Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,047,719	A→G	F76S (TTC→TCC)	gfcD ←	putative lipoprotein GfcD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,047,719	0	A	G	100.0%	241.3 / NA	68	F76S (TTC→TCC)	gfcD	putative lipoprotein GfcD
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (37/31);  total (37/31)

AGTAACCTTCTTCCCACAGGCGAAGTTTAAAATCGAATGATTTGTCTTTGTATGACTGATCGCCGCTGAAATCTTCCCACTGGCTATATTTGCGTGTGCGCACATCCGTATAACGAATGGTGCCTTCCAGCCATGGGAACAGCGCCACGGAGGTGGAGTAGAACCGGTATTGATCGTTATCCCGGTAGTTGACGCTGAATTCACCTTCCGGTGCGATGCGCGCATTTGGCATCTGCAACAATCCTGTGCCGCCAAAGTCTGATTGCGACGAAC  >  NC_000913/1047582‑1047854                                                                                                                                          |                                                                                                                                        aGTAACCTTCTTCCCACAGGCGAAGTTTAAAATCGAATGATTTGTCTTTGTATGACTGATCGCCGCTGAAATCTTCCCACTGGCTATATTTGCGTGTGCGCACATCCGTATAACGAATGGTGCCTTCCAGCCATGGGGAc                                                                                                                                       >  1:1282591/1‑140 (MQ=255) aGTAACCTTCTTCCCACAGGCGAAGTTTAAAATCGAATGATTTGTCTTTGTATGACTGATCGCCGCTGAAATCTTCCCACTGGCTATATTTGCGTGTGCGCACATCCGTATAACGAATGGTGCCTTCCAGCCATGGGGAc                                                                                                                                       >  1:789305/1‑140 (MQ=255)   tAACCTTCTTCCCACAGGCGAAGTTTAAAATCGAATGATTTGTCTTTGTATGACTGATCGCCGCTGAAATCTTCCCACTGGCTATATTTGCGTGTGCGCACATCCGTATAACGAATGGTGCCTTCCAGCCATGGGGACAg                                                                                                                                     <  1:728929/140‑1 (MQ=255)   tAACCTTCTTCCCACAGGCGAAGTTTAAAATCGAATGATTTGTCTTTGTATGACTGATCGCCGCTGAAATCTTCCCACTGGCTATATTTGCGTGTGCGCACATCCGTATAACGAATGGTGCCTTCCAGCCATGGGGACAg                                                                                                                                     <  2:612488/140‑1 (MQ=255)   tAACCTTCTTCCCACAGGCGAAGTTTAAAATCGAATGATTTGTCTTTGTATGACTGATCGCCGCTGAAATCTTCCCACTGGCTATATTTGCGTGTGCGCACATCCGTATAACGAATGGTGCCTTCCAGCCATGGGGACAg                                                                                                                                     >  2:156848/1‑140 (MQ=255)      ccTTCTTCCCACAGGCGAAGTTTAAAATCGAATGATTTGTCTTTGTATGACTGATCGCCGCTGAAATCTTCCCACTGGCTATATTTGCGTGTGCGCACATCCGTATAACGAATGGTGCCTTCCAGCCATGGGGACAgcgc                                                                                                                                  <  1:982652/140‑1 (MQ=255)        ttcttcCCACAGGCGAAGTTTAAAATCGAATGATTTGTCTTTGTATGACTGATCGCCGCTGAAATCTTCCCACTGGCTATATTTGCGTGTGCGCACATCCGTATAACGAATGGTGCCTTCCAGCCATGGGGACAGCGCCa                                                                                                                                <  2:1083585/140‑1 (MQ=255)           