Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,262,028	A→G	V113A (GTC→GCC)	fruK ←	1‑phosphofructokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,262,028	0	A	G	100.0%	245.6 / NA	72	V113A (GTC→GCC)	fruK	1‑phosphofructokinase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (40/32);  total (40/32)

CGCAGGCGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCGAGCCAGCTCAGAGAATCAGTCACAAAGCGTTCCCAGTCGGCGGGGGTGACTTCAAAACCCGAGAAGTTGAAGTCGGTCACTTCGCCGTCTTTTTCCGTCAGCTTAACGTTAATTCGGGTGCGCCCCTGTACAACCTGGAAACGGTTGGCAATGCCCAGCTCGCTGAACAGTTGCTGAAAA  >  NC_000913/2261893‑2262159                                                                                                                                        |                                                                                                                                    cGCAGGCGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCGAGCCAGCTCGGAGAATCAGTCACAAAGCGTTCCCAGTCGGCGGGGGTGGCTTc                                                                                                                                 >  1:1041261/1‑140 (MQ=255) cGCAGGCGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCGAGCCAGCTCAGAGAATCAGTCACAAAGCGTTCCCAGTCGGCGGGGGTGGCTTc                                                                                                                                 >  1:1020028/1‑140 (MQ=255) cGCAGGCGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCGAGCCAGCTCAGAGAATCAGTCACAAAGCGTTCCCAGTCGGCGGGGGTGGCTTc                                                                                                                                 >  1:646775/1‑140 (MQ=255)  gCAGGCGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCGAGCCAGCTCAGAGAATCAGTCACAAAGCGTTCCCAGTCGGCGGGGGTGGCTTCa                                                                                                                                <  1:1248862/140‑1 (MQ=255)     ggCGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCGAGCCAGCTCAGAGAATCAGTCACAAAGCGTTCCCAGTCGGCGGGGGTGGTTTCaaaa                                                                                                                             >  2:354536/1‑140 (MQ=255)     ggCGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCGAGCCAGCTCAGAGAATCAGTCACAAAGCGTTCCCAGTCGGCGGGGGTGGCTTCaaaa                                                                                                                             >  2:1331687/1‑140 (MQ=255)      gCGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCGAGCCAGCTCAGAGAATCAGTCATAAAGCGTTCCCAGTCGGCGGGGGTGGCTTCAAAAc                                                                                                                            <  1:1290703/140‑1 (MQ=255)      gCGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCGAGCCAGCTCAGAGAATCAGTCACAAAGCGTTCCCAGTCGGCGGGGGTGGCTTCAAAAc                                                                                                                            >  2:101347/1‑140 (MQ=255)      gCGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCGAGCCAGCTCAGAGAATCAGTCACAAAGCGTTCCCAGTCGGCGGGGGTGGCTTCAAAAc                                                                                                                            >  2:342236/1‑140 (MQ=255)       cGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCGAGCCAGCTCAGAGAATCAGTCACAAAGCGTTCCCAGTCGGCGGGGGTGGCTTCAAAAcc                                                                                                                           >  2:1125091/1‑140 (MQ=255)       cGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCGAGCCAGCTCAGAGAATCAGTCACAAAGCGTTCCCAGTCGGCGGGGGTGGCTTCAAAAcc                                                                                                                           >  1:70269/1‑140 (MQ=255)                    cGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCGAGCCAGCTCAGAGAATCAGTCACAAAGCGTTCCCAGTCGGCGGGGGTGGCTTCAAAACCCGAGAAGTTGAAg                                                                                                              <  1:362558/140‑1 (MQ=255)                        gAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCGAGCCAGCTCAGAGAATCAGTCACAAAGCGTTCCCAGTCGGCGGGGGTGGCTTCAAAACCCGAGAAGTTGAAGTCgg                                                                                                          >  2:722267/1‑140 (MQ=255)                         aaCGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCGAGCCAGCTCAGAGAATCAGTCACAAAGCGTTCCCAGTCGGCGGGGGTGGCTTCAAAACCCGAGAAGTTGAAGTCGGt                                                                                                         >  1:486556/1‑140 (MQ=255)                            gCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCGAGCCAGCTCAGAGAATCAGTCACAAAGCGTTCCCAGTCGGCGGGGGTGGCTTCAAAACCCGAGAAGTTGAAGTCGGTCAc                                                                                                      >  1:733649/1‑140 (MQ=255)                                ccGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCGAGCCAGCTCAGAGAATCAGTCACAAAGCGTTCCCAGTCGGCGGGGGTGGCTTCAAAACCCGAGAAGTTGAAGTCGGTCACTTCg                                                                                                  <  1:61173/140‑1 (MQ=255)                                ccGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCGAGCCAGCTCAGAGAATCAGTCACAAAGCGTTCCCAGTCGGCGGGGGTGGCTTCAAAACCCGAGAAGTTGAAGTCGGTCACTTCg                                                                                                  <  1:580025/140‑1 (MQ=255)                                 cGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCGAGCCAGCTCAGAGAATCAGTCACAAAGCGTTCCCAGTCGGCGGGGGTGGCTTCAAAACCCGAGAAGTTGAAGTCGGTCACTTCGc                                                                                                 <  1:1125091/140‑1 (MQ=255)                                     cTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCGAGCCAGCTCAGAGAATCAGTCACAAAGCGTTCCCAGTCGGCGGGGGTGGCTTCAAAACCCGAGAAGTTGAAGTCGGTCACTTCGCCGTc                                                                                             >  2:690662/1‑140 (MQ=255)                                        aCGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCGAGCCAGCTCAGAGAATCAGTCACAAAGCGTTCCCAGTCGGCGGGGGTGGCTTCAAAACCCGAGAAGTTGAAGTCGGTCACTTCGCCGTCttt                                                                                          >  2:1406713/1‑140 (MQ=255)                                        aCGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCGAGCCAGCTCAGAGAATCAGTCACAAAGCGTTCCCAGTCGGCGGGGGTGGCTTCAAAACCCGAGAAGTTGAAGTCGGTCACTTCGCCGTCttt                                                                                          >  1:566977/1‑140 (MQ=255)                                             tGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCGAGCCAGCTCAGAGAATCAGTCACAAAGCGTTCCCAGTCGGCGGGGGTGGCTTCAAAACCCGAGAAGTTGAAGTCGGTCACTTCGCCGTCTTTTTCCg                                                                                     <  2:1005701/140‑1 (MQ=255)                                              gACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCGAGCCAGCTCAGAGAATCAGTCACAAAGCGTTCCCAGTCGGCGGGGGTGGTTTCAAAACCCGAGAAGTTGAAGTCGGTCACTTCGCCGTCTTTTTCCGt                                                                                    <  1:354536/140‑1 (MQ=255)                                              gACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCGAGCCAGCTCAGAGAATCAGTCACAAAGCGTTCCCAGTCGGCGGGGGTGGCTTCAAAACCCGAGAAGTTGAAGTCGGTCACTTCGCCGTCTTTTTCCGt                                                                                    <  1:342236/140‑1 (MQ=255)                                                         tCCGCTGACACAGACCATATCGAACTGACCGAGCCAGCTCAGAGAATCAGTCACAAAGCGTTCCCAGTCGGCGGGGGTGGCTTCAAAACCCGAGAAGTTGAAGTCGGTCACTTCGCCGTCTTTTTCCGTCAGCTTAACGt                                                                         >  2:370405/1‑140 (MQ=255)                                                                acacAGACCATATCGAACTGACCGAGCCAGCTCAGAGAATCAGTCACAAAGCGTTCCCAGTCGGCGGGGGTGGCTTCAAAACCCGGGAAGTTGAAGGCGGTCACTTCGCCGTCCTTTTCCGTCAGCTTAACGTTAATTCg                                                                  >  1:688949/1‑140 (MQ=255)                                                                 cacacaCCATATCGAACTGACCGAGCCAGCTCAGAGAATCAGTCACAAAGCGTTCCCAGTCGGCGGGGGCGGCTTCAAAACCCGCGCAGTTGAAATCGGTCACTTCGCCGTCTTTTTCCGTTAGCTTTACGTTAATTCgg                                                                 >  2:1016530/1‑140 (MQ=255)                                                                    aGACCATATCGAACTGACCGAGCCAGCTCAGAGAATCAGTCACAAAGCGTTCCCAGTCGGCGGGGGTGGCTTCAAAACCCGAGAAGTTGAAGTCGGTCACTTCGCCGTCTTTTTCCGTCAGCTTAACGTTAATTCGGGTg                                                              <  2:379619/140‑1 (MQ=255)                                                                    aGACCATATCGAACTGACCGAGCCAGCTCAGAGAATCAGTCACAAAGCGTTCCCAGTCGGCGGGGGTGGCTTCAAAACCCGAGAAGTTGAAGTCGGTCACTTCGCCGTCTTTTTCCGTCAGCTTAACGTTAATTCGGGTg                                                              <  2:1187706/140‑1 (MQ=255)                                                                    aGACCATATCGAACTGACCGAGCCAGCTCAGAGAATCAGTCACAAAGCGTTCCCAGTCGGCGGGGGTGGCTTCAAAACCCGAGAAGTTGAAGTCGGTCACTTCGCCGTCTTTTTCCGTCAGCTTAACGTTAATTCGGGTg                                                              <  2:733649/140‑1 (MQ=255)                                                                    aGACCATATCGAACTGACCGAGCCAGCTCAGAGAATCAGTCACAAAGCGTTCCCAGTCGGCGGGGGTGGCTTCAAAACCCGAGAAGTTGAAGTCGGTCACTTCGCCGTCTTTTTCCGTCAGCTTAACGTTAATTCGGGTg                                                              <  2:1396394/140‑1 (MQ=255)                                                                      aCCATATCGAACTGACCGAGCCAGCTCAGAGAATCAGTCACAAAGCGTTCCCAGTCGGCGGGGGTGGCTTCAAAACCCGAGAAGTTGAAGTCGGTCACTTCGCCGTCTTTTTCCGTCAGCTTAACGTTAATTCGGGTgcg                                                            <  1:1088457/140‑1 (MQ=255)                                                                      aCCATATCGAACTGACCGAGCCAGCTCAGAGAATCAGTCACAAAGCGTTCCCAGTCGGCGGGGGTGGCTTCAAAACCCGAGAAGTTGAAGTCGGTCACTTCGCCGTCTTTTTCCGTCAGCTTAACGTTAATTCGGGTgcg                                                            >  1:1258774/1‑140 (MQ=255)                                                                      aCCATATCGAACTGACCGAGCCAGCTCAGAGAATCAGTCACAAAGCGTTCCCAGTCGGCGGGGGTGGCTTCAAAACCCGAGAAGTTGAAGTCGGTCACTTCGCCGTCTTTTTCCGTCAGCTTAACGTTAATTCGGGTg                                                              >  2:316331/1‑138 (MQ=255)                                                                      aCCATATCGAACTGACCGAGCCAGCTCAGAGAATCAGTCACAAAGCGTTCCCAGTCGGCGGGGGTGGCTTCAAAACCCGAGAAGTTGAAGTCGGTCACTTCGCCGTCTTTTTCCGTCAGCTTAACGTTAATTCGGGTg                                                              <  1:434661/138‑1 (MQ=255)                                                                      aCCATATCGAACTGACCGAGCCAGCTCAGAGAATCAGTCACAAAGCGTTCCCAGTCGGCGGGGGTGGCTTCAAAACCCGAGAAGTTGAAGTCGGTCACTTCGCCGTCTTTTTCCGTCAGCTTAACGTTAATTCGGGTg                                                              <  1:316331/138‑1 (MQ=255)                                                                      aCCATATCGAACTGACCGAGCCAGCTCAGAGAATCAGTCACAAAGCGTTCCCAGTCGGCGGGGGTGGCTTCAAAACCCGAGAAGTTGAAGTCGGTCACTTCGCCGTCTTTTTCCGTCAGCTTAACGTTAATTCGGGTg                                                              >  2:434661/1‑138 (MQ=255)                                                                       ccATATCGAACTGACCGAGCCAGCTCAGAGAATCAGTCACAAAGCGTTCCCAGTCGGCGGGGGTGGCTTCAAAACCCGAGAAGTTGAAGTCGGTCACTTCGCCGTCTTTTTCCGTCAGCTTAACGTTAATTCGGGTgcgc                                                           >  2:1168074/1‑140 (MQ=255)                                                                        cATATCGAACTGACCGAGCCAGCTCAGAGAATCAGTCACAAAGCGTTCCCAGTCGGCGGGGGTGGCTTCAAAACCCGAGAAGTTGAAGTCGGTCACTTCGCCGTCTTTTTCCGTCAGCTTAACGTTAATTCGGGTGCGcc                                                          <  2:407645/140‑1 (MQ=255)                                                                        cATATCGAACTGACCGAGCCAGCTCAGAGAATCAGTCACAAAGCGTTCCCAGTCGGCGGGGGTGGCTTCAAAACCCGAGAAGTTGAAGTCGGTCACTTCGCCGTCTTTTTCCGTCAGCTTAACGTTAATTCGGGTGCGcc                                                          <  2:76845/140‑1 (MQ=255)                                                                        cATATCGAACTGACCGAGCCAGCTCAGAGAATCAGTCACAAAGCGTTCCCAGTCGGCGGGGGTGGCTTCAAAACCCGAGAAGTTGAAGTCGGTCACTTCGCCGTCTTTTTCCGTCAGCTTAACGTTAATTCGGGTGCGcc                                                          <  1:11462/140‑1 (MQ=255)                                                                        