Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,572,093	(T)6→7	intergenic (+178/‑321)	yjiS → / → yjiT	DUF1127 domain‑containing protein YjiS/putative uncharacterized protein YjiT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,572,087	1	.	T	100.0%	280.4 / NA	78	intergenic (+172/‑327)	yjiS/yjiT	DUF1127 domain‑containing protein YjiS/putative uncharacterized protein YjiT
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (50/28);  total (50/28)

TTAAATTAAGTTATAAAAATTTTCCCGAGACAATTCATCAATAGGTATGGAGTGTACAGGAATATCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATTACTTGTG‑TTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTAAATGTCAGCTACTGAATACTTTTTGATTGTTTGAGATTTATTTTCATTTGAAATTATA  >  NC_000913/4571958‑4572221                                                                                                                                   |                                                                                                                                       ttAAATTAAGTTATAAAAATTTTCCCGAGACAATTCATCAATAGGTATGGAGTGTACAGGAATATCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATTACTTGTG‑tttttttgac                                                                                                                              <  1:555794/140‑5 (MQ=255)  tAAATTAAGTTATAAAAATTTTCCCGAGACAATTCATCAATAGGTATGGAGTGTACAGGAATATCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATTACTTGTG‑tttttttgact                                                                                                                             <  1:1094725/140‑6 (MQ=255)  tAAATTAAGTTATAAAAATTTTCCCGAGACAATTCATCAATAGGTATGGAGTGTACAGGAATATCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATTACTTGTG‑tttttttgact                                                                                                                             <  2:1123618/140‑6 (MQ=255)  tAAATTAAGTTATAAAAATTTTCCCGAGACAATTCATCAATAGGTATGGAGTGTACAGGAATATCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATTACTTGTG‑tttttttgact                                                                                                                             <  1:861288/140‑6 (MQ=255)         aGTTATAAAAATTTTCCCGAGACAATTCATCAATAGGTATGGAGTGTACAGGAATATCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATTACTTGTG‑tttttttgact                                                                                                                             <  2:576363/133‑6 (MQ=255)         aGTTATAAAAATTTTCCCGAGACAATTCATCAATAGGTATGGAGTGTACAGGAATATCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATTACTTGTG‑tttttttgact                                                                                                                             >  1:576363/1‑128 (MQ=255)            tataAAAATTTTCCCGAGACAATTCATCAATAGGTATGGAGTGTACAGGAATATCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATTACTTGTGTTTTTTTGACTGACTTTACaa                                                                                                                    <  1:210634/140‑1 (MQ=255)                  aaTTTTCCCGAGACAATTCATCAATAGGTATGGAGTGTACAGGAATATCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATTACTTGTGTTTTTTTGACTGACTTTACAACTCTCa                                                                                                              <  1:31614/140‑1 (MQ=255)                                 aTTCATCAATAGGTATGGAGTGTACAGGAATATCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATTACTTGTGTTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATtgtg                                                                                               <  1:906386/140‑1 (MQ=255)                                   tcatcaATAGGTATGGAGTGTACAGGAATATCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATTACTTGTGTTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCa                                                                                             <  2:619885/140‑1 (MQ=255)                                             gTATGGAGTGTACAGGAATATCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATTACTTGTGTTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTaa                                                                                   <  2:530275/140‑1 (MQ=255)                                             gTATGGAGTGTACAGGAATATCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATTACTTGTGTTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTaa                                                                                   <  2:216466/140‑1 (MQ=255)                                             gTATGGAGTGTACAGGAATATCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATTACTTGTGTTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTaa                                                                                   >  1:193833/1‑140 (MQ=255)                                               aTGGAGTGTACAGGAATATCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATTACTTGTGTTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAAtt                                                                                 >  1:220371/1‑140 (MQ=255)                                                 ggAGTGTACAGGAATATCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATTACTTGTGTTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGt                                                                               >  1:592055/1‑140 (MQ=255)                                                     tgtACAGGAATATCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATTACTTGTGTTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTta                                                                           <  1:1082745/140‑1 (MQ=255)                                                     tgtACAGGAATATCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATTACTTGTGTTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTta                                                                           <  2:592055/140‑1 (MQ=255)                                                       tACAGGAATATCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATTACTTGTGTTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGtt                                                                                          >  1:960127/1‑123 (MQ=255)                                                       tACAGGAATATCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATTACTTGTGTTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGtt                                                                                          <  2:960127/123‑1 (MQ=255)                                                       tACAGGAATATCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATTACTTGTGTTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTtc                                                                                        <  1:116911/125‑1 (MQ=255)                                                       tACAGGAATATCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATTACTTGTGTTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTtc                                                                                        >  2:116911/1‑125 (MQ=255)                                                       tACAGGAATATCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATTACTTGTGTTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTaa                                                                                   <  2:608077/130‑1 (MQ=255)                                                       tACAGGAATATCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATTACTTGTGTTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTaa                                                                                   >  1:608077/1‑130 (MQ=255)                                                       tACAGGAATATCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATTACTTGTGTTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGtttt                                                                            >  1:267231/1‑137 (MQ=255)                                                       tACAGGAATATCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATTACTTGTGTTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGtttt                                                                            <  2:267231/137‑1 (MQ=255)                                                       tACAGGAATATCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATTACTTGTGTTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGtt                                                                              <  2:227987/135‑1 (MQ=255)                                                       tACAGGAATATCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATTACTTGTGTTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGtt                                                                              >  1:227987/1‑135 (MQ=255)                                                       tACAGGAATATCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATTACTTGTGTTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTtata                                                                         >  1:852508/1‑140 (MQ=255)                                                       tACAGGAATATCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATTACTTGTGTTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTtata                                                                         >  2:550284/1‑140 (MQ=255)                                                       tACAGGAATATCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATTACTTGTGTTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTtata                                                                         <  1:111986/140‑1 (MQ=255)                                                       tACAGGAATATCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATTACTTGTGTTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTtata                                                                         >  2:736347/1‑140 (MQ=255)                                                       tACAGGAATATCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATTACTTGTGTTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTtata                                                                         >  1:161239/1‑140 (MQ=255)                                                       tACAGGAATATCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATTACTTGTGTTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTtata                                                                         >  2:10443/1‑140 (MQ=255)                                                        aCAGGAATATCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATTACTTGTGTTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATAc                                                                        >  1:181370/1‑140 (MQ=255)                                                            gAATATCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATTACTTGTGTTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTg                                                                    >  1:225664/1‑140 (MQ=255)                                                            gAATATCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATTACTTGTGTTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTg                                                                    >  2:411165/1‑140 (MQ=255)                                                              atatCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATTACTTGTGTTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGa                                                                  <  2:193833/140‑1 (MQ=255)                                                               tatCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATTACTTGTGTTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTta                                                                           >  2:280705/1‑130 (MQ=255)                                                               tatCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATTACTTGTGTTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTta                                                                           <  1:280705/130‑1 (MQ=255)                                                               tatCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATTACTTGTGTTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGaa                                                                 >  2:774448/1‑140 (MQ=255)                                                               tatCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATTACTTGTGTTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGaa                                                                 >  2:1097089/1‑140 (MQ=255)                                                               tatCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATTACTTGTGTTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGaa                                                                 >  1:331454/1‑140 (MQ=255)                                                               tatCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATTACTTGTGTTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGaa                                                                 >  2:915553/1‑140 (MQ=255)                                                                      ttcACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATTACTTGTGTTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGaa                                                                 >  1:1135625/1‑133 (MQ=255)                                                                      ttcACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATTACTTGTGTTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGaa                                                                 <  2:1135625/133‑1 (MQ=255)                                                                      ttcACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATTACTTGTGTTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGa                                                                  <  1:768754/132‑1 (MQ=255)                                                                      ttcACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATTACTTGTGTTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGa                                                                  >  2:768754/1‑132 (MQ=255)                                                                      ttcACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATTACTTGTGTTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTaaa                                                          >  2:416065/1‑140 (MQ=255)                                                                      ttcACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATTACTTGTGTTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTaaa                                                          >  2:321676/1‑140 (MQ=255)                                                                      ttcACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATTACTTGTGTTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTaaa                                                          >  2:300979/1‑140 (MQ=255)                                                                      ttcACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATTACTTGTGTTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTaaa                                                          >  2:897719/1‑140 (MQ=255)                                                                      ttcACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATTACTTGTGTTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTaaa                                                          >  1:10304/1‑140 (MQ=255)                                                                      ttcACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATTACTCGTGTTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTaaa                                                          >  1:665620/1‑140 (MQ=255)                                                                      ttcACTAACCTCTTTAAACTCATGTCGATAACAGTCAAAAAACGCACAGTGATTACTTGTGTTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTaaa                                                          >  2:14329/1‑140 (MQ=255)                                                                         aCTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATTACTCGTGTTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTAAATGt                                                       <  2:665620/140‑1 (MQ=255)                                                                            aaCCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATTACTTGTGTTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTAAATGTCAg                                                    <  1:343253/140‑1 (MQ=255)                                                                             aCCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATTACTTGTGTTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTAAATGTCAGc                                                   >  2:285290/1‑140 (MQ=255)                                                                                  tttAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATTACTTGTGTTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTAAATGTCAGCTACTg                                              >  1:319570/1‑140 (MQ=255)                                                                                  tttAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATTACTTGTGTTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTAAATGTCAGCTACTg                                              >  2:4226/1‑140 (MQ=255)                                                                                   ttAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATTACTTGTGTTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTAAATGTCAGCTACTGa                                             >  1:1185059/1‑140 (MQ=255)                                                                                    tAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATTACTTGTGTTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTAAATGTCAGCTACTGaa                                            <  1:736347/140‑1 (MQ=255)                                                                                     aaaCTCATGTCGATAACGGTCAAAAAACGCACAGTGATTACTTGTGTTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTAAATGTCAGCTACTGAAt                                           >  2:320134/1‑140 (MQ=255)                                                                                       aCTCATGTCGATAACGGTCAAAAAACGCACAGTGATTACTTGTGTTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTAAATGTCAGCTACTGAATAc                                         >  1:265291/1‑140 (MQ=255)                                                                                           aTGTCGATAACGGTCAAAAAACGCACAGTGATTACTTGTGTTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTAAATGTCAGCTACTGAATACtttt                                     >  1:207973/1‑140 (MQ=255)                                                                                           aTGTCGATAACGGTCAAAAAACGCACAGTGATTACTTGTGTTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTAAATGTCAGCTACTGAATACtttt                                     >  1:185686/1‑140 (MQ=255)                                                                                            tGTCGATAACGGTCAAAAAACGCACAGTGATTACTTGTGTTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTAAATGTCAGCTACTGaa                                            >  1:6325/1‑132 (MQ=255)                                                                                            tGTCGATAACGGTCAAAAAACGCACAGTGATTACTTGTGTTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTAAATGTCAGCTACTGaa                                            <  2:6325/132‑1 (MQ=255)                                                                                            tGTCGATAACGGTCAAAAAACGCACAGTGATTACTTGTGTTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTAAATGTCAGCTACTGaa                                            <  2:1151879/132‑1 (MQ=255)                                                                                            tGTCGATAACGGTCAAAAAACGCACAGTGATTACTTGTGTTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTAAATGTCAGCTACTGaa                                            >  1:1151879/1‑132 (MQ=255)                                                                                            tGTCGATAACGGTCAAAAAACGCACAGTGATTACTTGTGTTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTAAATGTCAGCTACTGAATACttttt                                    >  2:428601/1‑140 (MQ=255)                                                                                            tGTCGATAACGGTCAAAAAACGCACAGTGATTACTTGTGTTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTAAATGTCAGCTACTGAATACttttt                                    >  1:667574/1‑140 (MQ=255)                                                                                                     cGGTCAAAAAACGCACAGTGATTACTTGTGTTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTAAATGTCAGCTACTGAATACTTTTTGATTGt                              >  2:231849/1‑137 (MQ=255)                                                                                                     cGGTCAAAAAACGCACAGTGATTACTTGTGTTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTAAATGTCAGCTACTGAATACTTTTTGATTGt                              <  1:231849/137‑1 (MQ=255)                                                                                                     cGGTCAAAAAACGCACAGTGATTACTTGTGTTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTAAATGTCAGCTACTGAATACTTTTTGATTGTTTg                           >  1:1096146/1‑140 (MQ=255)                                                                                                       gTCAAAAAACGCACAGTGATTACTTGTGTTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTAAATGTCAGCTACTGAATACTTTTTGATTGTTTgag                         >  2:393499/1‑140 (MQ=255)                                                                                                       gTCAAAAAACGCACAGTGATTACTTGTGTTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTAAATGTCAGCTACTGAATACTTTTTGATTGTTTgag                         >  2:657085/1‑140 (MQ=255)                                                                                                        tCAAAAAACGCACAGTGATTACTTGTGTTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTAAATGTCAGCTACTGAATACTTTTTGATTGTTTgaga                        >  2:169131/1‑140 (MQ=255)                                                                                                              aaCGCACAGTGATTACTTGTGTTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTAAATGTCAGCTACTGAATACTTTTTGATTGTTTGAGatttatt                  <  1:915553/140‑1 (MQ=255)                                                                                                              aaCGCACAGTGATTACTTGTGTTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTAAATGTCAGCTACTGAATACTTTTTGATTGTTTGAGatttatt                  <  2:181370/140‑1 (MQ=255)                                                                                                                  cacaGTGATTACTTGTGTTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTAAATGTCATCTACTGAATACTTTTTGTTTGTTTGAGATTTATTTTCa              >  2:1007269/1‑140 (MQ=255)                                                                                                                      gTGATTACTTGTGTTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTAAATGTCAGCTACTGAATACTTTTTGATTGTTTGAGATTTATTTTCATTTg          >  2:356145/1‑140 (MQ=255)                                                                                                                        gATTACTTGTGTTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTAAATGTCAGCTACTGAATACTTTTTGATTGTTTGAGATTTATTTTCATTTGaa        <  2:185686/140‑1 (MQ=255)                                                                                                                          ttACTTGTGTTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTAAATGTCAGCTACTGAATACTTTTTGATTGTTTGAGATTTATTTTCATTTGAAAt      >  1:865760/1‑140 (MQ=255)                                                                                                                              ttGTGTTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTAAATGTCAGCTACTGAATACTTTTTGATTGTTTGAGATTTATTTTCATTTGAAATtata  <  1:663682/140‑1 (MQ=255)                                                                                                                                   |                                                                                                                                       TTAAATTAAGTTATAAAAATTTTCCCGAGACAATTCATCAATAGGTATGGAGTGTACAGGAATATCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATTACTTGTG‑TTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTAAATGTCAGCTACTGAATACTTTTTGATTGTTTGAGATTTATTTTCATTTGAAATTATA  >  NC_000913/4571958‑4572221

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