| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,182,826 | (T)6→5 | coding (4/1047 nt) coding (795/795 nt) |
potD ← potC ← |
spermidine preferential ABC transporter periplasmic binding protein spermidine preferential ABC transporter membrane subunit PotC |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,182,821 | 0 | T | . | 100.0% | 237.7 / NA | 55 | coding (9/1047 nt) | potD | spermidine preferential ABC transporter periplasmic binding protein |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base . (24/31); total (24/31) | |||||||||||
TCTTTGGTGAACTGTTCAAGCAGTCCTGGCGGCACGTACTCGGTCCAGTTGTAGAAATACAGCGTGTTGTTGTCATCGGCGTGAGCGGCGCTCATGCCCAGTGCCAGAGCACCCGCCGCGAGCAGGTGGCGTGACCATTTTTTCATTTAACGTCCCCTGTGTTACCTTTCGTTTTATCACGAGCAATAAGCTGGCTGGCAATAACCATCACCAGCGACAGCACCAGTAATATGGTTGCCAGCGCGTTAACTTCCGGCGATACGC > NC_000913/1182684‑1182947 | tCTTTGGTGAACTGTTCAAGCAGTCCTGGCGGCACGTACTCGGTCCAGTTGTAGAAATACAGCGTGTTGTTGTCATCGGCGTGAGCGGCGCTCATGCCCAGTGCCAGAGCACCCGCCGCGAGCAGGTGGCGTGACCAttt < 2:423998/140‑1 (MQ=255)tCTTTGGTGAACTGTTCAAGCAGTCCTGGCGGCACGTACTCGGTCCAGTTGTAGAAATACAGCGTGTTGTTGTCATCGGCGTGAGCGGCGCTCATGCCCAGTGCCAGAGCACCCGCCGCGAGCAGGTGGCGTGACCAttt > 2:1186843/1‑140 (MQ=255) cTTTGGTGAACTGTTCAAGCAGTCCTGGCGGCACGTACTCGGTCCAGTTGTAGAAATACAGCGTGTTGTTGTCATCGGCGTGAGCGGCGCTCATGCCCAGTGCCAGAGCACCCGCCGCGAGCAGGTGGCGTGACCAtttt < 2:27755/140‑1 (MQ=255) ggTGAACTGTTCAAGCAGTCCTGGCGGCACGTACTCGGTCCAGTTGTAGAAATACAGCGTGTTGTTGTCATCGGCGTGAGCGGCGCTCATGCCCAGTGCCAGAGCACCCGCCGCGAGCAGGTGGCGTGACCAtttttcat < 1:426254/140‑4 (MQ=255) aaCTGTCCAAGCAGTCCGGGGGGCACGTACTCGGTCCAGTTGATGAAATCCAGCGTGTTGTTGTCATCGGCGTGGGGGGCGCTCATGCCCAGTGCCAGAGCCCCCGCCGCGAGCAGGTGGCGTGACCA‑TTTTTCATTTaa < 2:1115156/140‑1 (MQ=255) ttCAAGCAGTCTTGGCGGCACGTACTCGGTCCAGTTGTAGAAATACAGCGTGTTGTTGTCATCGGCGTGAGCGGCGCTCATGCCCAGTGCCAGAGCCCCCGCCGCGAGCAGGTGGCGTGACCA‑TTTTTCATTTAACGTcc < 1:129917/140‑1 (MQ=255) ttCAAGCAGTCCTGGCGGCACGTACTCGGTCCAGTTGTAGAAATACAGCGTGTTGTTGTCATCGGCGTGAGCGGCGCTCATGCCCAGTGCCAGAGCACCCGCCGCGAGCAGGTGGCGTGACCA‑TTTTTCATTTAACGTcc > 1:413858/1‑140 (MQ=255) ttCAAGCAGTCCTGGCGGCACGTACTCGGTCCAGTTGTAGAAATACAGCGTGTTGTTGTCATCGGCGTGAGCGGCGCTCATGCCCAGTGCCAGAGCACCCGCCGCGAGCAGGTGGCGTGACCA‑TTTTTCATTTAACGTcc > 1:408381/1‑140 (MQ=255) ttCAAGCAGTCCTGGCGGCACGTACTCGGTCCAGTTGTAGAAATACAGCGTGTTGTTGTCATCGGCGTGAGCGGCGCTCATGCCCAGTGCCAGAGCACCCGCCGCGAGCAGGTGGCGTGACCA‑TTTTTCATTTAACGTcc < 1:499219/140‑1 (MQ=255) ttCAAGCAGTCCTGGCGGCACGTACTCGGTCCAGTTGTAGAAATACAGCGTGTTGTTGTCATCGGCGTGAGCGGCGCTCATGCCCAGTGCCAGAGCACCCGCCGCGAGCAGGTGGCGTGACCA‑TTTTTCATTTAACGTcc > 2:347922/1‑140 (MQ=255) tCAAGCAGTCCTGGCGGCACGTACTCGGTCCAGTTGTAGAAATACAGCGTGTTGTTGTCATCGGCGTGAGCGGTGCTCATGCCCAGTGCCAGAGCACCCGCCGCGAGCAGGTGGCGTGACCA‑TTTTTCATTTAACGTccc < 1:1140516/140‑1 (MQ=255) tCAAGCAGTCCTGGCGGCACGTACTCGGTCCAGTTGTAGAAATACAGCGTGTTGTTGTCATCGGCGTGAGCGGCGCTCATGCCCAGTGCCAGAGCACCCGCCGCGAGCAGGTGGCGTGACCA‑TTTTTCATTTAACGTccc < 2:413858/140‑1 (MQ=255) tCAAGCAGTCCTGGCGGCACGTACTCGGTCCAGTTGTAGAAATACAGCGTGTTGTTGTCATCGGCGTGAGCGGCGCTCATGCCCAGTGCCAGAGCACCCGCCGCGAGCAGGTGGCGTGACCA‑TTTTTCATTTAACGTccc > 2:539023/1‑140 (MQ=255) ccTGGCGGCACGTACTCGGTCCAGTTGTAGAAATACAGCGTGTTGTTGTCATCGGCGTGAGCGGCGCTCATGCCCAGTGCCAGAGCACCCGCCGCGAGCAGGTGGCGTGACCA‑TTTTTCATTTAACGTCCCCtgt < 2:325291/135‑1 (MQ=255) ccTGGCGGCACGTACTCGGTCCAGTTGTAGAAATACAGCGTGTTGTTGTCATCGGCGTGAGCGGCGCTCATGCCCAGTGCCAGAGCACCCGCCGCGAGCAGGTGGCGTGACCA‑TTTTTCATTTAACGTCCCCtgt > 1:325291/1‑135 (MQ=255) ccTGGCGGCACGTACTCGGTCCAGTTGTAGAAATACAGCGTGTTGTTGTCATCGGCGTGAGCGGCGCTCATGCCCAGTGCCAGAGCACCCGCCGCGAGCAGGTGGCGTGACCA‑TTTTTCATTTAACGTCCCCTGTGTTAc > 1:671263/1‑140 (MQ=255) ccTGGCGGCACGTACTCGGTCCAGTTGTAGAAATACAGCGTGTTGTTGTCATCGGCGTGAGCGGCGCTCATGCCCAGTGCCAGAGCACCCGCCGCGAGCAGGTGGCGTGACCA‑TTTTTCATTTAACGTCCCCTGTGTTAc > 1:320441/1‑140 (MQ=255) ccTGGCGGCACGTACTCGGTCCAGTTGTAGAAATACAGCGTGTTGTTGTCATCGGCGTGAGCGGCGCTCATGCCCAGTGCCAGAGCACCCGCCGCGAGCAGGTGGCGTGACCA‑TTTTTCATTTAACGTCCCCTGTGTTAc > 2:1008288/1‑140 (MQ=255) ccTGGCGGCACGTACTCGGTCCAGTTGTAGAAATACAGCGTGTTGTTGTCATCGGCGTGAGCGGCGCTCATGCCCAGTGCCAGAGCACCCGCCGCGAGCAGGTGGCGTGACCA‑TTTTGCATTTAACGTCCCCTGTGTTAc > 1:851229/1‑140 (MQ=255) tGGCGGCACGTACTCGGTCCAGTTGTAGAAATACAGCGTGTTGTTGTCATCGGCGTGAGCGGCGCTCATGCCCAGTGCCAGAGCACCCGCCGCGAGCAGGTGGCGTGACCA‑TTTTTCATTTAACGTCCCCTGTGTTACCt < 1:1008288/140‑1 (MQ=255) aCGTACTCGGTCCAGTTGTAGAAATACAGCGTGTTGTTGTCATCGGCGTGAGCGGCGCTCATGCCCAGTGCCAGAGCACCCGCCGCGAGCAGGTGGCGTGACCA‑TTTTTCATTTAACGTCCCCTGTGTTACCTTTCGttt > 2:384069/1‑140 (MQ=255) tCGGTCCAGTTGTAGAAATACAGCGTGTTGTTGTCATCGGCGTGAGCGGCGCTCATGCCCAGTGCCAGAGCACCCGCCGCGAGCAGGTGGCGTGACCA‑TTTTTCATTTAACGTCCCCTGTGTTACCTTTCGTTTTATCAc < 1:347922/140‑1 (MQ=255) tCGGTCCAGTTGTAGAAATACAGCGTGTTGTTGTCATCGGCGTGAGCGGCGCTCATGCCCAGTGCCAGAGCACCCGCCGCGAGCAGGTGGCGTGACCA‑TTTTTCATTTAACGTCCCCTGTGTTACCTTTCGTTTTATCAc < 1:384069/140‑1 (MQ=255) gTCCAGTTGTAGAAATACAGCGTGTTGTTGTCATCGGCGTGAGCGGCGCTCATGCCCAGTGCCAGAGCACCCGCCGCGAGCAGGTGGCGTGACCA‑TTTTTCATTTAACGTCCCCTGTGTTACCTTTCGTTTTATCACGAg < 1:539023/140‑1 (MQ=255) ccgTTGTAGAAATACAGCGTGTTGTTGTCATCGGCGTGAGCGGCGCTCATGCCCAGTGCCAGAGCACCCGCCGCGAGCAGGTGGCGTGACCA‑TTTTTCATTTAACGTCCCCTGTGTTACCTTTCGTTTTATCACGAGCaa > 2:150350/3‑140 (MQ=255) gTTGTAGAAATACAGCGTGTTGTTGTCATCGGCGTGAGCGGCGCTCATGCCCAGTGCCAGAGCACCCGCCGCGAGCAGGTGGCGTGACCA‑TTTTTCATTTAACGTCCCCTGTGTTACCTTTCGTTTTATCACGAGCAATa < 2:821385/140‑1 (MQ=255) gTTGTAGAAATACAGCGTGTTGTTGTCATCGGCGTGAGCGGCGCTCATGCCCAGTGCCAGAGCACCCGCCGCGAGCAGGTGGCGTGACCA‑TTTTTCATTTAACGTCCCCTGTGTTACCTTTCGTTTTATCACGAGCAATa > 2:742985/1‑140 (MQ=255) tAGAAATACAGCGTGTTGTTGTCATCGGCGTGAGCGGCGCTCATGCCCAGTGCCAGAGCACCCGCCGCGAGCAGGTGGCGTGACCA‑TTTTTCATTTAACGTCCCCTGTGTTACCTTTCGTTTTATCACGAGCAATAAgct > 