Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,131,290	C→T	D91D (GAC→GAT)	flgB →	flagellar basal‑body rod protein FlgB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,131,290	0	C	T	100.0%	155.3 / NA	44	D91D (GAC→GAT)	flgB	flagellar basal‑body rod protein FlgB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (17/27);  total (17/27)

GCCAGTGAACTTAAAAAAGTCATGCAACGTGGACGGGATGCAACCAGTGTGGTTGCACTGACGATGACCTCAACGCAACACATTCCGGCGCAGGCGCTGACGCCTCCTACCGCAGAACTGCAATACCGTATTCCGGACCAGCCTTCGCTTGACGGTAATACCGTCGATATGGATCGCGAACGCACCCAGTTTGCCGATAACAGCCTGCAATACCAGATGAGCCTTAGCGCGTTGAGCGGGCAAATCAAAGGCATGATGAACGTTTTACAG  >  NC_000913/1131153‑1131422                                                                                                                                          |                                                                                                                                     gCCAGTGAACTTAAAAAAGTCATGCAACGTGGACGGGATGCAACCAGTGTGGTTGCACTGACGATGACCTCAACGCAACACATTCCGGCGCAGGCGCTGACGCCTCCTACCGCAGAACTGCAATACCGTATTCCGGATCa                                                                                                                                    >  1:1013590/1‑140 (MQ=255)   cAGTGAACTTAAAAAAGTCATGCAACGTGGACGGGATGCAACCAGTGTGGTTGCACTGACGATGACCTCAACGCAACACATTCCGGCGCAGGCGCTGACGCCTCCTACCGCAGAACTGCAATACCGTATTCCGGATCAGc                                                                                                                                  <  2:463524/140‑1 (MQ=255)   cAGTGAACTTAAAAAAGTCATGCAACGTGGACGGGATGCAACCAGTGTGGTTGCACTGACGATGACCTCAACGCAACACATTCCGGCGCAGGCGCTGACGCCTCCTACCGCAGAACTGCAATACCGTATTCCGGATCAGc                                                                                                                                  <  2:329386/140‑1 (MQ=255)   cAGTGAACTTAAAAAAGTCATGCAACGTGGACGGGATGCAACCAGTGTGGTTGCACTGACGATGACCTCAACGCAACACATTCCGGCGCAGGCGCTGACGCCTCCTACCGCAGAACTGCAATACCGTATTCCGGATCAGc                                                                                                                                  <  1:1269269/140‑1 (MQ=255)         aCTTAAAAAAGTCATGCAACGTGGACGGGATGCAACCAGTGTGGTTGCACTGACGATGACCTCAACGCAACACATTCCGGCGCAGGCGCTGACGCCTCCTACCGCAGAACTGCAATACCGTATTCCGGATCAGCCTTCGc                                                                                                                            <  1:696351/140‑1 (MQ=255)           ttAAAAAAGTCATGCAACGTGGACGGGATGCAACCAGTGTGGTTGCACTGACGATGACCTCAACGCAACACATTCCGGCGCAGGCGCTGACGCCTCCTACCGCAGAACTGCAATACCGTATTCCGGATCAGCCTTCGCtt                                                                                                                          <  1:390020/140‑1 (MQ=255)                  aGTCATGCAACGTGGACGGGATGCAACCAGTGTGGTTGCACTGACGATGACCTCAACGCAACACATTCCGGCGCAGGCGCTGACGCCTCCTACCGCAGAACTGCAATACCGTATTCCGGATCAGCCTTCGCTTGACGGTa                                                                                                                   >  1:1173387/1‑140 (MQ=255)                    tCATGCAACGTGGACGGGATGCAACCAGTGTGGTTGCACTGACGATGACCTCAACGCAACACATTCCGGCGCAGGCGCTGACGCCTCCTACCGCAGAACTGCAATACCGTATTCCGGATCAGCCTTCGCTTGACGGTAAt                                                                                                                 >  2:1412548/1‑140 (MQ=255)                     cATGCAACGTGGACGGGATGCAACCAGTGTGGTTGCACTGACGATGACCTCAACGCAACACATTCCGGCGCAGGCGCTGACGCCTCCTACCGCAGAACTGCAATACCGTATTCCGGATCAGCCTTCGCtt                                                                                                                          >  2:96343/1‑130 (MQ=255)                     