Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,701,635	T→C	L227L (TTG→CTG)	malY →	negative regulator of MalT activity/cystathionine beta‑lyase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,701,635	0	T	C	100.0%	211.9 / NA	64	L227L (TTG→CTG)	malY	negative regulator of MalT activity/cystathionine beta‑lyase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (36/28);  total (36/28)

CGATGAGCTGGAGATCATGGCTGACCTGTGCGAGCGTCATGGTGTGCGGGTTATTTCCGATGAAATCCATATGGATATGGTTTGGGGCGAGCAGCCGCATATTCCCTGGAGTAATGTGGCTCGCGGAGACTGGGCGTTGCTAACGTCGGGCTCGAAAAGTTTCAATATTCCCGCCCTGACCGGTGCTTACGGGATTATAGAAAATAGCAGTAGCCGCGATGCCTATTTATCGGCACTGAAAGGCCGTGATGGGCTTTCTTCCCCTTCGGTACT  >  NC_000913/1701499‑1701771                                                                                                                                         |                                                                                                                                         cGATGAGCTGGAGATCATGGCTGACCTGTGCGAGCGTCATGGTGTGCGGGTTATTTCCGATGAAATCCATATGGATATGGTTTGGGGCGAGCAGCCGCATATTCCCTGGAGTAATGTGGCTCGCGGAGACTGGGCGCTGc                                                                                                                                       >  1:969127/1‑140 (MQ=255) cGATGAGCTGGAGATCATGGCTGACCTGTGCGAGCGTCATGGTGTGCGGGTTATTTCCGATGAAATCCATATGGATATGGTTTGGGGCGAGCAGCCGCATATTCCCTGGAGTAATGTGGCTCGCGGAGACTGGGCGCTGc                                                                                                                                       >  1:184399/1‑140 (MQ=255) cGATGAGCTGGAGATCATGGCTGACCTGTGCGAGCGTCATGGTGTGCGGGTTATTTCCGATGAAATCCATATGGATATGGTTTGGGGCGAGCAGCCGCATATTCCCTGGAGTAATGTGGCTCGCGGAGACTGGGCGCTGc                                                                                                                                       >  2:1202477/1‑140 (MQ=255)      aGCTGGAGATCATGGCTGACCTGTGCGAGCGTCATGGTGTGCGGGTTATTTCCGATGAAATCCATATGGATATGGTTTGGGGCGAGCAGCCGCATATTCCCTGGAGTAATGTGGCTCGCGGAGACTGGGCGCTGCTAACg                                                                                                                                  >  1:645005/1‑140 (MQ=255)        cTGGAGATCATGGCTGACCTGTGCGAGCGTCATGGTGTGCGGGTTATTTCCGATGAAATCCATATGGATATGGTTTGGGGCGAGCAGCCGCATATTCCCTGGAGTAATGTGGCTCGCGGAGACTGGGCGCTGCTAACGTc                                                                                                                                >  1:1218266/1‑140 (MQ=255)        cTGGAGATCATGGCTGACCTGTGCGAGCGTCATGGTGTGCGGGTTATTTCCGATGAAATCCATA‑GGATATGGTTTGGGGCGAGCAGCCGCATATTCCCTGGAGTAATGTGGCTCGCGGAGACTGGGCGCTGCTAACGTCg                                                                                                                               >  2:116498/1‑140 (MQ=255)                cATGGCTGACCTGTGCGAGCGTCATGGTGTGCGGGTTATTTCCGATGAAATCCATATGGATATGGTTTGGGGCGAGCAGCCGCATATTCCCTGGAGTAATGTGGCTCGCGGAGACTGGGCGCTGCTAACGTCGGGCTCGa                                                                                                                        >  1:924156/1‑140 (MQ=255)                