Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,781,212	A→G	N271S (AAC→AGC)	ydiR →	putative electron transfer flavoprotein subunit YdiR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,781,212	0	A	G	100.0%	130.8 / NA	39	N271S (AAC→AGC)	ydiR	putative electron transfer flavoprotein subunit YdiR
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (23/16);  total (23/16)

AATTGCCGAAGGCGAGAACTGGATGGAGCGTGAACGTTATATCGGTGTCTCCGGCGTGTTGCTGAAATCCGATCTCTACCTGACGCTGGGGATCTCCGGGCAGATCCAGCATATGGTTGGCGGCAACGGCGCAAAAGTGATTGTCGCCATCAATAAAGATAAAAATGCGCCAATCTTCAACTATGCCGACTACGGTCTGGTGGGCGATATCTACAAAGTCGTCCCTGCCCTGATTAGCCAGTTGAGCCGCTAATTCCC  >  NC_000913/1781087‑1781344                                                                                                                              |                                                                                                                                     aaTTGCCGAAGGCGAGAACTGGATGGAGCGTGAACGTTATATCGGTGTCTCCGGCGTGTTGCTGAAATCCGATCTCTACCTGACGCTGGGGATCTCCGGGCAGATCCAGCATATGGTTGGCGGCAGCGGCGCAAAAGTGa                                                                                                                        <  2:959853/140‑1 (MQ=255)  aTTGCCGAAGGCGAGAACTGGATGGAGCGTGAACGTTATATCGGTGTCTCCGGCGTGTTGCTGAAATCCGATCTCTACCTGACGCTGGGGATCTCCGGGCAGATCCAGCATATGGTTGGCGGCAGCGGCGCAAAAGTGAt                                                                                                                       >  2:1405797/1‑140 (MQ=255)          aGGCGAGAACTGGATGGAGCGTGAACGTTATATCGGTGTCTCCGGCGTGTTGCTGAAATCCGATCTCTACCTGACGCTGGGGATCTCCGGGCAGATCCAGCATATGGTTGGCGGCAGCGGCGCAAAAGTGATTGTCGcc                                                                                                                >  1:1333708/1‑139 (MQ=255)          aGGCGAGAACTGGATGGAGCGTGAACGTTATATCGGTGTCTCCGGCGTGTTGCTGAAATCCGATCTCTACCTGACGCTGGGGATCTCCGGGCAGATCCAGCATATGGTTGGCGGCAGCGGCGCAAAAGTGATTGTCGcc                                                                                                                <  2:1333708/139‑1 (MQ=255)          aGGCGAGAACTGGATGGAGCGTGAACGTTATATCGGTGTCTCCGGCGTGTTGCTGAAATCCGATCTCTACCTGACGCTGGGGATCTCCGGGCAGATCCAGCATATGGTTGGCGGCAGCGGCGCAAAAGTGATTGTCGCCa                                                                                                               <  2:1313077/140‑1 (MQ=255)                               tGAACGTTATATCGGTGTCTCCGGCGTGTTGCTGAAATCCGATCTCTACCTGACGCTGGGGATCTCCGGGCAGATCCAGCATATGGTTGGCGGCAGCGGCGCAAAAGTGATTGTCGCCATCAATAAAGATAAAAATgcgc                                                                                          <  2:657105/140‑1 (MQ=255)                               tGAACGTTATATCGGTGTCTCCGGCGTGTTGCTGAAATCCGATCTCTACCTGACGCTGGGGATCTCCGGGCAGATCCAGCATATGGTTGGCGGCAGCGGCGCAAAAGTGATTGTCGCCATCAATAAAGATAAAAATgcgc                                                                                          <  2:122390/140‑1 (MQ=255)                                gAACGTTATATCGGTGTCTCCGGCGTGTTGCTGAAATCCGATCTCTACCTGACGCTGGGGATCTCCGGGCAGATCCAGCATATGGTTGGCGGCAGCGGCGCAAAAGTGATTGTCGCCATCAATAAAGATAAAAATGCGcc                                                                                         >  2:176167/1‑140 (MQ=255)                                 