Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,016,849	C→T	G484D (GGC→GAC)	ycbZ ←	putative ATP‑dependent protease YcbZ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,016,849	0	C	T	100.0%	263.4 / NA	76	G484D (GGC→GAC)	ycbZ	putative ATP‑dependent protease YcbZ
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (46/30);  total (46/30)

AGATGGTGAATTTGCCTTCTTCTACCGCTTTCACCAGTTCACTGTGAAGACTTAAATGGCGAACGTTAGCTGTGGGGATAATGACACCTTGTTTCCCGGTTAACTCACGTTGCTGGCAAATAGCAAAGAAGCCTTCGATTTTCTCATTTAAACCACCGACCGGCTGGGCGCGACCGAACTGATCGACTGAACCTGTGATAGCGATACTCTGATTCACCGGCACATCGGCGAGGGCGCTTATCAGGGCGCAGAGTTCAGCCATCGA  >  NC_000913/1016718‑1016982                                                                                                                                    |                                                                                                                                      aGATGGTGAATTTGCCTTCTTCTACCGCTTTCACCAGTTCACTGTGAAGACTTAAATGGCGAACGTTAGCTGTGGGGATAATTACACCTTGTTTCCCGGTTAACTCACGTTGCTGGCAAATAGCAAAGAAGTCTTCGAtt                                                                                                                               >  1:648584/1‑140 (MQ=255)   aTGGTGAATTTGCCTTCTTCTACCGCTTTCACCAGTTCACTGTGAAGACTTAAATGGCGAACGTTAGCTGTGGGGATAATGACACCTTGTTTCCCGGTTAACTCACGTTGCTGGCAAATAGCAAAGAAGTCTTCGAtttt                                                                                                                             <  1:647675/140‑1 (MQ=255)        gAATTTGCCTTCTTCTACCGCTTTCACCAGTTCACTGTGAAGACTTAAATGGCGAACGTTAGCTGTGGGGATAATGACACCTTGTTTCCCGGTTAACTCACGTTGCTGGCAAATAGCAAAGAAGTCTTCGATTTTCTCAt                                                                                                                        >  1:590884/1‑140 (MQ=255)         aaTTTGCCTTCTTCTACCGCTTTCACCAGTTCACTGTGAAGACTTAAATGGCGAACGTTAGCTGTGGGGATAATGACACCTTGTTTCCCGGTTAACTCACGTTGCTGGCAAATAGCAAAGAAGTCTTCGATTTTCTCAtt                                                                                                                       >  2:179349/1‑140 (MQ=255)               ccTTCTTCTACCGCTTTCACCAGTTCACTGTGAAGACTTAAATGGCGAACGTTAGCTGTGGGGATAATGACACCTTGTTTCCCGGTTAACTCACGTTGCTGGCAAATAGCAAAGAAGTCTTCGATTTTCTCATTTAAacc                                                                                                                 >  1:1193631/1‑140 (MQ=255)                cttcttCTACCGCTTTCACCAGTTCACTGTGAAGACTTAAATGGCGAACGTTAGCTGTGGGGATAATGACACCTTGTTTCCCGGTTAACTCACGTTGCTGGCAAATAGCAAAGAAGTCTTCGATTTTCTCATTTAAacca                                                                                                                >  1:989869/1‑140 (MQ=255)                   cttctACCGCTTTCACCAGTTCACTGTGAAGACTTAAATGGCGAACGTTAGCTGTGGGGATAATGACACCTTGTTTCCCGGTTAACTCACGTTGCTGGCAAATAGCAAAGAAGTCTTCGATTTTCTCATTTAAACCaccg                                                                                                             >  1:531140/1‑140 (MQ=255)                   cttctACCGCTTTCACCAGTTCACTGTGAAGACTTAAATGGCGAACGTTAGCTGTGGGGATAATGACACCTTGTTTCCCGGTTAACTCACGTTGCTGGCAAATAGCAAAGAAGTCTTCGATTTTCTCATTTAAACCaccg                                                                                                             >  2:1329685/1‑140 (MQ=255)                      ctACCGCTTTCACCAGTTCACTGTGAAGACTTAAATGGCGAACGTTAGCTGTGGGGATAATGACACCTTGTTTCCCGGTTAACTCACGTTGCTGGCAAATAGCAAAGAAGTCTTCGATTTTCTCATTTAAACCaccgacc                                                                                                          >  1:805253/1‑140 (MQ=255)                         ccGCTTTCACCAGTTCACTGTGAAGACTTAAATGGCGAACGTTAGCTGTGGGGATAATGACACCTTGTTTCCCGGTTAACTCACGTTGCTGGCAAATAGCAAAGAAGTCTTCGATTTTCTCATTTAAACCACCGACCGGc                                                                                                       >  2:1269051/1‑140 (MQ=255)                            cTTTCACCAGTTCACTGTGAAGACTTAAATGGCGAACGTTAGCTGTGGGGATAATGACACCTTGTTTCCCGGTTAACTCACGCTGCTGGCAAATAGCAAAGAAGTCTTCGATTTTCTCATTTAAACCACCGACCGGCTgg                                                                                                    >  2:241500/1‑140 (MQ=255)                             tttCACCAGTTCTCTGTGAAGACTTAAATGGCGAACGTTAGCTGTGGGGATAATGACACCTTGTTTCCCGGTTAACTCACGTTGCTGGCAAATAGCAAAGAAGTCTTCGATTTTCTCATTTAAACCACCGACCGGCTggg                                                                                                   <  2:1140215/140‑1 (MQ=255)                              ttCACCAGTTCACTGTGAAGACTTAAATGGCGAACGTTAGCTGTGGGGATAATGACACCTTGTTTCCCGGTTAACTCACGTTGCTGGCAAATAGCAAAGAAGTCTTCGATTTTCTCATTTAAACCACCGACCGGCTGGgc                                                                                                  >  2:151487/1‑140 (MQ=255)                               tCACCAGTTCACTGTGAAGACTTAAATGGCGAACGTTAGCTGTGGGGATAATGACACCTTGTTTCCCGGTTAACTCACGTTGCTGGCAAATAGCAAAGAAGTCTTCGATTTTCTCATTTAAACCACCGACCGGCTGGgcg                                                                                                 >  2:211987/1‑140 (MQ=255)                                 aCCAGTTCACTGTGAAGACTTAAATGGCGAACGTTAGCTGTGGGGATAATGACACCTTGTTTCCCGGTTAACTCACGTTGCTGGCAAATAGCAAAGAAGTCTTCGATTTTCTCATTTAAACCACCGACCGGCTGGGcgcg                                                                                               >  1:11708/1‑140 (MQ=255)                                  ccAGTTCACTGTGAAGACTTAAATGGCGAACGTTAGCTGTGGGGATAATGACACCTTGTTTCCCGGTTAACTCACGTTGCTGGCAAATAGCAAAGAAGTCTTCGATTTTCTCATTTAAACCACCGACCGGCTGGGCGCGa                                                                                              <  1:1322676/140‑1 (MQ=255)                                  ccAGTTCACTGTGAAGACTTAAATGGCGAACGTTAGCTGTGGGGATAATGACACCTTGTTTCCCGGTTAACTCACGTTGCTGGCAAATAGCAAAGAAGTCTTCGATTTTCTCATTTAAACCACCGACCGGCTGGGCGCGa                                                                                              <  2:1396097/140‑1 (MQ=255)                                     gTTCACTGTGAAGACTTAAATGGCGAACGTTAGCTGTGGGGATAATGACACCTTGTTTCCCGGTTAACTCACGTTGCTGGCAAATAGCAAAGAAGTCTTCGATTTTCTCATTTAAACCACCGACCGGCTGGGCGCGACCg                                                                                           <  1:211987/140‑1 (MQ=255)                                     gTTCACTGTGAAGACTTAAATGGCGAACGTTAGCTGTGGGGATAATGACACCTTGTTTCCCGGTTAACTCACGTTGCTGGCAAATAGCAAAGAAGTCTTCGATTTTCTCATTTAAACCACCGACCGGCTGGGCGCGACCg                                                                                           <  1:216857/140‑1 (MQ=255)                                      ttCACTGTGAAGACTTAAATGGCGAACGTTAGCTGTGGGGATAATGACACCTTGTTTCCCGGTTAACTCACGTTGCTGGCAAATAGCAAAGAAGTCTTCGATTTTCTCATTTAAACCACCGACCGGCTGGGCGCGACCGa                                                                                          >  1:272877/1‑140 (MQ=255)                                         