Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,639,288	T→C	intergenic (‑5/+13)	yfgM ← / ← hisS	ancillary SecYEG translocon subunit/histidine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,639,288	0	T	C	100.0%	156.5 / NA	45	intergenic (‑5/+13)	yfgM/hisS	ancillary SecYEG translocon subunit/histidine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (17/28);  total (17/28)

GTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGCCACTTCAGACTCACCCAGCA  >  NC_000913/2639155‑2639419                                                                                                                                      |                                                                                                                                    gTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGcccttct                                                                                                                               <  2:829857/140‑1 (MQ=255)  ttCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGcccttct                                                                                                                               <  2:716018/139‑1 (MQ=255)  ttCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGcccttct                                                                                                                               >  1:716018/1‑139 (MQ=255)  ttCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGccctactc                                                                                                                              >  1:229013/1‑140 (MQ=255)  ttCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACC‑GGTCGTTTTCGTTCTCGTAAATTTCCACGCTGcccttctcc                                                                                                                             <  2:83061/140‑1 (MQ=255)   tCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCCCCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGcccttctcc                                                                                                                             <  1:565323/140‑1 (MQ=255)   tCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGcccttctcc                                                                                                                             <  1:818/140‑1 (MQ=255)   tCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGcccttctcc                                                                                                                             <  1:241039/140‑1 (MQ=255)   tCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGcccttctcc                                                                                                                             <  1:6455/140‑1 (MQ=255)   tCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGcccttctcc                                                                                                                             <  1:323311/140‑1 (MQ=255)   tCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGcccttctcc                                                                                                                             <  1:465476/140‑1 (MQ=255)        tAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGCCCTTCtccttcct                                                                                                                        <  1:905213/140‑1 (MQ=255)        tAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGCCCTTCtccttcct                                                                                                                        <  2:435842/140‑1 (MQ=255)        tAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGCCCTTCtccttcct                                                                                                                        <  1:690888/140‑1 (MQ=255)        tAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGCCCTTCtccttcct                                                                                                                        <  1:30547/140‑1 (MQ=255)        tAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGCCCTACtccttcct                                                                                                                        <  2:229013/140‑1 (MQ=255)         aGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGCCCTTCTccttcctt                                                                                                                       <  2:970507/140‑1 (MQ=255)          gcgcCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGCCCTTCTCCTTCCTTa                                                                                                                      <  1:1087346/140‑1 (MQ=255)          gcgcCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGCCCTTCTCCTTCCTTa                                                                                                                      >  1:1229076/1‑140 (MQ=255)            gccagccaATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGcccttct                                                                                                                               <  2:148828/129‑1 (MQ=255)            gccagccaATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGcccttct                                                                                                                               >  1:148828/1‑129 (MQ=255)             ccagccaATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGCCCTTCtccttcct                                                                                                                        >  1:214578/1‑135 (MQ=255)             ccagccaATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGCCCTTCtccttcct                                                                                                                        <  2:214578/135‑1 (MQ=255)             ccagccaATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGCCCTTCTCCTTCCTTAAcc                                                                                                                   >  1:315570/1‑140 (MQ=255)             ccagccaATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGCCCTTCTCCTTCCTTAAcc                                                                                                                   >  1:1279262/1‑140 (MQ=255)                           cgcCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGCCCTTCTCCTTCCTTAACCCAGTAACGTGCGCa                                                                                                     >  1:812517/1‑140 (MQ=255)                           cgcCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGCCCTTCTCCTTCCTTAACCCAGTAACGTGCGCa                                                                                                     >  1:1260087/1‑140 (MQ=255)                               aaCGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGCCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATg                                                                                                 <  2:414673/140‑1 (MQ=255)                                 cGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGCCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATgcg                                                                                               <  1:1346033/140‑1 (MQ=255)                                 cGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTATCGT‑AATTTCCACGCTGCCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATgcgc                                                                                              <  1:28606/140‑1 (MQ=255)                                   cccAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGCCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGcgcg                                                                                             >  1:777050/1‑140 (MQ=255)                                    ccAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGCCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCgg                                                                                            <  1:318270/140‑1 (MQ=255)                                    ccAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGCCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCgg                                                                                            <  2:925163/140‑1 (MQ=255)                                    ccAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGCCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCgg                                                                                            <  2:315570/140‑1 (MQ=255)                                    ccAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGCCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCgg                                                                                            <  2:1287562/140‑1 (MQ=255)                                    ccAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGCCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCgg                                                                                            <  2:1279262/140‑1 (MQ=255)                                         aTCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGCCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACg                                                                                       <  2:843449/140‑1 (MQ=255)                                            aCCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGCCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTa                                                                                    >  2:840569/1‑140 (MQ=255)                                                          tGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGCCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGc                                                                      >  2:870068/1‑140 (MQ=255)                                                                gCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGCCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTg                                                                >  2:908995/1‑140 (MQ=255)                                                                        cAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGCCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCag                                                        <  1:819549/140‑1 (MQ=255)                                                                                   cGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGCCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATcc                                             <  2:812517/140‑1 (MQ=255)                                                                                   cGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGCCCTTCTCCTTCCTTAACCCAGTAACGCGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATcc                                             <  2:1260087/140‑1 (MQ=255)                                                                                     tttAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGCCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCtt                                           >  1:725171/1‑140 (MQ=255)                                                                                                       gTCGTTTTCGTTCTCGTAAATTTCCACGCTGCCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGtt                         >  1:1342082/1‑140 (MQ=255)                                                                                                       gTCGTTTTCGTTCTCGTAAATTTCCACGCTGCCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGtt                         <  1:870068/140‑1 (MQ=255)                                                                                                        tCGTTTTCGTTCTCGTAAATTTCCACGCTGCCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTa                        >  1:697097/1‑140 (MQ=255)                                                                                                        tCGTTTTCGTTCTCGTAAATTTCCACGCTGCCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTa                        >  2:1046451/1‑140 (MQ=255)                                                                                                          gTTTTCGTTCTCGTAAATTTCCACGCTGCCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGc                      <  1:908995/140‑1 (MQ=255)                                                                                                          gTTTTCGTTCTCGTAAATTTCCACGCTGCCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGc                      <  2:958958/140‑1 (MQ=255)                                                                                                             ttCGTTCTCGTAAATTTCCACGCTGCCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGCCAc                   >  2:53137/1‑140 (MQ=255)                                                                                                             ttCGTTCTCGTAAATTTCCACGCTGCCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGCCAc                   >  2:1101979/1‑140 (MQ=255)                                                                                                                gTTCTCGTAAATTTCCACGCTGCCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGCCACTTc                <  2:777050/140‑1 (MQ=255)                                                                                                                    tcGTAAATTTCCACGCTGCCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGCCACTTCAGAc            <  2:839676/140‑1 (MQ=255)                                                                                                                    tcGTAAATTTCCACGCTGCCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGCCACTTCAGAc            <  1:54189/140‑1 (MQ=255)                                                                                                                     cGTAAATTTCCACGCTGCCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGCCACTTCAGACt           <  2:427453/140‑1 (MQ=255)                                                                                                                     cGTAAATTTCCACGCTGCCCTTCTACTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGCCACTTCAGACt           >  2:1278935/1‑140 (MQ=255)                                                                                                                              ccACGCTGCCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGCCACTTCAGACTCACCCAGca  <  1:1294828/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                    GTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGCCACTTCAGACTCACCCAGCA  >  NC_000913/2639155‑2639419

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