Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,942,267	T→C	Q106R (CAG→CGG)	znuA ←	Zn(2(+)) ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,942,267	0	T	C	100.0%	160.2 / NA	46	Q106R (CAG→CGG)	znuA	Zn(2(+)) ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (25/21);  total (25/21)

CGCTATCTCTGGGGAAAGCCAAAGATGCATGTTGAAATCGCCGTGATGGTGATCTTCGTCACTTTTTTCCGCGTGGTCGTGATCATCATCATCGCCGTGAATACTTTTCATCAGTAGCGGTTTCACATCTTCAAGCTGCGCAATCGTTACCTGCTTCGCTCCTGGTAATTTGCTTACCGGTTTTTGCATAAACGCTTCCATCTCCGGGCCAACCCAAACGACTAAGTCCGCGTTCTGTAAGCGTTTTACATCCGATGGGCGCAGTGAATAATC  >  NC_000913/1942131‑1942403                                                                                                                                         |                                                                                                                                         cgcTATCTCTGGGGAAAGCCAAAGATGCATGTTGAAATCGCCGTGATGGTGATCTTCGTCACTTTTTTCCGCGTGGTCGTGATCATCATCATCGCCGTGAATACTTTTCATCAGTAGCGGTTTCACATCTTCAAGCcgcg                                                                                                                                       >  1:426889/1‑140 (MQ=255) cgcTATCTCTGGGGAAAGCCAAAGATGCATGTTGAAATCGCCGTGATGGTGATCTTCGTCACTTTTTGCCGCGTGGTCGTGATCATCATCATCGCCGTGAATACTTTTCATCAGTAGCGGTTTCACATCTTCAAGCcgcg                                                                                                                                       <  2:43663/140‑1 (MQ=255)           ggggAAAGCCAAAGATGCATGTTGAAATCGCCGTGATGGTGATCTTCGTCACTTTTTTCCGCGTGGTCGTGATCATCATCATCGCCGTGAATACTTTTCATCAGTAGCGGTTTCACATCTTCAAGCCGCGCAATCGTTAc                                                                                                                             <  1:440212/140‑1 (MQ=255)                 aGCCAAAGATGCATGTTGAAATCGCCGTGATGGTGATCTTCGTCACTTTTTTCCGCGTGGTCGTGATCATCATCATCGCCGTGAATACTTTTCATCAGTTGCGGTTTCACATCTTCAAGCCGCGCAATCGTTACCTGCtt                                                                                                                       >  1:592054/1‑140 (MQ=255)                 aGCCAAAGATGCATGTTGAAATCGCCGTGATGGTGATCTTCGTCACTTTTTTCCGCGTGGTCGTGATCATCATCATCGCCGTGAATACTTTTCATCAGTAGCGGTTTCACATCTTCAAGCCGCGCAATCGTTACCTGCtt                                                                                                                       >  1:207054/1‑140 (MQ=255)                      aaGATGCATGTTGAAATCGCCGTGATGGTGATCTTCGTCACTTTTTTCCGCGTGGTCGTGATCATCATCATCGCCGTGAATACTTTTCATCAGTAGCGGTTTCACATCTTCAAGCCGCGCAATCGTTACCTGCTTCGCTc                                                                                                                  >  2:702676/1‑140 (MQ=255)                            cATGTTGAAATCGCCGTGATGGTGATCTTCGTCACTTTTTTCCGCGTGGTCGTGATCATCATCATCGCCGTGAATACTTTTCATCAGTAGCGGTTTCACATCTTCAAGCCGCGCAATCGTTACCTGCTTCGCTCCTGGTa                                                                                                            >  2:1254756/1‑140 (MQ=255)                             aTGTTGAAATCGCCGTGATGGTGATCTTCGTCACTTTTTTCCGCGTGGTCGTGATCATCATCATCGCCGTGAATACTTTTCATCAGTAGCGGTTTCACATCTTCAAGCCGCGCAATCGTTACCTGCTTCGCTCCTGGTaa                                                                                                           >  2:315388/1‑140 (MQ=255)                                      tCGCCGTGATGGTGATCTTCGTCACTTTTTTCCGCGTGGTCGTGATCATCATCATCGCCGTGAATACTTTTCATCAGTAGCGGTTTCACATCTTCAAGCCGCGCAATCGTTACCTGCTTCGCTCCTGGTAATTTGCTTAc                                                                                                  <  2:552223/140‑1 (MQ=255)                                      tCGCCGTGATGGTGATCTTCGTCACTTTTTTCCGCGTGGTCGTGATCATCATCATCGCCGTGAATACTTTTCATCAGTAGCGGTTTCACATCTTCAAGCCGCGCAATCGTTACCTGCTTCGCTCCTGGTAATTTGCTTAc                                                                                                  <  1:1122036/140‑1 (MQ=255)                                                      cTTCGTCACTTTTTTCCGCGTGGTCGTGATCATCATCATCGCCGTGAATACTTTTCATCAGTAGCGGTTTCACATCTTCAAGCCGCGCAATCGTTACCTGCTTCGCTCCTGGTAATTTGCTTACCGGTTTTTGCATAAAc                                                                                  <  2:349471/140‑1 (MQ=255)                                                          gTCACTTTTTTCCGCGTGGTCGTGATCATCATCATCGCCGTGAATACTTTTCATCAGTAGCGGTTTCACATCTTCAAGCCGCGCAATCGTTACCTGCTTCGCTCCTGGTAATTTGCTTACCGGTTTTTGCATAAACGCtt                                                                              >  2:604191/1‑140 (MQ=255)                                                                   ttCCGCGTGGTCGTGATCATCATCATCGCCGTGAATACTTTTCATCAGTAGCGGTTTCACATCTTCAAGCCGCGCAATCGTTACCTGCTTCGCTCCTGGTAATTTGCTTACCGGTTTTTGCATAAACGCTTCCATCTCCg                                                                     >  1:632474/1‑140 (MQ=255)                                                                      cgcgTGGTCGTGATCATCATCATCGCCGTGAATACTTTTCATCAGTAGCGGTTTCACATCTTCAAGCCGCGCAATCGTTACCTGCTTCGCTCCTGGTAATTTGCTTACCGGTTTTTGCATAAACGCTTCCATCTCCGGGc                                                                  <  2:739961/140‑1 (MQ=255)                                                                        cgTGGTCGTGATCATCATCATCGCCGTGAATACTTTTCATCAGTAGCGGTTTCACATCTTCAAGCCGCGCAATCGTTACCTGCTTCGCTCCTGGTAATTTGCTTACCGGTTTTTGCATAAACGCTTCCATCTCCGGGCCa                                                                <  2:632474/140‑1 (MQ=255)                                                                         gTGGTCGTGATCATCATCATCGCCGTGAATACTTTTCATCAGTAGCGGTTTCACATCTTCAAGCCGCGCAATCGTTACCTGCTTCGCTCCTGGTAATTTGCTTACCGGTTTTTGCATAAACGCTTCCATCTCCGGGCCaa                                                               >  1:1385188/1‑140 (MQ=255)                                                                         gTGGTCGTGATCATCATCATCGCCGTGAATACTTTTCATCAGTAGCGGTTTCACATCTTCAAGCCGCGCAATCGTTACCTGCTTCGCTCCTGGTAATTTGCTTACCGGTTTTTGCATAAACGCTTCCATCTCCGGGCCaa                                                               >  1:190083/1‑140 (MQ=255)                                                                              cGTGATCATCATCATCGCCGTGAATACTTTTCATCAGTAGCGGTTTCACATCTTCAAGCCGCGCAATCGTTACCTGCTTCGCTCCTGGTAATTTGCTTACCGGTTTTTGCATAAACGCTTCCATCTCCGGGCCAACCCaa                                                          <  2:207054/140‑1 (MQ=255)                                                                                       catcatcGCCGTGAATACTTTTCATCAGTAGCGGTTTCACATCTTCAAGCCGCGCAATCGTTACCTGCTTCGCTCCTGGTAATTTGCTTACCGGTTTTTGCATAAACGCTTCCATCTCCGGGCCAACCCAAACGACTAAg                                                 >  2:594220/1‑140 (MQ=255)                                                                                       catcatcGCCGTGAATACTTTTCATCAGTAGCGGTTTCACATCTTCAAGCCGCGCAATCGTTACCTGCTTCGCTCCTGGTAATTTGCTTACCGGTTTTTGCATAAACGCTTCCATCTCCGGGCCAACCCAAACGACTAAg                                                 >  2:700112/1‑140 (MQ=255)                                                                                       catcatcGCCGTGAATACTTTTCATCAGTAGCGGTTTCACATCTTCAAGCCGCGCAATCGTTACCTGCTTCGCTCCTGGTAATTTGCTTACCGGTTTTTGCATAAACGCTTCCATCTCCGGGCCAACCCAAACGACTAAg                                                 >  2:526605/1‑140 (MQ=255)                                                                                       catcatcGCCGTGAATACTTTTCATCAGTAGCGGTTTCACATCTTCAAGCCGCGCAATCGTTACCTGCTTCGCTCCTGGTAATTTGCTTACCGGTTTTTGCATAAACGCTTCCATCTCCGGGCCAACCCAAACGACTAAg                                                 >  1:726623/1‑140 (MQ=255)                                                                                       catcatcGCCGTGAATACTTTTCATCAGTAGCGGTTTCACATCTTCAAGCCGCGCAATCGTTACCTGCTTCGCTCCTGGTAATTTGCTTACCGGTTTTTGCATAAACGCTTCCATCTCCGGGCCAACCCAAACGACTAAg                                                 >  1:1287735/1‑140 (MQ=255)                                                                                       catcatcGCCGTGAATACTTTTCATCAGTAGCGGTTTCACATCTTCAAGCCGCGCAATCGTTACCTGCTTCGCTCCTGGTAATTTGCTTACCGGTTTTTGCATAAACGCTTCCATCTCCGGGCCAACCCAAACGACTAAg                                                 <  2:726623/140‑1 (MQ=255)                                                                                       catcatcGCCGTGAATACTTTTCATCAGTAGCGGTTTCACATCTTCAAGCCGCGCAATCGTTACCTGCTTCGCTCCTGGTAATTTGCTTACCGGTTTTTGCATAAACGCTTCCATCTCCGGGCCAACCCAAACGACTAAg                                                 >  2:308738/1‑140 (MQ=255)                                                                                           atcGCCGTGAATACTTTTCATCAGTAGCGGTTTCACATCTTCAAGCCGCGCAATCGTTACCTGCTTCGCTCCTGGTAATTTGCTTACCGGTTTTTGCATAAACGCTTCCATCTCCGGGCCAACCCAAACGACTa                                                   >  1:1164267/1‑134 (MQ=255)                                                                                           atcGCCGTGAATACTTTTCATCAGTAGCGGTTTCACATCTTCAAGCCGCGCAATCGTTACCTGCTTCGCTCCTGGTAATTTGCTTACCGGTTTTTGCATAAACGCTTCCATCTCCGGGCCAACCCAAACGACTa                                                   <  2:1164267/134‑1 (MQ=255)                                                                                           atcGCCGTGAATACTTTTCATCAGTAGCGGTTTCACATCTTCAAGCCGCGCAATCGTTACCTGCTTCGCTCCTGGTAATTTGCTTACCGGTTTTTGCATAAACGCTTCCATCTCCGGGCCAACCCAAACGACTAAGTCcg                                             >  2:939392/1‑140 (MQ=255)                                                                                              