Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,283,009	A→G	H1049R (CAC→CGC)	narG →	nitrate reductase A subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,283,009	0	A	G	100.0%	210.5 / NA	60	H1049R (CAC→CGC)	narG	nitrate reductase A subunit alpha
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (34/26);  total (34/26)

GCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGACACCCGTTCGGTGAAAGAAGTGATAGGCCAGAAATCCAACGGCAACCAGGAAAAAGCGCTCAACTTCCTGACGC  >  NC_000913/1282872‑1283137                                                                                                                                          |                                                                                                                                 gCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCGcc                                                                                                                                >  2:1089616/1‑140 (MQ=255) gCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCGcc                                                                                                                                >  1:675967/1‑140 (MQ=255) gCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCGcc                                                                                                                                >  1:1270324/1‑140 (MQ=255)     cgcAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCGCCAGTg                                                                                                                            >  1:70503/1‑140 (MQ=255)     cgcAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCGCCAGTg                                                                                                                            >  1:605914/1‑140 (MQ=255)      gcAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCGCCAGTgg                                                                                                                           >  2:79344/1‑140 (MQ=255)                ctcCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCGCCAGTGGATGCGTGAtt                                                                                                                 >  1:528620/1‑140 (MQ=255)                 tcCAGCCCGACCTGGTCTGGTCTGGAAGATGAACTCGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCGCTCTGGTCGTCAGCAACTGTATCAGGATCGCCAGTGGATGCGTGAttt                                                                                                                <  1:712386/140‑1 (MQ=255)                 tcCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCGCCAGTGGATGCGTGAttt                                                                                                                <  1:327938/140‑1 (MQ=255)                 tcCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCGCCAGTGGATGCGTGAttt                                                                                                                <  2:528620/140‑1 (MQ=255)                 tcCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCGCCAGTGGATGCGTGAttt                                                                                                                <  1:1065594/140‑1 (MQ=255)                 tcCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCGCCAGTGGATGCGTGAttt                                                                                                                <  1:1249725/140‑1 (MQ=255)                    aGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCGCCAGTGGATGCGTGATTTCgg                                                                                                             <  2:605914/140‑1 (MQ=255)                      cccGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCGCCAGTGGATGCGTGATTTCGGTg                                                                                                           >  1:880979/1‑140 (MQ=255)                      cccGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCGCCAGTGGATGCGTGATTTCGGTg                                                                                                           >  2:591523/1‑140 (MQ=255)                         gACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCGCCAGTGGATGCGTGATTTCGGTGaaa                                                                                                        <  1:591523/140‑1 (MQ=255)                                   ggtctggAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCGCCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGt                                                                                              <  1:680625/140‑1 (MQ=255)                                      ctggAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCGCCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTa                                                                                           <  2:149567/140‑1 (MQ=255)                                         gAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCGCCAGTGGATGCGTGATTTCGGTGAAAGCctg                                                                                                   >  2:1160228/1‑129 (MQ=255)                                         gAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCGCCAGTGGATGCGTGATTTCGGTGAAAGCctg                                                                                                   <  1:1160228/129‑1 (MQ=255)                                         gAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCGCCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCg                                                                                        >  1:145773/1‑140 (MQ=255)                                          aaGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCGCCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGt                                                                                       >  1:922189/1‑140 (MQ=255)                                           aGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCGCCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTc                                                                                      <  1:79344/140‑1 (MQ=255)                                              tGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCGCCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTccgc                                                                                   <  2:1270324/140‑1 (MQ=255)                                              tGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCGCCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTccgc                                                                                   <  2:70503/140‑1 (MQ=255)                                                  cacGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCGCCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGAt                                                                               <  2:145773/140‑1 (MQ=255)                                                       ttCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCGCCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGaca                                                                          >  2:375225/1‑140 (MQ=255)                                                            aCAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCGCCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGACACCCGt                                                                     <  2:776937/140‑1 (MQ=255)                                                              aaCGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCGCCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGACACCCGTTc                                                                   <  2:880979/140‑1 (MQ=255)                                                               aCGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCGCCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGACACCCGtt                                                                    >  2:1139603/1‑138 (MQ=255)                                                               aCGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCGCCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGACACCCGtt                                                                    <  1:1139603/138‑1 (MQ=255)                                                               aCGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCGCCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGACACCCGTTCg                                                                  >  2:388601/1‑140 (MQ=255)                                                               aCGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCGCCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGACACCCGTTCg                                                                  >  1:496566/1‑140 (MQ=255)                                                               aCGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCGCCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGACACCCGTTCg                                                                  >  2:949977/1‑140 (MQ=255)                                                                  ccGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCGCCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGACACCCGTTCGGTg                                                               <  1:1226297/140‑1 (MQ=255)                                                                   cGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCGCCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGACACCCGTTc                                                                   >  1:792949/1‑135 (MQ=255)                                                                   cGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCGCCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGACACCCGTTc                                                                   <  2:792949/135‑1 (MQ=255)                                                                   cGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCGCCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGACACCCGTTCGGTGa                                                              >  1:1025754/1‑140 (MQ=255)                                                                       tACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCGCCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGACACCCGTTCGGTGAaaga                                                          <  1:1340744/140‑1 (MQ=255)                                                                        acacCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCGCCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGACACCCGTTCGGTGaa                                                             >  1:1020920/1‑136 (MQ=255)                                                                        acacCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGACCGCCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGACACCCGTTCGGTGaa                                                             <  2:1020920/136‑1 (MQ=255)                                                                               cGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCGCCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGACACCCGTTCGGTGAAAGAAGTGATAg                                                  >  2:1023849/1‑140 (MQ=255)                                                                                   cACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCGCCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGACACCCGTTCGGTGAAAGAAGTGATAGGCCa                                              >  2:279182/1‑140 (MQ=255)                                                                                   cACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCGCCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGACACCCGTTCGGTGAAAGAAGTGATAGGCCa                                              >  2:1099846/1‑140 (MQ=255)                                                                                         cTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCGCCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGACACCCGTTCGGTGAAAGAAGTGATAGGCCAGAAATc                                        >  1:1309390/1‑140 (MQ=255)                                                                                         cTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCGCCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGACACCCGTTCGGTGAAAGAAGTGATAGGCCAGAAATc                                        >  1:955712/1‑140 (MQ=255)                                                                                            aTCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCGCCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGACACCCGTTCGGTGAAAGAAGTGATAGGCCAGAAATCCaa                                     >  1:585259/1‑140 (MQ=255)                                                                                              cccATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCGCCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGACACCCGTTCGGTGAAAGAAGTGATAGGCCAGAAATCCAACg                                   <  1:388601/140‑1 (MQ=255)                                                                                                  tGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCGCCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGACACCCGTTCGGTGAAAGAAGTGATAGGCCAGAAATCCAACGGCaa                               <  1:949977/140‑1 (MQ=255)                                                                                                  tGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCGCCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGACACCCGTTCGGTGAAAGAAGTGATAGGCCAGAAATCCAACGGCaa                               >  1:1325526/1‑140 (MQ=255)                                                                                                    gCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCGCCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGACACCCGTTCGGTGAAAGAAGTGATAGGCCAGAAATCCAACGGCAAcc                             <  1:517557/140‑1 (MQ=255)                                                                                                       tACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCGCCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGACACCCGTTCGGTGAAAGAAGTGATAGGCCAGAAATCCAACGGCAACCAgg                          >  1:170430/1‑140 (MQ=255)                                                                                                       tACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCGCCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGACACCCGTTCGGTGAAAGAAGTGATAGGCCAGAAATCCAACGGCAACCAgg                          >  1:1411046/1‑140 (MQ=255)                                                                                                       tACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCGCCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGACACCCGTTCGGTGAAAGAAGTGATAGGCCAGAAATCCAACGGCAACCAgg                          >  2:879974/1‑140 (MQ=255)                                                                                                       tACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCGCCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGACACCCGTTCGGTGAAAGAAGTGATAGGCCAGAAATCCAACGGCAACCAgg                          >  2:1208024/1‑140 (MQ=255)                                                                                                          gCTCTCTGGTCGTCAGCAACTGTATCAGGATCGCCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGACACCCGTTCGGTGAAAGAAGTGATAGGCCAGAAATCCAACGGCAACCAGGaaa                       <  2:1309390/140‑1 (MQ=255)                                                                                                          gCTCTCTGGTCGTCAGCAACTGTATCAGGATCGCCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGACACCCGTTCGGTGAAAGAAGTGATAGGCCAGAAATCCAACGGCAACCAGGaaa                       <  2:955712/140‑1 (MQ=255)                                                                                                              tctGGTCGTCAGCAACTGTATCAGGATCGCCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGACACCCGTTCGGTGAAAGAAGTGATAGGCCAGAAATCCAACGGCAACCAGGAAAAAgc                   >  1:1061836/1‑140 (MQ=255)                                                                                                                      tcAGCAACTGTATCAGGATCGCCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGACACCCGTTCGGTGAAAGAAGTGATAGGCCAGAAATCCAACGGCAACCAGGAAAAAGCGCTCAACt           >  2:381402/1‑140 (MQ=255)                                                                                                                               gTATCAGGATCGCCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGACACCCGTTCGGTGAAAGAAGTGATAGGCCAGAAATCCAACGGCAACCAGGAAAAAGCGCTCAACTTCCTGAcgc  >  1:681861/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                 GCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGACACCCGTTCGGTGAAAGAAGTGATAGGCCAGAAATCCAACGGCAACCAGGAAAAAGCGCTCAACTTCCTGACGC  >  NC_000913/1282872‑1283137

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