Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	34,120	(T)9→8	intergenic (+82/‑180)	carB → / → caiF	carbamoyl phosphate synthetase subunit beta/DNA‑binding transcriptional activator CaiF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	34,112	0	T	.	100.0%	339.7 / NA	78	intergenic (+74/‑188)	carB/caiF	carbamoyl phosphate synthetase subunit beta/DNA‑binding transcriptional activator CaiF
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (42/36);  total (42/36)

ATGCCGATGCGACTGAAAAAGTAATTTCGGTGCAGGAAATGCACGCACAGATCAAATAATAGCGTGTCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCGTTTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACGGTGAAGTTGCATACTATCGATATATCCACAATTTTAATATGGCCTTGTTTAAT  >  NC_000913/33980‑34247                                                                                                                                     |                                                                                                                                        aTGCCGATGCGACTGAAAAAGTAATTTCGGTGCAGGAAATGCACGCACAGATCAAATAATAGCGTGTCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCGtttttttt                                                                                                                                  <  2:114248/140‑1 (MQ=255)  tGCCGATGCGACTGAAAAAGTAATTTCGGTGCAGGAAATGCACGCACAGATCAAATAATAGCGTGTCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCGttttttttg                                                                                                                                 <  1:160374/140‑2 (MQ=255)       aTGCGACTGAAAAAGTAATTTCGGTGCAGGAAATGCACGCACAGATCAAATAATAGCGTGTCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCGttttttttg                                                                                                                                 <  2:1069186/135‑2 (MQ=255)       aTGCGACTGAAAAAGTAATTTCGGTGCAGGAAATGCACGCACAGATCAAATAATAGCGTGTCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCGttttttttg                                                                                                                                 >  1:1069186/1‑134 (MQ=255)       aTGCGACTGAAAAAGTAATTTCGGTGCAGGAAATGCACGCACAGATCAAATAATAGCGTGTCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCAt                                                                                                                           <  2:1044265/140‑1 (MQ=255)          cGACTGAAAAAGTAATTTCGGTGCAGGAAATGCACGCACAGATCAAATAATAGCGTGTCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTaa                                                                                                                        >  2:1002031/1‑140 (MQ=255)                  aaaGTAATTTCGGTGCAGGAAATGCACGCACAGATCAAATAATAGCGTGTCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCt                                                                                                                <  2:1090746/140‑1 (MQ=255)                              gTGCAGGAAATGCACGCACAGATCAAATAATAGCGTGTCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCg                                                                                                    >  2:251356/1‑140 (MQ=255)                               tGCAGGAAATGCACGCACAGATCAAATAATAGCGTGTCATGGCAGATATTTTTCATCCGCTAATGTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGa                                                                                                   >  2:1118971/1‑140 (MQ=255)                                 cAGGAAATGCACGCACAGATCAAATAATAGCGTGTCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGata                                                                                                 <  1:455654/140‑1 (MQ=255)                                            cgcacAGATCAAATAATAGCGTGTCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGAc                                                                                      <  1:159945/140‑1 (MQ=255)                                              cacaGATCAAATAATAGCGTGTCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACAt                                                                                    >  1:889244/1‑140 (MQ=255)                                              cacaGATCAAATAATAGCGTGTCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACAt                                                                                    <  2:472966/140‑1 (MQ=255)                                                  gATCAAATAATAGCGTGTCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGc                                                                                >  1:212960/1‑140 (MQ=255)                                                   aTCAAATAATAGCGTGTCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAg                                                                                 <  1:874932/138‑1 (MQ=255)                                                   aTCAAATAATAGCGTGTCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAg                                                                                 >  2:874932/1‑138 (MQ=255)                                                   aTCAAATAATAGCGTGTCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCa                                                                               >  2:640983/1‑140 (MQ=255)                                                   aTCAAATAATAGCGTGTCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCa                                                                               <  1:639235/140‑1 (MQ=255)                                                   aTCAAATAATAGCGTGTCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCa                                                                               >  1:33923/1‑140 (MQ=255)                                                   aTCAAATAATAGCGTGTCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCa                                                                               >  1:909411/1‑140 (MQ=255)                                                   aTCAAATAATAGCGTGTCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCa                                                                               >  2:713962/1‑140 (MQ=255)                                                       aataatAGCGTGTCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGAt                                                                           <  1:713962/140‑1 (MQ=255)                                                        ataataGCGTGTCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGAtt                                                                          <  2:889244/140‑1 (MQ=255)                                                           ataGCGTGTCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTAATAAC‑TAAGCAGGGATTAGc                                                                      >  1:484911/1‑140 (MQ=255)                                                             aGCGTGTCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGAt                                                                           <  2:970434/134‑1 (MQ=255)                                                             aGCGTGTCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGAt                                                                           >  1:970434/1‑134 (MQ=255)                                                             aGCGTGTCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCt                                                                     >  2:143563/1‑140 (MQ=255)                                                              gCGTGTCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCa                                                                               <  1:666800/129‑1 (MQ=255)                                                              gCGTGTCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCa                                                                               >  2:666800/1‑129 (MQ=255)                                                               cGTGTCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGTATTTAGCTca                                                                   >  2:739725/1‑140 (MQ=255)                                                               cGTGTCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTca                                                                   <  1:640983/140‑1 (MQ=255)                                                               cGTGTCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTca                                                                   <  2:97472/140‑1 (MQ=255)                                                               cGTGTCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTca                                                                   <  1:1118971/140‑1 (MQ=255)                                                               cGTGTCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTca                                                                   <  2:645315/140‑1 (MQ=255)                                                               cGTGTCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTc                                                                    >  1:189157/1‑139 (MQ=255)                                                               cGTGTCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTc                                                                    <  2:189157/139‑1 (MQ=255)                                                                gtgtCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTcac                                                                  >  1:1055476/1‑140 (MQ=255)                                                                 tgtCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTcaca                                                                 <  2:307873/140‑1 (MQ=255)                                                                 tgtCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTcaca                                                                 >  2:415128/1‑140 (MQ=255)                                                                 tgtCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTcaca                                                                 <  2:212960/140‑1 (MQ=255)                                                                 tgtCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTcaca                                                                 >  1:782044/1‑140 (MQ=255)                                                                 tgtCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTcaca                                                                 >  1:881219/1‑140 (MQ=255)                                                                  gtCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCacac                                                                >  2:142206/1‑140 (MQ=255)                                                                   tCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACt                                                               >  1:979096/1‑140 (MQ=255)                                                                      tGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTAt                                                            >  1:1125923/1‑140 (MQ=255)                                                                           gATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACg                                                       >  2:1074954/1‑140 (MQ=255)                                                                            atatTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACgg                                                      >  2:289986/1‑140 (MQ=255)                                                                              atTTTTCATCCGCTAATTTGTTCGAATAACTAATACGGTTCTATGATGAGGACCG‑TTTTTTTTGCACCTTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACGGTg                                                    >  2:1052544/1‑140 (MQ=255)                                                                              atTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACGGTg                                                    <  2:979096/140‑1 (MQ=255)                                                                                      tCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACGGTGAAGTTGCa                                            <  1:1002031/140‑1 (MQ=255)                                                                                      tCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACGGTGAAGTTGCa                                            <  1:143563/140‑1 (MQ=255)                                                                                      tCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACGGTGAAGTTGCa                                            <  1:251356/140‑1 (MQ=255)                                                                                      tCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACGGTGAAGTTGCa                                            <  2:1125923/140‑1 (MQ=255)                                                                                      tCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACGGTGAAGTTGCa                                            <  2:782044/140‑1 (MQ=255)                                                                                        cGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACGGTGAAGTTGCATa                                          <  2:931433/140‑1 (MQ=255)                                                                                          cTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACGGTGAAGTTGCATACt                                        >  1:196818/1‑140 (MQ=255)                                                                                          cTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACGGTGAAGTTGCATACt                                        >  2:702366/1‑140 (MQ=255)                                                                                                tGATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACGGTGAAGTTGCATACTATCGat                                  <  1:299465/140‑1 (MQ=255)                                                                                                 gATCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGTCATAAGCAGGATTTAGCTCACACTTATCGACGGTGAAGTTGCATACTATCGata                                 <  1:1073128/140‑1 (MQ=255)                                                                                                  aTCGAATAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACGGTGAAGTTGCATACTATCGatat                                >  1:797059/1‑140 (MQ=255)                                                                                                       aTAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACGGTGAAGTTGCATACTATCGATATATCca                           >  1:1011768/1‑140 (MQ=255)                                                                                                       aTAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACGGTGAAGTTGCATACTATCGATATATCca                           <  2:303391/140‑1 (MQ=255)                                                                                                       aTAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACGGTGAAGTTGCATACTATCGATATATCca                           <  1:582183/140‑1 (MQ=255)                                                                                                        tAACTAATACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACGGTGAAGTTGCATACTATCGATATATCcac                          <  1:739725/140‑1 (MQ=255)                                                                                                             aaTACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACGGTGAAGTTGCATACTATCGATATATCCACAAttt                     <  1:217031/140‑1 (MQ=255)                                                                                                              aTACGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACGGTGAAGTTGCATACTATCGATATATCCACAAtttt                    >  1:1102674/1‑140 (MQ=255)                                                                                                                 cGGTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACGGTGAAGTTGCATACTATCGATATATCCACAATTTTAat                 <  2:1026371/140‑1 (MQ=255)                                                                                                                  ggTTCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACGGTGAAGTTGCATACTATCGATATATCCACAATTTTAata                <  2:909411/140‑1 (MQ=255)                                                                                                                    ttCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACGGTGAAGTTGCATACTATCGATATATCCACAATTTTAATATg              >  2:755411/1‑140 (MQ=255)                                                                                                                    ttCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACGGTGAAGTTGCATACTATCGATATATCCACAATTTTAATATg              >  2:75620/1‑140 (MQ=255)                                                                                                                    ttCTCTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACGGTGAAGTTGCATACTATCGATATATCCACAATTTTAATATg              >  2:314482/1‑140 (MQ=255)                                                                                                                     tctcTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACGGTGAAGTTGCATACTATCGATATATCCACAATTTTAATATgg             <  1:817045/140‑1 (MQ=255)                                                                                                                     tctcTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACGGTGAAGTTGCATACTATCGATATATCCACAATTTTAATATgg             >  1:526771/1‑140 (MQ=255)                                                                                                                     tctcTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACGGTGAAGTTGCATACTATCGATATATCCACAATTTTAATATgg             <  2:293646/140‑1 (MQ=255)                                                                                                                     tctcTGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACGGTGAAGTTGCATACTATCGATATATCCACAATTTTAATATgg             >  1:201746/1‑140 (MQ=255)                                                                                                                      ctctGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACGGTGAAGTTGCATACTATCGATATATCCACAATTTTAATATGGc            >  2:597312/1‑140 (MQ=255)                                                                                                                      ctctGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACGGTGAAGTTGCATACTATCGATATATCCACAATTTTAATATGGc            >  2:157983/1‑140 (MQ=255)                                                                                                                       tctGATGAGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACGGTGAAGTTGCATACTATCGATATATCCACAATTTTAATATGGcc           <  2:1011768/140‑1 (MQ=255)                                                                                                                         tgatgaGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACGGTGAAGTTGCATACTATCGATATATCCACAATTTTAATATGGCCtt         >  2:151217/1‑140 (MQ=255)                                                                                                                         tgatgaGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACGGTGAAGTTGCATACTATCGATATATCCACAATTTTAATATGGCCtt         >  1:183779/1‑140 (MQ=255)                                                                                                                              aGGACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACGGTGAAGTTGCATACTATCGATATATCCACAATTTTAATATGGCCTTGTTTa    >  1:967566/1‑140 (MQ=255)                                                                                                                                gACCG‑TTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACGGTGAAGTTGCATACTATCGATATATCCACAATTTTAATATGGCCTTGTTTAAt  >  1:387415/1‑140 (MQ=255)                                                                                                                                     |                                                                                                                                        ATGCCGATGCGACTGAAAAAGTAATTTCGGTGCAGGAAATGCACGCACAGATCAAATAATAGCGTGTCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCGTTTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACGGTGAAGTTGCATACTATCGATATATCCACAATTTTAATATGGCCTTGTTTAAT  >  NC_000913/33980‑34247

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