1:935111/1‑140 (MQ=255) gAAATACAGCGTGTTGTTGTCATCGGCGTGAGCGGCGCTCATGCCCGGTGCCAGAGCACCCGCCGCGAGCAGGTGGCGTGACCA‑TTTTTCATTTAACGTCCCCTGTGTTACCTTTCGTTTTATCACGAGCAATAAgctgg < 1:1186843/140‑1 (MQ=255) gAAATACAGCGTGTTGTTGTCATCGGCGTGAGCGGCGCTCATGCCCAGTGCCAGAGCACCCGCCGCGAGCAGGTGGCGTGACCA‑TTTTTCATTTAACGTCCCCTGTGTTACCTTTCGTTTTATCAc > 2:616581/1‑126 (MQ=255) gAAATACAGCGTGTTGTTGTCATCGGCGTGAGCGGCGCTCATGCCCAGTGCCAGAGCACCCGCCGCGAGCAGGTGGCGTGACCA‑TTTTTCATTTAACGTCCCCTGTGTTACCTTTCGTTTTATCAc < 1:616581/126‑1 (MQ=255) gAAATACAGCGTGTTGTTGTCATCGGCGTGAGCGGCGCTCATGCCCAGTGCCAGAGCACCCGCCGCGAGCAGGTGGCGTGACCA‑TTTTTCATTTAACGTCCCCTGTGTTACCTTTCGTTTTATCACGAGCAATAAgctgg > 1:1088791/1‑140 (MQ=255) aaaTACGGCGTTTTGTTGTCATCGGCGTGAGCGGCGCTCATGCCCAGTGCCAGAGCCCCCGCCGCGAGCAGGTGGCGTGACCA‑TTTTTCATTTAACGTCCCCTGTGTTACCTTTCGTTTTATCACGAGCAATAAgctggc < 2:90802/140‑1 (MQ=255) aaaTACAGCGTTTTTTTGTCATCGGCGTGAGCGGCTCTCATGCCCAGTGCCAGAGCACCCGCCGCGAGCAGGTGGCGTGACCA‑TTTTTCATTTAACGTCCCCTGTGTTACCTTTCGTTTTATCACGAGCAATAAgctggc < 2:1088791/140‑1 (MQ=255) aaTACAGCGTGTTGTTGTCATCGGCGTGAGCGGCGCTCATGCCCAGTGCCAGAGCACCCGCCGCGAGCAGGTGGCGTGACCA‑TTTTTCATTTAACGTCCCCTGTGTTACCTTTCGTTTTATCACGAGCAATAAgctggct < 1:704488/140‑1 (MQ=255) aTACAGCGTGTTGTTGTCATCGGCGTGAGCGGCGCTCATGCCCAGTGCCAGAGCACCCCCCGCGAGCAGGGGGCGTGACCA‑TTTTTCATTTAACGTCCCCTGTGTTACCTTTCGTTTTATCACGAGCAATAAgctggctg > 1:417399/1‑140 (MQ=255) aCAGCGTGTTGTTGTCATCGGCGTGAGCGGCGCTCATGCCCAGTGCCAGAGCACCCGCCGCGAGCAGGTGGCGTGACCA‑TTTTTCATTTAACGTCCCCTGTGTTACCTTTCGTTTTATCACGAGCAATAAGCtggctggc > 2:1038768/1‑140 (MQ=255) aCAGCGTGTTGTTGTCATCGGCGTGAGCGGCGCTCATGCCCAGTGCCAGAGCACCCGCCGCGAGCAGGTGGCGTGACCA‑TTTTTCATTTAACGTCCCCTGTGTTACCTTTCGTTTTATCACGAGCAATAAGCtggctggc > 2:195860/1‑140 (MQ=255) cAGCGTGTTGTTGTCATCGGCGTGAGCGGCGCTCATGCCCAGTGCCAGAGCTCCCGCCGCGAGCAGGTGGCGTGACCA‑TTTTTCATTTAACGTCCCCTGTGTTACCTTTCGTTTTATCACGAGCAATAAGCTGGCTGGCa < 1:150350/140‑1 (MQ=255) cAGCGTGTTGTTGTCATCGGCGTGAGCGGCGCTCATGCCCAGTGCCAGAGCACCCGCCGCGAGCAGGTGGCGTGACCA‑TTTTTCATTTAACGTCCCCTGTGTTACCTTTCGTTTTATCACGAGCAATAAGCTGGCTGGCa < 1:308613/140‑1 (MQ=255) agggttttgttgtCATCGGGGTGAGCGGCGCTCATGCCCAGTGCAAGACCCCCCGCCGCGAGCAGGTGGCGTGACCA‑TTTTTCATTTAACGTCCCCTGTGTTACCTTTCGTTTTATCACGAGCAATAAGCTGGCTGGCaa < 2:417399/134‑1 (MQ=255) tgttgttgtCATCGGCGTGAGCGGCGCTCATGCCCAGTGCCAGAGCCCCCGCCGCGAGCAGGTGGCGTGACCA‑TTTTTCATTTAACGTCCCCTGTGTTACCTTTCGTTTTATCACGAGCAATAAGCTGGCTGGCAATAAc < 1:1080445/140‑1 (MQ=255) tgttgttgtCATCGGCGTGAGCGGCGCTCATGCCCAGTGCCAGAGCACCCGCCGCGAGCAGGTGGCGTGACCA‑TTTTTCATTTAACGTCCCCTGTGTTACCTTTCGTTTTATCACGAGCAATAAGCTGGCTGGCAATAAc > 2:334075/1‑140 (MQ=255) tgttgtCATCGGCGTGAGCGGCGCTCATGCCCAGTGCCAGAGCACCCGCCGCGAGCAGGTGGCGTGACCA‑TTTTTCATTTAACGTCCCCTGTGTTACCTTTCGTTTTATCACGAGCAATAAGCTGGCTGGCAATAACCAt > 2:661749/1‑140 (MQ=255) ttgtCATCGGCGTGAGCGGCGCTCATGCCCAGTGCCAGAGCACCCGCCGCGAGCAGGTGGCGTGACCA‑TTTTTCATTTAACGTCCCCTGTGTTACCTTTCGTTTTATCACGAGCAATAAGCTGGCTGGCAATAACCATCa < 1:56677/140‑1 (MQ=255) tCATCGGCGTGAGCGGCGCTCATGCCCAGTGCCAGAGCACCCGCCGCGAGCAGGTGGCGTGACCA‑TTTTTCATTTAACGTCCCCTGTGTTACCTTTCGTTTTATCACGAGCAATAAGCTGGCTGGCAATAACCATCACCa > 2:62031/1‑140 (MQ=255) cATCGGCGTGAGCGGCGCTCATGCCCAGTGCCAGAGCACCCGCCGCGAGCAGGTGGCGTGACCA‑TTTTTCATTTAACGTCCCCTGTGTTACCTTTCGTTTTATCACGAGCAATAAGCTGGCTGGCAATAACCATCACCAg < 2:935111/140‑1 (MQ=255) tCGGCGTGAGCGGCGCTCATGCCCAGTGCCAGAGCACCCGCCGCGAGCAGGTGGCGTGACCA‑TTTTTCATTTAACGTCCCCTGTGTTACCTTTCGTTTTATCACGAGCAATAAGCTGGCTGGCAATAACCATCACCAGCg < 1:1038768/140‑1 (MQ=255) cGGCGTGAGCGGCGCTCATGCCCAGTGCCAGAGCCCCCGCCGCGAGCAGGTGGCGTGACCA‑TTTTTCATTTAACGTCCCCTGTGTTACCTTTCGTTTTATCACGAGCAATAAGCTGGCTGGCAATAACCATCACCAGCGa < 1:628967/140‑1 (MQ=255) cGGCGTGAGCGGCGCTCATGCCCAGTGCCAGAGCACCCGCCGCGAGCAGGTGGCGTGACCA‑TTTTTCATTTAACGTCCCCTGTGTTACCTTTCGTTTTATCACGAGCAATAAGCTGGCTGGCAATaa < 2:51296/127‑1 (MQ=255) cGGCGTGAGCGGCGCTCATGCCCAGTGCCAGAGCACCCGCCGCGAGCAGGTGGCGTGACCA‑TTTTTCATTTAACGTCCCCTGTGTTACCTTTCGTTTTATCACGAGCAATAAGCTGGCTGGCAATaa > 1:51296/1‑127 (MQ=255) cGTGAGCGGCGCTCATGCCCAGTGCCAGAGCACCCGCCGCGAGCAGGTGGCGTGACCA‑TTTTTCATTTAACGTCCCCTGTGTTACCTTTCGTTTTATCACGAGCAATAAGCTGGCTGGCAATAACCATCACCAGCGACAg > 1:16068/1‑140 (MQ=255) cGTGAGCGGCGCTCATGCCCAGTGCAAGACCACCCGCCGCGAGCAGGTGGCGTGACCA‑TTTTTCATTTAACGTCCCCTGTGTTACCTTTCGTTTTATCACGAGCAATAAGCTGGCTGGCAATAACCATCACCAGCGACAg < 1:334075/140‑1 (MQ=255) gAGCGGCGCTCATGCCCAGTGCCAGAGCACCCGCCGCGAGCAGGTGGCGTGACCA‑TTTTTCATTTAACGTCCCCTGTGTTACCTTTCGTTTTATCACGAGCAATAAGCTGGCTGGCAATAACCATCACCAGCGACAGCAc < 2:336401/140‑1 (MQ=255) gAGCGGCGCTCATGCCCAGTGCCAGAGCACCCGCCGCGAGCAGGTGGCGTGACCA‑TTTTTCATTTAACGTCCCCTGTGTTACCTTTCGTTTTATCACGAGCAATAAGCTGGCTGGCAATAACCATCACCAGCGACAGCAc < 1:878232/140‑1 (MQ=255) gAGCGGCGCTCATGCCCAGTGCCAGAGCACCCGCCGCGAGCAGGTGGCGTGACCA‑TTTTTCATTTAACGTCCCCTGTGTTACCTTTCGTTTTATCACGAGCAATAAGCTGGCTGGCAATAACCATCACCAGCGACAGCAc < 1:742985/140‑1 (MQ=255) agagCACCCGCCGCGAGCAGGTGGCGTGACCA‑TTTTTCATTTAACGTCCCCTGTGTTACCTTTCGTTTTATCACGAGCAATAAGCTGGCTGGCAATAACCATCACCAGCGACAGCACCAGTAATATGGTTGCCAGCGCGt > 1:997025/1‑140 (MQ=255) cgAGCAGGTGGCGTGACCA‑TTTTTCATTTAACGTCCCCTGTGTTACCTTTCGTTTTATCACGAGCAATAAGCTGGCTGGCAATAACCATCACCAGCGACAGCACCAGTAATATGGTTGCCAGCGCGTTAACTTCCGGCGa < 2:210579/140‑1 (MQ=255) aGGTGGCGTGACCA‑TTTTTCATTTAACGTCCCCTGTGTTACCTTTCGTTTTATCACGAGCAATAAGCTGGCTGGCAATAACCATCACCAGCGACAGCACCAGTAATATGGTTGCCAGCGCGTTAACTTCCGGCGATACGc < 1:661749/140‑1 (MQ=255) | TCTTTGGTGAACTGTTCAAGCAGTCCTGGCGGCACGTACTCGGTCCAGTTGTAGAAATACAGCGTGTTGTTGTCATCGGCGTGAGCGGCGCTCATGCCCAGTGCCAGAGCACCCGCCGCGAGCAGGTGGCGTGACCATTTTTTCATTTAACGTCCCCTGTGTTACCTTTCGTTTTATCACGAGCAATAAGCTGGCTGGCAATAACCATCACCAGCGACAGCACCAGTAATATGGTTGCCAGCGCGTTAACTTCCGGCGATACGC > NC_000913/1182684‑1182947 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |