Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,364,806	A→G	D192G (GAC→GGC)	puuB →	gamma‑glutamylputrescine oxidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,364,806	0	A	G	100.0%	261.2 / NA	77	D192G (GAC→GGC)	puuB	gamma‑glutamylputrescine oxidase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (34/43);  total (34/43)

ACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGCCGTGAAGTAGCCAGCGATCGCTATACCGGTGCGCTGCTGGATCACAGCGGTGGGCATATTCATCCGCTAAACCTTGCCATTGGCGAAGCGGACGCCATCCGCCTCAACGGCGGGCGCGTGTATGAACTTTCTGCCGTGACGCAGATCCAGCACACCACGCCAGCCGTTGTGCGAACTGCCAAAGGTCAGGTGACGGCGAAGTATGTGATTGTCGCCGGGAATG  >  NC_000913/1364671‑1364937                                                                                                                                        |                                                                                                                                    aCGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGCCGTGAGGTAGCCAGCGATCGCTATACCGGTGCGCTGCTGGATCACAGCGGTGGGCATATTCATCCGCTAAACCTTGCCATTGGCGAAGCGGGCGcc                                                                                                                                 >  2:184952/1‑140 (MQ=255) aCGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGCCGTGAAGTAGCCAGCGATCGCTATACCGGTGCGCTGCTGGATCACAGCGGTGGGCATATTCATCCGCTAAACCTTGCCATTGGCGAAGCGGGCGcc                                                                                                                                 <  2:708093/140‑1 (MQ=255) aCGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGCCGTGAAGTAGCCAGCGATCGCTATACCGGTGCGCTGCTGGATCACAGCGGTGGGCATATTCATCCGCTAAACCTTGCCATTGGCGAAGCGGGCGcc                                                                                                                                 >  2:692593/1‑140 (MQ=255)  cGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGCCGTGAAGTAGCCAGCAATCGCTATACCGGTGCGCTGCTGGATCACAGCGGTGGGCATATTAATCCGCTAAACCTTGCCATTGGCGAAGCGGGCGCCa                                                                                                                                <  1:901903/140‑1 (MQ=255)   ggCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGCCGTGAAGTAGCCAGCGATCGCTATACCGGTGCGCTGCTGGATCACAGCGGTGGGCATATTCATCCGCTAAACCTTGCCATTGGCGAAGCGGGCGCCAt                                                                                                                               >  2:574456/1‑140 (MQ=255)        tAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGCCGTGAAGTAGCCAGCGATCGCTATACCGGTGCGCTGCTGGATCACAGCGGTGGGCATATTCATCCGCTAAACCTTGCCATTGGCGAAGCGGGCGCCATCCGcc                                                                                                                          >  2:649087/1‑140 (MQ=255)        tAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGCCGTGAAGTAGCCAGCGATCGCTATACCGGTGCGCTGCTGGATCACAGCGGTGGGCATATTCATCCGCTAAACCTTGCCATTGGCGAAGCGGGCGCCATCCGcc                                                                                                                          >  2:568293/1‑140 (MQ=255)               atGGAATTGCTGGACGCCAACGCCATTCGCCGTGAAGTAGCCAGCGATCGCTATACCGGTGCGCTGCTGGATCACAGCGGTGGGCATATTCATCCGCTAAACCTTGCCATTGGCGAAGCGGGCGCCATCCGCCTCAAcgg                                                                                                                   >  2:1236112/2‑140 (MQ=255)                  