Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	191,337	G→A	A161T (GCT→ACT)	tsf →	protein chain elongation factor EF‑Ts

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	191,337	0	G	A	100.0%	169.5 / NA	48	A161T (GCT→ACT)	tsf	protein chain elongation factor EF‑Ts
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (26/22);  total (26/22)

AACGTGTTGCGCTGGTAGCGAAAATTGGTGAAAACATCAACATTCGCCGCGTTGCTGCGCTGGAAGGCGACGTTCTGGGTTCTTATCAGCACGGTGCGCGTATCGGCGTTCTGGTTGCTGCTAAAGGCGCTGACGAAGAGCTGGTTAAACACATCGCTATGCACGTTGCTGCAAGCAAGCCAGAATTCATCAAACCGGAAGACGTATCCGCTGAAGTGGTAGAAAAAGAATACCAGGTACAGCTGGATATCGCGATGCAGTCT  >  NC_000913/191209‑191471                                                                                                                                 |                                                                                                                                       aaCGTGTTGCGCTGGTAGCGAAAATTGGTGAAAACATCAACATTCGCCGCGTTGCTGCGCTGGAAGGCGACGTTCTGGGTTCTTATCAGCACGGTGCGCGTATCGGCGTTCTGGTTGCTGCTAAAGGCACTGACGAagag                                                                                                                             >  1:470081/1‑140 (MQ=255)  aCGTGTTGCGCTGGTAGCGAAAATTGGTGAAAACATCAACATTCGCCGCGTTGCTGCGCTGGAAGGCGACGTTCTGGGTTCTTATCAGCACGGTGCGCGTATCGGCGTTCTGGTTGCTGCTAAAGGCACTGACGAAGAGc                                                                                                                            <  1:568383/140‑1 (MQ=255)  aCGTGTTGCGCTGGTAGCGAAAATTGGTGAAAACATCAACATTCGCCGCGTTGCTGCGCTGGAAGGCGACGTTCTGGGTTCTTATCAGCACGGTGCGCGTATCGGCGTTCTGGTTGCTGCTAAAGGCACTGACGAAGAGc                                                                                                                            <  2:636741/140‑1 (MQ=255)     tgtTGCGCTGGTAGCGAAAATTGGTGAAAACATCAACATTCGCCGCGTTGCTGCGCTGGAAGGCGACGTTCTGGGTTCTTATCAGCACGGTGCGCGTATCGGCGTTCTGGTTGCTGCTAAAGGCACTGACGAAGAGCTgg                                                                                                                         >  2:378294/1‑140 (MQ=255)     tgtTGCGCTGGTAGCGAAAATTGGTGAAAACATCAACATTCGCCGCGTTGCTGCGCTGGAAGGCGACGTTCTGGGTTCTTATCAGCACGGTGCGCGTATCGGCGTTCTGGTTGCTGCTAAAGGCACTGACGAAGAGCTgg                                                                                                                         <  1:162666/140‑1 (MQ=255)              ggTAGCGAAAATTGGTGAAAACATCAACATTCGCCGCGTTGCTGCGCTGGAAGGCGACGTTCTGGGTTCTTATCAGCACGGTGCGCGTATCGGCGTTCTGGTTGCTGCTAAAGGCACTGACGAAGAGCTGGTTAAAcaca                                                                                                                <  2:470081/140‑1 (MQ=255)               gTAGCGAAAATTGGTGAAAACATCAACATTCGCCGCGTTGCTGCGCTGGAAGGCGACGTTCTGGGTTCTTATCAGCACGGTGCGCGTATCGGCGTTCTGGTTGCTGCTAAAGGCACTGACGAAGAGCTGGTTAAACACAt                                                                                                               >  2:44283/1‑140 (MQ=255)               gTAGCGAAAATTGGTGAAAACATCAACATTCGCCGCGTTGCTGCGCTGGAAGGCGACGTTCTGGGTTCTTATCAGCACGGTGCGCGTATCGGCGTTCTGGTTGCTGCTAAAGGCACTGACGAAGAGCTGGTTAAACACAt                                                                                                               >  1:218504/1‑140 (MQ=255)               gTAGCGAAAATTGGTGAAAACATCAACATTCGCCGCGTTGCTGCGCTGGAAGGCGACGTTCTGGGTTCTTATCAGCACGGTGCGCGTATCGGCGTTCTGGTTGCTGCTAAAGGCACTGACGAAGAGCTGGTTAAACACAt                                                                                                               >  2:668100/1‑140 (MQ=255)                    gAAAATTGGTGAAAACATCAACATTCGCCGCGTTGCTGCGCTGGAAGGCGACGTTCTGGGTTCTTATCAGCACGGTGCGCGTATCGGCGTTCTGGTTGCTGCTAAAGGCACTGACGAAGAGCTGGTTAAACACATCGCTa                                                                                                          >  2:204358/1‑140 (MQ=255)                        aTTGGTGAAAACATCAACATTCGCCGCGTTGCTGCGCTGGAAGGCGACGTTCTGGGTTCTTATCAGCACGGTGCGCGTATCGGCGTTCTGGTTGCTGCTAAAGGCACTGACGAAGAGCTGGTTAAACACATCGCTATGCa                                                                                                      <  1:668100/140‑1 (MQ=255)                           ggTGAAAACATCAACATTCGCCGCGTTGCTGCGCTGGAAGGCGACGTTCTGGGTTCTTATCAGCACGGTGCGCGTATCGGCGTTCTGGTTGCTGCTAAAGGCACTGACGAAGAGCTGGTTAAACACATCGCTATGCACGt                                                                                                   <  2:1250401/140‑1 (MQ=255)                                    aTCAACATTCGCCGCGTTGCTGCGCTGGAAGGCGACGTTCTGGGTTCTTATCAGCACGGTGCGCGTATCGGCGTTCTGGTTGCTGCTAAAGGCACTGACGAAGAGCTGGTTAAACACATCGCTATGCACGTTGCTgcaag                                                                                          <  1:488176/140‑1 (MQ=255)                                    aTCAACATTCGCCGCGTTGCTGCGCTGGAAGGCGACGTTCTGGGTTCTTATCAGCACGGTGCGCGTATCGGCGTTCTGGTTGCTGCTAAAGGCACTGACGAAGAGCTGGTTAAACACATCGCTATGCACGTTGCTgcaag                                                                                          <  1:1121666/140‑1 (MQ=255)                                    aTCAACATTCGCCGCGTTGCTGCGCTGGAAGGCGACGTTCTGGGTTCTTATCAGCACGGTGCGCGTATCGGCGTTCTGGTTGCTGCTAAAGGCACTGACGAAGAGCTGGTTAAACACATCGCTATGCACGTTGCTgcaag                                                                                          <  1:851876/140‑1 (MQ=255)                                    aTCAACATTCGCCGCGTTGCTGCGCTGGAAGGCGACGTTCTGGGTTCTTATCAGCACGGTGCGCGTATCGGCGTTCTGGTTGCTGCTAAAGGCACTGACGAAGAGCTGGTTAAACACATCGCTATGCACGTTGCTgcaag                                                                                          <  1:243244/140‑1 (MQ=255)                                              gccgcGTTGCTGCGCTGGAAGGCGACGTTCTGGGTTCTTATCAGCACGGTGCGCGTATCGGCGTTCTGGTTGCTGCTAAAGGCACTGACGAAGAGCTGGTTAAACACATCGCTATGCACGTTGCTGCAAGCAAGCCAGaa                                                                                >  1:913550/1‑140 (MQ=255)                                                     tgctgcGCTGGAAGGCGACGTTCTGGGTTCTTATCAGCACGGTGCGCGTATCGGCGTTCTGGTTGCTGCTAAAGGCACTGACGAAGAGCTGGTTAAACACATCGCTATGCACGTTGCTGCAAGCAAGCCAGAATtcatca                                                                         <  1:281171/140‑1 (MQ=255)                                                       