Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	200,900	A→G	N140S (AAC→AGC)	skp →	periplasmic chaperone Skp

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	200,900	0	A	G	100.0%	185.2 / NA	54	N140S (AAC→AGC)	skp	periplasmic chaperone Skp
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (21/33);  total (21/33)

GCTCAGAAAGCGCAGGCTTTTGAGCAGGATCGCGCACGTCGTTCCAACGAAGAACGCGGCAAACTGGTTACTCGTATCCAGACTGCTGTGAAATCCGTTGCCAACAGCCAGGATATCGATCTGGTTGTTGATGCAAACGCCGTTGCTTACAACAGCAGCGATGTAAAAGACATCACTGCCGACGTACTGAAACAGGTTAAATAAGTAATGCCTTCAATTCGACTGGCTGATTTAGCGCAGCAGTTGGATGCAGAACTACACGGTGATGGCGAT  >  NC_000913/200764‑201036                                                                                                                                         |                                                                                                                                         gCTCAGAAAGCGCAGGCTTTTGAGCAGGATCGCGCACGTCGTTCCAACGAAGAACGCGGCAAACTGGTTACTCGTATCCAGACTGCTGTGAAATCCGTTGCCAACAGCCAGGATATCGATCTGGTTGTTGATGCAAgcgc                                                                                                                                       <  2:89898/140‑1 (MQ=255)   tCAGAAAGCGCAGGCTTTTGAGCAGGATCGCGCACGTCGTTCCAACGAAGAACGCGGCAAACTGGTTACTCGTATCCAGACTGCTGTGAAATCCGTTGCCAACAGCCAGGATATCGATCTGGTTGTTGATGCAAGCGCCg                                                                                                                                     <  1:672596/140‑1 (MQ=255)   tCAGAAAGCGCAGGCTTTTGAGCAGGATCGCGCACGTCGTTCCAACGAAGAACGCGGCAAACTGGTTACTCGTATCCAGACTGCTGTGAAATCCGTTGCCAACAGCCAGGATATCGATCTGGTTGTTGATGCAAGCGCCg                                                                                                                                     <  2:1089413/140‑1 (MQ=255)        aaGCGCAGGCTTTTGAGCAGGATCGCGCACGTCGTTCCAACGAAGAACGCGGCAAACTGGTTACTCGTATCCAGACTGCTGTGAAATCCGTTGCCAACAGCCAGGATATCGATCTGGTTGTTGATGCAAGCGCCGTTGCt                                                                                                                                <  1:985373/140‑1 (MQ=255)           cgcAGGCTTTTGAGCAGGATCGCGCACGTCGTTCCAACGAAGAACGCGGCAAACTGGTTACTCGTATCCAGACTGCTGTGAAATCCGTTGCCAACAGCCAGGATATCGATCTGGTTGTTGATGCAAGCGCCGTTGCTTac                                                                                                                             <  1:318494/140‑1 (MQ=255)                   tttGAGCAGGATCGCGCACGTCGTTCCAACGAAGAACGCGGCAAACTGGTTACTCGTATCCAGACTGCTGTGAAATCCGTTGCCAACAGCCAGGATATCGATCTGGTTGTTGATGCAAGCGCCGTTGCTTACAAcagcag                                                                                                                     <  2:157658/140‑1 (MQ=255)                   tttGAGCAGGATCGCGCACGTCGTTCCAACGAAGAACGCGGCAAACTGGTTACTCGTATCCAGACTGCTGTGAAATCCGTTGCCAACAGCCAGGATATCGATCTGGTTGTTGATGCAAGCGCCGTTGCTTACAAcagcag                                                                                                                     <  1:734289/140‑1 (MQ=255)                   tttGAGCAGGATCGCGCACGTCGTTCCAACGAAGAACGCGGCAAACTGGTTACTCGTATCCAGACTGCTGTGAAATCCGTTGCCAACAGCCAGGATATCGATCTGGTTGTTGATGCAAGCGCCGTTGCTTACAAcagcag                                                                                                                     <  1:361441/140‑1 (MQ=255)                     tGAGCAGGATCGCGCACGTCGTTCCAACGAAGAACGCGGCAAACTGGTTACTCGTATCCAGACTGCTGTGAAATCCGTTGCCAACAGCCAGGATATCGATCTGGTTGTTGATGCAAGCGCCGTTGCTTACAACAGCAGCg                                                                                                                   <  2:1175145/140‑1 (MQ=255)                     