Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,456,917	A→G	D163G (GAC→GGC)	paaE →	phenylacetyl‑CoA 1,2‑epoxidase, reductase subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,456,917	0	A	G	100.0%	203.0 / NA	58	D163G (GAC→GGC)	paaE	phenylacetyl‑CoA 1,2‑epoxidase, reductase subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (27/31);  total (27/31)

TTGCAGCAGGATCAGGTATTACGCCAATGCTGGCGATTATCGCCACCACTTTACAAACCGAGCCTGAAAGTCAGTTCACCCTGATCTACGGTAACCGTACCAGCCAGAGCATGATGTTTCGCCAGGCACTGGCAGACCTGAAAGACAAATATCCTCAGCGTTTACAGTTGTTGTGCATTTTCAGTCAGGAAACCCTCGACAGCGATCTGCTTCACGGGCGTATTGACGGTGAAAAATTACAGTCACTTGGGGCCTCGCTCATTAATTTT  >  NC_000913/1456782‑1457050                                                                                                                                        |                                                                                                                                      ttGCAGCAGGATCAGGTATTACGCCAATGCTGGCGATTATCGCCACCACTTTACAAACCGAGCCTGAAAGTCAGTTCACCCTGATCTACGGTAACCGTACCAGCCAGAGCATGATGTTTCGCCAGGCACTGGCAGGCCTg                                                                                                                                   <  1:494538/140‑1 (MQ=255)  tGCAGCAGGATCAGGTATTACGCCAATGCTGGCGATTATCGCCACCACTTTACAAACCGAGCCTGAAAGTCAGTTCACCCTGATCTACGGTAACCGTACCAGCCAGAGCATGATGTTTCGCCAGGCACTGGCAGGCCTGa                                                                                                                                  <  1:762453/140‑1 (MQ=255)    cagcagGATCAGGTATTACGCCAATGCTGGCGATTATCGCCACCACTTTACAAACCGAGCCTGAAAGTCAGTTCACCCTGATCTACGGTAACCGTACCAGCCAGAGCATGATGTTTCGCCAGGCACTGGCAGGCCTGaaa                                                                                                                                <  1:1022597/140‑1 (MQ=255)    cagcagGATCAGGTATTACGCCAATGCTGGCGATTATCGCCACCACTTTACAAACCGAGCCTGAAAGTCAGTTCACCCTGATCTACGGTAACCGTACCAGCCAGAGCATGATGTTTCGCCAGGCACTGGCAGGCCTGaaa                                                                                                                                <  1:1292770/140‑1 (MQ=255)    cagcagGATCAGGTATTACGCCAATGCTGGCGATTATCGCCACCACTTTACAAACCGAGCCTGAAAGTCAGTTCACCCTGATCTACGGTAACCGTACCAGCCAGAGCATGATGTTTCGCCAGGCACTGGCAGGCCTGaaa                                                                                                                                <  1:611486/140‑1 (MQ=255)    cagcagGATCAGGTATTACGCCAATGCTGGCGATTATCGCCACCACTTTACAAACCGAGCCTGAAAGTCAGTTCACCCTGATCTACGGTAACCGTACCAGCCAGAGCATGATGTTTCGCCAGGCACTGGCAGGCCTGaaa                                                                                                                                <  1:278122/140‑1 (MQ=255)         ggATCAGGTATTACGCCAATGCTGGCGATTATCGCCACCACTTTACAAACCGAGCCTGAAAGTCAGTTCACCCTGATCTACGGTAACCGTACCAGCCAGAGCATGATGTTTCGCCAGGCACTGGCAGGCCTGAAAGACaa                                                                                                                           >  2:78732/1‑140 (MQ=255)            