Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	845,350	(T)8→7	coding (131/2226 nt)	ybiO ←	moderate conductance mechanosensitive channel

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	845,343	0	T	.	100.0%	295.4 / NA	68	coding (138/2226 nt)	ybiO	moderate conductance mechanosensitive channel
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (36/32);  total (36/32)

GCTCTTCGACCAGCGTCGGCGGCACTATTTTTGGTACCGGTTCAGCAGGGGGCGTAGCGGCAACGGTGCGCAACTGGTCGATCAACTCTTTACGCGAGGTGTCATTATCCAGCACATCCGCCAGTGCGCCATAGGCCGCTTTTTTTTGTTCGATATCCGGTTCCGGGGCCGGTTCAGTCGTTGAGTCCGTTGTCGTTGTGGTTGTAACGCCGGGTATGGATACCGCGTGGGCAGGTGCGCCCAGCAGGCAGAAGAGGATGAACAGGATCCA  >  NC_000913/845204‑845474                                                                                                                                            |                                                                                                                                    gCTCTTCGACCAGCGTCGGCGGCACTATTTTTGGTACCGGTTCAGCAGGGGGCGTAGCGGCAACGGTGCGCAACTGGTCGATCAACTCTTTACGCGAGGTGTCATTATCCAGCGCATCCGCCAGTGCGCCATAGGCCGCt                                                                                                                                     <  1:810820/140‑1 (MQ=255)      tCGACCAGCGTCGGCGGCACTATTTTTGGTACCGGTTCAGCAGGGGGCGTAGCGGCAACGGTGCGCAACTGGTCGATCAACTCTTTACGCGAGGTGTCATTATCCAGCGCATCCGCCAGTGCGCCATAGGCCGCtttttt                                                                                                                                >  2:922745/1‑140 (MQ=255)      tCGACCAGCGTCGGCGGCACTATTTTTGGTACCGGTTCAGCAGGGGGCGTAGCGGCAACGGTGCGCAACTGGTCGATCAACTCTTTACGCGAGGTGTCATTATCCAGCGCATCCGCCAGTGCGCCATAGGCCGCtttttt                                                                                                                                >  2:585407/1‑140 (MQ=255)        gACCAGCGTCGGCGGCACTATTTTTGGTACCGGTTCAGCAGGGGGCGTAGCGGCAACGGTGCGCAACTGGTCGATCAACTCTTTACGCGAGGTGTCATTATCCAGCGCATCCGCCAGTGCGCCATAGGCCGCtttttttg                                                                                                                              <  1:575067/140‑2 (MQ=255)              cGTCGGCGGCACTATTTTTGGTACCGGTTCAGCAGGGGGCGTAGCGGCAACGGTGCGCAACTGGTCGATCAACTCTTTACGCGAGGTGTCATTATCCAGCGCATCCGCCAGTGCGCCATAGGCCGC‑TTTTTTTGTTCGat                                                                                                                       >  1:83366/1‑140 (MQ=255)                   gcggcACTATTTTTGGTACCGGTTCAGCAGGGGGCGTAGCGGCAACGGTGCGCAACTGGTCGATCAACTCTTTACGCGAGGTGTCATTATCCAGCGCATCCGCCAGTGCGCCATAGGCCGC‑TTTTTTTGTTCGATATCCg                                                                                                                  <  1:699444/140‑1 (MQ=255)                        aCTATTTTTGGTACCGGTTCAGCAGGGGGCGTAGCGGCAACGGTGCGCAACTGGTCGATCAACTCTTTACGCGAGGTGTCATTATCCAGCGCATCCGCCAGTGCGCCATAGGCCGC‑TTTTTTTGTTCGATATCCGGTTcc                                                                                                             >  1:751781/1‑140 (MQ=255)                        aCTATTTTTGGTACCGGTTCAGCAGGGGGCGTAGCGGCAACGGTGCGCAACTGGTCGATCAACTCTTTACGCGAGGTGTCATTATCCAGCGCATCCGCCAGTGCGCCATAGGCCGC‑TTTTTTTGTTCGATATCCGGTTcc                                                                                                             >  1:1200078/1‑140 (MQ=255)                           aTTTTTGGTACCGGTTCAGCAGGGGGCGTAGCGGCAACGGTGCGCAACTGGTCGATCAACTCTTTACGCGAGGTGTCATTATCCAGCGCATCCGCCAGTGCGCCATAGGCCGC‑TTTTTTTGTTCGATATCCGGTTCCggg                                                                                                          <  2:528006/140‑1 (MQ=255)                                  gTACCGGTTCAGCAGGGGGCGTAGCGGCAACGGTGCGCAACTGGTCGATCAACTCTTTACGCGAGGTGTCATTATCCAGCGCATCCGCCAGTGCGCCATAGGCCGC‑TTTTTTTGTTCGATATCCGGTTCCGGGGCCGGtt                                                                                                   <  2:751781/140‑1 (MQ=255)                                     ccGGTTCTGCAGGGGGCGTGGCGGCGGCGGTGCCCAGCTGTTCGATAAACTCTTTACGCGAGGTGTCATTATCCAGCGCAGCCGCCAGTGCGCCATAGGCCGC‑TTTTTTTGTTCGATATCCGGTTCCGGGGCCGGTTCAg                                                                                                <  2:1200078/140‑1 (MQ=255)                                      cGGTTCAGCAGGGGGCGTAGCGGCAACGGTGCGCAACTGGTCGATCAACTCTTTACGCGAGGTGTCATTATCCAGCGCATCCGCCAGTGCGCCATAGGCCGC‑TTTTTTTGTTCGATATCCGGTTCCGGGGCCGGTTCAGt                                                                                               >  2:1138285/1‑140 (MQ=255)                                      cGGTGCAGCAGGGGGCGTAGCGGCAATGGTGCGAAGCTGGTCGTTCAAGCCTTTACGCGGGGTGTCTTTATCCAGGGCACCCGCCAATGCGCCATAGGCCGC‑TTTTTTTGTTCGATATCTGGTTCCGCGACCGGGTCAga                                                                                               >  2:247233/1‑139 (MQ=255)                                       ggTTCAGCAGGGGGCGTAGCGGCAACGGTGCGCAACTGGTCGATCAACTCTTTACGCGAGGTGTCATTATCCAGCGCATCCGCCAGTGCGCCATAGGCCGC‑TTTTTTTGTTCGATATCCGGTTCCGGGGCCGGtt                                                                                                   <  2:606263/135‑1 (MQ=255)                                       ggTTCAGCAGGGGGCGTAGCGGCAACGGTGCGCAACTGGTCGATCAACTCTTTACGCGAGGTGTCATTATCCAGCGCATCCGCCAGTGCGCCATAGGCCGC‑TTTTTTTGTTCGATATCCGGTTCCGGGGCCGGtt                                                                                                   >  1:606263/1‑135 (MQ=255)                                        gTTCAGCAGGGGGCGTAGCGGCAACGGTGCGCAACTGGTCGATCAACTCTTTACGCGAGGTGTCATTATCCAGCGCATCCGCCAGTGCGCCATAGGCCGC‑TTTTTTTGTTCGATATCCGGTTCCGGGGc                                                                                                        >  1:322214/1‑129 (MQ=255)                                        