tGAGCAGGATCGCGCACGTCGTTCCAACGAAGAACGCGGCAAACTGGTTACTCGTATCCAGACTGCTGTGAAATCCGTTGCCAACAGCCAGGATATCGATCTGGTTGTTGATGCAAGCGCCGTTGCTTACAACAGCAGCg                                                                                                                   <  1:891647/140‑1 (MQ=255)                     tGAGCAGGATCGCGCACGTCGTTCCAACGAAGAACGCGGCAAACTGGTTACTCGTATCCAGACTGCTGTGAAATCCGTTGCCAACAGCCAGGATATCGATCTGGTTGTTGATGCAAGCGCCGTTGCTTACAACAGCAGCg                                                                                                                   <  2:1033938/140‑1 (MQ=255)                            gATCGCGCACGTCGTTCCAACGAAGAACGCGGCAAACTGGTTACTCGTATCCAGACTGCTGTGAAATCCGTTGCCAACAGCCAGGATATCGATCTGGTTGTTGATGCAAGCGCCGTTGCTTACAACAGCAGCGATGTaaa                                                                                                            >  1:803623/1‑140 (MQ=255)                                  gcACGTCGTTCCAACGAAGAACGCGGCAAACTGGTTACTCGTATCCAGACTGCTGTGAAATCCGTTGCCAACAGCCAGGATATCGATCTGGTTGTTGATGCAAGCGCCGTTGCTTACAACAGCAGCGATGTAAAAGACAt                                                                                                      <  2:782274/140‑1 (MQ=255)                                  gcACGTCGTTCCAACGAAGAACGCGGCAAACTGGTTACTCGTATCCAGACTGCTGTGAAATCCGTTGCCAACAGCCAGGATATCGATCTGGTTGTTGATGCAAGCGCCGTTGCTTACAACAGCAGCGATGTAAAAGACAt                                                                                                      <  1:27566/140‑1 (MQ=255)                                           tCCAACGAAGAACGCGGCAAACTTGTTACTCGTATCCAGACTGCTGTGAAATCCGTTGCCAACAGCCAGGATATCGATCTGGTTGTTGATGCAAGCGCCGTTGCTTACAACAGCAGCGATGTAAAAGACATCACTGCCGa                                                                                             <  1:179491/140‑1 (MQ=255)                                           tCCAACGAAGAACGCGGCAAACTGGTTACTCGTATCCAGACTGCTGTGAAATCCGTTGCCAACAGCCAGGATATCGATCTGGTTGTTGATGCAAGCGCCGTTGCTTACAACAGCAGCGATGTAAAAGACATCACTGCCGa                                                                                             <  1:1335797/140‑1 (MQ=255)                                           tCCAACGAAGAACGCGGCAAACTGGTTACTCGTATCCAGACTGCTGTGAAATCCGTTGCCAACAGCCAGGATATCGATCTGGTTGTTGATGCAAGCGCCGTTGCTTACAACAGCAGCGATGTAAAAGACATCACTGCCGa                                                                                             <  2:383357/140‑1 (MQ=255)                                             cAACGAAGAACGCGGCAAACTGGTTACTCGTATCCAGACTGCTGTGAAATCCGTTGCCAACAGCCAGGATATCGATCTGGTTGTTGATGCAAGCGCCGTTGCTTACAACAGCAGCGATGTAAAAGACATCACTGCCGACg                                                                                           <  2:744539/140‑1 (MQ=255)                                                    gaaCGCGGCAAACTGGTTACTCGTATCCAGACTGCTGTGAAATCCGTTGCCAACAGCCAGGATATCGATCTGGTTGTTGATGCAAGCGCCGTTGCTTACAACAGCAGCGATGTAAAAGACATCACTGCCGACGTACTGaa                                                                                    <  2:218632/140‑1 (MQ=255)                                                     aaCGCGGCAAACTGGTTACTCGTATCCAGACTGCTGTGAAATCCGTTGCCAACAGCCAGGATATCGATCTGGTTGTTGATGCAAGCGCCGTTGCTTACAACAGCAGCGATGTAAAAGACATCACTGCCGACGTACTGaaa                                                                                   <  2:405808/140‑1 (MQ=255)                                                               aCTGGTTACTCGTATCCAGACTGCTGTGAAATCCGTTGCCAACAGCCAGGATATCGATCTGGTTGTTGATGCAAGCGCCGTTGCTTACAACAGCAGCGATGTAAAAGACATCACTGCCGACGTACTGAAACAGGTTAAAt                                                                         >  2:696463/1‑140 (MQ=255)                                                                      