Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,510,402	T→C	V258A (GTG→GCG)	yfeO →	putative transport protein YfeO

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,510,402	0	T	C	100.0%	201.3 / NA	58	V258A (GTG→GCG)	yfeO	putative transport protein YfeO
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (27/31);  total (27/31)

TGCTCATTACGGACAGATGGAAATGACCGATATTCTCAGCGGTGCAATTGTCGCGGCGATTGCCATCGCAGCAGGGATGGTCGCCGTATGGTGCTTACCACGGTTGCACGCGATGATGCATCAAATGAAAAATCCGGTGCTCGTGCTGGGTATTGGCGGATTTATTCTCGGTATTCTGGGGGTTATTGGTGGACCAGTTTCGCTGTTTAAAGGGCTGGATGAGATGCAGCAGATGGTGGCAAATCAGGCTTTCAGCACCAGCG  >  NC_000913/2510265‑2510527                                                                                                                                          |                                                                                                                              tGCTCATTACGGACAGATGGAAATGACCGATATTCTCAGCGGTGCAATTGTCGCGGCGATTGCCATCGCAGCAGGGATGGTCGCCGTATGGTGCTTACCACGGTTGCACGCGATGATGCATCAAATGAAAAATCCGgcgc                                                                                                                             >  1:51017/1‑140 (MQ=255)    tCATTACGGACAGATGGAAATGACCGATATTCTCAGCGGTGCAATTGTCGCGGCGATTGCCATCGCAGCAGGGATGGTCGCCGTATGGTGCTTACCACGGTTGCACGCGATGATGCATCAAATGAAAAATCCGGCGCTCg                                                                                                                          >  1:1377202/1‑140 (MQ=255)        tACGGACAGATGGAAATGACCGATATTCTCAGCGGTGCAATTGTCGCGGCGATTGCCATCGCAGCAGGGATGGTCGCCGTATGGTGCTTACCACGGTTGCACGCGATGATGCATCAAATGAAAAATCCGGCGCTCGTGCt                                                                                                                      <  1:655153/140‑1 (MQ=255)                gATGGAAATGACCGATATTCTCAGCGGTGCAATTGTCGCGGCGATTGCCATCGCAGCAGGGATGGTCGCCGTATGGTGCTTACCACGGTTGCACGCGATGATGCATCAAATGAAAAATCCGGCGCTCGTGCTGGGTATTg                                                                                                              >  1:1309212/1‑140 (MQ=255)                 aTGGAAATGACCGATATTCTCAGCGGTGCAATTGTCGCGGTGATTGCCATCGCAGCAGGGATGGTCGCCGTATGGTGCTTACCACGGTTGCACGCGATGATGCATCAAATGAAAAATCCGGCGCTCGTGCTGGGTATTgg                                                                                                             <  1:1187262/140‑1 (MQ=255)                 aTGGAAATGACCGATATTCTCAGCGGTGCAATTGTCGCGGCGATTGCCATCGCAGCAGGGATGGTCGCCGTATGGTGCTTACCACGGTTGCACGCGATGGTGCATCAAATGAAAAATCCGGCGCTCGTGCTGGGTATTgg                                                                                                             <  1:918890/140‑1 (MQ=255)                 aTGGAAATGACCGATATTCTCAGCGGTGCAATTGTCGCGGCGATTGCCATCGCAGCAGGGATGGTCGCCGTATGGTGCTTACCACGGTTGCACGCGATGATGCATCAAATGAAAAATCCGGCGCTCGTGCTGGGTATTgg                                                                                                             >  1:472246/1‑140 (MQ=255)                 aTGGAAATGACCGATATTCTCAGCGGTGCAATTGTCGCGGCGATTGCCATCGCAGCAGGGATGGTCGCCGTATGGTGCTTACCACGGTTGCACGCGATGATGCATCAAATGAAAAATCCGGCGCTCGTGCTGGGTATTgg                                                                                                             <  2:1094962/140‑1 (MQ=255)                 aTGGAAATGACCGATATTCTCAGCGGTGCAATTGTCGCGGCGATTGCCATCGCAGCAGGGATGGTCGCCGTATGGTGCTTACCACGGTTGCACGCGATGATGCATCAAATGAAAAATCCGGCGCTCGTGCTGGGTATTgg                                                                                                             >  2:1209031/1‑140 (MQ=255)                 aTGGAAATGACCGATATTCTCAGCGGTGCAATTGTCGCGGCGATTGCCATCGCAGCAGGGATGGTCGCCGTATGGTGCTTACCACGGTTGCACGCGATGATGCATCAAATGAAAAATCCGGCGCTCGTGCTGGGTATTgg                                                                                                             >  1:441929/1‑140 (MQ=255)                  tGGAAATGACCGATATTCTCAGCGGTGCAATTGTCGCGGCGATTGCCATCGCAGCAGGGATGGTCGCCGTATGGTGCTTACCACGGTTGCACGCGATGATGCATCAAATGAAAAATCCGGCGCTCGTGCTGGGTATTGGc                                                                                                            >  2:172747/1‑140 (MQ=255)                      aaTGACCGATATTCTCAGCGGTGCAATTGTCGCGGCGATTGCCATCGCAGCAGGGATGGTCGCCGTATGGTGCTTACCACGGTTGCACGCGATGATGCATCAAATGAAAAATCCGGCGCTCGTGCTGGGTATTGGCGGAt                                                                                                        >  1:213789/1‑140 (MQ=255)                      aaTGACCGATATTCTCAGCGGTGCAATTGTCGCGGCGATTGCCATCGCAGCAGGGATGGTCGCCGTATGGTGCTTACCACGGTTGCACGCGATGATGCATCAAATGAAAAATCCGGCGCTCGTGCTGGGTATTGGCGGAt                                                                                                        <  2:472246/140‑1 (MQ=255)                        tGACCGATATTCTCAGCGGTGCAATTGTCGCGGCGATTGCCATCGCAGCAGGGATGGTCGCCGTATGGTGCTTACCACGGTTGCACGCGATGATGCATCAAATGAAAAATCCGGCGCTCGTGCTGGGTATTGGCGGAttt                                                                                                      <  2:575437/140‑1 (MQ=255)                          aCCGATATTCTCAGCGGTGCAATTGTCGCGGCGATTGCCATCGCAGCAGGGATGGTCGCCGTATGGTGCTTACCACGGTTGCACGCGATGATGCATCAAATGAAAAATCCGGCGCTCGTGCTGGGTATTGGCGGATTTAt                                                                                                    <  2:1377202/140‑1 (MQ=255)                           ccGATATTCTCAGCGGTGCAATTGTCGCGGCGATTGCCATCGCAGCAGGGATGGTCGCCGTATGGTGCTTACCACGGTTGCACGCGATGATGCATCAAATGAAAAATCCGGCGCTCGTGCTGGGTATTGGCGGATTTAtt                                                                                                   <  1:496324/140‑1 (MQ=255)                           ccGATATTCTCAGCGGTGCAATTGTCGCGGCGATTGCCATCGCAGCAGGGATGGTCGCCGTATGGTGCTTACCACGGTTGCACGCGATGATGCATCAAATGAAAAATCCGGCGCTCGTGCTGGGTATTGGCGGATTTAtt                                                                                                   <  1:740635/140‑1 (MQ=255)                           ccGATATTCTCAGCGGTGCAATTGTCGCGGCGATTGCCATCGCAGCAGAGATGGTCGCCGTATGGTGCTTACCACGGTTGCACGCGATGATGCATCAAATGAAAAATCCGGCGCTCGTGCTGGGTATTGGCGGATTTAtt                                                                                                   >  2:1220047/1‑140 (MQ=255)                              