tCAGGTATTACGCCAATGCTGGCGATTATCGCCACCACTTTACAAACCGAGCCTGAAAGTCAGTTCACCCTGATCTACGGTAACCGTACCAGCCAGAGCATGATGTTTCGCCAGGCACTGGCAGGCCTGaaa                                                                                                                                <  2:755456/132‑1 (MQ=255)            tCAGGTATTACGCCAATGCTGGCGATTATCGCCACCACTTTACAAACCGAGCCTGAAAGTCAGTTCACCCTGATCTACGGTAACCGTACCAGCCAGAGCATGATGTTTCGCCAGGCACTGGCAGGCCTGaaa                                                                                                                                >  1:755456/1‑132 (MQ=255)                  aTTACGCCAATGCTGGCGATTATCGCCACCACTTTACAAACCGAGCCTGAAAGTCAGTTCACCCTGATCTACGGTAACCGTACCAGCCAGAGCATGATGTTTCGCCAGGCACTGGCAGGCCTGAAAGACAAATATCCTCa                                                                                                                  <  1:78732/140‑1 (MQ=255)                    tACGCCAATGCTGGCGATTATCGCCACCACTTTACAAACCGAGCCTGAAAGTCAGTTCACCCTGATCTACGGTAACCGTACCAGCCAGAGCATGATGTTTCGCCAGGCACTGGCAGGCCTGAAAGACAAATATCCTc                                                                                                                   >  2:994875/1‑137 (MQ=255)                    tACGCCAATGCTGGCGATTATCGCCACCACTTTACAAACCGAGCCTGAAAGTCAGTTCACCCTGATCTACGGTAACCGTACCAGCCAGAGCATGATGTTTCGCCAGGCACTGGCAGGCCTGAAAGACAAATATCCTc                                                                                                                   <  1:994875/137‑1 (MQ=255)                    tACGCCAATGCTGGCGATTATCGCCACCACTTTACAAACCGAGCCTGAAAGTCAGTTCACCCTGATCTACGGTAACCGTACCAGCCAGAGCATGATGTTTCGCCAGGCACTGGCAGGCCTGAAAGACAAATATCCTCAGc                                                                                                                >  2:685938/1‑140 (MQ=255)                     aCGCCAATGCTGGCGATTATCGCCACCACTTTACAAACCGAGCCTGAAAGTCAGTTCACCCTGATCTACGGTAACCGTACCAGCCAGAGCATGATGTTTCGCCAGGCACTGGCAGGCCTGAAAGACAAATATCCTCAGCg                                                                                                               >  2:527054/1‑140 (MQ=255)                     aCGCCAATGCTGGCGATTATCGCCACCACTTTACAAACCGAGCCTGAAAGTCAGTTCACCCTGATCTACGGTAACCGTACCAGCCAGAGCATGATGTTTCGCCAGGCACTGGCAGGCCTGAAAGACAAATATCCTCAGCg                                                                                                               >  1:780351/1‑140 (MQ=255)                      cGCCAATGCTGGCGATTATCGCCACCACTTTACAAACCGAGCCTGAAAGTCAGTTCACCCTGATCTACGGTAACCGTACCAGCCAGAGCATGATGTTTCGCCAGGCACTGGCAGGCCTGAAAGACAAATATCCTCAGCGt                                                                                                              >  2:819113/1‑140 (MQ=255)                      cGCCAATGCTGGCGATTATCGCCACCACTTTACAAACCGAGCCTGAAAGTCAGTTCACCCTGATCTACGGTAACCGTACCAGCCAGAGCATGATGTTTCGCCAGGCACTGGCAGGCCTGAAAGACAAATATCCTCAGCGt                                                                                                              >  1:975313/1‑140 (MQ=255)                      cGCCAATGCTGGCGATTATCGCCACCACTTTACAAACCGAGCCTGAAAGTCAGTTCACCCTGATCTACGGTAACCGTACCAGCCAGAGCATGATGTTTCGCCAGGCACTGGC‑GGCCTGAAAGACAAATATCCTCAGCGtt                                                                                                             >  2:1003394/1‑140 (MQ=255)                          