aCTCGTATCCAGACTGCTGTGAAATCCGTTGCCAACAGCCAGGATATCGATCTGGTTGTTGATGCAAGCGCCGTTGCTTACAACAGCAGCGATGTAAAAGACATCACTGCCGACGTACTGAAACAGGTTAAATAAGTAAt                                                                  <  1:406147/140‑1 (MQ=255)                                                                        tCGTATCCAGACTGCTGTGAAATCCGTTGCCAACAGCCAGGATATCGATCTGGTTGTTGATGCAAGCGCCGTTGCTTACAACAGCAGCGATGTAAAAGACATCACTGCCGACGTACTGAAACAGGTTAAATAAGTAATGc                                                                >  2:484890/1‑140 (MQ=255)                                                                         cGTATCCAGACTGCTGTGAAATCCGTTGCCAACAGCCAGGATATCGATCTGGTTGTTGATGCAAGCGCCGTTGCTTACAACAGCAGCGATGTAAAAGACATCACTGCCGACGTACTGAAACAGGTTAAATAAGTAATGcc                                                               <  2:1381512/140‑1 (MQ=255)                                                                           tATCCAGACTGCTGTGAAATCCGTTGCCAACAGCCAGGATATCGATCTGGTTGTTGATGCAAGCGCCGTTGCTTACAACAGCAGCGATGTAAAAGACATCACTGCCGACGTACTGAAACAGGTTAAATAAGTAATGCCtt                                                             >  2:181084/1‑140 (MQ=255)                                                                             tCCAGACTGCTGTGAAATCCGTTGCCAACAGCCAGGATATCGATCTGGTTGTTGATGCAAGCGCCGTTGCTTACAACAGCAGCGATGTAAAAGACATCACTGCCGACGTACTGAAACAGGTTAAATAAGTAATGCCTTCa                                                           >  2:763356/1‑140 (MQ=255)                                                                             tCCAGACTGCTGTGAAATCCGTTGCCAACAGCCAGGATATCGATCTGGTTGTTGATGCAAGCGCCGTTGCTTACAACAGCAGCGATGTAAAAGACATCACTGCCGACGTACTGAAACAGGTTAAATAAGTAATGCCTTCa                                                           >  1:740682/1‑140 (MQ=255)                                                                                  aCTGCTGTGAAATCCGTTGCCAACAGCCAGGATATCGATCTGGTTGTTGATGCAAGCGCCGTTGCTTACAACAGCAGCGATGTAAAAGACATCACTGCCGACGTACTGAAACAGGTTAAATAAGTAATGCCTTCAATTCg                                                      >  1:670987/1‑140 (MQ=255)                                                                                  aCTGCTGTGAAATCCGTTGCCAACAGCCAGGATATCGATCTGGTTGTTGATGCAAGCGCCGTTGCTTACAACAGCAGCGATGTAAAAGACATCACTGCCGACGTACTGAAACAGGTTAAATAAGTAATGCCTTCAATTCg                                                      >  2:917308/1‑140 (MQ=255)                                                                                  aCTGCTGTGAAATCCGTTGCCAACAGCCAGGATATCGATCTGGTTGTTGATGCAAGCGCCGTTGCTTACAACAGCAGCGATGTAAAAGACATCACTGCCGACGTACTGAAACAGGTTAAATAAGTAATGCCTTCAATTCg                                                      >  2:230774/1‑140 (MQ=255)                                                                                   ctgctgTGAAATCCGTTGCCAACAGCCAGGATATCGATCTGGTTGTTGATGCAAGCGCCGTTGCTTACAACAGCAGCGATGTAAAAGACATCACTGCCGACGTACTGAAACAGGTTAAATAAGTAATGCCTTCAATTCGa                                                     <  1:484890/140‑1 (MQ=255)                                                                                    tgctgGGAAATCCGTTACCAACAGCCAGGATATCGATCTGGTTGTTGATGCAAGCGCCGTTGCTTACAACAGCAGCGATGTAAAAGACATCACTGCCGACGTACTGAAACAGGTTAAATAAGTAATGCCTTCAATTCGAc                                                    >  2:279346/1‑140 (MQ=255)                                                                                      ctgTGAAATCCGTTGCCAACAGCCAGGATATCGATCTGGTTGTTGATGCAAGCGCCGTTGCTTACAACAGCAGCGATGTAAAAGACATCACTGCCGACGTACTGAAACAGGTTAAATAAGTAATGCCTTCAATTCGACTg                                                  >  2:330490/1‑140 (MQ=255)                                                                                       