Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,384,956	C→T	D280D (GAC→GAT)	ycjX →	DUF463 domain‑containing protein YcjX

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,384,956	0	C	T	100.0%	199.8 / NA	57	D280D (GAC→GAT)	ycjX	DUF463 domain‑containing protein YcjX
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (33/24);  total (33/24)

GGGGCGAGTCCAAACTGGCGCAGGCCGATAAGCATACCAATGCCGGAATGCTGCGCGAGCGCTTTAATTATTACTGCGAGAAGGTGGTGAAGGGGTTCTATAAGAATCATTTTCTGCGCTTTGACCGCCAGATTGTGCTGGTGGATTGCCTGCAACCTCTCAACAGTGGGCCACAGGCATTTAATGATATGCGTCTGGCACTGACGCAGCTGATGCAAAGTTTTCACTACGGGCAGCGTACCCT  >  NC_000913/1384832‑1385075                                                                                                                             |                                                                                                                        ggggCGAGTCCAAACTGGCGCAGGCCGATAAGCATACCAATGCCGGAATGCTGCGCGAGCGCTTTAATTATTACTGCGAGAAGGTGGTGAAGGGGTTCTATAAGAATCATTTTCTGCGCTTTGATCGCCAGATTGTGCtg                                                                                                          >  1:389393/1‑140 (MQ=255)  gggCGAGTCCAAACTGGCGCAGGCCGATAAGCATACCAATGCCGGAATGCTGCGCGAGCGCTTTAATTATTACTGCGAGGAGGTGGTGAAGGGGTTCTATAAGAATCATTTTCTGCGCTTTGATCGCCAGATTGTGCtgg                                                                                                         >  2:1262601/1‑140 (MQ=255)  gggCGAGTCCAAACTGGCGCAGGCCGATAAGCATACCAATGCCGGAATGCTGCGCGAGCGCTTTAATTATTACTGCGAGAAGGTGGTGAAGGGGTTCTATAAGAATCATTTTCTGCGCTTTGATCGCCAGATTGTGCtgg                                                                                                         >  1:391650/1‑140 (MQ=255)  gggCGAGTCCAAACTGGCGCAGGCCGATAAGCATACCAATGCCGGAATGCTGCGCGAGCGCTTTAATTATTACTGCGAGAAGGTGGTGAAGGGGTTCTATAAGAATCATTTTCTGCGCTTTGATCGCCAGATTGTGCtgg                                                                                                         >  1:457843/1‑140 (MQ=255)   ggCGAGTCCAAACTGGCGCAGGCCGATAAGCATACCAATGCCGGAATGCTGCGCGAGCGCTTTAATTATTACTGCGAGAAGGTGGTGAAGGGGTTCTATAAGAATCATTTTCTGCGCTTTGATCGCCAGATTGTGCtggt                                                                                                        >  2:543570/1‑140 (MQ=255)   ggCGAGTCCAAACTGGCGCAGGCCGATAAGCATACCAATGCCGGAATGCTGCGCGAGCGCTTTAATTATTACTGCGAGAAGGTGGTGAAGGGGTTCTATAAGAATCATTTTCTGCGCTTTGATCGCCAGATTGTGCtggt                                                                                                        >  2:790453/1‑140 (MQ=255)      gAGTCCAAACTGGCGCAGGCCGATAAGCATACCAATGCCGGAATGCTGCGCGAGCGCTTTAATTATTACTGCGAGAAGGTGGTGAAGGGGTTCTATAAGAATCATTTTCTGCGCTTTGATCGCCAGATTGTGCTGGTGGa                                                                                                     <  2:389393/140‑1 (MQ=255)      