Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	164,230	T→C	V709A (GTG→GCG)	hrpB →	putative ATP‑dependent RNA helicase HrpB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	164,230	0	T	C	100.0%	280.0 / NA	79	V709A (GTG→GCG)	hrpB	putative ATP‑dependent RNA helicase HrpB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (41/38);  total (41/38)

GCCACATATGACTGGCGTACATTCACTACGCGGCCTGAAATCACTCGACATTTATCAGGCACTACGCGGATTACTTGATTGGGGAATGCAGCAACGTCTGGATAGTGAATTGCCTGCGCATTACACTGTGCCGACGGGAAGCCGGATCGCCATTCGTTATCATGAAGATAACCCGCCCGCGCTGGCGGTGAGAATGCAAGAGATGTTTGGCGAGGCCACCAATCCGACGATCGCCCAGGGGCGCGTGCCGCTGG  >  NC_000913/164102‑164355                                                                                                                                 |                                                                                                                              gcCACATATGACTGGCGTACATTCACTACGCGGCCTGAAATCACTCGACATTTATCAGGCACTACGCGGATTACTTGATTGGGGAATGCAGCAACGTCTGGATAGTGAATTGCCTGCGCATTACACTGCGCCGACGGGaa                                                                                                                    >  1:1357688/1‑140 (MQ=255)     caTATGACTGGCGTACATTCACTACGCGGCCTGAAATCACTCGACATTTATCAGGCACTACGCGGATTACTTGATTGGGGAATGCAGCAACGTCTGGATAGTGAATTGCCTGCGCATTACACTGCGCCGACGGGAAGCCg                                                                                                                <  1:581549/140‑1 (MQ=255)       tatGACTGGCGTACATTCACTACGCGGCCTGAAATCACTCGACATTTATCAGGCACTACGCGGATTACTTGATTGGGGAATGCAGCAACGTCTGGATAGTGAATTGCCTGCGCATTACACTGCGCCGACGGGAAGCCGGa                                                                                                              <  1:1293422/140‑1 (MQ=255)           aCTGGCGTACATTCACTACGCGGCCTGAAATCACTCGACATTTATCAGGCACTACGCGGATTACTTGATTGGGGAATGCAGCAGCGTCTGGATAGTGAATTGCCTGCGCATTACACTGCGCCGACGGGAAGCCGGATCGc                                                                                                          <  1:1371843/140‑1 (MQ=255)            cTGGCGTACATTCACTACGCGGCCTGAAATCACTCGACATTTATCAGGCACTACGCGGATTACTTGATTGGGGAATGCAGCAACGTCTGGATAGTGAATTGCCTGCGCATTACACTGCGCCGAc                                                                                                                         >  2:440579/1‑124 (MQ=255)            cTGGCGTACATTCACTACGCGGCCTGAAATCACTCGACATTTATCAGGCACTACGCGGATTACTTGATTGGGGAATGCAGCAACGTCTGGATAGTGAATTGCCTGCGCATTACACTGCGCCGAc                                                                                                                         <  1:440579/124‑1 (MQ=255)            cTGGCGTACATTCACTACGCGGCCTGAAATCACTCGACATTTATCAGGCACTACGCGGATTACTTGATTGGGGAATGCAGCAACGTCTGGATAGTGAATTGCCTGCGCATTACACTGCGCCGACGGGAAGCCGGATCGcc                                                                                                         <  2:837564/140‑1 (MQ=255)            cTGGCGTACATTCACTACGCGGCCTGAAATCACTCGACATTTATCAGGCACTACGCGGATTACTTGATTGGGGAATGCAGCAACGTCTGGATAGTGAATTGCCTGCGCATTACACTGCGCCGACGGGAAGCCGGATCGcc                                                                                                         >  1:904892/1‑140 (MQ=255)            cTGGCGTACATTCACTACGCGGCCTGAAATCACTCGACATTTATCAGGCACTACGCGGATTACTTGATTGGGGAATGCAGCAACGTCTGGATAGTGAATTGCCTGCGCATTACACTGCGCCGACGGGAAGCCGGATCGcc                                                                                                         >  1:1031398/1‑140 (MQ=255)            cTGGCGTACATTCACTACGCGGCCTGAAATCACTCGACATTTATCAGGCACTACGCGGATTACTTGATTGGGGAATGCAGCAACGTCTGGATAGTGAATTGCCTGCGCATTACACTGCGCCGACGGGAAGCCGGATCGcc                                                                                                         <  2:911531/140‑1 (MQ=255)               gCGTACATTCACTACGCGGCCTGAAATCACTCGACATTTATCAGGCACTACGCGGATTACTTGATTGGGGAATGCAGCAACGTCTGGATAGTGAATTGCCTGCGCATTACACTGCGCCGACGGGAAGCCGGATCGCCAtt                                                                                                      >  1:444624/1‑140 (MQ=255)                cGTACATTCACTACGCGGCCTGAAATCACTCGACATTTATCAGGCACTACGCGGATTACTTGATTGGGGAATGCAGCAACGTCTGGATAGTGAATTGCCTGCGCATTACACTGCGCCGACGGGAAGCCGGATCGCCATTc                                                                                                     >  2:1241229/1‑140 (MQ=255)                 gTACATTCACTACGCGGCCTGAAATCACTCGACATTTATCAGGCACTACGCGGATTACTTGATTGGGGAATGCAGCAACGTCTGGATAGTGAATTGCCTGCGCATTACACTGCGCCGACGGGAAGCCGGATCGCCATTCg                                                                                                    <  2:444624/140‑1 (MQ=255)                   aCATTCACTACGCGGCCTGAAATCACTCGACATTTATCAGGCACTACGCGGATTACTTGATTGGGGAATGCAGCAACGTCTGGATAGTGAATTGCCTGCGCATTACACTGCGCCGACGGGAAGCCGGATCGCCATTCGtt                                                                                                  <  2:551457/140‑1 (MQ=255)                   aCATTCACTACGCGGCCTGAAATCACTCGACATTTATCAGGCACTACGCGGATTACTTGATTGGGGAATGCAGCAACGTCTGGATAGTGAATTGCCTGCGCATTACACTGCGCCGACGGGAAGCCGGATCGCCATTCGtt                                                                                                  <  2:122978/140‑1 (MQ=255)                       tCACTACGCGGCCTGAAATCACTCGACATTTATCAGGCACTACGCGGATTACTTGATTGGGGAATGCAGCAACGTCTGGATAGTGAATTGCCTGCGCATTACACTGCGCCGACGGGAAGCCGGATCGCCATTCGTTATCa                                                                                              <  1:329627/140‑1 (MQ=255)                         aCTACGCGGCCTGAAATCACTCGACATTTATCAGGCACTACGCGGATTACTTGATTGGGGAATGCAGCAACGTCTGGATAGTGAATTGCCTGCGCATTACACTGCGCCGACGGGAAGCCGGATCGCCATTCGTTATCa                                                                                              <  2:319955/138‑1 (MQ=255)                         aCTACGCGGCCTGAAATCACTCGACATTTATCAGGCACTACGCGGATTACTTGATTGGGGAATGCAGCAACGTCTGGATAGTGAATTGCCTGCGCATTACACTGCGCCGACGGGAAGCCGGATCGCCATTCGTTATCa                                                                                              >  1:319955/1‑138 (MQ=255)                         aCTACGCGGCCTGAAATCACTCGACATTTATCAGGCACTACGCGGATTACTTGATTGGGGAATGCAGCAACGTCTGGATAGTGAATTGCCTGCGCATTACACTGCGCCGACGGGAAGCCGGATCGCCATTCGTTATCATg                                                                                            >  1:137095/1‑140 (MQ=255)                         aCTACGCGGCCTGAAATCACTCGACATTTATCAGGCACTACGCGGATTACTTGATTGGGGAATGCAGCAACGTCTGGATAGTGAATTGCCTGCGCATTACACTGCGCCGACGGGAAGCCGGATCGCCATTCGTTATCATg                                                                                            >  1:919644/1‑140 (MQ=255)                          cTACGCGGCCTGAAATCACTCGACATTTATCAGGCACTACGCGGATTACTTGATTGGGGAATGCAGCAACGTCTGGATAGTGAATTGCCTGCGCATTACACTGCGCCGACGGGAAGCCGGATCGCCATTCg                                                                                                    <  