cATGGCTGACCTGTGCGAGCGTCATGGTGTGCGGGTTATTTCCGATGAAATCCATATGGATATGGTTTGGGGCGAGCAGCCGCATATTCCCTGGAGTAATGTGGCTCGCGGAGACTGGGCGCTGCTAACGTCGGGCTCGa                                                                                                                        >  1:734191/1‑140 (MQ=255)                cATGGCTGACCTGTGCGAGCGTCATGGTGTGCGGGTTATTTCCGATGAAATCCATATGGATATGGTTTGGGGCGAGCAGCCGCATATTCCCTGGAGTAATGTGGCTCGCGGAGACTGGGCGCTGCTAACGTCGGGCTCGa                                                                                                                        >  2:508378/1‑140 (MQ=255)                     cTGACCTGTGCGAGCGTCATGGTGTGCGGGTTATTTCCGATGAAATCCATATGGATATGGTTTGGGGCGAGCAGCCGCATATTCCCTGGAGTAATGTGGCTCGCGGAGACTGGGCGCTGCTAACGTCGGGCTCGAAAAGt                                                                                                                   >  2:419989/1‑140 (MQ=255)                     cTGACCTGTGCGAGCGTCATGGTGTGCGGGTTATTTCCGATGAAATCCATATGGATATGGTTTGGGGCGAGCAGCCGCATATTCCCTGGAGTAATGTGGCTCGCGGAGACTGGGCGCTGCTAACGTCGGGCTCGAAAAGt                                                                                                                   >  1:620299/1‑140 (MQ=255)                     cTGACCTGTGCGAGCGTCATGGTGTGCGGGTTATTTCCGATGAAATCCATATGGATATGGTTTGGGGCGAGCAGCCGCATATTCCCTGGAGTAATGTGGCTCACGGAGACTGGGCGCTGCTAACGTCGGGCTCGAAAAGt                                                                                                                   >  2:748296/1‑140 (MQ=255)                         ccTGTGCGAGCGTCATGGTGTGCGGGTTATTTCCGATGAAATCCATATGGATATGGTTTGGGGCGAGCAGCGGCATATTCCCTGGAGTAATGTGGCTCGCGGAGACTGGGCGCTGCTAACGTCGGGCTCGAAAAGTTTCa                                                                                                               >  2:870819/1‑140 (MQ=255)                          cTGTGCGAGCGTCATGGTGTGCGGGTTATTTCCGATGAAATCCATATGGATATGGTTTGGGGCGAGCAGCCGCATATTCCCTGGAGTAATGTGGCTCGCGGAGACTGGGCGCTGCTAACGTCGGGCTCGAAAAGTTTCaa                                                                                                              <  2:734191/140‑1 (MQ=255)                          cTGTGCGAGCGTCATGGTGTGCGGGTTATTTCCGATGAAATCCATATGGATATGGTTTGGGGCGAGCAGCCGCATATTCCCTGGAGTAATGTGGCTCGCGGAGACTGGGCGCTGCTAACGTCGGGCTCGAAAAGTTTCaa                                                                                                              <  1:406362/140‑1 (MQ=255)                          cTGTGCGAGCGTCATGGTGTGCGGGTTATTTCCGATGAAATCCATATGGATATGGTTTGGGGCGAGCAGCCGCATATTCCCTGGAGTAATGTGGCTCGCGGAGACTGGGCGCTGCTAACGTCGGGCTCGAAAAGTTTCaa                                                                                                              >  1:1208909/1‑140 (MQ=255)                                 aGCGTCATGGTGTGCGGGTTATTTCCGATGAAATCCATATGGATATGGTTTGGGGCGAGCAGCCGCATATTCCCTGGAGTAATGTGGCTCGCGGAGACTGGGCGCTGCTAACGTCGGGCTCGAAAAGTTTCAATATTccc                                                                                                       <  2:184399/140‑1 (MQ=255)                                 aGCGTCATGGTGTGCGGGTTATTTCCGATGAAATCCATATGGATATGGTTTGGGGCGAGCAGCCGCATATTCCCTGGAGTAATGTGGCTCGCGGAGACTGGGCGCTGCTAACGTCGGGCTCGAAAAGTTTCAATATTccc                                                                                                       <  1:340267/140‑1 (MQ=255)                                   cGTCATGGTGTGCGGGTTATTTCCGATGAAATCCATATGGATATGGTTTGGGGCGAGCAGCCGCATATTCCCTGGAGTAATGTGGCTCGCGGAGACTGGGCGCTGCTAACGTCGGGCTCGAAAAGTTTCAATATTCCCGc                                                                                                     >  2:713003/1‑140 (MQ=255)                                    gTCATGGTGTGCGGGTTATTTCCGATGAAATCCATATGGATATGGTTTGGGGCGAGCAGCCGCATATTCCCTGGAGTAATGTGGCTCGCGGAGACTGGGCGCTGCTAACGTCGGGCTCGAAAAGTTTCAATATTCCCGcc                                                                                                    <  1:508378/140‑1 (MQ=255)                                    gTCATGGTGTGCGGGTTATTTCCGATGAAATCCATATGGATATGGTTTGGGGCGAGCAGCCGCATATTCCCTGGAGTAATGTGGCTCGCGGAGACTGGGCGCTGCTAACGTCGGGCTCGAAAAGTTTCAATATTCCCGcc                                                                                                    >  1:619129/1‑140 (MQ=255)                                      cATGGTGTGCGGGTTATTTCCGATGAAATCCATATGGATATGGTTTGGGGCGAGCAGCCGCATATTCCCTGGAGTAATGTGGCTCGCGGAGACTGGGCGCTGCTAACGTCGGGCTCGAAAAGTTTCAATATTCCCGCCCt                                                                                                  <  1:987389/140‑1 (MQ=255)                                         ggTGTGCGGGTTATTTCCGATGAAATCCATATGGATATGGTTTGGGGCGAGCAGCCGCATATTCCCTGGAGTAATGTGGCTCGCGGAGACTGGGCGCTGCTAACGTCGGGCTCGAAAAGTTTCAATATTCCCGCCCTGAc                                                                                               <  2:339323/140‑1 (MQ=255)                                            gtgCGGGTTATTTCCGATGAAATCCATATGGATATGGTTTGGGGCGAGCAGCCGCATATTCCCTGGAGTAATGTGGCTCGCGGAGACTGGGCGCTGCTAACGTCGGGCTCGAAAAGTTTCAATATTCCCGCCCTGACCgg                                                                                            >  1:1408779/1‑140 (MQ=255)                                                  gTTATTTCCGATGAAATCCATATGGATATGGTTTGGGGCGAGCAGCCGCATATTCCCTGGAGTAATGTGGCTCGCGGAGACTGGGCGCTGCTAACGTCGGGCTCGAAAAGTTTCAATATTCCCGCCCTGACCGGTGCTTa                                                                                      <  1:419989/140‑1 (MQ=255)                                                  gTTATTTCCGATGAAATCCATATGGATATGGTTTGGGGCGAGCAGCCGCATATTCCCTGGAGTAATGTGGCTCGCGGAGACTGGGCGCTGCTAACGTCGGGCTCGAAAAGTTTCAATATTCCCGCCCTGACCGGTGCTTa                                                                                      <  2:1208909/140‑1 (MQ=255)                                                  gTTATTTCCGATGAAATCCATATGGATATGGTTTGGGGCGAGCAGCCGCATATTCCCTGGAGTAATGTGGCTCGCGGAGACTGGGCGCTGCTAACGTCGGGCTCGAAAAGTTTCAATATTCCCGCCCTGACCGGTGCTTa                                                                                      >  1:608404/1‑140 (MQ=255)                                                  gTTATTTCCGATGAAATCCATATGGATATGGTTTGGGGCGAGCAGCCGCATATTCCCTGGAGTAATGTGGCTCGCGGAGACTGGGCGCTGCTAACGTCGGGCTCGAAAAGTTTCAATATTCCCGCCCTGACCGGTGCTTa                                                                                      <  2:608404/140‑1 (MQ=255)                                                  