Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,403,596	T→C	T324A (ACC→GCC)	abgA ←	p‑aminobenzoyl‑glutamate hydrolase subunit A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,403,596	0	T	C	100.0%	149.6 / NA	44	T324A (ACC→GCC)	abgA	p‑aminobenzoyl‑glutamate hydrolase subunit A
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (20/24);  total (20/24)

CGTTCAATGGCCTGATTGACCCCCGCGACCTGAGCCGCCTGACTTTGCAACCATGCGACCCATTGCGGCGAAGGAGAACTGGCGGTAGCTGCACCCATCAGACGAGTTTCAACGCCGACACCATACATGGTTGCTGCGCCCTGAATCGCTTGTTGTGCACGGTCAAAAACATATTGATTAATGACGTCGCTGGCCCCGCGTGTTTCCACTTTCAGCAACGCCGAGGCAGGAACAACGTTACGACCGCTTCCTGCCTGCATAAC  >  NC_000913/1403466‑1403728                                                                                                                                   |                                                                                                                                     cGTTCAATGGCCTGATTGACCCCCGCGACCTGAGCCGCCTGACTTTGCAACCATGCGACCCATTGCGGCGAAGGAGAACTGGCGGTAGCTGCACCCATCAGACGAGTTTCAACGCCGACACCATACATGGCTGCTGCGcc                                                                                                                             <  1:1113250/140‑1 (MQ=255) cGTTCAATGGCCTGATTGACCCCCGCGACCTGAGCCGCCTGACTTTGCAACCATGCGACCCATTGCGGCGAAGGAGAACTGGCGGTAGCTGCACCCATCAGACGAGTTTCAACGCCGACACCATACATGGCTGCTGCGcc                                                                                                                             >  2:246915/1‑140 (MQ=255)     cAATGGCCTGATTGACCCCCGCGACCTGAGCCGCCTGACTTTGCAACCATGCGACCCATTGCGGCGAAGGAGAACTGGCGGTAGCTGCACCCATCAGACGAGTTTCAACGCCGACACCATACATGGCTGCTGCGCCCTGa                                                                                                                         <  1:641211/140‑1 (MQ=255)     cAATGGCCTGATTGACCCCCGCGACCTGAGCCGCCTGACTTTGCAACCATGCGACCCATTGCGGCGAAGGAGAACTGGCGGTAGCTGCACCCATCAGACGAGTTTCAACGCCGACACCATACATGGCTGCTGCGCCCTGa                                                                                                                         <  2:564407/140‑1 (MQ=255)      aaTGGCCTGATTGACCCCCGCGACCTGAGCCGCCTGACTTTGCAACCATGCGACCCATTGCGGCGAAGGAGAACTGGCGGTAGCTGCACCCATCAGACGAGTTTCAACGCCGACACCATACATGGCTGCTGCGCCCTGaa                                                                                                                        >  2:1210727/1‑140 (MQ=255)          gCCTGATTGACCCCCGCGACCTGAGCCGCCTGACTTTGCAACCATGCGACCCATTGCGGCGAAGGAGAACTGGCGGTAGCTGCACCCATCAGACGAGTTTCAACGCCGACACCATACATGGCTGCTGCGCCCTGAATCGc                                                                                                                    <  1:1210727/140‑1 (MQ=255)            cTGATTGACCCCCGCGACCTGAGCCGCCTGACTTTGCAACCATGCGACCCATTGCGGCGAAGGAGAACTGGCGGTAGCTGCACCCATCAGACGAGTTTCAACGCCGACACCATACATGGCTGCTGCGCCCTGAATCGCtt                                                                                                                  >  1:626434/1‑140 (MQ=255)             tGATTGACCCCCGCGACCTGAGCCGCCTGACTTTGCAACCATGCGACCCATTGCGGCGAAGGAGAACTGGCGGTAGCTGCACCCATCAGACGAGTTTCAACGCCGACACCATACATGGCTGCTGCGCCCTGAATCGCttg                                                                                                                 <  1:753347/140‑1 (MQ=255)             tGATTGACCCCCGCGACCTGAGCCGCCTGACTTTGCAACCATGCGACCCATTGCGGCGAAGGAGAACTGGCGGTAGCTGCACCCATCAGACGAGTTTCAACGCCGACACCATACATGGCCGCTGCGCCCTGAATCGCttg                                                                                                                 <  2:685002/140‑1 (MQ=255)                  gACCCCCGCGACCTGAGCCGCCTGACTTTGCAACCATGCGACCCATTGCGGCGAAGGAGAACTGGCGGTAGCTGCACCCATCAGACGAGTTTCAACGCCGACACCATACATGGCTGCTGCGCCCTGAATCGCTTGTtgtg                                                                                                            <  1:558168/140‑1 (MQ=255)                    cccccGCGACCTGAGCCGCCTGACTTTGCAACCATGCGACCCATTGCGGCGAAGGAGAACTGGCGGTAGCTGCACCCATCAGACGAGTTTCAACGCCGACACCATACATGGCTGCTGCGCCCTGAATCGCTTGTTGTGCa                                                                                                          >  2:810497/1‑140 (MQ=255)                        cgcgACCTGAGCCGCCTGACTTTGCAACCATGCGACCCATTGCGGCGAAGGAGAACTGGCGGTAGCTGCACCCATCAGACGAGTTTCAACGCCGACACCATACATGGCTGCTGCGCCCTGAATCGCTTGTTGTGCACGGt                                                                                                      >  2:1329098/1‑140 (MQ=255)                         gcgACCTGAGCCGCCTGACTTTGCAACCATGCGACCCATTGCGGCGAAGGAGAACTGGCGGTAGCTGCACCCATCAGACGAGTTTCAACGCCGACACCATACATGGCTGCTGCGCCCTGAATCGCTTGTTGTGCa                                                                                                          >  2:1141465/1‑135 (MQ=255)                         gcgACCTGAGCCGCCTGACTTTGCAACCATGCGACCCATTGCGGCGAAGGAGAACTGGCGGTAGCTGCACCCATCAGACGAGTTTCAACGCCGACACCATACATGGCTGCTGCGCCCTGAATCGCTTGTTGTGCa                                                                                                          <  1:1141465/135‑1 (MQ=255)                         gcgACCTGAGCCGCCTGACTTTGCAACCATGCGACCCATTGCGGCGAAGGAGAACTGGCGGTAGCTGCACCCATCAGACGAGTTTCAACGCCGACACCATACATGGCTGCTGCGCCCTGAATCGCTTGTTGTGCACggcc                                                                                                     >  2:551639/1‑138 (MQ=255)                                 aGCCGCCTGACTTTGCAACCATGCGACCCATTGCGGCGAAGGAGAACTGGCGGTAGCTGCACCCATCAGACGAGTTTCAACGCCGACACCATACATGGCTGCTGCGCCCTGAATCGCTTGTTGTGCACGGTCAAAAACat                                                                                             >  1:516802/1‑140 (MQ=255)                                  gccgccTGACTTTGCAACCATGCGACCCATTGCGGCGAAGGAGAACTGGCGGTAGCTGCACCCATCAGACGAGTTTCAACGCCGACACCATACATGGCTGCTGCGCCCTGAATCGCTTGTTGTGCACGGTCAAAAACata                                                                                            <  1:500599/140‑1 (MQ=255)                                  gccgccTGACTTTGCAACCATGCGACCCATTGCGGCGAAGGAGAACTGGCGGTAGCTGCACCCATCAGACGAGTTTCAACGCCGACACCATACATGGCTGCTGCGCCCTGAATCGCTTGTTGTGCACGGTCAAAAACata                                                                                            >  1:1121403/1‑140 (MQ=255)                                   ccgccTGACTTTGCAACCATGCGACCCATTGCGGCGAAGGAGAACTGGCGGTAGCTGCACCCATCAGACGAGTTTCAACGCCGACACCATACATGGCTGCTGCGCCCTGAATCGCTTGTTGTGCACGGTCAAAAACatat                                                                                           <  1:89609/140‑1 (MQ=255)                                   ccgccTGACTTTGCAACCATGCGACCCATTGCGGCGAAGGAGAACTGGCGGTAGCTGCACCCATCAGACGAGTTTCAACGCCGACACCATACATGGCTGCTGCGCCCTGAATCGCTTGTTGTGCACGGTCAAAAACatat                                                                                           <  1:1081654/140‑1 (MQ=255)                                    cgccTGACTTTGCAACCATGCGACCCATTGCGGCGAAGGAGAACTGGCGGTAGCTGCACCCATCAGACGAGTTTCAACGCCGACACCATACATGGCTGCTGCGCCCTGAATCGCTTGTTGTGCACGGTCAAAAACATAtt                                                                                          <  1:625977/140‑1 (MQ=255)                                      ccTGACTTTGCAACCATGCGACCCATTGCGGCGAAGGAGAACTGGCGGTAGCTGCACCCATCAGACGAGTTTCAACGCCGACACCATACATGGCTGCTGCGCCCTGAATCGCTTGTTGTGCACGGTCAAAAACATATTGa                                                                                        >  1:650424/1‑140 (MQ=255)                                                 