Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 355,356 (T)6→7 coding (435/1260 nt) codB → cytosine transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913355,3501.T98.4% 212.4 / ‑3.5 61coding (429/1260 nt)codBcytosine transporter
Reads supporting (aligned to +/- strand):  ref base . (1/0);  new base T (31/29);  total (32/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.98e-01

ACTGCTACTGGGCGGAACTCAGGTTGGCTGGTTTGGCGTCGGTGTGGCGATGTTTGCCATTCCGGTGGGTAAGGCAACCGGGCTGGATATTAATTTGCTGATTGCCGTTTCCGGTTTACTGATGACCGTCACCGTC‑T‑TTTTTGGCATTTCGGCGCTGACGGTTCTTTCGGTGATTGCGGTTCCGGCTATCGCCTGCCTGGGCGGTTATTCCGTGTGGCTGGCTGTTAACGGCATGGGCGGCCTGGACGCATTAAAAGCGGTCGTTCCCG  >  NC_000913/355215‑355483
                                                                                                                                        |                                                                                                                                      
aCTGCTACTGGGCGGAACTCAGGTTGGCTGGTTTGGCGTCGGTGTGGCGATGTTTGCCATTCCGGTGGGTAAGGCAACCGGGCTGGATATTAATTTGCTGATTGCCGTTTCCGGTTTACTGATGACCGTCACCGTC‑t‑ttt                                                                                                                                   >  2:66844/1‑140 (MQ=255)
     tACTGGGCGGAACTCAGGTTGGCTGGTTTGGCGTCGGTGTGGCGATGTTTGCCATTCCGGTGGGTAAGGCAACCGGGCTGGATATTAATTTGCTGATTGCCGTTTCCGGTTTACTGATGACCGTCACCGTC‑t‑ttttttgg                                                                                                                              >  2:232146/1‑137 (MQ=255)
      aCTGGGCGGAACTCAGGTTGGCTGGTTTGGCGTCGGTGTGGCGATGTTTGCCATTCCGGTGGGTAAGGCAACCGGGCTGGATATTAATTTGCTGATTGCCGTTTCCGGTTTACTGATGACCGTCACCGTC‑t‑ttttttggc                                                                                                                             >  2:1066066/1‑136 (MQ=255)
       cTGGGCGGAACTCAGGTTGGCTGGTTTGGCGTCGGTGTGGCGATGTTTGCCATTCCGGTGGGTAAGGCAACCGGGCTGGATATTAATTTGCTGATTGCCGTTTCCGGTTTACTGATGACCGTCACCGTC‑t‑ttttttggca                                                                                                                            >  1:851703/1‑135 (MQ=255)
        tGGGCGGAACTCAGGTTGGCTGGTTTGGCGTCGGTGTGGCGATGTTTGCCATTCCGGTGGGTAAGGCAACCGGGCTGGATATTAATTTGCTGATTGCCGTTTCCGGTTTACTGATGACCGTCACCGTCTT‑TTTTTGGCAt                                                                                                                            >  1:730619/1‑140 (MQ=255)
          ggCGGAACTCAGGTTGGCTGGTTTGGCGTCGGTGTGGCGATGTTTGCCATTCCGGTGGGTAAGGCAACCGGGCTGGATATTAATTTGCTGATTGCCGTTTCCGGTTTACTGATGACCGTCACCGTCTT‑TTTTTGGCAttt                                                                                                                          >  1:940153/1‑140 (MQ=255)
            cGGAACTCAGGTTGGCTGGTTTGGCGTCGGTGTGGCGATGTTTGCCATTCCGGTGGGTAAGGCAACCGGGCTGGATATTAATTTGCTGATTGCCGTTTCCGGTTTACTGATGACCGTCACCGTCTT‑TTTTTGGTATTTCg                                                                                                                        >  2:547684/1‑140 (MQ=255)
