Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,930,807	T→C	L411P (CTG→CCG)	sdaB →	L‑serine deaminase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,930,807	0	T	C	100.0%	247.5 / NA	70	L411P (CTG→CCG)	sdaB	L‑serine deaminase II
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (32/38);  total (32/38)

GTGCATCGCGGCGGAAATCGCCATGGAGCACAACCTCGGTCTGACGTGTGACCCGGTCGCCGGACAGGTACAGGTGCCATGCATCGAGCGTAACGCCATTGCGGCAGTAAAAGCGGTGAACGCCGCACGTATGGCGCTGCGCCGTACCAGCGAGCCGCGCGTCTGCCTCGATAAAGTTATCGAAACCATGTACGAAACAGGTAAAGATATGAACGCCAAGTACCGCGAAACCTCTCGCGGCGGCCTGGCAATGAAGATCGTTGC  >  NC_000913/2930670‑2930933                                                                                                                                          |                                                                                                                               gtgCATCGCGGCGGAAATCGCCATGGAGCACAACCTCGGTCTGACGTGTGACCCGGTCGCCGGACAGGTACAGGTGCCATGCATCGAGCGTAACGCCATTGCGGCAGTAAAAGCGGTGAACGCCGCACGTATGGcgccgc                                                                                                                              >  2:540485/1‑140 (MQ=255)  tgCATCGCGGCGGAAATCGCCATGGAGCACAACCTCGGTCTGACGTGTGACCCGGTCGCCGGACAGGTACAGGTGCCATGCATCGAGCGTAACGCCATTGCGGCAGTAAAAGCGGTGAACGCCGCACGTATGGCGCcgcg                                                                                                                             <  1:1141241/140‑1 (MQ=255)  tgCATCGCGGCGGAAATCGCCATGGAGCACAACCTCGGTCTGACGTGTGACCCGGTCGCCGGACAGGTACAGGTGCCATGCATCGAGCGTAACGCCATTGCGGCAGTAAAAGCGGTGAACGCCGCACGTATGGCGCcgcg                                                                                                                             <  2:812008/140‑1 (MQ=255)    cATCGCGGCGGAAATCGCCATGGAGCACAACCTCGGTCTGACGTGTGACCCGGTCGCCGGACAGGTACAGGTGCCATGCATCGAGCGTAACGCCATTGCGGCAGTAAAAGCGGTGAACGCCGCACGTATGgcgccgcgcc                                                                                                                           >  2:782337/1‑140 (MQ=255)       cgcgGCGGAAATCGCCATGGAGCACAACCTCGGTCTGACGTGTGACCCGGTCGCCGGACAGGTACAGGTGCCATGCATCGAGCGTAACGCCATTGCGGCAGTAAAAGCGGTGAACGCCGCACGTATGGCGCCGCGCCGTa                                                                                                                        >  1:1261707/1‑140 (MQ=255)         cggcggAAATCGCCATGGAGCACAACCTCGGTCTGACGTGTGACCCGGTCGCCGGACAGGTACAGGTGCCATGCATCGAGCGTAACGCCATTGCGGCAGTAAAAGCGGTGAACGCCGCACGTATGGCGCCGCGCCGTAcc                                                                                                                      >  1:698703/1‑140 (MQ=255)         cggcggAAATCGCCATGGAGCACAACCTCGGTCTGACGTGTGACCCGGTCGCCGGACAGGTACAGGTGCCATGCATCGAGCGTAACGCCATTGCGGCAGTAAAAGCGGTGAACGCCGCACGTATGGCGCCGCGCCGTAcc                                                                                                                      >  2:1384255/1‑140 (MQ=255)         cggcggAAATCGCCATGGAGCACAACCTCGGTCTGACGTGTGACCCGGTCGCCGGACAGGTACAGGTGCCATGCATCGAGCGTAACGCCATTGCGGCAGTAAAAGCGGTGAACGCCGCACGTATGGCGCCGCGCCGTAcc                                                                                                                      >  2:82617/1‑140 (MQ=255)          