gAGTCCAAACTGGCGCAGGCCGATAAGCATACCAATGCCGGAATGCTGCGCGAGCGCTTTAATTATTACTGCGAGAAGGTGGTGAAGGGGTTCTATAAGAATCATTTTCTGCGCTTTGATCGCCAGATTGTGCTGGTGGa                                                                                                     <  2:304822/140‑1 (MQ=255)         tCCAAACTGGCGCAGGCCGATAAGCATACCAATGCCGGAATGCTGCGCGAGCGCTTTAATTATTACTGCGAGAAGGTGGTGAAGGGGTTCTATAAGAATCATTTTCTGCGCTTTGATCGCCAGATTGTGCTGGTGGATTg                                                                                                  <  1:453791/140‑1 (MQ=255)           cAAACTGGCGCAGGCCGATAAGCATACCAATACCGGAATGCTGCGCGAGCGCTTTAATTATTACTGCGAGAAGGTGGTGAAGGGGTTCTATAAGAATCATTTTCTGCGCTTTGATCGCCAGATTGTGCTGGTGGATTGcc                                                                                                >  1:1305133/1‑140 (MQ=255)              aCTGGCGCAGGCCGATAAGCATACCAATGCCGGAATGCTGCGCGAGCGCTTTAATTATTACTGCGAGAAGGTGGTGAAGGGGTTCTATAAGAATCATTTTCTGCGCTTTGATCGCCAGATTGTGCTGGTGGATTGCCTGc                                                                                             <  1:291815/140‑1 (MQ=255)               cTGGCGCAGGCCGATAAGCATACCAATGCCGGAATGCTGCGCGAGCGCTTTAATTATTACTGCGAGAAGGTGGTGAAGGGGTTCTATAAGAATCATTTTCTGCGCTTTGATCGCCAGATTGTGCTGGTGGATTGCCTGCa                                                                                            <  1:1069806/140‑1 (MQ=255)                tGGCGCAGGCCGATAAGCATACCAATGCCGGAATGCTGCGCGAGCGCTTTAATTATTACTGCGAGAAGGTGGTGAAGGGGTTCTATAAGAATCATTTTCTGCGCTTTGATCGCCAGATTGTGCTGGTGGATTGCCTGCaa                                                                                           <  1:764810/140‑1 (MQ=255)                tGGCGCAGGCCGATAAGCATACCAATGCCGGAATGCTGCGCGAGCGCTTTAATTATTACTGCGAGAAGGTGGTGAAGGGGTTCTATAAGAATCATTTTCTGCGCTTTGATCGCCAGATTGTGCTGGTGGATTGCCTGCaa                                                                                           <  1:45729/140‑1 (MQ=255)                 ggCGCAGGCCGATAAGCATACCAATGCCGGAATGCTGCGCGAGCGCTTTAATTATTACTGCGAGAAGGTGGTGAAGGGGTTCTATAAGAATCATTTTCTGCGCTTTGATCGCCAGATTGTGCTGGTGGATTGCCTGCAAc                                                                                          <  2:457843/140‑1 (MQ=255)                  gcgcAGGCCGATAAGCATACCAATGCCGGAATGCTGCGCGAGCGCTTTAATTATTACTGCGAGAAGGTGGTGAAGGGGTTCTATAAGAATCATTTTCTGCGCTTTGATCGCCAGATTGTGCTGGTGGATTGCCTGCAAcc                                                                                         >  2:1162537/1‑140 (MQ=255)                  gcgcAGGCCGATAAGCATACCAATGCCGGAATGCTGCGCGAGCGCTTTAATTATTACTGCGAGAAGGTGGTGAAGGGGTTCTATAAGAATCATTTTCTGCGCTTTGATCGCCAGATTGTGCTGGTGGATTGCCTGCAAcc                                                                                         >  2:716908/1‑140 (MQ=255)                  gcgcAGGCCGATAAGCATACCAATGCCGGAATGCTGCGCGAGCGCTTTAATTATTACTGCGAGAAGGTGGTGAAGGGGTTCTATAAGAATCATTTTCTGCGCTTTGATCGCCAGATTGTGCTGGTGGATTGCCTGCAAcc                                                                                         >  1:1101045/1‑140 (MQ=255)                   tgcAGGCCGATAAGCATACCAATGCCGGAATGCTGCGCGAGCGCTTTAATTATTACTGCGAGAAGGTGGTGAAGGGGTTCTATAAGAATCATTTTCTGCGCTTTGATCGCCAGATTGTGCTGGTGGATTGCCTGCAACct                                                                                        >  1:1055433/2‑140 (MQ=255)                   cgcAGGCCGATAAGCATACCAATGCCGGAATGCTGCGCGAGCGCTTTAATTATTACTGCGAGAAGGTGGTGAAGGGGTTCTATAAGAATCATTTTCTTCGCTTTGATCGCCAGATTGTGCTGGTGGATTGCCTGCAACct                                                                                        >  2:1302115/1‑140 (MQ=255)                                  aTACCAATGCCGGAATGCTGCGCGAGCGCTTTAATTATTACTGCGAGAAGGTGGTGAAGGGGTTCTATAAGAATCATTTTCTGCGCTTTGATCGCCAGATTGTGCTGGTGGATTGCCTGCAACCTCTCAACAGTGGGCca                                                                         <  1:790453/140‑1 (MQ=255)                                  aTACCAATGCCGGAATGCTGCGCGAGCGCTTTAATTATTACTGCGAGAAGGTGGTGAAGGGGTTCTATAAGAATCATTTTCTGCGCTTTGATCGCCAGATTGTGCTGGTGGATTGCCTGCAACCTCTCAACAGTGGGCca                                                                         <  1:1261888/140‑1 (MQ=255)                                    aCCAATGCCGGAATGCTGCGCGAGCGCTTTAATTATTACTGCGAGAAGGTGGTGAAGGGGTTCTATAAGAATCATTTTCTGCGCTTTGATCGCCAGATTGTGCTGGTGGATTGCCTGCAACCTCTCAACAGTGGGCcaca                                                                       <  2:114546/140‑1 (MQ=255)                                     ccAATGCCGGAATGCTGCGCGAGCGCTTTAATTATTACTGCGAGAAGGTGGTGAAGGGGTTCTATAAGAATCATTTTCTGCGCTTTGATCGCCAGATTGTGCTGGTGGATTGCCTGCAACCTCTCAACAGTGGGCCACAg                                                                      <  1:1262601/140‑1 (MQ=255)                                         tGCCGGAATGCTGCGCGAGCGCTTTAATTATTACTGCGAGAAGGTGGTGAAGGGGTTCTATAAGAATCATTTTCTGCGCTTTGATCGCCAGATTGTGCTGGTGGATTGCCTGCAACCTCTCAACAGTGGGCCACAGGCAt                                                                  >  1:112059/1‑140 (MQ=255)                                          gCCGGAATGCTGCGCGAGCGCTTTAATTATTACTGCGAGAAGGTGGTGAAGGGGTTCTATAAGAATCATTTTCTGCGCTTTGATCGCCAGATTGTGCTGGTGGATTGCCTGCAACCTCTCAACAGTGGGc                                                                           <  1:930702/130‑1 (MQ=255)                                          gCCGGAATGCTGCGCGAGCGCTTTAATTATTACTGCGAGAAGGTGGTGAAGGGGTTCTATAAGAATCATTTTCTGCGCTTTGATCGCCAGATTGTGCTGGTGGATTGCCTGCAACCTCTCAACAGTGGGc                                                                           >  2:930702/1‑130 (MQ=255)                                          gCCGGAATGCTGCGCGAGCGCTTTAATTATTACTGCGAGAAGGTGGTGAAGGGGTTCTATAAGAATCATTTTCTGCGCTTTGATCGCCAGATTGTGCTGGTGGATTGCCTGCAACCTCTCAACAGTGGGCCACAGGCAtt                                                                 <  1:266326/140‑1 (MQ=255)                                          gCCGGAATGCTGCGCGAGCGCTTTAATTATTACTGCGAGAAGGTGGTGAAGGGGTTCTATAAGAATCATTTTCTGCGCTTTGATCGCCAGATTGTGCTGGTGGATTGCCTGCAACCTCTCAACAGTGGGCCACAGGCAtt                                                                 >  1:931510/1‑140 (MQ=255)                                            cGGAATGCTGCGCGAGCGCTTTAATTATTACTGCGAGAAGGTGGTGAAGGGGTTCTATAAGAATCATTTTCTGCGCTTTGATCGCCAGATTGTGCTGGTGGATTGCCTGCAACCTCTCAACAGTGGGCCACAGGCATTTa                                                               <  2:1305133/140‑1 (MQ=255)                                             ggAATGCTGCGCGAGCGCTTTAATTATTACTGCGAGAAGGTGGTGAAGGGGTTCTATAAGAATCATTTTCTGCGCTTTGATCGCCAGATTGTGCTGGTGGATTGCCTGCAACCTCTCAACAGTGGGCCACAGGCATTTaa                                                              <  2:1124263/140‑1 (MQ=255)                                              gAATGCTGCGCGAGCGCTTTAATTATTACTGCGAGAAGGTGGTGAAGGGGTTCTATAAGAATCATTTTCTGCGCTTTGATCGCCAGATTGTGCTGGTGGATTGCCTGCAACCTCTCAACAGTGGGCCACAGGCATTTAAt                                                             >  1:841422/1‑140 (MQ=255)                                              gAATGCTGCGCGAGCGCTTTAATTATTACTGCGAGAAGGTGGTGAAGGGGTTCTATAAGAATCATTTTCTGCGCTTTGATCGCCAGATTGTGCTGGTGGATTGCCTGCAACCTCTCAACAGTGGGCCACAGGCATTTAAt                                                             >  2:468583/1‑140 (MQ=255)                                               aaTGCTGCGCGAGCGCTTTAATTATTACTGCGAGAAGGTGGTGAAGGGGTTCTATAAGAATCATTTTCTGCGCTTTGATCGCCAGATTGTGCTGGTGGATTGCCTGCAACCTCTCAACAGTGGGCCACAGGCATTTAATg                                                            >  1:168975/1‑140 (MQ=255)                                                   ctgcGCGAGCGCTTTAATTATTACTGCGAGAAGGTGGTGAAGGGGTTCTATAAGAATCATTTTCTGCGCTTTGATCGCCAGATTGTGCTGGTGGATTGCCTGCAACCTCTCAACAGTGGGCCACAGGCATTTAATGatat                                                        <  2:112059/140‑1 (MQ=255)                                                     gcgcGAGCGCTTTAATTATTACTGCGAGAAGGTGGTGAAGGGGTTCTATAAGAATCATTTTCTGCGCTTTGATCGCCAGATTGTGCTGGTGGATTGCCTGCAACCTCTCAACAGTGGGCCACAGGCATTTAATGATATGc                                                      >  1:714211/1‑140 (MQ=255)                                                        cgAGCGCTTTAATTATTACTGCGAGAAGGTGGTGAAGGGGTTCTATAAGAATCATTTTCTGCGCTTTGATCGCCAGATTGTGCTGGTGGATTGCCTGCAACCTCTCAACAGTGGGCCACAGGCATTTAATGATATGCGTc                                                   <  2:57189/140‑1 (MQ=255)                                                                      attaCTGCGAGAAGGTGGTGAAGGGGTTCTATAAGAATCATTTTCTGCGCTTTGATCGCCAGATTGTGCTGGTGGATTGCCTGCAACCTCTCAACAGTGGGCCACAGGCATTTAATGATATGCGTCTGGCACTGACGCAg                                     <  2:168975/140‑1 (MQ=255)                                                                             