Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,444,382	A→G	T444T (ACA→ACG)	ydbH →	PF11739 family protein YdbH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,444,382	0	A	G	100.0%	153.1 / NA	44	T444T (ACA→ACG)	ydbH	PF11739 family protein YdbH
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (18/26);  total (18/26)

CGTTTGCAGGCCATCTTGCAGGCGCATGAAAATGAACTGGGCGATTTCGTGCTGCATATGGATGGGCTGGCGAATGATTTTCTCCCTGACGCTGGCCGCTGGCAGTGGCGCTACTGGGGAAAAGGGAGTTTTACACCGATGAATGCCACCTGGGATGTCGCAGGAAAAGGTGAGTGGCATGACAGCACGATTACGCTGACCGATCTCTCCACCGGTTTCGACCAGTTACAATACGGTACGATGACGGTAGAAAAGCCG  >  NC_000913/1444248‑1444505                                                                                                                                       |                                                                                                                            cgTTTGCAGGCCATCTTGCAGGCGCATGAAAATGAACTGGGCGATTTCGTGCTGCATATGGATGGGCTGGCGAATGATTTTCTCCCTGACGCTGGCCGCTGGCAGTGGCGCTACTGGGGAAAAGGGAGTTTTACGCCGAt                                                                                                                        >  1:1409374/1‑140 (MQ=255)     tGCAGGCCATCTTGCAGGCGCATGAAAATGAACTGGGCGATTTCGTGCTGCATATGGATGGGCTGGCGAATGATTTTCTCCCTGACGCTGGCCGCTGGCAGTGGCGCTACTGGGGAAAAGGGAGTTTTGCGCCGATGAAt                                                                                                                    <  1:568336/140‑1 (MQ=255)     tGCAGGCCATCTTGCAGGCGCATGAAAATGAACTGGGCGATTTCGTGCTGCATATGGATGGGCTGGCGAATGATTTTCTCCCTGACGCTGGCCGCTGGCAGTGGCGCTACTGGGGAAAAGGGAGTTTTACGCCGATGAAt                                                                                                                    <  2:849451/140‑1 (MQ=255)     tGCAGGCCATCTTGCAGGCGCATGAAAATAAACTGGGCGATTTCGTGCTGCATATGGATGGGCTGGCGAATGATTTTCTCCCTGACGCTGGCCGCTGGCAGTGGCGCTACTGGGGAAAAGGGAGTTTTACGCCGATGAAt                                                                                                                    <  2:440230/140‑1 (MQ=255)      gCAGGCCATCTTGCAGGCGCATGAAAATGAACTGGGCGATTTCGTGCTGCATATGGATGGGCTGGCGAATGATTTTCTCCCTGACGCTGGCCGCTGGCAGTGGCGCTACTGGGGAAAAGGGAGTTTTACGCCGATGAATg                                                                                                                   <  2:1409374/140‑1 (MQ=255)      gCAGGCCATCTTGCAGGCGCATGAAAATGAACTGGGCGATTTCGTGCTGCATATGGATGGGCTGGCGAATGATTTTCTCCCTGACGCTGGCCGCTGGCAGTGGCGCTACTGGGGAAAAGGGAGTTTTACGCCGATGAATg                                                                                                                   >  2:62034/1‑140 (MQ=255)       cAGGCCATCTTGCAGGCGCATGAAAATGAACTGGGCGATTTCGTGCTGCATATGGATGGGCTGGCGAATGATTTTCTCCCTGACGCTGGCCGCTGGCAGTGGCGCTACTGGGGAAAAGGGAGTTTTACGCCGATGAATGc                                                                                                                  <  2:45537/140‑1 (MQ=255)           ccATCTTGCAGGCGCATGAAAATGAACTGGGCGATTTCGTGCTGCATATGGATGGGCTGGCGAATGATTTTCTCCCTGACGCTGGCCGCTGGCAGTGGCGCTACTGGGGAAAAGGGAGTTTTACGCCGATGAATGCCAcc                                                                                                              <  1:351617/140‑1 (MQ=255)            cATCTTGCAGGCGCATGAAAATGAACTGGGCGATTTCGTGCTGCATATGGATGGGCTGGCGAATGATTTTCTCCCTGACGCTGGCCGCTGGCAGTGGCGCTACTGGGGAAAAGGGAGTTTTACGCCGATGAATGCCACCt                                                                                                             >  1:238576/1‑140 (MQ=255)                    