gAATTGCTGGACGCCAACGCCATTCGCCGTGAAGTAGCCAGCGATCGCTATACCGGTGCGCTGCTGGATCACAGCGGTGGGCATATTCATCCGCTAAACCTTGCCATTGGCGAAGCGGGCGCCATCCGCCTCAAcggcgg                                                                                                                >  2:194561/1‑140 (MQ=255)                     ttGCTGGACGCCAACGCCATTCGCCGTGAAGTAGCCAGCGATCGCTATACCGGTGCGCTGCTGGATCACAGCGGTGGGCATATTCATCCGCTAAACCTTGCCATTGGCGAAGCGGGCGCCATCCGCCTCAACggcgggcg                                                                                                             <  2:1037722/140‑1 (MQ=255)                            acgccaacgccaTTCGCCGTGAAGTAGCCAGCGATCGCTATACCGGTGCGCTGCTGGATCACAGCGGTGGGCATATTCATCCGCTAAACCTTGCCATTGGCGAAGCGGGCGCCATCCGCCTCAACGGCGGGCGCGTGTAt                                                                                                      <  2:1027079/140‑1 (MQ=255)                             cgccaacgccaTTCGCCGTGAAGTAGCCAGCGATCGCTATACCGGTGCGCTGCTGGATCACAGCGGTGGGCATATTCATCCGCTAAACCTTGCCATTGGCGAAGCGGGCGCCATCCGCCTCAACGGCGGGCGCGTGTATg                                                                                                     <  1:1392476/140‑1 (MQ=255)                             cgccaacgccaTTCGCCGTGAAGTAGCCAGCGATCGCTATACCGGTGCGCTGCTGGATCACAGCGGTGGGCATATTCATCCGCTAAACCTTGCCATTGGCGAAGCGGGCGCCATCCGCCTCAACGGCGGGCGCGTGTATg                                                                                                     >  2:1334769/1‑140 (MQ=255)                                 aacgccaTTCGCCGTGAAGTAGCCAGCGATCGCTATACCGGTGCGCTGCTGGATCACAGCGGTGGGCATATTCATCCGCTAAACCTTGCCATTGGCGAAGCGGGCGCCATCCGCCTCAACGGCGGGCGCGTGTATGAACt                                                                                                 >  1:606269/1‑140 (MQ=255)                                 aacgccaTTCGCCGTGAAGTAGCCAGCGATCGCTATACCGGTGCGCTGCTGGATCACAGCGGTGGGCATATTCATCCGCTAAACCTTGCCATTGGCGAAGCGGGCGCCATCCGCCTCAACGGCGGGCGCGTGTATGAACt                                                                                                 <  1:194561/140‑1 (MQ=255)                                         tCGCCGTGAAGTAGCCAGCGATCGCTATACCGGTGCGCTGCTGGATCACAGCGGTGGGCATATTCATCCGCTAAACCTTGCCATTGGCGAAGCGGGCGCCATCCGCCTCAACGGCGGGCGCGTGTATGAACTTTCTGCCg                                                                                         <  2:596702/140‑1 (MQ=255)                                         tCGCCGTGAAGTAGCCAGCGATCGCTATACCGGTGCGCTGCTGGATCACAGCGGTGGGCATATTCATCCGCTAAACCTTGCCATTGGCGAAGCGGGCGCCATCCGCCTCAACGGCGGGCGCGTGTATGAACTTTCTGCCg                                                                                         <  2:373103/140‑1 (MQ=255)                                          cGCCGTGAAGTAGCCAGCGATCGCTATACCGGTGCGCTGCTGGATCACAGCGGTGGGCATATTCATCCGCTAAACCTTGCCATTGGCGAAGCGGGCGCCATCCGCCTCAACGGCGGGCGCGTGTATGAACTTTCTGCCGt                                                                                        <  1:800853/140‑1 (MQ=255)                                          cGCCGTGAAGTAGCCAGCGATCGCTATACCGGTGCGCTGCTGGATCACAGCGGTGGGCATATTCATCCGCTAAACCTTGCCATTGGCGAAGCGGGCGCCATCCGCCTCAACGGCGGGCGCGTGTATGAACTTTCTGCCGt                                                                                        <  1:873420/140‑1 (MQ=255)                                              gTGAAGTAGCCAGCGATCGCTATACCGGTGCGCTGCTGGATCACAGCGGTGGGCATATTCATCCGCTAAACCTTGCCATTGGCGAAGCGGGCGCCATCCGCCTCAACGGCGGGCGCGTGTATGAACTTTCTGCCGTGACg                                                                                    <  1:934576/140‑1 (MQ=255)                                               