aaCGTTATATCGGTGTCTCCGGCGTGTTGCTGAAATCCGATCTCTACCTGACGCTGGGGATCTCCGGGCAGATCCAGCATATGGTTGGCGGCAGCGGCGCAAAAGTGATTGTCGCCATCAATAAAGATAAAAATGCGCCa                                                                                        >  1:1388329/1‑140 (MQ=255)                                             gtgtCTCCGGCGTGTTGCTGAAATCCGATCTCTACCTGACGCTGGGGATCTCCGGGCAGATCCAGCATATGGTTGGCGGCAGCGGCGCAAAAGTGATTGTCGCCATCAATAAAGATAAAAATGCGCCAATCTTCAACTAt                                                                            >  2:83724/1‑140 (MQ=255)                                             gtgtCTCCGGCGTGTCGCTGAAATCCGATCTCTACCTGACGCTGGGGATCTCCGGGCAGATCCAGCATATGGTTGGCGGCAGCGGCGCAAAAGTGATTGTCGCCATCAATAAAGATAAAAATGCGCCAATCTTCAACTAt                                                                            >  1:67789/1‑140 (MQ=255)                                              tgtCTCCGGCGTGTTGCTGAAATCCGATCTCTACCTGACGCTGGGGATCTCCGGGCAGATCCAGCATATGGTTGGCGGCAGCGGCGCAAAAGTGATTGTCGCCATCAATAAAGATAAAAATGCGCCAATCTTCAACTATg                                                                           >  1:488613/1‑140 (MQ=255)                                              tgtCTCCGGCGTGTTGCTGAAATCCGATCTCTACCTGACGCTGGGGATCTCCGGGCAGATCCAGCATATGGTTGGCGGCAGCGGCGCAAAAGTGATTGTCGCCATCAATAAAGATAAAAATGCGCCAATCTTCAACTATg                                                                           >  2:465035/1‑140 (MQ=255)                                              tgtCTCCGGCGTGTTGCTGAAATCCGATCTCTACCTGACGCTGGGGATCTCCGGGCAGATCCAGCATATGGTTGGCGGCAGCGGCGCAAAAGTGATTGTCGCCATCAATAAAGATAAAAATGCGCCAATCTTCAACTATg                                                                           >  2:1002005/1‑140 (MQ=255)                                                tctcCGGCGTGTTGCTGAAATCCGATCTCTACCTGACGCTGGGGATCTCCGGGCAGATCCAGCATATGGTTGGCGGCAGCGGCGCAAAAGTGATTGTCGCCATCAATAAAGATAAAAATGCGCCAATCTTCAACTATGcc                                                                         >  2:507648/1‑140 (MQ=255)                                                tctcCGGCGTGTTGCTGAAATCCGATCTCTACCTGACGCTGGGGATCTCCGGGCAGATCCAGCATATGGTTGGCGGCAGCGGCGCAAAAGTGATTGTCGCCATCAATAAAGATAAAAATGCGCCAATCTTCAACTATGcc                                                                         <  1:1405797/140‑1 (MQ=255)                                                tctcCCGCGTGTTGCTAAAATCCGATCTCTACCTGCCGCTGGGGATCTCCGCGCAGATCCAGCATATGGTTAGCGCCCGCGGCGCAAAAATGATTGTCGCCATCAATAAAGATAAAAATGCGCCAATCTTCACCTATGcc                                                                         >  2:11319/1‑140 (MQ=25)                                                  tcCGGCGTGTTGCTGAAATCCGATCTCTACCTGACGCTGGGGATCTCCGGGCAGATCCAGCATATGGTTGGCGGCAGCGGCGCAAAAGTGATTGTCGCCATCAATAAAGATAAAAATGCGCCAATCTTCAACTATGCCGa                                                                       <  2:264764/140‑1 (MQ=255)                                                  tcCGGCGTGTTGCTGAAATCCGATCTCTACCTGACGCTGGGGATCTCCGGGCAGATCCAGCATATGGTTGGCGGCAGCGGCGCAAAAGTGATTGTCGCCATCAATAAAGATAAAAATGCGCCAATCTTCAACTATGCCGa                                                                       >  2:1322794/1‑140 (MQ=255)                                                         tgtTGCTGAAATCCGATCTCTACCTGACGCTGGGGATCTCCGGGCGGATCCAGCATATGGTTGGCGGCAGCGGCGCAAAAGTGATTGTCGCCATCAAGAAAGATGAAAATGCGCCAATCTTCAACTATGCCGACTACGGt                                                                >  