Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,071,456	A→G	S104P (TCG→CCG)	rutD ←	putative aminoacrylate hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,071,456	0	A	G	100.0%	263.9 / NA	75	S104P (TCG→CCG)	rutD	putative aminoacrylate hydrolase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (40/35);  total (40/35)

GGAACAACGGCTGCGCTTCCACCCATGCCTGCGCGCCGCCGCTATACAGTAATCGTTCGCGAACCTGAAAACAGCGGCGCGTATGGGCGTTTATTCGTAGCCAGCCGTTAACGCTGATCAGCACAGTTACCGACGCGGGATAATCCAGCGCCAGCTGCATTCCCACCAGCGCACCGAGCGCATGGCCGACCACTGCGTAATGCTCAATCCCTGCGGCTACCAGCGCCTGATGCAGTTCCGCTGCCATCTGGGCGATACTGTAATCT  >  NC_000913/1071324‑1071589                                                                                                                                     |                                                                                                                                      ggAACAACGGCTGCGCTTCCACCCATGCCTGCGCGCCGCCGCTATACAGTAATCGTTCGCGAACCTGAAAACAGCGGCGCGTATGGGCGTTTATTCGTAGCCAGCCGTTAACGCTGATCAGCACAGTTACCGGCGCGGGa                                                                                                                                <  1:1072371/140‑1 (MQ=255)     caacGGCTGCGCTTCCACCCATGCCTGCGCGCCGCCGCTATACAGTAATCGTTCGCGAACCTGAAAACAGCGGCGCGTATGGGCGTTTATTCGTAGCCAGCCGTTAACGCTGATCAGCACAGTTACCGGCGCGGGATAAt                                                                                                                            >  2:1239913/1‑140 (MQ=255)     caacGGCTGCGCTTCCACCCATGCCTGCGCGCCGCCGCTATACAGTAATCGTTCGCGAACCTGAAAACAGCGGCGCGTATGGGCGTTTATTCGTAGCCAGCCGTTAACGCTGATCAGCACAGTTACCGGCGCGGGATAAt                                                                                                                            >  2:308441/1‑140 (MQ=255)          gCTGCGCTTCCACCCATGCCTGCGCGCCGCCGCTATACAGTAATCGTTCGCGAACCTGAAAACAGCGGCGCGTATGGGCGTTTATTCGTAGCCAGCCGTTAACGCTGATCAGCACAGTTACCGGcg                                                                                                                                     >  2:1361242/1‑126 (MQ=255)          gCTGCGCTTCCACCCATGCCTGCGCGCCGCCGCTATACAGTAATCGTTCGCGAACCTGAAAACAGCGGCGCGTATGGGCGTTTATTCGTAGCCAGCCGTTAACGCTGATCAGCACAGTTACCGGCGCGGGATAATCCAgc                                                                                                                       >  2:938116/1‑140 (MQ=255)            tGCGCTTCCACCCATGCCTGCGCGCCGCCGCTATACAGTAATCGTTCGCGAACCTGAAAACAGCGGCGCGTATGGGCGTTTATTCGTAGCCAGCCGTTAACGCTGATCAGCACAGTTACCGGCGCGGGATAATCCAgcgc                                                                                                                     <  2:929041/140‑1 (MQ=255)             gcgcTTCCACCCATGCCTGCGCGCCGCCGCTATACAGTAATCGTTCGCGAACCTGAAAACAGCGGCGCGTATGGGCGTTTATTCGTAGCCAGCCGTTAACGCTGATCAGCACAGTTACCGGCGCGGGATAATCCAGCGcc                                                                                                                    >  1:1396370/1‑140 (MQ=255)                    cACCCATGCCTGCGCGCCGCCGCTATACAGTAATCGTTCGCGAACCTGAAAACAGCGGCGCGTATGGGCGTTTATTCGTAGCCAGCCGTTAACGCTGATCAGCACAGTTACCGGCGCGGGATAATCCAGCGCCAGCTGCa                                                                                                             >  1:407027/1‑140 (MQ=255)                          