            cGGAACTCAGGTTGGCTGGTTTGGCGTCGGTGTGGCGATGTTTGCCATTCCGGTGGGTAAGGCAACCGGGCTGGATATTAATTTGCTGATTGCCGTTTCCGGTTTACTGATGACCGTCACCGTCTT‑TTTTTGGCATTTCg                                                                                                                        >  1:1186068/1‑140 (MQ=255)
             ggAACTCAGGTTGGCTGGTTTGGCGTCGGTGTGGCGATGTTTGCCATTCCGGTGGGTAAGGCAACCGGGCTGGATATTAATTTGCTGATTGCCGTTTCCGGTTTACTGATGACCGTCACCGTCTT‑TTTTTGGCATTTCgg                                                                                                                       <  1:81974/140‑1 (MQ=255)
              gAACTCAGGTTGGCTGGTTTGGCGTCGGTGTGGCGATGTTTGCCATTCCGGTGGGTAAGGCAACCGGGCTGGATATTAATTTGCTGATTGCCGTTTCCGGTTTACTGATGACCGTCACCGTCTT‑TTTTTGGCATTTCGgc                                                                                                                      <  2:730619/140‑1 (MQ=255)
               aaCTCAGGTTGGCTGGTTTGGCGTCGGTGTGGCGATGTTTGCCATTCCGGTGGGTAAGGCAACCGGGCTGGATATTAATTTGCTGATTGCCGTTTCCGGTTTACTGATGACCGTCACCGTCTT‑TTTTTGGCATTTCGgcg                                                                                                                     <  1:620757/140‑1 (MQ=255)
                 cTCAGGTTGGCTGGTTTGGCGTCGGTGTGGCGATGTTTGCCATTCCGGTGGGTAAGGCAACCGGGCTGGATATTAATTTGCTGATTGCCGTTTCCGGTTTACTGATGACCGTCACCGTCTT‑TTTTTGGCATTTCGGCGCt                                                                                                                   <  1:341573/140‑1 (MQ=255)
                   cAGGTTGGCTGGTTTGGCGTCGGTGTGGCGATGTTTGCCATTCCGGTGGGTAAGGCAACCGGGCTGGATATTAATTTGCTGATTGCCGTTTCCGGTTTACTGATGACCGTCACCGTCTT‑TTTTTGGCATTTCGGCGCTGa                                                                                                                 <  2:851703/140‑1 (MQ=255)
                    aGGTTGGCTGGTTTGGCGTCGGTGTGGCGATGTTTGCCATTCCGGTGGGTAAGGCAACCGGGCTGGATATTAATTTGCTGATTGCCGTTTCCGGTTTACTGATGACCGTCACCGTCTT‑TTTTTGGCATTTCGGCGCTGAc                                                                                                                >  2:358768/1‑140 (MQ=255)
                      gTTGGCTGGTTTGGCGTCGGTGTGGCGATGTTTGCCATTCCGGTGGGTAAGGCAACCGGGCTGGATATTAATTTGCTGATTGCCGTTTCCGGTTTACTGATGACCGTCACCGTCTT‑TTTTTGGCATTTCGGCGCTGACgg                                                                                                              >  1:713397/1‑140 (MQ=255)
                         ggCTGGTTTGGCGTCGGTGTGGCGATGTTTGCCATTCCGGTGGGTAAGGCAACCGGGCTGGATATTAATTTGCTGATTGCCGTTTCCGGTTTACTGATGACCGTCACCGTCTT‑TTTTTGGCATTTCGGCGCTGACGGTTc                                                                                                           >  2:587559/1‑140 (MQ=255)
