Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,260,172	T→C	A413A (GCA→GCG)	fruA ←	fructose‑specific PTS multiphosphoryl transfer protein FruA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,260,172	0	T	C	100.0%	305.5 / NA	87	A413A (GCA→GCG)	fruA	fructose‑specific PTS multiphosphoryl transfer protein FruA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (35/52);  total (35/52)

CGCTTTGTCGAATTTGCGACGCGCCACCATTGTTGCCAGACCCATTGCCAGCGGTGGCACCATACCTGCCGCCATAATCGCCGCCATCGGGCCATAGGTTTGAGTACTCAGCAGACCCACACCGAATGCGTACGCTGCTTTGTTTACCGGACCGCCCATGTCAGTACACATCATGCCACCGAGGATCGCCCCCAGCAGAACCGCATTCGCAGTCCCCATGGTCTGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGA  >  NC_000913/2260037‑2260308                                                                                                                                        |                                                                                                                                         cgcTTTGTCGAATTTGCGACGCGCCACCATTGTTGCCAGACCCATTGCCAGCGGTGGCACCATACCTGCCGCCATAATCGCCGCCATCGGGCCATAGGTTTGAGTACTCAGCAGACCCACACCGAATGCGTACGCCGCtt                                                                                                                                      >  1:583171/1‑140 (MQ=255)  gcTTTGTCGAATTTGCGACGCGCCACCATTGTTGCCAGACCCATTGCCAGCGGTGGCACCATACCTGCCGCCATAATCGCCGCCATCGGGCCATAGGTTTGAGTACTCAGCAGACCCACACCGAATGCGTACGCCGCttt                                                                                                                                     <  2:1216626/140‑1 (MQ=255)   cTTTGTCGAATTTGCGACGCGCCACCATTGTTGCCAGACCCATTGCCAGCGGTGGCACCATACCTGCCGCCATAATCGCCGCCATCGGGCCATAGGTTTGAGTACTCAGCAGACCCACACCGAATGCGTAcgccgc                                                                                                                                        >  2:531329/1‑136 (MQ=255)            aTTTGTGACGCGCCACCATTGTTGCCAGACCCATTGCCAGCGGTGGCACAATACCTGCCGCCATAATCGCCGCCATCGGGCCATAGGTTTGAGTACTCAGCAGACCCACACCGAATGCGTACGCCGCTTTGTTTACCGGa                                                                                                                           <  1:1375263/140‑1 (MQ=255)            aTTTGCGACGCGCCACCATTGTTGCCAGACCCGTTGCCAGCGGTGGCACCATACCTGCCGCCATAATCGCCGCCATCGGGCCATAGGTTTGAGTACTCAGCAAACCCACACCGAATGCGTACGCCGCTTTGTTTACCGGa                                                                                                                           <  2:1247241/140‑1 (MQ=255)            aTTTGCGACGCGCCACCATTGTTGCCAGACCCATTGCCAGCGGTGGCACCATACCTGCCGCCATAATCGCCGCCATCGGGCCATAGGTTTGAGTACTCAGCAGACCCACACCGAATGCGTACGCCGCTTTGTTTACCGGa                                                                                                                           <  2:594841/140‑1 (MQ=255)            aTTTGCGACGCGCCACCATTGTTGCCAGACCCATTGCCAGCGGTGGCACCATACCTGCCGCCATAATCGCCGCCATCGGGCCATAGGTTTGAGTACTCAGCAGACCCACACCGAATGCGTACGCCGCTTTGTTTACCGGa                                                                                                                           <  1:964887/140‑1 (MQ=255)            aTTTGCGACGCGCCACCATTGTTGCCAGACCCATTGCCAGCGGTGGCACCATACCTGCCGCCATAATCGCCGCCATCGGGCCATAGGTTTGAGTACTCAGCAGACCCACACCGAATGCGTACGCCGCTTTGTTTACCGGa                                                                                                                           <  1:18083/140‑1 (MQ=255)            aTTTGCGACCCGCCACCATTGTGGCCAGACCCAGTGCCAGGGGGGCCACAGTACCTGCCGCCATAATCGCCGCCATCGGGCCATAGGTTTGAGTACTCAGCAGACCCACACCAAATGCGTACGCCGCTTTGTTTACCGGa                                                                                                                           <  2:712302/140‑1 (MQ=255)                gCGACGCGCCACCATTGTTGCCAGACCCATTGCCAGCGGTGGCACCATACCTGCCGCCATAATCGCCGCCATCGGGCCATAGGTTTGAGTACTCAGCAGACCCACACCGAATGCGTACGCCGCTTTGTTTACCGGa                                                                                                                           <  1:1389634/136‑1 (MQ=255)                gCGACGCGCCACCATTGTTGCCAGACCCATTGCCAGCGGTGGCACCATACCTGCCGCCATAATCGCCGCCATCGGGCCATAGGTTTGAGTACTCAGCAGACCCACACCGAATGCGTACGCCGCTTTGTTTACCGGa                                                                                                                           >  2:1389634/1‑136 (MQ=255)                 caaCGCGCCACCATTGTTGCCAGACCCTTTGCCGGCGGTGGCACCATACCTGCCGCCATAATCGCCGCCATCGGGCCATAGGTTTGAGTACTCAGCAGACCCACACCGAATGCGTACGCCGCTTTGTTTACCGGACCGcc                                                                                                                      <  2:1064547/138‑1 (MQ=255)                 cGACGCGCCACCATTGTTGCCAGACCCATTGCCAGCGGTGGCACCATCCCTGCCGCCATAATCGCCGCCATCGGGCCATAGGTTTGAGTACTCAGCAGACCCACACCGAATGCGTACGCCGCTTTGTTTACCGGACCGcc                                                                                                                      <  2:67532/140‑1 (MQ=255)                 cGACGCGCCACCATTGTTGCCAGACCCATTGCCAGCGGTGGCACCATACCTGCCGCCATAATCGCCGCCATCGGGCCATAGGTTTGAGTACTCAGCAGACCCACACCGAATGCGTACGCCGCTTTGTTTACCGGACCGcc                                                                                                                      <  1:374425/140‑1 (MQ=255)                 cGACGCGCCACCATTGTTGCCAGACCCATTGCCAGCGGTGGCACCATACCTGCCGCCATAATCGCCGCCATCGGGCCATAGGTTTGAGTACTCAGCAGACCCACACCGAATGCGTACGCCGCTTTGTTTACCGGACCGcc                                                                                                                      <  2:54716/140‑1 (MQ=255)                  gACGCGCCACCATTGTTGCCAGACCCATTGCCAGCGGTGGCACCATACCTGCCGCCATAATCGCCGCCATCGGGCCATAGGTTTGAGTACTCAGCAGACCCACACCGAATGCGTACGCCGCTTTGTTTACCGGACCGccc                                                                                                                     >  2:1185091/1‑140 (MQ=255)                  gACGCGCCACCATTGTTGCCAGACCCATCGCCAGCGGTGGCACCATACCTGCCGCCATAATCGCCGCCATCGGGCCATAGGTTTGAGTACTCAGCAGACCCACACCGAATGCGTACGCCGCTTTGTTTACCGGACCGccc                                                                                                                     >  2:311995/1‑140 (MQ=255)                   aCGCGCCACCATTGTTGCCAGACCCATTGCCAGCGGTGGCACCATCCCTGCCGCCATAATCGCCGCCATCGGGCCATAGGTTTGAGTACTCAGCAGACGCACACCGAATGCGTACGCCGCTTTGTTTACCGGACCGCCCa                                                                                                                    <  2:1226926/140‑1 (MQ=255)                    cgcgCCACCATTGTTGCCAGACCCATTGCCAGCGGTGGCACCATACCTGCCGCCATAATCGCCGCCATCGGGCCATAGGTTTGAGTACTCAGCAGACCCACACCGAATGCGTACGCCGCTTTGTTTACCGGACCGCCCAt                                                                                                                   <  1:1211482/140‑1 (MQ=255)                     gcgcCACCATTGTTGCCAGACCCATTGCCAGCGGTGGCACCATACCTGCCGCCATAATCGCCGCCATCGGGCCATAGGTTTGAGTACTCAGCAGACCCACACCGAATGCGTACGCCGCTTTGTTTACCGGACCGCCCATg                                                                                                                  >  1:1362765/1‑140 (MQ=255)                      cgcCACCATTGTTGCCAGACCCATTGCCAGCGGTGGCACCATACCTGCCGCCATAATCGCCGCCATCGGGCCATAGGTTTGAGTACTCAGCAGACCCACACCGAATGCGTACGCCGCTTTGTTTACCGGACCGCCCATGt                                                                                                                 <  1:1289436/140‑1 (MQ=255)                      cgcCACCATTGTTGCCAGACCCATTGCCAGCGGTGGCACCATACCTGCCGCCATAATCGCCGCCATCGGGCCATAGGTTTGAGTACTCAGCAGACCCACACCGAATGCGTACGCCGCTTTGTTTACCGGACCGCCCATGt                                                                                                                 <  2:1221701/140‑1 (MQ=255)                      cgcCACCATTGTTGCCAGACCAATTGCCAGCGGTGGCACCATACCTGCCGCCATAATCGCCGCCATCGGGCCATAGGTTTGAGTACTCAGCAGACCCACACCGAATGCGTACGCCGCTTTGTTTACCGGACCGCCCATGt                                                                                                                 <  2:729106/140‑1 (MQ=255)                      cgcCACCATTCTTGTCAGACCCATTGCCAGCGGTGGCACCATACCTGCCGCCATAATCGCCGCCATCGGGCCATAGGTTTGAGTACTCAGCAGACCCACACCGAATGCGTACGCCGCTTTGTTTACCGGACCGCCCATGt                                                                                                                 <  2:868721/140‑1 (MQ=255)                           ccaTTGTTGCCAGACCCATTGCCAGCGGTGGCACCATACCTGCCGCCATAATCGCCGCCATCGGGCCATAGGTTTGAGTACTCAGCAGACCCACACCGAATGCGTACGCCGCTTTGTTTACCGGACCGCCCATGTCAGTa                                                                                                            <  2:583171/140‑1 (MQ=255)                           ccaTTGTTGCCAGACCCATTGCCAGCGGTGGCACCATACCTGCCGCCATAATCGCCGCCATCGGGCCATAGGTTTGAGTACTCAGCAGACCCACACCGAATGCGTACGCCGCTTTGTTTACCGGACCGCCCATGTCAGTa                                                                                                            <  1:88397/140‑1 (MQ=255)                           ccaTTGTTGCCAGACCCATTGCCAGCGGTGGCACCATACCTGCCGCCATAATCGCCGCCATCGGGCCATAGGTTTGAGTACTCAGCAGACCCACACCGAATGCGTACGCCGCTTTGTTTACCGGACCGCCCATGTCAGTa                                                                                                            <  1:110294/140‑1 (MQ=255)                                gttgCCAGACCCATTGCCAGCGGTGGCACCATACCTGCCGCCATAATCGCCGCCATCGGGCCATAGGTTTGAGTACTCAGCAGACCCACACCGAATGCGTACGCCGCTTTGTTTACCGGACCGCCCATGTCAGTACAcat                                                                                                       <  1:1111923/140‑1 (MQ=255)                                 ttgCCAGACCCATTGCCAGCGGTGGCACCATACCTGCCGCCATAATCGCCGCCATCGGGCCATAGGTTTGAGTACTCAGCAGACCCACACCGAATGCGTACGCCGCTTTGTTTACCGGACCGCCCATGTCAGTACAcatc                                                                                                      >  1:485883/1‑140 (MQ=255)                                      aGACCCATTGCCAGCGGTGGCACCATACCTGCCGCCATAATCGCCGCCATCGGGCCATAGGTTTGAGTACTCAGCAGACCCACACCGAATGCGTACGCCGCTTTGTTTACCGGACCGCCCATGTCAGTACACATCATGcc                                                                                                 >  1:1240936/1‑140 (MQ=255)                                        aCCCATTGCCAGCGGTGGCACCATACCTGCCGCCATAATCGCCGCCATCGGGCCATAGGTTTGAGTACTCAGCAGACCCACACCGAATGCGTACGCCGCTTTGTTTACCGGACCGCCCATGTCAGTACACATCATGCCAc                                                                                               >  1:1007726/1‑140 (MQ=255)                                        aCCCATTGCCAGCGGTGGCACCATACCTGCCGCCATAATCGCCGCCATCGGGCCATAGGTTTGAGTACTCAGCAGACCCACACCGAATGCGTACGCCGCTTTGTTTACCGGACCGCCCATGTCAGTACACATCATGCCAc                                                                                               >  2:883698/1‑140 (MQ=255)                                        aCCCATTGCCAGCGGTGGCACCATACCTGCCGCCATAATCGCCGCCATCGGGCCATAGGTTTGAGTACTCAGCAGACCCACACCGAATGCGTACGCCGCTTTGTTTACCGGACCGCCCATGTCAGTACACATCATGCCAc                                                                                               >  2:518876/1‑140 (MQ=255)                                        aCCCATTGCCAGCGGTGGCACCATACCTGCCGCCATAATCGCCGCCATCGGGCCATAGGTTTGAGTACTCAGCAGACCCACACCGAATGCGTACGCCGCTTTGTTTACCGGACCGCCCATGTCAGTACACATCATGCCAc                                                                                               >  2:379472/1‑140 (MQ=255)                                                   gCGGTGGCACCATACCTGCCGCCATAATCGCCGCCATCGGGCCATAGGTTTGAGTACTCAGCAGACCCACACCGAATGCGTACGCCGCTTTGTTTACCGGACCGCCCATGTCAGTACACATCATGCCACCGAGGATCGcc                                                                                    <  1:311995/140‑1 (MQ=255)                                                    cGGTGGCACCATACCTGCCGCCATAATCGCCGCCATCGGGCCATAGGTTTGAGTACTCAGCAGACCCACACCGAATGCGTACGCCGCTTTGTTTACCGGACCGCCCATGTCAGTACACATCATGCCACCGAGGATCGccc                                                                                   >  1:1040493/1‑140 (MQ=255)                                                     ggTGGCACCATACCTGCCGCCATAATCGCCGCCATCGGGCCATAGGTTTGAGTACTCAGCAGACCCACACCGAATGCGTACGCCGCTTTGTTTACCGGACCGCCCATGTCAGTACACATCATGCCACCGAGGATCGcccc                                                                                  <  1:518876/140‑1 (MQ=255)                                                      gggcccccATCCCTCCCGCCATAATCGCCGCCATCGGGCCATAGGTTTGAGTACTCAGCAGACCCACACCGAATGCGTACGCCGCTTTGTTTACCGGACCGCCCATGTCAGTACACATCATGCCACCGAGGATCGccccc                                                                                 <  2:1084590/134‑1 (MQ=255)                                                      gTGGCACCATACCTGCCGCCATAATCGCCGCCATCGGGCCATAGGTTTGAGTACTCAGCAGACCCACACCGAATGCGTACGCCGCTTTGTTTACCGGACCGCCCATGTCAGTACACATCATGCCACCGAGGATCGccccc                                                                                 >  1:86345/1‑140 (MQ=255)                                                           aCCATACCTGCCGCCATAATCGCCGCCATCGGGCCATAGGTTTGAGTACTCAGCAGACCCACACCGAATGCGTACGCCGCTTTGTTTACCGGACCGCCCATGTCAGTACACATCATGCCACCGAGGATCGCCCCcagcag                                                                            <  1:394772/140‑1 (MQ=255)                                                            ccATACCTGCCGCCATAATCGCCGCCATCGGGCCATAGGTTTGAGTACTCAGCAGACCCACACCGAATGCGTACGCCGCTTTGTTTACCGGACCGCCCATGTCAGTACACATCATGCCACCGAGGATCGCCCCCAGCAGa                                                                           <  2:371174/140‑1 (MQ=255)                                                              aTACCTGCCGCCATAATCGCCGCCATCGGGCCATAGGTTTGAGTACTCAGCAGACCCACACCGAATGCGTACGCCGCTTTGTTTACCGGACCGCCCATGTCAGTACACATCATGCCACCGAGGATCGCCCCCAGCAGAAc                                                                         <  2:100320/140‑1 (MQ=255)                                                               tACCTGCCGCCATAATCGCCGCCATCGGGCCATAGGTTTGAGTACTCAGCAGACCCACACCGAATGCGTACGCCGCTTTGTTTACCGGACCGCCCATGTCAGTACACATCATGCCACCGAGGa                                                                                         <  1:602779/123‑1 (MQ=255)                                                               tACCTGCCGCCATAATCGCCGCCATCGGGCCATAGGTTTGAGTACTCAGCAGACCCACACCGAATGCGTACGCCGCTTTGTTTACCGGACCGCCCATGTCAGTACACATCATGCCACCGAGGa                                                                                         >  2:602779/1‑123 (MQ=255)                                                               tACCTGCCGCCATAATCGCCGCCATCGGGCCATAGGTTTGAGTACTCAGCAGACCCACACCGAATGCGTACGCCGCTTTGTTTACCGGACCGCCCATGTCAGTACACATCATGCCACCGAGGATCGCCCCCAGCAGAAcc                                                                        >  1:1177526/1‑140 (MQ=255)                                                                    gccgccATAATCGCCGCCATCGGGCCATAGGTTTGAGTACTCAGCAGACCCACACCGAATGCGTACGCCGCTTTGTTTACCGGACCGCCCATGTCAGTACACATCATGCCACCGAGGATCGCCCCCAGCAGAACCGCAtt                                                                   >  2:1348164/1‑140 (MQ=255)                                                                    gccgccATAATCGCCGCCATCGGGCCATAGGTTTGAGTACTCAGCAGACCCACACCGAATGCGTACGCCGCTTTGTTTACCGGACCGCCCATGTCAGTACACATCATGCCACCGAGGATCGCCCCCAGCAGAACCGCAtt                                                                   >  2:1315063/1‑140 (MQ=255)                                                                      cgccATAATCGCCGCCATCGGGCCATAGGTTTGAGTACTCAGCAGACCCACACCGAATGCGTACGCCGCTTTGTTTACCGGACCGCCCATGTCAGTACACATCATGCCACCGAGGATCGCCCCCAGCAGAACCGCATTCg                                                                 <  2:1186377/140‑1 (MQ=255)                                                                       gccATAATCGCCGCCATCGGGCCATAGGTTTGAGTACTCAGCAGACCCACACCGAATGCGTACGCCGCTTTGTTTACCGGACCGCCCATGTCAGTACACATCATGCCACCGAGGATCGCCCCCAGCAGAACCGCATTCGc                                                                >  1:486135/1‑140 (MQ=255)                                                                       gccATAATCGCCGCCATCGGGCCATAGGTTTGAGTACTCAGCAGACCCACACCGAATGCGTACGCCGCTTTGTTTACCGGACCGCCCATGTCAGTACACATCATGCCACCGAGGATCGCCCCCAGCAGAACCGCATTCGc                                                                <  1:715276/140‑1 (MQ=255)                                                                         cATAATCGCCGCCATCGGGCCATAGGTTTGAGTACTCAGCAGACCCACACCGAATGCGTACGCCGCTTTGTTTACCGGACCGCCCATGTCAGTACACATCATGCCACCGAGGATCGCCCCCAGCAGAACCGCATTCGCAg                                                              <  2:848976/140‑1 (MQ=255)                                                                           tAATCGCCGCCATCGGGCCATAGGTTTGAGTACTCAGCAGACCCACACCGAATGCGTACGCCGCTTTGTTTACCGGACCGCCCATGTCAGTACACATCATGCCACCGAGGATCGCCCCCAGCAGAACCGCATTCGCAGTc                                                            <  1:54691/140‑1 (MQ=255)                                                                                gccgccATCGGGCCATAGGTTTGAGTACTCAGCAGACCCACACCGAATGCGTACGCCGCTTTGTTTACCGGACCGCCCATGTCAGTACACATCATGCCACCGAGGATCGCCCCCAGCAGAACCGCATTCGCAGTCCCCAt                                                       >  1:538756/1‑140 (MQ=255)                                                                                gccgccATCGGGCCATAGGTTTGAGTACTCAGCAGACCCACACCGAATGCGTACGCCGCTTTGTTTACCGGACCGCCCATGTCAGTACACATCATGCCACCGAGGATCGCCCCCAGCAGAACCGCATTCGCAGTCCCCAt                                                       <  1:845062/140‑1 (MQ=255)                                                                                   gccATCGGGCCATAGGTTTGAGTACTCAGCAGACCCACACCGAATGCGTACGCCGCTTTGTTTACCGGACCGCCCATGTCAGTACACATCATGCCACCGAGGATCGCCCCCAGCAGAACCGCATTCGCAGTCCCCATGGt                                                    <  2:485883/140‑1 (MQ=255)                                                                                   gccATCGGGCCATAGGTTTGAGTACTCAGCAGACCCACACCGAATGCGTACGCCGCTTTGTTTACCGGACCGCCCATGTCAGTACACATCATGCCACCGAGGATCGCCCCCAGCAGAACCGCATTCGCAGTCCCCATGGt                                                    >  2:558668/1‑140 (MQ=255)                                                                                          ggCCATAGGTTTGAGTACTCAGCAGACCCACACCGAATGCGTACGCCGCTTTGTTTACCGGACCGCCCATGTCAGTACACATCATGCCACCGAGGATCGCCCCCAGCAGAACCGCATTCGCAGTCCCCATGGTCTGCAGc                                             <  1:883698/140‑1 (MQ=255)                                                                                            ccATAGGTTTGAGTACTCAGCAGACCCACACCGAATGCGTACGCCGCTTTGTTTACCGGACCGCCCATGTCAGTACACATCATGCCACCGAGGATCGCCCCCAGCAGAACCGCATTCGCAGTCCCCATGGTCTGCAGCCa                                           <  2:1177526/140‑1 (MQ=255)                                                                                              aTAGGTTTGAGTACTCAGCAGACCCACACCGAATGCGTACGCCGCTTTGTTTACCGGACCGCCCATGTCAGTACACATCATGCCACCGAGGATCGCCCCCAGCAGAACCGCATTCGCAGTCCCCATGGTCTGCAGCCAGt                                         <  2:176318/140‑1 (MQ=255)                                                                                                   tttGAGTACTCAGCAGACCCACACCGACTGCGTACGCCGCTTTGTTTACCGGACCGCCCATGTCAGTACACATCATGCCACCGAGGATCGCCCCCAGCAGAACCGCATTCGCAGCCCCCATGGTCTGCAGCCAGTGAGTc                                    <  2:1362765/140‑1 (MQ=255)                                                                                                   tttGAGTACTCAGCAGACCCACACCGAATGCGTACGCCGCTTTGTTTACCGGACCGCCCATGTCAGTACACATCATGCCACCGAGGATCGCCCCCAGCAGAACCGCATTCGCAGTCCCCATGGTCTGCAGCCAGTGAGTc                                    <  2:1007726/140‑1 (MQ=255)                                                                                                   tttGAGTACTCAGCAGACCCACACCGAATGCGTACGCCGCTTTGTTTACCGGACCGCCCATGTCAGTACACATCATGCCACCGAGGATCGCCCCCAGCAGAACCGCATTCGCAGTCCCCATGGTCTGCAGCCAGTGAGTc                                    <  1:599644/140‑1 (MQ=255)                                                                                                    ttGAGTACTCAGCAGACCCACACCGAATGCGTACGCCGCTTTGTTTACCGGACCGCCCATGTCAGTACACATCATGCCACCGAGGATCGCCCCTAGCAGAACCGCATTCGCAGTCCCCATGGTGTGCAGCCAGTGAGTCa                                   >  2:1264740/1‑140 (MQ=255)                                                                                                     tGAGTACTCAGCAGACCCACACCGAATGCGTACGCCGCTTTGTTTACCGGACCGCCCATGTCAGTACACATCATGCCACCGAGGATCGCCCCCAGCAGAACCGCATTCGCAGTCCCCATGGTCTGCAGCCAGTGAGTCAg                                  >  2:622268/1‑140 (MQ=255)                                                                                                     tGAGTACTCAGCAGACCCACACCGAATGCGTACGCCGCTTTGTTTACCGGACCGCCCATGTCAGTACACATCATGCCACCGAGGATCGCCCCCAGCAGAACCGCATTCGCAGTCCCCATGGTCTGCAGCCAGTGAGTCAg                                  >  1:213293/1‑140 (MQ=255)                                                                                                     tGAGTACTCAGCAGACCCACACCGAATGCGTACGCCGCTTTGTTTACCGGACCGCCCATGTCAGTACACATCATGCCACCGAGGATCGCCCCCAGCAGAACCGCATTCGCAGTCCCCATGGTCTGCAGCCAGTGAGTCAg                                  >  1:699571/1‑140 (MQ=255)                                                                                                      gAGTACTCAGCAGACCCACACCGAATGCGTACGCCGCTTTGTTTACCGGACCGCCCATGTCAGTACACATCATGCCACCGAGGATCGCCCCCAGCAGAACCGCATTCGCAGTCCCCATGGTCTGCAGCCAGTGAGTCAGc                                 >  1:22734/1‑140 (MQ=255)                                                                                                       aGTACTCAGCAGACCCACACCGAATGCGTACGCCGCTTTGTTTACCGGACCGCCCATGTCAGTACACATCATGCCACCGAGGATCGCCCCCAGCAGAACCGCATTCGCAGTCCCCATGGTCTGCAGCCAGTGAGTc                                    <  2:530785/136‑1 (MQ=255)                                                                                                       aGTACTCAGCAGACCCACACCGAATGCGTACGCCGCTTTGTTTACCGGACCGCCCATGTCAGTACACATCATGCCACCGAGGATCGCCCCCAGCAGAACCGCATTCGCAGTCCCCATGGTCTGCAGCCAGTGAGTc                                    >  1:530785/1‑136 (MQ=255)                                                                                                              agcagACCCACACCGAATGCGTACGCCGCTTTGTTTACCGGACCGCCCATGTCAGTACACATCATGCCACCGAGGATCGCCCCCAGCAGAACCGCATTCGCAGTCCCCATGGTCTGCAGCCAGTGAGTCAGCCCTTCgag                         >  1:1200494/1‑140 (MQ=255)                                                                                                                cagACCCACACCGAATGCGTACGCCGCTTTGTTTACCGGACCGCCCATGTCAGTACACATCATGCCACCGAGGATCGCCCCCAGCAGAACCGCATTCGCAGTCCCCATGGTCTGCAGCCAGTGAGTCAGCCCTTCGAGaa                       <  1:981407/140‑1 (MQ=255)                                                                                                                 agACCCACACCGAATGCGTACGCCGCTTTGTTTACCGGACCGCCCATGTCAGTACACATCATGCCACCGAGGATCGCCCCCAGCAGAACCGCATTCGCAGTCCCCATGGTCTGCAGCCAGTGAGTCAGCCCTTCGAGAAt                      <  1:1185091/140‑1 (MQ=255)                                                                                                                   aCCCACACCGAATGCGTACGCCGCTTTGTTTACCGGACCGCCCATGTCAGTACACATCATGCCACCGAGGATCGCCCCCAGCAGAACCGCATTCGCAGTCCCCATGGTCTGCAGCCAGTGAGTCAGCCCTTCGAGAATGc                    >  1:345604/1‑140 (MQ=255)                                                                                                                       acacCGAATGCGTACGCCGCTTTGTTTACCGGACCGCCCATGTCAGTACACATCATGCCACCGAGGATCGCCCCCAGCAGAACCGCATTCGCAGTCCCCATGGTCTGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGc                <  2:486135/140‑1 (MQ=255)                                                                                                                        cacCGAATGCGTACGCCGCTTTGTTTACCGGACCGCCCATGTCAGTACACATCATGCCACCGAGGATCGCCCCCAGCAGAACCGCATTCGCAGTCCCCATGGTCTGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGc                <  2:357635/139‑1 (MQ=255)                                                                                                                        cacCGAATGCGTACGCCGCTTTGTTTACCGGACCGCCCATGTCAGTACACATCATGCCACCGAGGATCGCCCCCAGCAGAACCGCATTCGCAGTCCCCATGGTCTGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGc                >  1:357635/1‑139 (MQ=255)                                                                                                                         acCGAATGCGTACGCCGCTTTGTTTACCGGACCGCCCATGTCAGTACACATCATGCCACCGAGGATCGCCCCCAGCAGAACCGCATTCGCAGTCCCCATGGTCTGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCaa              <  2:699571/140‑1 (MQ=255)                                                                                                                             aaTGCGTACGCCGCTTTGTTTACCGGACCGCCCATGTCAGTACACATCATGCCACCGAGGATCGCCCCCAGCAGAACCGCATTCGCAGTCCCCATGGTCTGCAGCCAGTGAGTCAGCCCTTCGAGAATg                     <  2:660413/129‑1 (MQ=255)                                                                                                                             aaTGCGTACGCCGCTTTGTTTACCGGACCGCCCATGTCAGTACACATCATGCCACCGAGGATCGCCCCCAGCAGAACCGCATTCGCAGTCCCCATGGTCTGCAGCCAGTGAGTCAGCCCTTCGAGAATg                     >  1:660413/1‑129 (MQ=255)                                                                                                                             aaTGCGTACGCCGCTTTGTTTACCGGACCGCCCATGTCAGTACACATCATGCCACCGAGGATCGCCCCCAGCAGAACCGCATTCGCAGTCCCCATGGTCTGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTgg          >  1:1013362/1‑140 (MQ=255)                                                                                                                             aaTGCGTACGCCGCTTTGTTTACCGGACCGCCCATGTCAGTACACATCATGCCACCGAGGATCGCCCCCAGCAGAACCGCATTCGCAGTCCCCATGGTCTGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTgg          >  1:1222734/1‑140 (MQ=255)                                                                                                                             aaTGCGTACGCCGCTTTGTTTACCGGACCGCCCATGTCAGTACACATCATGCCACCGAGGATCGCCCCCAGCAGAACCGCATTCGCAGTCCCCATGGTCTGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTgg          >  2:1186722/1‑140 (MQ=255)                                                                                                                              aTGCGTACGCCGCTTTGTTTACCGGACCGCCCATGTCAGTACACATCATGCCACCGAGGATCGCCCCCAGCAGAACCGCATTCGCAGTCCCCATGGTCTGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGt         >  1:75753/1‑140 (MQ=255)                                                                                                                               tGCGTACGCCGCTTTGTTTACCGGACCGCCCATGTCAGTACACATCATGCCACCGAGGATCGCCCCCAGCAGAACCGCATTCGCAGTCCCCATGGTCTGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGtt        >  1:965102/1‑140 (MQ=255)                                                                                                                               tGCGTACGCCGCTTTGTTTACCGGACCGCCCATGTCAGTACACATCATGCCACCGAGGATCGCCCCCAGCAGAACCGCATTCGCAGTCCCCATGGTCTGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGtt        <  1:1348515/140‑1 (MQ=255)                                                                                                                                gCGTACGCCGCTTTGTTTACCGGACCGCCCATGTCAGTACACATCATGCCACCGAGGATCGCCCCCAGCAGAACCGCATTCGCAGTCCCCATGGTCTGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGttt       <  1:1315063/140‑1 (MQ=255)                                                                                                                                 cGTACGCCGCTTTGTTTACCGGACCGCCCATGTCAGTACACATCATGCCACCGAGGATCGCCCCCAGCAGAACCGCATTCGCAGTCCCCATGGTCTGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTa      <  2:860625/140‑1 (MQ=255)                                                                                                                                    aCGCCGCTTTGTTTACCGGACCGCCCATGTCAGTACACATCATGCCACCGAGGATCGCCCCCAGCAGAACCGCATTCGCAGTCCCCATGGTCTGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCg   <  1:1186722/140‑1 (MQ=255)                                                                                                                                     cgccgcTTTGTTTACCGGACCGCCCATGTCAGTACACATCATGCCACCGAGGATCGCCCCCAGCAGAACCGCATTCGCAGTCCCCATGGTCTGCAGCCAGTGAGTCAGCCCTTCGGGAATGCCAGCAACTGGTTTACCGa  <  2:465233/140‑1 (MQ=255)                                                                                                                                     cgccgcTTTGTTTACCGGACCGCCCATGTCAGTACACATCATGCCACCGAGGATCGCCCCCAGCAGAACCGCATTCGCAGTCCCCATGGTCTGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGa  <  2:1200494/140‑1 (MQ=255)                                                                                                                                     cgccgcTTTGTTTACCGGACCGCCCATGTCAGTACACATCATGCCACCGAGGATCGCCCCCAGCAGAACCGCATCCGCAGTCCCCATGGTCTGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGa  <  2:1240936/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                         CGCTTTGTCGAATTTGCGACGCGCCACCATTGTTGCCAGACCCATTGCCAGCGGTGGCACCATACCTGCCGCCATAATCGCCGCCATCGGGCCATAGGTTTGAGTACTCAGCAGACCCACACCGAATGCGTACGCTGCTTTGTTTACCGGACCGCCCATGTCAGTACACATCATGCCACCGAGGATCGCCCCCAGCAGAACCGCATTCGCAGTCCCCATGGTCTGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGA  >  NC_000913/2260037‑2260308

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