gTTCAGCAGGGGGCGTAGCGGCAACGGTGCGCAACTGGTCGATCAACTCTTTACGCGAGGTGTCATTATCCAGCGCATCCGCCAGTGCGCCATAGGCCGC‑TTTTTTTGTTCGATATCCGGTTCCGGGGc                                                                                                        <  2:322214/129‑1 (MQ=255)                                        gTTCAGCAGGGGGCGTAGCGGCAACGGTGCGCAACTGGTCGATCAACTCTTTACGCGAGGTGTCATTATCCAGCGCATCCGCCAGTGCGCCATAGGCCGC‑TTTTTTTGTTCGATATCCGGTTCCGGGGCCGGTTCAGTCg                                                                                             >  1:999420/1‑140 (MQ=255)                                          tCAGCAGGGGGCGTAGCGGCAACGGTGCGCAACTGGTCGATCAACTCTTTACGCGAGGTGTCATTATCCAGCGCATCCGCCAGTGCGCCATAGGCCGC‑TTTTTTTGTTCGATATCCGGTTCCGGGGCCGGTTCAGTCGtt                                                                                           <  2:782027/140‑1 (MQ=255)                                           cagcagGGGGCGTAGCGGCAACGGTGCGCAACTGGTCGATCAACTCTTTACGCGAGGTGTCATTATCCAGCGCATCCGCCAGTGCGCCATAGGCCGC‑TTTTTTTGTTCGATATCCGGTTCCGGGGCCGGTTCAGTCGTTg                                                                                          <  2:39040/140‑1 (MQ=255)                                              cagGGGGCGTAGCGGCAACGGTGCGCAACTGGTCGATCAACTCTTTACGCGAGGTGTCATTATCCAGCGCATCCGCCAGTGCGCCATAGGCCGC‑TTTTTTTGTTCGATATCCGGTTCCGGGGCCGGTTCAGTCGTTGAGt                                                                                       >  2:860039/1‑140 (MQ=255)                                               agGGGGCGTAGCGGCAACGGTGCGCAACTGGTCGATCAACTCTTTACGCGAGGTGTCATTATCCAGCGCATCCGCCAGTGCGCCATAGGCCGC‑TTTTTTTGTTCGATATCCGGTTCCGGGGCCGGTTCAGTCGTTGAGTc                                                                                      <  2:1008065/140‑1 (MQ=255)                                                   ggCGTAGCGGCAACGGTGCGCAACTGGTCGATCAACTCTTTACGCGAGGTGTCATTATCCAGCGCATCCGCCAGTGCGCCATAGGCCGC‑TTTTTTTGTTCGATATCCGGTTCCGGGGCCGGTTCAGTCGTTGAGTCcgtt                                                                                  <  1:922745/140‑1 (MQ=255)                                                   ggCGTAGCGGCAACGGTGCGCAACTGGTCGATCAACTCTTTACGCGAGGTGTCATTATCCAGCGCATCCGCCAGTGCGCCATAGGCCGC‑TTTTTTTGTTCGATATCCGGTTCCGGGGCCGGTTCAGTCGTTGAGTCcgtt                                                                                  >  1:91929/1‑140 (MQ=255)                                                   ggCGTAGCGGCAACGGTGCGCAACTGGTCGATCAACTCTTTACGCGAGGTGTCATTATCCAGCGCATCCGCCAGTGCGCCATAGGCCGC‑TTTTTTTGTTCGATATCCGGTTCCGGGGCCGGTTCAGTCGTTGAGTCcgtt                                                                                  >  2:543162/1‑140 (MQ=255)                                                   ggCGTAGCGGCAACGGTGCGCAACTGGTCGATCAACTCTTTACGCGAGGTGTCATTATCCAGCGCATCCGCCAGTGCGCCATAGGCCGC‑TTTTTTTGTTCGATATCCGGTTCCGGGGCCGGTTCAGTCGTTGAGTCcgtt                                                                                  >  2:720132/1‑140 (MQ=255)                                                   