Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	217,605	C→T	V391M (GTG→ATG)	proS ←	proline‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	217,605	0	C	T	100.0%	245.6 / NA	70	V391M (GTG→ATG)	proS	proline‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (33/37);  total (33/37)

CATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATCACCAC  >  NC_000913/217476‑217737                                                                                                                                  |                                                                                                                                     cATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCATCACGTTACgg                                                                                                                            <  1:836207/140‑1 (MQ=255)         cccTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCATCCCGTTACGGATATCTGc                                                                                                                    <  2:762444/140‑1 (MQ=255)         cccTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCATCACGTTACGGATATCTGc                                                                                                                    <  1:130643/140‑1 (MQ=255)          ccTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCATCACGTTACGGATATCTGCa                                                                                                                   <  1:370052/140‑1 (MQ=255)              tACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCATCACGTTACGGATATCTGCAACtt                                                                                                               >  2:382004/1‑140 (MQ=255)              tACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCATCACGTTACGGATATCTGCAACtt                                                                                                               >  1:876493/1‑140 (MQ=255)              tACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCATCACGTTACGGATATCTGCAACtt                                                                                                               >  2:716437/1‑140 (MQ=255)              tACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCATCACGTTACGGATATCTGCAACtt                                                                                                               >  1:425688/1‑140 (MQ=255)               aCGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCATCACGTTACGGATATCTGCAACTTc                                                                                                              <  1:340637/140‑1 (MQ=255)                   aGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCATCACGTTACGGATATCTGCa                                                                                                                   >  1:1127537/1‑131 (MQ=255)                   aGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCATCACGTTACGGATATCTGCa                                                                                                                   <  2:1127537/131‑1 (MQ=255)                    ggCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCATCACGTTACGGATATCTGCAACTTCCgggg                                                                                                         >  2:268922/1‑140 (MQ=255)                        ttCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCATCACGTTACGGATATCTGCAACTTCCGGGGTAGc                                                                                                     >  2:604954/1‑140 (MQ=255)                        ttCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCATCACGTTACGGATATCTGCAACTTCCGGGGTAGc                                                                                                     >  1:229384/1‑140 (MQ=255)                        ttCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCATCACGTTACGGATATCTGCAACTTCCGGGGTAGc                                                                                                     >  2:803570/1‑140 (MQ=255)                        ttCAGTGCTTCGGAGTACTTGGTACCCAGCT‑GAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCATCACGTTACGGATATCTGCAACTTCCGGGGTAGCg                                                                                                    >  1:485139/1‑140 (MQ=255)                           aGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCATCACGTTACGGATATCTGCAACTTCCGGGGTAGCGAc                                                                                                  <  1:803570/140‑1 (MQ=255)                               cTTCGGAGTACTTGGTACCCAGCT‑GAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCATCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATcgc                                                                                             <  2:485139/140‑1 (MQ=255)                                ttCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCATCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATcgc                                                                                             <  1:604954/140‑1 (MQ=255)                                  cGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCATCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGa                                                                                           >  1:1022986/1‑140 (MQ=255)                                         cTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTAATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCATCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGt                                                                                    <  2:1022986/140‑1 (MQ=255)                                          ttGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCATCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGtt                                                                                   <  1:108184/140‑1 (MQ=255)                                            ggTACCCAGCTGGGAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCATCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGa                                                                                 >  2:1301412/1‑140 (MQ=255)                                              tACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCATCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATg                                                                               >  2:847157/1‑140 (MQ=255)                                              tACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCATCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATg                                                                               <  2:876493/140‑1 (MQ=255)                                               aCCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCATCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGc                                                                              <  1:847157/140‑1 (MQ=255)                                               aCCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCATCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGc                                                                              >  1:974531/1‑140 (MQ=255)                                                 ccAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCATCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCg                                                                            >  1:1389097/1‑140 (MQ=255)                                                   aGCTGGGAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCATCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGaa                                                                          <  1:1301412/140‑1 (MQ=255)                                                   aGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCATCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGaa                                                                          >  2:449796/1‑140 (MQ=255)                                                   aGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCATCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGaa                                                                          >  1:434896/1‑140 (MQ=255)                                                    gCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCATCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAag                                                                         <  1:449796/140‑1 (MQ=255)                                                     cTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCATCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAagt                                                                        >  1:756244/1‑140 (MQ=255)                                                      tGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCATCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAagta                                                                       <  1:221615/140‑1 (MQ=255)                                                      tGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCATCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAagta                                                                       <  1:184299/140‑1 (MQ=255)                                                          aGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCATCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAgtgt                                                                   >  1:181229/1‑140 (MQ=255)                                                                gACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCATCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCa                                                             <  2:229384/140‑1 (MQ=255)                                                                 aCCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCATCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCAt                                                            <  1:1210131/140‑1 (MQ=255)                                                                 aCCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCATCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCAt                                                            <  2:756244/140‑1 (MQ=255)                                                                  ccAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCATCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATc                                                           <  2:181229/140‑1 (MQ=255)                                                                  ccAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCATCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATc                                                           <  2:408461/140‑1 (MQ=255)                                                                  ccAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCATCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATc                                                           <  1:309775/140‑1 (MQ=255)                                                                  ccAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCATCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATc                                                           <  2:425688/140‑1 (MQ=255)                                                                  ccAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCATCACGTTACGGATATCTGCAACTTCCGGGGCAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATc                                                           <  2:580414/140‑1 (MQ=255)                                                                   cAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCATCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCg                                                          <  2:974531/140‑1 (MQ=255)                                                                         cGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCATCACTTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTa                                                    >  2:1115394/1‑140 (MQ=255)                                                                           aTACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCATCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGc                                                  >  1:1070879/1‑140 (MQ=255)                                                                              ccACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCATCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCAcc                                               <  2:1231024/140‑1 (MQ=255)                                                                              ccACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCATCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCAcc                                               <  1:446466/140‑1 (MQ=255)                                                                               cACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCATCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACca                                              <  2:434896/140‑1 (MQ=255)                                                                                 cGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCATCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACcagc                                            >  2:375560/1‑140 (MQ=255)                                                                                     tGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCATCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCg                                        >  2:233592/1‑140 (MQ=255)                                                                                     tGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCATCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCg                                        <  2:1070879/140‑1 (MQ=255)                                                                                         cagcagCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCATCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAAt                                    >  2:580615/1‑140 (MQ=255)                                                                                            cagcCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCATCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCAc                                 >  2:387850/1‑140 (MQ=255)                                                                                                  aCCCTGGCCATCCGGGCTTGGATCGCCAGCCATCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATcg                           <  2:1389097/140‑1 (MQ=255)                                                                                                   cccTGGCCATCCGGGCTTGGATCGCCAGCCATCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATcgc                          <  1:233592/140‑1 (MQ=255)                                                                                                   cccTGGCCATCCGGGCTTGGATCGCCAGCCATCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATcgc                          >  1:631903/1‑140 (MQ=255)                                                                                                       ggCCATCCGGGCTTGGATCGCCAGCCATCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCa                      <  1:382004/140‑1 (MQ=255)                                                                                                        gCCATCCGGGCTTGGATCGCCAGCCATCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCaa                     >  1:809489/1‑140 (MQ=255)                                                                                                         ccATCCGGGCTTGGATCGCCAGCCATCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCGGCAGCGAAATCACTCATCGCCGCAac                    >  1:844775/1‑140 (MQ=255)                                                                                                         ccATCCGGGCTTGGATCGCCAGCCATCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAac                    >  1:267128/1‑140 (MQ=255)                                                                                                          cATCCGGGCTTGGATCGCCAGCCATCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAacg                   <  2:341660/140‑1 (MQ=255)                                                                                                             ccGGGCTTGGATCGCCAGCCATCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAacggta                >  2:124999/1‑140 (MQ=255)                                                                                                                 gCTTGGATCGCCAGCCATCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAacggtacggt            <  2:809489/140‑1 (MQ=255)                                                                                                                     ggATCGCCAGCCATCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAAt        <  2:715860/140‑1 (MQ=255)                                                                                                                     ggATCGCCAGCCATCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAAt        <  2:1068434/140‑1 (MQ=255)                                                                                                                      gATCGCCAGCCATCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATc       >  2:859311/1‑140 (MQ=255)                                                                                                                      gATCGCCAGCCATCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATc       >  2:929786/1‑140 (MQ=255)                                                                                                                       aTCGCCAGCCATCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATca      >  1:1279546/1‑140 (MQ=255)                                                                                                                           ccagccaTCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATcaccac  <  2:631903/140‑1 (MQ=255)                                                                                                                                  |                                                                                                                                     CATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATCACCAC  >  NC_000913/217476‑217737

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