aaTGCTGGCGATTATCGCCACCACTTTACAAACCGAGCCTGAAAGTCAGTTCACCCTGATCTACGGTAACCGTACCAGCCAGAGCATGATGTTTCGCCAGGCACTGGCAGGCCTGAAAGACAAATATCCTCAGCGTTTAc                                                                                                          <  1:1405112/140‑1 (MQ=255)                           aTGCTGGCGATTATCGCCACCACTTTACAAACCGAGCCTGAAAGTCAGTTCACCCTGATCTACGGTAACCGTACCAGCCAGAGCATGATGTTTCGCCAGGCACTGGCAGGCCTGAAAGACAAATATCCTCAGCGTTTACa                                                                                                         >  2:919153/1‑140 (MQ=255)                           aTGCTGGCGATTATCGCCACCACTTTACAAACCGAGCCTGAAAGTCAGTTCACCCTGATCTACGGTAACCGTACCAGCCAGAGCATGATGTTTCGCCAGGCACTGGCAGGCCTGAAAGACAAATATCCTCAGCGTTTACa                                                                                                         >  1:641524/1‑140 (MQ=255)                           aTGCTGGCGATTATCGCCACCACTTTACAAACCGAGCCTGAAAGTCAGTTCACCCTGATCTACGGTAACCGTACCAGCCAGAGCATGATGTTTCGCCAGGCACTGGCAGGCCTGAAAGACAAATATCCTCAGCGTTTACa                                                                                                         >  1:1022718/1‑140 (MQ=255)                            tGCTGGCGATTATCGCCACCACTTTACAAACCGAGCCTGAAAGTCAGTTCACCCTGATCTACGGTAACCGTACCAGCCAGTGCATGATGTTTCGCCAGGCACTGGCAGGCCTGAAAGACAAATATCCTCAGCGTTTACAg                                                                                                        <  2:975313/140‑1 (MQ=255)                            tGCTGGCGATTATCGCCACCACTTTACAAACCGAGCCTGAAAGTCAGTTCACCCTGATCTACGGTAACCGTACCAGCCAGAGCATGATGTTTCGCCAGGCACTGGCAGGCCTGAAAGACAAATATCCTCAGc                                                                                                                >  2:1320328/1‑132 (MQ=255)                            tGCTGGCGATTATCGCCACCACTTTACAAACCGAGCCTGAAAGTCAGTTCACCCTGATCTACGGTAACCGTACCAGCCAGAGCATGATGTTTCGCCAGGCACTGGCAGGCCTGAAAGACAAATATCCTCAGc                                                                                                                <  1:1320328/132‑1 (MQ=255)                            tGCTGGCGATTATCGCCACCACTTTACAAACCGAGCCTGAAAGTCAGTTCACCCTGATCTACGGTAACCGTACCAGCCAGAGCATGATGTTTCGCCAGGCACTGGCAGGCCTGAAAGACAAATATCCTCAGCGTTTACAg                                                                                                        <  2:1219124/140‑1 (MQ=255)                            tGCTGGCGATTATCGCCACCACTTTACAAACCGAGCCTGAAAGTCAGTTCACCCTGATCTACGGTAACCGTACCAGCCAGAGCATGATGTTTCGCCAGGCACTGGCAGGCCTGAAAGACAAATATCCTCAGCGTTTACAg                                                                                                        <  2:1186515/140‑1 (MQ=255)                                 gCGATTATCGCCACCACTTTACAAACCGAGCCTGAAAGTCAGTTCACCCTGATCTACGGTAACCGTACCAGCCAGAGCATGATGTTTCGCCAGGCACTGGCAGGCCTGAAAGACAAATATCCTCAGCGTTTACAgttgtt                                                                                                   >  2:509561/1‑140 (MQ=255)                                      tATCGCCACCACTTTACAAACCGAGCCTGAAAGTCAGTTCACCCTGATCTACGGTAACCGTACCAGCCAGAGCATGATGTTTCGCCAGGCACTGGCAGGCCTGAAAGACAAATATCCTCAGCGTTTACAGTTGTTGTGCa                                                                                              >  2:718877/1‑140 (MQ=255)                                         cGCCACCACTTTACAAACCGAGCCTGAAAGTCAGTTCACCCTGATCTACGGTAACCGTACCAGCCAGAGCATGATGTTTCGCCAGGCACTGGC‑GGCCTGAAAGACAAATATCCTCAGCGTTTACAGTTGTTGTGCAtttt                                                                                          <  1:1003394/140‑1 (MQ=255)                                            caccacTTTACAAACCGAGCCTGAAAGTCAGTTCACCCTGATCTACGGTAACCGTACCAGCCAGAGCATGATGTTTCGCCAGGCACTGGCAGGCCTGAAAGACAAATATCCTCAGCGTTTACAGTTGTTGTGCATTTtca                                                                                        <  1:926027/140‑1 (MQ=255)                                                 cTTTACAAACCGAGCCTGAAAGTCAGTTCACCCTGATCTACGGTAACCGTACCAGCCAGAGCATGATGTTTCGCCAGGCACTGGCAGGCCTGAAAGACAAATATCCTCAGCGTTTACAGTTGTTGTGCATTTtcagtcag                                                                                   >  1:1385877/1‑140 (MQ=255)                                                  tttACAAACCGAGCCTGAAAGTCAGTTCACCCTGATCTACGGTAACCGTACCAGCCAGAGCATGATGTTTCGCCAGGCACTGGCAGGCCTGAAAGACAAATATCCTCAGCGTTTACAGTTGTTGTGCATTTTCAGTCAgg                                                                                  <  1:718877/140‑1 (MQ=255)                                                           cGAGCCTGAAAGTCAGTTCACCCTGATCTACGGTAACCGTACCAGCCAGAGCATGATGTTTCGCCAGGCACTGGCAGGCCTGAAAGACAAATATCCTCAGCGTTTACAGTTGTTGTGCATTTTCAGTCAGGAAACCCTCg                                                                         >  1:261598/1‑140 (MQ=255)                                                            gAGCCTGAAAGTCAGTTCACCCTGATCTACGGTAACCGTACCAGCCAGAGCATGATGTTTCGCCAGGCACTGGCAGGCCTGAAAGACAAATATCCTCAGCGTTTACAGTTGTTGTGCATTTTCAGTCAGGAAACCCTCGa                                                                        <  2:428886/140‑1 (MQ=255)                                                            gAGCCTGAAAGTCAGTTCACCCTGATCTACGGTAACCGTACCAGCCAGAGCATGATGTTTCGCCAGGCACTGGCAGGCCTGAAAGACAAATATCCTCAGCGTTTACAGTTGTTGTACATTTTCAGTCAGGAAACCCTCGa                                                                        >  2:427531/1‑140 (MQ=255)                                                              gCCTGAAAGTCAGTTCACCCTGATCTACGGTAACCGTACCAGCCAGAGCATGATGTTTCGCCAGGCACTGGCAGGCCTGAAAGACAAATATCCTCAGCGTTTACAGTTGTTGTGCATTTTCAGTCAGGAAACCCTCGACa                                                                      >  1:1383130/1‑140 (MQ=255)                                                                  gAAAGTCAGTTCACCCTGATCTACGGTAACCGTACCAGCCAGAGCATGATGTTTCGCCAGGCACTGGCAGGCCTGAAAGACAAATATCCTCAGCGTTTACAGTTGTTGTGCATTTTCAGTCAGGAAACCCTCGACAGCGa                                                                  <  2:118004/140‑1 (MQ=255)                                                                        cAGTTCACCCTGATCTACGGTAACCGTACCAGCCAGAGCATGATGTTTCGCCAGGCACTGGCAGGCCTGAAAGACAAATATCCTCAGCGTTTACAGTTGTTGTACATTTTCAGTCAGGAAACCCTCGACAGCGATCTGCt                                                            <  1:427531/140‑1 (MQ=255)                                                                           