTTATAAACAGACTTTTTTGATTACGGTCACTTCCCGTGTAACAGGGGTAAAACCGTCCAGGGGCAGGGaa                                                                                                                                <  1:268065/140‑1 (MQ=255)           gTAATGGCGTCGCCATATTGCTGCAATTCTAGTGTCAAAAAAATGCGATGTTGTTAGGAATTAGTTTATAAACAGACTTTTTTGATTACGGTCACTTCCCGTGTAACAGGGGTAAAACCGTCCAGGGGCAGGGAAAAggg                                                                                                                           <  1:1262139/140‑1 (MQ=255)           gTAATGGCGTCGCCATATTGCTGCAATTCTAGTGTCAAAAAAATGCGATGTTGTTAGGAATTAGTTTATAAACAGACTTTTTTGATTACGGTCACTTCCCGTGTAACAGGGGTAAAACCGTCCAGGGGCAGGGAAAAggg                                                                                                                           <  1:1355776/140‑1 (MQ=255)               tGGCGTCGCCATATTGCTGCAATTCTAGTGTCAAAAAAATGCGATGTTGTTAGGAATTAGTTTATAAACAGACTTTTTTGATTACGGTCACTTCCCGTGTAACAGGGGTAAAACCGTCCAGGGGCAGGGaaa                                                                                                                               <  2:131571/132‑1 (MQ=255)               tGGCGTCGCCATATTGCTGCAATTCTAGTGTCAAAAAAATGCGATGTTGTTAGGAATTAGTTTATAAACAGACTTTTTTGATTACGGTCACTTCCCGTGTAACAGGGGTAAAACCGTCCAGGGGCAGGGaaa                                                                                                                               >  1:131571/1‑132 (MQ=255)               tGGCGTCGCCATATTGCTGCAATTCTAGTGTCAAAAAAATGCGATGTTGTTAGGAATTAGTTTATAAACAGACTTTTTTGATTACGGTCACTTCCCGTGTAACAGGGGTAAAACCGTCCAGGGGCAGGGAAAAGGGCAgg                                                                                                                       >  2:12116/1‑140 (MQ=255)               tGGCGTCGCCATATTGCTGCAATTCTAGTGTCAAAAAAATGCGATGTTGTTAGGAATTAGTTTATAAACAGACTTTTTTGATTACGGTCACTTCCCGTGTAACAGGGGTAAAACCGTCCAGGGGCAGGGAAAAGGGCAgg                                                                                                                       >  2:1340531/1‑140 (MQ=255)                  cGTCGCCATATTGCTGCAATTCTAGTGTCAAAAAAATGCGATGTTGTTAGGAATTAGTTTATAAACAGACTTTTTTGATTACGGTCACTTCCCGTGTAACAGGGGTAAAACCGTCCAGGGGCAGGGaa                                                                                                                                >  2:1202772/1‑128 (MQ=255)                  cGTCGCCATATTGCTGCAATTCTAGTGTCAAAAAAATGCGATGTTGTTAGGAATTAGTTTATAAACAGACTTTTTTGATTACGGTCACTTCCCGTGTAACAGGGGTAAAACCGTCCAGGGGCAGGGaa                                                                                                                                <  1:1202772/128‑1 (MQ=255)                  cGTCGCCATATTGCTGCAATTCTAGTGTCAAAAAAATGCGATGTTGTTAGGAATTAGTTTATAAACAGACTTTTTTGATTACGGTCACTTCCCGTGTAACAGGGGTAAAACCGTCCAGGGGCAGGGAAAAGGGCAGgaga                                                                                                                    >  2:1188057/1‑140 (MQ=255)                  cGTCGCCATATTGCTGCAATTCTAGTGTCAAAAAAATGCGATGTTGTTAGGAATTAGTTTATAAACAGACTTTTTTGATTACGGTCACTTCCCGTGTAACAGGGGTAAAACCGTCCAGGGGCAGGGAAAAGGGCAGgaga                                                                                                                    >  2:1294625/1‑140 (MQ=255)                  