tGCCTGCGCGCCGCCGCTATACAGTAATCGTTCGCGAACCTGAAAACAGCGGCGCGTATGGGCGTTTATTCGTAGCCAGCCGTTAACGCTGATCAGCACAGTTACCGGCGCGGGATAATCCAGCGCCAGCTGCATTCcca                                                                                                       >  2:349116/1‑140 (MQ=255)                           gCCTGCGCGCCGCCGCTATACAGTAATCGTTCGCGAACCTGAAAACAGCGGCGCGTATGGGCGTTTATTCGTAGCCAGCCGTTAACGCTGATCAGCACAGTTACCGGCGCGGGATAATCCAGCGCCAGCTGCATTCccac                                                                                                      >  2:282332/1‑140 (MQ=255)                              tGCGCGCCGCCGCTATACAGTAATCGTTCGCGAACCTGAAAACAGCGGCGCGTATGGGCGTTTATTCGTAGCCAGCCGTTAACGCTGATCAGCACAGTTACCGGCGCGGGATAATCCAGCGCCAGCTGCATTCCCACCAg                                                                                                   >  2:156816/1‑140 (MQ=255)                               gcgcgcCGCCGCTATACAGTAATCGTTCGCGAACCTGAAAACAGCGGCGCGTATGGGCGTTTATTCGTAGCCAGCCGTTAACGCTGATCAGCACAGTTACCGGCGCGGGATAATCCAGCGCCAGCTGCATTCCCACCAgc                                                                                                  >  2:989834/1‑140 (MQ=255)                                cgcgcCGCCGCTATACAGTAATCGTTCGCGAACCTGAAAACAGCGGCGCGTATGGGCGTTTATTCGTAGCCAGCCGTTAACGCTGATCAGCACAGTTACCGGCGCGGGATAATCCAGCGCCAGCTGCATTCCCACCAgcg                                                                                                 >  2:1092523/1‑140 (MQ=255)                                cgcgcCGCCGCTATACAGTAATCGTTCGCGAACCTGAAAACAGCGGCGCGTATGGGCGTTTATTCGTAGCCAGCCGTTAACGCTGATCAGCACAGTTACCGGCGCGGGATAATCCAGCGCCAGCTGCATTCCCACCAgcg                                                                                                 >  2:1003005/1‑140 (MQ=255)                                cgcgcCGCCGCTATACAGTAATCGTTCGCGAACCTGAAAACAGCGGCGCGTATGGGCGTTTATTCGTAGCCAGCCGTTAACGCTGATCAGCACAGTTACCGGCGCGGGATAATCCAGCGCCAGCTGCATTCCCACCAgcg                                                                                                 >  1:72421/1‑140 (MQ=255)                                    ccgccgcTATACAGTAATCGTTCGCGAACCTGAAAACAGCGGCGCGTATGGGCGTTTATTCGTAGCCAGCCGTTAACGCTGATCAGCACAGTTACCGGCGCGGGATAATCCAGCGCCAGCTGCATTCCCACCAGCGCAcc                                                                                             <  2:72421/140‑1 (MQ=255)                                    ccgccgcTATACAGTAATCGTTCGCGAACCTGAAAACAGCGGCGCGTATGGGCGTTTATTCGTAGCCAGCCGTTAACGCTGATCAGCACAGTTACCGGCGCGGGATAATCCAGCGCCAGCTGCATTCCCACCAGCGCAcc                                                                                             <  1:1003005/140‑1 (MQ=255)                                                aGTAATCGTTCGCGAACCTGAAAACAGCGGCGCGTATGGGCGTTTATTCGTAGCCAGCCGTTAACGCTGATCAGCACAGTTACCGGCGCGGGATAATCCAGCGCCAGCTGCATTCCCACCAGCGCACCGAGCGCATGGcc                                                                                 <  2:894555/140‑1 (MQ=255)                                                aGTAATCGTTCGCGAACCTGAAAACAGCGGCGCGTATGGGCGTTTATTCGTAGCCAGCCGTTAACGCTGATCAGCACAGTTACCGGCGCGGGATAATCCAGCGCCAGCTGCATTCCCACCAGCGCACCGAGCGCATGGcc                                                                                 <  1:1018597/140‑1 (MQ=255)                                                  tAATCGTTCGCGAACCTGAAAACAGCGGCGCGTATGGGCGTTTATTCGTAGCCAGCCGTTGACGCTGATCAGCACAGTTACCGGCGCGGGATAATCCAGCGCCAGCTGCATTCCCACCAGCGCACCGAGCGCa                                                                                      >  1:459224/1‑133 (MQ=255)                                                  tAATCGTTCGCGAACCTGAAAACAGCGGCGCGTATGGGCGTTTATTCGTAGCCAGCCGTTGACGCTGATCAGCACAGTTACCGGCGCGGGATAATCCAGCGCCAGCTGCATTCCCACCAGCGCACCGAGCGCa                                                                                      <  2:459224/133‑1 (MQ=255)                                                  tAATCGTTCGCGAACCTGAAAACAGCGGCGCGTATGGGCGTTTATTCGTAGCCAGCCGTTAACGCTGATCAGCACAGTTACCGGCGCGGGATAATCCAGCGCCAGCTGCATTCCCACCAGCGCACCGAGCGCATGGCCGa                                                                               <  1:349116/140‑1 (MQ=255)                                                  tAATCGTTCGCGAACCTGAAAACAGCGGCGCGTATGGGCGTTTATTCGTAGCCAGCCGTTAACGCTGATCAGCACAGTTACCGGCGCGGGATAATCCAGCGCCAGCTGCATTCCCACCAGCGCACCGAGCGCATGGCCGa                                                                               <  1:690570/140‑1 (MQ=255)                                                    aTCGTTCGCGAACCTGAAAACAGCGGCGCGTATGGGCGTTTATTCGTAGCCAGCCGTTAACGCTGATCAGCACAGTTACCGGCGCGGGATAATCCAGCGCCAGCTGCATTCCCACCAGCGCACCGAGCGCATGGCCGAcc                                                                             >  1:1189532/1‑140 (MQ=255)                                                    aTCGTTCGCGAACCTGAAAACAGCGGCGCGTATGGGCGTTTATTCGTAGCCAGCCGTTAACGCTGATCAGCACAGTTACCGGCGCGGGATAATCCAGCGCCAGCTGCATTCCCACCAGCGCACCGAGCGCATGGCCGAcc                                                                             >  2:860219/1‑140 (MQ=255)                                                    aTCGTTCGCGAACCTGAAAACAGCGGCGCGTATGGGCGTTTATTCGTAGCCAGCCGTTAACGCTGATCAGCACAGTTACCGGCGCGGGATAATCCAGCGCCAGCTGCATTCCCACCAGCGCACCGAGCGCATGGCCGAcc                                                                             >  1:811943/1‑140 (MQ=255)                                                     tCGTTCGCGAACCTGAAAACAGCGGCGCGTATGGGCGTTTATTCGTAGCCAGCCGTTAACGCTGATCAGCACAGTTACCGGCGCGGGATAATCCAGCGCCAGCTGCATTCCCACCAGCGCACCGAGCGCATGGCCGACCa                                                                            <  1:308441/140‑1 (MQ=255)                                                     tCGTTCGCGAACCTGAAAACAGCGGCGCGTATGGGCGTTTATTCGTAGCCAGCCGTTAACGCTGATCAGCACAGTTACCGGCGCGGGATAATCCAGCGCCAGCTGCATTCCCACCAGCGCACCGAGCGCATGGCCGACCa                                                                            <  1:156816/140‑1 (MQ=255)                                                       gTTCGCGAACCTGAAAACAGCGGCGCGTATGGGCGTTTATTCGTAGCCAGCCGTTAACGCTGATCAGCACAGTTACCGGCGCGGGATAATCCAGCGCCAGCTGCATTCCCACCAGCGCACCGAGCGCa                                                                                      <  1:831743/128‑1 (MQ=255)                                                       gTTCGCGAACCTGAAAACAGCGGCGCGTATGGGCGTTTATTCGTAGCCAGCCGTTAACGCTGATCAGCACAGTTACCGGCGCGGGATAATCCAGCGCCAGCTGCATTCCCACCAGCGCACCGAGCGCa                                                                                      >  2:831743/1‑128 (MQ=255)                                                       gTTCGCGAACCTGAAAACAGCGGCGCGTATGGGCGTTTATTCGTAGCCAGCCGTTAACGCTGATCAGCACAGTTACCGGCGCGGGATAATCCAGCGCCAGCTGCATTCCCACCAGCGCACCGAGCGCATGGc                                                                                  >  1:769853/1‑132 (MQ=255)                                                       