                              gTTTGGCGTCGGTGTGGCGATGTTTGCCATTCCGGTGGGTAAGGCAACCGGGCTGGATATTAATTTGCTGATTGCCGTTTCCGGTTTACTGATGACCGTCACCGTCTT‑TTTTTGGCATTTCGGCGCTGACGGTTCTTTCg                                                                                                      <  2:96539/140‑1 (MQ=255)
                                 tGGCGTCGGTGTGGCGATGTTTGCCATTCCGGTGGGTAAGGCAACCGGGCTGGATATTAATTTGCTGATTGCCGTTTCCGGTTTACTGATGACCGTCACCGTCTT‑TTTTTGGCATTTCGGCGCTGACGGTTCTTTCGGTg                                                                                                   >  2:493347/1‑140 (MQ=255)
                                     gTCGGTGTGGCGATGTTTGCCATTCCGGTGGGTAAGGCAACCGGGCTGGATATTAATTTGCTGATTGCCGTTTCCGGTTTACTGATGACCGTCACCGTCTT‑TTTTTGGCATTTCGGCGCTGACGGTTCTTTCGGTGATTg                                                                                               <  2:1186068/140‑1 (MQ=255)
                                     gTCGGTGTGGCGATGTTTGCCATTCCGGTGGGTAAGGCAACCGGGCTGGATATTAATTTGCTGATTGCCGTTTCCGGTTTACTGATGACCGTCACCGTCTT‑TTTTTGGCATTTCGGCGCTGACGGTTCTTTCGGTGATTg                                                                                               <  1:493347/140‑1 (MQ=255)
                                       cGGTGTGGCGATGTTTGCCATTCCGGTGGGTAAGGCAACCGGGCTGGATATTAATTTGCTGATTGCCGTTTCCGGTTTACTGATGACCGTCACCGTCTT‑TTTTTGGCATTTCGGCGCTGACGGTTCTTTCGGTGATTGCg                                                                                             <  2:155703/140‑1 (MQ=255)
                                        ggTGTGGCGATGTTTGCCATTCCGGTGGGTAAGGCAACCGGGCTGGATATTAATTTGCTGATTGCCGTTTCCGGTTTACTGATGACCGTCACCGTCTT‑TTTTTGGCATTTCGGCGCTGACGGTTCTTTCGGTGATTGCgg                                                                                            >  1:644506/1‑140 (MQ=255)
                                         gtgtGGCGATGTTTGCCATTCCGGTGGGTAAGGCAACCGGGCTGGATATTAATTTGCTGATTGCCGTTTCCGGTTTACTGATGACCGTCACCGTCTT‑TTTTTGGCATTTCGGCGCTGACGGTTCTTTCGGTGATTGCGGt                                                                                           >  1:103461/1‑140 (MQ=255)
                                           gtgGCGATGTTTGCCATTCCGGTGGGTAAGGCAACCGGGCTGGATATTAATTTGCTGATTGCCGTTTCCGGTTTACTGATGACCGTCACCGTCTT‑TTTTTGGCATTTCGGCGCTGACGGTTCTTTCGGTGATTGCGGTTc                                                                                         <  2:203160/140‑1 (MQ=255)
                                             ggCGATGTTTGCCATTCCGGTGGGTAAGGCAACCGGGCTGGATATTAATTTGCTGATTGCCGTTTCCGGTTTACTGATGACCGTCACCGTCTT‑TTTTTGGCATTTCGGCGCTGACGGTTCTTTCGGTGATTGCGGTTCCg                                                                                       >  2:746274/1‑140 (MQ=255)
                                               cGATGTTTGCCATTCCGGTGGGTAAGGCAACCGGGCTGGATATTAATTTGCTGATTGCCGTTTCCGGTTTACTGATGACCGTCACCGTCTT‑TTTTTGGCATTTCGGCGCTGACGGTTCTTTCGGTGATTGCGGTTCCGGc                                                                                     <  2:178522/140‑1 (MQ=255)