tGAAGTAGCCAGCGATCGCTATACCGGTGCGCTGCTGGATCACAGCGGTGGGCATATTCATCCGCTAAACCTTGCCATTGGCGAAGCGGGCGCCATCCGCCTCAACGGCGGGCGCGTGTATGAACTTTCTGCCGTGACGc                                                                                   <  1:554454/140‑1 (MQ=255)                                               tGAAGTAGCCAGCGATCGCTATACCGGTGCGCTGCTGGATCACAGCGGTGGGCATATTCATCCGCTAAACCTTGCCATTGGCGAAGCGGGCGCCATCCGCCTCAACGGCGGGCGCGTGTATGAACTTTCTGCCGTGACGc                                                                                   <  1:1236112/140‑1 (MQ=255)                                                gAAGTAGCCAGCGATCGCTATACCGGTGCGCTGCTGGTTCACAGCGGTGGGCATATTCATCCGCTAAACCTTGCCATTGGCGAAGCGGGCGCCATCCGCCTCAACGGCGGGCGCGTGTATGAACTTTCTGCCGTGACGCa                                                                                  <  2:1300656/140‑1 (MQ=255)                                                 aaGTAGCCAGCGATTGCTATACCGGTGCGCTGCTGGATCACAGCGGTGGGCATATTCATCCGCTAAACCTTGCCATTGGCGAAGCGGGCGCCATCCGCCTCAACGGCGGGCGCGTGTATGAACTTTCTGCCGTGACGCAg                                                                                 <  2:869635/140‑1 (MQ=255)                                                 aaGTAGCCAGCGATCGCTATACCGGTGCGCTGCTGGATCACAGCGGTGGGCATATTCATCCGCTAAACCTTGCCATTGGCGAAGCGGGCGCCATCCGCCTCAACGGCGGGCGCGTGTATGAACTTTCTGCCGTGACGCAg                                                                                 <  2:401311/140‑1 (MQ=255)                                                  aGTAGCCAGCGATCGCTATACCGGTGCGCTGCTGGATCACAGCGGTGGGCATATTCATCCGCTAAACCTTGCCATTGGCGAAGCGGGCGCCATCCGCCTCAACGGCGGGCGCGTGTATGAACTTTCTGCCGTGAc                                                                                     <  1:1207673/135‑1 (MQ=255)                                                  aGTAGCCAGCGATCGCTATACCGGTGCGCTGCTGGATCACAGCGGTGGGCATATTCATCCGCTAAACCTTGCCATTGGCGAAGCGGGCGCCATCCGCCTCAACGGCGGGCGCGTGTATGAACTTTCTGCCGTGAc                                                                                     >  2:1207673/1‑135 (MQ=255)                                                  aGTAGCCAGCGATCGCTATACCGGTGCGCTGCTGGATCACAGCGGTGGGCATATTCATCCGCTAAACCTTGCCATTGGCGAAGCGGGCGCCATCCGCCTCAACGGCGGGCGCGTGTATGAACTTTCTGCCGTGACGCAGa                                                                                <  1:765346/140‑1 (MQ=255)                                                    tAGCCAGCGATCGCTATACCGGTGCGCTGCTGGATCACAGCGGTGGGCATATTCATCCGCTAAACCTTGCCATTGGCGAAGCGGGCGCCATCCGCCTCAACGGCGGGCGCGTGTATGAACTTTCTGCCGTGACGCAGATc                                                                              <  2:688652/140‑1 (MQ=255)                                                         aGCGATCGCTATACCGGTGCGCTGCTGGATCACAGCGGTGGGCATATTCATCCGCTAAACCTTGCCATTGGCGAAGCGGGCGCCATCCGCCTCAACGGCGGGCGCGGGTATGAACTTTCTGCCGTGACGCAGATCCCGcc                                                                         >  2:954595/1‑139 (MQ=255)                                                               cGCTATACCGGTGCGCTGCTGGATCACAGCGGTGGGCATATTCATCCGCTAAACCTTGCCATTGGCGAAGCGGGCGCCATCCGCCTCAACGGCGGGCGCGTGTATGAACTTTCTGCCGTGACGCAGATCCAGCAcaccac                                                                   <  2:547005/140‑1 (MQ=255)                                                                 cTATACCGGTGCGCTGCTGGATCACAGCGGTGGGCATATTCATCCGCTAAACCTTGCCATTGGCGAAGCGGGCGCCATCCGCCTCAACGGCGGGCGCGTGTATGAACTTTCTGCCGTGACGCAGATCCAGCACACCACGc                                                                 <  1:435775/140‑1 (MQ=255)                                                                    