ggCGTAGCGGCAACGGTGCGCAACTGGTCGATCAACTCTTTACGCGAGGTGTCATTATCCAGCGCATCCGCCAGTGCGCCATAGGCCGC‑TTTTTTTGTTCGATATCCGGTTCCGGGGCCGGTTCAGTCGTTGAGTCcgtt                                                                                  <  1:585407/140‑1 (MQ=255)                                                   ggCGTAGCGGCAACGGTGCGCAACTGGTCGATCAACTCTTTACGCGAGGTGTCATTATCCAGCGCATCCGCCAGTGCGCCATAGGCCGC‑TTTTTTTGTTCGATATCCGGTTCCGGGGCCGGTTCAGTCGTTGAGTCcgtt                                                                                  >  2:680557/1‑140 (MQ=255)                                                   ggCGTAGCGGCAACGGTGCGCAACTGGTCGATCAACTCTTTACGCGAGGTGTCATTATCCAGCGCATCCGCCAGTGCGCCATAGGCCGC‑TTTTTTTGTTCGATATCCGGTTCCGGGGCCGGTTCAGTCGTTGAGTCcgtt                                                                                  >  1:452318/1‑140 (MQ=255)                                                      gTAGCGGCAACGGTGCGCAACTGGTCGATCAACTCTTTACGCGAGGTGTCATTATCCAGCGCATCCGCCAGTGCGCCATAGGCCGC‑TTTTTTTGTTCGATATCCGGTTCCGGGGCCGGTTCAGTCGTTGAGTCcgttgtc                                                                               >  2:499138/1‑140 (MQ=255)                                                      gTAGCGGCAACGGTGCGCAACTGGTCGATCAACTCTTTACGCGAGGTGTCATTATCCAGCGCATCCGCCAGTGCGCCATAGGCCGC‑TTTTTTTGTTCGATATCCGGTTCCGGGGCCGGTTCAGTCGTTGAGTCcgttgtc                                                                               >  2:836055/1‑140 (MQ=255)                                                      gTAGCGGCAACGGTGCGCAACTGGTCGATCAACTCTTTACGCGAGGTGTCATTATCCAGCGCATCCGCCAGTGCGCCATAGGCCGC‑TTTTTTTGTTCGATATCCGGTTCCGGGGCCGGTTCAGTCGTTGAGTCcgttgtc                                                                               >  1:488447/1‑140 (MQ=255)                                                        aGCGGCAACGGTGCGCAACTGGTCGATCAACTCTTTACGCGAGGTGTCATTATCCAGCGCATCCGCCAGTGCGCCATAGGCCGC‑TTTTTTTGTTCGATATCCGGTTCCGGGGCCGGTTCAGTCGTTGAGTCcgttgtcgt                                                                             >  2:323773/1‑140 (MQ=255)                                                         gCGGCAACGGTGCGCAACTGGTCGATCAACTCTTTACGCGAGGTGTCATTATCCAGCGCATCCGCCAGTGCGCCATAGGCCGC‑TTTTTTTGTTCGATATCCGGTTCCGGGGCCGGTTCAGTCGTTGAGTCcgttgtcgtt                                                                            <  1:1018904/140‑1 (MQ=255)                                                         gCGGCAACGGTGCGCAACTGGTCGATCAACTCTTTACGCGAGGTGTCATTATCCAGCGCATCCGCCAGTGCGCCATAGGCCGC‑TTTTTTTGTTCGATATCCGGTTCCGGGGCCGGTTCAGTCGTTGAGTCcgttgtcgtt                                                                            <  1:860039/140‑1 (MQ=255)                                                                      gcAACTGGTCGATCAACTCTTTACGCGAGGTGTCATTATCCAGCGCATCCGCCAGTGCGCCATAGGCCGC‑TTTTTTTGTTCGATATCCGGTTCCGGGGCTGGTTCAGTCGTTGAGTCCGTTGTCGTTGTGGTTGTAACGc                                                               <  1:74490/140‑1 (MQ=255)                                                                      gcAACTGGTCGATCAACTCTTTACGCGAGGTGTCATTATCCAGCGCATCCGCCAGTGCGCCATAGGCCGC‑TTTTTTTGTTCGATATCCGGTTCCGGGGCCGGTTCAGTCGTTGAGTCCGTTGTCGTTGTGGTTGTAACGc                                                               <  2:1027204/140‑1 (MQ=255)                                                                        aaCTGGTCGATCAACTCTTTACGCGAGGTGTCATTATCCAGCGCATCCGCCAGTGCGCCATAGGCCGC‑TTTTTTTGTTCGATATCCGGTTCCGGGGCCGGTTCAGTCGTTGAGTCCGTTGTCGTTGTGGTTGTAACGCCg                                                             <  2:83366/140‑1 (MQ=255)                                                                        aaCTGGTCGATCAACTCTTTACGCGAGGTGTCATTATCCAGCGCATCCGCCAGTGCGCCATAGGCCGC‑TTTTTTTGTTCGATATCCGGTTCCGGGGCCGGTTCAGTCGTTGAGTCCGTTGTCGTTGTGGTTGTAACGCCg                                                             <  1:1015569/140‑1 (MQ=255)                                                                             gTCGATCAACTCTTTACGCGAGGTGTCATTATCCAGCGCATCCGCCAGTGCGCCATAGGCCGC‑TTTTTTTGTTCGATATCCGGTTCCGGGGCCGGTTCAGTCGTTGAGTCCGTTGTCGTTGTGGTTGTAACGCCGGGTAt                                                        >  1:1101653/1‑140 (MQ=255)                                                                                  tCAACTCTTTACGCGAGGTGTCATTATCCAGCGCATCCGCCAGTGCGCCATAGGCCGC‑TTTTTTTGTTCGATATCCGGTTCCGGGGCCGGTTCAGTCGTTGAGTCCGTTGTCGTTGTGGTTGTAACGCCGGGTATGGATa                                                   <  2:1101653/140‑1 (MQ=255)                                                                                     aCTCTTTACGCGAGGTGTCATTATCCAGCGCATCCGCCAGTGCGCCATAGGCCGC‑TTTTTTTGTTCGATATCCGGTTCCGGGGCCGGTTCAGTCGTTGAGTCCGTTGTCGTTGTGGTTGTAACGCCGGGTATGGATACcg                                                >  1:740136/1‑140 (MQ=255)                                                                                         tttACGCGAGGTGTCATTATCCAGCGCATCCGCCAGTGCGCCATAGGCCGC‑TTTTTTTGTTCGATATCCGGTTCCGGGGCCGGTTCAGTCGTTGAGTCCGTTGTCGTTGTGGTTGTAACGCCGGGTATGGATACCGCGTg                                            <  1:1138285/140‑1 (MQ=255)                                                                                                        aTTATCCAGCGCATCCGCCAGTGCGCCATAGGCCGC‑TTTTTTTGTTCGATATCCGGTTCCGGGGCCGGTTCAGTCGTTGAGTCCGTTGTCGTTGTGGTTGTAACGCCGGGTATGGATACCGCGTGGGCAGGTGCGCCcag                             <  2:999420/140‑1 (MQ=255)                                                                                                            tCCAGCGCATCCGCCAGTGCGCCATAGGCCGC‑TTTTTTTGTTCGATATCCGGTTCCGGGGCCGGTTCAGTCGTTGAGTCCGTTGTCGTTGTGGTTGTAACGCCGGGTATGGATACCGCGTGGGCAGGTGCGCCCAgcagg                         >  1:629830/1‑140 (MQ=255)                                                                                                              cAGCGCATCCGCCAGTGCGCCATAGGCCGC‑TTTTTTTGTTCGATATCCGGTTCCGGGGCCGGTTCAGTCGTTGAGTCCGTTGTCGTTGTGGTTGTAACGCCGGGTATGGATACCGCGTGGGCAGGTGCGCCCAgcaggca                       >  2:980146/1‑140 (MQ=255)                                                                                                              cAGCGCATCCGCCAGTGCGCCATAGGCCGC‑TTTTTTTGTTCGATATCCGGTTCCGGGGCCGGTTCAGTCGTTGAGTCCGTTGTCGTTGTGGTTGTAACGCCGGGTATGGATACCGCGTGGGCAGGTGCGCCCAgcaggca                       >  1:1082624/1‑140 (MQ=255)                                                                                                              cAGCGCATCCGCCAGTGCGCCATAGGCCGC‑TTTTTTTGTTCGATATCCGGTTCCGGGGCCGGTTCAGTCGTTGAGTCCGTTGTCGTTGTGGTTGTAACGCCGGGTATGGATACCGCGTGGGCAGGTGCGCCCAgcaggca                       <  1:139219/140‑1 (MQ=255)                                                                                                              cAGCGCATCCGCCAGTGCGCCATAGGCCGC‑TTTTTTTGTTCGATATCCGGTTCCGGGGCCGGTTCAGTCGTTGAGTCCGTTGTCGTTGTGGTTGTAACGCCGGGTATGGATACCGCGTGGGCAGGTGCGCCCAgcaggca                       >  1:533193/1‑140 (MQ=255)                                                                                                                gcgcaTCCGCCAGTGCGCCATAGGCCGC‑TTTTTTTGTTCGATATCCGGTTCCGGGGCCGGTTCAGTCGTTGAGTCCGTTGTCGTTGTGGTTGTAACGCCGGGTATGGATACCGCGTGGGCAGGTgcgc                                 <  1:1053706/125‑1 (MQ=255)                                                                                                                gcgcaTCCGCCAGTGCGCCATAGGCCGC‑TTTTTTTGTTCGATATCCGGTTCCGGGGCCGGTTCAGTCGTTGAGTCCGTTGTCGTTGTGGTTGTAACGCCGGGTATGGATACCGCGTGGGCAGGTGCGCCCAGCAGGCaga                     >  2:687873/4‑140 (MQ=255)                                                                                                                gcgcaTCCGCCAGTGCGCCATAGGCCGC‑TTTTTTTGTTCGATATCCGGTTCCGGGGCCGGTTCAGTCGTTGAGTCCGTTGTAGTTGTGGTTGTAACGCCGGGTATGGATACCGCGTGGGCAGGTgcgc                                 >  2:1053706/4‑128 (MQ=255)                                                                                                                 cgcaTCCGCCAGTGCGCCATAGGCCGC‑TTTTTTTGTTCGATATCCGGTTCCGGGGCCGGTTCAGTCGTTGAGTCCGTTGTCGTTGTGGTTGTAACGCCGGGTATGGATACCGCGTGGGCAGGTGCGCCCAGCAGGCagaa                    >  1:184552/3‑140 (MQ=255)                                                                                                                 cgcaTCCGCCAGTGCGCCATAGGCCGC‑TTTTTTTGTTCGATATCCGGTTCCGGGGCCGGTTCAGTCGTTGAGTCCGTTGTCGTTGTGGTTGTAACGCCGGGTATGGATACCGCGTGGGCAGGTGCGCCCAGCAGGCagaa                    >  1:869879/3‑140 (MQ=255)                                                                                                                 cgcaTCCGCCAGTGCGCCATAGGCCGC‑TTTTTTTGTTCGATATCCGGTTCCGGGGCCGGTTCAGTCGTTGAGTCCGTTGTCGTTGTGGTTGTAACGCCGGGTATGGATACCGCGTGGGCAGGTGCGCCCAGCAGGCagaa                    >  2:644434/3‑140 (MQ=255)                                                                                                                 