atatTCTCAGCGGTGCAATTGTCGCGGCGATTGCCATCGCAGCAGGGATGGTCGCCGTATGGTGCTTACCACGGTTGCACGCGATGATGCATCAAATGAAAAATCCGGCGCTCGTGCTGGGTATTGGCGGATTTATtctc                                                                                                <  2:329530/140‑1 (MQ=255)                              atatTCTCAGCGGTGCAATTGTCGCGGCGATTGCCATCGCAGCAGGGATGGTCGCCGTATGGTGCTTACCACGGTTGCACGCGATGATGCATCAAATGAAAAATCCGGCGCTCGTGCTGGGTATTGGCGGATTTATtctc                                                                                                <  2:51017/140‑1 (MQ=255)                                        cGGTGCAATTGTCGCGGCGATTGCCATCGCAGCAGGGATGGTCGCCGTATGGTGCTTACCACGGTTGCACGCGATGATGCATCAAATGAAAAATCCGGCGCTCGTGCTGGGTATTGGCGGATTTATTCTCGGTATTCTgg                                                                                      <  1:678493/140‑1 (MQ=255)                                        cGGTGCAATTGTCGCGGCGATTGCCATCGCAGCAGGGATGGTCGCCGTATGGTGCTTACCACGGTTGCACGCGATAATGCATCAAATGAAAAATCCGGCGCTCGTGCTGGGTATTGGCGGATTTATTCTCGGTATTCTgg                                                                                      <  1:338120/140‑1 (MQ=255)                                           tGCAATTGTCGCGGCGATTGCCATCGCAGCAGGGATGGTCGCCGTATGGTGCTTACCACGGTTGCACGCGATGATGCATCAAATGAAAAATCCGGCGCTCGTGCTGGGTATTGGCGGATTTATTCTCGGTATTCTggggg                                                                                   >  2:924274/1‑140 (MQ=255)                                           tGCAATTGTCGCGGCGATTGCCATCGCAGCAGGGATGGTCGCCGTATGGTGCTTACCACGGTTGCACGCGATGATGCATCAAATGAAAAATCCGGCGCTCGTGCTGGGTATTGGCGGATTTATTCTCGGTATTCTggggg                                                                                   >  2:1070243/1‑140 (MQ=255)                                           tGCAATTGTCGCGGCGATTGCCATCGCAGCAGGGATGGTCGCCGTATGGTGCTTACCACGGTTGCACGCGATGATGCATCAAATGAAAAATCCGGCGCTCGTGCTGGGTATTGGCGGATTTATTCTCGGTATTCTggggg                                                                                   >  1:80824/1‑140 (MQ=255)                                           tGCAATTGTCGCGGCGATTGCCATCGCAGCAGGGATGGTCGCCGTATGGTGCTTACCACGGTTGCACGCGATGATGCATCAAATGAAAAATCCGGCGCTCGTGCTGGGTATTGGCGGATTTATTCTCGGTATTCTggggg                                                                                   >  2:1280657/1‑140 (MQ=255)                                            gCAATTGTCGCGGCGATTGCCATCGCAGCAGGGATGGTCGCCGTATGGTGCTTACCACGGTTGCACGCGATGATGCATCAAATGAAAAATCCGGCGCTCGTGCTGGGTATTGGCGGATTTATTCTCGGTATTCTGGGGGt                                                                                  <  2:1309212/140‑1 (MQ=255)                                            gCAATTGTCGCGGCGATTGCCATCGCAGCAGGGATGGTCGCCGTATGGTGCTTACCACGGTTGCACGCGATGATGCATCAAATGAAAAATCCGGCGCTCGTGCTGGGTATTGGCGGATTTATTCTCGGTATTCTGGGGGt                                                                                  <  2:21764/140‑1 (MQ=255)                                            gCAATTGTCGCGGCGATTGCCATCGCAGCAGGGATGGTCGCCGTATGGTGCTTACCACGGTTGCACGCGATGATGCATCAAATGAAAAATCCGGCGCTCGTGCTGGGTATTGGCGGATTTATTCTCGGTATTCTGGGGGt                                                                                  <  1:172747/140‑1 (MQ=255)                                                  gTCGCGGCGATTGCCATCGCAGCAGGGATGGTCGCCGTATGGTGCTTACCACGGTTGCACGCGATGATGCATCAAATGAAAAATCCGGCGCTCGTGCTGGGTATTGGCGGATTTATTCTCGGTATTCTGGGGGTTATtgg                                                                            <  1:882533/140‑1 (MQ=255)                                                       ggcgATTGCCATCGCAGCAGGGATGGTCGCCGTATGGTGCTTACCACGGTTGCACGCGATGATGCATCAAATGAAAAATCCGGCGCTCGTGCTGGGTATTGGCGGATTTATTCTCGGTATTCTGGGGGTTATTGGTGGAc                                                                       <  2:80824/140‑1 (MQ=255)                                                                 aTCGCAGCAGGGATGGTCGCCGTATGGTGCTTACCACGGTTGCACGCGATGATGCATCAAATGAAAAATCCGGCGCTCGTGCTGGGTATTGGCGGATTTATTCTCGGTATTCTGGGGGTTATTGGTGGACCAGTTTCGCt                                                             <  2:464990/140‑1 (MQ=255)                                                                                gTCGCCGTATGGTGCTTACCACGGTTGCACGCGATGATGCATCAAATGAAAAATCCGGCGCTCGTGCTGGGTATTGGCGGATTTATTCTCGGTATTCTGGGGGTTATTGGTGGACCAGTTTCGCTGTTTAAAGGGCTGGa                                              <  1:1070243/140‑1 (MQ=255)                                                                                gTCGCCGTATGGTGCTTACCACGGTTGCACGCGATGATGCATCAAATGAAAAATCCGGCGCTCGTGCTGGGTATTGGCGGATTTATTCTCGGTATTCTGGGGGTTATTGGTGGACCAGTTTCGCTGTTTAAAGGGCTGGa                                              <  1:908238/140‑1 (MQ=255)                                                                                  cGCCGTATGGTGCTTACCACGGTTGCACGCGATGATGCATCAAATGAAAAATCCGGCGCTCGTGCTGGGTATTGGCGGATTTATTCTCGGTATTCTGGGGGTTATTGGTGGACCAGTTTCGCTGTTTAAAGGGCTGGATg                                            <  1:1220047/140‑1 (MQ=255)                                                                                    ccGTATGGTGCTTACCACGGTTGCACGCGATGATGCATCAAATGAAAAATCCGGCGCTCGTGCTGGGTATTGGCGGATTTATTCTCGGTATTCTGGGGGTTATTGGTGGACCAGTTTCGCTGTTTAAAGGGCTGGa                                              <  2:646976/136‑1 (MQ=255)                                                                                    ccGTATGGTGCTTACCACGGTTGCACGCGATGATGCATCAAATGAAAAATCCGGCGCTCGTGCTGGGTATTGGCGGATTTATTCTCGGTATTCTGGGGGTTATTGGTGGACCAGTTTCGCTGTTTAAAGGGCTGGa                                              >  1:646976/1‑136 (MQ=255)                                                                                     cGTATGGTGCTTACCACGGTTGCACGCGATGATGCATCAAATGAAAAATCCGGCGCTCGTGCTGGGTATTGGCGGATTTATTCTCGGTATTCTGGGGGTTATTGGTGGACCAGTTTCGCTGTTTAAAGGGCTGGATgaga                                         >  2:1197071/1‑140 (MQ=255)                                                                                       tATGGTGCTTACCACGGTTGCACGCGATGATGCATCAAATGAAAAATCCGGCGCTCGTGCTGGGTATTGGCGGATTTATTCTCGGTATTCTGGGGGTTATTGGTGGACCAGTTTCGCTGTTTAAAGGGCTGGATGAGATg                                       >  2:376610/1‑140 (MQ=255)                                                                                          