tgtgAAATCCGTTGCCAACAGCCAGGATATCGATCTGGTTGTTGATGCAAGCGCCGTTGCTTACAACAGCAGCGATGTAAAAGACATCACTGCCGACGTACTGAAACAGGTTAAATAAGTAATGCCTTCAATTCGACTgg                                                 >  2:692427/1‑140 (MQ=255)                                                                                        gtgAAATCCGTTGCCAACAGCCAGGATATCGATCTGGTTGTTGATGCAAGCGCCGTTGCTTACAACAGCAGCGATGTAAAAGACATCACTGCCGACGTACTGAAACAGGTTAAATAAGTAATGCCTTCAATTCGACTGGc                                                <  1:1386681/140‑1 (MQ=255)                                                                                           aaaTCCGTTGCCAACAGCCAGGATATCGATCTGGTTGTTGATGCAAGCGCCGTTGCTTACAACAGCAGCGATGTAAAAGACATCACTGCCGACGTACTGAAACAGGTTAAATAAGTAATGCCTTCAATTCGACTGGCTGa                                             <  2:803623/140‑1 (MQ=255)                                                                                                          aGCCAGGATATCGATCTGGTTGTTGATGCAAGCGCCGTTGCTTACAACAGCAGCGATGTAAAAGACATCACTGCCGACGTACTGAAACAGGTTAAATAAGTAATGCCTTCAATTCGACTGGCTGATTTAGCGCAGCAGtt                              <  1:330490/140‑1 (MQ=255)                                                                                                          aGCCAGGATATCGATCTGGTTGTTGATGCAAGCGCCGTTGCTTACAACAGCAGCGATGTAAAAGACATCACTGCCGACGTACTGAAACAGGTTAAATAAGTAATGCCTTCAATTCGACTGGCTGATTTAGCGCAGCAGtt                              <  1:834901/140‑1 (MQ=255)                                                                                                             cAGGATATCGATCTGGTTGTTGATGCAAGCGCCGTTGCTTACAACAGCAGCGATGTAAAAGACATCACTGCCGACGTACTGAAACAGGTTAAATAAGTAATGCCTTCAATTCGACTGGCTGATTTAGCGCAGCAGTTGGa                           <  1:279346/140‑1 (MQ=255)                                                                                                               ggATATCGATCTGGTTGTTGATGCAAGCGCCGTTGCTTACAACAGCAGCGATGTAAAAGACATCACTGCCGACGTACTGAAACAGGTTAAATAAGTAATGCCTTCAATTCGACTGGCTGATTTAGCGCAGCAGTTGGATg                         <  1:763356/140‑1 (MQ=255)                                                                                                                  tatCGATCTGGTTGTTGATGCAAGCGCCGTTGCTTACAACAGCAGCGATGTAAAAGACATCACTGCCGACGTACTGAAACAGGTTAAATAAGTAATGCCTTCAATTCGACTGGCTGATTTAGCGCAGCAGTTGGATGCAg                      <  2:744347/140‑1 (MQ=255)                                                                                                                    tCGATCTGGTTGTTGATGCAAGCGCCGTTGCTTACAACAGCAGCGATGTAAAAGACATCACTGCCGACGTACTGAAACAGGTTAAATAAGTAATGCCTTCAATTCGACTGGCTGATTTAgcg                                      <  2:870329/122‑1 (MQ=255)                                                                                                                    tCGATCTGGTTGTTGATGCAAGCGCCGTTGCTTACAACAGCAGCGATGTAAAAGACATCACTGCCGACGTACTGAAACAGGTTAAATAAGTAATGCCTTCAATTCGACTGGCTGATTTAgcg                                      >  1:870329/1‑122 (MQ=255)                                                                                                                    tCGATCTGGTTGTTGATGCAAGCGCCGTTGCTTACAACAGCAGCGATGTAAAAGACATCACTGCCGACGTACTGAAACAGGTTAAATAAGTAATGCCTTCAATTCGACTGGCTGATTTAGCGCAGCAGTTGGATGCAGaa                    >  2:265336/1‑140 (MQ=255)                                                                                                                    tCGATCTGGTTGTTGATGCAAGCGCCGTTGCTTACAACAGCAGCGATGTAAAAGACATCACTGCCGACGTACTGAAACAGGTTAAATAAGTAATGCCTTCAATTCGACTGGCTGATTTAGCGCAGCAGTTGGATGCAGaa                    >  2:882700/1‑140 (MQ=255)                                                                                                                         