                                                   gTTTGCCATTCCGGTGGGTAAGGCAACCGGGCTGGATATTAATTTGCTGATTGCCGTTTCCGGTTTACTGATGACCGTCACCGTCTT‑TTTTTGGCATTTCGGCGCTGACGGTTCTTTCGGTGATTGCGGTTCCGGCTATc                                                                                 >  1:823694/1‑140 (MQ=255)
                                                    tttGCCATTCCGGTGGGTAAGGCAACCGGGCTGGATATTAATTTGCTGATTGCCGTTTCCGGTTTACTGATGACCGTCACCGTCTT‑TTTTTGGCATTTCGGCGCTGACGGTTCTTTCGGTGATTGCGGTTCCGGCTATCg                                                                                <  2:46971/140‑1 (MQ=255)
                                                            tCCGGTGGGTAAGGCAACCGGGCTGGATATTAATTTGCTGATTGCCGTTTCCGGTTTACTGATGACCGTCACCGTCTT‑TTTTTGGCATTTCGGCGCTGACGGTTCTTTCGGTGATTGCGGTTCCGGCTATCGcctgcctg                                                                        <  2:211519/140‑1 (MQ=255)
                                                            tCCGGTGGGTAAGGCAACCGGGCTGGATATTAATTTGCTGATTGCCGTTTCCGGTTTACTGATGACCGTCACCGTCTT‑TTTTTGGCATTTCGGCGCTGACGGTTCTTTCGGTGATTGCGGTTCCGGCTATCGcctgcctg                                                                        <  1:1066066/140‑1 (MQ=255)
                                                            tCCGGTGGGTAAGGCAACCGGGCTGGATATTAATTTGCTGATTGCCGTTTCCGGTTTACTGATGACCGTCACCGTCTT‑TTTTTGGCATTTCGGCGCTGACGGTTCTTTCGGTGATTGCGGTTCCGGCTATCGcctgcctg                                                                        <  1:722803/140‑1 (MQ=255)
                                                              cGGTGGGTAAGGCAACCGGGCTGGATATTAATTTGCTGATTGCCGTTTCCGGTTTACTGATGACCGTCACCGTCTT‑TTTTTGGCATTTCGGCGCTGACGGTTCTTTCGGTGATTGCGGTTCCGGCTATCGCCTGCCTggg                                                                      <  1:232146/140‑1 (MQ=255)
                                                                gTGGGTAAGGCAACCGGGCTGGATATTAATTTGCTGATTGCCGTTTCCGGTTTACTGATGACCGTCACCGTCTT‑TTTTTGGCATTTCGGCGCTGACGGTTCTTTCGGTGATTGCGGTTCCGGCTATCGCCTGCCTGGGCg                                                                    >  1:945216/1‑140 (MQ=255)
                                                                     tAAGGCAACCGGGCTGGATATTAATTTGCTGATTGCCGTTTCCGGTTTACTGATGACCGTCACCGTCTT‑TTTTTGGCATTTCGGCGCTGACGGTTCTTTCGGTGATTGCGGTTCCGGCTATCGCCTGCCTggg                                                                      >  1:1029200/1‑133 (MQ=255)
                                                                     tAAGGCAACCGGGCTGGATATTAATTTGCTGATTGCCGTTTCCGGTTTACTGATGACCGTCACCGTCTT‑TTTTTGGCATTTCGGCGCTGACGGTTCTTTCGGTGATTGCGGTTCCGGCTATCGCCTGCCTggg                                                                      <  2:1029200/133‑1 (MQ=255)
                                                                        ggCAACCGGGCTGGATATTAATTTGCTGATTGCCGTTTCCGGTTTACTGATGACCGTCACCGTC‑TATTTTTGGCATTTCGGCGCTGACGGTTCTTTCGGTGATTGCGGTTCCGGCTATCGCCTGCCTGGGCGGTTATTcc                                                            >  1:1050789/1‑140 (MQ=255)
