Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,265,990 (G)6→7 coding (209/1080 nt) alr → alanine racemase 1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,265,9841.G97.4% 136.4 / ‑2.9 38coding (203/1080 nt)alralanine racemase 1
Reads supporting (aligned to +/- strand):  ref base . (0/1);  new base G (16/21);  total (16/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.55e-01

GAACTGGCCCCTGCCAGTAAAATGGTTGCGGTGGTGAAAGCGAACGCTTATGGTCACGGTCTTCTTGAGACCGCGCGAACGCTCCCCGATGCTGACGCCTTTGGCGTAGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGC‑GGGGGG‑AATCACCAAACCTGTACTGTTACTCGAAGGCTTTTTTGATGCCAGAGATCTGCCGACGATTTCTGCGCAACATTTTCATACCGCCGTGCATAACGAAGAACAGCTGGCTGCGCTGGAAGAGGCTAGCC  >  NC_000913/4265845‑4266118
                                                                                                                                            |                                                                                                                                       
gagcTGGCCCCTGCCAGTAAAATGGTTGCGGTGGTGAAAGCGAACGCTTATGGTCACGGTCTTCTTGAGACCGCGCGAACGCTCCCCGATGCTGACGCCTTTGGCGTAGCCCGTCTCGAAGAAGCTCTGCGACtgcgtgc                                                                                                                                          <  1:580305/137‑1 (MQ=255)
      gCCCCTGCCAGTAAAATGGTTGCGGTGGTGAAAGCGAACGCTTATGGTCACGGTCTTCTTGAGACCGCGCGAACGCTCCCCGATGCTGACGCCTTTGGCGTAGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGC‑gggggg                                                                                                                                   >  2:678478/1‑140 (MQ=255)
      gCCCCTGCCAGTAAAATGGTTGCGGTGGTGAAAGCGAACGCTTATGGTCACGGTCTTCTTGAGACCGCGCGAACGCTCCCCGATGCTGACGCCTTTGGCGTAGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGC‑gggggg                                                                                                                                   >  1:153290/1‑140 (MQ=255)
        cccTGCCAGTAAAATGGTTGCGGTGGTGAAAGCGAACGCTTATGGTCACGGTCTTCTTGAGACCGCGCGAACGCTCCCCGATGCTGACGCCTTTGGCGTAGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGC‑ggggggga                                                                                                                                 <  2:10996/140‑3 (MQ=255)
        cccTGCCAGTAAAATGGTTGCGGTGGTGAAAGCGAACGCTTATGGTCACGGTCTTCTTGAGACCGCGCGAACGCTCCCCGATGCTGACGCCTTTGGCGTAGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGC‑ggggggga                                                                                                                                 <  2:153290/140‑3 (MQ=255)
        cccTGCCAGTAAAATGGTTGCGGTGGTGAAAGCGAACGCTTATGGTCACGGTCTTCTTGAGACCGCGCGAACGCTCCCCGATGCTGACGCCTTTGGCGTAGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGC‑ggggggga                                                                                                                                 <  2:256802/140‑3 (MQ=255)
        cccTGCCAGTAAAATGGTTGCGGTGGTGAAAGCGAACGCTTATGGTCACGGTCTTCTTGAGACCGCGCGAACGCTCCCCGATGCTGACGCCTTTGGCGTAGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGC‑ggggggga                                                                                                                                 <  1:38970/140‑3 (MQ=255)
         ccTGCCAGTAAAATGGTTGCGGTGGTGAAAGCGAACGCTTATGGTCACGGTCTTCTTGAGACCGCGCGAACGCTCCCCGATGCTGACGCCTTTGGCGTAGCCCGTCTCGAAGAAGCTCTGCGACtgcgtgc                                                                                                                                          <  1:661161/131‑1 (MQ=255)
         ccTGCCAGTAAAATGGTTGCGGTGGTGAAAGCGAACGCTTATGGTCACGGTCTTCTTGAGACCGCGCGAACGCTCCCCGATGCTGACGCCTTTGGCGTAGCCCGTCTCGAAGAAGCTCTGCGACtgcgtgc                                                                                                                                          >  2:661161/1‑131 (MQ=255)
            gcaaGTAAAAT‑GTTGCCGGGGTTAAAACGAACGCTTAAGGGCACGGTTTTCTTTAGGCCGGGGCAACCCCCCCCGATGCTGACGCCTTTGGCGTAGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGCGGGGGGG‑AATCAc                                                                                                                            <  2:144434/137‑1 (MQ=255)