cgcaTCCGCCAGTGCGCCATAGGCCGC‑TTTTTTTGTTCGATATCCGGTTCCGGGGCCGGTTCAGTCGTTGAGTCCGTTGTCGTTGTGGTTGTAACGCCGGGTATGGATACCGCGTGGGCAGGTGCGCCCAGCAGGCagaa                    >  1:786236/3‑140 (MQ=255)                                                                                                                   caTCCGCCAGTGCGCCATAGGCCGC‑TTTTTTTGTTCGATATCCGGTTCCGGGGCCGGTTCAGTCGTTGAGTCCGTTGTCGTTGTGGTTGTAACGCCGGGTATGGATACCGCGTGGGCAGGTGCGCCCAGCAGGCagaaga                  >  1:1042993/1‑140 (MQ=255)                                                                                                                    aTCCGCCAGTGCGCCATAGGCCGC‑TTTTTTTGTTCGATATCCGGTTCCGGGGCCGGTTCAGTCGTTGAGTCCGTTGTCGTTGTGGTTGTAACGCCGGGTATGGATACCGCGTGGGCAGGTGCGCCca                              <  1:485288/127‑1 (MQ=255)                                                                                                                    aTCCGCCAGTGCGCCATAGGCCGC‑TTTTTTTGTTCGATATCCGGTTCCGGGGCCGGTTCAGTCGTTGAGTCCGTTGTCGTTGTGGTTGTAACGCCGGGTATGGATACCGCGTGGGCAGGTGCGCCca                              >  2:485288/1‑127 (MQ=255)                                                                                                                    aTCCGCCAGTGCGCCATAGGCCGC‑TTTTTTTGTTCGATATCCGGTTCCGGGGCCGGTTCAGTCGTTGAGTCCGTTGTCGTTGTGGTTGTAACGCCGGGTATGGATACCGCGTGGGCAGGTGCGCCCAGCAGGCAGAagag                 >  1:186193/1‑140 (MQ=255)                                                                                                                     tCCGCCAGTGCGCCATAGGCCGC‑TTTTTTTGTTCGATATCCGGTTCCGGGGCCGGTTCAGTCGTTGAGTCCGTTGTCGTTGTGGTTGTAACGCCGGGTATGGATACCGCGTGGGCAGGTGCGCCCAGCAGGCAGAAGAgg                <  2:488447/140‑1 (MQ=255)                                                                                                                      ccGCCAGTGCGCCATAGGCCGC‑TTTTTTTGTTCGATATCCGGTTCCGGGGCCGGTTCAGTCGTTGAGTCCGTTGTCGTTGTGGTTGTAACGCCGGGTATGGATACCGCGTGGGCAGGTGCGCCCAGCAGGCAGAAGAGGa               >  1:310060/1‑140 (MQ=255)                                                                                                                          cAGTGCGCCATAGGCCGC‑TTTTTTTGTTCGATATCCGGTTCCGGGGCCGGTTCAGTCGTTGAGTCCGTTGTCGTTGTGGTTGTAACGCCGGGTATGGATACCGCGTGGGCAGGTGCGCCCAGCAGGCAGAAGAGGATGaa           <  1:644434/140‑1 (MQ=255)                                                                                                                             tGCGCCATAGGCCGC‑TTTTTTTGTTCGATATCCGGTTCCGGGGCCGGTTCAGTCGTTGAGTCCGTTGTCGTTGTGGTTGTAACGCCGGGTATGGATACCGCGTGGGCAGGTGCGCCCAGCAGGCAGAAGAGGATGAACAg        >  1:942199/1‑140 (MQ=255)                                                                                                                             tGCGCCATAGGCCGC‑TTTTTTTGTTCGATATCCGGTTCCGGGGCCGGTTCAGTCGTTGAGTCCGTTGTCGTTGTGGTTGTAACGCCGGGTATGGATACCGCGTGGGCAGGTGCGCCCAGCAGGCAGAAGAGGATGAACAg        >  1:1201738/1‑140 (MQ=255)                                                                                                                              