ttcCCACAGGCGAAGTTTAAAATCGAATGATTTGTCTTTGTATGACTGATCGCCGCTGAAATCTTCCCACTGGCTATATTTGCGTGTGCGCACATCCGTATAACGAATGGTGCCTTCCAGCCATGGGGACAGCGCCACgg                                                                                                                             <  2:789305/140‑1 (MQ=255)              ccACAGGCGAAGTTTAAAATCGAATGATTTGTCTTTGTATGACTGATCGCCGCTGAAATCTTCCCACTGGCTATATTTGCGTGTGCGCACATCCGTATAACGAATGGTGCCTTCCAGCCATGGGGACAGCGCCACGGAgg                                                                                                                          <  1:1080518/140‑1 (MQ=255)              ccACAGGCGAAGTTTAAAATCGAATGATTTGTCTTTGTATGACTGATCGCCGCTGAAATCTTCCCACTGGCTATATTTGCGTGTGCGCACATCCGTATAACGAATGGTGCCTTCCAGCCATGGGGACAGCGCCACGGAgg                                                                                                                          <  2:219950/140‑1 (MQ=255)                acaGGCGAAGTTTAAAATCGAATGATTTGTCTTTGTATGACTGATCGCCGCTGAAATCTTCCCACTGGCTATATTTGCGTGTGCGCACATCCGTATAACGAATGGTGCCTTCCAGCCATGGGGACAGCGCCACGGAGGTg                                                                                                                        >  2:49587/1‑140 (MQ=255)                 caGGCGAAGTTTAAAATCGAATGATTTGTCTTTGTATGACTGATCGCCGCTGAAATCTTCCCACTGGCTATATTTGCGTGTGCGCACATCCGTATAACGAATGGTGCCTTCCAGCCATGGGGACAGCGCCACGGAGGTgg                                                                                                                       >  1:959440/1‑140 (MQ=255)                 caGGCGAAGTTTAAAATCGAATGATTTGTCTTTGTATGACTGATCGCCGCTGAAATCTTCCCACTGGCTATATTTGCGTGTGCGCACATCCGTATAACGAATGGTGCCTTCCAGCCATGGGGACAGCGCCACGGAGGTgg                                                                                                                       >  1:808876/1‑140 (MQ=255)                    gCGAAGTTTAAAATCGAATGATTTGTCTTTGTATGACTGATCGCCGCTGAAATCTTCCCACTGGCTATATTTGCGTGTGCGCACATCCGTATAACGAATGGTGCCTTCCAGCCATGGGGACAGCGCCACGGAGGTGGAGt                                                                                                                    >  1:91563/1‑140 (MQ=255)                    gCGAAGTTTAAAATCGAATGATTTGTCTTTGTATGACTGATCGCCGCTGAAATCTTCCCACTGGCTATATTTGCGTGTGCGCACATCCGTATAACGAATGGTGCCTTCCAGCCATGGGGACAGCGCCACGGAGGTGGAGt                                                                                                                    <  1:619135/140‑1 (MQ=255)                        aGTTTAAAATCGAATGATTTGTCTTTGTATGACTGATCGCCGCTGAAATCTTCCCACTGGCTATATTTGCGTGTGCGCACATCCGTATAACGAATGGTGCCTTCCAGCCATGGGGACAGCGCCACGGAGGTGGAGTAGaa                                                                                                                <  1:414034/140‑1 (MQ=255)                          tttAAAATCGAATGATTTGTCTTTGTATGACTGATCGCCGCTGAAATCTTCCCACTGGCTATATTTGCGTGTGCGCACATCCGTATAACGAATGGTGCCTTCCAGCCATGGGGACAGCGCCACGGAGGTGGAGTAGAAcc                                                                                                              <  1:456048/140‑1 (MQ=255)                                aTCGAATGATTTGTCTTTGTATGACTGATCGCCGCTGAAATCTTCCCACTGGCTATATTTGCGTGTGCGCACATCCGTATAACGAATGGTGCCTTCCAGCCATGGGGACAGCGCCACGGAGGTGGAGTAGAACCGGTAtt                                                                                                        <  2:1282591/140‑1 (MQ=255)                                aTCGAATGATTTGTCTTTGTATGACTGATCGCCGCTGAAATCTTCCCACTGGCTATATTTGCGTGTGCGCACATCCGTATAACGAATGGTGCCTTCCAGCCATGGGGACAGCGCCACGGAGGTGGAGTAGAACCGGTAtt                                                                                                        <  2:499282/140‑1 (MQ=255)                                aTCGAATGATTTGTCTTTGTATGACTGATCGCCGCTGAAATCTTCCCACTGGCTATATTTGCGTGTGCGCACATCCGTATAACGAATGGTGCCTTCCAGCCATGGGGACAGCGCCACGGAGGTGGAGTAGAACCGGTAtt                                                                                                        <  2:91563/140‑1 (MQ=255)                                aTCGAATGATTTGTCTTTGTATGACTGATCGCCGCTGAAATCTTCCCACTGGCTATATTTGCGTGTGCGCACATCCGTATAACGAATGGTGCCTTCCAGCCATGGGGACAGCGCCACGGAGGTGGAGTAGAACCGGTAtt                                                                                                        <  1:347027/140‑1 (MQ=255)                                    aaTGATTTGTCTTTGTATGACTGATCGCCGCTGAAATCTTCCCACTGGCTATATTTGCGTGTGCGCACATCCGTATAACGAATGGTGCCTTCCAGCCATGGGGACAGCGCCACGGAGGTGGAGTAGAACCGGTATTGATc                                                                                                    >  2:181209/1‑140 (MQ=255)                                    aaTGATTTGTCTTTGTATGACTGATCGCCGCTGAAATCTTCCCACTGGCTATATTTGCGTGTGCGCACATCCGTATAACGAATGGTGCCTTCCAGCCATGGGGACAGCGCCACGGAGGTGGAGTAGAACCGGTATTGATc                                                                                                    >  2:324949/1‑140 (MQ=255)                                         tttGTCTTTGTATGACTGATCGCCGCTGAAATCTTCCCACTGGCTATATTTGCGTGTGCGCACATCCGTATAACGAATGGTGCCTTCCAGCCATGGGGACAGCGCCACGGAGGTGGAGTAGAACCGGTATTGATCGTTAt                                                                                               <  1:324949/140‑1 (MQ=255)                                                    aTGACTGATCGCCGCTGAAATCTTCCCACTGGCTATATTTGCGTGTGCGCACATCCGTATAACGAATGGTGCCTTCCAGCCATGGGGACAGCGCCACGGAGGTGGAGTAGAACCGGTATTGATCGTTATCCCGGTAGTTg                                                                                    <  2:262477/140‑1 (MQ=255)                                                       aCTGATCGCCGCTGAAATCTTCCCACTGGCTATATTTGCGTGTGCGCACATCCGTATAACGAATGGTGCCTTCCAGCCATGGGGACAGCGCCACGGAGGTGGAGTAGAACCGGTATTGATCGTTATCCCGGTAGTTGACg                                                                                 >  2:201764/1‑140 (MQ=255)                                                       aCTGATCGCCGCTGAAATCTTCCCACTGGCTATATTTGCGTGTGCGCACATCCGTATAACGAATGGTGCCTTCCAGCCATGGGGACAGCGCCACGGAGGTGGAGTAGAACCGGTATTGATCGTTATCCCGGTAGTTGACg                                                                                 >  1:83512/1‑140 (MQ=255)                                                           aTCGCCGCTGAAATCTTCCCACTGGCTATATTTGCGTGTGCGCACATCCGTATAACGAATGGTGCCTTCCAGCCATGGGGACAGCGCCACGGAGGTGGAGTAGAACCGGTATTGATCGTTATCCCGGTAGTTGACGCTGa                                                                             >  2:399368/1‑140 (MQ=255)                                                           aTCGCCGCTGAAATCTTCCCACTGGCTATATTTGCGTGTGCGCACATCCGTATAACGAATGGTGCCTTCCAGCCATGGGGACAGCGCCACGGAGGTGGAGTAGAACCGGTATTGATCGTTATCCCGGTAGTTGACGCTGa                                                                             >  2:159029/1‑140 (MQ=255)                                                           aTCGCCGCTGAAATCTTCCCACTGGCTATATTTGCGTGTGCGCACATCCGTATAACGAATGGTGCCTTCCAGCCATGGGGACAGCGCCACGGAGGTGGAGTAGAACCGGTATTGATCGTTATCCCGGTAGTTGACGCTGa                                                                             >  1:1384437/1‑140 (MQ=255)                                                           aTCGCCGCTGAAATCTTCCCACTGGCTATATTTGCGTGTGCGCACATCCGTATAACGAATGGTGCCTTCCAGCCATGGGGACAGCGCCACGGAGGTGGAGTAGAACCGGTATTGATCGTTATCCCGGTAGTTGACGCTGa                                                                             >  1:252372/1‑140 (MQ=255)                                                           aTCGCCGCTGAAATCTTCCCACTGGCTATATTTGCGTGTGCGCACATCCGTATAACGAATGGTGCCTTCCAGCCATGGGGACAGCGCCACGGAGGTGGAGTAGAACCGGTATTGATCGTTATCCCGGTAGTTGACGCTGa                                                                             >  1:1359926/1‑140 (MQ=255)                                                           aTCGCCGCTGAAATCTTCCCACTGGCTATATTTGCGTGTGCGCACATCCGTATAACGAATGGTGCCTTCCAGCCATGGGGACAGCGCCACGGAGGTGGAGTAGAACCGGTATTGATCGTTATCCCGGTAGTTGACGCTGa                                                                             >  2:850506/1‑140 (MQ=255)                                                           aTCGCCGCTGAAATCTTCCCACTGGCTATATTTGCGTGTGCGCACATCCGTATAACGAATGGTGCCTTCCAGCCATGGGGACAGCGCCACGGAGGTGGAGTAGAACCGGTATTGATCGTTATCCCGGTAGTTGACGCTGa                                                                             >  1:467979/1‑140 (MQ=255)                                                             cgccgcTGAAATCTTCCCACTGGCTATATTTGCGTGTGCGCACATCCGTATAACGAATGGTGCCTTCCAGCCATGGGGACAGCGCCACGGAGGTGGAGTAGAACCGGTATTGATCGTTATCCCGGTAGTTGACGCTGAAt                                                                           >  1:907335/1‑140 (MQ=255)                                                                  cTGAAATCTTCCCACTGGCTATATTTGCGTGTGCGCACATCCGTATAACGAATGGTGCCTTCCAGCCATGGGGACAGCGCCACGGAGGTGGAGTAGAACCGGTATTGATCGTTATCCCGGTAGTTGACGCTGAATTCAcc                                                                      <  2:1359926/140‑1 (MQ=255)                                                                               aCTGGCTATATTTGCGTGTGCGCACATCCGTATAACGAATGGTGCCTTCCAGCCATGGGGACAGCGCCACGGAGGTGGAGTAGAACCGGTATTGATCGTTATCCCGGTAGTTGACGCTGAATTCACCTTCCGGTGCGATg                                                         >  2:62577/1‑140 (MQ=255)                                                                                   gCTATATTTGCGTGTGCGCACATCCGTATAACGAATGGTGCCTTCCAGCCATGGGGACAGCGCCACGGAGGTGGAGTAGAACCGGTATTGATCGTTATCCCGGTAGTTGACGCTGAATTCACCTTCCGGTGCGATgcgcg                                                     <  1:949925/140‑1 (MQ=255)                                                                                    