            gCCAGTAAAATGGTTGCGGTGGTGAAAGCGAACGCTTATGGTCACGGTCTTCTTGAGACCGCGCGAACGCTCCCCGATGCTGACGCCTTTGGCGTAGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGCGGGGGGG‑AATCa                                                                                                                             >  1:1172571/1‑140 (MQ=255)
             ccAGTAAAATGGTTGCGGTGGTGAAAGCGAACGCTTATGGTCACGGTCTTCTTGAGACCGCGCGAACGCTCCCCGATGCTGACGCCTTTGGCGTAGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGCGGGGGGG‑AATCAc                                                                                                                            <  1:233006/140‑1 (MQ=255)
             ccAGTAAAATGGTTGCGGTGGTGAAAGCGAACGCTTATGGTCACGGTCTTCTTGAGACCGCGCGAACGCTCCCCGATGCTGACGCCTTTGGCGTAGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGCGGGGGGG‑AATCAc                                                                                                                            <  2:496907/140‑1 (MQ=255)
               aGTAAAATGGTTGCGGTGGTGAAAGCGAACGCTTATGGTCACGGTCTTCTTGAGACCGCGCGAACGCTCCCCGATGCTGACGCCTTTGGCGTAGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGCGGGGGGG‑AATCACCa                                                                                                                          <  1:26946/140‑1 (MQ=255)
                 tAAAATGGTTGCGGTGGTGAAAGCGAACGCTTATGGTCACGGTCTTCTTGAGACCGCGCGAACGCTCCCCGATGCTGACGCCTTTGGCGTAGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGCGGGGGGG‑AATCACCaaa                                                                                                                        >  2:456869/1‑140 (MQ=255)
                    aaTGGTTGCGGTGGTGAAAGCGAACGCTTATGGTCACGGTCTTCTTGAGACCGCGCGAACGCTCCCCGATGCTGACGCCTTTGGCGTAGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGCGGGGGGG‑AATCACCAAACCt                                                                                                                     <  2:1172571/140‑1 (MQ=255)
                     aTGGTTGCGGTGGTGAAAGCGAACGCTTATGGTCACGGTCTTCTTGAGACCGCGCGAACGCTCCCCGATGCTGACGCCTTTGGCGTAGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGCGGGGGGG‑AATCACCAAACCTg                                                                                                                    <  1:678478/140‑1 (MQ=255)
                     aTGGTTGCGGTGGTGAAAGCGAACGCTTATGGTCACGGTCTTCTTGAGACCGCGCGAACGCTCCCCGATGCTGACGCCTTTGGCGTAGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGCGGGGGGG‑AATCACCAAACCTg                                                                                                                    <  1:1038881/140‑1 (MQ=255)
                       ggTTGCGGTGGTGAAAGCGAACGCTTATGGTCACGGTCTTCTTGAGACCGCGCGAACGCTCCCCGATGCTGACGCCTTTGGCGTAGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGCGGGGGGG‑AATCACCAAACCTGTa                                                                                                                  <  2:407496/140‑1 (MQ=255)
                       ggTTGCGGTGGTGAAAGCGAACGCTTATGGTCACGGTCTTCTTGAGACCGCGCGAACGCTCCCCGATGCTGACGCCTTTGGCGTAGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGCGGGGGGG‑AATCACCAAACCTGTa                                                                                                                  <  2:875946/140‑1 (MQ=255)
                       ggTTGCGGTGGTGAAAGCGAACGCTTATGGTCACGGTCTTCTTGAGACCGCGCGAACGCTCCCCGATGCTGACGCCTTTGGCGTAGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGC‑GGGGGGAAATCACCAAACCTGTa                                                                                                                  <  1:63689/140‑1 (MQ=255)
                        gTTGCGGTGGTGAAAGCGAACGCTTATGGTCACGGTCTTCTTGAGACCGCGCGAACGCTCCCCGATGCTGACGCCTTTGGCGTAGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGCGGGGGGG‑AATCACCAAACCTGTAc                                                                                                                 >  2:763525/1‑140 (MQ=255)
                         ttGCGGTGGTGAAAGCGAACGCTTATGGTCACGGTCTTCTTGAGACCGCGCGAACGCTCCCCGATGCTGACGCCTTTGGCGTAGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGCGGGGGGG‑AATCAc                                                                                                                            >  1:163630/1‑128 (MQ=255)