cATGCAACGTGGACGGGATGCAACCAGTGTGGTTGCACTGACGATGACCTCAACGCAACACATTCCGGCGCAGGCGCTGACGCCTCCTACCGCAGAACTGCAATACCGTATTCCGGATCAGCCTTCGCtt                                                                                                                          <  1:96343/130‑1 (MQ=255)                        gCAACGTGGACGGGATGCAACCAGTGTTGTTGCACTGACGATGACCTCAACGCAACACATTCCGGCGCAGGCGCTGACGCCTCCTACCGCAGAACTGCAATACCGTATTCCGGATCAGCCTTCGCTTGACGGTAATACCg                                                                                                             <  2:751735/140‑1 (MQ=255)                            cGTGGACGGGATGCAACCAGTGTGGTTGCACTGACGATGACCTCAACGCAACACATTCCGGCGCAGGCGCTGACGCCTCCTACCGCAGAACTGCAATACCGTATTCCGGATCAGCCTTCGCTTGACGGTAATACCGTCGa                                                                                                         <  1:669020/140‑1 (MQ=255)                                     gATGCAACCAGTGTGGTTGCACTGACGATGACCTCAACGCAACACATTCCGGCGCAGGCGCTGACGCCTCCTACCGCAGAACTGCAATACCGTATTCCGGATCAGCCTTCGCTTGACGGTAATACCGTCGATATGGATcg                                                                                                <  1:1319125/140‑1 (MQ=255)                                      aTGCAACCAGTGTGGTTGCACTGACGATGACCTCAACGCAACACATTCCGGCGCAGGCGCTGACGCCTCCTACCGCAGAACTGCAATACCGTATTCCGGATCAGCCTTCGCTTGACGGTAATACCGTCGATATGGATcgc                                                                                               >  2:321099/1‑140 (MQ=255)                                           aCCAGTGTGGTTGCACTGACGATGACCTCAACGCAACACATTCCGGCGCAGGCGCTGACGCCTCCTACCGCAGAACTGCAATACCGTATTCCGGATCAGCCTTCGCTTGACGGTAATACCGTCGATATGGATCGCGAACg                                                                                          <  1:353638/140‑1 (MQ=255)                                               gtgtGGTTGCACTGACGATGACCTCAACGCAACACATTCCGGCGCAGGCGCTGACGCCTCCTACCGCAGAACTGCAATACCGTATTCCGGATCAGCCTTCGCTTGACGGTAATACCGTCGATATGGATCGCGAACGCAcc                                                                                      >  1:227789/1‑140 (MQ=255)                                                   ggTTGCACTGACGATGACCTCAACGCAACACATTCCGGCGCAGGCGCTGACGCCTCCTACCGCAGAACTGCAATACCGTATTCCGGATCAGCCTTCGCTTGACGGTAATACCGTCGATATGGATCGCGAACGCACCCAGt                                                                                  <  1:1412548/140‑1 (MQ=255)                                                   ggTTGCACTGACGATGACCTCAACGCAACACATTCCGGCGCAGGCGCTGACGCCTCCTACCGCAGAACTGCAATACCGTATTCCGGATCAGCCTTCGCTTGACGGTAATACCGTCGATATGGATCGCGAACGCACCCAGt                                                                                  <  2:1173387/140‑1 (MQ=255)                                                   ggTTGCACTGACGATGACCTCAACGCAACACATTCCGGCGCAGGCGCTGACGCCTCCTACCGCAGAACTGCAATACCGTATTCCGGATCAGCCTTCGCTTGACGGTAATACCGTCGATATGGATCGCGAACGCACCCAGt                                                                                  <  2:382469/140‑1 (MQ=255)                                                    gTTGCACTGACGATGACCTCAACGCAACACATTCCGGCGCAGGCGCTGACGCCTCCTACCGCAGAACTGCAATACCGTATTCCGGATCAGCCTTCGCTTGACGGTAATACCGTCGATATGGATCGCGAACGCACCCAGtt                                                                                 >  2:1232690/1‑140 (MQ=255)                                                     ttGCACTGACGATGACCTCAACGCAACACATTCCGGCGCAGGCGCTGACGCCTCCTACCGCAGAACTGCAATACCGTATTCCGGATCAGCCTTCGCTTGACGGTAATACCGTCGATATGGATCGCGAACGCACCCAGttt                                                                                >  1:850664/1‑140 (MQ=255)                                                      