                                                                             ccGGGCTGGATATTAATTTGCTGATTGCCGTTTCCGGTTTACTGATGACCGTCACCGTCTT‑TTTTTGGCATTTCGGCGCTGACGGTTCTTTCGGTGATTGCGGTTCCGGCTATCGCCTGCCTGGGCg                                                                    <  2:650822/127‑1 (MQ=255)
                                                                             ccGGGCTGGATATTAATTTGCTGATTGCCGTTTCCGGTTTACTGATGACCGTCACCGTCTT‑TTTTTGGCATTTCGGCGCTGACGGTTCTTTCGGTGATTGCGGTTCCGGCTATCGCCTGCCTGGGCg                                                                    >  1:650822/1‑127 (MQ=255)
                                                                             ccGGGCTGGATATTAATTTGCTGATTGCCGTTTCCGGTTTACTGATGACCGTCACCGTCTT‑TTTTTGGCATTTCGGCGCTGACGGTTCTTTCGGTGATTGCGGTTCCGGCTATCGCCTGCCTGGGCGGTTATTCCGtgtg                                                       >  1:849211/1‑140 (MQ=255)
                                                                             ccGGGCTGGATATTAATTTGCTGATTGCCGTTTCCGGTTTACTGATGACCGTCACCGTCTT‑TTTTTGGCATTTCGGCGCTGACGGTTCTTTCGGTGATTGCGGTTCCGGCTATCGCCTGCCTGGGCGGTTATTCCGtgtg                                                       >  2:643716/1‑140 (MQ=255)
                                                                             ccGGGCTGGATATTAATTTGCTGATTGCCGTTTCCGGTTTACTGATGACCGTCACCGTCTT‑TTTTTGGCATTTCGGCGCTGACGGTTCTTTCGGTGATTGCGGTTCCGGCTATCGCCTGCCTGGGCGGTTATTCCGtgtg                                                       >  2:669335/1‑140 (MQ=255)
                                                                             ccGGGCTGGATATTAATTTGCTGATTGCCGTTTCCGGTTTACTGATGACCGTCACCGTCTT‑TTTTTGGCATTTCGGCGCTGACGGTTCTTTCGGTGATTGCGGTTCCGGCTATCGCCTGCCTGGGCGGTTATTCCGtgtg                                                       >  2:929812/1‑140 (MQ=255)
                                                                               gggCTGGATATTAATTTGCTGATTGCCGTTTCCGGTTTACTGATGACCGTCACCGTCTT‑TTTTTGGCATTTCGGCGCTGACGGTTCTTTCGGTGATTGCGGTTCCGGCTATCGCCTGCCTGGGCGGTTATTCCGTGtggc                                                     <  2:945216/140‑1 (MQ=255)
                                                                                  cTGGATATTAATTTGCTGATTGCCGTTTCCGGTTTACTGATGACCGTCACCGTCTT‑TTTTTGGCATTTCGGCGCTGACGGTTCTTTCGGTGATTGCGGTTCCGGCTATCGCCTGCCTGGGCGGTTATTCCGTGtggctgg                                                  >  1:540335/1‑140 (MQ=255)
                                                                                    ggATATTAATTTGCTGATTGCCGTTTCCGGTTTACTGATGACCGTCACCGTCTT‑TTTTTGGCATTTCGGCGCTGACGGTTCTTTCGGTGATTGCGGTTCCGGCTATCGCCTGCCTGGGCGGTTATTCCGTGTggctggct                                                <  1:587559/140‑1 (MQ=255)
                                                                                       tatTAATTTGCTGATTGCCGTTTCCGGTTTACTGATGACCGTCACCGTCTT‑TTTTTGGCATTTCGGCGCTGACGGTTCTTTCGGTGATTGCGGTTCCGGCTATCGCCTGCCTGGGCGGTTATTCCGTGTGGCTGGCTGtt                                             <  2:103461/140‑1 (MQ=255)