                         ttGCGGTGGTGAAAGCGAACGCTTATGGTCACGGTCTTCTTGAGACCGCGCGAACGCTCCCCGATGCTGACGCCTTTGGCGTAGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGCGGGGGGG‑AATCAc                                                                                                                            <  2:163630/128‑1 (MQ=255)
                           gCGGTGGTGAAAGCGAACGCTTATGGTCACGGTCTTCTTGAGACCGCGCGAACGCTCCCCGATGCTGACGCCTTTGGCGTAGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGCGGGGGGG‑AATCACCa                                                                                                                          <  1:1063549/128‑1 (MQ=255)
                           gCGGTGGTGAAAGCGAACGCTTATGGTCACGGTCTTCTTGAGACCGCGCGAACGCTCCCCGATGCTGACGCCTTTGGCGTAGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGCGGGGGGG‑AATCACCa                                                                                                                          >  2:1063549/1‑128 (MQ=255)
                           gCGGTGGTGAAAGCGAACGCTTATGGTCACGGTCTTCTTGAGACCGCGCGAACGCTCCCCGATGCTGACGCCTTTGGCGTAGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGCGGGGGGG‑AATCACCAAACCTGTACTGt                                                                                                              >  2:523756/1‑140 (MQ=255)
                                                           tcttctTGAGACCGCGCGAACGCTCCCCGATGCTGACGCCTTTGGCGTAGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGCGGGGGGG‑AATCACCAAACCTGTACTGTTACTCGAAGGCTTTTTTGATGCCAGAGATCTg                                                                              <  1:335230/140‑1 (MQ=255)
                                                             ttcttGAGACCGCGCGAACGCTCCCCGATGCTGACGCCTTTGGCGTAGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGCGGGGGGG‑AATCACCAAACCTGTACTGTTACTCGAAGGCTTTTTTGATGCCAGAGATCTGcc                                                                            >  1:782062/1‑140 (MQ=255)
                                                                       cgcgcgAACGCTCCCCGATGCTGACGCCTTTGGCGTAGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGCGGGGGGG‑AATCACCAAACCTGTACTGTTACTCGAAGGCTTTTTTGATGCCAGAGATCTGCCGACGATTTCt                                                                  >  1:1005026/1‑140 (MQ=255)
                                                                         cgcgAACGCTCCCCGATGCTGACGCCTTTGGCGTAGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGCGGGGGGG‑AATCACCAAACCTGTACTGTTACTCGAAGGCTTTTTTGATGCCAGAGATCTGCCGACGATTTCTgc                                                                <  1:523756/140‑1 (MQ=255)
                                                                         cgcgAACGCTCCCCGATGCTGACGCCTTTGGCGTAGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGCGGGGGGG‑AATCACCAAACCTGTACTGTTACTCGAAGGCTTTTTTGATGCCAGAGATCTGCCGACGATTTCTgc                                                                >  1:245384/1‑140 (MQ=255)
                                                                         cgcgAACGCTCCCCGATGCTGACGCCTTTGGCGTAGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGCGGGGGGG‑AATCACCAAACCTGTACTGTTACTCGAAGGCTTTTTTGATGCCAGAGATCTGCCGACGATTTCTgc                                                                >  2:498556/1‑140 (MQ=255)
                                                                         cgcgAACGCTCCCCGATGCTGACGCCTTTGGCGTAGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGCGGGGGGG‑AATCACCAAACCTGTACTGTTACTCGAAGGCTTTTTTGATGCCAGAGATCTGCCGACGATTTCTgc                                                                >  2:1050368/1‑140 (MQ=255)
                                                                         cgcgAACGCTCCCCGATGCTGACGCCTTTGGCGTAGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGCGGGGGGG‑AATCACCAAACCTGTACTGTTACTCGAAGGCTTTTTTGATGCCAGAGATCTGCCGACGATTTCTgc                                                                >  1:8802/1‑140 (MQ=255)
                                                                         cgcgAACGCTCCCCGATGCTGACGCCTTTGGCGTAGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGCGGGGGGG‑AATCACCAAACCTGTACTGTTACTCGAAGGCTTTTTTGATGCCAGAGATCTGCCGACGATTTCTgc                                                                >  2:775975/1‑140 (MQ=255)
