Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,008,054	A→G	H182R (CAC→CGC)	ygeX →	2,3‑diaminopropionate ammonia‑lyase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,008,054	0	A	G	100.0%	256.9 / NA	73	H182R (CAC→CGC)	ygeX	2,3‑diaminopropionate ammonia‑lyase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (43/30);  total (43/30)

TGCGGTGATTTACATGCCGAAAGGTTCTGCTCAGGAACGCGTTGACGCCATTCTGAACCTCGGTGCCGAGTGCATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGACACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGCAGATGAAGCCGTCGAGCAAATGCGTGAAATGGGCGTAACCCCGACGCACG  >  NC_000913/3007920‑3008188                                                                                                                                       |                                                                                                                                       tGCGGTGATTTACATGCCGAAAGGTTCTGCTCAGGAACGCGTTGACGCCATTCTGAACCTCGGTGCCGAGTGCATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCGCGGCt                                                                                                                                   >  2:911349/1‑140 (MQ=255)       gATTTACATGCCGAAAGGTTCTGCTCAGGAACGCGTTGACGCCATTCTGAACCTCGGTGCCGAGTGCATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCGCGGCTGGGAAg                                                                                                                             <  1:598897/140‑1 (MQ=255)       gATTTACATGCCGAAAGGTTCTGCTCAGGAACGCGTTGACGCCATTCTGAACCTCGGTGCCGAGTGCATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCGCGGCTGGGAAg                                                                                                                             >  2:275171/1‑140 (MQ=255)       gATTTACATGCCGAAAGGTTCTGCTCAGGAACGCGTTGACGCCATTCTGAACCTCGGTGCCGAGTGCATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCGCGGCTGGGAAg                                                                                                                             <  1:601214/140‑1 (MQ=255)       gATTTACATGCCGAAAGGTTCTGCTCAGGAACGCGTTGACGCCATTCTGAACCTCGGTGCCGAGTGCATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCGCGGCTGGGAAg                                                                                                                             <  2:328931/140‑1 (MQ=255)            aCATGCCGAAAGGTTCTGCTCAGGAACGCGTTGACGCCATTCTGAACCTCGGTGCCGAGTGCATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCGCGGCTGGGAAGTGGTa                                                                                                                        <  2:1166157/140‑1 (MQ=255)              aTGCCGAAAGGTTCTGCTCAGGAACGCGTTGACGCCATTCTGAACCTCGGTGCCGAGTGCATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCGCGGCTGGGAAGTGGTACa                                                                                                                      <  2:1021486/140‑1 (MQ=255)                gCCGAAAGGTTCTGCTCAGGAACGCGTTGACGCCATTCTGAACCTCGGTGCCGAGTGCATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCGCGGCTGGGAAGTGGTa                                                                                                                        >  1:100423/1‑136 (MQ=255)                gCCGAAAGGTTCTGCTCAGGAACGCGTTGACGCCATTCTGAACCTCGGTGCCGAGTGCATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCGCGGCTGGGAAGTGGTa                                                                                                                        <  2:100423/136‑1 (MQ=255)                 ccGAAAGGTTCTGCTCAGGAACGCGTTGACGCCATTCTGAACCTCGGTGCCGAGTGCATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCGCGGCTGGGAAGTGGTACAGGa                                                                                                                   <  2:901393/140‑1 (MQ=255)                  cGAAAGGTTCTGCTCAGGAACGCGTTGACGCCATTCTGAACCTCGGTGCCGAGTGCATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCGCGGCTGGGAAGTGGTACAGGac                                                                                                                  >  2:293317/1‑140 (MQ=255)                  cGAAAGGTTCTGCTCAGGAACGCGTTGACGCCATTCTGAACCTCGGTGCCGAGTGCATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCGCGGCTGGGAAGTGGTACAGGac                                                                                                                  >  1:1357375/1‑140 (MQ=255)                   gAAAGGTTCTGCTCAGGAACGCGTTGACGCCATTCTGAACCTCGGTGCCGAGTGCATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCGCGGCTGGGAAGTGGTACAGGaca                                                                                                                 <  1:165640/140‑1 (MQ=255)                    