                                                                                        atTAATTTGCTGATTGCCGTTTCCGGTTTACTGATGACCGTCACCGTCTT‑TTTTTGGCATTTCGGCGCTGACGGTTCTTTCGGTGATTGCGGTTCCGGCTATCGCCTGCCTGGGCGGTTATTCCGTGTGGCTGGCTGTTa                                            >  1:1191420/1‑140 (MQ=255)
                                                                                             tttGCTGATTGCCGTTTCCGGTTTACTGATGACCGTCACCGTCTT‑TTTTTGGCATTTCGGCGCTGACGGTTCTTTCGGTGATTGCGGTTCCGGCTATCGCCTGCCTGGGCGGTTATTCCGTGTGGCTGGCTGTTAACGGc                                       <  1:214760/140‑1 (MQ=255)
                                                                                               tGCTGATTGCCGTTTCCGGTTTACTGATGACCGTCACCGTCTT‑TTTTTGGCATTTCGGCGCTGACGGTTCTTTCGGTGATTGCGGTTCCGGCTATCGCCTGCCTGGGCGGTTATTCCGTGTGGCTGGCTGTTAACGGCAt                                     <  2:849211/140‑1 (MQ=255)
                                                                                               tGCTGATTGCCGTTTCCGGTTTACTGATGACCGTCACCGTCTT‑TTTTTGGCATTTCGGCGCTGACGGTTCTTTCGGTGATTGCGGTTCCGGCTATCGCCTGCCTGGGCGGTTATTCCGTGTGGCTGGCTGTTAACGGCAt                                     >  1:985739/1‑140 (MQ=255)
                                                                                               tGCTGATTGCCGTTTCCGGTTTACTGATGACCGTCACCGTCTT‑TTTTTGGCATTTCGGCGCTGACGGTTCTTTCGGTGATTGCGGTTCCGGCTATCGCCTGCCTGGGCGGTTATTCCGTGTGGCTGGCTGTTAACGGCAt                                     >  2:196828/1‑140 (MQ=255)
                                                                                               tGCTGATTGCCGTTTCCGGTTTACTGATGACCGTCACCGTCTT‑TTTTTGGCATTTCGGCGCTGACGGTTCTTTCGGTGATTGCGGTTCCGGCTATCGCCTGCCTGGGCGGTTATTCCGTGTGGCTGGCTGTTAACGGCAt                                     >  1:216560/1‑140 (MQ=255)
                                                                                               tGCTGATTGCCGTTTCCGGTTTACTGATGACCGTCACCGTCTT‑TTTTTGGCATTTCGGCGCTGACGGTTCTTTCGGTGATTGCGGTTCCGGCTATCGCCTGCCTGGGCGGTTATTCCGTGTGGCTGGCTGTTAACGGCAt                                     >  1:501891/1‑140 (MQ=255)
                                                                                                 cTGATTGCCGTTTCCGGTTTACTGATGACCGTCACCGTCTT‑TTTTTGGCATTTCGGCGCTGACGGTTCTTTCGGTGATTGCGGTTCCGGCTATCGCCTGCCTGGGCGGTTATTCCGTGTGGCTGGCTGTTAACGGCATgg                                   >  1:325575/1‑140 (MQ=255)
                                                                                                 cTGATTGCCGTTTCCGGTTTACTGATGACCGTCACCGTCTT‑TTTTTGGCAATTCGGCGCTGACGGTTCTTTCGGTGATTGCGGTTCCGGCTATCGCCTGCCTGGGCGGTTATTCCGTGTGGCTGGCTGTTAACGGCATgg                                   >  2:413909/1‑140 (MQ=255)
                                                                                                  tGATTGCCGTTTCCGGTTTACTGATGACCGTCACCGTCTT‑TTTTTGGCATTTCGGCGCTGACGGTTCTTTCGGTGATTGCGGTTCCGGCTATCGCCTGCCTGGGCGGTTATTCCGTGTGGCTGGCTGTTAACGGCATggg                                  >  1:196202/1‑140 (MQ=255)