2:971749/1‑140 (MQ=255)                                                          gtTGCTGAAATCCGATCTCTACCTGACGCTGGGGATCTCCGGGCAGATCCAGCATATGGTTGGCGGCAGCGGCGCAAAAGTGATTGTCGCCATCAATAAAGATAAAAATGCGCCAATCTTCAACTATGCCGACTACGGTc                                                               >  2:903087/1‑140 (MQ=255)                                                                   aTCCGATCTCTACCTGACGCTGGGGATCTCCGGGCAGATCCAGCATATGGTTGGCGGCAGCGGCGCAAAAGTGATTGTCGCCATCAATAAAGATAAAAATGCGCCAATCTTCAACTATGCCGACTACGGTCTGGTGGGCg                                                      <  2:661370/140‑1 (MQ=255)                                                                                cTGACGCTGGGGATCTCCGGGCAGATCCAGCATATGGTTGGCGGCAGCGGCGCAAAAGTGATTGTCGCCATCAATAAAGATAAAAATGCGCCAATCTTCAACTATGCCGACTACGGTCTGGTGGGCGATATCTACAAAgt                                         <  1:402815/140‑1 (MQ=255)                                                                                       tGGGGATCTCCGGGCAGATCCAGCATATGGTTGGCGGCAGCGGCGCAAAAGTGATTGTCGCCATCAATAAAGATAAAAATGCGCCAATCTTCAACTATGCCGACTACGGTCTGGTGGGCGATATCTACAAAGTCGTccct                                  >  2:1047842/1‑140 (MQ=255)                                                                                         gggATCTCCGGGCAGATCCAGCATATGGTTGGCGGCAGCGGCGCAAAAGTGATTGTCGCCATCAATAAAGATAAAAATGCGCCAATCTTCAACTATGCCGACTACGGTCTGGTGGGCGATATCTACAAAGTCGTccctgc                                <  1:176167/140‑1 (MQ=255)                                                                                           gATCTCCGGGTAGATCCAGCATATGGTTGGCGGCAGCGGCGCAAAAGTGATTGTCGCCATCAATAAAGATAAAAATGCGCCAATCTTCAACTATGCCGACTACGGTCTGGTGGGCGATATCTACAAAGTCGTccctgccc                              <  2:1314431/140‑1 (MQ=255)                                                                                                 cGGGCAGATCCAGCATATGGTTGGCGGCAGCGGCGCAAAAGTGATTGTCGCCATCAATAAAGATAAAAATGCGCCAATCTTCAACTATGCCGACTACGGTCTGGTGGGCGATATCTACAAAGTCGTCCCTGCCCTGATTa                        <  1:507648/140‑1 (MQ=255)                                                                                                        aTCCAGCATATGGTTGGCGGCAGCGGCGCAAAAGTGATTGTCGCCATCAATAAAGATAAAAATGCGCCAATCTTCAACTATGCCGACTACGGTCTGGTGGGCGATATCTACAAAGTCGTCCCTGCCCTGATTAGCCAGtt                 <  1:999302/140‑1 (MQ=255)                                                                                                        aTCCAGCATATGGTTGGCGGCAGCGGCGCAAAAGTGATTGTCGCCATCAATAAAGATAAAAATGCGCCAATCTTCAACTATGCCGACTACGGTCTGGTGGGCGATATCTACAAAGTCGTCCCTGCCCTGATTAGCCAGtt                 >  2:35955/1‑140 (MQ=255)                                                                                                        aTCCAGCATATGGTTGGCGGCAGCGGCGCAAAAGTGATTGTCGCCATCAATAAAGATAAAAATGCGCCAATCTTCAACTATGCCGACTACGGTCTGGTGGGCGATATCTACAAAGTCGTCCCTGCCCTGATTAGCCAGtt                 >  1:511552/1‑140 (MQ=255)                                                                                                                tatGGTTGGCGGCAGCGGCGCAAAAGTGATTGTCGCCATCAATAAAGATAAAAATGCGCCAATCTTCAACTATGCCGACTACGGTCTGGTGGGCGATATCTACAAAGTCGTCCCTGCCCTGATTAGCCAGTTGAGCCGCt         <  1:83724/140‑1 (MQ=255)                                                                                                                  