gCCGTGAATACTTTTCATCAGTAGCGGTTTCACATCTTCAAGCCGCGCAATCGTTACCTGCTTCGCTCCTGGTAATTTGCTTACCGGTTTTTGCATAAACGCTTCCATCTCCGGGCCAACCCAAACGACTAAGTCCGCGt                                          >  2:1103603/1‑140 (MQ=255)                                                                                                  tGAATACTTTTCATCAGTAGCGGTTTCACATCTTCAAGCCGCGCAATCGTTACCTGCTTCGCTCCTGGTAATTTGCTTACCGGTTTTTGCATAAACGCTTCCATCTCCGGGCCAACCCAAACGACTa                                                   >  2:369751/1‑127 (MQ=255)                                                                                                  tGAATACTTTTCATCAGTAGCGGTTTCACATCTTCAAGCCGCGCAATCGTTACCTGCTTCGCTCCTGGTAATTTGCTTACCGGTTTTTGCATAAACGCTTCCATCTCCGGGCCAACCCAAACGACTa                                                   <  1:369751/127‑1 (MQ=255)                                                                                                   gAATACTTTTCATCAGTAGCGGTTTCACATCTTCAAGCCGCGCAATCGTTACCTGCTTCGCTCCTGGTAATTTGCTTACCGGTTTTTGCATAAACGCTTCCATCTCCGGGCCAACCCAAACGACTAAGTCCGCGTTCTGt                                     <  1:1202095/140‑1 (MQ=255)                                                                                                         ttttCATCAGTAGCGGTTTCACATCTTCAAGCCGCGCAATCGTTACCTGCTTCGCTCCTGGTAATTTGCTTACCGGTTTTTGCATAAACGCTTCCATCTCCGGGCCAACCCAAACGACTAAGTCCGCGTTCTGTAAGCGt                               <  1:1254756/140‑1 (MQ=255)                                                                                                              atcaGTAGCGGTTTCACATCTTCAAGCCGCGCAATCGTTACCTGCTTCGCTCCTGGTAATTTGCTTACCGGTTTTTGCATAAACGCTTCCATCTCCGGGCCAACCCAAACGACTAAGTCCGCGTTCTGTAAGCGTTTTAc                          >  2:913014/1‑140 (MQ=255)                                                                                                              atcaGTAGCGGTTTCACATCTTCAAGCCGCGCAATCGTTACCTGCTTCGCTCCTGGTAATTTGCTTACCGGTTTTTGCATAAACGCTTCCATCTCCGGGCCAACCCAAACGACTAAGTCCGCGTTCTGTAAGCGTTTTAc                          >  1:213274/1‑140 (MQ=255)                                                                                                                  gTAGCGGTTTCACATCTTCAAGCCGCGCAATCGTTACCTGCTTCGCTCCTGGTAATTTGCTTACCGGTTTTTGCATAAACGCTTCCATCTCCGGGCCAACCCAAACGACTAAGTCCGCGTTCTGTAAGCGTTTTACATcc                      <  1:594220/140‑1 (MQ=255)                                                                                                                   tAGCGGTTTCACATCTTCAAGCCGCGCAATCGTTACCTGCTTCGCTCCTGGTAATTTGCTTACCGGTTTTTGCATAAACGCTTCCATCTCCGGGCCAACCCAAACGACTAAGTCCGCGTTCTGTAAGCGTTTTACATCCg                     >  2:58802/1‑140 (MQ=255)                                                                                                                    aGCGGTTTCACATCTTCAAGCCGCGCAATCGTTACCTGCTTCGCTCCTGGTAATTTGCTTACCGGTTTTTGCATAAACGCTTCCATCTCCGGGCCAACCCAAACGACTAAGTCCGCGTTCTGTAAGCGTTTTACATCCGa                    <  1:702676/140‑1 (MQ=255)                                                                                                                     gCGGTTTCACATCTTCAAGCCGCGCAATCGTTACCTGCTTCGCTCCTGGTAATTTGCTTACCGGTTTTTGCATAAACGCTTCCATCTCCGGGCCAACCCAAACGACTAAGTCCGCGTTCTGTAAGCGTTTTACATCCGAt                   >  