aaaGGTTCTGCTCAGGAACGCGTTGACGCCATTCTGAACCTCGGTGCCGAGTGCATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCGCGGCTGGGAAGTGGTACAGGacac                                                                                                                >  1:236557/1‑140 (MQ=255)                    aaaGGTTCTGCTCAGGAACGCGTTGACGCCATTCTGAACCTCGGTGCCGAGTGCATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCGCGGCTGGGAAGTGGTACAGGacac                                                                                                                >  1:159804/1‑140 (MQ=255)                           cTGCTCAGGAACGCGTTGACGCTATTCTGAACCTCGGTGCCGAGTGCATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCGCGGCTGGGAAGTGGTACAGGACACGGCATgg                                                                                                         <  1:997063/140‑1 (MQ=255)                                   gaccgcgTTGACGCCATTCTGAACCTCGGTGCCGAGTGCATCGTCACGGTTATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCGCGGCTGGGAAGTGGTACAGGACACGGCATGGGAAGGTTa                                                                                                 >  2:235713/4‑140 (MQ=255)                                   gAACGCGTTGACGCCATTCTGAACCTCGGTGCCGAGTGCATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCGCGGCTGGGAAGTGGTACAGGACACGGCATGGGAAGGTTa                                                                                                 >  1:334820/1‑140 (MQ=255)                                   gAACGCGTTGACGCCATTCTGAACCTCGGTGCCGAGTGCATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCGCGGCTGGGAAGTGGTACAGGACACGGCATGGGAAGGTTa                                                                                                 >  2:803911/1‑140 (MQ=255)                                    aaCGCGTTGACGCCATTCTGAACCTCGGTGCCGAGTGCATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCGCGGCTGGGAAGTGGTACAGGACACGGCATGGGAAGGTTac                                                                                                <  2:447013/140‑1 (MQ=255)                                      cgcgTTGACGCCATTCTGAACCTCGGTGCCGAGTGCATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCGCGGCTGGGAAGTGGTACAGGACACGGCATGGGAAGGTTacac                                                                                              >  2:577890/1‑140 (MQ=255)                                        cgTTGACGCCATTCTGAACCTCGGTGCCGAGTGCATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCGCGGCTGGGAAGTGGTACAGGACACGGCATGGGAAGGt                                                                                                   <  2:1411989/133‑1 (MQ=255)                                        cgTTGACGCCATTCTGAACCTCGGTGCCGAGTGCATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCGCGGCTGGGAAGTGGTACAGGACACGGCATGGGAAGGt                                                                                                   >  1:1411989/1‑133 (MQ=255)                                        cgTTGACGCCATTCTGAACCTCGGTGCCGAGTGCATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCGCGGCTGGGAAGTGGTACAGGACACGGCATGGGAAGGTTACACCa                                                                                            >  2:1030028/1‑140 (MQ=255)                                             aCGCCATTCTGAACCTCGGTGCCGAGTGCATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCGCGGCTGGGAAGTGGTACAGGACACGGCATGGGAAGGTTACACCAAAATc                                                                                       <  2:957781/140‑1 (MQ=255)                                             aCGCCATTCTGAACCTCGGTGCCGAGTGCATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATACAACACGCGCAGCAGCGCGGCTGGGAAGTGGTACAGGACACGGCATGGGAAGGTTACACCAAAATc                                                                                       <  1:221753/140‑1 (MQ=255)                                              cGCCATTCTGAACCTCGGTGCCGAGTGCATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCGCGGCTGGGAAGTGGTACAGGACACGGCATGGGAAGGTTACACCAAAATcc                                                                                      <  2:465136/140‑1 (MQ=255)                                                 cATTCTGAACCTCGGTGCCGAGTGCATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCGCGGCTGGGAAGTGGTACAGGACACGGCATGGGAAGGTTACACCAAAATCCCaa                                                                                   >  2:303664/1‑140 (MQ=255)                                                 cATTCTGAACCTCGGTGCCGAGTGCATCGTCACGGATATGAACTATGACGATACCGTCCGCCTGACCATGCAACACGCGCAGCAGCGCGGCTGGGAAGTGGTACAGGACACGGCATGGGAAGGTTACACCAAAATCCCaa                                                                                   >  2:15506/1‑140 (MQ=255)                                                  aTTCTGAACCTCGGTGCCGAGTGCATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCGCGGCTGGGAAGTGGTACAGGACACGGCATGGGAAGGTTACACCAAAATCCCAAc                                                                                  <  2:159804/140‑1 (MQ=255)                                                  aTTCTGAACCTCGGTGCCGAGTGCATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCGCGGCTGGGAAGTGGTACAGGACACGGCATGGGAAGGTTACACCAAAATCCCAAc                                                                                  <  1:956058/140‑1 (MQ=255)                                                  aTTCTGAACCTCGGTGCCGAGTGCATCGTCACGGATATGAACTATGACGATACCGTCCGCCTGACCATGCAACACGCGCAGCAGCGCGGCTGGGAAGTGGTACAGGACACGGCATGGGAAGGTTACACCAAAATCCCAAc                                                                                  <  1:15506/140‑1 (MQ=255)                                                       gAACCTCGGTGCCGAGTGCATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCGCGGCTGGGAAGTGGTACAGGACACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGa                                                                             <  1:911349/140‑1 (MQ=255)                                                             cGGTGCCGAGTGCATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCGCGGCTGGGAAGTGGTACAGGACACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGc                                                                       >  1:21089/1‑140 (MQ=255)                                                             cGGTGCCGAGTGCATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCGCGGCTGGGAAGTGGTACAGGACACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGc                                                                       >  1:864869/1‑140 (MQ=255)                                                             cGGTGCCGAGTGCATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCGCGGCTGGGAAGTGGTACAGGACACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGc                                                                       >  1:820382/1‑140 (MQ=255)                                                                 gCCGAGTGCATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCGCGGCTGGGAAGTGGTACAGGACACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAgg                                                                   >  1:1268811/1‑140 (MQ=255)                                                                 gCCGAGTGCATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCGCGGCTGGGAAGTGGTACAGGACACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAgg                                                                   <  2:820382/140‑1 (MQ=255)                                                                  ccGAGTGCATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCGCGGCTGGGAAGTGGTACAGGACACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGc                                                                  >  2:872860/1‑140 (MQ=255)                                                                     aGTGCATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCGCGGCTGGGAAGTGGTACAGGACACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTAc                                                               <  2:334820/140‑1 (MQ=255)                                                                          aTCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCGCGGCTGGGAAGTGGTACAGGACACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAAc                                                          >  2:305090/1‑140 (MQ=255)                                                                           tCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCGCGGCTGGGAAGTGGTACAGGACACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAAcc                                                         >  2:1078547/1‑140 (MQ=255)                                                                            cGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCGCGGCTGGGAAGTGGTACAGGACACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAAccc                                                        >  2:326642/1‑140 (MQ=255)                                                                                aCGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCGCGGCTGGGAAGTGGTACAGGACACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGc                                                    <  2:24259/140‑1 (MQ=255)                                                                                 cGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCGCGGCTGGGAAGTGGTACAGGACACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGCa                                                   >  1:174049/1‑140 (MQ=255)                                                                                   gATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCGCGGCTGGGAAGTGGTACAGGACACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGCAGa                                                 >  1:1355524/1‑140 (MQ=255)                                                                                        gAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCGCGGCTGGGAAGTGGTACAGGACACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGCAGATGAAg                                            <  2:145067/140‑1 (MQ=255)                                                                                              tGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCGCGGCTGGGAAGTGGTACAGGACACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGCAGATGAAGCCGTCg                                      >  2:194150/1‑140 (MQ=255)                                                                                                aCGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCGCGGCTGGGAAGTGGTACAGGACACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGCAGATGAAGCCGTCGAg                                    >  2:967213/1‑140 (MQ=255)                                                                                                aCGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCGCGGCTGGGAAGTGGTACAGGACACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGCAGATGAAGCCGTCGAg                                    >  1:835171/1‑140 (MQ=255)                                                                                                  gATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCGCGGCTGGGAAGTGGTACAGGACACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGCAGATGAAGCCGTCGAGCa                                  <  2:1355524/140‑1 (MQ=255)                                                                                                   aTACCGTTCGCCTGACCATGCAACACGCGCAGCAGCGCGGCTGGGAAGTGGTACAGGACACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGCAGATGAAGCCGTCGAGCaa                                 >  2:1346887/1‑140 (MQ=255)                                                                                                   aTACCGTTCGCCTGACCATGCAACACGCGCAGCAGCGCGGCTGGGAAGTGGTACAGGACACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGCAGATGAAGCCGTCGAGCaa                                 >  2:1338204/1‑140 (MQ=255)                                                                                                   aTACCGTTCGCCTGACCATGCAACACGCGCAGCAGCGCGGCTGGGAAGTGGTACAGGACACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGCAGATGAAGCCGTCGAGCaa                                 >  1:872660/1‑140 (MQ=255)                                                                                                        gTTCGCCTGACCATGCAACACGCGCAGCAGCGCGGCTGGGAAGTGGTACAGGACACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGCAGATGAAGCCGTCGAGCAAATGCg                            <  1:803911/140‑1 (MQ=255)                                                                                                         ttCGCCTGACCATGCAACACGCGCAGCAGCGCGGCTGGGAAGTGGTACAGGACACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGCAGATGAAGCCGTCGAGCAAATGCGt                           >  2:1227292/1‑140 (MQ=255)                                                                                                         ttCGCCTGACCATGCAACACGCGCAGCAGCGCGGCTGGGAAGTGGTACAGGACACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGCAGATGAAGCCGTCGAGCAAATGCGt                           >  1:86256/1‑140 (MQ=255)                                                                                                          tCGCCTGACCATGCAACACGCGCAGCAGCGCGGCTGGGAAGTGGTACAGGACACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGCAGATGAAGCCGTCGAGCAAATGCGTg                          >  1:868352/1‑140 (MQ=255)                                                                                                           cGCCTGACCATGCAACACGCGCAGCAGCGCGGCTGGGAAGTGGTACAGGACACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGCAGATGAAGCCGTCGAGCAAATGCGTGa                         >  2:549565/1‑140 (MQ=255)                                                                                                             ccTGACCATGCAACACGCGCAGCAGCGCGGCTGGGAAGTGGTACAGGACACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGCAGATGAAGCCGTCGAGCAAATGCGTGaaa                       <  1:1030028/140‑1 (MQ=255)                                                                                                              cTGACCATGCAACACGCGCAGCAGCGCGGCTGGGAAGTGGTACAGGACACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGCAGATGAAGCCGTCGAGCAAATGCGTGAAAt                      >  1:1282839/1‑140 (MQ=255)                                                                                                              cTGACCATGCAACACGCGCAGCAGCGCGGCTGGGAAGTGGTACAGGACACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGCAGATGAAGCCGTCGAGCAAATGCGTGAAAt                      >  1:363869/1‑140 (MQ=255)                                                                                                               tGACCATGCAACACGCGCAGCAGCGCGGCTGGGAAGTGGTACAGGACACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGCAGATGAAGCCGTCGAGCAAATGCGTGAAATg                     <  1:194150/140‑1 (MQ=255)                                                                                                                 aCCATGCAACACGCGCAGCAGCGCGGCTGGGAAGTGGTACAGGACACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGCAGATGAAGCCGTCGAGCAAATGCGTGAAATggg                   >  2:60361/1‑140 (MQ=255)                                                                                                                   cATGCAACACGCGCAGCAGCGCGGCTGGGAAGTGGTACAGGACACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGCAGATGAAGCCGTCGAGCAAATGCGTGAAATGGGCg                 <  2:1357375/140‑1 (MQ=255)                                                                                                                     tGCAACACGCGCAGCAGCGCGGCTGGGAAGTGGTACAGGACACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGCAGATGAAGCCGTCGAGCAAATGCGTGAAATGGGCGTa               >  2:1352/1‑140 (MQ=255)                                                                                                                      gCAACACGCGCAGCAGCGCGGCTGGGAAGTGGTACAGGACACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGCAGATGAAGCCGTCGAGCAAATGCGTGAAATGGGCGTaa              >  2:1294340/1‑140 (MQ=255)                                                                                                                       cAACACGCGCAGCAGCGCGGCTGGGAAGTGGTACAGGACACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGCAGATGAAGCCGTCGAGCAAATGCGTGAAATGGGCGTAAc             >  2:719326/1‑140 (MQ=255)                                                                                                                       cAACACGCGCAGCAGCGCGGCTGGGAAGTGGTACAGGACACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGCAGATGAAGCCGTCGAGCAAATGCGTGAAATGGGCGTAAc             >  2:464797/1‑140 (MQ=255)                                                                                                                         acacGCGCAGCAGCGCGGCTGGGAAGTGGTACAGGACACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGCAGATGAAGCCGTCGAGCAAATGCGTGAAATGGGCGTAAccc           <  1:1338204/140‑1 (MQ=255)                                                                                                                            cgcgCAGCAGCGCGGCTGGGAAGTGGTACAGGACACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGCAGATGAAGCCGTCGAGCAAATGCGTGAAATGGGCGTAACCCCGa        >  1:377288/1‑140 (MQ=255)                                                                                                                            cgcgCAGCAGCGCGGCTGGGAAGTGGTACAGGACACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGCAGATGAAGCCGTCGAGCAAATGCGTGAAATGGGCGTAACCCCGa        >  1:603614/1‑140 (MQ=255)                                                                                                                             gcgcAGCAGCGCGGCTGGGAAGTGGTACAGGACACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGCAGATGAAGCCGTCGAGCAAATGCGTGAAATGGGCGTAACCCCGAc       <  2:835171/140‑1 (MQ=255)                                                                                                                             gcgcAGCAGCGCGGCTGGGAAGTGGTACAGGACACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGCAGATGAAGCCGTCGAGCAAATGCGTGAAATGGGCGTAACCCCGAc       <  1:577890/140‑1 (MQ=255)                                                                                                                               gcagcagcgCGGCTGGGAAGTGGTACAGGACACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGCAGATGAAGCCGTCGAGCAAATGCGTGAAATGGGCGTAACCCCGACGc     >  1:295028/1‑140 (MQ=255)                                                                                                                                 agcagcgCGGCTGGGAAGTGGTACAGGACACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGCAGATGAAGCCGTCGAGCAAATGCGTGAAATGGGCGTAACCCCGACGCAc   >  2:607143/1‑140 (MQ=255)                                                                                                                                  gcagcgCGGCTGGGAAGTGGTACAGGACACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGCAGATGAAGCCGTCGAGCAAATGCGTGAAATGGGCGTAACCCCGACGCACg  <  1:275171/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                       TGCGGTGATTTACATGCCGAAAGGTTCTGCTCAGGAACGCGTTGACGCCATTCTGAACCTCGGTGCCGAGTGCATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGACACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCCTGGCAGATGAAGCCGTCGAGCAAATGCGTGAAATGGGCGTAACCCCGACGCACG  >  NC_000913/3007920‑3008188

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