cTATATTTGCGTGTGCGCACATCCGTATAACGAATGGTGCCTTCCAGCCATGGGGACAGCGCCACGGAGGTGGAGTAGAACCGGTATTGATCGTTATCCCGGTAGTTGACGCTGAATTCACCTTCCGGTGCGATgcgcgc                                                    >  2:529085/1‑140 (MQ=255)                                                                                           tGCGTGTGCGCACATCCGTATAACGAATGGTGCCTTCCAGCCATGGGGACAGCGCCACGGAGGTGGAGTAGAACCGGTATTGATCGTTATCCCGGTAGTTGACGCTGAATTCACCTTCCGGTGCGATGCGCGCATTTGGc                                             <  2:252372/140‑1 (MQ=255)                                                                                           tGCGTGTGCGCACATCCGTATAACGAATGGTGCCTTCCAGCCATGGGGACAGCGCCACGGAGGTGGAGTAGAACCGGTATTGATCGTTATCCCGGTAGTTGACGCTGAATTCACCTTCCGGTGCGATGCGCGCATTTGGc                                             <  1:529085/140‑1 (MQ=255)                                                                                             cGTGTGCGCACATCCGTATAACGAATGGTGCCTTCCAGCCATGGGGACAGCGCCACGGAGGTGGAGTAGAACCGGTATTGATCGTTATCCCGGTAGTTGACGCTGAATTCACCTTCCGGTGCGATGCGCGCATTTGGCAt                                           >  1:225020/1‑140 (MQ=255)                                                                                              gtgtGCGCACATCCGTATAACGAATGGTGCCTTCCAGCCATGGGGACAGCGCCACGGAGGTGGAGTAGAACCGGTATTGATCGTTATCCCGGTAGTTGACGCTGAATTCACCTTCCGGTGCGATGCGCGCATTTGGCATc                                          <  1:1091495/140‑1 (MQ=255)                                                                                              gtgtGCGCACATCCGTATAACGAATGGTGCCTTCCAGCCATGGGGACAGCGCCACGGAGGTGGAGTAGAACCGGTATTGATCGTTATCCCGGTAGTTGACGCTGAATTCACCTTCCGGTGCGATGCGCGCATTTGGCATc                                          <  1:156848/140‑1 (MQ=255)                                                                                              gtgtGCGCACATCCGTATAACGAATGGTGCCTTCCAGCCATGGGGACAGCGCCACGGAGGTGGAGTAGAACCGGTATTGATCGTTATCCCGGTAGTTGACGCTGAATTCACCTTCCGGTGCGATGCGCGCATTTGGCATc                                          <  1:229872/140‑1 (MQ=255)                                                                                                 tgCGCACATCCGTATAACGAATGGTGCCTTCCAGCCATGGGGACAGCGCCACGGAGGTGGAGTAGAACCGGTATTGATCGTTATCCCGGTAGTTGACGCTGAATTCACCTTCCGGTGCGATGCGCGCATTTGGCATCTGc                                       >  1:896279/1‑140 (MQ=255)                                                                                                   cgcACATCCGTATAACGAATGGTGCCTTCCAGCCATGGGGACAGCGCCACGGAGGTGGAGTAGAACCGGTATTGATCGTTATCCCGGTAGTTGACGCTGAATTCACCTTCCGGTGCGATgcgcg                                                     <  2:240996/124‑1 (MQ=255)                                                                                                   cgcACATCCGTATAACGAATGGTGCCTTCCAGCCATGGGGACAGCGCCACGGAGGTGGAGTAGAACCGGTATTGATCGTTATCCCGGTAGTTGACGCTGAATTCACCTTCCGGTGCGATgcgcg                                                     >  1:240996/1‑124 (MQ=255)                                                                                                   cgcACATCCGTATAACGAATGGTGCCTTCCAGCCATGGGGACAGCGCCACGGAGGTGGAGTAGAACCGGTATTGATCGTTATCCCGGTAGTTGACGCTGAATTCACCTTCCGGTGCGATGCGCGCATTTGGCATCTGcaa                                     >  1:1288587/1‑140 (MQ=255)                                                                                                   cgcACATCCGTATAACGAATGGTGCCTTCCAGCCATGGGGACAGCGCCACGGAGGTGGAGTAGAACCGGTATTGATCGTTATCCCGGTAGTTGACGCTGAATTCACCTTCCGGTGCGATGCGCGCATTTGGCATCTGcaa                                     <  1:261833/140‑1 (MQ=255)                                                                                                   cgcACATCCGTATAACGAATGGTGCCTTCCAGCCATGGGGACAGCGCCACGGAGGTGGAGTAGAACCGGTATTGATCGTTATCCCGGTAGTTGACGCTGAATTCACCTTCCGGTGCGATGCGCGCATTTGGCATCTGcaa                                     <  1:181209/140‑1 (MQ=255)                                                                                                             tataACGAATGGTGCCTTCCAGCCATGGGGACAGCGCCACGGAGGTGGAGTAGAACCGGTATTGATCGTTATCCCGGTAGTTGACGCTGAATTCACCTTCCGGTGCGATGCGCGCATTTGGCATCTGCAACAATCCtgtg                           <  1:850506/140‑1 (MQ=255)                                                                                                               taACGAATGGTGCCTTCCAGCCATGGGGACAGCGCCACGGAGGTGGAGTAGAACCGGTATTGATCGTTATCCCGGTAGTTGACGCTGAATTCACCTTCCGGTGCGATGCGCGCATTTGGCATc                                          <  2:248489/123‑1 (MQ=255)                                                                                                               taACGAATGGTGCCTTCCAGCCATGGGGACAGCGCCACGGAGGTGGAGTAGAACCGGTATTGATCGTTATCCCGGTAGTTGACGCTGAATTCACCTTCCGGTGCGATGCGCGCATTTGGCATc                                          >  1:248489/1‑123 (MQ=255)                                                                                                                   gAATGGTGCCTTCCAGCCATGGGGACAGCGCCACGGAGGTGGAGTAGAACCGGTATTGATCGTTATCCCGGTAGTTGACGCTGAATTCACCTTCCGGTGCGATGCGCGCATTTGGCATCTGCAACAATCCTGTGCCGCCa                     <  1:698142/140‑1 (MQ=255)                                                                                                                    aaTGGTGCCTTCCAGCCATGGGGACAGCGCCACGGAGGTGGAGTAGAACCGGTATTGATCGTTATCCCGGTAGTTGACGCTGAATTCACCTTCCGGTGCGATGCGCGCATTTGGCATCTGCAACAATCCTGTGCCGCCaa                    >  2:1388256/1‑140 (MQ=255)                                                                                                                    aaTGGTGCCTTCCAGCCATGGGGACAGCGCCACGGAGGTGGAGTAGAACCGGTATTGATCGTTATCCCGGTAGTTGACGCTGAATTCACCTTCCGGTGCGATGCGCGCATTTGGCATCTGCAACAATCCTGTGCCGCCaa                    >  1:650760/1‑140 (MQ=255)                                                                                                                    aaTGGTGCCTTCCAGCCATGGGGACAGCGCCACGGAGGTGGAGTAGAACCGGTATTGATCGTTATCCCGGTAGTTGACGCTGAATTCACCTTCCGGTGCGATGCGCGCATTTGGCATCTGCAACAATCCTGTGCCGCCaa                    >  2:1007262/1‑140 (MQ=255)                                                                                                                    aaTGGTGCCTTCCAGCCATGGGGACAGCGCCACGGAGGTGGAGTAGAACCGGTATTGATCGTTATCCCGGTAGTTGACGCTGAATTCACCTTCCGGTGCGATGCGCGCATTTGGCATCTGCAACAATCCTGTGCCGCCaa                    >  2:200282/1‑140 (MQ=255)                                                                                                                    aaTGGTGCCTTCCAGCCATGGGGACAGCGCCACGGAGGTGGAGTAGAACCGGTATTGATCGTTATCCCGGTAGTTGACGCTGAATTCACCTTCCGGTGCGATGCGCGCATTTGGCATCTGCAACAATCCTGTGCCGCCaa                    >  2:1360228/1‑140 (MQ=255)                                                                                                                    aaTGGTGCCTTCCAGCAATGGGGACAGCGCCACGGAGGCGGAGTAGAACCGGTATTGATCGTTATCCAGGTAGTTGACGCTGAATTCACCTTCCGGTGCGATGCGCGCATTTGGCATCTGCAACAATCCTGTGCCGCCaa                    >  2:618097/1‑140 (MQ=255)                                                                                                                     aTGGTGCCTTCCAGCCATGGGGACAGCGCCACGGAGGTGGAGTAGAACCGGTATTGATCGTTATCCCGGTAGTTGACGCTGAATTCACCTTCCGGTGCGATGCGCGCATTTGGCATCTGCAACAATCCTGTGCCGCCaaa                   >  2:320587/1‑140 (MQ=255)                                                                                                                     aTGGTGCCTTCCAGCCATGGGGACAGCGCCACGGAGGTGGAGTAGAACCGGCATTGATCGTTATCCCGGCAGTTGCCGCTGAATTCACCTTCCGGTGCGATGCGCGCATTTGGCATCTGCAACAATCCTGTGCCGCCaaa                   >  2:166803/1‑140 (MQ=255)                                                                                                                        gTGCCTTCCAGCCATGGGGACAGCGCCACGGAGGTGGAGTAGAACCGGTATTGATCGTTATCCCGGTAGTTGACGCTGAATTCACCTTCCGGTGCGATGCGCGCATTTGGCATCTGCAACAATCCTGTGCCGCCAAAGTc                <  2:907335/140‑1 (MQ=255)                                                                                                                          gCCTTCCAGCCATGGGGACAGCGCCACGGAGGTGGAGTAGAACCGGTATTGATCGTTATCCCGGTAGTTGACGCTGAATTCACCTTCCGGTGCGATGCGCGCATTTGGCATCTGCAACAATCCTGTGCCGCCAAAGTCTg              >  1:166461/1‑140 (MQ=255)                                                                                                                                  gCCATGGGGACAGCGCCACGGAGGTGGAGTAGAACCGGTATTGATCGTTATCCCGGTAGTTGACGCTGAATTCACCTTCCGGTGCGATGCGCGCATTTGGCATCTGCAACAATCCTGTGCCGCCAAAGTCTGATTGcgac      <  1:1388256/140‑1 (MQ=255)                                                                                                                                  gCCATGGGGACAGCGCCACGGAGGTGGAGTAGAACCGGTATTGATCGTTATCCCGGTAGTTGACGCTGAATTCACCTTCCGGTGCGATGCGCGCATTTGGCATCTGCAACAATCCTGTGCCGCCAAAGTCTGATTGcgac      >  1:349439/1‑140 (MQ=255)                                                                                                                                      tGGGGACAGCGCCACGGAGGTGGAGTAGAACCGGTATTGATCGTTATCCCGGTAGTTGACGCTGAATTCACCTTCCGGTGCGATGCGCGCATTTGGCATCTGCAACAATCCTGTGCCGCCAAAGTCTGATTGCGACGAAc  >  1:860822/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                        AGTAACCTTCTTCCCACAGGCGAAGTTTAAAATCGAATGATTTGTCTTTGTATGACTGATCGCCGCTGAAATCTTCCCACTGGCTATATTTGCGTGTGCGCACATCCGTATAACGAATGGTGCCTTCCAGCCATGGGAACAGCGCCACGGAGGTGGAGTAGAACCGGTATTGATCGTTATCCCGGTAGTTGACGCTGAATTCACCTTCCGGTGCGATGCGCGCATTTGGCATCTGCAACAATCCTGTGCCGCCAAAGTCTGATTGCGACGAAC  >  NC_000913/1047582‑1047854

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