cGAGAAGGTGGTGAAGGGGTTCTATAAGAATCATTTTCTGCGCTTTGATCGCCAGATTGTGCTGGTGGATTGCCTGCAACCTCTCAACAGTGGGCCACAGGCATTTAATGATATGCGTCTGGCACTGACGCAGCTGATGc                              >  2:221575/1‑140 (MQ=255)                                                                             cGAGAAGGTGGTGAAGGGGTTCTATAAGAATCATTTTCTGCGCTTTGATCGCCAGATTGTGCTGGTGGATTGCCTGCAACCTCTCAACAGTGGGCCACAGGCATTTAATGATATGCGTCTGGCACTGACGCAGCTGATGc                              >  2:1017099/1‑140 (MQ=255)                                                                             cGAGAAGGTGGTGAAGGGGTTCTATAAGAATCATTTTCTGCGCTTTGATCGCCAGATTGTGCTGGTGGATTGCCTGCAACCTCTCAACAGTGGGCCACAGGCATTTAATGATATGCGTCTGGCACTGACGCAGCTGATGc                              >  2:814751/1‑140 (MQ=255)                                                                             cGAGAAGGTGGTGAAGGGGTTCTATAAGAATCATTTTCTGCGCTTTGATCGCCAGATTGTGCTGGTGGATTGCCTGCAACCTCTCAACAGTGGGCCACAGGCATTTAATGATATGCGTCTGGCACTGACGCAGCTGATGc                              >  1:36066/1‑140 (MQ=255)                                                                               agaAGGTGGTGAAGGGGTTCTATAAGAATCATTTTCTGCGCTTTGATCGCCAGATTGTGCTGGTGGATTGCCTGCAACCTCTCAACAGTGGGCCACAGGCATTTAATGATATGCGTCTGGCACTGACGCAGCTGATGCaa                            >  2:148073/1‑140 (MQ=255)                                                                                    gtggtgAAGGGGTTCTATAAGAATCATTTTCTGCGCTTTGATCGCCAGATTGTGCTGGTGGATTGCCTGCAACCTCTCAACAGTGGGCCACAGGCATTTAATGATATGCGTCTGGCACTGACGCAGCTGATGCAAAGttt                       >  1:250305/1‑140 (MQ=255)                                                                                    gtggtgAAGGGGTTCTATAAGAATCATTTTCTGCGCTTTGATCGCCAGATTGTGCTGGTGGATTGCCTGCAACCTCTCAACAGTGGGCCACAGGCATTTAATGATATGCGTCTGGCACTGACGCAGCTGATGCAAAGttt                       <  2:931510/140‑1 (MQ=255)                                                                                         gAAGGGGTTCTATAAGAATCATTTTCTGCGCTTTGATCGCCAGATTGTGCTGGTGGATTGCCTGCAACCTCTCAACAGTGGGCCACAGGCATTTAATGATATGCGTCTGGCACTGACGCAGCTGATGCAAAGTTTTCACt                  <  2:1160377/140‑1 (MQ=255)                                                                                           aGGGGTTCTATAAGAATCATTTTCTGCGCTTTGATCGCCAGATTGTGCTGGTGGATTGCCTGCAACCTCTCAACAGTGGGCCACAGGCATTTAATGATATGCGTCTGGCACTGACGCAGCTGATGCAAAGTTTTCACTAc                >  1:1028031/1‑140 (MQ=255)                                                                                            ggggTTCTATAAGAATCATTTTCTGCGCTTTGATCGCCAGATTGTGCTGGTGGATTGCCTGCAACCTCTCAACAGTGGGCCACAGGCATTTAATGATATGCGTCTGGCACTGACGCAGCTGATGCAAAGTTTTCACTACg               >  2:2190/1‑140 (MQ=255)                                                                                              ggTTCTATAAGAATCATTTTCTGCGCTTTGATCGCCAGATTGTGCTGGTGGATTGCCTGCAACCTCTCAACAGTGGGCCACAGGCATTTAATGATATGCGTCTGGCACTGACGCAGCTGATGCAAAGTTTTCACTACggg             >  1:944808/1‑140 (MQ=255)                                                                                               gTTCTATAAGAATCATTTTCTGCGCTTTGATCGCCAGATTGTGCTGGTGGATTGCCTGCAACCTCTCAACAGTGGGCCACAGGCATTTAATGATATGCGTCTGGCACTGACGCAGCTGATGCAAAGTTTTCACTACGGGc            >  1:1345910/1‑140 (MQ=255)                                                                                               gTTCTATAAGAATCATTTTCTGCGCTTTGATCGCCAGATTGTGCTGGTGGATTGCCTGCAACCTCTCAACAGTGGGCCACAGGCATTTAATGATATGCGTCTGGCACTGACGCAGCTGATGCAAAGTTTTCACTACGGGc            >  1:1284352/1‑140 (MQ=255)                                                                                                  cTATAAGAATCATTTTCTGCGCTTTGATCGCCAGATTGTGCTGGTGGATTGCCTGCAACCTCTCAACAGTGGGCCACAGGCATTTAATGATATGCGTCTGGCACTGACGCAGCTGATGCAAAGTTTTCACTACGGGCAGc         >  2:1206847/1‑140 (MQ=255)                                                                                                  cTATAAGAATCATTTTCTGCGCTTTGATCGCCAGATTGTGCTGGTGGATTGCCTGCAACCTCTCAACAGTGGGCCACAGGCATTTAATGATATGCGTCTGGCACTGACGCAGCTGATGCAAAGTTTTCACTACGGGCAGc         >  2:1154172/1‑140 (MQ=255)                                                                                                  cTATAAGAATCATTTTCTGCGCTTTGATCGCCAGATTGTGCTGGTGGATTGCCTGCAACCTCTCAACAGTGGGCCACAGGCATTTAATGATATGCGTCTGGCACTGACGCAGCTGATGCAAAGTTTTCACTACGGGCAGc         <  2:1028031/140‑1 (MQ=255)                                                                                                       aGAATCATTTTCTGCGCTTTGATCGCCAGATTGTGCTGGTGGATTGCCTGCAACCTCTCAACAGTGGGCCACAGGCATTTAATGATATGCGTCTGGCACTGACGCAGCTGATGCAAAGTTTTCACTACGGGCAGCGTAcc    <  2:391650/140‑1 (MQ=255)                                                                                                       aGAATCATTTTCTGCGCTTTGATCGCCAGATTGTGCTGGTGGATTGCCTGCAACCTCTCAACAGTGGGCCACAGGCATTTAATGATATGCGTCTGGCACTGACGCAGCTGATGCAAAGTTTTCACTACGGGCAGCGTAcc    <  1:303992/140‑1 (MQ=255)                                                                                                         aaTCATTTTCTGCGCTTTGATCGCCAGATTGTGCTGGTGGATTGCCTGCAACCTCTCAACAGTGGGCCACAGGCATTTAATGATATGCGTCTGGCACTGACGCAGCTGATGCAAAGTTTTCACTACGGGCAGCGTACCCt  >  2:154725/1‑140 (MQ=255)                                                                                                                             |                                                                                                                        GGGGCGAGTCCAAACTGGCGCAGGCCGATAAGCATACCAATGCCGGAATGCTGCGCGAGCGCTTTAATTATTACTGCGAGAAGGTGGTGAAGGGGTTCTATAAGAATCATTTTCTGCGCTTTGACCGCCAGATTGTGCTGGTGGATTGCCTGCAACCTCTCAACAGTGGGCCACAGGCATTTAATGATATGCGTCTGGCACTGACGCAGCTGATGCAAAGTTTTCACTACGGGCAGCGTACCCT  >  NC_000913/1384832‑1385075

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