aGGCGCATGAAAATGAACTGGGCGATTTCGTGCTGCATATGGATGGGCTGGCGAATGATTTTCTCCCTGACGCTGGCCGCTGGCAGTGGCGCTACTGGGGAAAAGGGAGTTTTACGCCGATGAATGCCACCTGGGATGTc                                                                                                     >  2:1371831/1‑140 (MQ=255)                     ggCGCATGAAAATGAACTGGGCGATTTCGTGCTGCATATGGATGGGCTGGCGAATGATTTTCTCCCTGACGCTGGCCGCTGGCAGTGGCGCTACTGGGGAAAAGGGAGTTTTACGCCGATGAATGCCACCTGGGATGTCg                                                                                                    <  2:238576/140‑1 (MQ=255)                        gcATGAAAATGAACTGGGCGATTTCGTGCTGCATATGGATGGGCTGGCGAATGATTTTCTCCCTGACGCTGGCCGCTGGCAGTGGCGCTACTGGGGAAAAGGGAGTTTTACGCCGATGAATGCCACCTGGGATGTCGCAg                                                                                                 >  1:280657/1‑140 (MQ=255)                                aTGAACTGGGCGATTTCGTGCTGCATATGGATGGGCTGGCGAATGATTTTCTCCCTGACGCTGGCCGCTGGCAGTGGCGCTACTGGGGAAAAGGGAGTTTTACGCCGATGAATGCCACCTGGGATGTCGCAGGAAAAGGt                                                                                         <  1:589545/140‑1 (MQ=255)                                        ggCGATTTCGTGCTGCATATGGATGGGCTGGCGAATGATTTTCTCCCTGACGCTGGCCGCTGGCAGTGGCGCTACTGGGGAAAAGGGAGTTTTACGCCGATGAATGCCACCTGGGATGTCGCAGGAAAAGGTGAg                                                                                      >  1:520902/1‑135 (MQ=255)                                        ggCGATTTCGTGCTGCATATGGATGGGCTGGCGAATGATTTTCTCCCTGACGCTGGCCGCTGGCAGTGGCGCTACTGGGGAAAAGGGAGTTTTACGCCGATGAATGCCACCTGGGATGTCGCAGGAAAAGGTGAg                                                                                      <  2:520902/135‑1 (MQ=255)                                           gATTTCGTGCTGCATATGGATGGGCTGGCGAATGATTTTCTCCCTGACGCTGGCCGCTGGCAGTGGCGCTACTGGGGAAAAGGGAGTTTTACGCCGATGAATGCCACCTGGGATGTCGCAGGAAAAGGTGAGTGGCATGa                                                                              <  2:519047/140‑1 (MQ=255)                                                cGTGCTGCATATGGATGGGCTGGCGAATGATTTTCTCCCTGACGCTGGCCGCTGGCAGTGGCGCTACTGGGGAAAAGGGAGTTTTACGCCGATGAATGCCACCTGGGATGTCGCAGGAAAAGGTGAGTGGCATGACAGCa                                                                         <  1:417728/140‑1 (MQ=255)                                                  tgctgcATATGGATGGGCTGGCGAATGATTTTCTCCCTGACGCTGGCCGCTGGCAGTGGCGCTACTGGGGAAAAGGGAGTTTTACGCCGATGAATGCCACCTGGGATGTCGCAGGAAAAGGTGAGTGGCATGACAGCACg                                                                       >  2:878120/1‑140 (MQ=255)                                                       cATATGGATGGGCTGGCGAATGATTTTCTCCCTGACGCTGGCCGCTGGCAGTGGCGCTACTGGGGAAAAGGGAGTTTTACGCCGATGAATGCCACCTGGGATGTCGCAGGAAAAGGTGAGTGGCATGACAGCACGATTAc                                                                  >  1:1152090/1‑140 (MQ=255)                                                              atggGCTGGCGAATGATTTTCTCCCTGACGCTGGCCGCTGGCAGTGGCGCTACTGGGGAAAAGGGAGTTTTACGCCGATGAATGCCACCTGGGATGTCGCAGGAAAAGGTGAGTGGCATGACAGCACGATTACGCTGAcc                                                           <  2:1032979/140‑1 (MQ=255)                                                                 ggCTGGCGAATGATTTTCTCCCTGACGCTGGCCGCTGGCAGTGGCGCTACTGGGGAAAAGGGAGTTTTACGCCGATGAATGCCACCTGGGATGTCGCAGGAAAAGGTGAGTGGCATGACAGCACGATTACGCTGACCGAt                                                        >  2:1272481/1‑140 (MQ=255)                                                                 