tGGTTGGCGGCAGCGGCGCAAAAGTGATTGTCGCCATCAATAAAGATAAAAATGCGCCAATCTTCAACTATGCCGACTACGGTCTGGTGGGCGATATCTACAAAGTCGTCCCTGCCCTGATTAGCCAGTTGAGCCGCTaa       >  1:1388373/1‑140 (MQ=255)                                                                                                                  tGGTTGGCGGCAGCGGCGCAAAAGTGATTGTCGCCATCAATAAAGATAAAAATGCGCCAATCTTCAACTATGCCGACTACGGTCTGGTGGGCGATATCTACAAAGTCGTCCCTGCCCTGATTAGCCAGTTGAGCCGCTaa       >  1:1021434/1‑140 (MQ=255)                                                                                                                   ggTTGGCGGCAGCGGCGCAAAAGTGATTGTCGCCATCAATAAAGATAAAAATGCGCCAATCTTCAACTATGCCGACTACGGTCTGGTGGGCGATATCTACAAAGTCGTCCCTGCCCTGATTAGCCAGTTGAg              <  2:416741/132‑1 (MQ=255)                                                                                                                   ggTTGGCGGCAGCGGCGCAAAAGTGATTGTCGCCATCAATAAAGATAAAAATGCGCCAATCTTCAACTATGCCGACTACGGTCTGGTGGGCGATATCTACAAAGTCGTCCCTGCCCTGATTAGCCAGTTGAg              >  1:416741/1‑132 (MQ=255)                                                                                                                   ggTTGGCGGCAGCGGCGCAAAAGTGATTGTCGCCATCAATAAAGATAAAAATGCGCCAATCTTCAACTATGCCGACTACGGTCTGGTGGGCGATATCTACAAAGTCGTCCCTGCCCTGATTAGCCAGTTGAGCCGCTAAt      >  2:1013427/1‑140 (MQ=255)                                                                                                                     ttGGCGGCAGCGGCGCAAAAGTGATTGTCGCCATCAATAAAGATAAAAATGCGCCAATCTTCAACTATGCCGACTACGGTCTGGTGGGCGATATCTACAAAGTCGTCCCTGCCCTGATTAGCCAGTTGAGCCGCTAATTc    >  2:253937/1‑140 (MQ=255)                                                                                                                      tGGGGGCAGCGGCGCAAAAGTGATTGTCGCCATCAATAAAGATAAAAATGCGCCAATCTTCAACTATGCCGACTACGGTCTGGTGGGCGATATCTACAAAGTCGTCCCTG‑CCTGATTAGCCAGTTGAGCCGCTAATTccc  >  2:521550/1‑140 (MQ=255)                                                                                                                       ggcggcAGCGGCGCAAAAGTGATTGTCGCCATCAATAAAGATAAAAATGCGCCAATCATCAACTATGCCGACTACGATCTGGTGGGCGATATCTACAAACTCGTCCCTGCCCTGATTAGCCAATTGAGCCGCTAATTccc  <  2:1388329/140‑1 (MQ=255)                                                                                                                        gcggcAGCGGCGCAAAAGTGATTGTCGCCATCAATAAAGATAAAAATGCGCCAATCTTCAACTATGCCGACTACGGTCTGGTGGGCGATATCTACAAAGTCGTCCCTGCCCTGATTAGCCAGTTGAGcc            <  2:297206/129‑1 (MQ=255)                                                                                                                        gcggcAGCGGCGCAAAAGTGATTGTCGCCATCAATAAAGATAAAAATGCGCCAATCTTCAACTATGCCGACTACGGTCTGGTGGGCGATATCTACAAAGTCGTCCCTGCCCTGATTAGCCAGTTGAGcc            >  1:297206/1‑129 (MQ=255)                                                                                                                              |                                                                                                                                     AATTGCCGAAGGCGAGAACTGGATGGAGCGTGAACGTTATATCGGTGTCTCCGGCGTGTTGCTGAAATCCGATCTCTACCTGACGCTGGGGATCTCCGGGCAGATCCAGCATATGGTTGGCGGCAACGGCGCAAAAGTGATTGTCGCCATCAATAAAGATAAAAATGCGCCAATCTTCAACTATGCCGACTACGGTCTGGTGGGCGATATCTACAAAGTCGTCCCTGCCCTGATTAGCCAGTTGAGCCGCTAATTCCC  >  NC_000913/1781087‑1781344

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