1:1089742/1‑140 (MQ=255)                                                                                                                        gTTTCACATCTTCAAGCCGCGCAATCGTTACCTGCTTCGCTCCTGGTAATTTGCTTACCGGTTTTTGCATAAACGCTTCCATCTCCGGGCCAACCCAAACGACTAAGTCCGCGTTCTGTAAGCGTTTTACATCCGATggg                >  1:437216/1‑140 (MQ=255)                                                                                                                          ttCACATCTTCAAGCCGCGCAATCGTTACCTGCTTCGCTCCTGGTAATTTGCTTACCGGTTTTTGCATAAACGCTTCCATCTCCGGGCCAACCCAAACGACTAAGTCCGCGTTCTGTAAGCGTTTTACATCCGATGGgcg              <  1:939392/140‑1 (MQ=255)                                                                                                                          ttCACATCTTCAAGCCGCGCAATCGTTACCTGCTTCGCTCCTGGTAATTTGCTTACCGGTTTTTGCATAAACGCTTCCATCTCCGGGCCAACCCAAACGACTAAGTCCGCGTTCTGTAAGCGTTTTACATCCGATGGgcg              <  1:679561/140‑1 (MQ=255)                                                                                                                           tCACATCTTCAAGCCGCGCAATCGTTACCTGCTTCGCTCCTGGTAATTTGCTTACCGGTTTTTGCATAAACGCTTCCATCTCCGGGCCAACCCAAACGACTAAGTCCGCGTTCTGTAAGCGTTTTACATCCGATGGgcgc             <  2:592054/140‑1 (MQ=255)                                                                                                                            cacaTCTTCAAGCCGCGCAATCGTTACCTGCTTCGCTCCTGGTAATTTGCTTACCGGTTTTTGCATAAACGCTTCCATCTCCGGGCCAACCCAAACGACTAAGTCCGCGTTCTGTAAGCGTTTTACATCCGATGGGCGCa            <  1:58802/140‑1 (MQ=255)                                                                                                                            cacaTCTTCAAGCCGCGCAATCGTTACCTGCTTCGCTCCTGGTAATTTGCTTACCGGTTTTTGCATAAACGCTTCCATCTCCGGGCCAACCCAAACGACTAAGTCCGCGTTCTGTAAGCGTTTTACATCCGATGGGCGCa            <  2:1287735/140‑1 (MQ=255)                                                                                                                            cacaTCTTCAAGCCGCGCAATCGTTACCTGCTTCGCTCCTGGTAATTTGCTTACCGGTTTTTGCATAAACGCTTCCATCTCCGGGCCAACCCAAACGACTAAGTCCGCGTTCTGTAAGCGTTTTACATCCGATGGGCGCa            <  1:913014/140‑1 (MQ=255)                                                                                                                               aTCTTCAAGCCGCGCAATCGTTACCTGCTTCGCTCCTGGTAATTTGCTTACCGGTTTTTGCATAAACGCTTCCATCTCCGGGCCAACCCAAACGACTAAGTCCGCGTTCTGTAAGCGTTTTACATCCGATGGGCGCAGTg         >  2:45251/1‑140 (MQ=255)                                                                                                                                      aGCCGCGCAATCGTTACCTGCTTCGCTCCTGGTAATTTGCTTACCGGTTTTTGCATAAACGCTTCCATCTCCGGGCCAACCCAAACGACTAAGTCCGCGTTCTGTAAGCGTTTTACATCCGATGGGCGCAGTGAATAATc  <  1:1103603/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                         CGCTATCTCTGGGGAAAGCCAAAGATGCATGTTGAAATCGCCGTGATGGTGATCTTCGTCACTTTTTTCCGCGTGGTCGTGATCATCATCATCGCCGTGAATACTTTTCATCAGTAGCGGTTTCACATCTTCAAGCTGCGCAATCGTTACCTGCTTCGCTCCTGGTAATTTGCTTACCGGTTTTTGCATAAACGCTTCCATCTCCGGGCCAACCCAAACGACTAAGTCCGCGTTCTGTAAGCGTTTTACATCCGATGGGCGCAGTGAATAATC  >  NC_000913/1942131‑1942403

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