aCTGTGAAGACTTAAATGGCGAACGTTAGCTGTGGGGATAATGACACCTTGTTTCCCGGTTAACTCACGTTGCTGGCAAATAGCAAAGAAGTCTTCGATTTTCTCATTTAAACCACCGACCGGCTGGGCGCGACCGAACt                                                                                       >  2:681155/1‑140 (MQ=255)                                           tgtgAAGACTTAAATGGCGAACGTTAGCTGTGGGGATAATGACACCTTGTTTCCCGGTTAACTCACGTTGCTGGCAAATAGCAAAGAAGTCTTCGATTTTCTCATTTAAACCACCGACCGGCTGGGCGCGACCGAACTGa                                                                                     >  1:216399/1‑140 (MQ=255)                                            gtgAAGACTTAAATGGCGAACGTTAGCTGTGGGGATAATGACACCTTGTTTCCCGGTTAACTCACGTTGCTGGCAAATAGCAAAGAAGTCTTCGATTTCCTCATTTAAACCACCGACCGGCTGGGCGCGACCGAACTGAt                                                                                    <  1:912635/140‑1 (MQ=255)                                               aaGACTTAAATGGCGAACGTTAGCTGTGGGGATAATGACACCTTGTTTCCCGGTTAACTCACGTTGCTGGCAAATAGCAAAGAAGTCTTCGATTTTCTCATTTAAACCACCGACCGGCTGGGCGCGACCGAACTGa                                                                                     >  2:670899/1‑136 (MQ=255)                                               aaGACTTAAATGGCGAACGTTAGCTGTGGGGATAATGACACCTTGTTTCCCGGTTAACTCACGTTGCTGGCAAATAGCAAAGAAGTCTTCGATTTTCTCATTTAAACCACCGACCGGCTGGGCGCGACCGAACTGa                                                                                     <  1:670899/136‑1 (MQ=255)                                                aGACTTAAATGGCGAACGTTAGCTGTGGGGATAATGACACCTTGTTTCCCGGTTAACTCACGTTGCTGGCAAATAGCAAAGAAGTCTTCGATTTTCTCATTTAAACCACCGACCGGCTGGGCGCGACCGAACTGATCGAc                                                                                >  1:785976/1‑140 (MQ=255)                                                aGACTTAAATGGCGAACGTTAGCTGTGGGGATAATGACACCTTGTTTCCCGGTTAACTCACGTTGCTGGCAAATAGCAAAGAAGTCTTCGATTTTCTCATTTAAACCACCGACCGGCTGGGCGCGACCGAACTGATCGAc                                                                                >  1:588408/1‑140 (MQ=255)                                                aGACTTAAATGGCGAACGTTAGCTGTGGGGATAATGAAACCTTGTTTCCCGGTTAACTCACGTTGCTGGCAAATAGCAAAGAAGTCTTCGATTTTCTCATTTAAACCACCGACCGGCTGGGCGCGACCGAACTGATCGAc                                                                                >  2:1246290/1‑140 (MQ=255)                                                 gACTTAAATGGCGAACGTTAGCTGTGGGGATAATGACACCTTGTTTCCCGGTTAACTCACGTTGCTGGCAAATAGCAAAGAAGTCTTCGATTTTCTCATTTAAACCACCGACCGGCTGGGCGCGACCGAACTGATCGACt                                                                               <  2:1208773/140‑1 (MQ=255)                                                 gACTTAAATGGCGAACGTTAGCTGTGGGGATAATGACACCTTGTTTCCCGGTTAACTCACGTTGCTGGCAAATAGCAAAGAAGTCTTCGATTTTCTCATTTAAACCACCGACCGGCTGGGCGCGACCGAACTGATCGACt                                                                               <  1:823983/140‑1 (MQ=255)                                                   cTTAAATGGCGAACGTTAGCTGTGGGGATAATGACACCTTGTTTCCCGGTTAACTCACGTTGCTGGCAAATAGCAAAGAAGTCTTCGATTTTCTCATTTAAACCACCGACCGGCTGGGCGCGACCGAACTGATCGACTGa                                                                             >  1:857774/1‑140 (MQ=255)                                                    ttAAATGGCGAACGTTAGCTGTGGGGATAATGACACCTTGTTTCCCGGTTAACTCACGTTGCTGGCAAATAGCAAAGAAGTCTTCGATTTTCTCATTTAAACCACCGACCGGCTGGGCGCGACCGAACTGATCGACTGaa                                                                            <  1:179349/140‑1 (MQ=255)                                                     tAAATGGCGAACGTTAGCTGTGGGGATAATGACACCTTGTTTCCCGGTTAACTCACGTTGCTGGCAAATAGCAAAGAAGTCTTCGATTTTCTCATTTAAACCACCGACCGGCTGGGCGCGACCGAACTGATCGACTGAAc                                                                           <  2:590884/140‑1 (MQ=255)                                                     tAAATGGCGAACGTTAGCTGTGGGGATAATGACACCTTGTTTCCCGGTTAACTCACGTTGCTGGCAAATAGCAAAGAAGTCTTCGATTTTCTCATTTAAACCACCGACCGGCTGGGCGCGACCGAACTGATCGACTGAAc                                                                           <  2:989869/140‑1 (MQ=255)                                                       aaTGGCGAACGTTAGCTGTGGGGATAATGACACCTTGTTTCCCGGTTAACTCACGTTGCTGGCAAATAGCAAAGAAGTCTTCGATTTTCTCATTTAAACCACCGACCGGCTGGGCGCGACCGAACTGATCGa                                                                                 >  2:1389256/1‑132 (MQ=255)                                                       aaTGGCGAACGTTAGCTGTGGGGATAATGACACCTTGTTTCCCGGTTAACTCACGTTGCTGGCAAATAGCAAAGAAGTCTTCGATTTTCTCATTTAAACCACCGACCGGCTGGGCGCGACCGAACTGATCGa                                                                                 <  1:1389256/132‑1 (MQ=255)                                                       aaTGGCGAACGTTAGCTGTGGGGATAATGACACCTTGTTTCCCGGTTAACTCACGTTGCTGGCAAATAGCAAAGAAGTCTTCGATTTTCTCATTTAAACCACCGACCGGCTGGGCGCGACCGAACTGATCGACTGAACCt                                                                         >  2:972896/1‑140 (MQ=255)                                                       aaTGGCGAACGTTAGCTGTGGGGATAATGACACCTTGTTTCCCGGTTAACTCACGTTGCTGGCAAATAGCAAAGAAGTCTTCGATTTTCTCATTTAAACCACCGACCGGCTGGGCGCGACCGAACTGATCGACTGAACCt                                                                         >  1:554917/1‑140 (MQ=255)                                                         tGGCGAACGTTAGCTGTGGGGATAATGACACCTTGTTTCCCGGTTAACTCACGTTGCT‑GCAAATAGCAAAGAAGTCTTCGATTTTCTCATTTAAACCACCGACCGGCTGGGCGCGACCGAACTGATCGACTGAACCtgtg                                                                      >  2:309782/1‑140 (MQ=255)                                                           gCGAACGTTAGCTGTGGGGATAATGACACCTTGTTTCCCGGTTAACTCACGTTGCTGGCAAATAGCAAAGAAGTCTTCGATTTTCTCATTTAAACCACCGACCGGCTGGGCGCGACCGAACTGATCGACTGAACCTGTGa                                                                     <  1:1329685/140‑1 (MQ=255)                                                                 gTTAGCTGTGGGGATAATGACACCTTGTTTCCCGGTTAACTCACGTTGCTGGCAAATAGCAAAGAAGTCTTCGATTTTCTCATTTAAACCACCGACCGGCTGGGCGCGACCGAACTGATCGACTGAACCTGTGATAGCGa                                                               <  2:531140/140‑1 (MQ=255)                                                                 gTTAGCTGTGGGGATAATGACACCTTGTTTCCCGGTTAACTCACGTTGCTGGCAAATAGCAAAGAAGTCTTCGATTTTCTCATTTAAACCACCGACCGGCTGGGCGCGACCGAACTGATCGACTGAACCTGTGATAGCGa                                                               <  2:785976/140‑1 (MQ=255)                                                                 gTTAGCTGTGGGGATAATGACACCTTGTTTCCCGGTTAACTCACGTTGCTGGCAAATAGCAAAGAAGTCTTCGATTTTCTCATTTAAACCACCGACCGGCTGGGCGCGACCGAACTGATCGACTGAACCTGTGATAGCGa                                                               <  2:272877/140‑1 (MQ=255)                                                                 