gcgcCATAGGCCGC‑‑TTTTTTGTTCGATATCCGGTTCCGGGGCCGGTTCAGTCGTTGAGTCCGTTGTCGTTGTGGTTGTAACGCCGGGTATGGATACCGCGTGGGCAGGTGCGCCCAGCAGGCAGAAGAGGATGAACAGGa      >  1:698032/1‑140 (MQ=255)                                                                                                                                 ccATAGGCCGC‑TTTTTTTGTTCGATATCCGGTTCCGGGGCCGGTTCAGTCGTTGAGTCCGTTGTCGTTGTGGTTGTAACGCCGGGTATGGATACCGCGTGGGCAGGTGCGCCCAGCAGGCAGAAGAGGATGAACAGGATc    <  2:533193/140‑1 (MQ=255)                                                                                                                                 ccATAGGCCGC‑TTTTTTTGTTCGATATCCGGTTCCGGGGCCGGTTCAGTCGTTGAGTCCGTTGTCGTTGTGGTTGTAACGCCGGGTATGGATACCGCGTGGGCAGGTGCGCCCAGCAGGCAGAAGAGGATGAACAGGATc    <  2:786236/140‑1 (MQ=255)                                                                                                                                   aTAGGCCGC‑TTTTTTTGTTCGATATCCGGTTCCGGGGCCGGTTCAGTCGTTGAGTCCGTTGTCGTTGTGGTTGTAACGCCGGGTATGGATACCGCGTGGGCAGGTGCGCCCAGCAGGCAGAAGAGGATGAACAGGATCCa  <  2:326940/140‑1 (MQ=255)                                                                                                                                   aTAGGCCGC‑TTTTTTTGTTCGATATCCGGTTCCGGGGCCGGTTCAGTCGTTGAGTCCGTTGTCGTTGTGGTTGTAACGCCGGGTATGGATACCGCGTGGGCAGGTGCGCCCAGCAGGCAGAAGAGGATGAACAGGATCCa  <  1:738317/140‑1 (MQ=255)                                                                                                                                   aTAGGCCGC‑TTTTTTTGTTCGATATCCGGTTCCGGGGCCGGTTCAGTCGTTGAGTCCGTTGTCGTTGTGGTTGTAACGCCGGGTATGGATACCGCGTGGGCAGGTGCGCCCAGCAGGCAGAAGAGGATGAACAGGATCCa  <  2:310060/140‑1 (MQ=255)                                                                                                                                   aTAGGCCGC‑TTTTTTTGTTCGATATCCGGTTCCGGGGCCGGTTCAGTCGTTGAGTCCGTTGTCGTTGTGGTTGTAACGCCGGGTATGGATACCGCGTGGGCAGGTGCGCCCAGCAGGCAGAAGAGGATGAACAGGATCCa  <  2:258900/140‑1 (MQ=255)                                                                                                                                   aTAGGCCGC‑TTTTTTTGTTCGATATCCGGTTCCGGGGCCGGTTCAGTCGTTGAGTCCGTTGTCGTTGTGGTTGTAACGCCGGGTATGGATACCGCGTGGGCAGGTGCGCCCAGCAGGCAGAAGAGGATGAACAGGATCCa  <  2:1082624/140‑1 (MQ=255)                                                                                                                                            |                                                                                                                                    GCTCTTCGACCAGCGTCGGCGGCACTATTTTTGGTACCGGTTCAGCAGGGGGCGTAGCGGCAACGGTGCGCAACTGGTCGATCAACTCTTTACGCGAGGTGTCATTATCCAGCACATCCGCCAGTGCGCCATAGGCCGCTTTTTTTTGTTCGATATCCGGTTCCGGGGCCGGTTCAGTCGTTGAGTCCGTTGTCGTTGTGGTTGTAACGCCGGGTATGGATACCGCGTGGGCAGGTGCGCCCAGCAGGCAGAAGAGGATGAACAGGATCCA  >  NC_000913/845204‑845474

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