cGTCGCCATATTGCTGCAATTCTAGTGTCAAAAAAATGCGATGTTGTTAGGAATTAGTTTATAAACAGACTTTTTTGATTACGGTCACTTCCCGTGTAACAGGGGTAAAACCGTCCAGGGGCAGGGAAAAGGGCAGgaga                                                                                                                    >  1:152237/1‑140 (MQ=255)                    tCGCCATATTGCTGCAATTCTAGTGTCAAAAAAATGCGATGTTGTTAGGAATTAGTTTATAAACAGACTTTTTTGATTACGGTCACTTCCCGTGTAACAGGGGTAAAACCGTCCAGGGGCAGGGAAAAGGGCAGgagaga                                                                                                                  >  2:19402/1‑140 (MQ=255)                    tCGCCATATTGCTGCAATTCTAGTGTCAAAAAAATGCGATGTTGTTAGGAATTAGTTTATAAACAGACTTTTTTGATTACGGTCACTTCCCGTGTAACAGGGGTAAAACCGTCCAGGGGCAGGGAAAAGGGCAGgagaga                                                                                                                  >  1:538877/1‑140 (MQ=255)                    tCGCCATATTGCTGCAATTCTAGTGTCAAAAAAATGCGATGTTGTTAGGAATTAGTTTATAAACAGACTTTTTTGATTACGGTCACTTCCCGTGTAACAGGGGTAAAACCGTCCAGGGGCAGGGAAAAGGGCAGgagaga                                                                                                                  >  2:284884/1‑140 (MQ=255)                     cGCCATATTGCTGCAATTCTAGTGTCAAAAAAATGCGATGTTGTTAGGAATTAGTTTATAAACAGACTTTTTTGATTACGGTCACTTCCCGTGTAACAGGGGTAAAACCGTCCAGGGGCAGGGAAAAGGGCAGGagagag                                                                                                                 <  2:139065/140‑1 (MQ=255)                      gCCATATTGCTGCAATTCTAGTGTCAAAAAAATGCGATGTTGTTAGGAATTAGTTTATAAACAGACTTTTTTGATTACGGTCACTTCCCGTGTAACAGGGGTAAAACCGTCCAGGGGCAGGGAAAAGGGCAGGAGAGAGc                                                                                                                >  2:1224550/1‑140 (MQ=255)                           atTGCTGCAATTCTAGTGTCAAAAAAATGCGATGTTGTTAGGAATTAGTTTATAAACAGACTTTTTTGATTACGGTCACTTCCCGTGTAACAGGGGTAAAACCGTCCAGGGGCAGGGAAAAGGGCAGGAGAGAGCCGCGt                                                                                                           <  1:408373/140‑1 (MQ=255)                              gctgcAATTCTAGTGTCAAAAAAATGTGATGTTGTTAGGAATTAGTTTATAAACAGACTTTTTTGATTACGGTCACTTCCCGTGTAACAGGGGTAAAACCGTCCAGGGGCAGGGAAAAGGGCAGGAGAGAGCCGCGTCAg                                                                                                        <  2:756206/140‑1 (MQ=255)                               ctgcAATTCTAGTGTCAAAAAAATGCGATGTTGTTAGGAATTAGTTTATAAACAGACTTTTTTGATTACGGTTACTACCCGTGTAACAGGGGTAAAACCGTCCAGGGGCAGGGAAAAGGGCAGGAGAGAGCCGCGTCAGc                                                                                                       >  2:1229914/1‑140 (MQ=255)                               ctgcAATTCTAGTGTCAAAAAAATGCGATGTTGTTAGGAATTAGTTTATAAACAGACTTTTTTGATTACGGTCACTTCCCGTGTAACAGGGGTAAAACCGTCCAGGGGCAGGGAAAAGGGCAGGAGAGAGCCGCGTCAGc                                                                                                       <  2:8957/140‑1 (MQ=255)                                  cAATTCTAGTGTCAAAAAAATGCGATGTTGTTAGGAATTAGTTTATAAACAGACTTTTTTGATTACGGTCACTTCCCGTGTAACAGGGGTAAAACCGTCCAGGGGCAGGGAAAAGGGCAGGAGAGAGCCGCGTCAGCCgg                                                                                                    >  1:1114733/1‑140 (MQ=255)                                    aTTCTAGTGTCAAAAAAATGCGATGTTGTTAGGAATTAGTTTATAAACAGACTTTTTTGATTACGGTCACTTCCCGTGTAACAGGGGTAAAACCGTCCAGGGGCAGGGAAAAGGGCAGGAGAGAGCCGCGTCAGCCGGGt                                                                                                  <  2:1147843/140‑1 (MQ=255)                                    aTTCTAGTGTCAAAAAAATGCGATGTTGTTAGGAATTAGTTTATAAACAGACTTTTTTGATTACGGTCACTTCCCGTGTAACAGGGGTAAAACCGTCCAGGGGCAGGGAAAAGGGCAGGAGAGAGCCGCGTCAGCCGGGt                                                                                                  >  2:140304/1‑140 (MQ=255)                                                         gATGTTGTTAGGAATTAGTTTATAAACAGACTTTTTTGATTACGGTCACTTCCCGTGTAACAGGGGTAAAACCGTCCAGGGGCAGGGAAAAGGGCAGGAGAGAGCCGCGTCAGCCGGGTAACAGCTAAAACGCGGCGttt                                                                             <  1:522345/140‑1 (MQ=255)                                                           tgttgtTAGGAATTAGTTTATAAACAGACTTTTTTGATTACGGTCACTTCCCGTGTAACAGGGGTAAAACCGTCCAGGGGCAGGGAAAAGGGCAGGAGAGAGCCGCGTCAGCCGGGTAACAGCTAAAACGCGGCGTTTcg                                                                           <  1:1005658/140‑1 (MQ=255)                                                           tgttgtTAGGAATTAGTTTATAAACAGACTTTTTTGATTACGGTCACTTCCCGTGTAACAGGGGTAAAACCGTCCAGGGGCAGGGAAAAGGGCAGGAGAGAGCCGCGTCAGCCGGGTAACAGCTAAAACGCGGCGTTTcg                                                                           <  1:227509/140‑1 (MQ=255)                                                              tgttAGGAATTAGTTTATAAACAGACTTTTTTGATTACGGTCACTTCCCGTGTAACAGGGGTAAAACCGTCCAGGGGCAGGGAAAAGGGCAGGAGAGAGCCGCGTCAGCCGGGTAACAGCTAAAACGCGGCGTTTCGCgg                                                                        <  2:152237/140‑1 (MQ=255)                                                              tgttAGGAATTAGTTTATAAACAGACTTTTTTGATTACGGTCACTTCCCGTGTAACAGGGGTAAAACCGTCCAGGGGCAGGGAAAAGGGCAGGAGAGAGCCGCGTCAGCCGGGTAACAGCTAAAACGCGGCGTTTCGCgg                                                                        <  1:12116/140‑1 (MQ=255)                                                                    gAATTAGTTTATAAACAGACTTTTTTGATTACGGTCACTTCCCGTGTAACAGGGGTAAAACCGTCCAGGGGCAGGGAAAAGGGCAGGAGAGAGCCGCGTCAGCCGGGTAACAGCTAAAACGCGGCGTTTCGCGGTTCAgg                                                                  <  1:352393/140‑1 (MQ=255)                                                                       ttAGTTTATAAACAGACTTTTTTGATTACGGTCACTTCCCGTGTAACAGGGGTAAAACCGTCCAGGGGCAGGGAAAAGGGCAGGAGAGAGCCGCGTCAGCCGGGTAACAGCTAAAACGCGGCGTTTCGCGGTTCAGGaaa                                                               >  1:256943/1‑140 (MQ=255)                                                                       ttAGTTTATAAACAGACTTTTTTGATTACGGTCACTTCCCGTGTAACAGGGGTAAAACCGTCCAGGGGCAGGGAAAAGGGCAGGAGAGAGCCGCGTCAGCCGGGTAACAGCTAAAACGCGGCGTTTCGCGGTTCAGGaaa                                                               >  1:749529/1‑140 (MQ=255)                                                                         aGTTTATAAACAGACTTTTTTGATTACGGTCACTTCCCGTGTAACAGGGGTAAAACCGTCCAGGGGCAGGGAAAAGGGCAGGAGAGAGCCGCGTCAGCCGGGTAACAGCTAAAACGCGGCGTTTCGCGGt                                                                       <  2:880373/130‑1 (MQ=255)                                                                         aGTTTATAAACAGACTTTTTTGATTACGGTCACTTCCCGTGTAACAGGGGTAAAACCGTCCAGGGGCAGGGAAAAGGGCAGGAGAGAGCCGCGTCAGCCGGGTAACAGCTAAAACGCGGCGTTTCGCGGt                                                                       >  1:880373/1‑130 (MQ=255)                                                                         aGTTTATAAACAGACTTTTTTGATTACGGTCACTTCCCGTGTAACAGGGGTAAAACCGTCCAGGGGCAGGGAAAAGGGCAGGAGAGAGCCGCGTCAGCCGGGTAACAGCTAAAACGCGGCGTTTCGCGGTTCAGGAAATg                                                             >  1:1044741/1‑140 (MQ=255)                                                                          gTTTATAAACAGACTTTTTTGATTACGGTCACTTCCCGTGTAACAGGGGTAAAACCGTCCAGGGGCAGGGAAAAGGGCAGGAGAGAGCCGCGTCAGCCGGGTAACAGCTAAAACGCGGCGTTTCGCGGTTCAGGAAATGa                                                            <  1:1188057/140‑1 (MQ=255)                                                                             tataAACAGACTTTTTTGATTACGGTCACTTCCCGTGTAACAGGGGTAAAACCGTCCAGGGGCAGGGAAAAGGGCAGGAGAGAGCCGCGTCAGCCGGGTAACAGCTAAAACGCGGCGTTTCGCGGTTCAGGAAATGAAGt                                                         >  1:810832/1‑140 (MQ=255)                                                                              ataAACAGACTTTTTTGATTACGGTCACTTCCCGTGTGACAGGGGTAAAACCGTCCAGGGGCAGGGAAAAGGGCAGGAGAGAGCCGCGTCAGCCGGGTAACAGCTAATACGCGGCGTTTCGCGGTTCAGGAAATGAAGtt                                                        >  2:1247811/1‑140 (MQ=255)                                                                              ataAACAGACTTTTTTGATTACGGTCACTTCCCGTGTAACAGGGGTAAAACCGTCCAGGGGCAGGGAAAAGGGCAGGAGAGAGCCGCGTCAGCCGGGTAACAGCTAAAACGCGGCGTTTCGCGGTTCAGGAAATGAAGtt                                                        >  2:1303086/1‑140 (MQ=255)                                                                               taAACAGACTTTTTTGATTACGGTCACTTCCCGTGTAACAGGGGTAAAACCGTCCAGGGGCAGGGAAAAGGGCAGGAGAGAGCCGCGTCAGCCGGGTAACAGCTAAAACGCGGCGTTTCGCGGTTCAGGAAATGAAGTTc                                                       >  1:1342402/1‑140 (MQ=255)                                                                                  aCAGACTTTTTTGATTACGGTCACTTCCCGTGTAACAGGGGTAAAACCGTCCAGGGGCAGGGAAAAGGGCAGGAGAGAGCCGCGTCAGCCGGGTAACAGCTAAAACGCGGCGTTTCGCGGTTCAGGAAATGAAGTTCAGc                                                    >  1:229117/1‑140 (MQ=255)                                                                                   cAGACTTTTTTGATTACGGTTACTACCCGTGTAACAGGGGTAAAACCGTCCAGGGGCAGGGAAAAGGGCAGGAGAGAGCCGCGTCAGCCGGGTAACAGCTAAAACGCGGCGTTTCGCGGTTCAGGAAATGAAGTTCAGcc                                                   <  1:1229914/140‑1 (MQ=255)                                                                                   cAGACTTTTTTGATTACGGTCACTTCCCGTGTAACAGGGGTAAAACCGTCCAGGGGCAGGGAAAAGGGCAGGAGAGAGCCGCGTCAGCCGGGTAACAGCTAAAACGCGGCGTTTCGCGGTTCAGGAAATGAAGTTCAGcc                                                   <  2:178239/140‑1 (MQ=255)                                                                                   cAGACTTTTTTGATTACGGTCACTTCCCGTGTAACAGGGGTAAAACCGTCCAGGGGCAGGGAAAAGGGCAGGAGAGAGCCGCGTCAGCCGGGTAACAGCTAAAACGCGGCGTTTCGCGGTTCAGGAAATGAAGTTCAGcc                                                   <  1:284884/140‑1 (MQ=255)                                                                                   cAGACTTTTTTGATTACGGTCACTTCCCGTGTAACAGGGGTAAAACCGTCCAGGGGCAGGGAAAAGGGCAGGAGAGAGCCGCGTCAGCCGGGTAACAGCTAAAACGCGGCGTTTCGCGGTTCAGGAAATGAAGTTCAGcc                                                   <  2:809951/140‑1 (MQ=255)                                                                                    ataCTTTTTTGATTACGGTCACTTCCCGTGTAACAGGGGTAAAACCGTCCAGGGGCAGGGAAAAGGGCAGGAGAGAGCCGCGTCAGCCGGGTAACAGCTAAAACGCGGCGTTTCGCGGTTCAGGAAATGAAGTTCAGccc                                                  <  2:916775/138‑1 (MQ=255)                                                                                    aGACTTTTTTGATTACGGTCACTTCCCGTGTAACAGGGGTAAAACCGTCCAGGGGCAGGGAAAAGGGCAGGAGAGAGCCGCGTCAGCCGGGTAACAGCTAAAACGCGGCGTTTCGCGGTTCAGGAAATGAAGTTCAGccc                                                  <  1:206071/140‑1 (MQ=255)                                                                                     gACTTTTTTGATTACGGTCACTTCCCGTGTAACAGGGGTAAAACCGTCCAGGGGCAGGGAAAAGGGCAGGAGAGAGCCGCGTCAGCCGGGTAACAGCTAAAACGCGGCGTTTCGCGGTTCAGGAAATGAAGTTCAGcccc                                                 >  2:1091472/1‑140 (MQ=255)                                                                                     gACTTTTTTGATTACGGTCACTTCCCGTGTAACAGGGGTAAAACCGTCCAGGGGCAGGGAAAAGGGCAGGAGAGAGCCGCGTCAGCCGGGTAACAGCTAAAACGCGGCGTTTCGCGGTTCAGGAAATGAAGTTCAGcccc                                                 >  2:146765/1‑140 (MQ=255)                                                                                          ttttGATTACGGTCACTTCCCGTGTAACAGGGGTAAAACCGTCCAGGGGCAGGGAAAAGGGCAGGAGAGAGCCGCGTCAGCCGGGTAACAGCTAAAACGCGGCGTTTCGCGGTTCAGGAAATGAAGTTCAGCCCCTGttt                                            <  2:229117/140‑1 (MQ=255)                                                                                             tGATTACGGTCACTTCCCGTGTAACAGGGGTAAAACCGTCCAGGGGCAGGGAAAAGGGCAGGAGAGAGCCGCGTCAGCCGGGTAACAGCTAAAACGCGGCGTTTCGCGGTTCAGGAAATGAAGTTCAGCCCCTGTTTTaa                                         <  2:749529/140‑1 (MQ=255)                                                                                               aTTACGGTCACTTCCCGTGTAACAGGGGTAAAACCGTCCAGGGGCAGGGAAAAGGGCAGGAGAGAGCCGCGTCAGCCGGGTAACAGCTAAAACGCGGCGTTTCGCGGTTCAGGAAATGAAGTTCAGCCCCTGTTTTAaca                                       <  1:1224550/140‑1 (MQ=255)                                                                                                 tACGGTCACTTCCCGTGTAACAGGGGTAAAACCGTCCAGGGGCAGGGAAAAGGGCAGGATAGAGCCGCGTCAGCCGGGTAACAGCTAAAACGCGGCGTTTCGCGGTTCAGGAAATGAAGTTCAGCCCCTGTTTTAACAcc                                     <  2:546381/140‑1 (MQ=255)                                                                                                    ggTCACTTCCCGTGTAACAGGGGTAAAACCGTCCAGGGGCAGGGAAAAGGGCAGGAGAGAGCCGCGTCAGCCGGGTAACAGCTAAAACGCGGCGTTTCGCGGTTCAGGAAATGAAGTTCAGCCCCTGTTTTAACACCAGa                                  >  1:691930/1‑140 (MQ=255)                                                                                                       cACTTCCCGTGTAACAGGGGTAAAACCGTCCAGGGGCAGGGAAAAGGGCAGGAGAGAGCCGCGTCAGCCGGGTAACAGCTAAAACGCGGCGTTTCGCGGTTCAGGAAATGAAGTTCAGCCCCTGTTTTAACACCAGATCg                               <  2:343399/140‑1 (MQ=255)                                                                                                          ttCCCGTGTAACAGGGGTAAAACCGTCCAGGGGCAGGGAAAAGGGCAGGAGAGAGCCGCGTCAGCCGGGTAACAGCTAAAACGCGGCGTTTCGCGGTTCAGGAAATGAAGTTCAGCCCCTGTTTTAACACCAGATCGCAg                            >  1:262216/1‑140 (MQ=255)                                                                                                              cGTGTAACAGGGGTAAAACCGTCCAGGGGCAGGGAAAAGGGCAGGAGAGAGCCGCGTCAGCCGGGTAACAGCTAAAACGCGGCGTTTCGCGGTTCAGGAAATGAAGTTCAGCCCCTGTTTTAACACCAGATCGCAGGCtt                        <  2:857186/140‑1 (MQ=255)                                                                                                                tgtAACAGGGGTAAAACCGTCCAGGGGCAGGGAAAAGGGCAGGAGAGAGCCGCGTCAGCCGGGTAACAGCTAAAACGCGGCGTTTCGCGGTTCAGGAAATGAAGTTCAGCCCCTGTTTTAacac                                      <  1:506787/124‑1 (MQ=255)                                                                                                                tgtAACAGGGGTAAAACCGTCCAGGGGCAGGGAAAAGGGCAGGAGAGAGCCGCGTCAGCCGGGTAACAGCTAAAACGCGGCGTTTCGCGGTTCAGGAAATGAAGTTCAGCCCCTGTTTTAacac                                      >  2:506787/1‑124 (MQ=255)                                                                                                                tgtAACAGGGGTAAAACCGTCCAGGGGCAGGGAAAAGGGCAGGAGAGAGCCGCGTCAGCCGGGTAACAGCTAAAACGCGGCGTTTCGCGGTTCAGGAAATGAAGTTCAGCCCCTGTTTTAACACCAGATCGCAGGCTTTg                      >  1:144215/1‑140 (MQ=255)                                                                                                                  tAACAGGGGTAAAACCGTCCAGGGGCAGGGAAAAGGGCAGGAGAGAGCCGCGTCAGCCGGGTAACAGCTAAAACGCGGCGTTTCGCGGTTCAGGAAATGAAGTTCAGCCCCTGTTTTAACACCAGATCGCAGGCTTTGGt                    >  2:726370/1‑140 (MQ=255)                                                                                                                   aaCAGGGGTAAAACCGTCCAGGGGCAGGGAAAAGGGCAGGAGAGAGCCGCGTCAGCCGGGTAACAGCTAAAACGCGGCGTTTCGCGGTTCAGGAAATGAAGTTCAGCCCCTGTTTTAACACCAGATCGCAGGCTTTGGtt                   <  2:1044741/140‑1 (MQ=255)                                                                                                                    aCAGGGGTAAAACCGTCCAGGGGCAGGGAAAAGGGCAGGAGAGAGCCGCGTCAGCCGGGTAACAGCTAAAACGCGGCGTTTCGCGGTTCAGGAAATGAAGTTCAGCCCCTGTTTTAACACCAGATCGCAGGCTTTGGttt                  >  1:377503/1‑140 (MQ=255)                                                                                                                       ggggTAAAACCGTCCAGGGGCAGGGAAAAGGGCAGGAGAGAGCCGCGTCAGCCGGGTAACAGCTAAAACGCGGCGTTTCGCGGTTCAGGAAATGAAGTTCAGCCCCTGTTTTAACACCAGATCGCAGGCTTTGGTTTTCa               >  2:325038/1‑140 (MQ=255)                                                                                                                        gggTAAAACCGTCCAGGGGCAGGGAAAAGGGCAGGAGAGAGCCGCGTCAGCCGGGTAACAGCTAAAACGCGGCGTTTCGCGGTTCAGGAAATGAAGTTCAGCCCCTGTTTTAACACCAGATCGCAGGCTTTGGtt                   >  2:686930/1‑135 (MQ=255)                                                                                                                        