gTTCGCGAACCTGAAAACAGCGGCGCGTATGGGCGTTTATTCGTAGCCAGCCGTTAACGCTGATCAGCACAGTTACCGGCGCGGGATAATCCAGCGCCAGCTGCATTCCCACCAGCGCACCGAGCGCATGGc                                                                                  <  2:769853/132‑1 (MQ=255)                                                       gTTCGCGAACCTGAAAACAGCGGCGCGTATGGGCGTTTATTCGTAGCCAGCCGTTAACGCTGATCAGCACAGTTACCGGCGCGGGATAATCCAGCGCCAGCTGCATTCCCACCAGCGCACCGAGCGCATGGCCGACCACt                                                                          >  1:1256479/1‑140 (MQ=255)                                                       gTTCGCGAACCTGAAAACAGCGGCGCGTATGGGCGTTTATTCGTAGCCAGCCGTTAACGCTGATCAGCACAGTTACCGGCGCGGGATAATCCAGCGCCAGCTGCATTCCCACCAGCGCACCGAGCGCATGGCCGACCACt                                                                          >  1:601311/1‑140 (MQ=255)                                                            cgAACCTGAAAACAGCGGCGCGTATGGGCGTTTATTCGTAGCCAGCCGTTAACGCTGATCAGCACAGTTACCGGCGCGGGATAATCCAGCGCCAGCTGCATTCCCACCAGCGCACCGAGCGCATGGCCGACCACTGCGTa                                                                     <  1:989834/140‑1 (MQ=255)                                                            cgAACCTGAAAACAGCGGCGCGTATGGGCGTTTATTCGTAGCCAGCCGTTAACGCTGATCAGCACAGTTACCGGCGCGGGATAATCCAGCGCCAGCTGCATTCCCACCAGCGCACCGAGCGCATGGCCGACCACTGCGTa                                                                     >  2:672986/1‑140 (MQ=255)                                                            cgAACCTGAAAACAGCGGCGCGTATGGGCGTTTATTCGTAGCCAGCCGTTAACGCTGATCAGCACAGTTACCGGCGCGGGATAATCCAGCGCCAGCTGCATTCCCACCAGCGCACCGAGCGCATGGCCGACCACTGCGTa                                                                     >  2:449062/1‑140 (MQ=255)                                                               aCCTGAAAACAGCGGCGCGTATGGGCGTTTATTCGTAGCCAGCCGTTAACGCTGATCAGCACAGTTACCGGCGCGGGATAATCCAGCGCCAGCTGCATTCCCACCAGCGCACCGAGCGCATGGCCGACCACTGCg                                                                       >  2:147243/1‑135 (MQ=255)                                                               aCCTGAAAACAGCGGCGCGTATGGGCGTTTATTCGTAGCCAGCCGTTAACGCTGATCAGCACAGTTACCGGCGCGGGATAATCCAGCGCCAGCTGCATTCCCACCAGCGCACCGAGCGCATGGCCGACCACTGCg                                                                       <  1:147243/135‑1 (MQ=255)                                                                    aaaaCAGCGGCGCGTATGGGCGTTTATTCGTAGCCAGCCGTTAACGCTGATCAGCACAGTTACCGGCGCGGGATAATCCAGCGCCAGCTGCATTCCCACCAGCGCACCGAGCGCATGGCCGACCACTGCGTAATGCTCaa                                                             <  2:586168/140‑1 (MQ=255)                                                                         aGCGGCGCGTATGGGCGTTTTTTCGTAGCCAGCCGTTAACGCTGATCAGCACAGTTACCGGCGCGGGATAATCCAGCGCCAGCTGCATTCCCACCAGCGCACCGAGCGCATGGCCGACCACTGCGTAATGCTCAATCCCt                                                        >  2:105668/1‑140 (MQ=255)                                                                         aGCGGCGCGTATGGGCGTTTATTCGTAGCCAGCCGTTAACGCTGATCAGCACAGTTACCGGCGCGGGATAATCCAGCGCCAGCTGCATTCCCACCAGCGCACCGAGCGCATGGCCGACCACTGCGTAATGCTCAATCCCt                                                        >  1:298498/1‑140 (MQ=255)                                                                           cggcgCGTATGGGCGTTTATTCGTAGCCAGCCGTTAACGCTGATCAGCACAGTTACCGGCGCGGGATAATCCAGCGCCAGCTGCATTCCCACCAGCGCACCGAGCGCATGGCCGACCACTGCGTAATGCTCAATCCCTGc                                                      >  2:538755/1‑140 (MQ=255)                                                                             gcgcGTATGGGCGTTTATTCGTAGCCAGCCGTTAACGCTGATCAGCACAGTTACCGGCGCGGGATAATCCAGCGCCAGCTGCATTCCCACCAGCGCACCGAGCGCATGGCCGACCACTGCGTAATGCTCAATCCCTGCgg                                                    <  2:1256479/140‑1 (MQ=255)                                                                                cgTATGGGCGTTTATTCGTAGCCAGCCGTTAACGCTGATCAGCACAGTTACCGGCGCGGGATAATCCAGCGCCAGCTGCATTCCCACCAGCGCACCGAGCGCATGGCCGACCACTGCGTAATGCTCAATCCCTGCGGCTa                                                 >  2:30641/1‑140 (MQ=255)                                                                                cgTATGGGCGTTTATTCGTAGCCAGCCGTTAACGCTGATCAGCACAGTTACCGGCGCGGGATAATCCAGCGCCAGCTGCATTCCCACCAGCGCACCGAGCGCATGGCCGACCACTGCGTAATGCTCAATCCCTGCGGCTa                                                 >  1:933669/1‑140 (MQ=255)                                                                                cgTATGGGCGTTTATTCGTAGCCAGCCGTTAACGCTGATCAGCACAGTTACCGGCGCGGGATAATCCAGCGCCAGCTGCATTCCCACCAGCGCACCGAGCGCATGGCCGACCACTGCGTAATGCTCAATCCCTGCGGCTa                                                 >  2:956344/1‑140 (MQ=255)                                                                                  tATGGGCGTTTATTCGTAGCCAGCCGTTAACGCTGATCAGCACAGTTACCGGCGCGGGATAATCCAGCGCCAGCTGCATTCCCACCAGCGCACCGAGCGCATGGCCGACCACTGCGTAATGCTCAATCCCTGCGGc                                                   >  2:212626/1‑136 (MQ=255)                                                                                  tATGGGCGTTTATTCGTAGCCAGCCGTTAACGCTGATCAGCACAGTTACCGGCGCGGGATAATCCAGCGCCAGCTGCATTCCCACCAGCGCACCGAGCGCATGGCCGACCACTGCGTAATGCTCAATCCCTGCGGc                                                   <  1:212626/136‑1 (MQ=255)                                                                                  tATGGGCGTTTATTCGTAGCCAGCCGTTAACGCTGATCAGCACAGTTACCGGCGCGGGATAATCCAGCGCCAGCTGCATTCCCACCAGCGCACCGAGCGCATGGCCGACCACTGCGTAATGCTCAATCCCTGCGGCTAcc                                               <  2:601311/140‑1 (MQ=255)                                                                                   aTGGGCGTTTATTCGTAGCCAGCCGTTAACGCTGATCAGCACAGTTACCGGCGCGGGATAATCCAGCGCCAGCTGCATTCCCACCAGCGCACCGAGCGCATGGCCGACCACTGCGTAATGCTCAATCCCTGCGGCTACCa                                              <  2:407027/140‑1 (MQ=255)                                                                                        cGTTTATTCGTAGCCAGCCGTTAACGCTGATCAGCACAGTTACCGGCGCGGGATAATCCAGCGCCAGCTGCATTCCCACCAGCGCACCGAGCGCATGGCCGACCACTGCGTAATGCTCAATCCCTGCGGCTACCAGCGcc                                         <  1:282332/140‑1 (MQ=255)                                                                                          tttATTCGTAGCCAGCCGTTAACGCTGATCAGCACAGTTACCGGCGCGGGATAATCCAGCGCCAGCTGCATTCCCACCAGCGCACCGAGCGCATGGCCGACCACTGCGTAATGCTCAATCCCTGCGGCTACCAGCGCCTg                                       >  1:854012/1‑140 (MQ=255)                                                                                             tttCGTAGCCAGCCGTTAACGCTGATCAGCACAGTTACCGGCGCGGGATAATCCAGCGCCAGCTGCATTCCCACCAGCGCACCGAGCGCATGGCCGACCACTGCGTAATGCTCAATCCCTGCGGCTACCAGCGCCTGATg                                    <  1:105668/139‑1 (MQ=255)                                                                                                  