cATATCGAACTGACCGAGCCAGCTCAGAGAATCAGTCACAAAGCGTTCCCAGTCGGCGGGGGTGGCTTCAAAACCCGAGAAGTTGAAGTCGGTCACTTCGCCGTCTTTTTCCGTCAGCTTAACGTTAATTCGGGTGCGcc                                                          <  1:1166640/140‑1 (MQ=255)                                                                              gAACTGACCGAGCCAGCTCAGAGAATCAGTCACAAAGCGTTCCCAGTCGGCGGGGGTGGCTTCAAAACCCGAGAAGTTGAAGTCGGTCACTTCGCCGTCTTTTTCCGTCAGCTTAACGTTAATTCGGGTGCGCCCCTGTa                                                    <  2:646775/140‑1 (MQ=255)                                                                              gAACTGACCGAGCCAGCTCAGAGAATCAGTCACAAAGCGTTCCCAGTCGGCGGGGGTGGCTTCAAAACCCGAGAAGTTGAAGTCGGTCACTTCGCCGTCTTTTTCCGTCAGCTTAACGTTAATTCGGGTGCGCCCCTGTa                                                    <  2:1258774/140‑1 (MQ=255)                                                                              gAACTGACCGAGCCAGCTCAGAGAATCAGTCACAAAGCGTTCCCAGTCGGCGGGGGTGGCTTCAAAACCCGAGAAGTTGAAGTCGGTCACTTCGCCGTCCTTTTCCGTCAGCTTAACGTTAATTCGGGTGCGCCCCTGTa                                                    <  2:688949/140‑1 (MQ=255)                                                                                aCTGACCGAGCCAGCTCAGAGAATCAGTCACAAAGCGTTCCCAGTCGGCGGGGGTGGCTTCAAAACCCGAGAAGTTGAAGTCGGTCACTTCGCCGTCTTTTTCCGTCAGCTTAACGTTAATTCGGGTGCGCCCCTGTACa                                                  >  2:1115166/1‑140 (MQ=255)                                                                                aCTGACCGAGCCAGCTCAGAGAATCAGTCACAAAGCGTTCCCAGTCGGCGGGGGTGGCTTCAAAACCCGAGAAGTTGAAGTCGGTCACTTCGCCGTCTTTTTCCGTCAGCTTAACGTTAATTCGGGTGCGCCCCTGTACa                                                  >  1:1143149/1‑140 (MQ=255)                                                                                  tGACCGAGCCAGCTCAGAGAATCAGTCACAAAGCGTTCCCAGTCGGCGGGGGTGGCTTCAAAACCCGAGAAGTTGAAGTCGGTCACTTCGCCGTCTTTTTCCGTCAGCTTAACGTTAATTCGGGTGCGCCCCTGTACAAc                                                <  1:724772/140‑1 (MQ=255)                                                                                    aCCGAGCCAGCTCAGAGAATCAGTCACAAAGCGTTCCCAGTCGGCGGGGGTGGCTTCAAAACCCGAGAAGTTGAAGTCGGTCACTTCGCCGTCTTTTTCCGTCAGCTTAACGTTAATTCGGGTGCGCCCCTGTACAACCt                                              >  2:526505/1‑140 (MQ=255)                                                                                    aCCGAGCCAGCTCAGAGAATCAGTCACAAAGCGTTCCCAGTCGGCGGGGGTGGCTTCAAAACCCGAGAAGTTGAAGTCGGTCACTTCGCCGTCTTTTTCCGTCAGCTTAACGTTAATTCGGGTGCGCCCCTGTACAACCt                                              >  2:586056/1‑140 (MQ=255)                                                                                      cGAGCCAGCTCAGAGAATCAGTCACAAAGCGTTCCCAGTCGGCGGGGGTGGCTTCAAAACCCGAGAAGTTGAAGTCGGTCACTTCGCCGTCTTTTTCCGTCAGCTTAACGTTAATTCGGGTGCGCCCCTGTACAACCTgg                                            <  2:70269/140‑1 (MQ=255)                                                                                             gCTCAGAGAATCAGTCACAAAGCGTTCCCAGTCGGCGGGGGTGGCTTCAAAACCCGAGAAGTTGAAGTCGGTCACTTCGCCGTCTTTTTCCGTCAGCTTAACGTTAATTCGGGTGCGCCCCTGTACAACCTGGAAACGGt                                     <  2:262796/140‑1 (MQ=255)                                                                                             