ttCACCCTGATCTACGGTAACCGTACCAGCCAGAGCATGATGTTTCGCCAGGCACTGGCAGGCCTGAAAGACAAATATCCTCAGCGTTTACAGTTGTTGTGCATTTTCAGTCAGGAAACCCTCGACAGCGATCTGCTTCa                                                         <  1:819113/140‑1 (MQ=255)                                                                                ccTGATCTACGGTAACCGTACCAGCCAGAGCATGATGTTTCGCCAGGCACTGGCAGGCCTGAAAGACAAATATCCTCAGCGTTTACAGTTGTTGTGCATTTTCAGTCAGGAAACCCTCGACAGCGATCTGCTTCACGGGc                                                    >  2:1101533/1‑140 (MQ=255)                                                                                     tCTACGGCAACCGTACCAGCCAGAGCATGATGTCTCGCCAGGCACTGGCAGGCCTGAAAGACAAATATCCTCAACGTTTAGAGATATTGGGCATTTTCCGTCAGGCAACCCTCGCCAGCGATCTGCTTGACGGGCGTAtt                                               >  2:896091/1‑140 (MQ=255)                                                                                       tACGGTAACCGTACCAGCCAGAGCATGATGTTTCGCCAGGCACTGGCAGGCCTGAAAGACAAATATCCTCAGCGTTTACAGTTGTTGTGCATTTTCAGTCAGGAAACCCTCGACAGCGATCTGCTTCACGGGCGTATTGa                                             <  2:1022718/140‑1 (MQ=255)                                                                                            tAACCGTACCAGCCAGAGCATGATGTTTCGCCAGGCACTGGCAGGCCTGAAAGACAAATATCCTCAGCGTTTACAGTTGTTGTGCATTTTCAGTCAGGAAACCCTCGACAGCGATCTGCTTCACGGGCGTATTGACGGTg                                        >  2:7040/1‑140 (MQ=255)                                                                                              aCCGTACCAGCCAGAGCATGATGTTTCGCCAGGCACTGGCAGGCCTGAAAGACAAATATCCTCAGCGTTTACAGTTGTTGTGCATTTTCAGTCAGGAAACCCTCGACAGCGATCTGCTTCACGGGCGTATTGACGGTGaa                                      >  1:131543/1‑140 (MQ=255)                                                                                               ccGTACCAGCCAGAGCATGATGTTTCGCCAGGCACTGGCAGGCCTGAAAGACAAATATCCTCAGCGTTTACAGTTGTTGTGCATTTTCAGTCAGGAAACCCTCGACAGCGATCTGCTTCACGGGCGTATTGACGGTGaaa                                     >  1:485940/1‑140 (MQ=255)                                                                                                   aCCAGCCAGAGCATGATGTTTCGCCAGGCACTGGCAGGCCTGAAAGACAAATATCCTCAGCGTTTACAGTTGTTGTGCATTTTCAGTCAGGAAACCCTCGACAGCGATCTGCTTCACGGGCGTATTGACGGTGAAAAAtt                                 >  2:550128/1‑140 (MQ=255)                                                                                                      agccagAGCATGATGTTTCGCCAGGCACTGGCAGGCCTGAAAGACAAATATCCTCAGCGTTTACAGTTGTTGTGCATTTTCAGTCAGGAAACCCTCGACAGCGATCTGCTTCACGGGCGTATTGACGGTGAAAAATTACa                              <  2:641524/140‑1 (MQ=255)                                                                                                      agccagAGCATGATGTTTCGCCAGGCACTGGCAGGCCTGAAAGACAAATATCCTCAGCGTTTACAGTTGTTGTGCATTTTCAGTCAGGAAACCCTCGACAGCGATCTGCTTCACGGGCGTATTGACGGTGAAAAATTACa                              <  1:1297172/140‑1 (MQ=255)                                                                                                      agccagAGCATGATGTTTCGCCAGGCACTGGCAGGCCTGAAAGACAAATATCCTCAGCGTTTACAGTTGTTGTGCATTTTCAGTCAGGAAACCCTCGACAGCGATCTGCTTCACGGGCGTATTGACGGTGAAAAATTACa                              <  1:685938/140‑1 (MQ=255)                                                                                                      agccagAGCATGATGTTTCGCCAGGCACTGGCAGGCCTGAAAGACAAATATCCTCAGCGTTTACAGTTGTTGTGCATTTTCAGTCAGGAAACCCTCGACAGCGATCTGCTTCACGGGCGTATTGACGGTGAAAAATTACa                              <  2:1385877/140‑1 (MQ=255)                                                                                                      agccagAGCATGATGTTTCGCCAGGCACTGGCAGGCCTGAAAGACAAATATCCTCAGCGTTTACAGTTGTTGTGCATTTTCAGTCAGGAAACCCTCGACAGCGATCTGCTTCACGGGCGTATTGACGGTGAAAAATTACa                              >  2:1297172/1‑140 (MQ=255)                                                                                                       gccagAGCATGATGTTTCGCCAGGCACTGGCAGGCCTGAAAGACAAATATCCTCAGCGTTTACAGTTGTTGTGCATTTTCAGTCAGGAAACCCTCGACAGCGATCTGCTTCACGGGCGTATTGACGGTGAAAAATTACAg                             <  2:485940/140‑1 (MQ=255)                                                                                                       gccagAGCATGATGTTTCGCCAGGCACTGGCAGGCCTGAAAGACAAATATCCTCAGCGTTTACAGTTGTTGTGCATTTTCAGTCAGGAAACCCTCGACAGCGATCTGCTTCACGGGCGTATTGACGGTGAAAAATTACAg                             <  1:509561/140‑1 (MQ=255)                                                                                                        ccagAGCATGATGTTTCGCCAGGCACTGGCAGGCCTGAAAGACAAATATCCTCAGCGTTTACAGTTGTTGTGCATTTTCAGTCAGGAAACCCTCGACAGCGATCTGCTTCACGGGCGTATTGACGGTGAAAAATTACAGt                            >  1:752460/1‑140 (MQ=255)                                                                                                            agCATGATGTTTCGCCAGGCACTGGCAGGCCTGAAAGACAAATATCCTCAGCGTTTACAGTTGTTGTGCATTTTCAGTCAGGAAACCCTCGACAGCGATCTGCTTCACGGGCGTATTGACGGTGAAAAATTACAGTCACt                        >  1:1337540/1‑140 (MQ=255)                                                                                                              cATGATGTTTCGCCAGGCACTGGCAGGCCTGAAAGACAAATATCCTCAGCGTTTACAGTTGTTGTGCATTTTCAGTCAGGAAACCCTCGACAGCGATCTGCTTCACGGGCGTATTGACGGTGAAAAATTACAGTCACTTg                      <  2:131543/140‑1 (MQ=255)                                                                                                                tgatgTTTCGCCAGGCACTGGCAGGCCTGAAATACAAATATCCTCAGCGTTTACAGTTGTTGTGCATTTTCAGTCAGGAAACCCTCGACAGCGATCTGCTTCACGGGCGTATTGACGGTGAAAAATTACAGTCACTTggg                    <  1:367211/140‑1 (MQ=255)                                                                                                                                  tGGCAGGCCTGAAAGACAAATATCCTCAGCGTTTACAGTTGTTGTGCATTTTCAGTCAGGAAACCCTCGACAGCGATCTGCTTCACGGGCGTATTGACGGTGAAAAATTACAGTCACTTGGGGCCTCGCTCATTAAtttt  <  1:7040/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                      TTGCAGCAGGATCAGGTATTACGCCAATGCTGGCGATTATCGCCACCACTTTACAAACCGAGCCTGAAAGTCAGTTCACCCTGATCTACGGTAACCGTACCAGCCAGAGCATGATGTTTCGCCAGGCACTGGCAGACCTGAAAGACAAATATCCTCAGCGTTTACAGTTGTTGTGCATTTTCAGTCAGGAAACCCTCGACAGCGATCTGCTTCACGGGCGTATTGACGGTGAAAAATTACAGTCACTTGGGGCCTCGCTCATTAATTTT  >  NC_000913/1456782‑1457050

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