Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,379,938	C→T	G236S (GGC→AGC)	menF ←	isochorismate synthase MenF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,379,938	0	C	T	100.0%	187.5 / NA	53	G236S (GGC→AGC)	menF	isochorismate synthase MenF
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (30/23);  total (30/23)

GTTTTTATCATCCGCCATCAGCCACTCTCCTAACTGCTGCGCCTGCTTATCATCAGGATTATTTGCTACTGTTCCCGCCAGCGCTTCAGTACGCAGCGCTTTGTCACGCCGCCGCCATAACCGTTCCGGTGAAGAGCCAAGAAAAGCATTTTCGCCATCAAAGGC‑CATGTAAAAATGGTAGCAATTCAGATTCAGTCGACGACTGGCAGCCATCATCGCCGCCGCGTTGACCGGACTTGCGAAATGCAGGTCAGTTGCCCGAGCGAGCACCAC  >  NC_000913/2379801‑2380073                                                                                                                                          |                                                                                                                                         gTTTTTATCATCCGCCATCAGCCACTCTCCTAACTGCTGCGCCTGCTTATCATCAGGATTATTTGCTACTGTTCCCGCCAGCGCTTCAGTACGCAGCGCTTTGTCACGCCGCCGCCATAACCGTTCCGGTGAAGAGCTaa                                                                                                                                        >  2:53382/1‑140 (MQ=255)       atcatcCGCCATCAGCCACTCTCCTAACTGCTGCGCCTGCTTATCATCAGGATTATTTGCTACTGTTCCCGCCAGCGCTTCAGTACGCAGCGCTTTGTCACGCCGCCGCCATAACCGTTCCGGTGAAGAGCTAAGaaact                                                                                                                                  >  1:1343838/1‑138 (MQ=255)            ccGCCATCAGCCACTCTCCTAACTGCTGCGCCTGCTTATCATCAGGATTATTTGCTACTGTTCCCGCCAGCGCTTCAGTACGCAGCGCTTTGTCACGCCGCCGCCATAACCGTTCCGGTGAAGAGCTAAGAAAAGCAttt                                                                                                                             >  2:656452/1‑140 (MQ=255)                         ctctCCTAACTGCTGCGCCTGCTTATCATCAGGATTTTTTGCTACTGTCCCCGCCAGCGCTTCAGTACGCAGCGCTTTGTCACGCCGCCGCCATAACCGTTCCGGTGAAGAGCTAAGAAAAGCATTTTCGCCATCAAAgg                                                                                                                <  1:749165/140‑1 (MQ=255)                         ctccCCTAACTGCTGCGCCTGCTTATCACCAGAATTATTTGCTACTGTCCCCGCCAGCGCTTCAGTACGCAGCGCTTTGTCACGCCGCCGCCATAACCGTTCCGGTGAAGAGCTAAGAAAAGCATTTTCGCCATCAAAgg                                                                                                                <  1:106299/140‑1 (MQ=255)                           ctcCTAACTGCTGCGCCTGCTTATCATCAGGATTATTTGCTACTGTTCCCGCCAGCGCTTCAGTACGCAGCGCTTTGTCACGCCGCCGCCATAACCGTTCCGGTGAAGAGCTAAGAAAAGCATTTTCGCCATCAAAGGc‑c                                                                                                             >  1:673970/1‑140 (MQ=255)                            cccTACCTGCTGCCCCTGTTTATCATCGGGTTTATTTCCTCCTGTCCCCGCCAGCGCTTCAGTACGCAGCGCTTTGTCACGCCGCCGCCATAACCGTTCCGGTGAAGAGCTAAGAAAAGCATTTTCGCCATCAAAGGC‑Ca                                                                                                            <  2:1138853/139‑1 (MQ=255)                                   tgctgcGCCTGCTTATCATCAGGATTATTTGCTACTGTTCCCGCCAGCGCTTCAGTACGCAGCGCTTTGTCACGCCGCCGCCATAACCGTTCCGGTGAAGAGCTAAGAAAAGCATTTTCGCCATCAAAGGC‑CATGTaaaa                                                                                                     >  2:1160494/1‑140 (MQ=255)                                    gctgcGCCTGCTTATCATCAGGATTATTTGCTACTGTTCCCGCCAGCGCTTCAGTACGCAGCGCTTTGTCACGCCGCCGCCATAACCGTTCCGGTGAAGAGCTAAGAAAAGCATTTTCGCCATCAAAGGC‑CATGTaaaaa                                                                                                    >  2:90607/1‑140 (MQ=255)                                    gctgcGCCTGCTTATCATCAGGATTATTTGCTACTGTTCCCGCCAGCGCTTCAGTACGCAGCGCTTTGTCACGCCGCCGCCATAACCGTTCCGGTGAAGAGCTAAGAAAAGCATTTTCGCCATCAAAGGC‑CATGTaaaaa                                                                                                    >  2:410032/1‑140 (MQ=255)                                    gctgcGCCTGCTTATCATCAGGATTATTTGCTACTGTTCCCGCCAGCGCTTCAGTACGCAGCGCTTTGTCACGCCGCCGCCATAACCGTTCCGGTGAAGAGCTAAGAAAAGCATTTTCACCATCAAAGGC‑CATGTaaaaa                                                                                                    >  1:208110/1‑140 (MQ=255)                                     ctgcGCCTGCTTATCATCAGGATTATTTGCTACTGTTCCCGCCAGCGCTTCAGTACGCAGCGCTTTGTCACGCCGCCGCCATAACCGTTCCGGTGAAGAGCTAAGAAAAGCATTTTCGCCATCAAAGGC‑CATGTAAAAAt                                                                                                   >  1:1324813/1‑140 (MQ=255)                                     ctgcGCCTGCTTATCATCAGGATTATTTGCTACTGTTCCCGCCAGCGCTTCAGTACGCAGCGCTTTGTCACGCCGCCGCCATAACCGTTCCGGTGAAGAGCTAAGAAAAGCATTTTCGCCATCAAAGGC‑CATGTAAAAAt                                                                                                   <  1:1390291/140‑1 (MQ=255)                                      tgcGCCTGCTTATCATCAGGATTATTTGCTACTGTTCCCGCCAGCGCTTCAGTACGCAGCGCTTTGTCACGCCGCCGCCATAACCGTTCCGGTGAAGAGCTAAGAAAAGCATTTTCGCCATCAAAGGCTCATGTAAAAAt                                                                                                   >  1:858305/1‑140 (MQ=255)                                      tgcGCCTGCTTATCATCAGGATTATTTGCTACTGTTCCCGCCAGCGCTTCAGTACGCAGCGCTTTGTCACGCCGCCGCCATAACCGTTCCGGTGAAGAGCTAAGAAAAGCATTTTCGCCATCAAAGGC‑CATGTAAAAATg                                                                                                  >  2:753378/1‑140 (MQ=255)                                      tgcGCCTGCTTATCATCAGGATTATTTGCTACTGTTCCCGCCAGCGCTTCAGTACGCAGCGCTTTGTCACGCCGCCGCCATAACCGTTCCGGTGAAGAGCTAAGAAAAGCATTTTCGCCATCAAAGGC‑CATGTAAAAATg                                                                                                  >  1:479435/1‑140 (MQ=255)                                        cgcCTGTTTATCATCAGGTTTTTTTGCTCCTGTCCCCGCCAGCGCTTCAGTACGCAGCGCTTTGTCACGCCGCCGCCATAACCGTTCCGGTGAAGAGCTAAGAAAAGCATTTTCGCCATCAAAGGC‑CATGTAAAAATGGt                                                                                                <  2:908667/140‑1 (MQ=255)                                        cgcCTGCTTATCATCAGGATTATTTGCTACTGTTCCCGCCAGCGCTTCAGTACGCAGCGCTTTGTCACGCCGCCGCCATAACCGTTCCGGTGAAGAGCTAAGAAAAGCATTTTCGCCATCAAAGGC‑CATGTAAAAATGGt                                                                                                >  1:309307/1‑140 (MQ=255)                                          ccTGTTTATCATAAAGATTATTGCCTACTGTCCCCGCCAGCGCTTCAGTACGCAGCGCTTTGTCACGCCGCCGCCATAACCGTTCCGGTGAAGAGCTAAGAAAAGCATTTTCGCCATCAAAGGC‑CATGTAAAAATGGTAg                                                                                              <  2:785466/140‑1 (MQ=255)                                          ccTGTTTATCACCAGGTTTTTTTGCTATTGTCCCCGCCAGCGCTTCAGTACGCAGCGCTTTGTCACGCCGCCGCCATAACCGTTCCGGTGAAGAGCTAAGAAAAGCATTTTCGCCATCAAAGGC‑CATGTAAAAATGGTAg                                                                                              <  2:1324813/140‑1 (MQ=255)                                          