gTTATTTCCGATGAAATCCATATGGATATGGTTTGGGGCGAGCAGCCGCATATTCCCTGGAGTAATGTGGCTCGCGGAGACTGGGCGCTGCTAACGTCGGGCTCGAAAAGTTTCAATATTCCCGCCCTGACCGGTGCTTa                                                                                      <  1:1090857/140‑1 (MQ=255)                                                    tATTTCCGATGAAATCCATATGGATATGGTTTGGGGCGAGCAGCCGCATATTCCCTGGAGTAATGTGGCTCGCGGAGACTGGGCGCTGCTAACGTCGGGCTCGAAAAGTTTCAATATTCCCGCCCTGACCGGTGCTTACg                                                                                    >  1:1016766/1‑140 (MQ=255)                                                     aTTTCCGATGAAATCCATATGGATATGGTTTGGGGCGAGCAGCGGCATATTCCCTGGAGTAATGTGGCTCGCGGAGACTGGGCGCTGCTAACGTCGGGCTCGAAAAGTTTCAATATTCCCGCCCTGACCGGTGCTTACgg                                                                                   <  1:870819/140‑1 (MQ=255)                                                     aTTTCCGATGAAATCCATATGGATATGGTTTGGGGCGAGCAGCCGCATATTCCCTGGAGTAATGTGGCTCGCGGAGACTGGGCGCTGCTAACGTCGGGCTCGAAAAGTTTCAATATTCCCGCCCTGACCGGTGCTTACgg                                                                                   <  2:969127/140‑1 (MQ=255)                                                       ttCCGATGAAATCCATATGGATATGGTTTGGGGCGAGCAGCCGCATATTCCCTGGAGTAATGTGGCTCGCGGAGACTGGGCGCTGCTAACGTCGGGCTCGAAAAGTTTCAATATTCCCGCCCTGACCGGTGCTTACGGGa                                                                                 <  2:199101/140‑1 (MQ=255)                                                       ttCCGATGAAATCCATATGGATATGGTTTGGGGCGAGCAGCCGCATATTCCCTGGAGTAATGTGGCTCGCGGAGACTGGGCGCTGCTAACGTCGGGCTCGAAAAGTTTCAATATTCCCGCCCTGACCGGTGCTTACGGGa                                                                                 <  2:924156/140‑1 (MQ=255)                                                       ttCCGATGAAATCCATATGGATATGGATTGGGGCGAGCAGCCGCATATTCCCTGGAGTAATGTGGCTCTCGGAGACTTGGCGCAGACAACGCCGGGGCCGCAAAGGATTAATATTCCCGCCCTGACCGGTGCTTACgggg                                                                                 >  2:636035/1‑139 (MQ=255)                                                                             atggTTTGGGGCGAGCAGCCGCATATTCCCTGGAGTAATGTGGCTCGCGGAGACTGGGCGCTGCTAACGTCGGGCTCGAAAAGTTTCAATATTCCCGCCCTGACCGGTGCTTACGGGATTATAGAAAATAGCAGTAGCcg                                                           <  2:1185593/140‑1 (MQ=255)                                                                              tggTTTGGGGCGAGCAGCCGCATATTCCCTGGAGTAATGTGGCTCGCGGAGACTGGGCGCTGCTAACGTCGGGCTCGAAAAGTTTCAATATTCCCGCCCTGACCGGTGCTTACGGGATTATAGAAAATAGCAGTAGCcgc                                                          >  1:1342251/1‑140 (MQ=255)                                                                                gTTTGGGGCGAGCAGCCGCATATTCCCTGGAGTAATGTGGCTCGCGGAGACTGGGCGCTGCTAACGTCGGGCTCGAAAAGTTTCAATATTCCCGCCCTGACCGGTGCTTACGGGATTATAGAAAATAGCAGTAGCCGCGa                                                        <  1:863927/140‑1 (MQ=255)                                                                                 tttGGGGCGAGCAGCCGCATATTCCCTGGAGTAATGTGGCTCGCGGAGACTGGGCGCTGCTAACGTCGGGCTCGAAAAGTTTCAATATTCCCGCCCTGACCGGTGCTTACGGGATTATAGAAAATAGCAGTAGCCGCGAt                                                       <  1:926555/140‑1 (MQ=255)                                                                                   tGGGGCGAGCAGCCGCATATTCCCTGGAGTAATGTGGCTCGCGGAGACTGGGCGCTGCTAACGTCGGGCTCGAAAAGTTTCAATATTCCCGCCCTGACCGGTGCTTACGGGATTATAGAAAATAGCAGTAGCCGCGATGc                                                     >  2:188321/1‑140 (MQ=255)                                                                                   tGGGGCGAGCAGCCGCATATTCCCTGGAGTAATGTGGCTCGCGGAGACTGGGCGCTGCTAACGTCGGGCTCGAAAAGTTTCAATATTCCCGCCCTGACCGGTGCTTACGGGATTATAGAAAATAGCAGTAGCCGCGATGc                                                     >  2:806159/1‑140 (MQ=255)                                                                                   tGGGGCGAGCAGCCGCATATTCCCTGGAGTAATGTGGCTCGCGGAGACTGGGCGCTGCTAACGTCGGGCTCGAAAAGTTTCAATATTCCCGCCCTGACCGGGGCTTACGGGATTATAGAAAAAAGCAGTAGCCGCGATGc                                                     >  2:1184465/1‑140 (MQ=255)                                                                                    ggggCGAGCAGCCGCATATTCCCTGGAGTAATGTGGCTCGCGGAGACTGGGCGCTGCTAACGTCGGGCTCGAAAAGTTTCAATATTCCCGCCCTGACCGGTGCTTACGGGATTATAGAAAATAGCAGTAGCCGCGATGcc                                                    <  2:935550/140‑1 (MQ=255)                                                                                     gggCGAGCAGCCGCATATTCCCTGGAGTAATGTGGCTCGCGGAGACTGGGCGCTGCTAACGTCGGGCTCGAAAAGTTTCAATATTCCCGCCCTGACCGGTGCTTACGGGATTATAGAAAATAGCAGTAGCCGCGATGCCt                                                   >  2:1242938/1‑140 (MQ=255)                                                                                     gggCGAGCAGCCGCATATTCCCTGGAGTAATGTGGCTCGCGGAGACTGGGCGCTGCTAACGTCGGGCTCGAAAAGTTTCAATATTCCCGCCCTGACCGGTGCTTACGGGATTATAGAAAATAGCAGTAGCCGCGATGCCt                                                   >  1:562603/1‑140 (MQ=255)                                                                                          agcagcCGCATATTCCCTGGAGTAATGTGGCTCGCGGAGACTGGGCGCTGCTAACGTCGGGCTCGAAAAGTTTCAATATTCCCGCCCTGACCGGTGCTTACGGGATTATAGAAAATAGCAGTAGCCGCGATGCCTATTTa                                              >  1:553235/1‑140 (MQ=255)                                                                                          agcagcCGCATATTCCCTGGAGTAATGTGGCTCGCGGAGACTGGGCGCTGCTAACGTCGGGCTCGAAAAGTTTCAATATTCCCGCCCTGACCGGTGCTTACGGGATTATAGAAAATAGCAGTAGCCGCGATGCCTATTTa                                              >  2:534415/1‑140 (MQ=255)                                                                                           gcagcCGCATATTCCCTGGAGTAATGTGGCTCGCGGAGACTGGGCGCTGCTAACGTCGGGCTCGAAAAGTTTCAATATTCCCGCCCTGACCGGTGCTTACGGGATTATAGAAAATAGCAGTAGCCGCGATGCCTATTTAt                                             <  2:1408779/140‑1 (MQ=255)                                                                                           gcagcCGCATATTCCCTGGAGTAATGTGGCTCGCGGAGACTGGGCGCTGCTAACGTCGGGCTCGAAAAGTTTCAATATTCCCGCCCTGACCGGTGCTTACGGGATTATAGAAAATAGCAGTAGCCGCGATGCCTATTTAt                                             <  1:973121/140‑1 (MQ=255)                                                                                           