tAGCCAGCCGTTAACGCTGATCAGCACAGTTACCGGCGCGGGATAATCCAGCGCCAGCTGCATTCCCACCAGCGCACCGAGCGCATGGCCGACCACTGCGTAATGCTCAATCCCTGCGGCTACCAGCGCCTGATGCAGtt                               <  2:1396370/140‑1 (MQ=255)                                                                                                       agccGTTAACGCTGATCAGCACAGTTACCGGCGCGGGATAATCCAGCGCCAGCTGCATTCCCACCAGCGCACCGAGCGCATGGCCGACCACTGCGTAATGCTCAATCCCTGCGGCTACCAGCGCCTGATGCAGTTCCGCt                          <  1:1092523/140‑1 (MQ=255)                                                                                                         ccGTTAACGCTGATCAGCACAGTTACCGGCGCGGGATAATCCAGCGCCAGCTGCATTCCCACCAGCGCACCGAGCGCATGGCCGACCACTGCGTAATGCTCAATCCCTGCGGCTACCAGCGCCTGATGCAGTTCCGCt                          >  2:1272165/1‑138 (MQ=255)                                                                                                         ccGTTAACGCTGATCAGCACAGTTACCGGCGCGGGATAATCCAGCGCCAGCTGCATTCCCACCAGCGCACCGAGCGCATGGCCGACCACTGCGTAATGCTCAATCCCTGCGGCTACCAGCGCCTGATGCAGTTCCGCt                          <  1:1272165/138‑1 (MQ=255)                                                                                                            ttAACGCTGATCAGCACAGTTACCGGCGCGGGATAATCCAGCGCCAGCTGCATTCCCACCAGCGCACCGAGCGCATGGCCGACCACTGCGTAATGCTCAATCCCTGCGGCTACCAGCGCCTGATGCAGTTCCGCTGCCAt                     <  1:30641/140‑1 (MQ=255)                                                                                                            ttAACGCTGATCAGCACAGTTACCGGCGCGGGATAATCCAGCGCCAGCTGCATTCCCACCAGCGCACCGAGCGCATGGCCGACCACTGCGTAATGCTCAATCCCTGCGGCTACCAGCGCCTGATGCAGTTCCGCTGCCAt                     <  1:672986/140‑1 (MQ=255)                                                                                                            ttAACGCTGATCAGCACAGTTACCGGCGCGGGATAATCCAGCGCCAGCTGCATTCCCACCAGCGCACCGAGCGCATGGCCGACCACTGCGTAATGCTCAATCCCTGCGGCTACCAGCGCCTGATGCAGTTCCGCTGCCAt                     <  2:811943/140‑1 (MQ=255)                                                                                                             tAACGCTGATCAGCACAGTTACCGGCGCGGGATAATCCAGCGCCAGCTGCATTCCCACCAGCGCACCGAGCGCATGGCCGACCACTGCGTAATGCTCAATCCCTGCGGCTACCAGCGCCTGATGCAGTTCCGCTGCCATc                    >  1:957916/1‑140 (MQ=255)                                                                                                                 gCTGATCAGCACAGTCACCGGCGCGGGATAATCCAGCGCCAGCTGCATTCCCACCAGCGCACCGAGCGCATGGCCGACCACTGCGTAATGCTCAATCCCTGCGGCTACCAGCGCCTGATGCAGTTCCGCTGCCATCTggg                <  1:1333559/140‑1 (MQ=255)                                                                                                                   tGATCAGCACAGTTACCGGCGCGGGATAATCCAGCGCCAGCTGCATTCCCACCAGCGCACCGAGCGCATGGCCGACCACTGCGTAATGCTCAATCCCTGCGGCTACCAGCGCCTGATGCAGTTCCGCTGCCATCTGGGCg              <  2:803539/140‑1 (MQ=255)                                                                                                                    gATCAGCACAGTTACCGGCGCGGGATAATCCAGCGCCAGCTGCATTCCCACCAGCGCACCGAGCGCATGGCCGACCACTGCGTAATGCTCAATCCCTGCGGCTACCAGCGCCTGATGCAGTTCCGCTGCCATCTGGGCGa             <  2:1059933/140‑1 (MQ=255)                                                                                                                    