Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,718,354	G→A	P59S (CCC→TCC)	yfiF ←	putative methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,718,354	0	G	A	100.0%	178.7 / NA	51	P59S (CCC→TCC)	yfiF	putative methyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (26/25);  total (26/25)

GCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACA  >  NC_000913/2718219‑2718485                                                                                                                                        |                                                                                                                                    gCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGACTGa                                                                                                                                 <  2:536100/140‑1 (MQ=255) gCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGACTGa                                                                                                                                 <  2:490757/140‑1 (MQ=255)   tGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGgactgact                                                                                                                               >  2:1275253/1‑140 (MQ=255)                    cGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGgactgact                                                                                                                               >  1:828606/1‑123 (MQ=255)                    cGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGgactgact                                                                                                                               <  2:828606/123‑1 (MQ=255)                      gATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGACTGACTCTGTTTGTCATCgcgggcg                                                                                                            >  2:828603/1‑140 (MQ=255)                        tCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGACTGACTCTGt                                                                                                                           <  1:106488/123‑1 (MQ=255)                        tCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGACTGACTCTGt                                                                                                                           >  2:106488/1‑123 (MQ=255)                                    ttACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGACTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCAcc                                                                                              >  2:70265/1‑140 (MQ=255)                                     tACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGACTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCg                                                                                             >  1:315959/1‑140 (MQ=255)                                      aCCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGACTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGt                                                                                            >  1:113/1‑140 (MQ=255)                                          cTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGACTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGc                                                                                        >  1:28136/1‑140 (MQ=255)                                            gATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGACTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCAc                                                                                      >  2:313562/1‑140 (MQ=255)                                             aTGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGACTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACg                                                                                     >  2:47106/1‑140 (MQ=255)                                               gCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGACTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCa                                                                                                >  2:299959/1‑127 (MQ=255)                                               gCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGACTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCa                                                                                                <  1:299959/127‑1 (MQ=255)                                                      tGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGACTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTcc                                                                            >  2:149879/1‑140 (MQ=255)                                                        aTCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGACTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTg                                                                          >  2:1075450/1‑140 (MQ=255)                                                                tttCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGACTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGAcgcccg                                                                  >  2:480714/1‑140 (MQ=255)                                                                       cGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGACTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCtt                                                           <  1:974963/140‑1 (MQ=255)                                                                       cGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGACTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCtt                                                           >  2:725799/1‑140 (MQ=255)                                                                       cGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGACTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCtt                                                           >  2:736363/1‑140 (MQ=255)                                                                       cGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGACTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCtt                                                           >  1:1184857/1‑140 (MQ=255)                                                                         tctcATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGACTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTc                                                         <  1:480714/140‑1 (MQ=255)                                                                             atcaCCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGACTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCgg                                                     <  1:734752/140‑1 (MQ=255)                                                                                 cccGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGACTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTAcg                                                 <  1:1139532/140‑1 (MQ=255)                                                                                 cccGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGACTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTAcg                                                 <  1:116278/140‑1 (MQ=255)                                                                                  ccGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGACTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTAcgc                                                <  1:736363/140‑1 (MQ=255)                                                                                  ccGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGACTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTAcgc                                                <  1:1075450/140‑1 (MQ=255)                                                                                   cGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGACTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTAcgcg                                               <  1:1022460/140‑1 (MQ=255)                                                                                     gcgcgcGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGACTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCggg                                             >  1:274391/1‑140 (MQ=255)                                                                                      cgcgcgGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGACTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGt                                            <  1:1275253/140‑1 (MQ=255)                                                                                       gcgcgGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGACTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGtt                                           <  1:149879/140‑1 (MQ=255)                                                                                          cgGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGACTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTtgtg                                        >  2:522287/1‑140 (MQ=255)                                                                                          cgGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGACTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTACCTGGACG‑CCGCCCCCTTTCCCGGTAAGCGGGGTGTgg                                       >  2:613114/1‑140 (MQ=255)                                                                                           gggAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGACTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTgg                                       <  2:1324857/140‑1 (MQ=255)                                                                                                       gcCACGGCGAATCTTCCCACTTGCGGTCACGGGACTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCa                           <  2:1359004/140‑1 (MQ=255)                                                                                                       gcCACGGCGAATCTTCCCACTTGCGGTCACGGGACTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCa                           >  1:402344/1‑140 (MQ=255)                                                                                                       gcCACGGCGAATCTTCCCACTTGCGGTCACGGGACTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCa                           <  2:314429/140‑1 (MQ=255)                                                                                                       gcCACGGCGAATCTTCCCACTTGCGGTCACGGGACTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCa                           <  1:47106/140‑1 (MQ=255)                                                                                                       gcCACGGCGAATCTTCCCACTTGCGGTCACGGGACTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCa                           <  1:522287/140‑1 (MQ=255)                                                                                                         cACGGCGAATCTTCCCACTTGCGGTCACGGGACTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGa                         >  1:1026641/1‑140 (MQ=255)                                                                                                           cGGCGAATCTTCCCACTTGCGGTCACGGGACTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAat                       >  2:1320334/1‑140 (MQ=255)                                                                                                           cGGCGAATCTTCCCACTTGCGGTCACGGGACTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAat                       >  2:950621/1‑140 (MQ=255)                                                                                                              cGAATCTTCCCACTTGCGGTCACGGGACTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAatcat                    >  2:892627/1‑140 (MQ=255)                                                                                                                   cTTCCCACTTGCGGTCACGGGACTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAAtcatcatca               <  1:725799/140‑1 (MQ=255)                                                                                                                    ttCCCACTTGCGGTCACGGGACTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCAc              <  2:659027/140‑1 (MQ=255)                                                                                                                       ccACTTGCGGTCACGGGACTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGc           <  2:315959/140‑1 (MQ=255)                                                                                                                       ccACTTGCGGTCACGGGACTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGc           <  2:1026641/140‑1 (MQ=255)                                                                                                                         aCTTGCGGTCACGGGACTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCgg         <  2:113/140‑1 (MQ=255)                                                                                                                         aCTTGCGGTCACGGGACTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCgg         <  1:390755/140‑1 (MQ=255)                                                                                                                           ttGCGGTCACGGGACTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGac       >  1:1411536/1‑140 (MQ=255)                                                                                                                             gCGGTCACGGGACTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGacat     >  1:375891/1‑140 (MQ=255)                                                                                                                                gTCACGGGACTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGacataca  >  2:378697/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                    GCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACA  >  NC_000913/2718219‑2718485

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