gggTAAAACCGTCCAGGGGCAGGGAAAAGGGCAGGAGAGAGCCGCGTCAGCCGGGTAACAGCTAAAACGCGGCGTTTCGCGGTTCAGGAAATGAAGTTCAGCCCCTGTTTTAACACCAGATCGCAGGCTTTGGtt                   <  1:686930/135‑1 (MQ=255)                                                                                                                             aaaCCGTCCAGGGGCAGGGAAAAGGGCAGGAGAGAGCCGCGTCAGCCGGGTAACAGCTAAAACGCGGCGTTTCGCGGTTCAGGAAATGAAGTTCAGCCCCTGTTTTAACACCAGATCGCAGGCTTTGGTTTTCACCtttt         >  2:1214627/1‑140 (MQ=255)                                                                                                                                 cGTCCAGGGGCAGGGAAAAGGGCAGGAGAGAGCCGCGTCAGCCGGGTAACAGCTAAAACGCGGCGTTTCGCGGTTCAGGAAATGAAGTTCAGCCCCTGTTTTAACACCAGATCGCAGGCTTTGGTTTTCACCTTTTCTGc     <  2:1145766/140‑1 (MQ=255)                                                                                                                                  gTCCAGGGGCAGGGAAAAGGGCAGGAGAGAGCCGCGTCAGCCGGGTAACAGCTAAAACGCGGCGTTTCGCGGTTCAGGAAATGAAGTTCAGCCCCTGTTTTAACACCAGATCGCAGGCTTTGGTTTTCACCTTTTCTGcc    >  1:210846/1‑140 (MQ=255)                                                                                                                                   tCCAGGGGCAGGGAAAAGGGCAGGAGAGAGCCGCGTCAGCCGGGTAACAGCTAAAACGCGGCGTTTCGCGGTTCAGGAAATGAAGTTCAGCCCCTGTTTTAACACCAGATCGCAGGCTTTGGTTTTCACCTTTTCTGc     <  1:340174/138‑1 (MQ=255)                                                                                                                                   tCCAGGGGCAGGGAAAAGGGCAGGAGAGAGCCGCGTCAGCCGGGTAACAGCTAAAACGCGGCGTTTCGCGGTTCAGGAAATGAAGTTCAGCCCCTGTTTTAACACCAGATCGCAGGCTTTGGTTTTCACCTTTTCTGc     >  2:340174/1‑138 (MQ=255)                                                                                                                                   tCCAGGGGCAGGGAAAAGGGCAGGAGAGAGCCGCGTCAGCCGGGTAACAGCTAAAACGCGGCGTTTCGCGGTTCAGGAAATGAAGTTCAGCCCCTGTTTTAACACCAGATCGCAGGCTTTGGTTTTCACCTTTTCTGCCa   >  2:1232275/1‑140 (MQ=255)                                                                                                                                   tCCAGGGGCAGGGAAAAGGGCAGGAGAGAGCCGCGTCAGCCGGGTAACAGCTAAAACGCGGCGTTTCGCGGTTCAGGAAATGAAGTTCAGCCCCTGTTTTAACACCAGATCGCAGGCTTTGGTTTTCACCTTTTCTGCCa   >  2:907125/1‑140 (MQ=255)                                                                                                                                   tCCAGGGGCAGGGAAAAGGGCAGGAGAGAGCCGCGTCAGCCGGGTAACAGCTAAAACGCGGCGTTTCGCGGTTCAGGAAATGAAGTTCAGCCCCTGTTTTAACACCAGATCGCAGGCTTTGG‑TTTCACCGTTTCTGCCAg  >  1:1315091/1‑140 (MQ=255)                                                                                                                                    ccAGGGGCAGGGAAAAGGGCAGGAGAGAGCCGCGTCAGCCGGGTAACAGCTAAAACGCGGCGTTTCGCGGTTCAGGAAATGAAGTTCAGCCCCTGTTTTAACACCAGATCGCAGGCTTTGGTTTTCACCTTTTCTGCCAg  >  1:605892/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                       GAAGCACAATGTAATGGCGTCGCCATATTGCTGCAATTCTAGTGTCAAAAAAATGCGATGTTGTTAGGAATTAGTTTATAAACAGACTTTTTTGATTACGGTCACTTCCCGTGTAACAGGGGTAAAACCGTCCAGGAGCAGGGAAAAGGGCAGGAGAGAGCCGCGTCAGCCGGGTAACAGCTAAAACGCGGCGTTTCGCGGTTCAGGAAATGAAGTTCAGCCCCTGTTTTAACACCAGATCGCAGGCTTTGGTTTTCACCTTTTCTGCCAG  >  NC_000913/4599493‑4599763

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