gATCAGCACAGTTACCGGCGCGGGATAATCCAGCGCCAGCTGCATTCCCACCAGCGCACCGAGCGCATGGCCGACCACTGCGTAATGCTCAATCCCTGCGGCTACCAGCGCCTGATGCAGTTCCGCTGCCATCTGGGCGa             <  1:189726/140‑1 (MQ=255)                                                                                                                    gATCAGCACAGTTACCGGCGCGGGATAATCCAGCGCCAGCTGCATTCCCACCAGCGCACCGAGCGCATGGCCGACCACTGCGTAATGCTCAATCCCTGCGGCTACCAGCGCCTGATGCAGTTCCGCTGCCATCTGGGCGa             <  2:854012/140‑1 (MQ=255)                                                                                                                      tCAGCACAGTTACCGGCGCGGGATAATCCAGCGCCAGCTGCATTCCCACCAGCGCACCGAGCGCATGGCCGACCACTGCGTAATGCTCAATCCCTGCGGCTACCAGCGCCTGATGCAGTTCCGCTGCCATCTGGGCGATa           >  2:36129/1‑140 (MQ=255)                                                                                                                        aGCACAGTTACCGGCGCGGGATAATCCAGCGCCAGCTGCATTCCCACCAGCGCACCGAGCGCATGGCCGACCACTGCGTAATGCTCAATCCCTGCGGCTACCAGCGCCTGATGCAGTTCCGCTGCCATCTGGGCGATACt         <  1:1239913/140‑1 (MQ=255)                                                                                                                          cacaGTTACCGGCGCGGGATAATCCAGCGCCAGCTGCATTCCCACCAGCGCACCGAGCGCATGGCCGACCACTGCGTAATGCTCAATCCCTGCGGCTACCAGCGCCTGATGCAGTTCCGCTGCCATCTGGGCGATACTGt       >  2:673062/1‑140 (MQ=255)                                                                                                                          cacaGTTACCGGCGCGGGATAATCCAGCGCCAGCTGCATTCCCACCAGCGCACCGAGCGCATGGCCGACCACTGCGTAATGCTCAATCCCTGCGGCTACCAGCGCCTGATGCAGTTCCGCTGCCATCTGGGCGATACTGt       >  2:897380/1‑140 (MQ=255)                                                                                                                               ttACCGGCGCGGGATAATCCAGCGCCAGCTGCATTCCCACCAGCGCACCGAGCGCATGGCCGACCACTGCGTAATGCTCAATCCCTGCGGCTACCAGCGCCTGATGCAGTTCCGCTGCCATCTGGGCGATACTGTAAtct  >  1:445536/1‑140 (MQ=255)                                                                                                                               ttACCGGCGCGGGATAATCCAGCGCCAGCTGCATTCCCACCAGCGCACCGAGCGCATGGCCGACCACTGCGTAATGCTCAATCCCTGCGGCTACCAGCGCCTGATGCAGTTCCGCTGCCATCTGGGCGATACTGTAAtct  >  2:1083070/1‑140 (MQ=255)                                                                                                                               ttACCGGCGCGGGATAATCCAGCGCCAGCTGCATTCCCACCAGCGCACCGAGCGCATGGCCGACCACTGCGTAATGCTCAATCCCTGCGGCTACCAGCGCCTGATGCAGTTCCGCTGCCATCTGGGCGATACTGTAAtc   >  1:232398/1‑139 (MQ=255)                                                                                                                               ttACCGGCGCGGGATAATCCAGCGCCAGCTGCATTCCCACCAGCGCACCGAGCGCATGGCCGACCACTGCGTAATGCTCAATCCCTGCGGCTACCAGCGCCTGATGCAGTTCCGCTGCCATCTGGGCGATACTGTAAtc   <  2:232398/139‑1 (MQ=255)                                                                                                                                     |                                                                                                                                      GGAACAACGGCTGCGCTTCCACCCATGCCTGCGCGCCGCCGCTATACAGTAATCGTTCGCGAACCTGAAAACAGCGGCGCGTATGGGCGTTTATTCGTAGCCAGCCGTTAACGCTGATCAGCACAGTTACCGACGCGGGATAATCCAGCGCCAGCTGCATTCCCACCAGCGCACCGAGCGCATGGCCGACCACTGCGTAATGCTCAATCCCTGCGGCTACCAGCGCCTGATGCAGTTCCGCTGCCATCTGGGCGATACTGTAATCT  >  NC_000913/1071324‑1071589

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