gTTAGCTGTGGGGATAATGACACCTTGTTTCCCGGTTAACTCACGTTGCTGGCAAATAGCAAAGAAGTCTTCGATTTTCTCATTTAAACCACCGACCGGCTGGGCGCGACCGAACTGATCGACTGAACCTGTGATAGCGa                                                               <  2:210498/140‑1 (MQ=255)                                                                 gTTAGCTGTGGGGATAATGACACCTTGTTTCCCGGTTAACTCACGTTGCTGGCAAATAGCAAAGAAGTCTTCGATTTTCTCATTTAAACCACCGACCGGCTGGGCGCGACCGAACTGATCGACTGAACCTGTGATAGCGa                                                               <  1:681155/140‑1 (MQ=255)                                                                 gTTAGCTGTGGGGATAATGACACCTTGTTTCCCGGTTAACTCACGTTGCTGGCAAATAGCAAAGAAGTCTTCGATTTTCTCATTTAAACCACCGACCGGCTGGGCGCGACCGAACTGATCGACTGAACCTGTGATAGCGa                                                               <  2:805253/140‑1 (MQ=255)                                                                   tAGCTGTGGGGATAATGACACCTTGTTTCCCGGTTAACTCACGTTGCTGGCAAATAGCAAAGAAGTCTTCGATTTTCTCATTTAAACCACCGACCGGCTGGGCGCGACCGAACTGATCGACTGAACCTGTGATAGCGATa                                                             <  2:476984/140‑1 (MQ=255)                                                                    aGCTGTGGGGATAATGACACCTTGTTTCCCGGTTAACTCACGTTGCTGGCAAATAGCAAAGAAGTCTTCGATTTTCTCATTTAAACCACCGACCGGCTGGGCGCGACCGAACTGATCGACTGAACCTGTGATAGCGATAc                                                            <  1:1035599/140‑1 (MQ=255)                                                                           gggATAATGACACCTTGTTTCCCGGTTAACTCACGTTGCTGGCAAATAGCAAAGAAGTCTTCGATTTTCTCATTTAAACCACCGACCGGCTGGGCGCGACCGAACTGATCGACTGAACCTGTGATAGCGATACTCTGAtt                                                     <  2:881637/140‑1 (MQ=255)                                                                                aaTGACACCTTGTTTCCCGGTTAACTCACGTTGCTGGCAAATAGCAAAGAAGTCTTCGATTTTCTCATTTAAACCACCGACCGGCTGGGCGCGACCGAACTGATCGACTGAACCTGTGATAGCGATACTCTGATTCACCg                                                >  1:1221683/1‑140 (MQ=255)                                                                                   gACACCTTGTTTCCCGGTTAACTCACGTTGCTGGCAAATAGCAAAGAAGTCTTCGATTTTCTCATTTAAACCACCGACCGGCTGGGCGCGACCGAACTGATCGACTGAACCTGTGATAGCGATACTCTGATTCACCGGca                                             >  1:1289670/1‑140 (MQ=255)                                                                                   gACACCTTGTTTCCCGGTTAACTCACGTTGCTGGCAAATAGCAAAGAAGTCTTCGATTTTCTCATTTAAACCACCGACCGGCTGGGCGCGACCGAACTGATCGACTGAACCTGTGATAGCGATACTCTGATTCACCGGca                                             >  2:635574/1‑140 (MQ=255)                                                                                     cacCTTGTTTCCCGGTTAACTCACGTTGCTGGCAAATAGCAAAGAAGTCTTCGATTTTCTCATTTAAACCACCGACCGGCTGGGCGCGACCGAACTGATCGACTGAACCTGTGATAGCGATACTCTGATTCACCGGcaca                                           >  1:568931/1‑140 (MQ=255)                                                                                      acCTTGTTTCCCGGTTAACTCACGTTGCTGGCAAATAGCAAAGAAGTCTTCGATTTTCTCATTTAAACCACCGACCGGCTGGGCGCGACCGAACTGATCGACTGAACCTGTGATAGCGATACTCTGATTCACCGGCACAt                                          >  1:171501/1‑140 (MQ=255)                                                                                        cTTGTTTCCCGGTTAACTCACGTTGCTGGCAAATAGCAAAGAAGTCTTCGATTTTCTCATTTAAACCACCGACCGGCTGGGCGCGACCGAACTGATCGACTGAACCTGTGATAGCGATACTCTGATTCACCGGCACATCg                                        >  2:826772/1‑140 (MQ=255)                                                                                        cTTGTTTCCCGGTTAACTCACGTTGCTGGCAAATAGCAAAGAAGTCTTCGATTTTCTCATTTAAACCACCGACCGGCTGGGCGCGACCGAACTGATCGACTGAACCTGTGATAGCGATACTCTGATTCACCGGCACATCg                                        >  2:626272/1‑140 (MQ=255)                                                                                          tGTTTCCCGGTTAACTCACGTTGCTGGCAAATAGCAAAGAAGTCTTCGATTTTCTCATTTAAACCACCGACCGGCTGGGCGCGACCGAACTGATCGACTGAACCTGTGATAGCGATACTCTGATTCACCGGCACATCGGc                                      >  2:1084228/1‑140 (MQ=255)                                                                                            tttCCCGGTTAACTCACGTTGCTGGCAAATAGCAAAGAAGTCTTCGATTTTCTCATTTAAACCACCGACCGGCTGGGCGCGACCGAACTGATCGACTGAACCTGTGATAGCGATACTCTGATTCACCGGCACATCGGCGa                                    >  2:1182832/1‑140 (MQ=255)                                                                                            tttCCCGGTTAACTCACGTTGCTGGCAAATAGCAAAGAAGTCTTCGATTTTCTCATTTAAACCACCGACCGGCTGGGCGCGAACGAACTGATCGACTGAACCTGTGATAGCGATACTCTGATTCACCGGCACATCGGCGa                                    <  1:1397446/140‑1 (MQ=255)                                                                                               cccGGTTAACTCACGTTGCTGGCAAATAGCAAAGAAGTCTTCGATTTTCTCATTTAAACCACCGACCGGCTGGGCGCGACCGAACTGATCGACTGAACCTGTGATAGCGATACTCTGATTCACCGGCACATCGGCGAggg                                 >  2:1098162/1‑140 (MQ=255)                                                                                               cccGGTTAACTCACGTTGCTGGCAAATAGCAAAGAAGTCTTCGATTTTCTCATTTAAACCACCGACCGGCTGGGCGCGACCGAACTGATCGACTGAACCTGTGATAGCGATACTCTGATTCACCGGCACATCGGCGAggg                                 <  2:648584/140‑1 (MQ=255)                                                                                                ccGGTTAACTCACGTTGCTGGCAAATAGCAAAGAAGTCTTCGATTTTCTCATTTAAACCACCGACCGGCTGGGCGCGACCGAACTGATCGACTGAACCTGTGATAGCGATACTCTGATTCACCGGCACATCGGCGAGGgc                                >  2:155366/1‑140 (MQ=255)                                                                                                ccGGTTAACTCACGTTGCTGGCAAATAGCAAAGAAGTCTTCGATTTTCTCATTTAAACCACCGACCGGCTGGGCGCGACCGAACTGATCGACTGAACCTGTGATAGCGATACTCTGATTCACCGGCACATCGGCGAGGgc                                >  2:1288377/1‑140 (MQ=255)                                                                                                  ggTTAACTCACGTTGCTGGCAAATAGCAAAGAAGTCTTCGATTTTCTCATTTAAACCACCGACCGGCTGGGCGCGACCGAACTGATCGACTGAACCTGTGATAGCGATACTCTGATTCACCGGCACATCGGCGAGGgcgc                              >  2:840922/1‑140 (MQ=255)                                                                                                   gTTAACTCACGTTGCTGGCAAATAGCAAAGAAGTCTTCGATTTTCTCATTTAAACCACCGACCGGCTGGGCGCGACCGAACTGATCGACTGAACCTGTGATAGCGATACTCTGATTCACCGGCACATCGGCGAGGGCGCt                             >  2:923463/1‑140 (MQ=255)                                                                                                    ttAACTCACGTTGCTGGCAAATAGCAAAGAAGTCTTCGATTTTCTCATTTAAACCACCGACCGGCTGGGCGCGACCGAACTGATCGACTGAACCTGTGATAGCGATACTCTGATTCACCGGCACATCGGCGAGGGCGCtt                            >  1:1269413/1‑140 (MQ=255)                                                                                                      