tGCACTGACGATGACCTCAACGCAACACATTCCGGCGCAGGCGCTGACGCCTCCTACCGCAGAACTGCAATACCGTATTCCGGATCAGCCTTCGCTTGACGGTAATACCGTCGATATGGATCGCGAACGCACCCAGTTTg                                                                               >  2:361004/1‑140 (MQ=255)                                                       gCACTGACGATGACCTCAACGCAACACATTCCGGCGCAGGCGCTGACGCCTCCTACCGCAGAACTGCAATACCGTATTCCGGATCAGCCTTCGCTTGACGGTAATACCGTCGATATGGATCGCGAACGCACCCAGTTTGc                                                                              >  2:1151228/1‑140 (MQ=255)                                                          cTGACGATGACCTCAACGCAACACATTCCGGCGCAGGCGCTGACGCCTCCTACCGCAGAACTGCAATACCGTATTCCGGATCAGCCTTCGCTTGACGGTAATACCGTCGATATGGATCGCGAACGCACCCAGTTTGCCGa                                                                           >  2:491631/1‑140 (MQ=255)                                                          cTGACGATGACCTCAACGCAACACATTCCGGCGCAGGCGCTGACGCCTCCTACCGCAGAACTGCAATACCGTATTCCGGATCAGCCTTCGCTTGACGGTAATACCGTCGATATGGATCGCGAACGCACCCAGTTTGCCGa                                                                           >  1:204236/1‑140 (MQ=255)                                                               gATGACCTCAACGCAACACATTCCGGCGCAGGCGCTGACGCCTCCTACCGCAGAACTGCAATACCGTATTCCGGATCAGCCTTCGCTTGACGGTAATACCGTCGATATGGATCGCGAACGCACCCAGTTTGCCGATAACa                                                                      <  1:321099/140‑1 (MQ=255)                                                                aTGACCTCAACGCAACACATTCCGGCGCAGGCGCTGACGCCTCCTACCGCAGAACTGCAATACCGTATTCCGGATCAGCCTTCGCTTGACGGTAATACCGTCGATATGGATCGCGAACGCACCCAGTTTGCCGATAACAg                                                                     <  1:1076331/140‑1 (MQ=255)                                                                 tGACCTCAACGCAACACATTCCGGCGCAGGCGCTGACGCCTCCTACCGCAGAACTGCAATACCGTATTCCGGATCAGCCTTCGCTTGACGGTAATACCGTCGATATGGATCGCGAACGCACCCAGTTTGCCGATAACAGc                                                                    <  2:48511/140‑1 (MQ=255)                                                                  gACCTCAACGCAACACATTCCGGCGCAGGCGCTGACGCCTCCTACCGCAGAACTGCAATACCGTATTCCGGATCAGCCTTCGCTTGACGGTAATACCGTCGATATGGATCGCGAACGCACCCAGTTTGCCGATAACAGcc                                                                   >  2:422796/1‑140 (MQ=255)                                                                    ccTCAACGCAACACATTCCGGCGCAGGCGCTGACGCCTCCTACCGCAGAACTGCAATACCGTATTCCGGATCAGCCTTCGCTTGACGGTAATACCGTCGATATGGATCGCGAACGCACCCAGTTTGCCGATAACAGCCTg                                                                 <  2:187422/140‑1 (MQ=255)                                                                      tCAACGCAACACATTCCGGCGCAGGCGCTGACGCCTCCTACCGCAGAACTGCAATACCGTATTCCGGATCAGCCTTCGCTTGACGGTAATACCGTCGATATGGATCGCGAACGCACCCAGTTTGCCGATAACAGCCTGCa                                                               <  2:570909/140‑1 (MQ=255)                                                                       cAACGCAACACATTCCGGCGCAGGCGCTGACGCCTCCTACCGCAGAACTGCAATACCGTATTCCGGATCAGCCTTCGCTTGACGGTAATACCGTCGATATGGATCGCGAACGCACCCAGTTTGCCGATAACAGCCTGCaa                                                              >  2:1384200/1‑140 (MQ=255)                                                                         aCGCAACACATTCCGGCGCAGGCGCTGACGCCTCCTACCGCAGAACTGCAATACCGTATTCCGGATCAGCCTTCGCTTGACGGTAATACCGTCGATATGGATCGCGAACGCACCCAGTTTGCCGATAACAGCCTGCAATa                                                            <  2:1106716/140‑1 (MQ=255)                                                                         