ctgcGCTGGAAGGCGACGTTCTGGGTTCTTATCAGCACGGTGCGCGTATCGGCGTTCTGGTTGCTGCTAAAGGCACTGACGAAGAGCTGGTTAAACACATCGCTATGCACGTTGCTGCAAGCAAGCCAGAATTCATCaaa                                                                       <  1:378294/140‑1 (MQ=255)                                                                     gACGTTCTGGGTTCTTATCAGCACGGTGCGCGTATCGGCGTTCTGGTTGCTGCTAAAGGCACTGACGAAGAGCTGGTTAAACACATCGCTATGCACGTTGCTGCAAGCAAGCCAGAATTCATCAAACCGGAAGACGTATc                                                         <  1:204358/140‑1 (MQ=255)                                                                                        aGCACGGTGCGCGTATCGGCGTTCTGGTTGCTGCTAAAGGCACTGACGAAGAGCTGGTTAAACACATCGCTATGCACGTTGCTGCAAGCAAGCCAGAATTCATCAAACCGGAAGACGTATCCGCTGAAGTGGTAGaaaaa                                      >  1:398049/1‑140 (MQ=255)                                                                                        aGCACGGTGCGCGTATCGGCGTTCTGGTTGCTGCTAAAGGCACTGACGAAGAGCTGGTTAAACACATCGCTATGCACGTTGCTGCAAGCAAGCCAGAATTCATCAAACCGGAAGACGTATCCGCTGAAGTGGTAGaaaaa                                      >  1:505915/1‑140 (MQ=255)                                                                                        aGCACGGTGCGCGTATCGGCGTTCTGGTTGCTGCTAAAGGCACTGACGAAGAGCTGGTTAAACACATCGCTATGCACGTTGCTGCAAGCAAGCCAGAATTCATCAAACCGGAAGACGTATCCGCTGAAGTGGTAGaaaaa                                      <  2:505915/140‑1 (MQ=255)                                                                                          cACGGTGCGCGTATCGGCGTTCTGGTTGCTGCTAAAGGCACTGACGAAGAGCTGGTTAAACACATCGCTATGCACGTTGCTGCAAGCAAGCCAGAATTCATCAAACCGGAAGACGTATCCGCTGAAGTGGTAGAAAAAGa                                    <  2:913550/140‑1 (MQ=255)                                                                                          cACGGTGCGCGTATCGGCGTTCTGGTTGCTGCTAAAGGCACTGACGAAGAGCTGGTTAAACACATCGCTATGCACGTTGCTGCAAGCAAGCCAGAATTCATCAAACCGGAAGACGTATCCGCTGAAGTGGTAGAAAAAGa                                    >  2:495272/1‑140 (MQ=255)                                                                                             ggTGCGCGTATCGGCGTTCTGGTTGCTGCTAAAGGCACTGACGAAGAGCTGGTTAAACACATCGCTATGCACGTTGCTGCAAGCAAGCCAGAATTCATCAAACCGGAAGACGTATCCGCTGAAGTGGTAGAAAAAGAATa                                 >  2:803144/1‑140 (MQ=255)                                                                                             ggTGCGCGTATCGGCGTTCTGGTTGCTGCTAAAGGCACTGACGAAGAGCTGGTTAAACACATCGCTATGCACGTTGCTGCAAGCAAGCCAGAATTCATCAAACCGGAAGACGTATCCGCTGAAGTGGTAGAAAAAGAATa                                 <  1:803144/140‑1 (MQ=255)                                                                                             ggTGCGCGTATCGGCGTTCTGGTTGCTGCTAAAGGCACTGACGAAGAGCTGGTTAAACACATCGCTATGCACGTTGCTGCAAGCAAGCCAGAATTCATCAAACCGGAAGACGTATCCGCTGAAGTGGTAGAAAAAGAATa                                 >  2:285372/1‑140 (MQ=255)                                                                                               tGCGCGTATCGGCGTTCTGGTTGCTGCTAAAGGCACTGACGAAGAGCTGGTTAAACACATCGCTATGCACGTTGCTGCAAGCAAGCCAGAATTCATCAAACCGGAAGACGTATCCGCTGAAGTGGTAGAAAAAGAATAcc                               <  2:814271/140‑1 (MQ=255)                                                                                               tGCGCGTATCGGCGTTCTGGTTGCTGCTAAAGGCACTGACGAAGAGCTGGTTAAACACATCGCTATGCACGTTGCTGCAAGCAAGCCAGAATTCATCAAACCGGAAGACGTATCCGCTGAAGTGGTAGAAAAAGAATAcc                               >  1:814271/1‑140 (MQ=255)                                                                                               tGCGCGTATCGGCGTTCTGGTTGCTGCTAAAGGCACTGACGAAGAGCTGGTTAAACACATCGCTATGCACGTTGCTGCAAGCAAGCCAGAATTCATCAAACCGGAAGACGTATCCGCTGAAGTGGTAGAAAAAGAATAcc                               >  1:1327349/1‑140 (MQ=255)                                                                                               tGCGCGTATCGGCGTTCTGGTTGCTGCTAAAGGCACTGACGAAGAGCTGGTTAAACACATCGCTATGCACGTTGCTGCAAGCAAGCCAGAATTCATCAAACCGGAAGACGTATCCGCTGAAGTGGTAGAAAAAGAATAcc                               >  1:260224/1‑140 (MQ=255)                                                                                               tGCGCGTATCGGCGTTCTGGTTGCTGCTAAAGGCACTGACGAAGAGCTGGTTAAACACATCGCTATGCACGTTGCTGCAAGCAAGCCAGAATTCATCAAACCGGAAGACGTATCCGCTGAAGTGGTAGAAAAAGAATAcc                               >  1:880826/1‑140 (MQ=255)                                                                                               tGCGCGTATCGGCGTTCTGGTTGCTGCTAAAGGCACTGACGAAGAGCTGGTTAAACACATCGCTATGCACGTTGCTGCAAGCAAGCCAGAATTCATCAAACCGGAAGACGTATCCGCTGAAGTGGTAGAAAAAGAATAcc                               >  1:857168/1‑140 (MQ=255)                                                                                               tGCGCGTATCGGCGTTCTGGTTGCTGCTAAAGGCACTGACGAAGAGCTGGTTAAACACATCGCTATGCACGTTGCTGCAAGCAAGCCAGAATTCATCAAACCGGAAGACGTATCCGCTGAAGTGGTAGAAAAAGAATAcc                               >  1:53889/1‑140 (MQ=255)                                                                                                  gcgTATCGGCGTTCTGGTTGCTGCTAAAGGCACTGACGAAGAGCTGGTTAAACACATCGCTATGCACGTTGCTGCAAGCAAGCCAGAATTCATCAAACCGGAAGACGTATCCGCTGAAGTGGTAGAAAAAGAATACCAgg                            <  1:795550/140‑1 (MQ=255)                                                                                                    gTATCGGCGTTCTGGTTGCTGCTAAAGGCACTGACGAAGAGCTGGTTAAACACATCGCTATGCACGTTGCTGCAAGCAAGCCAGAATTCATCAAACCGGAAGACGTATCCGCTGAAGTGGTAGAAAAAGAATACCAGGTa                          <  1:495272/140‑1 (MQ=255)                                                                                                         ggCGTTCTGGTTGCTGCTAAAGGCACTGACGAAGAGCTGGTTAAACACATCGCTATGCACGTTGCTGCAAGCAAGCCAGAATTCATCAAACCGGAAGACGTATCCGCTGAAGTGGTAGAAAAAGAATACCAGGTACAGCt                     >  1:925235/1‑140 (MQ=255)                                                                                                         ggCGTTCTGGTTGCTGCTAAAGGCACTGACGAAGAGCTGGTTAAACACATCGCTATGCACGTTGCTGCAAGCAAGCCAGAATTCATCAAACCGGAAGACGTATCCGCTGAAGTGGTAGAAAAAGAATACCAGGTACAGCt                     >  1:1022492/1‑140 (MQ=255)                                                                                                           