aaCTCACGTTGCTGGCAAATAGCAAAGAAGTCTTCGATTTTCTCATTTAAACCACCGACCGGCTGGGCGCGACCGAACTGATCGACTGAACCTGTGATAGCGATACTCTGATTCACCGGCACATCGGCGAGGGCGCTTAt                          >  1:701078/1‑140 (MQ=255)                                                                                                      aaCTCACGTTGCTGGCAAATAGCAAAGAAGTCTTCGATTTTCTCATTTAAACCACCGACCGGCTGGGCGCGACCGAACTGATCGACTGAACCTGTGATAGCGATACTCTGATTCACCGGCACATCGGCGAGGGCGCTTAt                          >  1:1153166/1‑140 (MQ=255)                                                                                                        cTCACGTTGCTGGCAAATAGCAAAGAAGTCTTCGATTTTCTCATTTAAACCACCGACCGGCTGGGCGCGACCGAACTGATCGACTGAACCTGTGATAGCGATACTCTGATTCACCGGCACATCGGCGAGGGCGCTTATCa                        <  1:1182832/140‑1 (MQ=255)                                                                                                              ttGCTGGCAAATAGCAAAGAAGTCTTCGATTTTCTCATTTAAACCACCGACCGGCTGGGCGCGACCGAACTGATCGACTGAACCTGTGATAGCGATACTCTGATTCACCGGCACATCGGCGAGGGCGCTTATCAGGgcgc                  <  2:1308174/140‑1 (MQ=255)                                                                                                                 cTGGCAAATAGCAAAGAAGTCTTCGATTTTCTCATTTAAACCACCGACCGGCTGGGCGCGACCGAACTGATCGACTGAACCTGTGATAGCGATACTCTGATTCACCGGCACATCGGCGAGGGCGCTTATCAGGGCGCaga               >  1:977374/1‑140 (MQ=255)                                                                                                                     cAAATAGCAAAGAAGTCTTCGATTTTCTCATTTAAACCACCGACCGGCTGGGCGCGACCGAACTGATCGACTGAACCTGTGATAGCGATACTCTGATTCACCGGCACATCGGCGAGGGCGCTTATCAGGGCGCAGAGTTc           >  2:28320/1‑140 (MQ=255)                                                                                                                              aagaagTCTTCGATTTTCTCATTTAAACCACCGACCGGCTGGGCGCGACCGAACTGATCGACTGAACCTGTGATAGCGATACTCTGATTCACCGGCACATCGGCGAGGGCGCTTATCAGGGCGCa                 >  2:116761/1‑125 (MQ=255)                                                                                                                              aagaagTCTTCGATTTTCTCATTTAAACCACCGACCGGCTGGGCGCGACCGAACTGATCGACTGAACCTGTGATAGCGATACTCTGATTCACCGGCACATCGGCGAGGGCGCTTATCAGGGCGCa                 <  1:116761/125‑1 (MQ=255)                                                                                                                              aagaagTCTTCGATTTTCTCATTTAAACCACCGACCGGCTGGGCGCGACCGAACTGATCGACTGAACCTGTGATAGCGATACTCTGATTCACCGGCACATCGGCGAGGGCGCTTATCAGGGCGCAGAGTTCAGCCATCGa  <  2:568931/140‑1 (MQ=255)                                                                                                                              aagaagTCTTCGATTTTCTCATTTAAACCACCGACCGGCTGGGCGCGACCGAACTGATCGACTGAACCTGTGATAGCGATACTCTGATTCACCGGCACATCGGCGAGGGCGCTTATCAGGGCGCAGAGTTCAGCCATCGa  >  1:142977/1‑140 (MQ=255)                                                                                                                                    |                                                                                                                                      AGATGGTGAATTTGCCTTCTTCTACCGCTTTCACCAGTTCACTGTGAAGACTTAAATGGCGAACGTTAGCTGTGGGGATAATGACACCTTGTTTCCCGGTTAACTCACGTTGCTGGCAAATAGCAAAGAAGCCTTCGATTTTCTCATTTAAACCACCGACCGGCTGGGCGCGACCGAACTGATCGACTGAACCTGTGATAGCGATACTCTGATTCACCGGCACATCGGCGAGGGCGCTTATCAGGGCGCAGAGTTCAGCCATCGA  >  NC_000913/1016718‑1016982

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