taCCGGTGCGCTGCTGGATCACAGCGGTGGGCATATTCATCCGCTAAACCTTGCCATTGGCGAAGCGGGCGCCATCCGCCTCAACGGCGGGCGCGTGTATGAACTTTCTGCCGTGACGCAGATCCAGCACACCACGCCAg                                                              <  2:494416/140‑1 (MQ=255)                                                                       cGGTGCGCTGCTGGATCACAGCGGTGGGCATATTCATCCGCTAAACCTTGCCATTGGCGAAGCGGGCGCCATCCGCCTCAACGGCGGGCGCGTGTATGAACTTTCTGCCGTGACGCAGATCCAGCACACCACGCCAGCCg                                                           <  1:1149953/140‑1 (MQ=255)                                                                         gTGCGCTGCTGGATCACAGCGGTGGGCATATTCATCCGCTAAACCTTGCCATTGGCGAAGCGGGCGCCATCCGCCTCAACGGCGGGCGCGTGTATGAACTTTCTGCCGTGACGCAGATCCCGCACACCACGCCAGCCggt                                                         >  2:967435/1‑138 (MQ=255)                                                                         gTGCGCTGCTGGATCACAGCGGTGGGCATATTCATCCGCTAAACCTTGCCATTGGCGAAGCGGGCGCCATCCGCCTCAACGGCGGGCGCGTGTATGAACTTTCTGCCGTGACGCAGATCCAGCACACCACGCCAGCCGtt                                                         >  1:728457/1‑140 (MQ=255)                                                                         gTGCGCTGCTGGATCACAGCGGTGGGCATATTCATCCGCTAAACCTTGCCATTGGCGAAGCGGGCGCCATCCGCCTCAACGGCGGGCGCGGGTATGAAATTTCTGCCGTGACGCAGATCCAGCACACCACGCCAGCCgct                                                         >  1:432590/1‑138 (MQ=255)                                                                         gTGCGCTGCTGGATCACAGCGGTGGGCATATTCATCCGCTAAACCTTGCCATTGGCGAAGCGGGCGCCATCCGCCTCAACGGCGGGCGCGGGTATGAAATTTCTGCCGTGACGCAGAACCAGCACACCACGCCCGCCGtt                                                         >  2:943772/1‑140 (MQ=255)                                                                         gTGCGCTGCTGGATCACAGCGGTGGGCATATTCATCCGCTAAACCTTGCCATTGGCGAAGCGGGCGCCATCCGCCTCAACGGCGGGCGCGGGGATGAACTTTCTGCCGTGACGCAGATCCAGCACACCACGCCAGCCGtt                                                         >  2:1199988/1‑140 (MQ=255)                                                                         gTGCGCTGCTGGATCACAGCGGTGGGCATATTCATCCGCTAAACCTTGCCATTGGCGAAGCGGGCGCCATCCGCCTCAACGGCGGGCGCGGGGATGAAATTTCTGCCGTGACGCAGATCCAGCACACCATGCCAGCCGtt                                                         >  2:343288/1‑140 (MQ=255)                                                                          tGCGCTGCTGGATCACAGCGGTGGGCATATTCATCCGCTAAACCTTGCCATTGGCGAAGCGGGCGCCATCCGCCTCAACGGCGGGCGGGGGTATGATCATTTGCCCGGGCCGCCGCTCCCGCACACCACGCCCGGCGTtg                                                        >  2:1169182/1‑140 (MQ=255)                                                                            cgcTGCTGGATCACAGCGGTGGGCATATTCATCCGCTAAACCTTGCCATTGGCGAAGCGGGCGCCATCCGCCTCAACGGCGGGCGGGTGTTTGAACTTTCTGCCCTGACGCAGATCCACCACACCACCCCAGCCggtttg                                                      >  2:435775/1‑135 (MQ=255)                                                                            cgcTGCTGGATCACAGCGGTGGGCATATTCATCCGCTAAACCTTGCCATTGGCGAAGCGGGCGCCATCCGCCTCAACGGCGGGCGCGTGTATGAACTTTCTGCCGTGACGCAGATCCAGCACACCACGCCAGCCGTtgtg                                                      >  1:190133/1‑140 (MQ=255)                                                                            cgcTGCTGGATCACAGCGGTGGGCATATTCATCCGCTAAACCTTGCCATTGGCGAAGCGGGCGCCATCCGCCTCAACGGCGGGCGCGTGTATGAACTTTCTGCCGTGACGCAGATCCAGCACACCACGCCAGCCGTtgtg                                                      >  1:1260853/1‑140 (MQ=255)                                                                            