gcagcCGCATATTCCCTGGAGTAATGTGGCTCGCGGAGACTGGGCGCTGCTAACGTCGGGCTCGAAAAGTTTCAATATTCCCGCCCTGACCGGTGCTTACGGGATTATAGAAAATAGCAGTAGCCGCGATGCCTATTTAt                                             <  2:597076/140‑1 (MQ=255)                                                                                              gcCGCATATTCCCTGGAGTAATGTGGCTCGCGGAGACTGGGCGCTGCTAACGTCGGGCTCGAAAAGTTTCAATATTCCCGCCCTGACCGGTGCTTACGGGATTATAGAAAATAGCAGTAGCCGCGATGCCTATTTATCgg                                          <  2:415224/140‑1 (MQ=255)                                                                                                    tatTCCCTGGAGTAATGTGGCTCGCGGAGACTGGGCGCTGCTAACGTCTGGGTCGAAAAGTTTCAATATTCCCGCCCCGACCAGAGCTTTCGGGATTATAAAAAAAAGCAGTAGCCGCGATGCCTATTTATCGGCACTGa                                    >  2:973121/1‑140 (MQ=255)                                                                                                            ggAGTAATGTGGCTCGCGGAGACTGGGCGCTGCTAACGTCGGGCTCGAAAAGTTTCAATATTTCCGCCCTGACCGGTGCTTACGGGATTATAGAAAATAGCAGTAACCGCGATGCCTATTTATCGGCACTGAAAGGCCGt                            >  1:331410/1‑140 (MQ=255)                                                                                                            gcactAATGTGCCTCGCGGAGACTGGGCGCTGCTAACGTCGCGCTCGAAAAGTTCCAATATTCCCGCCCTGACCGGTGCTTACGCGATTATAGAAAATAGCAGTAACCCCGATGCCTATTTATCGCCACTGAAAGGCCGt                            <  2:1218266/136‑1 (MQ=255)                                                                                                              aGTAATGTGGCTCGCGGAGACTGGGCGCTGCTAACGTCGGGCTCGAAAAGTTTCAATATTCCCGCCCTGACCGGTGCTTACGGGATTATAGAAAATAGCAGTAGCCGCGATGCCTATTTATCGGCACTGAAAGGCCGTGa                          >  2:240230/1‑140 (MQ=255)                                                                                                                  aTGTGGCTCGCGGAGACTGGGCGCTGCTAACGTCGGGCTCGAAAAGTTTCAATATTCCCGCCCTGACCGGTGCTTACGGGATTATAGAAAATAGCAGTAGCCGCGATGCCTATTTATCGGCACTGAAAGGCCGTGATggg                      >  2:1269444/1‑140 (MQ=255)                                                                                                                  aTGTGGCTCGCGGAGACTGGGCGCTGCTAACGTCGGGCTCGAAAAGTTTCAATATTCCCGCCCTGACCGGTGCTTACGGGATTATAGAAAATAGCAGTAGCCGCGATGCCTATTTATCGGCACTGAAAGGCCGTGATggg                      <  1:1202477/140‑1 (MQ=255)                                                                                                                   tgtgGCTCGCGGAGACTGGGCGCTGCTAACGTCGGGCTCGAAAAGTTTCAATATTCCCGCCCTGACCGGTGCTTACGGGATTATAGAAAATAGCAGTAGCCGCGATGCCTATTTATCGGCACTGAAAGGCCGTGATGGGc                     <  2:553235/140‑1 (MQ=255)                                                                                                                       gCTCGCGGAGACTGGGCGCTGCTAACGTCGGGCTCGAAAAGTTTCAATATTCCCGCCCTGACCGGTGCTTACGGGATTATAGAAAATAGCAGTAGCCGCGATGCCTATTTATCGGCACTGAAAGGCCGTGATGGGCTttc                 >  1:682796/1‑140 (MQ=255)                                                                                                                        cTCGCGGAGACTGGGCGCTGCTAACGTCGGGCTCGAAAAGTTTCAATATTCCCGCCCTGACCGGTGCTTACGGGATTATAGAAAATAGCAGTAGCCGCGATGCCTATTTATCGGCACTGAAAGGCCGTGATGGGCTttct                >  2:980453/1‑140 (MQ=255)                                                                                                                           gcgGAGACTGGGCGCTGCTAACGTCGGGCTCGAAAAGTTTCAATACTCCCGCCCTGACCGGTGCTTACGGGATTATAGAAAATAGCAGTAGCCGCGATGCCTATTTATCGGCACTGAAAGGCCGTGATGGGCTTTCTTcc             <  2:176323/140‑1 (MQ=255)                                                                                                                            cgGAGACTGGGCGCTGCTAACGTCGGGCTCGAAAAGTTTCAATATTCCCGCCCTGACCGGTGCTTACGGGATTATAGAAAATAGCAGTAGCCGCGATGCCTATTTATCGGCACTGAAAGGCCGTGATGGGCTTTCTTccc            >  2:715350/1‑140 (MQ=255)                                                                                                                            cgGAGACTGGGCGCTGCTAACGTCGGGCTCGAAAAGTTTCAATATTCCCGCCCTGACCGGTGCTTACGGGATTATAGAAAATAGCAGTAGCCGCGATGCCTATTTATCGGCACTGAAAGGCCGTGATGGGCTTTCTTccc            >  1:488518/1‑140 (MQ=255)                                                                                                                               agaCTGGGCGCTGCTAACGTCGGGCTCGAAAAGTTTCAATATTCCCGCCCTGACCGGTGCTTACGGGATTATAGAAAATAGCAGTAGCCGCGATGCCTATTTATCGGCACTGAAAGGCCGTGATGGGCTTTCTTCCCCtt         <  2:619129/140‑1 (MQ=255)                                                                                                                                 aCTGGGCGCTGCTAACGTCGGGCTCGAAAAGTTTCAATATTCCCGCCCTGACCGGTGCTTACGGGATTATAGAAAATAGCAGTAGCCGCGATGCCTATTTATCGGCACTGAAAGGCCGTGATGGGCTTTCTTCCCCTTCg       >  1:1360456/1‑140 (MQ=255)                                                                                                                                    gggCGCTGCTAACGTCGGGCTCGAAAAGTTTCAATATTCCCGCCCTGACCGGTGCTTACGGGATTATAGAAAATAGCAGTAGCCGCGATGCCTATTTATCGGCACTGAAAGGCCGTGATGGGCTTTCTTCCCCTTCGGTa    <  1:116498/140‑1 (MQ=255)                                                                                                                                     ggCGCTGCTAACGTCGGGCTCGAAAAGTTTCAATATTCCCGCCCTGACCGGTGCTTACGGGATTATAGAAAATAGCAGTAGCCGCGATGCCTATTTATCGGCACTGAAAGGCCGTGATGGGCTTTCTTCCCCTTCGGTAc   >  1:519608/1‑140 (MQ=255)                                                                                                                                     ggCGCTGCTAACGTCGGGCTCGAAAAGTTTCAATATTCCCGCCCTGACCGGTGCTTACGGGATTATAGAAAATAGCAGTAGCCGCGATGCCTATTTATCGGCACTGAAAGGCCGTGATGGGCTTTCTTCCCCTTCGGTAc   >  1:1063019/1‑140 (MQ=255)                                                                                                                                      gcgcTGCTAACGTCGGGCTCGAAAAGTTTCAATATTCCCGCCCTGACCGGTGCTTACGGGATTATAGAAAATAGCAGTAGCCGCGATGCCTATTTATCGGCACTGAAAGGCCGTGATGGGCTTTCTTCCCCTTCGGTACt  <  1:636035/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                         CGATGAGCTGGAGATCATGGCTGACCTGTGCGAGCGTCATGGTGTGCGGGTTATTTCCGATGAAATCCATATGGATATGGTTTGGGGCGAGCAGCCGCATATTCCCTGGAGTAATGTGGCTCGCGGAGACTGGGCGTTGCTAACGTCGGGCTCGAAAAGTTTCAATATTCCCGCCCTGACCGGTGCTTACGGGATTATAGAAAATAGCAGTAGCCGCGATGCCTATTTATCGGCACTGAAAGGCCGTGATGGGCTTTCTTCCCCTTCGGTACT  >  NC_000913/1701499‑1701771

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