ggcggAAATCGCCATGGAGCACAACCTCGGTCTGACGTGTGACCCGGTCGCCGGACAGGTACAGGTGCCATGCATCGAGCGTAACGCCATTGCGGCAGTAAAAGCGGTGAACGCCGCACGTATGGCGCCGCGCCGTACCa                                                                                                                     >  1:665048/1‑140 (MQ=255)             ggAAATCGCCATGGAGCACAACCTCAGTCTGACGTGTGACCCGGTCGCCGGACAGGTACAGGTGCCATGCATCGAGCGTAACGCCATTGCGGCAGTAAAAGCGGTGAACGCCGCACGTATGGCGCCGCGCCGTACCAGCg                                                                                                                  <  1:292427/140‑1 (MQ=255)                  tCGCCATGGAGCACAACCTCGGTCTGACGTGTGACCCGGTCGCCGGACAGGTACAGGTGCCATGCATCGAGCGTAACGCCATTGCGGCAGTAAAAGCGGTGAACGCCGCACGTATGGCGCCGCGCCGTACCAGCGAGCcg                                                                                                             <  1:76281/140‑1 (MQ=255)                     ccATGGAGCACAACCTCGGTCTGACGTGTGACCCGGTCGCCGGACAGGTACAGGTGCCATGCATCGAGCGTAACGCCATTGCGGCAGTAAAAGCGGTGAACGCCGCACGTATGGCGCCGCGCCGTACCAGCGAGCcgcgc                                                                                                          <  2:1261707/140‑1 (MQ=255)                          gAGCACAACCTCGGTCTGACGTGTGACCCGGTCGCCGGACAGGTACAGGTGCCATGCATCGAGCGTAACGCCATTGCGGCAGTAAAAGCGGTGAACGCCGCACGTATGGCGCCGCGCCGTACCAGCGAGCCGCGCGTCTg                                                                                                     <  2:264677/140‑1 (MQ=255)                          gAGCACAACCTCGGTCTGACGTGTGACCCGGTCGCCGGACAGGTACAGGTGCCATGCATCGAGCGTAACGCCATTGCGGCAGTAAAAGCGGTGAACGCCGCACGTATGGCGCCGCGCCGTACCAGCGAGCCGCGCGTCTg                                                                                                     <  2:1264298/140‑1 (MQ=255)                           aGCACAACCTCGGTCTGACGTGTGACCCGGTCGCCGGACAGGTACAGGTGCCATGCATCGAGCGTAACGCCATTGCGGCAGTAAAAGCGGTGAACGCCGCACGTATGGCGCCGCGCCGTACCAGCGAGCCGCGCGTCTGc                                                                                                    <  1:116367/140‑1 (MQ=255)                             cacaACCTCGGTCTGACGTGTGACCCGGTCGCCGGACAGGTACAGGTGCCATGCATCGAGCGTAACGCCATTGCGGCAGTAAAAGCGGTGAACGCCGCACGTATGGCGCCGCGCCGTACCAGCGAGCCGCGCGTCTGCCt                                                                                                  >  2:830961/1‑140 (MQ=255)                                aaCCTCGGTCTGACGTGTGACCCGGTCGCCGGACAGGTACAGGTGCCATGCATCGAGCGTAACGCCATTGCGGCAGTAAAAGCGGTGAACGCCGCACGTATGGCGCCGCGCCGTACCAGCGAGCCGCGCGTCTGCCTCGa                                                                                               >  1:815979/1‑140 (MQ=255)                                  ccTCGGTCTGACGTGTGACCCGGTCGCCGGACAGGTACAGGTGCCATGCATCGAGCGTAACGCCATTGCGGCAGTAAAAGCGGTGAACGCCGCACGTATGGCGCCGCGCCGTACCAGCGAGCCGCGCGTCTGCCTCGATa                                                                                             >  2:149511/1‑140 (MQ=255)                                  ccTCGGTCTGACGTGTGACCCGGTCGCCGGACAGGTACAGGTGCCATGCATCGAGCGTAACGCCATTGCGGCAGTAAAAGCGGTGAACGCCGCACGTATGGCGCCGCGCCGTACCAGCGAGCCGCGCGTCTGCCTCGATa                                                                                             <  2:492588/140‑1 (MQ=255)                                    