Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,130,687	T→C	G64G (GGA→GGG)	wcaD ←	putative colanic acid polymerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,130,687	0	T	C	100.0%	311.4 / NA	89	G64G (GGA→GGG)	wcaD	putative colanic acid polymerase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (48/41);  total (48/41)

TGTGGCGAAATTGTTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGTCCCGCGCCAATCCCTAAGGCAATCATCAATTTTTTAACGCTGATGCGTTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCA  >  NC_000913/2130553‑2130823                                                                                                                                       |                                                                                                                                         tgtgGCGAAATTGTTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGCCCcgc                                                                                                                                     >  2:315451/1‑140 (MQ=255)     gCGAAATTGTTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGCCCCGCGCCa                                                                                                                                 <  2:118927/140‑1 (MQ=255)     gCGAAATTGTTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGCCCCGCGCCa                                                                                                                                 >  1:118927/1‑140 (MQ=255)     gCGAAATTGTTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGCCCCGCGCCa                                                                                                                                 <  1:777860/140‑1 (MQ=255)     gCGAAATTGTTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGCCCCGCGCCa                                                                                                                                 <  1:490592/140‑1 (MQ=255)       gAAATTGTTTTAAAACGAATACTCCACACCATGCCAATAATTATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGCCCCGCGCCAAt                                                                                                                               >  1:1217321/1‑140 (MQ=255)       gAAATTGTTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGCCCCGCGCCAAt                                                                                                                               <  2:170876/140‑1 (MQ=255)         aaTTGTTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGCCCCGCGCCAATcc                                                                                                                             <  2:915040/140‑1 (MQ=255)                        aaTACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGCCCCGCGCCAATCCCTAAGGCAAtcatca                                                                                                              >  1:284555/1‑140 (MQ=255)                         aTACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTCGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGCCCCGCGCCAATCCCTAAGGCAATCATCaa                                                                                                             <  2:1262679/140‑1 (MQ=255)                              ccACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGCCCCGCGCCAATCCCTAAGGCAATCATCAAttttt                                                                                                        <  2:329674/140‑1 (MQ=255)                                 cacCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGCCCCGCGCCAATCCCTAAGGCAATCATCAATTTTTTaa                                                                                                     >  1:1294215/1‑140 (MQ=255)                                     aTGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGCCCCGCGCCAATCCCTAAGGCAATCATCAATTTTTTAACGCt                                                                                                 >  2:482546/1‑140 (MQ=255)                                      tGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGCCCCGCGCCAATCCCTAAGGCAATCATCAATTTTTTAACGCTg                                                                                                >  1:386485/1‑140 (MQ=255)                                      tGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGCCCCGCGCCAATCCCTAAGGCAATCATCAATTTTTTAACGCTg                                                                                                >  1:1272763/1‑140 (MQ=255)                                      tGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGCCCCGCGCCAATCCCTAAGGCAATCATCAATTTTTTAACGCTg                                                                                                >  2:752962/1‑140 (MQ=255)                                      tGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGCCCCGCGCCAATCCCTAAGGCAAGCATCAATTTTTTAACGCTg                                                                                                >  1:1018243/1‑140 (MQ=255)                                       gCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGCCCCGCGCCAATCCCTAAGGCAATCATCAATTTTTTAACGCTGa                                                                                               >  1:253839/1‑140 (MQ=255)                                       gCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGCCCCGCGCCAATCCCTAAGGCAATCATCAATTTTTTAACGCTGa                                                                                               >  2:351902/1‑140 (MQ=255)                                       gCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGCCCCGCGCCAATCCCTAAGGCAATCATCAATTTTTTAACGCTGa                                                                                               >  1:707801/1‑140 (MQ=255)                                       gCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGCCCCGCGCCAATCCCTAAGGCAATCATCAATTTTTTAACGCTGa                                                                                               >  2:401270/1‑140 (MQ=255)                                       gCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGCCCCGCGCCAATCCCTAAGGCAATCATCAATTTTTTAACGCTGa                                                                                               >  2:855749/1‑140 (MQ=255)                                       gCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGCCCCGCGCCAATCCCTAAGGCAATCATCAATTTTTTAACGCTGa                                                                                               >  1:472353/1‑140 (MQ=255)                                       gCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTCGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGCCCCGCGCCAATCCCTAAGGCAATCATCAATTTTTTAACGCTGa                                                                                               >  1:489735/1‑140 (MQ=255)                                        ccAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGCCCCGCGCCAATCCCTAAGGCAATCATCAATTTTTTAACGCTGAt                                                                                              <  1:1156407/140‑1 (MQ=255)                                         cAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGCCCCGCGCCAATCCCTAAGGCAATCATCAATTTTTTAACGCTGATg                                                                                             >  1:409642/1‑140 (MQ=255)                                           ataatGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGCCCCGCGCCAATCCCTAAGGCAATCATCAATTTTTTAACGCTGATGCg                                                                                           <  1:401270/140‑1 (MQ=255)                                             aatGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGCCCCGCGCCAATCCCTAAGGCAATCATCAATTTTTTAACGCTGATGCGtt                                                                                         <  2:213476/140‑1 (MQ=255)                                             aatGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGCCCCGCGCCAATCCCTAAGGCAATCATCAATTTTTTAACGCTGATGCGtt                                                                                         <  2:1294215/140‑1 (MQ=255)                                               tGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGCCCCGCGCCAATCCCTAAGGCAATCATCAATTTTTTAACGCTGATGCGTTCt                                                                                       <  1:855749/140‑1 (MQ=255)                                                     caATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGCCCCGCGCCAATCCCTAAGGCAATCATCAATTTTTTAACGCTGATGCGTTCTAAAAAc                                                                                 <  1:752962/140‑1 (MQ=255)                                                      aaTATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGCCCCGCGCCAATCCCTAAGGCAATCATCAATTTTTTAACGCTGATGCGTTCTAAAAAca                                                                                <  2:472353/140‑1 (MQ=255)                                                      aaTATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGCCCCGCGCCAATCCCTAAGGCAATCATCAATTTTTTAACGCTGATGCGTTCTAAAAAca                                                                                <  2:386485/140‑1 (MQ=255)                                                       atatAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGCCCCGCGCCAATCCCTAAGGCAATCATCAATTTTTTAACGCTGATGCGTTCTAAAAAcag                                                                               >  2:376232/1‑140 (MQ=255)                                                        tataGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGCCCCGCGCCAATCCCTAAGGCAATCATCAATTTTTTAACGCTGATGCGTTCTAAAAAcagc                                                                              <  2:1217321/140‑1 (MQ=255)                                                        tataGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGCCCCGCGCCAATCCCTAAGGCAATCATCAATTTTTTAACGCTGATGCGTTCTAAAAAcagc                                                                              <  1:315451/140‑1 (MQ=255)                                                        tataGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGCCCCGCGCCAATCCCTAAGGCAATCATCAATTTTTTAACGCTGATGCGTTCTAAAAAcagc                                                                              <  2:1363247/140‑1 (MQ=255)                                                        tataGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGCCCCGCGCCAATCCCTAAGGCAATCATCAATTTTTTAACGCTGATGCGTTCTAAAAAcagc                                                                              <  1:486666/140‑1 (MQ=255)                                                           aGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGCCCCGCGCCAATCCCTAAGGCAATCATCAATTTTTTAACGCTGATGCGTTCTAAAAACAGCAGt                                                                           >  1:772194/1‑140 (MQ=255)                                                             aCAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGCCCCGCGCCAATCCCTAAGGCAATCATCAATTTTTTAACGCTGATGCGTTCTAAAAACAGCAGTaa                                                                         >  2:561881/1‑140 (MQ=255)                                                             aCAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGCCCCGCGCCAATCCCTAAGGCAATCATCAATTTTTTAACGCTGATGCGTTCTAAAAACAGCAGTaa                                                                         >  2:36988/1‑140 (MQ=255)                                                             aCAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGCCCCGCGCCAATCCCTAAGGCAATCATCAATTTTTTAACGCTGATGCGTTCTAAAAACAGCAGTaa                                                                         >  1:312940/1‑140 (MQ=255)                                                                aaCAGCATAGTTGAAGTGAGGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGCCCCGCGCCAATCCCTAAGGCAATCATCAATTTTTTAACGCTGATGCGTTCTAAAAACAGCAGTAACaa                                                                      >  2:1121881/1‑140 (MQ=255)                                                                aaCAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGCCCCGCGCCAATCCCTAAGGCAATCATCAATTTTTTAACGCTGATGCGTTCTAAAAACAGCAGTAACaa                                                                      >  2:562723/1‑140 (MQ=255)                                                                aaCAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGCCCCGCGCCAATCCCTAAGGCAATCATCAATTTTTTAACGCTGATGCGTTCTAAAAACAGCAGTAACaa                                                                      <  2:284555/140‑1 (MQ=255)                                                                 aCAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGCCCCGCGCCAATCCCTAAGGCAATCATCAATTTTTTAACGCTGATGCGTTCTAAAAACAGCAGTAACAAg                                                                     >  1:366496/1‑140 (MQ=255)                                                                  cAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGCCCCGCGCCAATCCCTAAGGCAATCATCAATTTTTTAACGCTGATGCGTTCTAAAAACAGCAGTAACAAGa                                                                    >  2:539991/1‑140 (MQ=255)                                                                  cAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGCCCCGCGCCAATCCCTAAGGCAATCATCAATTTTTTAACGCTGATGCGTTCTAAAAACAGCAGTAACAAGa                                                                    >  1:516751/1‑140 (MQ=255)                                                                   aGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGCCCCGCGCCAATCCCTAAGGCAATCATCAATTTTTTAACGCTGATGCGTTCTAAAAACAGCAGTAACAAGAc                                                                   <  2:246675/140‑1 (MQ=255)                                                                   aGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGCCCCGCGCCAATCCCTAAGGCAATCATCAATTTTTTAACGCTGATGCGTTCTAAAAACAGCAGTAACAAGAc                                                                   <  2:489735/140‑1 (MQ=255)                                                                   aGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGCCCCGCGCCAATCCCTAAGGCAATCATCAATTTTTTAACGCTGATGCGTTCTAAAAACAGCAGTAACAAGAc                                                                   <  1:274935/140‑1 (MQ=255)                                                                   aGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGCCCCGCGCCAATCCCTAAGGCAATCATCAATTTTTTAACGCTGATGCGTTCTAAAAACAGCAGTAACAAGAc                                                                   <  2:772194/140‑1 (MQ=255)                                                                   aGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGCCCCGCGCCAATCCCTAAGGCAATCATCAATTTTTTAACGCTGATGCGTTCTAAAAACAGCAGTAACAAGAc                                                                   <  2:1272763/140‑1 (MQ=255)                                                                       tAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGCCCCGCGCCAATCCCTAAGGCAATCATCAATTTTTTAACGCTGATGCGTTCTAAAAAcagc                                                                              >  1:1047357/1‑125 (MQ=255)                                                                       tAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGCCCCGCGCCAATCCCTAAGGCAATCATCAATTTTTTAACGCTGATGCGTTCTAAAAAcagc                                                                              <  2:1047357/125‑1 (MQ=255)                                                                          ttGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGCCCCGCGCCAATCCCTAAGGCAATCATCAATTTTTTAACGCTGATGCGTTCTAAAAACAGCAGTAACAAGACAGGTaaa                                                            >  1:869263/1‑140 (MQ=255)                                                                             aaGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGCCCCGCGCCAATCCCTAAGGCAATCATCAATTTTTTAACGCTGATGCGTTCTAAAAACAGCAGTAACa                                                                       >  2:628054/1‑126 (MQ=255)                                                                             aaGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGCCCCGCGCCAATCCCTAAGGCAATCATCAATTTTTTAACGCTGATGCGTTCTAAAAACAGCAGTAACa                                                                       <  1:628054/126‑1 (MQ=255)                                                                             aaGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGCCCCGCGCCAATCCCTAAGGCAATCATCAATTTTTTAACGCTGATGCGTTCTAAAAACAGCAGTAACAAGACAGGTaaaaaa                                                         >  1:1079062/1‑140 (MQ=255)                                                                             aaGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGCCCCGCGCCAATCCCTAAGGCAATCATCAATTTTTTAACGCTGATGCGTTCTAAAAACAGCAGTAACAAGACAGGTaaaaaa                                                         >  2:1271474/1‑140 (MQ=255)                                                                               gTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGCCCCGCGCCAATCCCTAAGGCAATCATCAATTTTTTAACGCTGATGCGTTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGt                                                       >  2:748808/1‑140 (MQ=255)                                                                               gTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGCCCCGCGCCAATCCCTAAGGCAATCATCAATTTTTTAACGCTGATGCGTTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGt                                                       >  1:819337/1‑140 (MQ=255)                                                                               gTGACGTATTTACTGGCATCCAGCGACTGACC‑AACAGATAGTTAAACGCCGTGAGCCCCGCGCCAATCCCTAAGGCAATCATCAATTTTTTAACGCTGATGCGTTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTg                                                      >  1:992928/1‑140 (MQ=255)                                                                                tGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGCCCCGCGCCAATCCCTAAGGCAATCATCAATTTTTTAACGCTGATGCGTTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTg                                                      <  2:409642/140‑1 (MQ=255)                                                                                   cGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGCCCCGCGCCAATCCCTAAGGCAATCATCAATTTTTTAACGCTGATGCGTTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACg                                                   >  2:662539/1‑140 (MQ=255)                                                                                    gTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGCCCCGCGCCAATCCCTAAGGCAATCATCAATTTTTTAACGCTGATGCGTTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGa                                                  >  1:1399326/1‑140 (MQ=255)                                                                                    gTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGCCCCGCGCCAATCCCTAAGGCAATCATCAATTTTTTAACGCTGATGCGTTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGa                                                  <  1:684016/140‑1 (MQ=255)                                                                                        ttACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGCCCCGCGCCAATCCCTAAGGCAATCATCAATTTTTTAACGCTGATGCGTTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGt                                              <  2:320839/140‑1 (MQ=255)                                                                                        ttACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGCCCCGCGCCAATCCCTAAGGCAATCATCAATTTTTTAACGCTGATGCGTTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGt                                              >  1:60160/1‑140 (MQ=255)                                                                                              gCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGCCCCGCGCCAATCCCTAAGGCAATCATCAATTTTTTAACGCTGATGCGTTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATggg                                        >  1:983434/1‑140 (MQ=255)                                                                                                   cAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGCCCCGCGCCAATCCCTAAGGCAATCATCAATTTTTTAACGCTGATGCGTTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAAc                                   <  2:253839/140‑1 (MQ=255)                                                                                                        aCTGACCAAACAGATAGTTAAACGCCGTGAGCCCCGCGCCAATCCCTAAGGCAATCATCAATTTTTTAACGCTGATGCGTTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCg                              <  2:797275/140‑1 (MQ=255)                                                                                                            aCCAAACAGATAGTTAAACGCCGTGAGCCCCGCGCCAATCCCTAAGGCAATCATCAATTTTTTAACGCTGATGCGTTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCaa                          >  1:1234220/1‑140 (MQ=255)                                                                                                            aCCAAACAGATAGTTAAACACCGTGAGCCCCGCGCCAATCCCTAAGGCAATCATCAATTTTTTAACGCTGATGCGTTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCaa                          >  2:1202526/1‑140 (MQ=255)                                                                                                                  cAGATAGTTAAACGCCGTGAGCCCCGCGCCAATCCCTAAGGCAATCATCAATTTTTTAACGCTGATGCGTTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTggg                    <  2:1234220/140‑1 (MQ=255)                                                                                                                      tAGTTAAACGCCGTGAGCCCCGCGCCAATCCCTAAGGCAATCATCAATTTTTTAACGCTGATGCGTTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGAt                >  1:98645/1‑140 (MQ=255)                                                                                                                       aGTTAAACGCCGTGAGCCCCGCGCCAATCCCTAAGGCAATCATCAATTTTTTAACGCTGATGCGTTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGcc                            <  2:1116011/127‑1 (MQ=255)                                                                                                                       aGTTAAACGCCGTGAGCCCCGCGCCAATCCCTAAGGCAATCATCAATTTTTTAACGCTGATGCGTTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGcc                            >  1:1116011/1‑127 (MQ=255)                                                                                                                        gTTAAACGCCGTGAGCCCCGCGCCAATCCCTAAGGCAATCATCAATTTTTTAACGCTGATGCGTTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGAttt              <  1:539991/140‑1 (MQ=255)                                                                                                                        gTTAAACGCCGTGAGCCCCGCGCCAATCCCTAAGGCAATCATCAATTTTTTAACGCTGATGCGTTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGAttt              >  2:76280/1‑140 (MQ=255)                                                                                                                        gTTAAACGCCGTGAGCCCCGCGCCAATCCCTAAGGCAATCATCAATTTTTTAACGCTGATGCGTTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGAttt              <  2:1216888/140‑1 (MQ=255)                                                                                                                             aCGCCGTGAGCCCCGCGCCAATCCCTAAGGCAATCATCAATTTTTTAACGCTGATGCGTTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACg         <  1:561881/140‑1 (MQ=255)                                                                                                                               gCCGTGAGCCCCGCGCCAATCCCTAAGGCAATCATCAATTTTTTAACGCTGATGCGTTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGtt       >  2:1296303/1‑140 (MQ=255)                                                                                                                                 cGTGAGCCCCGCGCCAATCCCTAAGGCAATCATCAATTTTTTAACGCTGATGCGTTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTaa     >  2:1083673/1‑140 (MQ=255)                                                                                                                                  gTGAGCCCCGCGCCAATCCCTAAGGCAATCATCAATTTTTTAACGCTGATGCGTTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAAc    <  2:312940/140‑1 (MQ=255)                                                                                                                                   tGAGCCCCGCGCCAATCCCTAAGGCAATCATCAATTTTTTAACGCTGATGCGTTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAAcc   >  2:481109/1‑140 (MQ=255)                                                                                                                                    gAGCCCCGCGCCAATCCCTAAGGCAATCATCAATTTTTTAACGCTGATGCGTTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACca  <  1:351902/140‑1 (MQ=255)                                                                                                                                    gAGCCCCGCGCCAATCCCTAAGGCAATCATCAATTTTTTAACGCTGATGCGTTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACca  <  1:1202526/140‑1 (MQ=255)                                                                                                                                    gAGCCCCGCGCCAATCCCTAAGGCAATCATCAATTTTTTAACGCTGATGCGTTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACca  <  2:846966/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                         TGTGGCGAAATTGTTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGTCCCGCGCCAATCCCTAAGGCAATCATCAATTTTTTAACGCTGATGCGTTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCA  >  NC_000913/2130553‑2130823

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