                                                                                                  tGATTGCCGTTTCCGGTTTACTGATGACCGTCACCGTCTT‑TTTTTGGCATTTCGGCGCTGACGGTTCTTTCGGTGATTGCGGTTCCGGCTATCGCCTGCCTGGGCGGTTATTCCGTGTGGCTGGCTGTTAACGGCATggg                                  >  1:900619/1‑140 (MQ=255)
                                                                                                   gATTGCCGTTTCCGGTTTACTGATGACCGTCACCGTCTT‑TTTTTGGCATTTCGGCGCTGACGGTTCTTTCGGTGATTGCGGTTCCGGCTATCGCCTGCCTGGGCGGTTATTCCGTGTGGCTGGCTGTTAACGGCATGggc                                 <  1:321775/140‑1 (MQ=255)
                                                                                                      tGCCGTTTCCGGTTTACTGATGACCGTCACCGTCTT‑TTTTTGGCATTTCGGCGCTGACGGTTCTTTCGGTGATTGCGGTTCCGGCTATCGCCTGCCTGGGCGGTTATTCCGTGTGGCTGGCTGTTAACGGCATGggcggc                              >  2:976868/1‑140 (MQ=255)
                                                                                                           tttCCGGTTTACTGATGACCGTCACCGTCTT‑TTTTTGGCATTTCGGCGCTGACGGTTCTTTCGGTGATTGCGGTTCCGGCTATCGCCTGCCTGGGCGGTTATTCCGTGTGGCTGGCTGTTAACGGCATGGGCGGCCTGGa                         <  1:174133/140‑1 (MQ=255)
                                                                                                           tttCCGGTTTACTGATGACCGTCACCGTCTT‑TTTTTGGCATTTCGGCGCTGACGGTTCTTTCGGTGATTGCGGTTCCGGCTATCGCCTGCCTGGGCGGTTATTCCGTGTGGCTGGCTGTTAACGGCATGGGCGGCCTGGa                         <  2:644506/140‑1 (MQ=255)
                                                                                                               cGGTTTACTGATGACCGTCACCGTCTT‑TTTTTGGCATTTCGGCGCTGACGGTTCTTTCGGTGATTGCGGTTCCGGCTATCGCCTGCCTGGGCGGTTATTCCGTGTGGCTGGCTGTTAACGGCATGGGCGGCCTGGACGCa                     <  2:357374/140‑1 (MQ=255)
                                                                                                                 gTTTACTGATGACCGTCACCGTCTT‑TTTTTGGCATTTCGGCGCTGACGGTTCTTTCGGTGATTGCGGTTCCGGCTATCGCCTGCCTGGGCGGTTATTCCGTGTGGCTGGCTGTTAACGGCATGGGCGGCCTGGACGCAtt                   <  2:1131480/140‑1 (MQ=255)
                                                                                                                   ttACTGATGACCGTCACCGTCTT‑TTTTTGGCATTTCGGCGCTGACGGTTCTTTCGGTGATTGCGGTTCCGGCTATCGCCTGCCTGGGCGGTTATTCCGTGTGGCTGGCTGTTAACGGCATGGGCGGCCTGGACGCATTaa                 <  1:8606/140‑1 (MQ=255)
                                                                                                                           gaCCGTCACCGTCTT‑TTTTTGGCATTTCGGCGCTGACGGTTCTTTCGGTGATTGCGGTTCCGGCTATCGCCTGCCTGGGCGGTTATTCCGTGTGGCTGGCTGTTAACGGCATGGGCGGCCTGGACGCATTAAAAGCGGTc         >  2:867766/1‑140 (MQ=255)
                                                                                                                            accgtcaccgtctT‑TTTTTGGCATTTCGGCGCTGACGGTTCTTTCGGTGATTGCGGTTCCGGCTATCGCCTGCCTGGGCGGTTATTCCGTGTGGCTGGCTGTTAACGGCATGGGCGGCCTGGACGCATTAAAAGCGGTCg        >  2:354449/1‑140 (MQ=255)
                                                                                                                              cgtcaccgtctT‑TTTTTGGCATTTCGGCGCTGACGGTTCTTTCGGTGATTGCGGTTCCGGCTATCGCCTGCCTGGGCGGTTATTCCGTGTGGCTGGCTGTTAACGGCATGGGCGGCCTGGACGCATTAAAAGCGGTCGtt      >  1:91556/1‑140 (MQ=255)
                                                                                                                              cgtcaccgtctT‑TTTTTGGCATTTCGGCGCTGACGGTTCTTTCGGTGATTGCGGTTCCGGCTATCGCCTGCCTGGGCGGTTATTCCGTGTGGCTGGCTGTTAACGGCATGGGCGGCCTGGACGCATTAAAAGCGGTCGtt      >  1:192960/1‑140 (MQ=255)
                                                                                                                              cgtcaccgtctT‑TTTTTGGCATTTCGGCGCTGACGGTTCTTTCGGTGATTGCGGTTCCGGCTATCGCCTGCCTGGGCGGTTATTCCGTGTGGCTGGCTGTTAACGGCATGGGCGGCCTGGACGCATTAAAAGCGGTCGtt      >  2:413523/1‑140 (MQ=255)
                                                                                                                                tcaccgtctT‑TTTTTGGCATTTCGGCGCTGACGGTTCTTTCGGTGATTGCGGTTCCGGCTATCGCCTGCCTGGGCGGTTATTCCGTGTGGCTGGCTGTTAACGGCATGGGCGGCCTGGACGCATTAAAAGCGGt          >  1:706206/1‑134 (MQ=255)
                                                                                                                                tcaccgtctT‑TTTTTGGCATTTCGGCGCTGACGGTTCTTTCGGTGATTGCGGTTCCGGCTATCGCCTGCCTGGGCGGTTATTCCGTGTGGCTGGCTGTTAACGGCATGGGCGGCCTGGACGCATTAAAAGCGGt          <  2:706206/134‑1 (MQ=255)
                                                                                                                                tcaccgtctT‑TTTTTGGCATTTCGGCGCTGACGGTTCTTTCGGTGATTGCGGTTCCGGCTATCGCCTGCCTGGGCGGTTATTCCGTGTGGCTGGCTGTTAACGGCATGGGCGGCCTGGACGCATTAAAAGCGGTCGTTcc    <  2:1191420/140‑1 (MQ=255)
                                                                                                                                tcaccgtctT‑TTTTTGGCATTTCGGCGCTGACGGTTCTTTCGGTGATTGCGGTTCCGGCTATCGCCTGCCTGGGCGGTTATTCCGTGTGGCTGGCTGTTAACGGCATGGGCGGCCTGGACGCATTAAAAGCGGTCGTTcc    >  1:1134661/1‑140 (MQ=255)
                                                                                                                                  accgtctT‑TTTTTGGCATTTCGGCGCTGACGGTTCTTTCGGTGATTGCGGTTCCGGCTATCGCCTGCCTGGGCGGTTATTCCGTGTGGCTGGCTGTTAACGGCATGGGCGGCCTGGACGCATTAAAAGCGGTCGTTCCCg  <  1:867766/140‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                      
ACTGCTACTGGGCGGAACTCAGGTTGGCTGGTTTGGCGTCGGTGTGGCGATGTTTGCCATTCCGGTGGGTAAGGCAACCGGGCTGGATATTAATTTGCTGATTGCCGTTTCCGGTTTACTGATGACCGTCACCGTC‑T‑TTTTTGGCATTTCGGCGCTGACGGTTCTTTCGGTGATTGCGGTTCCGGCTATCGCCTGCCTGGGCGGTTATTCCGTGTGGCTGGCTGTTAACGGCATGGGCGGCCTGGACGCATTAAAAGCGGTCGTTCCCG  >  NC_000913/355215‑355483

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