cgcTGCTGGATCACAGCGGTGGGCATATTCATCCGCTAAACCTTGCCATTGGCGAAGCGGGCGCCATCCGCCTCAACGGCGGGCGCGTGTATGAACTTTCTGCCGTGACGCAGATCCAGCACACCACGCCAGCCGTtgtg                                                      >  1:178898/1‑140 (MQ=255)                                                                            cgcTGCTGGATCACAGCGGTGGGCATATTCATCCGCTAAACCTTGCCATTGGCGAAGCGGGCGCCATCCGCCTCAACGGCGGGCGCGTGTATGAACTTTCTGCCGTGACGCAGATCCAGCACACCACGCCAGCCGTtgtg                                                      >  1:532428/1‑140 (MQ=255)                                                                            cgcTGCTGGATCACAGCGGTGGGCATATTCATCCGCTAAACCTTGCCATTGGCGAAGCGGGCGCCATCCGCCTCAACGGCGGGCGCGTGTATGAACTTTCTGCCGTGACGCAGATCCAGCACACCACGCCAGCCGTtgtg                                                      >  1:943885/1‑140 (MQ=255)                                                                            cgcTGCTGGATCACAGCGGTGGGCATATTCATCCGCTAAACCTTGCCATTGGCGAAGCGGGCGCCATCCGCCTCAACGGCGGGCGCGGGTATTAAATTTCTGCCGGGACGCCGATCCAGCACACCACGCCCGCCCTtgtg                                                      >  2:495853/1‑140 (MQ=255)                                                                              ctgctgGATCACAGCGGTGGGCATATTCATCCGCTAAACCTTGCCATTGGCGAAGCGGGCGCCATCCGCCTCAACGGCGGGCGCGTGTATGAACTTTCTGCCGTGACGCAGATCCAGCACACCACGCCAg                                                              <  2:126952/130‑1 (MQ=255)                                                                              ctgctgGATCACAGCGGTGGGCATATTCATCCGCTAAACCTTGCCATTGGCGAAGCGGGCGCCATCCGCCTCAACGGCGGGCGCGTGTATGAACTTTCTGCCGTGACGCAGATCCAGCACACCACGCCAg                                                              >  1:126952/1‑130 (MQ=255)                                                                              ctgctgGATCACAGCGGTGGGCATATTCATCCGCTAAACCTTGCCATTGGCGAAGCGGGCGCCATCCGCCTCAACGGCGGGCGCGCGTATGAACTTTTTGCCGTGACGCAGATCCAGCACACCACGCCCGCCGGTTTGCg                                                    >  2:448829/1‑140 (MQ=255)                                                                                gctgGATCACAGCGGTGGGCATATTCATCCGCTAAACCTTGCCATTGGCGAAGCGGGCGCCATCCGCCTCAACGGCGGGCGCGTGTATGAACTTTCTGCCGTGACGCAGATCCAGCACACCACGCCAGCCGTTGTGCGaa                                                  <  2:35299/140‑1 (MQ=255)                                                                                      tCACAGCGGTGGGCATATTCATCCGCTAAACCTTGCCATTGGCGAAGCGGGCGCCATCCGCCTCAACGGCGGGCGCGTGTATGAACTTTCTGCCGTGACGCAGATCCAGCACACCACGCCAGCCGTTGTGCGAACTGCCa                                            <  2:606269/140‑1 (MQ=255)                                                                                      tCACAGCGGTGGGCATATTCATCCGCTAAACCTTGCCATTGGCGAAGCGGGCGCCATCCGCCTCAACGGCGGGCGCGTATATGAACTTTCTGCCGTGACGCAGATCCAGCACACCACGCCAGCCGTTGTGCGAACTGCCa                                            <  2:1260853/140‑1 (MQ=255)                                                                                           gCGGTGGGCATATTCATCCGCTAAACCTTGCCATTGGCGAAGCGGGCGCCATCCGCCTCAACGGCGGGCGCGTGTATGAACTTTCTGCCGTGACGCAGATCCAGCACACCACGCCAGCCGTTGTGCGAACTGCCAAAGGt                                       <  1:1173014/140‑1 (MQ=255)                                                                                            cGGTGGGCATATTCATCCGCTAAACCTTGCCATTGGCGAAACGGGCGCCATCCGCCTCAACGGCGGGCGCGTGGATGAACTTTCTGCCGTGACGCAGATCCAGCACACCACGCCAGCCGTTGTGCGAACTGCCAAAGGTc                                      >  2:1310355/1‑140 (MQ=255)                                                                                                 