cGTTCTGGTTGCTGCTAAAGGCACTGACGAAGAGCTGGTTAAACACATCGCTATGCACGTTGCTGCAAGCAAGCCAGAATTCATCAAACCGGAAGACGTATCCGCTGAAGTGGTAGAAAAAGAATACCAGGTACAGCTgg                   >  2:419951/1‑140 (MQ=255)                                                                                                           cGTTCTGGTTGCTGCTAAAGGCACTGACGAAGAGCTGGTTAAACACATCGCTATGCACGTTGCTGCAAGCAAGCCAGAATTCATCAAACCGGAAGACGTATCCGCTGAAGTGGTAGAAAAAGAATACCAGGTACAGCTgg                   >  2:952477/1‑140 (MQ=255)                                                                                                             ttCTGGTTGCTGCTAAAGGCACTGACGAAGAGCTGGTTAAACACATCGCTATGCACGTTGCTGCAAGCAAGCCAGAATTCATCAAACCGGAAGACGTATCCGCTGAAGTGGTAGAAAAAGAATACCAgg                            >  2:380418/1‑129 (MQ=255)                                                                                                             ttCTGGTTGCTGCTAAAGGCACTGACGAAGAGCTGGTTAAACACATCGCTATGCACGTTGCTGCAAGCAAGCCAGAATTCATCAAACCGGAAGACGTATCCGCTGAAGTGGTAGAAAAAGAATACCAgg                            <  1:380418/129‑1 (MQ=255)                                                                                                              tCTGGTTGCTGCTAAAGGCACTGACGAAGAGCTGGTTAAACACATCGCTATGCACGTTGCTGCAAGCAAGCCAGAATTCATCAAACCGGAAGACGTATCCGCTGAAGTGGTAGAAAAAGAATACCAGGTACAGCTGGata                >  2:1119239/1‑140 (MQ=255)                                                                                                                      ctgctAAAGGCACTGACGAAGAGCTGGTTAAACACATCGCTATGCACGTTGCTGCAAGCAAGCCAGAATTCATCAAACCGGAAGACGTATCCGCTGAAGTGGTAGAAAAAGAATACCAGGTACAGCTGGATATCGCGATg        <  1:44283/140‑1 (MQ=255)                                                                                                                       tgctAAAGGCACTGACGAAGAGCTGGTTAAACACATCGCTATGCACGTTGCTGCAAGTAAGCCAGAATTCATCAAACCGGAAGACGTATCCGCTGAAGTGGTAGAAAAAGAATACCAGGTACAGCTGGATATCGCGATGc       >  1:305012/1‑140 (MQ=255)                                                                                                                        gctAAAGGCACTGACGAAGAGCTGGTTAAACACATCGCTATGCACGTTGCTGCAAGCAAGCCAGAATTCATCAAACCGGAAGACGTATCCGCTGAAGTGGTAGAAAAAGAATACCAGGTACAGCTGGATATCGCGATGCa      <  2:1327349/140‑1 (MQ=255)                                                                                                                            aaGGCACTGACGAAGAGCTGGTTAAACACATCGCTATGCACGTTGCTGCAAGCAAGCCAGAATTCATCAAACCGGAAGACGTATCCGCTGAAGTGGTAGAAAAAGAATACCAGGTACAGCTGGATATCGCGATGCAGTCt  >  1:1177924/1‑140 (MQ=255)                                                                                                                                 |                                                                                                                                       AACGTGTTGCGCTGGTAGCGAAAATTGGTGAAAACATCAACATTCGCCGCGTTGCTGCGCTGGAAGGCGACGTTCTGGGTTCTTATCAGCACGGTGCGCGTATCGGCGTTCTGGTTGCTGCTAAAGGCGCTGACGAAGAGCTGGTTAAACACATCGCTATGCACGTTGCTGCAAGCAAGCCAGAATTCATCAAACCGGAAGACGTATCCGCTGAAGTGGTAGAAAAAGAATACCAGGTACAGCTGGATATCGCGATGCAGTCT  >  NC_000913/191209‑191471

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