ggCTGGCGAATGATTTTCTCCCTGACGCTGGCCGCTGGCAGTGGCGCTACTGGGGAAAAGGGAGTTTTACGCCGATGAATGCCACCTGGGATGTCGCAGGAAAAGGTGAGTGGCATGACAGCACGATTACGCTGACCGAt                                                        >  2:1382717/1‑140 (MQ=255)                                                                 ggCTGGCGAATGATTTTCTCCCTGACGCTGGCCGCTGGCAGTGGCGCTACTGGGGAAAAGGGAGTTTTACGCCGATGAATGCCACCTGGGATGTCGCAGGAAAAGGTGAGTGGCATGACAGCACGATTACGCTGACCGAt                                                        <  1:1272481/140‑1 (MQ=255)                                                                    tGGCGAATGATTTTCTCCCTGACGCTGGCCGCTGGCAGTGGCGCTACTGGGGAAAAGGGAGTTTTACGCCGATGAATGCCACCTGGGATGTCGCAGGAAAAGGTGAGTGGCATGACAGCACGATTACGCTGACCGAtctc                                                     >  2:905385/1‑140 (MQ=255)                                                                      gCGAATGATTTTCTCCCTGACGCTGGCCGCTGGCAGTGGCGCTACTGGGGAAAAGGGAGTTTTACGCCGATGAATGCCACCTGGGATGTCGCAGGAAAAGGTGAGTGGCATGACAGCACGATTACGCTGACCGAtctctc                                                   >  1:776717/1‑140 (MQ=255)                                                                       cGAATGATTTTCTCCCTGACGCTGGCCGCTGGCAGTGGCGCTACTGGGGAAAAGGGAGTTTTACGCCGATGAATGCCACCTGGGATGTCGCAGGAAAAGGTGAGTGGCATGACAGCACGATTACGCTGACCGATCTCTcc                                                  >  2:1230025/1‑140 (MQ=255)                                                                               tttCTCCCTGACGCTGGCCGCTGGCAGTGGCGCTACTGGGGAAAAGGGAGTTTTACGCCGATGAATGCCACCTGGGATGTCGCAGGAAAAGGTGAGTGGCATGACAGCACGATTACGCTGACCGATCTCTCCACCGGttt                                          <  2:102220/140‑1 (MQ=255)                                                                                   tcCCTGACGCTGGCCGCTGGCAGTGGCGCTACTGGGGAAAAGGGAGTTTTACGCCGATGAATGCCACCTGGGATGTCGCAGGAAAAGGTGAGTGGCATGACAGCACGATTACGCTGACCGATCTCTCCACCGGTTTCGAc                                      <  1:1382717/140‑1 (MQ=255)                                                                                   tcCCTGACGCTGGCCGCTGGCAGTGGCGCTACTGGGGAAAAGGGAGTTTTACGCCGATGAATGCCACCTGGGATGTCGCAGGAAAAGGTGAGTGGCATGACAGCACGATTACGCTGACCGATCTCTCCACCGGTTTCGAc                                      <  1:1380677/140‑1 (MQ=255)                                                                                    cccTGACGCTGGCCGCTGGCAGTGGCGCTACTGGGGAAAAGGGAGTTTTACGCCGATGAATGCCACCTGGGATGTCGCAGGAAAAGGTGAGTGGCATGACAGCACGATTACGCTGACCGATCTCTCCACCGGTTTCGAcc                                     <  1:878120/140‑1 (MQ=255)                                                                                    cccTGACGCTGGCCGCTGGCAGTGGCGCTACTGGGGAAAAGGGAGTTTTACGCCGATGAATGCCACCTGGGATGTCGCAGGAAAAGGTGAGTGGCATGACAGCACGATTACGCTGACCGATCTCTCCACCGGTTTCGAcc                                     <  2:350641/140‑1 (MQ=255)                                                                                      cTGACGCTGGCCGCTGGCAGTGGCGCTACTGGGGAAAAGGGAGTTTTACGCCGATGAATGCCACCTGGGATGTCGCAGGAAAAGGTGAGTGGCATGACAGCACGATTACGCTGACCGATCTCTCCACCGGTTTCGACCAg                                   <  2:280657/140‑1 (MQ=255)                                                                                              ggccgctggcAGTGGCGCTACTGGGGAAAAGGGAGTTTTACGCCGATGAATGCCACCTGGGATGTCGCAGGAAAAGGTGAGTGGCATGACAGCACGATTACGCTGACCGATCTCTCCACCGGTTTCGACCAGTTACAATa                           <  1:635602/140‑1 (MQ=255)                                                                                              