ggCATATTCATCCGCTAAACCTTGCCATTGGCGAAGCGGGCGCCATCCGCCTCAACGGCGGGCGCGGGTATGAACTTTCTGCCGTGACGCAGATCCAGCACACCCCCCCCGCCGGTGTGGGAACTGCCCAAAGTCAGGTg                                 >  2:540423/1‑140 (MQ=255)                                                                                                   cATATTCATCCGCTAAACCTTGCCATTGGCGAAGCGGGCGCCATCCGCCTCAACGGCGGGCGCGTGTATGAACTTTCTGCCGTGACGCAGATCCAGCACACCACGCCAGCCGTTGTGCGAACTGCCAAAGGTCAGGTGAc                               <  1:692593/140‑1 (MQ=255)                                                                                                     tatTCATCCGCTAAACCTTGCCATTGGCGAAGCGGGCGCCATCCGCCTCAACGGCGGGCGCGTGTATGAACTTTCTGCCGTGACGCAGATCCAGCACACCACGCCAGCCGTTGTGCGAACTGCCAAAGGTCAGGTGACgg                             <  1:574456/140‑1 (MQ=255)                                                                                                       ttCATCCGCTAAACCTTGCCATTGGCGAAGCGGGCGCCATCCGCCTCAACGGCGGGCGCGTGTATGAACTTTCTGCCGTGACGCAGATCCAGCACACCACGCCAGCCGTTGTGCGAACTGCCAAAGGTCAGGTGACGGCg                           <  2:1252718/140‑1 (MQ=255)                                                                                                       ttCATCCGCTAAACCTTGCCATTGGCGAAGCGGGCGCCATCCGCCTCAACGGCGGGCGCGTGTATGAACTTTCTGCCGTGACGCAGATCCAGCACACCACGCCAGCCGTTGTGCGAACTGCCAAAGGTCAGGTGACGGCg                           <  1:1334769/140‑1 (MQ=255)                                                                                                       ttCATCCGCTAAACCTTGCCATTGGCGAAGCGGGCGCCATCCGCCTCAACGGCGGGCGCGTGTATGAACTTTCTGCCGTGACGCAGATCCAGCACACCACGCCAGCCGTTGTGCGAACTGCCAAAGGTCAGGTGACGGCg                           <  1:135041/140‑1 (MQ=255)                                                                                                       ttCATCCGCTAAACCTTGCCATTGGCGAAGCGGGCGCCATCCGCCTCAACGGCGGGCGCGTGTATGAACTTTCTGCCGTGACGCAGATCCAGCACACCACGCCAGCCGTTGTGCGAACTGCCAAAGGTCAGGTGACGGCg                           <  2:897222/140‑1 (MQ=255)                                                                                                       ttCATCCGCTAAACCTTGCCATTGGCGAAGCGGGCGCCATCCGCCTCAACGGCGGGCGCGTGTATGAACTTTCTGCCGTGACGCAGATCCAGCACACCACGCCAGCCGTTGTGCGAACTGCCAAAGGTCAGGTGACGGCg                           <  1:568293/140‑1 (MQ=255)                                                                                                         cATCCGCTAAACCTTGCCATTGGCGAAGCGGGCGCCATCCGCCTCAACGGCGGGCGCGTGTATGAACTTTCTGCCGTGACGCAGATCCAGCACACCACGCCAGCCGTTGTGCGAACTGCCAAAGGTCAGGTGACGGCGaa                         <  2:178898/140‑1 (MQ=255)                                                                                                           tCCGCTAAACCTTGCCATTGGCGAAGCGGGCGCCATCCGCCTCAACGGCGGGCGCGTGTATGAAATTTCTGCCGTGGCGCCGATTCAGCCCACCACGCCAGCCGGTGTGCGGACTGCCAAAGGGCAGGTGACGGCGGAgg                       >  2:1300820/1‑139 (MQ=255)                                                                                                           tCCGCTAAACCTTGCCATTGGCGAAGCGGGCGCCATCCGCCTCAACGGCGGGCGCGGGTATGAACTTTCTGCCGTGACGCAGATCCAGCACACCCCGCCAGCCGTTGTGCGGACTGCCCAAGGGCAGGGGACGGCGAAgg                       >  2:164830/1‑139 (MQ=255)                                                                                                            ccGCTAAACCTTGCCATTGGCGAAGCGGGCGCCATCCGCCTCAACGGCGGGCGCGGGTATGAACTTTCTGCCGGGACGCCGATCCAGCACACCACGCCCGCCGTTGTGCGAACTGCCCAAGGGCAGGTGGCGGCGAAgga                      >  2:173015/1‑138 (MQ=255)                                                                                                               