aaCCATGCGACCCATTGCGGCGAAGGAGAACTGGCGGTAGCTGCACCCATCAGACGAGTTTCAACGCCGACACCATACATGGCTGCTGCGCCCTGAATCGCTTGTTGTGCACGGTCAAAAACATATTGATTAATGACGTc                                                                             >  1:952708/1‑140 (MQ=255)                                                     aTGCGACCCATTGCGGCGGAGGAGAACTGGCGGTAGCTGCACCCATCAGACGAGTTTCAACGCCGACACCATACATGGCTGCTGCGCCCTGAATCGCTTGTTGTGCACGGTCAAAAACATATTGATTAATGAc                                                                                >  2:638907/1‑133 (MQ=255)                                                     aTGCGACCCATTGCGGCGGAGGAGAACTGGCGGTAGCTGCACCCATCAGACGAGTTTCAACGCCGACACCATACATGGCTGCTGCGCCCTGAATCGCTTGTTGTGCACGGTCAAAAACATATTGATTAATGAc                                                                                <  1:638907/133‑1 (MQ=255)                                                     aTGCGACCCATTGCGGCGAAGGAGAACTGGCGGTAGCTGCACCCATCAGACGAGTTTCAACGCCGACACCATACATGGCTGCTGCGCCCTGAATCGCTTGTTGTGCACGGTCAAAAACATATTGATTAATGACGTCGCTg                                                                         >  1:1373230/1‑140 (MQ=255)                                                             cATTGCGGCGAAGGAGAACTGGCGGTAGCTGCACCCATCAGACGAGTTTCAACGCCGACACCATACATGGCTGCTGCGCCCTGAATCGCTTGTTGTGCACGGTCAAAAACATATTGATTAATGACGTCGCTGGCCCcgcg                                                                 >  1:415179/1‑140 (MQ=255)                                                                                    ggTAGCTGCACCCATCAGACGAGTTTCAACGCCGACACCATACATGGCTGCTGCGCCCTGAATCGCTTGTTGTGCACGGTCAAAAACATATTGATTAATGACGTCGCTGGCCCCGCGTGTTTCCACTTTCAGCAACGCCg                                          >  1:424645/1‑140 (MQ=255)                                                                                       aGCTGCACCCATCAGACGAGTTTCAACGCCGACACCATACATGGCTGCTGCGCCCTGAATCGCTTGTTGTGCACGGTCAAAAACATATTGATTAATGACGTCGCTGGCCCCGCGTGTTTCCACTTTCAGCAACGCCGAgg                                       <  1:1329098/140‑1 (MQ=255)                                                                                       aGCTGCACCCATCAGACGAGTTTCAACGCCGACACCATACATGGCTGCTGCGCCCTGAATCGCTTGTTGTGCACGGCCAAAAACATATTGATTAATGACGTCGCTGGCCCCGCGTGTTTCCACTTTCAGCAACGCCGAgg                                       <  1:551639/140‑1 (MQ=255)                                                                                            cACCCATCAGACGAGTTTCAACGCCGACACCATACATGGCTGCTGCGCCCTGAATCGCTTGTTGTGCACGGTCAAAAACATATTGATTAATGACGTCGCTGGCCCCGCGTGTTTCCACTTTCAGCAACGcc                                           <  2:799270/131‑1 (MQ=255)                                                                                            cACCCATCAGACGAGTTTCAACGCCGACACCATACATGGCTGCTGCGCCCTGAATCGCTTGTTGTGCACGGTCAAAAACATATTGATTAATGACGTCGCTGGCCCCGCGTGTTTCCACTTTCAGCAACGcc                                           >  1:799270/1‑131 (MQ=255)                                                                                            cACCCATCAGACGAGTTTCAACGCCGACACCATACATGGCTGCTGCGCCCTGAATCGCTTGTTGTGCACGGTCAAAAACATATTGATTAATGACGTCGCTGGCCCCGCGTGTTTCCACTTTCAGCAACGCCGAGGCAGGa                                  <  2:703932/140‑1 (MQ=255)                                                                                              cccATCAGACGAGTTTCAACGCCGACACCATACATGGCTGCTGCGCCCTGAATCGCTTGTTGTGCACGGTCAAAAACATATTGATTAATGACGTCGCTGGCGCCGCGTGTTTCCCCTTTCAGCAGCGCCGAGGCAGGaac                                <  2:1170793/140‑1 (MQ=255)                                                                                              cccATCAGACGAGTTTCAACGCCGACACCATACATGGCTGCTGCGCCCTGAATCGCTTGTTGTGCACGGTCAAAAACATATTGATTAATGACGTCGCTGGCCCCGCGTGTTTCCACTTTCAGCAACGCCGAGGCAGGaac                                <  1:390168/140‑1 (MQ=255)                                                                                                 