tCGGTCTGACGTGTGACCCGGTCGCCGGACAGGTACAGGTGCCATGCATCGAGCGTAACGCCATTGCGGCAGTAAAAGCGGTGAACGCCGCACGTATGGCGCCGCGCCGTACCAGCGAGCCGCGCGTCTGCCTCGATaaa                                                                                           <  2:314630/140‑1 (MQ=255)                                    tCGGTCTGACGTGTGACCCGGTCGCCGGACAGGTACAGGTGCCATGCATCGAGCGTAACGCCATTGCGGCAGTAAAAGCGGTGAACGCCGCACGTATGGCGCCGCGCCGTACCAGCGAGCCGCGCGTCTGCCTCGATaaa                                                                                           <  2:157191/140‑1 (MQ=255)                                       gTCTGACGTGTGACCCGGTCGCCGGACAGGTACAGGTGCCATGCATCGAGCGTAACGCCATTGCGGCAGTAAAAGCGGTGAACGCCGCACGTATGGCGCCGCGCCGTACCAGCGAGCCGCGCGTCTGCCTCGATAAAGtt                                                                                        >  1:633692/1‑140 (MQ=255)                                           gACGTGTGACCCGGTCGCCGGACAGGTACAGGTGCCATGCATCGAGCGTAACGCCATTGCGGCAGTAAAAGCGGTGAACGCCGCACGTATGGCGCCGCGCCGTACCAGCGAGCCGCGCGTCTGCCTCGATAAAGTTATCg                                                                                    <  2:1340106/140‑1 (MQ=255)                                           gACGTGTGACCCGGTCGCCGGACAGGTACAGGTGCCATGCATCGAGCGTAACGCCATTGCGGCAGTAAAAGCGGTGAACGCCGCACGTATGGCGCCGCGCCGTACCAGCGAGCCGCGCGTCTGCCTCGATAAAGTTATCg                                                                                    <  1:155140/140‑1 (MQ=255)                                            aCGTGTGACCCGGTCGCCGGACAGGTACAGGTGCCATGCATCGAGCGTAACGCCATTGCGGCAGTAAAAGCGGTGAACGCCGCACGTATGGCGCCGCGCCGTACCAGCGAGCCGCGCGTCTGCCTCGATAAAGTTATCGa                                                                                   >  2:739601/1‑140 (MQ=255)                                              gtgtGACCCGGTCGCCGGACAGGTACAGGTGCCATGCATCGAGCGTAACGCCATTGCGGCAGTAAAAGCGGTGAACGCCGCACGTATGGCGCCGCGCCGTACCAGCGAGCCGCGCGTCTGCCTCGATAAAGTTATCGaaa                                                                                 <  2:1059943/140‑1 (MQ=255)                                                gtgACCCGGTCGCCGGACAGGTACAGGTGCCATGCATCGAGCGTAACGCCATTGCGGCAGTAAAAGCGGTGAACGCCGCACGTATGGCGCCGCGCCGTACCAGCGAGCCGCGCGTCTGCCTCGATAAAGTTATCGAAAcc                                                                               <  1:1116624/140‑1 (MQ=255)                                                gtgACCCGGTCGCCGGACAGGTACAGGTGCCATGCATCGAGCGTAACGCCATTGCGGCAGTAAAAGCGGTGAACGCCGCACGTATGGCGCCGCGCCGTACCAGCGAGCCGCGCGTCTGCCTCGATAAAGTTATCGAAAcc                                                                               <  2:665048/140‑1 (MQ=255)                                                gtgACCCGGTCGCCGGACAGGTACAGGTGCCATGCATCGAGCGTAACGCCATTGCGGCAGTAAAAGCGGTGAACGCCGCACGTATGGCGCCGCGCCGTACCAGCGAGCCGCGCGTCTGCCTCGATAAAGTTATCGAAAcc                                                                               <  1:830961/140‑1 (MQ=255)                                                 tgACCCGGTCGCCGGACAGGTACAGGTGCCATGCATCGAGCGTAACGCCATTGCGGCAGTAAAAGCGGTGAACGCCGCACGTATGGCGCCGCGCCGTACCAGCGAGCCGCGCGTCTGCCTCGATAAAGTTATCGAAACCa                                                                              >  1:1129086/1‑140 (MQ=255)                                                  gACCCGGTCGCCGGACAGGTACAGGTGCCATGCATCGAGCGTAACGCCATTGCGGCAGTAAAAGCGGTGAACGCCGCACGTATGGCGCCGCGCCGTACCAGCGAGCCGCGCGTCTGCCTCGATAAAGTTATCGAAACCAt                                                                             >  1:1112688/1‑140 (MQ=255)                                                      cGGTCGCCGGACAGGTACAGGTGCCATGCATCGAGCGTAACGCCATTGCGGCAGTAAAAGCGGTGAACGCCGCACGTATGGCGCCGCGCCGTACCAGCGAGCCGCGCGTCTGCCTTGATAAAGTTATCg                                                                                    >  2:252438/1‑129 (MQ=255)                                                      cGGTCGCCGGACAGGTACAGGTGCCATGCATCGAGCGTAACGCCATTGCGGCAGTAAAAGCGGTGAACGCCGCACGTATGGCGCCGCGCCGTACCAGCGAGCCGCGCGTCTGCCTTGATAAAGTTATCg                                                                                    <  1:252438/129‑1 (MQ=255)                                                         tCGCCGGACAGGTACAGGTGCCATGCATCGAGCGTAACGCCATTGCGGCAGTAAAAGCGGTGAACGCCGCACGTATGGCGCCGCGCCGTACCAGCGAGCCGCGCGTCTGCCTCGATAAAGTTATCGAAACCATGTACGag                                                                      >  2:500816/1‑139 (MQ=255)                                                         tCGCCGGACAGGTACAGGTGCCATGCATCGAGCGTAACGCCATTGCGGCAGTAAAAGCGGTGAACGCCGCACGTATGGCGCCGCGCCGTACCAGCGAGCCGCGCGTCTGCCTCGATAAAGTTATCGAAACCATGTACGaa                                                                      <  1:93449/140‑1 (MQ=255)                                                          cGCCGGACAGGTACAGGTGCCATGCATCGAGCGTAACGCCATTGCGGCAGTAAAAGCGGTGAACGCCGCACGTATGGCGCCGCGCCGTACCAGCGAGCCGCGCGTCTGCCTCGATAAAGTTATCGAAACCATGTACGaaa                                                                     <  2:233540/140‑1 (MQ=255)                                                          cGCCGGACAGGTACAGGTGCCATGCATCGAGCGTAACGCCATTGCGGCAGTAAAAGCGGTGAACGCCGCACGTATGGCGCCGCGCCGTACCAGCGAGCCGCGCGTCTGCCTCGATAAAGTTATCGAAACCATGTACGaaa                                                                     <  1:782337/140‑1 (MQ=255)                                                           gCCGGACAGGTACAGGTGCCATGCATCGAGCGTAACGCCATTGCGGCAGTAAAAGCGGTGAACGCCGCACGTATGGCGCCGCGCCGTACCAGCGAGCCGCGCGTCTGCCTCGATAAAGTTATCGAAACCATGTACGAAAc                                                                    <  2:633692/140‑1 (MQ=255)                                                           gCCGGACAGGTACAGGTGCCATGCATCGAGCGTAACGCCATTGCGGCAGTAAAAGCGGTGAACGCCGCACGTATGGCGCCGCGCCGTACCAGCGAGCCGCGCGTCTGCCTCGATAAAGTTATCGAAACCATGTACGAAAc                                                                    <  1:267428/140‑1 (MQ=255)                                                               gACAGGTACAGGTGCCATGCATCGAGCGTAACGCCATTGCGGCAGTAAAAGCGGTGAACGCCGCACGTATGGCGCCGCGCCGTACCAGCGAGCCGCGCGTCTGCCTCGATAAAGTTATCGAAACCATGTACGAGACAGGt                                                                <  1:500816/140‑1 (MQ=255)                                                               gACAGGTACAGGTGCCATGCATCGAGCGTAACGCCATTGCGGCAGTAAAAGCGGTGAACGCCGCACGTATGGCGCCGCGCCGTACCAGCGAGCCGCGCGTCTGCCTCGATAAAGTTATCGAAACCATGTACGAAACAGGt                                                                <  1:540485/140‑1 (MQ=255)                                                               gACAGGTACAGGTGCCATGCATCGAGCGTAACGCCATTGCGGCAGTAAAAGCGGTGAACGCCGCACGTATGGCGCCGCGCCGTACCAGCGAGCCGCGCGTCTGCCTCGATAAAGTTATCGAAACCATGTACGAAACAGGt                                                                <  2:122946/140‑1 (MQ=255)                                                               gACAGGTACAGGTGCCATGCATCGAGCGTAACGCCATTGCGGCAGTAAAAGCGGTGAACGCCGCACGTATGGCGCCGCGCCGTACCAGCGAGCCGCGCGTCTGCCTCGATAAAGTTATCGAAACCATGTACGAAACAGGt                                                                <  1:1018853/140‑1 (MQ=255)                                                                acaggtacaggtGCCATGCATCGAGCGTAACGCCATTGCGGCAGTAAAAGCGGTGAACGCCGCACGTATGGCGCCGCGCCGTACCAGCGAGCCGCGCGTCTGCCTCGATAAAGTTATCGAAACCATGTACGAAACAGGTa                                                               <  1:409487/140‑1 (MQ=255)                                                                   ggtacaggtGCCATGCATCGAGCGTAACGCCATTGCGGCAGTAAAAGCGGTGAACGCCGCACGTATGGCGCCGCGCCGTACCAGCGAGCCGCGCGTCTGCCTCGATAAAGTTATCGAAACCATGTACGAAACAGGTAAAg                                                            >  2:1388861/1‑140 (MQ=255)                                                                       caggtGCCATGCATCGAGCGTAACGCCATTGCGGCAGTAAAAGCGGTGAACGCCGCACGTATGGCGCCGCGCCGTACCAGCGAGCCGCGCGTCTGCCTCGATAAAGTTATCGAAACCATGTACGAAACAGGTAAAGatat                                                        <  2:519369/140‑1 (MQ=255)                                                                         ggtGCCATGCATCGAGCGTAACGCCATTGCGGCAGTAAAAGCGGTGAACGCCGCACGTATGGCGCCGCGCCGTACCAGCGAGCCGCGCGTCTGCCTCGATAAAGTTATCGAAACCATGTACGAAACAGGTAAAGATATGa                                                      <  1:387884/140‑1 (MQ=255)                                                                          gtGCCATGCATCGAGCGTAACGCCATTGCGGCAGTAAAAGCGGTGAACGCCGCACGTATGGCGCCGCGCCGTACCAGCGAGCCGCGCGTCTGCCTCGATAAAGTTATCGAAACCATGTACGAAACAGGTAAAGATATGaa                                                     >  2:92260/1‑140 (MQ=255)                                                                            gCCATGCATCGAGCGTAACGCCATTGCGGCAGTAAAAGCGGTGAACGCCGCACGTATGGCGCCGCGCCGTACCAGCGAGCCGCGCGTCTGCCTCGATAAAGTTATCGAAACCATGTACGAAACAGGTAAAGATATGAACg                                                   >  1:688419/1‑140 (MQ=255)                                                                            gCCATGCATCGAGCGTAACGCCATTGCGGCAGTAAAAGCGGTGAACGCCGCACGTATGGCGCCGCGCCGTACCAGCGAGCCGCGCGTCTGCCTCGATAAAGTTATCGAAACCATGTACGAAACAGGTAAAGATATGAACg                                                   >  2:935807/1‑140 (MQ=255)                                                                              cATGCATCGAGCGTAACGCCATTGCGGCAGTAAAAGCGGTGAACGCCGCACGTATGGCGCCGCGCCGTACCAGCGAGCCGCGCGTCTGCCTCGATAAAGTTATCGAAACCATGTACGAAACAGGTAAAGATATGAACGcc                                                 >  2:74226/1‑140 (MQ=255)                                                                              cATGCATCGAGCGTAACGCCATTGCGGCAGTAAAAGCGGTGAACGCCGCACGTATGGCGCCGCGCCGTACCAGCGAGCCGCGCGTCTGCCTCGATAAAGTTATCGAAACCATGTACGAAACAGGTAAAGATATGAACGcc                                                 >  2:401282/1‑140 (MQ=255)                                                                              cATGCATCGAGCGTAACGCCATTGCGGCAGTAAAAGCGGTGAACGCCGCACGTATGGCGCCGCACCGTACCAGCGAGCCGCGCGTCTGCCTCGATAAAGTTATCGAAACCATGTACGAAACAGGTAAAGATATGAACGcc                                                 >  1:1021004/1‑140 (MQ=255)                                                                               aTGCATCGAGCGTAACGCCATTGCGGCAGTAAAAGCGGTGAACGCCGCACGTATGGCGCCGCGCCGTACCAGCGAGCCGCGCGTCTGCCTCGATAAAGTTATCGAAACCATGTACGAAACAGGTAAAGATATGAACGCCa                                                >  2:464217/1‑140 (MQ=255)                                                                               aTGCATCGAGCGTAACGCCATTGCGGCAGTAAAAGCGGTGAACGCCGCACGTATGGCGCCGCGCCGTACCAGCGAGCCGCGCGTCTGCCTCGATAAAGTTATCGAAACCATGTACGAAACAGGTAAAGATATGAACGCCa                                                >  1:620443/1‑140 (MQ=255)                                                                                tGCATCGAGCGTAACGCCATTGCGGCAGTAAAAGCGGTGAACGCCGCACGTATGGCGCCGCGCCGTACCAGCGAGCCGCGCGTCTGCCTCGATAAAGTTATCGAAACCATGTACGAAACAGGTAAAGATATGAACGCCaa                                               <  2:398144/140‑1 (MQ=255)                                                                                  cATCGAGCGTAACGCCATTGCGGCAGTAAAAGCGGTGAACGCCGCACGTATGGCGCCGCGCCGTACCAGCGAGCCGCGCGTCTGCCTCGATAAAGTTATCGAAACCATGTACGAAACAGGTAAAGATATGAACGCCAAGt                                             <  2:506316/140‑1 (MQ=255)                                                                                   aTCGAGCGTAACGCCATTGCGGCAGTAAAAGCGGTGAACGCCGCACGTATGGCGCCGCGCCGTACCAGCGAGCCGCGCGTCTGCCTCGATAAAGTTATCGAAACCATGTACGAAACAGGTAAAGATATGAACGCCAAGTa                                            >  2:284832/1‑140 (MQ=255)                                                                                    tCGAGCGTAACGCCATTGCGGCAGTAAAAGCGGTGAACGCCGCACGTATGGCGCCGTGCCGTACCAGCGAGCCGCGCGTCTGCCTCGATAAAGTTATCGAAACCATGTACGAAACAGGTAAAGATATGAACGCCAAGTAc                                           >  1:1395205/1‑140 (MQ=255)                                                                                            aaCGCCATTGCGGCAGTAAAAGCGGTGAACGCCGCACGTATGGCGCCGCGCCGTACCAGCGAGCCGCGCGTCTGCCTCGATAAAGTTATCGAAACCATGTACGAAACAGGTAAAGATATGAACGCCAAGTACCGCGAAAc                                   >  2:35122/1‑140 (MQ=255)                                                                                               gCCATTGCGGCAGTAAAAGCGGTGAACGCCGCACGTATGGCGCCGCGCCGTACCAGCGAGCCGCGCGTCTGCCTTGATAAAGTTATCGAAACCATGTACGAAACAGGTAAAGATATGAACGCCAAGTACCGCGAAACctc                                <  2:1241906/140‑1 (MQ=255)                                                                                                      cGGCAGTAAAAGCGGTGAACGCCGCACGTATGGCGCCGCGCCGTACCAGCGAGCCGCGCGTCTGCCTCGATAAAGTTATCGAAACCATGTACGAAACAGGTAAAGATATGAACGCCAAGTACCGCGAAACCTCTCgcggc                         >  1:728394/1‑140 (MQ=255)                                                                                                      cGGCAGTAAAAGCGGTGAACGCCGCACGTATGGCGCCGCGCCGTACCAGCGAGCCGCGCGTCTGCCTCGATAAAGTTATCGAAACCATGTACGAAACAGGTAAAGATATGAACGCCAAGTACCGCGAAACCTCTCgcggc                         >  2:882853/1‑140 (MQ=255)                                                                                                      cGGCAGTAAAAGCGGTGAACGCCGCACGTATGGCGCCGCGCCGTACCAGCGAGCCGCGCGTCTGCCTCGATAAAGTTATCGAAACCATGTACGAAACAGGTAAAGATATGAACGCCAAGTACCGCGAAACCTCTCgcggc                         >  2:121947/1‑140 (MQ=255)                                                                                                            