                                                                             aaCGCTCCCCGATGCTGACGCCTTTGGCGTAGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGCGGGGGGG‑AATCACCAAACCTGTACTGTTACTCGAAGGCTTTTTTGATGCCAGAGATCTGCCGACGATTTCTGCGCaa                                                            <  1:763525/140‑1 (MQ=255)
                                                                                       gATGCTGACGCCTTTGGCGTAGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGCGGGGGGG‑AATCACCAAACCTGTACTGTTACTCGAAGGCTTTTTTGATGCCAGAGATCTGCCGACGATTTCTGCGCAACATTTTCATa                                                  <  1:456869/140‑1 (MQ=255)
                                                                                       gATGCTGACGCCTTTGGCGTAGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGCGGGGGGG‑AATCACCAAACCTGTACTGTTACTCGAAGGCTTTTTTGATGCCAGAGATCTGCCGACGATTTCTGCGCAACATTTTCATa                                                  <  2:782062/140‑1 (MQ=255)
                                                                                                   tttGGCGTAGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGCGGGGGGG‑AATCACCAAACCTGTACTGTTACTCGAAGGCTTTTTTGATGCCAGAGATCTGCCGACGATTTCTGCGCAACATTTTCATACCGCCGTGCATa                                      >  2:617371/1‑140 (MQ=255)
                                                                                                    ttGGCGTAGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGCGGGGGGG‑AATCACCAAACCTGTACTGTTACTCGAAGGCTTTTTTGATGCCAGAGATCTGCCGACGATTTCTGCGCAACATTTTCATACCGCCGTGCATaa                                     <  1:617371/140‑1 (MQ=255)
                                                                                                      ggCGTAGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGCGGGGGGG‑AATCACCAAACCTGTACTGTTACTCGAAGGCTTTTTTGATGCCAGAGATCTGCCGACGATTTCTGCGCAACATTTTCATACCGCCGTGCATAACg                                   <  1:1050368/140‑1 (MQ=255)
                                                                                                                     gaagaaGCTCTGCGACTGCGTGCGGGGGGG‑AATCACCAAACCTGTACTGTTACTCGAAGGCTTTTTTGATGCCAGAGATCTGCCGACGATTTCTGCGCAACATTTTCATACCGCCGTGCATAACGAAGAACAgctggctg                    <  2:843256/140‑1 (MQ=255)
                                                                                                                        gaagCTCTGCGACTGCGTGCGGGGGGG‑AATCACCAAACCTGTACTGTTACTCGAAGGCTTTTTTGATGCCAGAGATCTGCCGACGATTTCTGCGCAACATTTTCATACCGCCGTGCATAACGAAGAACAGCTGGCTgcgc                 <  2:245384/140‑1 (MQ=255)
                                                                                                                            ctctGCGACTGCGTGCGGGGGGG‑AATCACCAAACCTGTACTGTTACTCGAAGGCTTTTTTGATGCCAGAGATCTGCCGACGATTTCTGCGCAACATTTTCATACCGCCGTGCATAACGAAGAACAGCTGGCTGCGCTGGa             >  2:577170/1‑140 (MQ=255)
                                                                                                                            ctctGCGACTGCGTGCGGGGGGG‑AATCACCAAACCTGTACTGTTACTCGAAGGCTTTTTTGATGCCAGAGATCTGCCGACGATTTCTGCGCAACATTTTCATACCGCCGTGCATAACGAAGAACAGCTGGCTGCGCTGGa             >  2:840659/1‑140 (MQ=255)
                                                                                                                            ctctGCGACTGCGTGCGGGGGGG‑AATCACCAAACCTGTACTGTTACTCGAAGGCTTTTTTGATGCCAGAGATCTGCCGACGATTTCTGCGCAACATTTTCATACCGCCGTGCATAACGAAGAACAGCTGGCTGCGCTGGa             <  1:775975/140‑1 (MQ=255)
                                                                                                                                       cgtgcgGGGGGG‑AATCACCAAACCTGTACTGTTACTCGAAGGCTTTTTTGATGCCAGAGATCTGCCGACGATTTCTGCGCAACATTTTCATACCGCCGTGCATAACGAAGAACAGCTGGCTGCGCTGGAAGAGGCTAGcc  >  2:1132863/1‑140 (MQ=255)
                                                                                                                                            |                                                                                                                                       
GAACTGGCCCCTGCCAGTAAAATGGTTGCGGTGGTGAAAGCGAACGCTTATGGTCACGGTCTTCTTGAGACCGCGCGAACGCTCCCCGATGCTGACGCCTTTGGCGTAGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGC‑GGGGGG‑AATCACCAAACCTGTACTGTTACTCGAAGGCTTTTTTGATGCCAGAGATCTGCCGACGATTTCTGCGCAACATTTTCATACCGCCGTGCATAACGAAGAACAGCTGGCTGCGCTGGAAGAGGCTAGCC  >  NC_000913/4265845‑4266118

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