2:1167908/131‑1 (MQ=255)                          cTACGCGGCCTGAAATCACTCGACATTTATCAGGCACTACGCGGATTACTTGATTGGGGAATGCAGCAACGTCTGGATAGTGAATTGCCTGCGCATTACACTGCGCCGACGGGAAGCCGGATCGCCATTCg                                                                                                    >  1:1167908/1‑131 (MQ=255)                          cTACGCGGCCTGAAATCACTCGACATTTATCAGGCACTACGCGGATTACTTGATTGGGGAATGCAGCAACGTCTGGATAGTGAATTGCCTGCGCATTACACTGCGCCGACGGGAAGCCGGATCGCCATTCGTTATCAt                                                                                             <  1:1317354/138‑1 (MQ=255)                          cTACGCGGCCTGAAATCACTCGACATTTATCAGGCACTACGCGGATTACTTGATTGGGGAATGCAGCAACGTCTGGATAGTGAATTGCCTGCGCATTACACTGCGCCGACGGGAAGCCGGATCGCCATTCGTTATCAt                                                                                             >  2:1317354/1‑138 (MQ=255)                             cgcgGCCTGAAATCACTCGACATTTATCAGGCACTACGCGGATTACTTGATTGGGGAATGCAGCAACGTCTGGATAGTGAATTGCTTACGCATTACACTGCGCCGACGGGAAGCCGGATCGCCATTCGTTATCATGAAGa                                                                                        >  2:548799/1‑140 (MQ=255)                             cgcgGCCTGAAATCACTCGACATTTATCAGGCACTACGCGGATTACTTGATTGGGGAATGCAGCAACGTCTGGATAGTGAATTGCCTGCGCATTACACTGCGCCGACGGGAAGCCGGATCGCCATTCg                                                                                                    >  1:1035792/1‑128 (MQ=255)                             cgcgGCCTGAAATCACTCGACATTTATCAGGCACTACGCGGATTACTTGATTGGGGAATGCAGCAACGTCTGGATAGTGAATTGCCTGCGCATTACACTGCGCCGACGGGAAGCCGGATCGCCATTCg                                                                                                    <  2:1035792/128‑1 (MQ=255)                             cgcgGCCTGAAATCACTCGACATTTATCAGGCACTACGCGGATTACTTGATTGGGGAATGCAGCAACGTCTGGATAGTGAATTGCCTGCGCATTACACTGCGCCGACGGGAAGCCGGATCGCCATTCGTTATCATGAAGa                                                                                        >  2:1117995/1‑140 (MQ=255)                             cgcgGCCTGAAATCACTCGACATTTATCAGGCACTACGCGGATTACTTGATTGGGGAATGCAGCAACGTCTGGATAGTGAATTGCCTGCGCATTACACTGCGCCGACGGGAAGCCGGATCGCCATTCGTTATCATGAAGa                                                                                        >  1:999063/1‑140 (MQ=255)                             cgcgGCCTGAAATCACTCGACATTTATCAGGCACTACGCGGATTACTTGATTGGGGAATGCAGCAACGTCTGGATAGTGAATTGCCTGCGCATTACACTGCGCCGACGGGAAGCCGGATCGCCATTCGTTATCATGAAGa                                                                                        >  2:300458/1‑140 (MQ=255)                                 gCCTGAAATCACTCGACATTTATCAGGCACTACGCGGATTACTTGATTGGGGAATGCAGCAACGTCTGGATAGTGAATTGCCTGCGCATTACACTGCGCCGACGGGAAGCCGGATCGCCATTCGTTATCATGAAGATAAc                                                                                    >  2:1163748/1‑140 (MQ=255)                                  ccTGAAATCACTCGACATTTATCAGGCACTACGCGGATTACTTGATTGGGGAATGCAGCAACGTCTGGATAGTGAATTGCCTGCGCATTACACTGCGCCGACGGGAAGCCGGATCGCCATTCGTTATCATGAAGATAAcc                                                                                   >  2:1279660/1‑140 (MQ=255)                                      aaaTCACTCGACATTTATCAGGCACTACGCGGATTACTTGATTGGGGAATGCAGCAACGTCTGGATAGTGAATTGCCTGCGCATTACACTGCGCCGACGGGAAGCCGGATCGCCATTCGTTATCATGAAGATAAcccgcc                                                                               <  