aCGCAACACATTCCGGCGCAGGCGCTGACGCCTCCTACCGCAGAACTGCAATACCGTATTCCGGATCAGCCTTCGCTTGACGGTAATACCGTCGATATGGATCGCGAACGCACCCAGTTTGCAGATAACAGCCTGCAATa                                                            <  1:1151228/140‑1 (MQ=255)                                                                               cacaTTCCGGCGCAGGCGCTGACGCCTCCTACCGCAGAACTGCAATACCGTATTCCGGATCAGCCTTCGCTTGACGGTAATACCGTCGATATGGATCGCGAACGCACCCAGTTTGCCGATAACAGCCTGCAATACCAGAt                                                      <  2:1013590/140‑1 (MQ=255)                                                                                         cgcAGGCGCTGACGCCTCCTACCGCAGAACTGCAATACCGTATTCCGGATCAGCCTTCGCTTGACGGTAATACCGTCGATATGGATCGCGAACGCACCCAGTTTGCCGATAACAGCCTGCAATACCAGATGAGCCTTAgc                                            >  1:1117711/1‑140 (MQ=255)                                                                                                gcTGACGCCTCCTACCGCAGAACTGCAATACCGTATTCCGGATCAGCCTTCGCTTGACGGTAATACCGTCGATATGGATCGCGAACGCACCCAGTTTGCCGATAACAGCCTGCAATACCAGATGAGCCTTAGCGCGTTGa                                     <  1:210493/140‑1 (MQ=255)                                                                                                gcTGACGCCTCCTACCGCAGAACTGCAATACCGTATTCCGGATCAGCCTTCGCTTGACGGTAATACCGTCGATATGGATCGCGAACGCACCCAGTTTGCCGATAACAGCCTGCAATACCAGATGAGCCTTAGCGCGTTGa                                     <  1:56598/140‑1 (MQ=255)                                                                                                 cTGACGCCTCCTACCGCAGAACTGCAATACCGTATTCCGGATCAGCCTTCGCTTGACGGTAATACCGTCGATATGGATCGCGAACGCACCCAGTTTGCCGATAACAGCCTGCAATACCAGATGAGCCTTAGCGCGTTGAg                                    <  1:230390/140‑1 (MQ=255)                                                                                                     cGCCTCCTACCGCAGAACTGCAATACCGTATTCCGGATCAGCCTTCGCTTGACGGTAATACCGTCGATATGGATCGCGAACGCACCCAGTTTGCCGATAACAGCCTGCAATACCAGATGAGCCTTAGCGCGTTGAGCggg                                <  1:676079/140‑1 (MQ=255)                                                                                                      gCCTCCTACCGCAGAACTGCAATACCGTATTCCGGATCAGCCTTCGCTTGACGGTAATACCGTCGATATGGATCGCGAACGCACCCAGTTTGCCGATAACAGCCTGCAATACCAGATGAGCCTTAGCGCGTTGAGCGGGc                               <  1:491631/140‑1 (MQ=255)                                                                                                                        gCAATACCGTATTCCGGATCAGCCTTCGCTTGACGGTAATACCGTCGATATGGATCGCGAACGCACCCAGTTTGCCGATAACAGCCTGCAATACCAGATGAGCCTTAGCGCGTTGAGCGGGCAAATCAAAGGCAtgatga             <  1:92603/140‑1 (MQ=255)                                                                                                                              ccGTATTCCTGATCAGCCTTCGCTTGACGGTAATACCGTCGATATGGATCGCGAACGCACCCAGTTTGCCGATAACAGCCTGCAATACCAGATGAGCCTTAGCGCGTTGAGCGGGCAAATCAAAGGCATGATGAACGttt       >  1:1042308/1‑140 (MQ=255)                                                                                                                                   ttCCGGATCAGCCTTCGCTTGACGGTAATACCGTCGATATGGATCGCGAACGCACCCAGTTTGCCGATAACAGCCTGCAATACCAGATGAGCCTTAGCGCGTTGAGCGGGCAAATCAAAGGCATGATGAACGTTTTACag  >  2:193380/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                     GCCAGTGAACTTAAAAAAGTCATGCAACGTGGACGGGATGCAACCAGTGTGGTTGCACTGACGATGACCTCAACGCAACACATTCCGGCGCAGGCGCTGACGCCTCCTACCGCAGAACTGCAATACCGTATTCCGGACCAGCCTTCGCTTGACGGTAATACCGTCGATATGGATCGCGAACGCACCCAGTTTGCCGATAACAGCCTGCAATACCAGATGAGCCTTAGCGCGTTGAGCGGGCAAATCAAAGGCATGATGAACGTTTTACAG  >  NC_000913/1131153‑1131422

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