ccTGCTTATCATCAGGATTATTTGCTCCTGTCCCCGCCAGCGCTTCAGTACGCAGCGCTTTGTCACGCCGCCGCCATAACCGTTCCGGTGAAGAGCTAAGAAAAGCATTTTCGCCATCAAAGGC‑CATGTAAAAATGGTAg                                                                                              <  1:253923/140‑1 (MQ=255)                                          ccTGCTTATCATCAAGATTATTTGCTACTGTTCCCGCCAGCGCTTCAGTACGCAGCGCTTTGTCACGCCGCCGCCATAACCGTTCCGGTGAAGAGCTAAGAAAAGCATTTTCGCCATCAAAGGC‑CATGTAAAAATGGTAg                                                                                              >  1:785466/1‑140 (MQ=255)                                           cTGTTTACCATCAGGTTTTTTTGCTCCTGTCCCCGCCAGCGCTTCAGTACGCAGCGCTTTGTCACGCCGCCGCCATAACCGTTCCGGTGAAGAGCTAAGAAAAGCATTTTCGCCATCAAAGGC‑CATGTAAAAATGGTAGc                                                                                             <  2:1201163/140‑1 (MQ=255)                                              tttATCATCAGTTTTTTTTGCTCGTGTTCCCGCCAGCGCTTCAGTACGCAGCGCTTTGTCACGCCGCCGCCATAACCGTTCCGGTGAAGAGCTAAGAAAAGCATTTTCGCCATCAAAGGC‑CATGTAAAAATGGTAGCAat                                                                                          <  2:1307668/139‑1 (MQ=255)                                              cTTATCATCAGGATTATTTGCTACTGTTCCCGCCAGCGCTTCAGTACGCAGCGCTTTGTCACGCCGCCGCCATAACCGTTCCGGTGAAGAGCTAAGAAAAGCATTTTCGCCATCAAAGGC‑CATGTAAAAATGGTAGCAat                                                                                          <  1:656452/140‑1 (MQ=255)                                                     tcaGGTTTTTTTGCTACTGTCCCCGCCAGCGCTTCAGTACGCAGCGCTTTGTCACGCCGCCGCCATAACCGTTCCGGTGAAGAGCTAAGAAAAGCATTTTCGCCATCAAAGGC‑CATGTAAAAATGGTAGCAattcagatt                                                                                   <  1:53019/140‑1 (MQ=255)                                                        ggATTATTTGCTACTGTTCCCGCCAGCGCTTCAGTACGCAGCGCTTTGTCACGCCGCCGCCATAACCGTTCCGGTGAAGAGCTAAGAAAAGCATTTTCGCCATCAAAGGC‑CATGTAAAAATGGTAGCAattcagattcag                                                                                >  1:1074478/1‑140 (MQ=255)                                                          tttttttGCTACTGTCCCCGCCAGCGCTTCAGTACGCAGCGCTTTGTCACGCCGCCGCCATAACCGTTCCGGTGAAGAGCTAAGAAAAGCATTTTCGCCATCAAAGGC‑CATGTAAAAATGGTAGCAATTCAGATTCAGTc                                                                              <  2:654546/136‑1 (MQ=255)                                                                   tACTGTTCCCGCCAGCGCTTCAGTACGCAGCGCTTTGTCACGCCGCCGCCATAACCGTTCCGGTGAAGAGCTAAGAAAAGCATTTTCGCCATCAAAGGC‑CATGTAAAAATGGTAGCAATTCAGATTCAGTCGACGACTgg                                                                     >  2:358593/1‑140 (MQ=255)                                                                   tACTGTTCCCGCCAGCGCTTCAGTACGCAGCGCTTTGTCACGCCGCCGCCATAACCGTTCCGGTGAAGAGCTAAGAAAAGCATTTTCGCCATCAAAGGC‑CATGTAAAAATGGTAGCAATTCAGATTCAGTCGACGACTgg                                                                     <  2:1007260/140‑1 (MQ=255)                                                                   tACTGTTCCCGCCAGCGCTTCAGTACGCAGCGCTTTGTCACGCCGCCGCCATAACCGTTCCGGTGAAGAGCTAAGAAAAGCATTTTCGCCATCAAAGGC‑CATGTAAAAATGGTAGCAATTCAGATTCAGTCGACGACTgg                                                                     >  1:451262/1‑140 (MQ=255)                                                                        ttCCCGCCAGCGCTTCAGTACGCAGCGCTTTGTCACGCCGCCGCCATAACCGTTCCGGTGAAGAGCTAAGAAAAGCATTTTCGCCATCAAAGGC‑CATGTAAAAATGGTAGCAATTCAGATTCAGTCGACGACTGGCAGcc                                                                >  1:197400/1‑140 (MQ=255)                                                                            cGCCAGCGCTTCAGTACGCAGCGCTTTGTCACGCCGCCGCCATAACCGTTCCGGTGAAGAGCTAAGAAAAGCATTTTCGCCATCAAAGGC‑CATGTAAAAATGGTAGCAATTCAGATTCAGTCGACGACTGGCAGCcatca                                                            >  2:544015/1‑140 (MQ=255)                                                                               cAGCGCTTCAGTACGCAGCGCTTTGTCACGCCGCCGCCATAACCGTTCCGGTGAAGAGCTAAGAAAAGCATTTTCGCCATCAAAGGC‑CATGTAAAAATGGTAGCAATTCAGATTCAGTCGACGACTGGCAGCCATCATcg                                                         <  1:1016972/140‑1 (MQ=255)                                                                                  cgcTTCAGTACGCAGCGCTTTGTCACGCCGCCGCCATAACCGTTCCGGTGAAGAGCTAAGAAAAGCATTTTCGCCATCAAAGGC‑CATGTAAAAATGGTAGCAATTCAGATTCAGTCGACGACTGGCAGCCATCATcgccg                                                      >  2:208768/1‑140 (MQ=255)                                                                                  cgcTTCAGTACGCAGCGCTTTGTCACGCCGCCGCCATAACCGTTCCGGTGAAGAGCTAAGAAAAGCATTTTCGCCATCAAAGGC‑CATGTAAAAATGGTAGCAATTCAGATTCAGTCGACGACTGGCAGCCATCATcgccg                                                      >  1:664282/1‑140 (MQ=255)                                                                                  cgcTTCAGTACGCAGCGCTTTGTCACGCCGCCGCCATAACCGTTCCGGTGAAGAGCTAAGAAAAGCATTTTCGCCATCAAAGGC‑CATGTAAAAATGGTAGCAATTCAGATTCAGTCGACGACTGGCAGCCATCATcgccg                                                      >  2:660260/1‑140 (MQ=255)                                                                                    cTTCAGTACGCAGCGCTTTGTCACGCCGCCGCCATAACCGTTCCGGTGAAGAGCTAAGAAAAGCATTTTCGCCATCAAAGGC‑CATGTAAAAATGGTAGCAATTCAGATTCAGTCGACGACTGGCAGCCATCATcgccgcc                                                    >  2:268965/1‑140 (MQ=255)                                                                                         gTACGCAGCGCTTTGTCACGCCGCCGCCATAACCGTTCCGGTGAAGAGCTAAGAAAAGCATTTTCGCCATCAAAGGC‑CATGTAAAAATGGTAGCAATTCAGATTCAGTCGACGACTGGCAGCCATCATCGCCGCCGCGtt                                               >  2:553817/1‑140 (MQ=255)                                                                                            cGCAGCGCTTTGTCACGCCGCCGCCATAACCGTTCCGGTGAAGAGCTAAGAAAAGCATTTTCGCCATCAAAGGC‑CATGTAAAAATGGTAGCAATTCAGATTCAGTCGACGACTGGCAGCCATCATCGCCGCCGCGTTGAc                                            <  1:90607/140‑1 (MQ=255)                                                                                                 cgcTTTGTCACGCCGCCGCCATAACCGTTCCGGTGAAGAGCTAAGAAAAGCATTTTCGCCATCAAAGGC‑CATGTAAAAATGGTAGCAATTCAGATTCAGTCGACGACTGGCAGCCATCATCGCCGCCGCGTTGACCGGAc                                       >  2:1147311/1‑140 (MQ=255)                                                                                                    tttGTCACGCCGCCGCCATAACCGTTCCGGTGAAGAGCTAAGAAAAGCATTTTCGCCATCAAAGGC‑CATGTAAAAATGGTAGCAATTCAGATTCAGTCGACGACTGGCAGCCATCATCGCCGCCGCGTTGACCGGACTTg                                    >  1:743154/1‑140 (MQ=255)                                                                                                    tttGTCACGCCGCCGCCATAACCGTTCCGGTGAAGAGCTAAGAAAAGCATTTTCGCCATCAAAGGC‑CATGTAAAAATGGTAGCAATTCAGATTCAGTCGACGACTGGCAGCCATCATCGCCGCCGCGTTGACCGGACTTg                                    >  2:61599/1‑140 (MQ=255)                                                                                                      tGTCACGCCGCCGCCATAACCGTTCCGGTGAAGAGCTAAGAAAAGCATTTTCGCCATCAAAGGC‑CATGTAAAAATGGTAGCAATTCAGATTCAGTCGACGACTGGCAGCCATCATCGCCGCCGCGTTGACCGGACTTGCg                                  <  2:841975/140‑1 (MQ=255)                                                                                                              cgccgccATAACCGTTCCGGTGAAGAGCTAAGAAAAGCATTTTCGCCATCAAAGGC‑CATGTAAAAATGGTAGCAATTCAGATTCAGTCGACGACTGGCAGCCATCATCGCCGCCGCGTTGACCGGACTTGCGAAATGCAg                          >  2:468160/1‑140 (MQ=255)                                                                                                                 cgccATAACCGTTCCGGTGAAGAGCTAAGAAAAGCATTTTCGCCATCAAAGGC‑CATGTAAAAATGGTAGCAATTCAGATTCAGTCGACGACTGGCAGCCATCATCGCCGCCGCGTTGACCGGACTTGCGAAATGCAGGTc                       <  1:1147311/140‑1 (MQ=255)                                                                                                                        aCCGTTCCGGTGAAGAGCTAAGAAAAGCATTTTCGCCATCAAAGGC‑CATGTAAAAATGGTAGCAATTCAGATTCAGTCGACGACTGGCAGCCATCATCGCCGCCGCGTTGACCGGACTTGCGAAATGCAGGTCAGTTGcc                <  1:523605/140‑1 (MQ=255)                                                                                                                         ccGTTCCGGTGAAGAGCTAAGAAAAGCATTTTCGCCATCAAAGGC‑CATGTAAAAATGGTAGCAATTCAGATTCAGTCGACGACTGGCAGCCATCATCGCCGCCGCGTTGACCGGACTTGCGAAATGCAGGTCAGTTGccc               <  2:666271/140‑1 (MQ=255)                                                                                                                            ttCCGGTGAAGAGCTAAGAAAAGCATTTTCGCCATCAAAGGC‑CATGTAAAAATGGTAGCAATTCAGATTCAGTCGACGACTGGCAGCCATCATCGCCGCCGCGTTGACCGGACTTGCGAAATGCAGGTCAGTTGCCcgag            >  1:336748/1‑140 (MQ=255)                                                                                                                            ttCCGATGAAGGGCTAAGAAGTGCTTTTACGCCACCAAATGC‑TATGTAAATATCATCGCAATTCAGACTTCGTCGTGGACTTGCAGTCCGCGCCGCAGCCGCGTAGACCGGACTTGCGAAATGCAGGTCTGTTGCTcgtg            <  2:13267/140‑3 (MQ=255)                                                                                                                             tCCGGTGAAGAGCTAAGAAAAGCATTTTCGCCATCAAAGGC‑CATGTAAAAATGGTAGCAATTCAGATTCAGTCGACGACTGGCAGCCATCATCGCCGCCGCGTTGACCGGACTTGCGAAATGCAGGTCAGTTGCCcgagc           >  1:466288/1‑140 (MQ=255)                                                                                                                                ggTGAAGAGCTAAGAAAAGCATTTTCGCCATCAAAGGC‑CATGTAAAAATGGTAGCAATTCAGATTCAGTCGACGACTGGCAGCCATCATCGCCGCCGCGTTGACCGGACTTGCGAAATGCAGGTCAGTTGCCcgagcgag        <  2:197400/140‑1 (MQ=255)                                                                                                                                  tGAAGAGCTAAGAAAAGCATTTTCGCCATCAAAGGC‑CATGTAAAAATGGTAGCAATTCAGATTCAGTCGACGACTGGCAGCCATCATCGCCGCCGCGTTGACCGGACTTGCGAAATGCAGGTCAGTTGCCCGAGCGAGca      <  1:660260/140‑1 (MQ=255)                                                                                                                                      gagCTAAGAAAAGCATTTTCGCCATCAAAGGC‑CATGTAAAAATGGTAGCAATTCAGATTCAGTCGACGACTGGCAGCCATCATCGCCGCCGCGTTGACCGGACTTGCGAAATGCAGGTCAGTTGCCCGAGCGAGcaccac  <  2:1376574/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                         GTTTTTATCATCCGCCATCAGCCACTCTCCTAACTGCTGCGCCTGCTTATCATCAGGATTATTTGCTACTGTTCCCGCCAGCGCTTCAGTACGCAGCGCTTTGTCACGCCGCCGCCATAACCGTTCCGGTGAAGAGCCAAGAAAAGCATTTTCGCCATCAAAGGC‑CATGTAAAAATGGTAGCAATTCAGATTCAGTCGACGACTGGCAGCCATCATCGCCGCCGCGTTGACCGGACTTGCGAAATGCAGGTCAGTTGCCCGAGCGAGCACCAC  >  NC_000913/2379801‑2380073

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