Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	868,422	(C)8→7	coding (903/1872 nt)	gsiA →	glutathione ABC transporter ATP binding subunit GsiA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	868,415	0	C	.	100.0%	232.5 / NA	54	coding (896/1872 nt)	gsiA	glutathione ABC transporter ATP binding subunit GsiA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (27/27);  total (27/27)

AACAGATTTTTCATGCACCGCAACATCCTTACACCCGTGCGCTGTTAGCTGCTGTTCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGGCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTGCGCAGCGGTTTGTTGAATCGCGTAACGCGGGAAGTGCATGCCGTTGAGAAAGTC  >  NC_000913/868280‑868548                                                                                                                                        |                                                                                                                                      aaCAGATTTTTCATGCACCGCAACATCCTTACACCCGTGCGCTGTTAGCTGCTGTTCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGGccccc                                                                                                                                   <  2:358560/140‑1 (MQ=255)        ttttCATGCACCGCAACATCCTTACACCCGTGCGCTGTTAGCTGCTGTTCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGa                                                                                                                           <  2:988323/140‑1 (MQ=255)        ttttCATGCACCGCAACATCCTTACACCCGTGCGCTGTTAGCTGCTGTTCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGa                                                                                                                           <  1:526415/140‑1 (MQ=255)         tttCATGCACCGCAACATCCTTACACCCGTGCGCTGTTAGCTGCTGTTCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAg                                                                                                                          <  2:643737/140‑1 (MQ=255)                cACCGCAACATCCTTACACCCGTGCGCTGTTAGCTGCTGTTCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGaaaa                                                                                                                   <  1:31695/140‑1 (MQ=255)                cACCGCAACATCCTTACACCCGTGCGCTGTTAGCTGCTGTTCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGaaaa                                                                                                                   <  2:938855/140‑1 (MQ=255)                  ccGCAACATCCTTACACCCGTGCGCTGTTAGCTGCTGTTCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACg                                                                                                                 <  1:250415/140‑1 (MQ=255)                     cAACATCCTTACACCCGTGCGCTGTTAGCTGCTGTTCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACggtg                                                                                                              >  2:765780/1‑140 (MQ=255)                             ttACACCCGTGCGCTGTTAGCTGCTGTTCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATgg                                                                                                      >  1:928915/1‑140 (MQ=255)                                  cccGTGCGCTGTTAGCTGCTGTTCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCTGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAAc                                                                                                 >  2:860239/1‑140 (MQ=255)                                  cccGTGCGCTGTTAGCTGCTGTTCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAAc                                                                                                 >  1:116884/1‑140 (MQ=255)                                   ccGTGCGCTGTTAGCTGCTGTTCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAAcc                                                                                                <  2:918166/140‑1 (MQ=255)                                   ccGTGCGCTGTTAGCTGCTGTTCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAAcc                                                                                                <  1:765780/140‑1 (MQ=255)                                     gTGCGCTGTTAGCTGCTGTTCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTg                                                                                              >  1:118826/1‑140 (MQ=255)                                      tGCGCTGTTAGCTGCTGTTCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGt                                                                                             >  1:231334/1‑140 (MQ=255)                                      tGCGCTGTTAGCTGCTGTTCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGt                                                                                             >  1:223639/1‑140 (MQ=255)                                      tGCGCTGTTAGCTGCTGTTCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGt                                                                                             >  2:1033699/1‑140 (MQ=255)                                      tGCGCTGTTAGCTGCTGTTCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGt                                                                                             >  1:824359/1‑140 (MQ=255)                                           tGTTAGCTGCTGTTCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTAc                                                                                        <  2:118826/140‑1 (MQ=255)                                            gTTAGCTGCTGTTCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACg                                                                                       <  2:42887/140‑1 (MQ=255)                                             ttAGCTGCTGTTCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGa                                                                                      <  2:1043927/140‑1 (MQ=255)                                             ttAGCTGCTGTTCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGa                                                                                      <  2:733645/140‑1 (MQ=255)                                               aGCTGCTGTTCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGt                                                                                    <  2:612629/140‑1 (MQ=255)                                                    ctgTTCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTa                                                                               <  2:605386/140‑1 (MQ=255)                                                            cAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTc                                                                       <  2:928915/140‑1 (MQ=255)                                                              aCTTGGTGCGATGAAAGGGTTAGATTATCCCTGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCAc                                                                     <  1:860239/140‑1 (MQ=255)                                                               cTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTc                                                                       >  1:311262/1‑137 (MQ=255)                                                               cTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTc                                                                       <  2:311262/137‑1 (MQ=255)                                                                         tGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCtt                                                          >  1:358008/1‑140 (MQ=255)                                                                               ggTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAACACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTGCGCa                                                    <  2:824359/140‑1 (MQ=255)                                                                                  tAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTGCGCAGCg                                                 <  1:431204/140‑1 (MQ=255)                                                                                         tCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCGGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTGCGCAGCGGTttgtt                                          >  2:1073392/1‑140 (MQ=255)                                                                                          ccccGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTGCGCAGCGGTttgttg                                         >  2:843045/1‑140 (MQ=255)                                                                                              gACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAGCGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTGCGCAGCGGTTTGTTGAATc                                     >  2:1074830/1‑140 (MQ=255)                                                                                              gACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTGCGCAGCGGTTTGTTGAATc                                     >  1:670882/1‑140 (MQ=255)                                                                                              gACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTGCGCAGCGGTTTGTTGAATc                                     >  1:347792/1‑140 (MQ=255)                                                                                              gACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTGCGCAGCGGTTTGTTGAATc                                     >  1:354071/1‑140 (MQ=255)                                                                                              gACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTGCGCAGCGGTTTGTTGAATc                                     >  1:82099/1‑140 (MQ=255)                                                                                                cGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTGCGCAGCGGTTTGTTGAATcgc                                   <  1:1074830/140‑1 (MQ=255)                                                                                                     cccGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTGCGCAGCGGTTTGTTGAATCGCGTAAc                              <  2:478450/140‑1 (MQ=255)                                                                                                          tGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTGCGCAGCGGTTTGTTGAATCGCGTAACGCggg                         <  1:1033699/140‑1 (MQ=255)                                                                                                              atCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTGCGCAGCGGTTTGTTGAATCGCGTAACGCGGGAAGt                     >  1:956724/1‑140 (MQ=255)                                                                                                                cGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTGCGCAGCGGTTTGTTGAATCGCGTAACGCGGGAAGTGc                   <  2:347792/140‑1 (MQ=255)                                                                                                                 gCTTGAACATCCAGCGAAACAGG‑CCCTCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTGCGCAGCGGTTTGTTGAATCGCGTAACGCGGGAAGTGCa                  <  1:843045/140‑1 (MQ=255)                                                                                                                 gCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAAGCTTGTCACCCGTTTCCCTTTGCGCAGCGGTTTGTTGAATCGCGTAACGCGGGAAGTGCa                  <  2:223639/140‑1 (MQ=255)                                                                                                                  cTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTGCGCAGCGGTTTGTTGAATCGCGt                                 >  2:472648/1‑124 (MQ=255)                                                                                                                  cTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTGCGCAGCGGTTTGTTGAATCGCGt                                 <  1:472648/124‑1 (MQ=255)                                                                                                                  cTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTGCGCAGCGGTTTGTTGAATCGCGTAACGCGGGAAGTGCAt                 >  1:361051/1‑140 (MQ=255)                                                                                                                   ttGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTGCGCCGCGGTTTGTTGAATCGCGTAATGCGGGTAGTGCATg                >  2:766384/1‑140 (MQ=255)                                                                                                                    tGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTGCGCAGCGGTTTGTTGAATCGCGTAACGCGGGAAGTGCa                  <  2:449877/137‑1 (MQ=255)                                                                                                                    tGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTGCGCAGCGGTTTGTTGAATCGCGTAACGCGGGAAGTGCa                  >  1:449877/1‑137 (MQ=255)                                                                                                                      aaCATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTGCGCAGCGGTTTGTTGAATCGCGTAACGCGGGAAGTGCATGCCg             >  2:45840/1‑140 (MQ=255)                                                                                                                      aaCATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTGCGCAGCGGTTTGTTGAATCGCGTAACGCGGGAAGTGCATGCCg             >  2:288843/1‑140 (MQ=255)                                                                                                                                gAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTGCGCAGCGGTTTGTTGAATCGCGTAACGCGGGAAGTGCATGCCGTTGAGAAAGt   >  1:522006/1‑140 (MQ=255)                                                                                                                                 aaaCAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTGCGCAGCGGTTTGTTGAATCGCGTAACGCGGGAAGTGCATGCCGTTGAGAAAGTc  >  2:766519/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                      AACAGATTTTTCATGCACCGCAACATCCTTACACCCGTGCGCTGTTAGCTGCTGTTCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGGCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTGCGCAGCGGTTTGTTGAATCGCGTAACGCGGGAAGTGCATGCCGTTGAGAAAGTC  >  NC_000913/868280‑868548

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