gCTCAGAGAATCAGTCACAAAGCGTTCCCAGTCGGCGGGGGTGGCTTCAAAACCCGAGAAGTTGAAGTCGGTCACTTCGCCGTCTTTTTCCGTCAGCTTAACGTTAATTCGGGTGCGCCCCTGTACAACCTGGAAACGGt                                     <  2:566977/140‑1 (MQ=255)                                                                                              cTCAGAGAATCAGTCACAGAGCGTTCCCAGTCGGCGGGGGTGGCTTCAAAACCCGAGAAGTTGAAGTCGGTCACTTCGCCGTCTTTTTCCGTCAGCTTAACGTTAATTCGGGTGCGCCCCTGTACAACCTGGAAACGGtt                                    >  1:1113839/1‑140 (MQ=255)                                                                                              cTCAGAGAATCAGTCACAAAGCGTTCCCAGTCGGCGGGGGTGGCTTCAAAACCCGAGAAGTTGAAGTCGGTCACTTCGCCGTCTTTTTCCGTCAGCTTAACGTTAATTCGGGTGCGCCCCTGTACAACCTGGAAACGGtt                                    >  2:513736/1‑140 (MQ=255)                                                                                                   agaATCAGTCACAAAGCGTTCCCAGTCGGCGGGGGTGGCTTCAAAACCCGAGAAGTTGAAGTCGGTCACTTCGCCGTCTTTTTCCGTCAGCTTAACGTTAATTCGGGTGCGCCCCTGTACAACCTGGAAACGGTTGGCaa                               >  1:954150/1‑140 (MQ=255)                                                                                                   agaATCAGTCACAAAGCGTTCCCAGTCGGCGGGGGTGGCTTCAAAACCCGAGAAGTTGAAGTCGGTCACTTCGCCGTCTTTTTCCGTCAGCTTAACGTTAATTCGGGTGCGCCCCTGTACAACCTGGAAACGGTTGGCaa                               >  1:680717/1‑140 (MQ=255)                                                                                                       tCAGTCACAAAGCGTTCCCAGTCGGCGGGGGTGGCTTCAAAACCCGAGAAGTTGAAGTCGGTCACTTCGCCGTCTTTTTCCGTCAGCTTAACGTTAATTCGGGTGCGCCCCTGTACAACCTGGAAACGGTTGGCAATGcc                           >  1:439099/1‑140 (MQ=255)                                                                                                        cAGTCACAAAGCGTTCCCAGTCGGCGGGGGTGGCTTCAAAACCCGAGAAGTTGAAGTCGGTCACTTCGCCGTCTTTTTCCGTCAGCTTAACGTTAATTCGGGTGCGCCCCTGTACAACCTGGAAACGGTTGGCAATGccc                          >  2:603457/1‑140 (MQ=255)                                                                                                         aGTCACAAAGCGTTCCCAGTCGGCGGGGGTGGCTTCAAAACCCGAGAAGTTGAAGTCGGTCACTTCGCCGTCTTTTTCCGTCAGCTTAACGTTAATTCGGGTGCGCCCCTGTACAACCTGGAAACGGTTGGCAATGCCCa                         <  1:526505/140‑1 (MQ=255)                                                                                                         aGTCACAAAGCGTTCCCAGTCGGCGGGGGTGGCTTCAAAACCCGAGAAGTTGAAGTCGGTCACTTCGCCGTCTTTTTCCGTCAGCTTAACGTTAATTCGGGTGCGCCCCTGTACAACCTGGAAACGGTTGGCAATGCCCa                         >  2:1275156/1‑140 (MQ=255)                                                                                                                 aGCGTTCCCAGTCGGCGGGGGTGGCTTCAAAACCCGAGAAGTTGAAGTCGGTCACTTCGCCGTCTTTTTCCGTCAGCTTAACGTTAATTCGGGTGCGCCCCTGTACAACCTGGAAACGGTTGGCAATGCCCAGCTCGCTg                 >  1:669375/1‑140 (MQ=255)                                                                                                                   cGTTCCCAGTCGGCGGGGGTGGCTTCAAAACCCGAGAAGTTGAAGTCGGTCACTTCGCCGTCTTTTTCCGTCAGCTTAACGTTAATTCGGGTGCGCCCCTGTACAACCTGGAAACGGTTGGCAATGCCCAGCTCGCTGaa               <  1:603457/140‑1 (MQ=255)                                                                                                                     