tagaaGCGGTGAACGCCGCACGTATGGCGCCGCGCCGTACCAGCGAGCCGCGCGTCTGCCTCGATAAAGTTATCGAAACCATGTACGAAACAGGTAAAGATATGAACGCCAAGTACCGCGAAACCTCTCGCGGCGGCCTg                   >  1:51332/4‑140 (MQ=255)                                                                                                            tAAAAGCGGTGAACGCCGCACGTATGGCGCCGCGCCGTACCAGCGAGCCGCGCGTCTGCCTCGATAAAGTTATCGAAACCATGTACGAAACAGGTAAAGATATGAACGCCAAGTACCGCGAAACCTCTCGCGGCGGCCTg                   <  1:401282/140‑1 (MQ=255)                                                                                                             aaaaGCGGTGAACGCCGCACGTATGGCGCCGCGCCGTACCAGCGAGCCGCGCGTCTGCCTCGATAAAGTTATCGAAACCATGTACGAAACAGGTAAAGATATGAACGCCAAGTACCGCGAAACCTCTCGCGGCGGCCTgg                  <  2:1112688/140‑1 (MQ=255)                                                                                                             aaaaGCGGTGAACGCCGCACGTATGGCGCCGCGCCGTACCAGCGAGCCGCGCGTCTGCCTCGATAAAGTTATCGAAACCATGTACGAAACAGGTAAAGATATGAACGCCAAGTACCGCGAAACCTCTCGCGGCGGCCTgg                  <  2:796642/140‑1 (MQ=255)                                                                                                             aaaaGCGGTGAACGCCGCACGTATGGCGCCGCACCGTACCAGCGAGCCGCGCGTCTGCCTCGATAAAGTTATCGAAACCATGTACGAAACAGGTAAAGATATGAACGCCAAGTACCGCGAAACCTCTCGCGGCGGCCTgg                  <  2:1021004/140‑1 (MQ=255)                                                                                                                 gCGGTGAACGCCGCACGTATGGCGCCGCGCCGTACCAGCGAGCCGCGCGTCTGCCTCGATAAAGTTATCGAAACCATGTACGAAACAGGTAAAGATATGAACGCCAAGTACCGCGAAACCTCTCGCGGCGGCCTGGCAAt              <  2:470796/140‑1 (MQ=255)                                                                                                                   ggTGAACGCCGCACGTATGGCGCCGCGCCGTACCAGCGAGCCGCGCGTCTGCCTCGATAAAGTTATCGAAACCATGTACGAAACAGGTAAAGATATGAACGCCAAGTACCGCGAAACCTCTCGCGGCGGCCTGGCAATGa            >  1:1214251/1‑140 (MQ=255)                                                                                                                      gAACGCCGCACGTATGGCGCCGCGCCGTACCAGCGAGCCGCGCGTCTGCCTCGATAAAGTTATCGAAACCATGTACGAAACAGGTAAAGATATGAACGCCAAGTACCGCGAAACCTCTCGCGGCGGCCTGGCAATGAAGa         >  1:1033984/1‑140 (MQ=255)                                                                                                                             gcACGTATGGCGCCGCGCCGTACCAGCGAGCCGCGCGTCTGCCTCGATAAAGTTATCGAAACCATGTACGAAACAGGTAAAGATATGAACGCCAAGTACCGCGAAACCTCTCGCGGCGGCCTGGCAATGAAGATCGTTGc  <  1:149511/140‑1 (MQ=255)                                                                                                                             gcACGTATGGCGCCGCGCCGTACCAGCGAGCCGCGCGTCTGCCTCGATAAAGTTATCGAAACCATGTACGAAACAGGTAAAGATATGAACGCCAAGTACCGCGAAACCTCTCGCGGCGGCCTGGCAATGAAGATCGTTGc  <  1:1117174/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                               GTGCATCGCGGCGGAAATCGCCATGGAGCACAACCTCGGTCTGACGTGTGACCCGGTCGCCGGACAGGTACAGGTGCCATGCATCGAGCGTAACGCCATTGCGGCAGTAAAAGCGGTGAACGCCGCACGTATGGCGCTGCGCCGTACCAGCGAGCCGCGCGTCTGCCTCGATAAAGTTATCGAAACCATGTACGAAACAGGTAAAGATATGAACGCCAAGTACCGCGAAACCTCTCGCGGCGGCCTGGCAATGAAGATCGTTGC  >  NC_000913/2930670‑2930933

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