1:533103/140‑1 (MQ=255)                                          cACTCGACATTTATCAGGCACTACGCGGATTACTTGATTGGGGAATGCAGCAACGTCTGGATAGTGAATTGCCTGCGCATTACACTGCGCCGACGGGAAGCCGGATCGCCATTCGTTATCATGAAGATAACCCGCCcgcg                                                                           <  1:579136/140‑1 (MQ=255)                                          cACTCGACATTTATCAGGCACTACGCGGATTACTTGATTGGGGAATGCAGCAACGTCTGGATAGTGAATTGCCTGCGCATTACACTGCGCCGACGGGAAGCCGGATCGCCATTCGTTATCATGAAGATAACCCGCCcgcg                                                                           <  2:904892/140‑1 (MQ=255)                                          cACTCGACATTTATCAGGCACTACGCGGATTACTTGATTGGGGAATGCAGCAACGTCTGGATAGTGAATTGCCTGCGCATTACACTGCGCCGACGGGAAGCCGGATCGCCATTCGTTATCATGAAGATAACCCGCCcgcg                                                                           <  2:919644/140‑1 (MQ=255)                                            cTCGACATTTATCAGGCACTACGCGGATTACTTGATTGGGGAATGCAGCAACGTCTGGATAGTGAATTGCCTGCGCATTACACTGCGCCGACGGGAAGCCGGATCGCCATTCGTTATCATGAAGATAACCCGCCCGCGCt                                                                         >  2:1035081/1‑140 (MQ=255)                                                aCATTTATCAGGCACTACGCGGATTACTTGATTGGGGAATGCAGCAACGTCTGGATAGTGAATTGCCTGCGCATTACACTGCGCCGACGGGAAGCCGGATCGCCATTCGTTATCATGAAGATAACCCGCCCGCGCTGGCg                                                                     >  2:1267555/1‑140 (MQ=255)                                                aCATTTATCAGGCACTACGCGGATTACTTGATTGGGGAATGCAGCAACGTCTGGATAGTGAATTGCCTGCGCATTACACTGCGCCGACGGGAAGCCGGATCGCCATTCGTTATCATGAAGATAACCCGCCCGCGCTGGCg                                                                     >  1:216745/1‑140 (MQ=255)                                                    ttATCAGGCACTACGCGGATTACTTGATTGGGGAATGCAGCAACGTCTGGATAGTGAATTGCCTGCGCATTACACTGCGCCGACGGGAAGCCGGATCGCCATTCGTTATCATGAAGATAACCCGCCCGCGCTGGCGGTga                                                                 <  1:1061281/140‑1 (MQ=255)                                                     tATCAGGCACTACGCGGATTACTTGATTGGGGAATGCAGCAACGTCTGGATAGTGAATTGCCTGCGCATTACACTGCGCCGACGGGAAGCCGGATCGCCATTCGTTATCATGAAGATAACCCGCCCGCGCTGGCGGTgag                                                                <  2:1160775/140‑1 (MQ=255)                                                           gCACTACGCGGATTACTTGATTGGGGAATGCAGCAACGTCTGGATAGTGAATTGCCTGCGCATTACACTGCGCCGACGGGAAGCCGGATCGCCATTCGTTATCATGAAGATAACCCGCCCGCGCTGGCGGTGAGAATGCa                                                          >  1:952410/1‑140 (MQ=255)                                                           gCACTACGCGGATTACTTGATTGGGGAATGCAGCAACGTCTGGATAGTGAATTGCCTGCGCATTACACTGCGCCGACGGGAAGCCGGATCGCCATTCGTTATCATGAAGATAACCCGCCCGCGCTGGCGGTGAGAATGCa                                                          >  1:495350/1‑140 (MQ=255)                                                              cTACGCGGATTACTTGATTGGGGAATGCAGCAACGTCTGGATAGTGAATTGCCTGCGCATTACACTGCGCCGACGGGAAGCCGGATCGCCATTCGTTATCATGAAGATAACCCGCCCGCGCTGGCGGTGAGAATGCAaga                                                       >  1:254957/1‑140 (MQ=255)                                                               tACGCGGATTACTTGATTGGGGAATGCAGCAACGTCTGGATAGTGAATTGCCTGCGCATTACACTGCGCCGACGGGAAGCCGGATCGCCATTCGTTATCATGAAGATAACCCGCCCGCGCTGGCGGTGAGAATGCAagag                                                      >  1:1155501/1‑140 (MQ=255)                                                               tACGCGGATTACTTGATTGGGGAATGCAGCAACGTCTGGATAGTGAATTGCCTGCGCATTACACTGCGCCGACGGGAAGCCGGATCGCCATTCGTTATCATGAAGATAACCCGCCCGCGCTGGCGGTGAGAATGCAagag                                                      >  1:709030/1‑140 (MQ=255)                                                                 cgcgGATTACTTGATTGGGGAATGCAGCAACGTCTGGATAGTGAATTGCCTGCGCATTACACTGCGCCGACGGGAAGCCGGATCGCCATTCGTTATCATGAAGATAACCCGCCCGCGCTGGCGGTGAGAATGCAagag                                                      >  2:762985/1‑138 (MQ=255)                                                                 cgcgGATTACTTGATTGGGGAATGCAGCAACGTCTGGATAGTGAATTGCCTGCGCATTACACTGCGCCGACGGGAAGCCGGATCGCCATTCGTTATCATGAAGATAACCCGCCCGCGCTGGCGGTGAGAATGCAagag                                                      <  1:762985/138‑1 (MQ=255)                                                                 cgcgGATTACTTGATTGGGGAATGCAGCAACGTCTGGATAGTGAATTGCCTGCGCATTACACTGCGCCGACGGGAAGCCGGATCGCCATTCGTTATCATGAAGATAACCCGCCCGCGCTGGCGGTGAGAATGCAAGAGAt                                                    >  2:432950/1‑140 (MQ=255)                                                                         aCTTGATTGGGGAATGCAGCAACGTCTGGATAGTGAATTGCCTGCGCATTACACTGCGCCGACGGGAAGCCGGATCGCCATTCGTTATCATGAAGATAACCCGCCCGCGCTGGCGGTGAGAATGCAAGAGATGTTTGGCg                                            >  2:681373/1‑140 (MQ=255)                                                                            tGATTGGGGAATGCAGCAACGTCTGGATAGTGAATTGCCTGCGCATTACACTGCGCCGACGGGAAGCCGGATCGCCATTCGTTATCATGAAGATAACCCGCCCGCGCTGGCGGTGAGAATGCAAGAGATGTTTGGCGAgg                                         >  2:556637/1‑140 (MQ=255)                                                                             gATTGGGGAATGCAGCAACGTCTGGATAGTGAATTGCCTGCGCATTACACTGCGCCGACGGGAAGCCGGATCGCCATTCGTTATCATGAAGATAACCCGCCCGCGCTGGCGGTGAGAATGCAAGAGATGTTTGGCGAGGc                                        <  2:709030/140‑1 (MQ=255)                                                                               ttGGGGAATGCAGCAACGTCTGGATAGTGAATTGCCTGCGCATTACACTGCGCCGACGGGAAGCCGGATCGCCATTCGTTATCATGAAGATAACCCGCCCGCGCTGGCGGTGAGAATGCAAGAGATGTTTGGCGAGGcca                                      <  2:495350/140‑1 (MQ=255)                                                                               ttGGGGAATGCAGCAACGTCTGGATAGTGAATTGCCTGCGCATTACACTGCGCCGACGGGAAGCCGGATCGCCATTCGTTATCATGAAGATAACCCGCCCGCGCTGGCGGTGAGAATGCAAGAGATGTTTGGCGAGGcca                                      <  2:909563/140‑1 (MQ=255)                                                                                tGGGGAATGCAGCAACGTCTGGATAGTGAATTGCCTGCGCATTACACTGCGCCGACGGGAAGCCGGATCGCCATTCGTTATCATGAAGATAACCCGCCCGCGCTGGCGGTGAGAATGCAAGAGATGTTTGGCGAGGccac                                     >  2:814908/1‑140 (MQ=255)                                                                                  gggAATGCAGCAACGTCTGGATAGTGAATTGCCTGCGCATTACACTGCGCCGACGGGAAGCCGGATCGCCATTCGTTATCATGAAGATAACCCGCCCGCGCTGGCGGTGAGAATGCAAGAGATGTTTGGCGAGGccacca                                   >  1:1186464/1‑140 (MQ=255)                                                                                      aTGCAGCAACGTCTGGATAGTGAATTGCCTGCGCATTACACTGCGCCGACGGGAAGCCGGATCGCCATTCGTTATCATGAAGATAACCCGCCCGCGCTGGCGGTGAGAATGCAAGAGATGTTTGGCGAGGCCACCAATcc                               <  1:1288180/140‑1 (MQ=255)                                                                                       tGCAGCAACGTCTGGATAGTGAATTGCCTGCGCATTACACTGCGCCGACGGGAAGCCGGATCGCCATTCGTTATCATGAAGATAACCCGCCCGCGCTGGCGGTGAGAATGCAAGAGATGTTTGGCGAGGCCACCAATCcg                              >  2:1265126/1‑140 (MQ=255)                                                                                       tGCAGCAACGTCTGGATAGTGAATTGCCTGCGCATTACACTGCGCCGACGGGAAGCCGGATCGCCATTCGTTATCATGAAGATAACCCGCCCGCGCTGGCGGTGAGAATGCAAGAGATGTTTGGCGAGGCCACCAATCcg                              >  1:419058/1‑140 (MQ=255)                                                                                           gcaACGTCTGGATAGTGAATTGCCTGCGCATTACACTGCGCCGACGGGAAGCCGGATCGCCATTCGTTATCATGAAGATAACCCGCCCGCGCTGGCGGTGAGAATGCAAGAGATGTTTGGCGAGGCCACCAATCcgacga                          <  2:952410/140‑1 (MQ=255)                                                                                           gcaACGTCTGGATAGTGAATTGCCTGCGCATTACACTGCGCCGACGGGAAGCCGGATCGCCATTCGTTATCATGAAGATAACCCGCCCGCGCTGGCGGTGAGAATGCAAGAGATGTTTGGCGAGGCCACCAATCcgacga                          <  2:216745/140‑1 (MQ=255)                                                                                           gcaACGTCTGGATAGTGAATTGCCTGCGCATTACACTGCGCCGACGGGAAGCCGGATCGCCATTCGTTATCATGAAGATAACCCGCCCGCGCTGGCGGTGAGAATGCAAGAGATGTTTGGCGAGGCCACCAATCcgacga                          <  1:300458/140‑1 (MQ=255)                                                                                           gcaACGTCTGGATAGTGAATTGCCTGCGCATTACACTGCGCCGACGGGAAGCCGGATCGCCATTCGTTATCATGAAGATAACCCGCCCGCGCTGGCGGTGAGAATGCAAGAGATGTTTGGCGAGGCCACCAATCcgacga                          <  2:137095/140‑1 (MQ=255)                                                                                           gcaACGTCTGGATAGTGAATTGCCTGCGCATTACACTGCGCCGACGGGAAGCCGGATCGCCATTCGTTATCATGAAGATAACCCGCCCGCGCTGGCGGTGAGAATGCAAGAGATGTTTGGCGAGGCCACCAATCcgacga                          <  1:959270/140‑1 (MQ=255)                                                                                            caACGTCTGGATAGTGAATTGCCTGCGCATTACACTGCGCCGACGGGAAGCCGGATCGCCATTCGTTATCATGAAGATAACCCGCCCGCGCTGGCGGTGAGAATTCAAGAGATGTTTGGCGAGGCCACCAATCCGACGAt                         >  1:354228/1‑140 (MQ=255)                                                                                            caACGTCTGGATAGTGAATTGCCTGCGCATTACACTGCGCCGACGGGAAGCCGGATCGCCATTCGTTATCATGAAGATAACCCGCCCGCGCTGGCGGTGAGAATGCAAGAGATGTTTGGCGAGGCCACCAATcc                               <  1:653044/134‑1 (MQ=255)                                                                                            caACGTCTGGATAGTGAATTGCCTGCGCATTACACTGCGCCGACGGGAAGCCGGATCGCCATTCGTTATCATGAAGATAACCCGCCCGCGCTGGCGGTGAGAATGCAAGAGATGTTTGGCGAGGCCACCAATcc                               >  2:653044/1‑134 (MQ=255)                                                                                             aaCGTCTGGATAGTGAATTGCCTGCGCATTACACTGCGCCGACGGGAAGCCGGATCGCCATTCGTTATCATGAAGATAACCCGCCCGCGCTGGCGGTGAGAATGCAAGAGATGTTTGGCGATGCCACCAATCCGACGATc                        >  1:79627/1‑140 (MQ=255)                                                                                              aCGTCTGGATAGTGAATTGCCTGCGCATTACACTGCGCCGACGGGAAGCCGGATCGCCATTCGTTATCATGAAGATAACCCGCCCGCGCTGGCGGTGAGAATGCAAGAGATGTTTGGCGAGGCCACCAATCCGACGATCg                       >  2:190855/1‑140 (MQ=255)                                                                                                 tCTGGATAGTGAATTGCCTGCGCATTACACTGCGCCGACGGGAAGCCGGATCGCCATTCGTTATCATGAAGATAACCCGCCCGCGCTGGCGGTGAGAATGCAAGAGATGTTTGGCGAGGCCACCAATCCGACGATCGccc                    >  2:1208143/1‑140 (MQ=255)                                                                                                      aTAGTGAATTGCCTGCGCATTACACTGCGCCGACGGGAAGCCGGATCGCCATTCGTTATCATGAAGATAACCCGCCCGCGCTGGCGGTGAGAATGCAAGAGATGTTTGGCGAGGCCACCAATCCGACGATCGCCCAgggg               <  1:1117995/140‑1 (MQ=255)                                                                                                               tGCCTGCGCATTACACTGCGCCGACGGGAAGCCGGATCGCCATTCGTTATCATGAAGATAACCCGCCCGCGCTGGCGGTGAGAATGCAAGAGATGTTTGGCGAGGCCACCAATCCGACGATCGCCCAGGGGCGCGTGCCg      <  1:1163748/140‑1 (MQ=255)                                                                                                               tGCCTGCGCATTACACTGCGCCGACGGGAAGCCGGATCGCCATTCGTTATCATGAAGATAACCCGCCCGCGCTGGCGGGGAGAACGCAAGAAGTGTTTGGCGGGGCCACCAATCCGACGATCGCCCAGGGGCGGGCGccc      >  2:154960/1‑139 (MQ=255)                                                                                                                gCCTGCGCATTACACTGCGCCGACGGGAAGCCGGATCGCCATTCGTTATCATGAAGATAACCCGCCCGCGCTGGCGGTGAGAATGCAAGAGATGTTTGGCGATGCCACCAATCCGACGATCGCCCAGGGGCGCGTGCCGc     <  2:79627/140‑1 (MQ=255)                                                                                                                gCCTGCGCATTACACTGCGCCGACGGGAAGCCGGATCGCCATTCGTTATCATGAAGATAACCCGCCCGCGCTGGCGGTGAGAATGCAAGAGATGTTTGGCGAGGCCACCAATCCGACGATCGCCCAGGGGCGCGTGCCGc     <  1:814908/140‑1 (MQ=255)                                                                                                                gCCTGCGCATTACACTGCGCCGACGGGAAGCCGGATCGCCATTCGTTATCATGAAGATAACCCGCCCGCGCTGGCGGTGAGAATGCAAGAGATGTTTGGCGAGGCCACCAATCCGACGATCGCCCAGGGGCGCGTGCCGc     <  2:1357688/140‑1 (MQ=255)                                                                                                                gCCTGCGCATTACACTGCGCCGACGGGAAGCCGGATCGCCATTCGTTATCATGAAGATAACCCGCCCGCGCTGGCGGTGAGAATGCAAGAGATGTTTGGCGAGGCCACCAATCCGACGATCGCCCAGGGGCGCGTGCCGc     <  2:16716/140‑1 (MQ=255)                                                                                                                   tGCGCATTACACTGCGCCGACGGGAAGCCGGATCGCCATTCGTTATCATGAAGATAACCCGCCCGCGCTGGCGGTGAGAATGCAAGAGATGTTTGGCGAGGCCACCAATCCGACGATCGCCCAGGGGCGCGTGCCGCTgg  <  2:1086575/140‑1 (MQ=255)                                                                                                                   tGCGCATTACACTGCGCCGACGGGAAGCCGGATCGCCATTCGTTATCATGAAGATAACCCGCCCGCGCTGGCGGTGAGAATGCAAGAGATGTTTGGCGAGGCCACCAATCCGACGATCGCCCAGGGGCGCGTGCCGCTgg  >  2:881422/1‑140 (MQ=255)                                                                                                                                 |                                                                                                                              GCCACATATGACTGGCGTACATTCACTACGCGGCCTGAAATCACTCGACATTTATCAGGCACTACGCGGATTACTTGATTGGGGAATGCAGCAACGTCTGGATAGTGAATTGCCTGCGCATTACACTGTGCCGACGGGAAGCCGGATCGCCATTCGTTATCATGAAGATAACCCGCCCGCGCTGGCGGTGAGAATGCAAGAGATGTTTGGCGAGGCCACCAATCCGACGATCGCCCAGGGGCGCGTGCCGCTGG  >  NC_000913/164102‑164355

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