ggTGCTTACCACGGTTGCACGCGATGATGCATCAAATGAAAAATCCGGCGCTCGTGCTGGGTATTGGCGGATTTATTCTCGGTATTCTGGGGGTTTTTGGTGGACCAGTTTCGCTGTTTAAAGGGCTGGATGAGATgcag                                    >  2:581101/1‑140 (MQ=255)                                                                                            tGCTTACCACGGTTGCACGCGATGATGCATCAAATGAAAAATCCGGCGCTCGTGCTGGGTATTGGCGGATTTATTCTCGGTATTCTGGGGGTTATTGGTGGACCAGTTTCGCTGTTTAAAGGGCTGGATGAGATgcagca                                  <  1:924274/140‑1 (MQ=255)                                                                                              cTTACCACGGTTGCACGCGATGATGCATCAAATGAAAAATCCGGCGCTCGTGCTGGGTATTGGCGGATTTATTCTCGGTATTCTGGGGGTTATTGGTGGACCAGTTTCGCTGTTTAAAGGGCTGGATGAGATGCAGCAGa                                >  2:272219/1‑140 (MQ=255)                                                                                                 aCCACGGTTGCACGCGATGATGCATCAAATGAAAAATCCGGCGCTCGTGCTGGGTATTGGCGGATTTATTCTCGGTATTCTGGGGGTTATTGGTGGACCAGTTTCGCTGTTTAAAGGGCTGGATGAGATGCAGCAGAtgg                             >  1:1191064/1‑140 (MQ=255)                                                                                                 aCCACGGTTGCACGCGATGATGCATCAAATGAAAAATCCGGCGCTCGTGCTGGGTATTGGCGGATTTATTCTCGGTATTCTGGGGGTTATTGGTGGACCAGTTTCGCTGTTTAAAGGGCTGGATGAGATGCAGCAGAtgg                             >  1:75636/1‑140 (MQ=255)                                                                                                      ggTTGCACGCGATGATGCATCAAATGAAAAATCCGGCGCTCGTGCTGGGTATTGGCGGATTTATTCTCGGTATTCTGGGGGTTATTGGTGGACCAGTTTCGCTGTTTAAAGGGCTGGATGAGAt                                        >  1:1153627/1‑124 (MQ=255)                                                                                                      ggTTGCACGCGATGATGCATCAAATGAAAAATCCGGCGCTCGTGCTGGGTATTGGCGGATTTATTCTCGGTATTCTGGGGGTTATTGGTGGACCAGTTTCGCTGTTTAAAGGGCTGGATGAGAt                                        <  2:1153627/124‑1 (MQ=255)                                                                                                      ggTTGCACGCGATGATGCATCAAATGAAAAATCCGGCGCTCGTGCTGGGTATTGGCGGATTTATTCTCGGTATTCTGGGGGTTATTGGTGGACCAGTTTCGCTGTTTAAAGGGCTGGATGAGATGCAGCAGAtggtg                           <  1:542908/137‑1 (MQ=255)                                                                                                      ggTTGCACGCGATGATGCATCAAATGAAAAATCCGGCGCTCGTGCTGGGTATTGGCGGATTTATTCTCGGTATTCTGGGGGTTATTGGTGGACCAGTTTCGCTGTTTAAAGGGCTGGATGAGATGCAGCAGAtggtg                           >  2:542908/1‑137 (MQ=255)                                                                                                      ggTTGCACGCGATGATGCATCAAATGAAAAATCCGGCGCTCGTGCTGGGTATTGGCGGATTTATTCTCGGTATTCTGGGGGTTATTGGTGGACCAGTTTCGCTGTTTAAAGGGCTGGATGAGATGCAGCAGATGGTGGCa                        >  2:365142/1‑140 (MQ=255)                                                                                                      ggTTGCACGCGATGATGCATCAAATGAAAAATCCGGCGCTCGTGCTGGGTATTGGCGGATTTATTCTCGGTATTCTGGGGGTTATTGGTGGACCAGTTTCGCTGTTTAAAGGGCTGGATGAGATGCAGCAGATGGTGGCa                        >  1:80437/1‑140 (MQ=255)                                                                                                        ttGCACGCGATGATGCATCAAATGAAAAATCCGGCGCTCGTGCTGGGTATTGGCGGATTTATTCTCGGTATTCTGGGGGTTATTGGTGGACCAGTTTCGCTGTTTAAAGGGCTGGATGAGATGCAGCAGATGGTGGCaaa                      >  1:837848/1‑140 (MQ=255)                                                                                                         tGCACGCGATGATGCATCAAATGAAAAATCCGGCGCTCGTGCTGGGTATTGGCGGATTTATTCTCGGTATTCTGGGGGTTATTGGTGGACCAGTTTCGCTGTTTAAAGGGCTGGATGAGATGCAGCAGATGGTGGCAAAt                     <  2:80437/140‑1 (MQ=255)                                                                                                           cACGCGATGATGCATCAAATGAAAAATCCGGCGCTCGTGCTGGGTATTGGCGGATTTATTCTCGGTATTCTGGGGGTTATTGGTGGACCAGTTTCGCTGTTTAAAGGGCTGGATGAGATGCAGCAGATGGTGGCAAATCa                   >  1:842293/1‑140 (MQ=255)                                                                                                            aCGCGATGATGCATCAAATGAAAAATCCGGCGCTCGTGCTGGGTATTGGCGGATTTATTCTCGGTATTCTGGGGGTTATTGGTGGACCAGTTTCGCTGTTTAAAGGGCTGGATGAGATGCAGCAGATGGTGGCAAATCAg                  <  1:1209031/140‑1 (MQ=255)                                                                                                            aCGCGATGATGCATCAAATGAAAAATCCGGCGCTCGTGCTGGGTATTGGCGGATTTATTCTCGGTATTCTGGGGGTTATTGGTGGACCAGTTTCGCTGTTTAAAGGGCTGGATGAGATGCAGCAGATGGTGGCAAATCAg                  <  1:1149772/140‑1 (MQ=255)                                                                                                            aCGCGATGATGCATCAAATGAAAAATCCGGCGCTCGTGCTGGGTATTGGCGGATTTATTCTCGGTATTCTGGGGGTTATTGGTGGACCAGTTTCGCTGTTTAAAGGGCTGGATGAGATGCAGCAGATGGTGGCAAATCAg                  <  1:1190148/140‑1 (MQ=255)                                                                                                               cgATGATGCATCAAATGAAAAATCCGGCGCTCGTGCTGGGTATTGGCGGATTTATTCTCGGTATTCTGGGGGTTATTGGTGGACCAGTTTCGCTGTTTAAAGGGCTGGATGAGATGCAGCAGATGGTGGCAAATCAGGCt               >  2:691080/1‑140 (MQ=255)                                                                                                                 atgatgCATCAAATGAAAAATCCGGCGCTCGTGCTGGGTATTGGCGGATTTATTCTCGGTATTCTGGGGGTTATTGGTGGACCAGTTTCGCTGTTTAAAGGGCTGGATGAGATGCAGCAGATGGTGGCAAATCAGGCttt             <  1:691080/140‑1 (MQ=255)                                                                                                                            aaTGAAAAATCCGGCGCTCGTGCTGGGTATTGGCGGATTTATTCTCGGTATTCTGGGGGTTATTGGTGGACCAGTTTCGCTGTTTAAAGGGCTGGATGAGATGCAGCAGATGGTGGCAAATCAGGCTTTCAGCACCAGCg  >  1:1033664/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                              TGCTCATTACGGACAGATGGAAATGACCGATATTCTCAGCGGTGCAATTGTCGCGGCGATTGCCATCGCAGCAGGGATGGTCGCCGTATGGTGCTTACCACGGTTGCACGCGATGATGCATCAAATGAAAAATCCGGTGCTCGTGCTGGGTATTGGCGGATTTATTCTCGGTATTCTGGGGGTTATTGGTGGACCAGTTTCGCTGTTTAAAGGGCTGGATGAGATGCAGCAGATGGTGGCAAATCAGGCTTTCAGCACCAGCG  >  NC_000913/2510265‑2510527

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