cTGGTTGTTGATGCAAGCGCCGTTGCTTACAACAGCAGCGATGTAAAAGACATCACTGCCGACGTACTGAAACAGGTTAAATAAGTAATGCCTTCAATTCGACTGGCTGATTTAGCGCAGCAGTTGGATGCAGAACTaca               <  2:1398280/140‑1 (MQ=255)                                                                                                                          tGGTTGTTGATGCAAGCGCCGTTGCTTACAACAGCAGCGATGTAAAAGACATCACTGCCGACGTACTGAAACAGGTTAAATAAGTAATGCCTTCAATTCGACTGGCTGATTTAGCGCAGCAGTTGGATGCAg                      >  2:983538/1‑132 (MQ=255)                                                                                                                          tGGTTGTTGATGCAAGCGCCGTTGCTTACAACAGCAGCGATGTAAAAGACATCACTGCCGACGTACTGAAACAGGTTAAATAAGTAATGCCTTCAATTCGACTGGCTGATTTAGCGCAGCAGTTGGATGCAg                      <  1:983538/132‑1 (MQ=255)                                                                                                                          tGGTTGTTGATGCAAGCGCCGTTGCTTACAACAGCAGCGATGTAAAAGACATCACTGCCGACGTACTGAAACAGGTTAAATAAGTAATGCCTTCAATTCGACTGGCTGATTTAGCGCAGCAGTTGGATGCAGAACTacac              >  2:140659/1‑140 (MQ=255)                                                                                                                           ggTTGTTGATGCAAGCGCCGTTGCTTACAACAGCAGCGATGTAAAAGACATCACTGCCGACGTACTGAAACAGGTTAAATAAGTAATGCCTTCAATTCGACTGGCTGATTTAGCGCAGCAGTTGGATGCAGAACTACACg             >  2:595285/1‑140 (MQ=255)                                                                                                                            gttgttGATGCAAGCGCCGTTGCTTACAACAGCAGCGATGTAAAAGACATCACTGCCGACGTACTGAAACAGGTTAAATAAGTAATGCCTTCAATTCGACTGGCTGATTTAGCGCAGCAGTTGGATGCAGAACTACACgg            >  2:1260154/1‑140 (MQ=255)                                                                                                                              tgttgATGCAAGCGCCGTTGCTTACAACAGCAGCGATGTAAAAGACATCACTGCCGACGTACTGAAACAGGTTAAATAAGTAATGCCTTCAATTCGACTGGCTGATTTAGCGCAGCAGTTGGATGCAGAACTACACGGTg          >  2:536872/1‑140 (MQ=255)                                                                                                                                  gATGCAAGCGCCGTTGCTTACAACAGCAGCGATGTAAAAGACATCACTGCCGACGTACTGAAACAGGTTAAATAAGTAATGCCTTCAATTCGACTGGCTGATTTAGCGCAGCAGTTGGATGCAGAACTACACGGTGATgg      >  2:340167/1‑140 (MQ=255)                                                                                                                                  gATGCAAGCGCCGTTGCTTACAACAGCAGCGATGTAAAAGACATCACTGCCGACGTACTGAAACAGGTTAAATAAGTAATGCC‑TCAATTCGACTGGCTGATTTAGCGCAGCAGTTGGATGCAGAACTACACGGTGATGGc     >  2:1097391/1‑140 (MQ=255)                                                                                                                                    tGCAAGCGCCGTTGCTTACAACAGCAGCGATGTAAAAGACATCACTGCCGACGTACTGAAACAGGTTAAATAAGTAATGCCTTCAATTCGACTGGCTGATTTAGCGCAGCAGTTGGATGCAGAACTACACGGTGATGGCg    <  1:692427/140‑1 (MQ=255)                                                                                                                                      cAAGCGCCGTTGCTTACAACAGCAGCGATGTAAAAGACATCACTGCCGACGTACTGAAACAGGTTAAATAAGTAATGCCTTCAATTCGACTGGCTGATTTAGCGCAGCAGTTGGATGCAGAACTACACGGTGATGGCGat  <  1:549590/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                         GCTCAGAAAGCGCAGGCTTTTGAGCAGGATCGCGCACGTCGTTCCAACGAAGAACGCGGCAAACTGGTTACTCGTATCCAGACTGCTGTGAAATCCGTTGCCAACAGCCAGGATATCGATCTGGTTGTTGATGCAAACGCCGTTGCTTACAACAGCAGCGATGTAAAAGACATCACTGCCGACGTACTGAAACAGGTTAAATAAGTAATGCCTTCAATTCGACTGGCTGATTTAGCGCAGCAGTTGGATGCAGAACTACACGGTGATGGCGAT  >  NC_000913/200764‑201036

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