ggccgctggcAGTGGCGCTACTGGGGAAAAGGGAGTTTTACGCCGATGAATGCCACCTGGGATGTCGCAGGAAAAGGTGAGTGGCATGACAGCACGATTACGCTGACCGATCTCTCCACCGGTTTCGACCAGTTACAATa                           <  2:185436/140‑1 (MQ=255)                                                                                              ggccgctggcAGTGGCGCTACTGGGGAAAAGGGAGTTTTACGCCGATGAATGCCACCTGGGATGTCGCAGGAAAAGGTGAGTGGCATGACAGCACGATTACGCTGACCGATCTCTCCACCGGTTTCGACCAGTTACAATa                           <  1:1230025/140‑1 (MQ=255)                                                                                              ggccgctggcAGTGGCGCTACTGGGGAAAAGGGAGTTTTACGCCGATGAATGCCACCTGGGATGTCGCAGGAAAAGGTGAGTGGCATGACAGCACGATTACGCTGACCGATCTCTCCACCGGTTTCGACCAGTTACAATa                           <  2:862602/140‑1 (MQ=255)                                                                                                      gcAGTGGCGCTACTGGGGAAAAGGGAGTTTTACGCCGATGAATGCCACCTGGGATGTCGCAGGAAAAGGTGAGTGGCATGACAGCACGATTACGCTGACCGATCTCTCCACCGGTTTCGACCAGTTACAATACGGTACGa                   <  2:1152090/140‑1 (MQ=255)                                                                                                                tACTGGGGAAAAGGGAGTTTTACGCCGATGAATGCCACCTGGGATGTCGCAGGAAAAGGTGAGTGGCATGACAGCACGATTACGCTGACCGATCTCTCCACCGGTTTCGACCAGTTACAATa                           >  2:289175/1‑122 (MQ=255)                                                                                                                tACTGGGGAAAAGGGAGTTTTACGCCGATGAATGCCACCTGGGATGTCGCAGGAAAAGGTGAGTGGCATGACAGCACGATTACGCTGACCGATCTCTCCACCGGTTTCGACCAGTTACAATa                           <  1:289175/122‑1 (MQ=255)                                                                                                                tACTGGGGAAAAGGGAGTTTTACGCCGATGAATGCCACCTGGGATGTCGCAGGAAAAGGTGAGTGGCATGACAGCACGATTACGCTGACCGATCTCTCCACCGGTTTCGACCAGTTACAATACGGTACGATGACGGTAGa         >  1:1055642/1‑140 (MQ=255)                                                                                                                 aCTGGGGAAAAGGGAGTTTTACGCCGATGAATGCCACCTGGGATGTCGCAGGAAAAGGTGAGTGGCATGACAGCACGATTACGCTGACCGATCTCTCCACCGGTTTCGACCAGTTACAATACGGTACGATGACGGTAGaa        >  2:856239/1‑140 (MQ=255)                                                                                                                     gggAAAAGGGAGTTTTACGCCGATGAATGCCACCTGGGATGTCGCAGGAAAAGGTGAGTGGCATGACAGCACGATTACGCTGACCGATCTCTCCACCGGTTTCGACCAGTTACAATACGGTACGATGACGGTAGAAAAGc    >  2:642098/1‑140 (MQ=255)                                                                                                                     gggAAAAGGGAGTTTTACGCCGATGAATGCCACCTGGGATGTCGCAGGAAAAGGTGAGTGGCATGACAGCACGATTACGCTGACCGATCTCTCCACCGGTTTCGACCAGTTACAATACGGTACGATGACGGTAGAAAAGc    >  2:578358/1‑140 (MQ=255)                                                                                                                       gAAAAGGGAGTTTTACGCCGATGAATGCCACCTGGGATGTCGCAGGAAAAGGTGAGTGGCATGACAGCACGATTACGCTGACCGATCTCTCCACCGGTTTCGACCAGTTACAATACGGTACGATGACGGTAGAAAAGCcg  <  2:776717/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                            CGTTTGCAGGCCATCTTGCAGGCGCATGAAAATGAACTGGGCGATTTCGTGCTGCATATGGATGGGCTGGCGAATGATTTTCTCCCTGACGCTGGCCGCTGGCAGTGGCGCTACTGGGGAAAAGGGAGTTTTACACCGATGAATGCCACCTGGGATGTCGCAGGAAAAGGTGAGTGGCATGACAGCACGATTACGCTGACCGATCTCTCCACCGGTTTCGACCAGTTACAATACGGTACGATGACGGTAGAAAAGCCG  >  NC_000913/1444248‑1444505

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