ttCCCAGTCGGCGGGGGTGGCTTCAAAACCCGAGAAGTTGAAGTCGGTCACTTCGCCGTCTTTTTCCGTCAGCTTAACGTTAATTCGGGTGCGCCCCTGTACAACCTGGAAACGGTTGGCAATGCCCAGCTCGCTGaa               <  1:1047152/138‑1 (MQ=255)                                                                                                                     ttCCCAGTCGGCGGGGGTGGCTTCAAAACCCGAGAAGTTGAAGTCGGTCACTTCGCCGTCTTTTTCCGTCAGCTTAACGTTAATTCGGGTGCGCCCCTGTACAACCTGGAAACGGTTGGCAATGCCCAGCTCGCTGaa               >  2:1047152/1‑138 (MQ=255)                                                                                                                     ttCCCAGTCGGCGGGGGTGGCTTCAAAACCCGAGAAGTTGAAGTCGGTCACTTCGCCGTCTTTTTCCGTCAGCTTAACGTTAATTCGGGTGCGCCCCTGTACAACCTGGAAACGGTTGGCAATGCCCAGCTCGCTGAACa             >  2:371262/1‑140 (MQ=255)                                                                                                                     ttCCCAGTCGGCGGGGGTGGCTTCAAAACCCGAGAAGTTGAAGTCGGTCACTTCGCCGTCTTTTTCCGTCAGCTTAACGTTAATTCGGGTGCGCCCCTGTACAACCTGGAAACGGTTGGCAATGCCCAGCTCGCTGAACa             >  1:377726/1‑140 (MQ=255)                                                                                                                     ttCCCAGTCGGCGGGGGTGGCTTCAAAACCCGAGAAGTTGAAGTCGGTCACTTCGCCGTCTTTTTCCGTCAGCTTAACGTTAATTCGGGTGCGCCCCTGTACAACCTGGAAACGGTTGGCAATGCCCAGCTCGCTGAACa             >  1:790495/1‑140 (MQ=255)                                                                                                                      tCCCAGTCGGCGGGGGTGGCTTCAAAACCCGAGAAGTTGAAGTCGGTCACTTCGCCGTCTTTTTCCGTCAGCTTAACGTTAATTCGGGTGCGCCCCTGTACAACCTGGAAACGGTTGGCAATGCCCAGCTCGCTGAACAg            <  1:371262/140‑1 (MQ=255)                                                                                                                       cccAGTCGGCGGGGGTGGCTTCAAAACCCGAGAAGTTGAAGTCGGTCACTTCGCCGTCTTTTTCCGTCAGCTTAACGTTAATTCGGGTGCGCCCCTGTACAACCTGGAAACGGTTGGCAATGCCCAGCTCGCTGAACAGt           <  2:1020028/140‑1 (MQ=255)                                                                                                                         cAGTCGGCGGGGGTGGCTTCAAAACCCGAGAAGTTGAAGTCGGTCACTTCGCCGTCTTTTTCCGTCAGCTTAACGTTAATTCGGGTGCGCCCCTGTACAACCTGGAAACGGTTGGCAATGCCCAGCTCGCTGAACAGTTg         >  1:1019675/1‑140 (MQ=255)                                                                                                                                cggGGGTGGCTTCAAAACCCGAGAAGTTGAAGTCGGTCACTTCGCCGTCTTTTTCCGTCAGCTTAACGTTAATTCGGGTGCGCCCCTGTACAACCTGGAAACGGTTGGCAATGCCCAGCTCGCTGAACAGTTGCTGaaaa  >  1:1051037/1‑140 (MQ=255)                                                                                                                                cggGGGTGGCTTCAAAACCCGAGAAGTTGAAGTCGGTCACTTCGCCGTCTTTTTCCGTCAGCTTAACGTTAATTCGGGTGCGCCCCTGTACAACCTGGAAACGGTTGGCAATGCCCAGCTCGCTGAACAGTTGCTGaaaa  >  1:1357043/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                    CGCAGGCGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCGAGCCAGCTCAGAGAATCAGTCACAAAGCGTTCCCAGTCGGCGGGGGTGACTTCAAAACCCGAGAAGTTGAAGTCGGTCACTTCGCCGTCTTTTTCCGTCAGCTTAACGTTAATTCGGGTGCGCCCCTGTACAACCTGGAAACGGTTGGCAATGCCCAGCTCGCTGAACAGTTGCTGAAAA  >  NC_000913/2261893‑2262159

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