cTAAACCTTGCCATTGGCGAAGCGGGCGCCATCCGCCTCAACGGCGGGCGCGTGTATGAACTTTCTGCCGTGACGCAGATCCAGCACACCCCCCCAGCCGTTGTGCGAACTGCCCAAGGGCAGGGGACGGCGAAGTAtgt                   >  2:879299/1‑140 (MQ=255)                                                                                                                 aaaCCTTGCCATTGGCGAAGCGGGCGCCATCCGCCTCAACGGCGGGCGCGTGTATGAACTTTCTGCCGTGACGCAGATCCAGCACACCACGCCAGCCGTTGTGCGAACTGCCAAAGGTCAGGTGACGGCGAGGTATGTGa                 <  2:224346/140‑1 (MQ=255)                                                                                                                           aTTGGCGAAGCGGGCGCCATCCGCCTCAACGGCGGGCGCGTGTATGAACTTTCTGCCGTGACGCAGATCCAGCACACCACGCCAGCCGTTGTGCGAACTGCCAAAGGTCAGGTGACGGCGAAGTATGTGATTGTCGCCgg       <  1:649087/140‑1 (MQ=255)                                                                                                                           aTTGGCGAAGCGGGCGCCATCCGCCTCAACGGCGGGCGCGTGTATGAACTTTCTGCCGTGACGCAGATCCAGCACACCACGCCAGCCGTTGTGCGAACTGCCAAAGGTCAGGTGACGGCGAAGTATGTGATTGTCGCCgg       <  1:717395/140‑1 (MQ=255)                                                                                                                            ttGGCGAAGCGGGCGCCATCCGCCTCAACGGCGGGCGCGTGTATGAACTTTCTGCCGTGACGCAGATCCAGCACACCACGCCAGCCGTTGTGCGAACTGCCAAAGGTCAGGTGACGGCGAAGTATGTGATTGTCGCCggg      <  1:285185/140‑1 (MQ=255)                                                                                                                             tGGCGAAGCGGGCGCCATCCGCCTCAACGGCGGGCGCGTGTATGAACTTTCTGCCGTGACGCAGATCCAGCACACCACGCCAGCCGTTGTGCGAACTGCCAAAGGTCAGGTGACGGCGAAGTATGTGATTGTCGCCGGGa     <  2:738525/140‑1 (MQ=255)                                                                                                                             tGGCGAAGCGGCCGCCATCCGCCTCAACGGCGGGCGCGTGTATGAACTTTCTGCCGTGACGCAGATCCAGCACCCCACGCCAGCCGTTGTGCGAACTGCCAAAGGTCAGGTGACGGCGAAGTATGTGATTGTCGCCGGGa     <  2:878215/140‑1 (MQ=255)                                                                                                                              ggCGAAGCGGGCGCCATCCGCCTCAACGGCGGGCGCGTGTATGAACTTTCTGCCGTGACGCAGATCCAGCACACCACGCCAGCCGT‑‑TGCGAACTGCCAAAGGTCAGGTGACGGCGAAGTATGTGATTGTCGCCGGGAATg  <  1:313708/140‑1 (MQ=255)                                                                                                                               gCGAAGCGGGCGCCATCCGCCTCAACGGCGGGCGCGTGTATGAACTTTCTGCCGTGACGCAGATCCAGCACACCACGCCAGCCGTTGTGCGAACTGCCAAAGGTCAGGTGACGGCGAAGTAtgt                   <  1:144291/124‑1 (MQ=255)                                                                                                                               gCGAAGCGGGCGCCATCCGCCTCAACGGCGGGCGCGTGTATGAACTTTCTGCCGTGACGCAGATCCAGCACACCACGCCAGCCGTTGTGCGAACTGCCAAAGGTCAGGTGACGGCGAAGTAtgt                   >  2:144291/1‑124 (MQ=255)                                                                                                                                        |                                                                                                                                    ACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGCCGTGAAGTAGCCAGCGATCGCTATACCGGTGCGCTGCTGGATCACAGCGGTGGGCATATTCATCCGCTAAACCTTGCCATTGGCGAAGCGGACGCCATCCGCCTCAACGGCGGGCGCGTGTATGAACTTTCTGCCGTGACGCAGATCCAGCACACCACGCCAGCCGTTGTGCGAACTGCCAAAGGTCAGGTGACGGCGAAGTATGTGATTGTCGCCGGGAATG  >  NC_000913/1364671‑1364937

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