aTCAGACGAGTTTCAACGCCGACACCATACATGGCTGCTGCGCCCTGAATCGCTTGTTGTGCACGGTCAAAAACATATTGATTAATGACGTCGCTGGCCCCGCGTGTTTCCACTTTCAGCAACGCCGAGGCAGGaacaac                             >  2:1160369/1‑140 (MQ=255)                                                                                                 aTCAGACGAGTTTCAACGCCGACACCATACATGGCTGCTGCGCCCTGAATCGCTTGTTGTGCACGGTCAAAAACATATTGATTAATGACGTCGCTGGCCCCGCGTGTTTCCACTTTCAGCAACGCCGAGGCAGGaacaac                             >  1:199175/1‑140 (MQ=255)                                                                                                 aTCAGACGAGTTTCAACGCCGACACCATACATGGCTGCTGCGCCCTGAATCGCTTGTTGTGCACGGTCAAAAACATATTGATTAATGACGTCGCTGGCCCCGCGTGTTTCCACTTTCAGCAACGCCGAGGCAGGaac                                >  1:1043759/1‑137 (MQ=255)                                                                                                 aTCAGACGAGTTTCAACGCCGACACCATACATGGCTGCTGCGCCCTGAATCGCTTGTTGTGCACGGTCAAAAACATATTGATTAATGACGTCGCTGGCCCCGCGTGTTTCCACTTTCAGCAACGCCGAGGCAGGaac                                <  2:1043759/137‑1 (MQ=255)                                                                                                         ggTTTCAACGCCGACACCATACATGGCTGCTGCGCCCTGAATCGCTTGTTGTGCACGGTCAAAAACATATTGATTAATGACGTCGCTGGCCCCGCGTGTTTCCACTTTCAGCAACGCCGAGGCAGGAACAACGTTACGAc                     <  2:1121403/139‑1 (MQ=255)                                                                                                         aGTTTCAACGCCGACACCATACATGGCTGCTGCGCCCTGAATCGCTTGTTGTGCACGGTCAAAAACATATTGATTAATGACGTCGCTGGCCCCGCGTGTTTCCACTTTCAGCAACGCCGAGGCAGGAACAACGTTACGAc                     <  2:516802/140‑1 (MQ=255)                                                                                                         aGTTTCAACGCCGACACCATACATGGCTGCTGCGCCCTGAATCGCTTGTTGTGCACGGTCAAAAACATATTGATTAATGACGTCGCTGGCCCCGCGTGTTTCCACTTTCAGCAACGCCGAGGCAGGAACAACGTTACGAc                     <  2:415179/140‑1 (MQ=255)                                                                                                         aGTTTCAACGCCGACACCATACATGGCTGCTGCGCCCTGAATCGCTTGTTGTGCACGGTCAAAAACATATTGATTAATGACGTCGCTGGCCCCGCGTGTTTCCACTTTCAGCAACGCCGAGGCAGGAACAACGTTACGAc                     <  1:1160369/140‑1 (MQ=255)                                                                                                           tttCAACGCCGACACCATACATGGCTGCTGCGCCCTGAATCGCTTGTTGTGCACGGTCAAAAACATATTGATTAATGACGTCGCTGGCCCCGCGTGTTTCCACTTTCAGCAACGCCGAGGCAGGAACAACGTTACGACCg                   >  1:798352/1‑140 (MQ=255)                                                                                                              cAACGCCGACACCATACATGGCTGCTGCGCCCTGAATCGCTTGTTGTGCACGGTCAAAAACATATTGATTAATGACGTCGCTGGCCCCGCGTGTTTCCACTTTCAGCAACGCCGAGGCAGGAACAACGTTACGACCGCtt                <  2:1084594/140‑1 (MQ=255)                                                                                                                            tACATGGCTGCTGCGCCCTGAATCGCTTGTTGTGCACGGTCAAAAACATATTGATTAATGACGTCGCTGGCCCCGCGTGTTTCCACTTTCAGCAACGCCGAGGCAGGAACAACGTTACGACCGCTTCCTGCCTGCATAAc  >  2:1375262/1‑140 (MQ=255)                                                                                                                                   |                                                                                                                                     CGTTCAATGGCCTGATTGACCCCCGCGACCTGAGCCGCCTGACTTTGCAACCATGCGACCCATTGCGGCGAAGGAGAACTGGCGGTAGCTGCACCCATCAGACGAGTTTCAACGCCGACACCATACATGGTTGCTGCGCCCTGAATCGCTTGTTGTGCACGGTCAAAAACATATTGATTAATGACGTCGCTGGCCCCGCGTGTTTCCACTTTCAGCAACGCCGAGGCAGGAACAACGTTACGACCGCTTCCTGCCTGCATAAC  >  NC_000913/1403466‑1403728

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