Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,358,731	C→T	V153I (GTC→ATC)	puuP ←	putrescine:H(+) symporter PuuP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,358,731	0	C	T	100.0%	188.5 / NA	53	V153I (GTC→ATC)	puuP	putrescine:H(+) symporter PuuP
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (31/22);  total (31/22)

GACCAGACGGTGCCAACGCCTTCTCCTTTATGCAGTCCCTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGATAATCCAG  >  NC_000913/1358610‑1358866                                                                                                                          |                                                                                                                                        gACCAGACGGTGCCAACGCCTTCTCCTTTATGCAGTCCCTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGATGCTCTTCAGATTCgcggc                                                                                                                       <  1:1172942/140‑1 (MQ=255)       aCGGTGCCAACGCCTTCTCCTTTATGCAGTCCCTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGATGCTCTTCAGATTCGCGGCGGTTaa                                                                                                                 >  2:68030/1‑140 (MQ=255)           tGCCAACGCCTTCTCCTTTATGCAGTCCCTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGATGCTCTTCAGATTCGCGGCGGTTAAAATg                                                                                                             <  2:505610/140‑1 (MQ=255)               aaCGCCTTCTCCTTTATGCAGTCCCTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGATGCTCTTCAGATTCGCGGCGGTTAAAATGGcga                                                                                                         <  1:1162854/140‑1 (MQ=255)                 cGCCTTCTCCTTTATGCAGTCCCTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGATGCTCTTCAGATTCGCGGCGGTTAAAATGGcgacg                                                                                                       <  2:1392939/140‑1 (MQ=255)                 cGCCTTCTCCTTTATGCAGTCCCTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGATGCTCTTCAGATTCGCGGCGGTTAAAATGGcgacg                                                                                                       >  1:51223/1‑140 (MQ=255)                     ttCTCCTTTATGCAGTCCCTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGATGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAgg                                                                                                   >  2:1117486/1‑140 (MQ=255)                     ttCTCCTTTATGCAGTCCCTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGATGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAgg                                                                                                   >  1:1002408/1‑140 (MQ=255)                        tcCTTTATGCAGTCCCTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGATGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTa                                                                                                <  1:669542/140‑1 (MQ=255)                         ccTTTATGCAGTCCCTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGATGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTac                                                                                               <  1:369377/140‑1 (MQ=255)                                    tCCCTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGATGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACcacccacaccc                                                                                    >  2:1324329/1‑140 (MQ=255)                                    tCCCTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGATGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACcacccacaccc                                                                                    >  1:781371/1‑140 (MQ=255)                                    tCCCTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGATGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACcacccacaccc                                                                                    >  1:377354/1‑140 (MQ=255)                                    tCCCTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGATGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACcacccacaccc                                                                                    >  1:4122/1‑140 (MQ=255)                                    tCCCTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGATGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGGTACcacccacaccc                                                                                    >  2:494404/1‑140 (MQ=255)                                              aCCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGATGCTCTTCAGATTCGCGGCGGTTAAAATGGCGGCGAAGGTTACCCCCCACACCCAcggcgggcc                                                                          >  2:1357879/1‑137 (MQ=255)                                               ccAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGATGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACCCCCACGGCGGCACt                                                                         <  2:932800/140‑1 (MQ=255)                                                cAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGATGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACtt                                                                        >  1:1269344/1‑140 (MQ=255)                                                cAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGATGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACCAAGGCTAACACCCACCCCCACGGCGGCAcct                                                                        >  1:149842/1‑138 (MQ=255)                                                        tAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGATGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAag                                                                <  1:1187224/140‑1 (MQ=255)                                                         aaaCACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGATGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAaga                                                               >  1:1011106/1‑140 (MQ=255)                                                         aaaCACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGATGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAaga                                                               >  1:1293652/1‑140 (MQ=255)                                                            caccacCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGATGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAggg                                                            >  2:598998/1‑140 (MQ=255)                                                              ccaccaTGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGATGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCg                                                          <  2:4122/140‑1 (MQ=255)                                                              ccaccaTGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGATGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCg                                                          <  2:1002408/140‑1 (MQ=255)                                                                 ccaTGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGATGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGgag                                                       >  2:19122/1‑140 (MQ=255)                                                                 ccaTGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGATGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGgag                                                       >  1:5602/1‑140 (MQ=255)                                                                 ccaTGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGATGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGgag                                                       >  2:1138955/1‑140 (MQ=255)                                                                  caTGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGATGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGGTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGgaga                                                      >  1:956011/1‑140 (MQ=255)                                                                               tGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGATGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGc                                         <  1:1324329/140‑1 (MQ=255)                                                                               tGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGATGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGGTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGc                                         >  1:113248/1‑140 (MQ=255)                                                                                 cACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGATGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCca                                       >  1:1251638/1‑140 (MQ=255)                                                                                  aCCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGATGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCcaa                                      <  1:68030/140‑1 (MQ=255)                                                                                   ccAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGATGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCcaac                                     <  2:1293652/140‑1 (MQ=255)                                                                                    cAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGATGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCcaaca                                    <  1:19122/140‑1 (MQ=255)                                                                                    cAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGATGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCcaaca                                    <  1:1324917/140‑1 (MQ=255)                                                                                    cAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGATGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCcaaca                                    <  1:923490/140‑1 (MQ=255)                                                                                      gTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGATGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCcaacaaa                                  >  2:159806/1‑139 (MQ=255)                                                                                      gTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGATGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGGTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGGGAGATAGATTTTCGCcaacaaa                                  >  1:632629/1‑139 (MQ=255)                                                                                       tACAAACAGGGTATTGAAGTTAGCGACCAGGTTGATGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGGTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGa                                 >  2:204687/1‑140 (MQ=255)                                                                                                ggTATTGAAGTTAGCGACCAGGTTGATGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAatca                        >  1:1223837/1‑140 (MQ=255)                                                                                                ggTATTGAAGTTAGCGACCAGGTTGATGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAatca                        >  1:651933/1‑140 (MQ=255)                                                                                                ggTATTGAAGTTAGCGACCAGGTTGATGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAatca                        >  1:284712/1‑140 (MQ=255)                                                                                                 gTATTGAAGTTAGCGACCAGGTTGATGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAatcat                       >  1:634391/1‑140 (MQ=255)                                                                                                 gTATTGAAGTTAGCGACCAGGTTGATGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAatcat                       >  1:196208/1‑140 (MQ=255)                                                                                                    ttGAAGTTAGCGACCAGGTTGATGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCgg                    <  2:196208/140‑1 (MQ=255)                                                                                                    ttGAAGTTAGCGACCAGGTTGATGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCgg                    <  2:1269344/140‑1 (MQ=255)                                                                                                              cGACCAGGTTGATGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGGGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAgaga          >  1:945585/1‑140 (MQ=255)                                                                                                              cGACCAGGTTGATGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAgaga          <  1:305773/140‑1 (MQ=255)                                                                                                              cGACCAGGTTGATGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTCCGCCAACAAGACGTTAATCATCGGCAAAAAgaga          <  2:1251638/140‑1 (MQ=255)                                                                                                                 ccAGGTTGATGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAgaga          <  1:785685/137‑1 (MQ=255)                                                                                                                 ccAGGTTGATGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAgaga          >  2:785685/1‑137 (MQ=255)                                                                                                                      ttGATGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGATAATCcag  <  2:651933/140‑1 (MQ=255)                                                                                                                          |                                                                                                                                        GACCAGACGGTGCCAACGCCTTCTCCTTTATGCAGTCCCTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGATAATCCAG  >  NC_000913/1358610‑1358866

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